Laboratory Animals最新文献

A customised combination of environmental enrichment reduces aggression in CD-1 male mice. 定制的环境富集组合减少了CD-1雄性小鼠的攻击性。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-07-21 DOI: 10.1177/00236772251333986

Amy Veness, Christophe Galichet, Sian Murphy, Tina O'Mahony, Yoh Isogai, Eleni M Amaniti

引用次数: 0

A scoping review on reporting of methods in DSS colitis mouse models. DSS结肠炎小鼠模型研究方法综述。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-14 DOI: 10.1177/00236772251331677

Michaela Thallmair, Paulin Jirkof

{"title":"A scoping review on reporting of methods in DSS colitis mouse models.","authors":"Michaela Thallmair, Paulin Jirkof","doi":"10.1177/00236772251331677","DOIUrl":"https://doi.org/10.1177/00236772251331677","url":null,"abstract":"Inflammatory bowel diseases (IBDs) are a major health burden, and incidence as well as prevalence have increased over the last decades. Colitis animal models are used to explore the underlying pathogenesis of and therapeutic options for IBD. Since the reporting of specific aspects of colitis studies using mice has been criticized in the past, we performed a scoping review based on the PRISMA guidelines to assess putative improvements in the quality of reporting. A defined search for dextran sulfate sodium (DSS) murine colitis models was performed in three literature databases (PubMed, PubMed Central®, and Embase) for two time periods: 2007/2008 and 2017. Data were extracted from 122 articles published in 2007/2008 and 236 publications from 2017. We checked the articles for the reporting of details of the colitis model (DSS properties, manufacturer, concentration, duration of application, mouse strain, sex, source), measures to reduce allocation, performance and detection bias (randomization and blinding), and information on clinical assessment, refinements, and humane endpoints. Our results showed that there was significant improvement over time in the scores for refinement and, based on this, also the completeness score. However, the other aspects were poorly reported, suggesting that this research field may need to adopt reporting guidelines such as ARRIVE, the Gold Standard Publication Checklist, or the colitis methods checklist when writing and reviewing publications.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251331677"},"PeriodicalIF":1.3,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The (re)turn of the 3Rs: an inquiry into the normative nature of Russell and Burch's principles of humane experimental technique - their misunderstanding, reform and implementation through an ethics tool. 3r的（再）转向：探究罗素和伯奇的人道实验技术原则的规范性——他们的误解、改革和通过伦理工具的实施。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-12 DOI: 10.1177/00236772251326352

Herwig Grimm, Marc Dusseldorp

{"title":"The (re)turn of the 3Rs: an inquiry into the normative nature of Russell and Burch's principles of humane experimental technique - their misunderstanding, reform and implementation through an ethics tool.","authors":"Herwig Grimm, Marc Dusseldorp","doi":"10.1177/00236772251326352","DOIUrl":"https://doi.org/10.1177/00236772251326352","url":null,"abstract":"The 3Rs strongly shape the practice of laboratory animal use, as well as related policies worldwide. This success should not obscure the fact that implementing the 3Rs comes with challenges. A major problem is that it is fundamentally unclear under which conditions the 3Rs may be considered fulfilled in specific contexts. We argue that this lack of clarity is largely a result of the fact that the normative nature of the 3Rs has so far been disregarded. Hence, this paper seeks to answer the following research question: how is the normative nature of the 3Rs to be understood, and how can this understanding transparently guide their implementation? Based on a distinction between different types of norms, we show that the 3Rs, which have been called 'principles' since their origin, are indeed to be understood as principles in a substantive (norm-theoretical) sense. That is, they are norms that command the highest possible realization of their content. This understanding of the normative nature of the 3Rs has a significant effect on their implementation in practical contexts. As we will argue, it turns the orthodox idea of implementation strategies upside down. Building on this theoretical claim, we propose an ethics tool designed to help applicants, review committee members and authorities to apply the 3Rs transparently and, above all, in accordance with a reflected understanding of the relevant EU Directive's intention (Directive 2010/63/EU) and of the work of Russell and Burch, the pioneers of this milestone in the promotion of animal welfare in research.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251326352"},"PeriodicalIF":1.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Teaching experimental design: outputs from the FELASA Working Group. 教学实验设计：FELASA工作组的产出。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-09 DOI: 10.1177/00236772241295308

Derek Fry, Manuel Berdoy, Monica Forni, Carlos Oscar S Sorzano, Thomas Steckler, Nuno H Franco

{"title":"Teaching experimental design: outputs from the FELASA Working Group.","authors":"Derek Fry, Manuel Berdoy, Monica Forni, Carlos Oscar S Sorzano, Thomas Steckler, Nuno H Franco","doi":"10.1177/00236772241295308","DOIUrl":"https://doi.org/10.1177/00236772241295308","url":null,"abstract":"Good education and training for scientists undertaking animal experiments is important for providing understanding of key issues in experimental design (ED) and for alleviating continuing concerns about the conduct of animal and in vitro research studies. We present here outputs from the FELASA Experimental Design Working Group, set up to consider the current provision of ED teaching and how it might be improved and harmonised across the laboratory animal science community. It is hoped that these outputs will provide practical help to ED teachers who wish to enhance the effectiveness of their teaching; they include• A list of learning outcomes (LOs) that should be achieved by learners, principally aimed at early career researchers;• An example of an (adaptable) template of how these LOs could be addressed in 16 h (12 h tuition plus breaks), ideally as a 2-day workshop. If circumstances make 12 h tuition impossible to achieve, key LOs for a shorter course are identified;• Guidance and recommendations for running ED courses, including some ideas for achieving effective learning, the ideal skill set for tutors, some teaching scenarios, and the amount of statistics to have in a basic experimental design course;• A glossary of relevant terms (in supplemental material);• A description of how the 2-day course format ran on two trial occasions, with results of informal assessment of participants as well as their feedback, both immediate and a year afterwards, indicating it was effective;• A programme for a potential 2-day, training-the-trainers style, workshop, describing its key elements and the results of trialling this with a range of ED tutors.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241295308"},"PeriodicalIF":1.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating cervical dislocation methods, without using tension on the tail, for humanely killing adult laboratory mice. 评价在不使用尾部张力的情况下，颈椎脱臼法对成年实验小鼠的人道杀伤效果。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-09 DOI: 10.1177/00236772251332722

Antony Davidge, Flaviu Bulat, Aude Vernet

引用次数: 0

Sex and strain differences in the micropipette-guided drug administration (MDA) method in mice. 小鼠微管引导给药（MDA）方法的性别和品系差异。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-03 DOI: 10.1177/00236772251320843

Sarah Steiner, Olga Krzyzaniak, Frida A M Nilsson, Macsmeila Dietrich, Laura Kämpfen, Pål Johansen, Paulin Jirkof, Urs Meyer

{"title":"Sex and strain differences in the micropipette-guided drug administration (MDA) method in mice.","authors":"Sarah Steiner, Olga Krzyzaniak, Frida A M Nilsson, Macsmeila Dietrich, Laura Kämpfen, Pål Johansen, Paulin Jirkof, Urs Meyer","doi":"10.1177/00236772251320843","DOIUrl":"https://doi.org/10.1177/00236772251320843","url":null,"abstract":"To provide an alternative to oral gavage for per os treatments in laboratory mice, we have recently developed and introduced the micropipette-guided drug administration (MDA) method. This procedure is based on the presentation of a palatable solution consisting of sweetened condensed milk diluted with water, encouraging voluntary consumption of the vehicle and drug formulations. In this study, we compared the MDA method in male and female C57BL/6N and BALB/c mice, two inbred strains widely used in basic and preclinical research. Administering a diluted condensed milk solution daily over a period of 30 days, we observed that male C57BL/6N mice reached the fully voluntary drinking stage within five days or less, requiring the least amount of time to drink from the micropipette. Compared with males, female C57BL/6N mice showed increased consumption times during the initial administration period, yet they all managed to reach a fully voluntary stage within seven days or less. By contrast, BALB/c mice in general, and female BALB/c mice in particular, consistently required more time to consume the diluted condensed milk solution, whether administered voluntarily (no restraint) or semi-voluntarily (with mild tail restraint). Notably, a substantial portion of BALB/c mice (12.5% of males and 62.5% of females) failed to achieve fully voluntary consumption by MDA, despite their ability and willingness to drink the condensed milk solution when using a mild tail restraint. Taken together, the present study identified significant strain and sex differences in the MDA method as applied to laboratory mice.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251320843"},"PeriodicalIF":1.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

News from the EIC team. 来自EIC团队的消息。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-05-30 DOI: 10.1177/00236772251338015

Annemarie Lang, Paulin Jirkof, Josè Sanchez-Morgado, Jordi L Tremoleda

引用次数: 0

Cellular immune response against rabies vaccination in laboratory-bred dogs: A systematised review. 细胞免疫反应对抗狂犬病疫苗在实验室培育的狗：一个系统的回顾。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-25 DOI: 10.1177/00236772241300782

Hasanthi Rathnadiwakara, Sachini Amarasekara, Florence Cliquet, Jean-Christophe Thibault, Roshan N Jalaldeen, Mangala Gunatilake

{"title":"Cellular immune response against rabies vaccination in laboratory-bred dogs: A systematised review.","authors":"Hasanthi Rathnadiwakara, Sachini Amarasekara, Florence Cliquet, Jean-Christophe Thibault, Roshan N Jalaldeen, Mangala Gunatilake","doi":"10.1177/00236772241300782","DOIUrl":"10.1177/00236772241300782","url":null,"abstract":"The immunogenicity of rabies vaccines is commonly measured by serological testing, which includes measuring rabies virus-neutralising antibody titre levels in the serum. Apart from humoral immunity, cellular immunity measurements are also helpful in assessing the immunogenicity and efficacy of rabies vaccinations. Recently, there has been an increased emphasis on cellular immunity measurements against rabies in humans and animals. This review aimed to systematically analyse the literature on the composition of cellular immune responses against rabies vaccination in laboratory dogs. A literature survey was conducted to collect suitable articles by searching the research databases PubMed, Web of Science and Scopus. Subsequently, a two-person screening was conducted to identify suitable articles based on predetermined inclusion and exclusion criteria. Data extraction was performed by two authors independently. A total of 1396 studies were identified from the initial search. Following the screening, six studies were selected for final review. Different methods of detecting immune parameters from peripheral blood mononuclear cells were identified from the studies. Reports have demonstrated positive outcomes of the use of adjuvants for cellular immunity development. Even though the lack of specific immunological techniques and the specific reagents have negatively affected these types of cellular immunity measurements, it was evident that combining both humoral and cellular immune parameters against the rabies antigen would provide a clearer picture of the level of responsiveness in animals towards vaccinations and the protection against the disease. The protocol for this review was published in the International Prospective Register of Systematic Review (CRD42022380023).","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"342-355"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abstracts to the 16th FELASA Congress 2025. 2025年第16届FELASA大会摘要。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-06-02 DOI: 10.1177/00236772251334337

引用次数: 0

Current research and guidelines for euthanasia in laboratory fish with a focus on fathead minnows. 目前对实验鱼的安乐死研究和指导方针，重点是黑头鲦鱼。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-25 DOI: 10.1177/00236772241288146

Christine Lavalle, Stacey Clarence, Hufsa Khan, Kallie Shires, Joanne Parrott

{"title":"Current research and guidelines for euthanasia in laboratory fish with a focus on fathead minnows.","authors":"Christine Lavalle, Stacey Clarence, Hufsa Khan, Kallie Shires, Joanne Parrott","doi":"10.1177/00236772241288146","DOIUrl":"10.1177/00236772241288146","url":null,"abstract":"This paper reviews the methods and approaches used to humanely anesthetize (render unconscious) and or euthanize (kill) laboratory fish (in research settings), with a specific focus on the fathead minnow. We surveyed the literature (333 scientific studies published 2004-2021) to examine euthanasia methods used for various life stages. Our findings showed that many published scientific papers do not provide an adequate description of anesthesia or euthanasia methods, particularly for larval fathead minnows. Over the two decades there was a 20% increase in the number of papers that described their euthanasia method(s). In addition, the review shows evidence that younger minnows require higher concentrations of anesthetic (compared with adults) for effective euthanasia. Recommendations from the review include the use of a two-step euthanasia method (immersion in anesthetic followed by spinal severance and/or exsanguination). As well, it is recommended that details of anesthesia and euthanasia are more fully captured in published scientific manuscripts to allow for comparison among studies and for progress in animal welfare methods. Specific research questions remain on whether rapid cooling is a humane first-step euthanasia method, better investigations into understanding when anesthesia has occurred in fish, and research into methods of euthanasia in larval and juvenile fish.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"319-331"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0