Laboratory Animals最新文献

A framework for assessing practical competence in laboratory animal science (LAS): development of direct observation of procedural skills (DOPS) by the ETPLAS Working Group. 评估实验动物科学（LAS）实践能力的框架：ETPLAS工作组对程序技能（DOPS）直接观察的发展。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-05-06 DOI: 10.1177/00236772261436913

Rafael Frías Beneyto, Ivo Tiebosch, Andreia Costa, Daniel Kylmann Hansen, Åsa Holmberg, Ronald Vlasbom, Lucy Whitfield, Anne-Dominique Degryse, Jan-Bas Prins

{"title":"A framework for assessing practical competence in laboratory animal science (LAS): development of direct observation of procedural skills (DOPS) by the ETPLAS Working Group.","authors":"Rafael Frías Beneyto, Ivo Tiebosch, Andreia Costa, Daniel Kylmann Hansen, Åsa Holmberg, Ronald Vlasbom, Lucy Whitfield, Anne-Dominique Degryse, Jan-Bas Prins","doi":"10.1177/00236772261436913","DOIUrl":"https://doi.org/10.1177/00236772261436913","url":null,"abstract":"Assessment of practical skills is a key component in ensuring competence in laboratory animal science (LAS), particularly for practitioners performing functions A, C and D as defined in the Directive 2010/63/EU, namely those who carry out procedures on animals, care for them and perform humane killing, respectively. To address the need for a harmonised, objective and transparent approach to assessing practical competence, Working Group 4 (WG4) of the Education and Training Platform for Laboratory Animal Science (ETPLAS) has developed a set of Direct Observation of Procedural Skills (DOPS) tools. These tools are tailored specifically to evaluate the intended learning outcomes of Modules 3.2, 6.2, 8 and 20-22 of the European Union (EU) Education and Training Framework. This report outlines the rationale, structure and guidance for implementing the DOPS tools. Based on Miller's pyramid and established educational principles, DOPS tools are suitable for formative and summative assessments in simulated or real environments. They can be used both formatively to monitor progress during training and summatively to support end-of-training decisions about competence. Their implementation is crucial to supporting harmonised training, facilitating mutual recognition of competence and ultimately advancing both animal welfare and the quality of scientific research in the EU and beyond. The DOPS forms are available as supplemental material to this article and from the ETPLAS website.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261436913"},"PeriodicalIF":1.3,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

News from the EIC team. 来自EIC团队的消息。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-05-04 DOI: 10.1177/00236772261445336

Fernando Gonzalez-Uarquin, Paulin Jirkof, Annemarie Lang, Jordi L Tremoleda

引用次数: 0

Caring, killing and an always busy schedule: a cross-sectional study on stress and strain in laboratory animal professionals in German-speaking countries. 照顾、杀戮和总是忙碌的日程：德语国家实验动物专业人员压力和紧张的横断面研究。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-26 DOI: 10.1177/00236772261417391

Sonja Rumpel, Regina Kempen, Roswitha Merle, Christa Thöne-Reineke

{"title":"Caring, killing and an always busy schedule: a cross-sectional study on stress and strain in laboratory animal professionals in German-speaking countries.","authors":"Sonja Rumpel, Regina Kempen, Roswitha Merle, Christa Thöne-Reineke","doi":"10.1177/00236772261417391","DOIUrl":"https://doi.org/10.1177/00236772261417391","url":null,"abstract":"Various studies have documented psychological strain in laboratory animal professionals (LAPs). However, reliable data on relevant stressors remain limited. This study aimed to assess potential stressors experienced by LAPs in German-speaking countries and analyse their impact on psychological strain. An online survey was conducted in summer 2023, assessing the participants' mental strain using the General Health Questionnaire (GHQ-12), common general stressors using the Copenhagen Psychosocial Questionnaire (COPSOQ), as well as potential job-specific stressors. The final sample included 932 anonymous LAP participants. Data were analysed descriptively and using hierarchical regression analysis. Tasks like the euthanasia of surplus animals or being unable to alleviate animal distress due to scientific needs emerged as job-specific stressors. However, the results of the hierarchical regression indicated that general stressors, such as work-privacy conflict, physical and emotional demands, the need to hide emotions, and the degree of one's own influence at work, explained at least as much of the variance in psychological strain as the specific stressors. In addition, work experience, function, and gender significantly influenced the GHQ score. The results from this study could contribute to fostering a culture of care by developing general protection means for the psychological hazards in the workplaces of LAPs, thus reducing their adverse effects on personnel, laboratory animals and research results.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261417391"},"PeriodicalIF":1.3,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing the 3Rs? Researchers' perspectives on institutional facilitation in Switzerland - Part 1: originative facilitation. 推进3r ？瑞士制度便利研究视角——第一部分：创新便利。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-19 DOI: 10.1177/00236772261436225

Edwin Louis-Maerten, Lester D Geneviève, Love Hansell, Charlotte E Blattner, Bernice S Elger

{"title":"Advancing the 3Rs? Researchers' perspectives on institutional facilitation in Switzerland - Part 1: originative facilitation.","authors":"Edwin Louis-Maerten, Lester D Geneviève, Love Hansell, Charlotte E Blattner, Bernice S Elger","doi":"10.1177/00236772261436225","DOIUrl":"https://doi.org/10.1177/00236772261436225","url":null,"abstract":"The implementation of the 3R principles (Replace, Reduce, Refine) requires a comprehensive understanding of the various levels at which they operate, as well as the factors that may hinder or support their advancement. Central to this process is incorporating the perspectives of stakeholders in biomedical research to develop practical and relevant strategies for action. In a qualitative study, we conducted 35 semi-structured interviews with Swiss researchers working on the 3Rs or animal experimentation. Interviews were analysed following a reflexive thematic analysis, and we identified two categories of facilitation: originative facilitation, which concerns epistemic aspects of the 3Rs, and executive facilitation, which concerns interventionist aspects of the 3Rs. In this article, we report on the former dimension, which can be further divided into the conceptualisation of the 3Rs on the one hand, and the education of researchers on the other hand. Although grounded in the Swiss context, these findings reveal key insights for researchers, policymakers, and institutional stakeholders internationally. As efforts to align animal research practices with ethical standards continue to grow globally, understanding how the 3Rs are conceptualised and taught is essential for creating coherent, cross-border strategies that foster responsible scientific practice. Clarifying definitions and reinforcing researcher education are critical steps in ensuring the consistent and meaningful implementation of the 3Rs across national boundaries.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261436225"},"PeriodicalIF":1.3,"publicationDate":"2026-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science. 建立桥梁：动物护理人员和实验室技术人员参与实验计划和动物研究的实施，以提高工作满意度和科学水平。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-14 DOI: 10.1177/00236772251400976

Fernando Gonzalez-Uarquin, Paulin Jirkof, Bettina Bert, Penny Hawkins, Ljupco Angelovski, Jan Baumgart, Nadine Baumgart, Özge S Cevik, Nuno H Franco, Erdal Horata, Rohish Kaura, Winfried Neuhaus, Brigida Riso, Adrian J Smith, Athanassia Sotiropoulos, Augusto Vitale, Sophie Schober

{"title":"Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science.","authors":"Fernando Gonzalez-Uarquin, Paulin Jirkof, Bettina Bert, Penny Hawkins, Ljupco Angelovski, Jan Baumgart, Nadine Baumgart, Özge S Cevik, Nuno H Franco, Erdal Horata, Rohish Kaura, Winfried Neuhaus, Brigida Riso, Adrian J Smith, Athanassia Sotiropoulos, Augusto Vitale, Sophie Schober","doi":"10.1177/00236772251400976","DOIUrl":"https://doi.org/10.1177/00236772251400976","url":null,"abstract":"The involvement of non-scientific staff in discussions about animal welfare and scientific quality is essential for biomedical research progress. In this study, we developed a survey to collect the self-perception of animal care staff (ACS) and laboratory technicians about their involvement in scientific planning and conduct. Participants were contacted to complete an anonymous online questionnaire. We obtained 850 responses, mainly from Europe: 564 from ACS and 286 from laboratory technicians. Job satisfaction was assessed as positive by ACS and laboratory technicians despite the low frequency of culture of care activities and mental health meetings. Both groups expressed their desire to be trained in research planning and conduct; however, regular training was not reported. In addition, the inability to act on animal welfare concerns owing to experimental reasons was reported by both groups. Over half of the participants felt valued and appreciated by the lead scientists or animal facility manager; however, it is not clear how they are acknowledged, as their names on the authors list or in the manuscript acknowledgments are barely included. Our results indicated that involvement of ACS and laboratory technicians in planning and conducting studies would improve their understanding of how experiments are done, and therefore communication processes, work satisfaction, animal welfare, and scientific quality. Finally, we provided recommendations to improve the engagement of ACS and laboratory technicians in discussions about animal research planning and conduct.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251400976"},"PeriodicalIF":1.3,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A house is not a home: Shelter preferences of laboratory mice. 房子不是家：实验室老鼠的庇护偏好。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-08 DOI: 10.1177/00236772251404062

Alina Bonorand, Marie Leekens, Bernhard Voelkl, Hanno Würbel, Janja Novak

{"title":"A house is not a home: Shelter preferences of laboratory mice.","authors":"Alina Bonorand, Marie Leekens, Bernhard Voelkl, Hanno Würbel, Janja Novak","doi":"10.1177/00236772251404062","DOIUrl":"https://doi.org/10.1177/00236772251404062","url":null,"abstract":"The design of many shelters for laboratory mice, particularly plastic shelters, is mainly driven by economic, ergonomic and hygiene considerations, with little focus on providing the animals with opportunities to express species-specific behaviours and control over their environment. Even in the presence of artificial shelters (plastic houses, tunnels), laboratory mice will readily burrow if given the opportunity. The main aim of this study was to gain a better understanding of the importance of access to sheltered space for laboratory mice. We pair-housed mice (BALB/c, SWISS and C57BL/6, male and female) in conventional laboratory cages that contained either (i) resources to build underground shelter (burrowing substrate) or (ii) artificial structures providing shelter (e.g. red plastic house). We assessed how mice used these resources, as well as their preferences when given a choice between different shelters. In addition, we assessed the insulating properties of different shelters. Mice spent most of their time underground in deep bedding and the least time inside the standard red plastic house, where they nested outside the house. They also showed a strong preference for the deep burrow cage, and the most relevant features of preferred shelters seem to be darkness and being underground. Nesting material alone was also less insulating, possibly contributing to an increased need for non-shivering thermogenesis.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251404062"},"PeriodicalIF":1.3,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of adhesion scoring performance between humans and large language models in experimental rat laparotomy. 实验大鼠剖腹手术中人类与大型语言模型粘连评分的比较。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-08 DOI: 10.1177/00236772261426654

Sıtkıcan Okur, Esra Modoğlu, Büşra Baykal, Latif E Yanmaz, Esra M Kirman, Ayşe G Bedir, Damla T Okur, Yasemin Akçora

{"title":"Comparison of adhesion scoring performance between humans and large language models in experimental rat laparotomy.","authors":"Sıtkıcan Okur, Esra Modoğlu, Büşra Baykal, Latif E Yanmaz, Esra M Kirman, Ayşe G Bedir, Damla T Okur, Yasemin Akçora","doi":"10.1177/00236772261426654","DOIUrl":"https://doi.org/10.1177/00236772261426654","url":null,"abstract":"This study compared the macroscopic adhesion scoring performance of large language models (LLMs: ChatGPT-o3, ChatGPT-5, Gemini-2.5 Pro) with that of novice veterinary surgeons, using expert consensus as the reference. Eighty standardized postoperative laparotomy cases in Wistar rats were photographed and scored using the Nair 0-4 adhesion scale. Two novice surgeons and three LLMs independently evaluated each case; the expert reference was defined by a surgeon and a pathologist. Group differences were analyzed using the Kruskal-Wallis test with Dunn-Bonferroni post hoc comparisons, correlations by Bonferroni-adjusted Spearman coefficients, human interobserver reliability by intraclass correlation coefficient (ICC) (A,1), and agreement with the expert by quadratic-weighted Cohen's κ and exact-match accuracy. Overall differences were significant. ChatGPT-o3, ChatGPT-5, Gemini-2.5 Pro, and Novice 1 assigned lower scores, while Novice 2 assigned higher scores. Correlations with the expert were significant for Novice 1 (ρ = 0.706), Novice 2 (ρ = 0.593), and ChatGPT-o3 (ρ = 0.617), but not for ChatGPT-5 or Gemini-2.5 Pro. Inter-observer reliability among human raters was moderate (ICC = 0.55). Importantly, absolute exact-match accuracies were modest across all evaluators, with the highest accuracy observed for Novice 1 (33.8%) and ⩽26.3% for the LLMs. While novices outperformed the models, these findings highlight the intrinsic difficulty of fine-grained Nair 0-4 adhesion scoring on two-dimensional intraoperative images and indicate that current LLMs are better suited as calibrated decision-support tools rather than stand-alone raters.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261426654"},"PeriodicalIF":1.3,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population-genomic stability of a closed-herd GGTA1-knock-out miniature pig line for xenotransplantation. 用于异种移植的封闭群ggta1敲除小型猪系的群体基因组稳定性。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-08 DOI: 10.1177/00236772261432004

Won Kil Lee, Sang Eun Kim, Dongwon Seo, Seunghoon Lee, Jin-Gu No, Seokho Kim, Min Hwa Do, Joo Young Lee, Namwoong Hyeong, Hwi-Cheul Lee, Poongyeon Lee, Keon Bong Oh

{"title":"Population-genomic stability of a closed-herd GGTA1-knock-out miniature pig line for xenotransplantation.","authors":"Won Kil Lee, Sang Eun Kim, Dongwon Seo, Seunghoon Lee, Jin-Gu No, Seokho Kim, Min Hwa Do, Joo Young Lee, Namwoong Hyeong, Hwi-Cheul Lee, Poongyeon Lee, Keon Bong Oh","doi":"10.1177/00236772261432004","DOIUrl":"https://doi.org/10.1177/00236772261432004","url":null,"abstract":"Genetically engineered pigs are essential donors for xenotransplantation, requiring phenotypic stability and genetic definition. We evaluated the newly established GGTA1-knock-out \"XENO\" line, maintained as a closed herd for >10 generations, against Massachusetts General Hospital (MGH) miniature swine, commercial Landrace (LR), and Yorkshire × Landrace (Y × L) populations. One hundred and thirty-nine pigs were genotyped using an 80K SNP BeadChip. Morphometric monitoring at 18 months showed no significant differences in body weight, length, height, or heart girth between XENO and MGH pigs (all p > 0.05). Principal component and phylogenetic analyses separated the four groups into distinct genetic clusters, confirming the uniqueness of the XENO line. Chromosome-wide linkage disequilibrium was markedly higher in XENO (initial r2 > 0.8; half-decay ≈ 50 kb) than in commercial lines, reflecting intensive inbreeding. Linkage disequilibrium-derived historical effective population size (Ne) in XENO was approximately 1.5-fold lower than in LR/Y × L, but comparable to MGH. ADMIXTURE analysis supported K = 3 ancestral components with <2% introgression into XENO. These findings demonstrate that closed-herd management preserves phenotypic uniformity while establishing a genetically homogeneous, independent donor line. XENO pigs exhibited overlapping growth trajectories with MGH animals, providing a genomically stable resource for preclinical xenotransplantation.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261432004"},"PeriodicalIF":1.3,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to teach practical skills with live laboratory animals? Using collective reflection to explore approaches and systematise strategies. 如何教授活体实验动物的实践技能？利用集体反思探索方法和系统化策略。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-03-11 DOI: 10.1177/00236772251407875

Andreia Costa, Júlio Borlido Santos, I Anna S Olsson

{"title":"How to teach practical skills with live laboratory animals? Using collective reflection to explore approaches and systematise strategies.","authors":"Andreia Costa, Júlio Borlido Santos, I Anna S Olsson","doi":"10.1177/00236772251407875","DOIUrl":"https://doi.org/10.1177/00236772251407875","url":null,"abstract":"Researchers need training to develop adequate competence before using animals in experimental procedures. In contrast to medical training, there has been almost no scholarly attention to questions of methodology for teaching practical skills with laboratory animals. In this paper, we explore how the experience of laboratory animal trainers can be combined with existing scholarship on learning and teaching practical skills in related fields. Data were collected through facilitated group discussions in a series of workshops involving a total of 72 participants from 18 countries. Participants identified the 'See one, Do one' method as their preferred teaching approach in practical classes, and recognised the importance of strategies for progressive and adapted learning. They also acknowledged different types of challenging situations, including trainees who have previously developed proficiency with incorrect techniques, trainees who appear uninterested, trainees expressing aversion to animals and trainees who are reluctant to subject animals to harmful procedures. Although participants presented strategies for overcoming the majority of these challenges, they also highlighted specific scenarios where they felt unable to provide adequate support or overcome the challenges within the training environment. This work represents a first systematic scholarly effort to capture and describe teaching strategies used by practical laboratory animal science trainers, an essential step for education and competency development research and practice in the field.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251407875"},"PeriodicalIF":1.3,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147434234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the convergence site as a refinement over the facial vein for chin bleed of CD1 female mice. CD1雌性小鼠颏出血会聚部位的评价。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-03-11 DOI: 10.1177/00236772251411794

Andrea Pardo-Pedraza, Chereen Collymore, Lucia Amendola

引用次数: 0