Laboratory Animals最新文献

Cardiorespiratory, hemodynamic, and sedative effects of dexmedetomidine in sheep. 右美托咪定对绵羊心肺功能、血液动力学和镇静作用的影响。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-11-20 DOI: 10.1177/00236772241277891

Janainne Hilbig Gomes, Isabela Plazza Bittar, Rauane Sousa de Moura, Bruna Ginú Prado, Leandro Guimarães Franco

{"title":"Cardiorespiratory, hemodynamic, and sedative effects of dexmedetomidine in sheep.","authors":"Janainne Hilbig Gomes, Isabela Plazza Bittar, Rauane Sousa de Moura, Bruna Ginú Prado, Leandro Guimarães Franco","doi":"10.1177/00236772241277891","DOIUrl":"https://doi.org/10.1177/00236772241277891","url":null,"abstract":"An alternative in an attempt to minimize the effects triggered by intravenous (IV) bolus administration of α-2 adrenergic receptor agonists are continuous rate infusions (CRI). The requirement for sedation protocols in sheep for procedures to be performed without physical restraint and with reduced adverse effects, commonly observed with bolus use, justifies the study of CRI. The aim of study was to compare the cardiopulmonary and sedative effects of IV bolus injection and CRI of dexmedetomidine (DEX) in sheep. Six adult male sheep (38.3 ± 7.6 kg) received DEX as a bolus (5 µg kg-1, DEXbolus treatment) or CRI (5 µg kg-1 h-1 for 1 h, DEXCRI treatment). We recorded heartrate (HR), respiratory rate, systemic arterial blood pressure, pulse oximetry, hemodynamic parameters, blood gases and sedation scores over 120 min. HR was significantly lower in DEXbolus at 5 and 15 min than in DEXCRI, with HR reduction observed for 30 min in DEXbolus. Hypoxemia was noted in DEXbolus at 10 and 30 min. Pulmonary vascular resistance index increased at 5 min, and cardiac index (CI) decreased at all timepoints compared with baseline in DEXbolus. In DEXCRI, CI decreased only at 45 min. Sedation scores were higher in DEXbolus at 15 and 30 min. DEX CRI administration resulted in fewer cardiorespiratory and hemodynamic changes compared with bolus injection and lower sedation scores (<4/10), which would not allow animal handling without a reaction. The load dose used in CRI was a limitation to constant infusion.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241277891"},"PeriodicalIF":1.3,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of zoonoses in research institutions - lessons learned from a Coxiella burnetii outbreak case. 研究机构的人畜共患病管理--从一例烧伤柯西氏菌爆发中汲取的教训。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-11-19 DOI: 10.1177/00236772241271028

Pia Ks Ostach, André Dülsner, Anne Keil, Stefan Nagel-Riedasch

{"title":"Management of zoonoses in research institutions - lessons learned from a Coxiella burnetii outbreak case.","authors":"Pia Ks Ostach, André Dülsner, Anne Keil, Stefan Nagel-Riedasch","doi":"10.1177/00236772241271028","DOIUrl":"10.1177/00236772241271028","url":null,"abstract":"When biomedical research investigates the human surgical situation in the need of a chronic course, it is more often possible to do so using large animal models. The use of farm animals always poses special challenges for the institution conducting the research in terms of infection prevention and occupational safety. Especially for the zoonotic disease coxiellosis it is important to be aware of the constant risk of pathogen introduction by small ruminants and to take appropriate precautions. In this way, personal injury should be avoided or at least be kept to a minimum in the event of infection since then sustainable zoonosis control can be immediately initiated. Using the example of a Q fever outbreak at a research facility, we want to share with this extended case report the importance of central emergency structures, provisions and the inclusion of relevant experts and disciplines in a crisis team. Its primary purpose is to support the affected facility and coordinate the implementation of necessary cleaning, disinfection and decontamination measures in close contact with the responsible local authorities. The aim is to inactivate the pathogen in a systematic and controlled manner in few steps of action only and to keep the interruption of the facility's operations as short as possible.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241271028"},"PeriodicalIF":1.3,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extended oxygen supplementation after thoracotomy in rats may improve welfare. 大鼠胸廓切开术后延长氧气补充时间可改善福利。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-11-04 DOI: 10.1177/00236772241273061

Bryony Few, Alex Dugdale

{"title":"Extended oxygen supplementation after thoracotomy in rats may improve welfare.","authors":"Bryony Few, Alex Dugdale","doi":"10.1177/00236772241273061","DOIUrl":"https://doi.org/10.1177/00236772241273061","url":null,"abstract":"A retrospective comparison of welfare indicators in male rats undergoing thoracotomy for intrapleural dosing is presented. The initial cohort (n = 7) breathed room air after recovery from anaesthesia, while later cohorts (n = 12) had oxygen supplementation for up to 48 h post-surgery. Rats breathing room air sustained a statistically significant average body-weight loss of -1.62% (±1.7%) 2 days after surgery, compared with rats given oxygen supplementation, which maintained a mean weight gain of 0.87% (±1.75%) (p = 0.009). Oxygen-supplemented rats also had lower pain scores on the evening after surgery (median 0.075 [range 0-1.75] vs. median 1.5 [range 0.5-2]). This difference was not statistically significant (p = 0.063) but may be of clinical significance. All rats displayed transient sedation after post-operative opioid administration on the day of surgery, and hypoxaemia (SpO2 <90%) was observed in rats maintained on room air. Use of an oxygen concentrator to provide post-operative extended oxygen supplementation was easy to implement and may improve animal welfare post-thoracotomy.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241273061"},"PeriodicalIF":1.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An innovative approach for health and safety training and occupational health program annual enrollment for laboratory animal care and use personnel. 为实验室动物护理和使用人员提供健康与安全培训和职业健康计划年度注册的创新方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-11-04 DOI: 10.1177/00236772241263903

Scott J Patlovich, Robert J Emery, Kristin G King, Zackary B Becker, Rebecca B Kairis, Mary A Robinson, Joy M Harrison, George Delclos, William B Perkison

{"title":"An innovative approach for health and safety training and occupational health program annual enrollment for laboratory animal care and use personnel.","authors":"Scott J Patlovich, Robert J Emery, Kristin G King, Zackary B Becker, Rebecca B Kairis, Mary A Robinson, Joy M Harrison, George Delclos, William B Perkison","doi":"10.1177/00236772241263903","DOIUrl":"https://doi.org/10.1177/00236772241263903","url":null,"abstract":"Animal care and use personnel in research laboratory facilities are inherently exposed to a variety of workplace hazards. The health and safety of the workforce working directly with or around research animals is of paramount importance, and as such, an occupational health and safety program for at-risk staff is essential. In order to maximize participation in and the effectiveness of health and safety training and occupational health program enrollment for animal care and use personnel at an academic health sciences university, an innovative annual \"health fair\" was developed and implemented at The University of Texas Health Science Center at Houston. This event allows personnel working in positions that present exposure risk to research animals to be able to obtain hazard-specific health and safety training, re-enroll in the occupational health oversight program and update their medical history, and participate in the institution's other safety programs as required by job title or assigned job tasks. By providing a comprehensive health fair that offers convenient access to the necessary safety training and occupational health services in one location during a designated period of time, management and staff are incentivized to participate and have reported satisfaction with and appreciation of the convenient access. Summarized here is how we plan, organize, and effectively execute the annual health fair so that other institutions who might wish to use this strategy can learn from our approach.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241263903"},"PeriodicalIF":1.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Animal researchers' views on the publication of negative results and subsequent policy adoptions. 动物研究人员对公布负面结果的看法以及随后采取的政策。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-24 DOI: 10.1177/00236772241271039

Carlos Oscar S Sorzano, Daniel G Hamilton, Eva Mendez

引用次数: 0

Spontaneous hepatocellular and gastric tumours in African clawed frogs (Xenopus laevis). 非洲爪蛙（Xenopus laevis）的自发性肝细胞瘤和胃瘤。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-22 DOI: 10.1177/00236772241273060

Bernat Martí-García, Simon L Priestnall, Yolanda Saavedra Torres, Alejandro Suárez-Bonnet

{"title":"Spontaneous hepatocellular and gastric tumours in African clawed frogs (Xenopus laevis).","authors":"Bernat Martí-García, Simon L Priestnall, Yolanda Saavedra Torres, Alejandro Suárez-Bonnet","doi":"10.1177/00236772241273060","DOIUrl":"https://doi.org/10.1177/00236772241273060","url":null,"abstract":"Three seven-year-old African clawed frogs (Xenopus laevis) from an animal research facility showed weight loss, coelomic distention and an abnormal swimming gait were euthanised on welfare grounds. A complete necropsy of each animal showed a focal, firm, dark brown to green mass with multifocal haemorrhages in the left liver lobe in two animals and a transmural, firm, beige, multilobulated gastric mass in another animal. Additionally, one of the frogs with the hepatic mass had haemocoeloma. Histologically, the hepatic masses were diagnosed as hepatocellular adenomas and the gastric mass as a gastric carcinoma with trans-coelomic metastases. The three tumours were immunophenotyped using the following antibodies: cytokeratin AE1/AE3, vimentin, E-cadherin, P53 and Ki67 and, additionally, for the hepatic tumours only, synaptophysin, Prox-1, S100 and Sox-9. Masson's trichrome, Periodic acid Schiff and Gram stains was also performed in selected cases. One of the hepatocellular adenomas was weakly positively labelled with E-cadherin whereas the other showed variable positivity for Sox-9 only. Vimentin labelled the stroma and sinusoidal endothelia. Interestingly in the liver, the cytokeratin AE1/AE3 labelling was restricted to the biliary epithelium and sinusoidal endothelia. The gastric carcinoma labelled positively with cytokeratin AE1/AE3 only. This report aims to guide laboratory animal veterinarians to accurately diagnose multi-organ masses in amphibians. To the authors' knowledge, this is the first comprehensive morphological study on a case series of hepatocellular and gastric neoplasia in X. laevis.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241273060"},"PeriodicalIF":1.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single nucleotide polymorphism (SNP) characterisation of mouse inbred strains bred at MRC-National Institute for Medical Research. 英国国家医学研究所（MRC-National Institute for Medical Research）培育的小鼠近交系的单核苷酸多态性（SNP）特征。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-13 DOI: 10.1177/00236772241273070

José M Sánchez-Morgado

{"title":"Single nucleotide polymorphism (SNP) characterisation of mouse inbred strains bred at MRC-National Institute for Medical Research.","authors":"José M Sánchez-Morgado","doi":"10.1177/00236772241273070","DOIUrl":"https://doi.org/10.1177/00236772241273070","url":null,"abstract":"Inbred mouse strains have long proved useful as tools for biomedical research. They remove the effects of genetic background as an experimental variable. Within all mouse colonies, genetic drift is a recognised phenomenon and monitoring and documenting changes is important for experimental design and consistency. This communication documents the initial characterisation through SNP analysis of the inbred mouse strains bred and used at the time at the Medical Research Council National Institute for Medical Research (MRC-NIMR), Mill Hill, now The Crick Institute. These inbred strains were part of the foundation colonies for the many genetically modified mouse strains made at Mill Hill. We found small genetic changes in four of the nine inbred strains. Although phenotypic differences have not yet been found between the NIMR and the correspondent parental strains, I cannot discard that these may arise or have already arisen. This work has also authenticated the 129/SvJEvNimr-Gpi1c strain that was widely used at MRC-NIMR for gene targeting. All these inbred strains have been renamed according to The International Committee on Standardized Genetic Nomenclature for Mice.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241273070"},"PeriodicalIF":1.3,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of inferior vena cava puncture under continuous cardiac perfusion with cardiac puncture in blood acquisition of the laboratory mouse. 比较在持续心脏灌注下进行下腔静脉穿刺和在实验室小鼠血液采集中进行心脏穿刺。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-11 DOI: 10.1177/00236772241256023

Wengang Hu, Hao Sheng, JiaCai Yang, Cheng Chen, Ruoyu Shang, Zhihui Liu, Xiaohong Hu, Xiaorong Zhang, Weifeng He, Chibing Huang, Gaoxing Luo

{"title":"Comparison of inferior vena cava puncture under continuous cardiac perfusion with cardiac puncture in blood acquisition of the laboratory mouse.","authors":"Wengang Hu, Hao Sheng, JiaCai Yang, Cheng Chen, Ruoyu Shang, Zhihui Liu, Xiaohong Hu, Xiaorong Zhang, Weifeng He, Chibing Huang, Gaoxing Luo","doi":"10.1177/00236772241256023","DOIUrl":"https://doi.org/10.1177/00236772241256023","url":null,"abstract":"Obtaining sufficient blood volume from mice significantly facilitates experimental research. This study explored the inferior vena cava puncture under continuous cardiac perfusion (IVCP-UCCP) technique and evaluated its efficiency in comparison with conventional cardiac puncture (CP). In an initial dose-finding study, 50 mice were randomly assigned to one of 10 groups with escalating perfusion volume from 0.5 to 4.5 ml in 0.5-ml increments. The minimum perfusion volume was determined to be 2 ml in collecting whole circulating blood. In the next comparison using the conventional method, 40 mice were randomly assigned to one of two groups denoting different blood collection methods: Group 1: CP, Group 2: IVCP-UCCP. The results showed 1) that the cells and undiluted blood volume collected via IVCP-UCCP was over twofold higher than that by CP (p < 0.001), confirmed by the cell counts and hematoxylin-eosin staining of different tissues slides (p < 0.001); 2) the new technique did not alter the cellular composition or viability, which was verified by routine blood tests and flow cytometry (p > 0.05); 3) the blood collected via the novel technique was diluted 2.1 times: the hemato-biochemical indicator results multiplied by 2.1 were identical with the test results of blood from CP (p > 0.05). Together, the refined blood collection method of IVCP-UCCP completely extracted the limited blood resources in mice, significantly enhanced the utilization of each mouse, and thus offered scientific and ethical benefits. This technique may be also applicable for other small animal models.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241256023"},"PeriodicalIF":1.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a center for automated equipment processing to transform the research enterprise configuration. 建立自动化设备处理中心，改造科研企业配置。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-11 DOI: 10.1177/00236772241262827

Philip J Richter, Albert LeClair, Harry Lim

{"title":"Development of a center for automated equipment processing to transform the research enterprise configuration.","authors":"Philip J Richter, Albert LeClair, Harry Lim","doi":"10.1177/00236772241262827","DOIUrl":"https://doi.org/10.1177/00236772241262827","url":null,"abstract":"The strategy of centralizing equipment sanitation and processing was developed by a top-tier public university to address the growing physical infrastructure and human resource challenges of its expanding in-vivo research enterprise. Subsequently, a center for automated equipment processing physically separate from all animal research facilities was developed and has operated consistently since 2016. The facility incorporates systems such as process automation to sanitize and sterilize equipment as efficiently as possible. Analysis of the differences between the new centralized and old distributed research enterprise configurations shows a total estimated fiscal benefit of almost US$54 m to date projected out to US$124 m through 2028. Utility consumption of operations over nine years in the new configuration was estimated to be decreased by over 125 million gallons of water, 14 million gallons of chilled water, 121 million pounds of steam, and almost 3.6 million kilowatts of electricity, with consumption savings significantly increased projected out to 2028. Additional operational and organizational benefits as well as direct research benefits were identified. The new configuration has functioned for nine years without detectable cross contamination within the research enterprise thus providing evidence that the location of equipment processing can be less of a \"microbial\" risk factor than previously attributed.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241262827"},"PeriodicalIF":1.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decision trees for determining the fate of laboratory animals. 决定实验动物命运的决策树。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-08 DOI: 10.1177/00236772241271034

Myhailo Reshetnykov, Karen Stumm, Michael Zwick, Jan Baumgart

{"title":"Decision trees for determining the fate of laboratory animals.","authors":"Myhailo Reshetnykov, Karen Stumm, Michael Zwick, Jan Baumgart","doi":"10.1177/00236772241271034","DOIUrl":"https://doi.org/10.1177/00236772241271034","url":null,"abstract":"Facilities involved in laboratory animal research often face ethical challenges such as: what should I do with the animals that are no longer suitable for experimental purposes? One of the common answers to this question is to kill them. And while numerous scientifically justifiable reasons exist for killing laboratory animals, we must not overlook our ethical responsibility towards these sentient beings. Animal facility managers and scientists frequently find themselves in a moral dilemma, torn between furthering their research and addressing the well-being of experimental animals required for their studies. We elaborated a concept consisting of six decision trees and recommendations for making informed decisions about the need to kill laboratory animals in research facilities, considering legal and ethical considerations. The concept is based on the German regulatory perspective. However, the measures and decisions for animal welfare can be implemented in all laboratory animal facilities. These recommendations suggest several courses of action, including implementing consistent breeding plans, exploring alternative uses, reassigning surplus animals and their organs, and establishing appropriate housing capacity limits that ensure species-appropriate care. We encourage scientists and animal facility managers to develop and implement decision-making frameworks and procedures tailored to their specific facilities, in the hope that this work will promote a thoughtful and responsible approach to the complex challenges associated with the killing of laboratory animals, advancing scientific progress and the humane treatment of these animals.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241271034"},"PeriodicalIF":1.3,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0