Laboratory Animals最新文献

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-05-30 DOI: 10.1177/00236772251338015

Annemarie Lang, Paulin Jirkof, Josè Sanchez-Morgado, Jordi L Tremoleda

引用次数: 0

Cellular immune response against rabies vaccination in laboratory-bred dogs: A systematised review. 细胞免疫反应对抗狂犬病疫苗在实验室培育的狗：一个系统的回顾。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-25 DOI: 10.1177/00236772241300782

Hasanthi Rathnadiwakara, Sachini Amarasekara, Florence Cliquet, Jean-Christophe Thibault, Roshan N Jalaldeen, Mangala Gunatilake

{"title":"Cellular immune response against rabies vaccination in laboratory-bred dogs: A systematised review.","authors":"Hasanthi Rathnadiwakara, Sachini Amarasekara, Florence Cliquet, Jean-Christophe Thibault, Roshan N Jalaldeen, Mangala Gunatilake","doi":"10.1177/00236772241300782","DOIUrl":"10.1177/00236772241300782","url":null,"abstract":"The immunogenicity of rabies vaccines is commonly measured by serological testing, which includes measuring rabies virus-neutralising antibody titre levels in the serum. Apart from humoral immunity, cellular immunity measurements are also helpful in assessing the immunogenicity and efficacy of rabies vaccinations. Recently, there has been an increased emphasis on cellular immunity measurements against rabies in humans and animals. This review aimed to systematically analyse the literature on the composition of cellular immune responses against rabies vaccination in laboratory dogs. A literature survey was conducted to collect suitable articles by searching the research databases PubMed, Web of Science and Scopus. Subsequently, a two-person screening was conducted to identify suitable articles based on predetermined inclusion and exclusion criteria. Data extraction was performed by two authors independently. A total of 1396 studies were identified from the initial search. Following the screening, six studies were selected for final review. Different methods of detecting immune parameters from peripheral blood mononuclear cells were identified from the studies. Reports have demonstrated positive outcomes of the use of adjuvants for cellular immunity development. Even though the lack of specific immunological techniques and the specific reagents have negatively affected these types of cellular immunity measurements, it was evident that combining both humoral and cellular immune parameters against the rabies antigen would provide a clearer picture of the level of responsiveness in animals towards vaccinations and the protection against the disease. The protocol for this review was published in the International Prospective Register of Systematic Review (CRD42022380023).","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"342-355"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current research and guidelines for euthanasia in laboratory fish with a focus on fathead minnows. 目前对实验鱼的安乐死研究和指导方针，重点是黑头鲦鱼。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-25 DOI: 10.1177/00236772241288146

Christine Lavalle, Stacey Clarence, Hufsa Khan, Kallie Shires, Joanne Parrott

{"title":"Current research and guidelines for euthanasia in laboratory fish with a focus on fathead minnows.","authors":"Christine Lavalle, Stacey Clarence, Hufsa Khan, Kallie Shires, Joanne Parrott","doi":"10.1177/00236772241288146","DOIUrl":"10.1177/00236772241288146","url":null,"abstract":"This paper reviews the methods and approaches used to humanely anesthetize (render unconscious) and or euthanize (kill) laboratory fish (in research settings), with a specific focus on the fathead minnow. We surveyed the literature (333 scientific studies published 2004-2021) to examine euthanasia methods used for various life stages. Our findings showed that many published scientific papers do not provide an adequate description of anesthesia or euthanasia methods, particularly for larval fathead minnows. Over the two decades there was a 20% increase in the number of papers that described their euthanasia method(s). In addition, the review shows evidence that younger minnows require higher concentrations of anesthetic (compared with adults) for effective euthanasia. Recommendations from the review include the use of a two-step euthanasia method (immersion in anesthetic followed by spinal severance and/or exsanguination). As well, it is recommended that details of anesthesia and euthanasia are more fully captured in published scientific manuscripts to allow for comparison among studies and for progress in animal welfare methods. Specific research questions remain on whether rapid cooling is a humane first-step euthanasia method, better investigations into understanding when anesthesia has occurred in fish, and research into methods of euthanasia in larval and juvenile fish.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"319-331"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective descriptive report comparing access systems to refine intravenous self-administration in rats. 回顾性描述性报告，比较获取系统以改进大鼠静脉自我给药。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-23 DOI: 10.1177/00236772241297777

José G Lozeman-van 't Klooster, Heidi Mb Lesscher

引用次数: 0

Current practices of pain assessment and analgesic use in laboratory mice: A 2022 FELASA Working Group survey. 实验室小鼠疼痛评估和止痛药使用的当前实践：2022年FELASA工作组调查。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-29 DOI: 10.1177/00236772241300779

Ignacio A Gomez de Segura, Saskia Seeldrayers, Paul Flecknell

{"title":"Current practices of pain assessment and analgesic use in laboratory mice: A 2022 FELASA Working Group survey.","authors":"Ignacio A Gomez de Segura, Saskia Seeldrayers, Paul Flecknell","doi":"10.1177/00236772241300779","DOIUrl":"10.1177/00236772241300779","url":null,"abstract":"Assessing and alleviating pain in animals involved in research is critically important. However, the effective implementation of pain management depends on the knowledge and attitudes of the personnel involved. Following a Federation of European Laboratory Animal Science Associations 'Pain in Mice' working group initiative, a questionnaire to survey current practices concerning analgesic use in laboratory mice was distributed to several professional groups in the field of laboratory animal science. Besides demographic data, attitudes to pain and analgesia and sources of information and advice on pain management were assessed. Data were gathered and analysed through an e-survey provider. Most respondents (N = 222) were from Europe (90%). Analgesics were administered to murine surgical models by 92% of respondents in most cases and by 66% to all mice undergoing surgery. Most respondents used multimodal analgesic regimens (69%). For non-surgical models, 34% of respondents provided analgesics. The most commonly administered classes of analgesics were opioids (mostly buprenorphine) and non-steroidal anti-inflammatory drugs (mostly meloxicam and carprofen). A wide range of dose rates of meloxicam and carprofen was reported. Local anaesthetics were also widely used in surgical models (mostly lidocaine). Pain assessment was undertaken by most respondents (98%). In conclusion, most respondents provided analgesics to mice undergoing surgery and used analgesics in some non-surgical models. A considerable variation in the dose range used and the timing of administration of analgesics likely reflects both a lack of data and variation in pain assessment methodologies.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"396-412"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive review of animal models in diabetes research using chemical agents. 利用化学试剂研究糖尿病动物模型的综合综述。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-16 DOI: 10.1177/00236772241296199

Sermin Algul, Oguz Ozcelik

{"title":"Comprehensive review of animal models in diabetes research using chemical agents.","authors":"Sermin Algul, Oguz Ozcelik","doi":"10.1177/00236772241296199","DOIUrl":"10.1177/00236772241296199","url":null,"abstract":"Diabetes mellitus, characterized by insufficient insulin secretion and impaired insulin efficacy, disrupts carbohydrate, protein, and lipid metabolism. The global diabetic population is expected to double by 2025, from 380 million, posing a significant health challenge. Most diabetic individuals fall into the type 1 or type 2 categories, and diabetes adversely affects various organs, such as the kidneys, liver, nervous system, reproductive system, and eyes.This review focuses on animal models of diabetes induced by chemical agents, which are essential tools for understanding disease mechanisms, investigating complications, and testing antidiabetic drugs. Models include those caused by streptozotocin (STZ), alloxan, ferric nitrilotriacetate (Fe-NTA), dithizone, and anti-insulin serum.Streptozotocin (STZ)-induced diabetes models create type 1 and 2 diabetes by destroying pancreatic beta cells. The combination of STZ with nicotinamide mimics type 2 diabetes phenotypes. Alloxan induces a hyperglycemic state by causing free radical formation that selectively destroys pancreatic beta cells. Fe-NTA and dithizone also create diabetes models by damaging pancreatic beta cells. Anti-insulin serum models induce insulin resistance and hyperglycemia by generating antibodies against insulin receptors, leading to a condition similar to type 1 diabetes.Each model has unique characteristics that make it suitable for different aspects of diabetes research. These models are used to understand the pathogenesis of diabetes, develop new treatment strategies, and evaluate the efficacy of potential drugs.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"356-363"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The advisory body on animal ethics of a global pharmaceutical company: A global organization to address ethical and societal concerns about animal use. 全球制药公司动物伦理咨询机构：一个全球性组织，解决有关动物使用的伦理和社会问题。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-23 DOI: 10.1177/00236772241302537

Juliette Schmitt-Lemaître, Nicolas Dudoignon, Edith Stuyven, Thierry Decelle

引用次数: 0

Biological adaptation to a germ-free environment should not be mistaken as a burden for animals. 对无菌环境的生物适应不应被误认为是动物的负担。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-16 DOI: 10.1177/00236772241287847

Silvia Bolsega, André Bleich, Martina Dorsch, Marijana Basic

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Oestrous cycle synchronization protocols in rats using cloprostenol and progesterone injections. 使用氯前列醇和黄体酮注射的大鼠的发情周期同步方案。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-02-13 DOI: 10.1177/00236772241309754

Sophia Potapova, Viktor Stratilov, Oleg Vetrovoy, Diana Safarova, Ekaterina Tyulkova

引用次数: 0

Case report: C57BL/6NTac and C57BL/6NCrl mice displaying neurological signs after deworming with ivermectin. 病例报告：C57BL/6NTac和C57BL/6NCrl小鼠在伊维菌素驱虫后出现神经症状。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2024-12-24 DOI: 10.1177/00236772241286214

M Eriksson, S Nylén

{"title":"Case report: C57BL/6NTac and C57BL/6NCrl mice displaying neurological signs after deworming with ivermectin.","authors":"M Eriksson, S Nylén","doi":"10.1177/00236772241286214","DOIUrl":"10.1177/00236772241286214","url":null,"abstract":"For over 40 years, ivermectin has served as an effective anti-parasitic drug used in human and veterinary medicine. In laboratory animal facilities it is used prophylactically or therapeutically to maintain the health status of the colony or experimentally in studies. Although ivermectin is generally safe to use, there are reports of neurotoxicity associated with ivermectin crossing the blood-brain barrier due to overdosing or blood-brain barrier dysfunction. In mice, P-glycoprotein maintains the blood-brain barrier and mice with a mutation in the P-glycoprotein encoding gene mdr1a are 50-100 times more sensitive to ivermectin. Signs of neurotoxicity include ataxia, bradypnea, recumbency, tremor, and death. We report neurotoxicity after ivermectin administration was used for the purpose of eradicating the murine-specific intestinal nematode Heligmosomoides polygyrus in C57BL/6NTac and C57BL/6NCrl mice. The mice were dewormed by subcutaneous administration of 10 or 20 mg/kg ivermectin to eradicate all stages of Heligmosomoides polygyrus. At 24-48h after deworming, 5% (n = 4) of the mice presented with tremor, ataxia, and/or head tilt. The affected mice were euthanised and gross pathological findings were found in one of the four mice (left-sided hydronephrosis). We assume that the observed neurological effects were due to defects in the blood-brain barrier, overdosing or individual sensitivity. This report provides a reason for caution when deworming laboratory mice subcutaneously with ivermectin at doses of 10 mg/kg or higher.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"374-383"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0