Laboratory Animals最新文献

Murine chapparvovirus (MuCPV)/mouse kidney parvovirus (MKPV) eradication by cross foster rederivation.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-02-04 DOI: 10.1177/00236772241277591

Ibrahim Faseeh, Vatthana Vongphakham, Patrick Sharp

{"title":"Murine chapparvovirus (MuCPV)/mouse kidney parvovirus (MKPV) eradication by cross foster rederivation.","authors":"Ibrahim Faseeh, Vatthana Vongphakham, Patrick Sharp","doi":"10.1177/00236772241277591","DOIUrl":"https://doi.org/10.1177/00236772241277591","url":null,"abstract":"When diagnostic tests became available for the detection of mouse kidney parvovirus (MKPV), also known as murine chapparvovirus (MuCPV), we undertook a facility-wide screening to determine the prevalence of this novel agent in the Animal Resources Centre. MKPV was present only in the Customs Strains, specific-pathogen-free (SPF) barrier area, which was the only animal holding area that received mice imports directly from non-approved vendors. Based on this information, the knowledge and understanding of the viral epizootiology, and the testing methods available, we decided to eradicate MKPV after consultation with the researchers impacted. To eradicate MKPV from the barrier, we took a multi-modal approach composed of testing and separating positive and negative cages; and cross-foster (XF) rederivation. The test and separate approach was unsuccessful due to the animal housing and husbandry set up in the SPF barrier area given that MKPV is an environmentally stable and highly infectious agent. However, with an XF rederivation method, we were able to successfully rederive 11 out of 16 litters of various genetically modified lines that remained MKPV negative over a 20-week testing period. Our trial indicates that XF rederivation techniques, coupled with strict disinfection, can be used for a growing list of viral and other infectious agents that are highly infectious and persistent in the environment.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241277591"},"PeriodicalIF":1.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current practices of pain assessment and analgesic use in laboratory mice: A 2022 FELASA Working Group survey.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-01-29 DOI: 10.1177/00236772241300779

Ignacio A Gomez de Segura, Saskia Seeldrayers, Paul Flecknell

{"title":"Current practices of pain assessment and analgesic use in laboratory mice: A 2022 FELASA Working Group survey.","authors":"Ignacio A Gomez de Segura, Saskia Seeldrayers, Paul Flecknell","doi":"10.1177/00236772241300779","DOIUrl":"https://doi.org/10.1177/00236772241300779","url":null,"abstract":"Assessing and alleviating pain in animals involved in research is critically important. However, the effective implementation of pain management depends on the knowledge and attitudes of the personnel involved. Following a Federation of European Laboratory Animal Science Associations 'Pain in Mice' working group initiative, a questionnaire to survey current practices concerning analgesic use in laboratory mice was distributed to several professional groups in the field of laboratory animal science. Besides demographic data, attitudes to pain and analgesia and sources of information and advice on pain management were assessed. Data were gathered and analysed through an e-survey provider. Most respondents (N = 222) were from Europe (90%). Analgesics were administered to murine surgical models by 92% of respondents in most cases and by 66% to all mice undergoing surgery. Most respondents used multimodal analgesic regimens (69%). For non-surgical models, 34% of respondents provided analgesics. The most commonly administered classes of analgesics were opioids (mostly buprenorphine) and non-steroidal anti-inflammatory drugs (mostly meloxicam and carprofen). A wide range of dose rates of meloxicam and carprofen was reported. Local anaesthetics were also widely used in surgical models (mostly lidocaine). Pain assessment was undertaken by most respondents (98%). In conclusion, most respondents provided analgesics to mice undergoing surgery and used analgesics in some non-surgical models. A considerable variation in the dose range used and the timing of administration of analgesics likely reflects both a lack of data and variation in pain assessment methodologies.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241300779"},"PeriodicalIF":1.3,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular immune response against rabies vaccination in laboratory-bred dogs: A systematised review.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-01-25 DOI: 10.1177/00236772241300782

Hasanthi Rathnadiwakara, Sachini Amarasekara, Florence Cliquet, Jean-Christophe Thibault, Roshan N Jalaldeen, Mangala Gunatilake

{"title":"Cellular immune response against rabies vaccination in laboratory-bred dogs: A systematised review.","authors":"Hasanthi Rathnadiwakara, Sachini Amarasekara, Florence Cliquet, Jean-Christophe Thibault, Roshan N Jalaldeen, Mangala Gunatilake","doi":"10.1177/00236772241300782","DOIUrl":"https://doi.org/10.1177/00236772241300782","url":null,"abstract":"The immunogenicity of rabies vaccines is commonly measured by serological testing, which includes measuring rabies virus-neutralising antibody titre levels in the serum. Apart from humoral immunity, cellular immunity measurements are also helpful in assessing the immunogenicity and efficacy of rabies vaccinations. Recently, there has been an increased emphasis on cellular immunity measurements against rabies in humans and animals. This review aimed to systematically analyse the literature on the composition of cellular immune responses against rabies vaccination in laboratory dogs. A literature survey was conducted to collect suitable articles by searching the research databases PubMed, Web of Science and Scopus. Subsequently, a two-person screening was conducted to identify suitable articles based on predetermined inclusion and exclusion criteria. Data extraction was performed by two authors independently. A total of 1396 studies were identified from the initial search. Following the screening, six studies were selected for final review. Different methods of detecting immune parameters from peripheral blood mononuclear cells were identified from the studies. Reports have demonstrated positive outcomes of the use of adjuvants for cellular immunity development. Even though the lack of specific immunological techniques and the specific reagents have negatively affected these types of cellular immunity measurements, it was evident that combining both humoral and cellular immune parameters against the rabies antigen would provide a clearer picture of the level of responsiveness in animals towards vaccinations and the protection against the disease. The protocol for this review was published in the International Prospective Register of Systematic Review (CRD42022380023).","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241300782"},"PeriodicalIF":1.3,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current research and guidelines for euthanasia in laboratory fish with a focus on fathead minnows.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-01-25 DOI: 10.1177/00236772241288146

Christine Lavalle, Stacey Clarence, Hufsa Khan, Kallie Shires, Joanne Parrott

{"title":"Current research and guidelines for euthanasia in laboratory fish with a focus on fathead minnows.","authors":"Christine Lavalle, Stacey Clarence, Hufsa Khan, Kallie Shires, Joanne Parrott","doi":"10.1177/00236772241288146","DOIUrl":"https://doi.org/10.1177/00236772241288146","url":null,"abstract":"This paper reviews the methods and approaches used to humanely anesthetize (render unconscious) and or euthanize (kill) laboratory fish (in research settings), with a specific focus on the fathead minnow. We surveyed the literature (333 scientific studies published 2004-2021) to examine euthanasia methods used for various life stages. Our findings showed that many published scientific papers do not provide an adequate description of anesthesia or euthanasia methods, particularly for larval fathead minnows. Over the two decades there was a 20% increase in the number of papers that described their euthanasia method(s). In addition, the review shows evidence that younger minnows require higher concentrations of anesthetic (compared with adults) for effective euthanasia. Recommendations from the review include the use of a two-step euthanasia method (immersion in anesthetic followed by spinal severance and/or exsanguination). As well, it is recommended that details of anesthesia and euthanasia are more fully captured in published scientific manuscripts to allow for comparison among studies and for progress in animal welfare methods. Specific research questions remain on whether rapid cooling is a humane first-step euthanasia method, better investigations into understanding when anesthesia has occurred in fish, and research into methods of euthanasia in larval and juvenile fish.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241288146"},"PeriodicalIF":1.3,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inconsistent terminology in rodent anatomy hinders appropriate blood sampling in laboratory animal science: A review of blood sampling techniques on the head of mice.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-01-25 DOI: 10.1177/00236772241304504

Malan Štrbenc

引用次数: 0

Moving from guideline recommendations to practical, reasonable proposals: Biosafety considerations, breeding performance and challenges during specific pathogen-free animal facility enlargement.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-01-24 DOI: 10.1177/00236772241301123

Maschi Fabricio, Laborde Juan, Carriquiriborde Martín, Milocco Silvana, Almirón Johana, Resasco Agustina, Ayala Miguel

{"title":"Moving from guideline recommendations to practical, reasonable proposals: Biosafety considerations, breeding performance and challenges during specific pathogen-free animal facility enlargement.","authors":"Maschi Fabricio, Laborde Juan, Carriquiriborde Martín, Milocco Silvana, Almirón Johana, Resasco Agustina, Ayala Miguel","doi":"10.1177/00236772241301123","DOIUrl":"https://doi.org/10.1177/00236772241301123","url":null,"abstract":"Extending an existing animal facility is a challenging process that requires consideration of both engineering and biological aspects. In this sense, integration with ongoing activities must not alter the animals' microbiological condition or welfare, as they usually remain in the facility while these activities occur. The objective of this work was to describe and evaluate the practical biosafety considerations during the enlargement of a specific pathogen-free (SPF) rodent facility. Our facility breeds rats and mice free of a list of zoonotic and common rodent pathogens, comprising 6 ectoparasites, 13 endoparasites, 25 bacteria and 23 viruses. In this project, the new SPF area was connected to an old but still working SPF rodent facility through the original clean corridor. The old clean corridor remained sealed throughout the project, and it was not opened until the new area was finished and fully equipped, all the new rooms were cleaned and disinfected, and the environment was evaluated for the presence of pathogens. Timing during the project was essential, as avoidance of the period of high animal production and demand was sought. The microbiological controls showed no growth of microorganisms in any new room. Thus, the applied procedures were deemed effective. It was concluded that protocols should be carefully planned in order to maintain the SPF condition and animal welfare.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241301123"},"PeriodicalIF":1.3,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective descriptive report comparing access systems to refine intravenous self-administration in rats.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-01-23 DOI: 10.1177/00236772241297777

José G Lozeman-van 't Klooster, Heidi Mb Lesscher

引用次数: 0

The advisory body on animal ethics of a global pharmaceutical company: A global organization to address ethical and societal concerns about animal use.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-01-23 DOI: 10.1177/00236772241302537

Juliette Schmitt-Lemaître, Nicolas Dudoignon, Edith Stuyven, Thierry Decelle

引用次数: 0

Comprehensive review of animal models in diabetes research using chemical agents. 利用化学试剂研究糖尿病动物模型的综合综述。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-01-16 DOI: 10.1177/00236772241296199

Sermin Algul, Oguz Ozcelik

{"title":"Comprehensive review of animal models in diabetes research using chemical agents.","authors":"Sermin Algul, Oguz Ozcelik","doi":"10.1177/00236772241296199","DOIUrl":"https://doi.org/10.1177/00236772241296199","url":null,"abstract":"Diabetes mellitus, characterized by insufficient insulin secretion and impaired insulin efficacy, disrupts carbohydrate, protein, and lipid metabolism. The global diabetic population is expected to double by 2025, from 380 million, posing a significant health challenge. Most diabetic individuals fall into the type 1 or type 2 categories, and diabetes adversely affects various organs, such as the kidneys, liver, nervous system, reproductive system, and eyes.This review focuses on animal models of diabetes induced by chemical agents, which are essential tools for understanding disease mechanisms, investigating complications, and testing antidiabetic drugs. Models include those caused by streptozotocin (STZ), alloxan, ferric nitrilotriacetate (Fe-NTA), dithizone, and anti-insulin serum.Streptozotocin (STZ)-induced diabetes models create type 1 and 2 diabetes by destroying pancreatic beta cells. The combination of STZ with nicotinamide mimics type 2 diabetes phenotypes. Alloxan induces a hyperglycemic state by causing free radical formation that selectively destroys pancreatic beta cells. Fe-NTA and dithizone also create diabetes models by damaging pancreatic beta cells. Anti-insulin serum models induce insulin resistance and hyperglycemia by generating antibodies against insulin receptors, leading to a condition similar to type 1 diabetes.Each model has unique characteristics that make it suitable for different aspects of diabetes research. These models are used to understand the pathogenesis of diabetes, develop new treatment strategies, and evaluate the efficacy of potential drugs.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241296199"},"PeriodicalIF":1.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological adaptation to a germ-free environment should not be mistaken as a burden for animals. 对无菌环境的生物适应不应被误认为是动物的负担。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-01-16 DOI: 10.1177/00236772241287847

Silvia Bolsega, André Bleich, Martina Dorsch, Marijana Basic

引用次数: 0