Laboratory Animals最新文献

The effectiveness of educational workshops in improving the knowledge and attitude of attendees in laboratory animal science: A pre- and post-intervention design in Iran.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-24 DOI: 10.1177/00236772221109996

Siavash Ahmadi-Noorbakhsh, Jila Sadighi, Zahra Hatami, Ehsan Shamsi Gooshki

{"title":"The effectiveness of educational workshops in improving the knowledge and attitude of attendees in laboratory animal science: A pre- and post-intervention design in Iran.","authors":"Siavash Ahmadi-Noorbakhsh, Jila Sadighi, Zahra Hatami, Ehsan Shamsi Gooshki","doi":"10.1177/00236772221109996","DOIUrl":"https://doi.org/10.1177/00236772221109996","url":null,"abstract":"The knowledge and attitude of researchers can affect the validity of laboratory animal (LAN) research. However, studies show that not all researchers possess the required knowledge and appropriate attitude for performing valid research on animals. This may have several reasons, such as high heterogeneity in the educational backgrounds of researchers in animal trials. In this study, we hypothesized that properly designed intensive educational intervention could improve the knowledge and attitude of a mixed population of researchers, regardless of their heterogeneity. We delivered 10 country-wide two-day LAN workshops for academic members, postgraduate students and members of the ethics committees of the medical universities in Iran. Using pre-/post-intervention design, we found that the intervention highly significantly (p < 0.001) improved the knowledge score (from 2.96 ± 1.483 to 4.63 ± 1.548 mean ± SD; range: 0-8; n = 236) and attitude score (from 62.65 ± 5.160 to 65.57 ± 4.716; range: 14-70; N = 229). We also found that the educational intervention was significantly (p = 0.035) more effective to improve the knowledge of participants with more years of experience in LAN science. Unexpectedly, our younger participants (overall age range: 19-67 years old; mean ± SD: 37.96 ± 9.55) had lower initial attitude score. However, education was significantly (p = 0.002) more effective to improve the attitude score of younger participants. The knowledge and attitude improvements were not related to educational background, gender, history of prior attendance in LAN courses and amount of animal use. Attendees' performance in the final assessment showed that they require more education on the 'anesthesia/analgesia' topic. They also declared a high interest in learning more on the design of LAN studies.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772221109996"},"PeriodicalIF":1.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Case report: C57BL/6NTac and C57BL/6NCrl mice displaying neurological signs after deworming with ivermectin.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-24 DOI: 10.1177/00236772241286214

M Eriksson, S Nylén

{"title":"Case report: C57BL/6NTac and C57BL/6NCrl mice displaying neurological signs after deworming with ivermectin.","authors":"M Eriksson, S Nylén","doi":"10.1177/00236772241286214","DOIUrl":"https://doi.org/10.1177/00236772241286214","url":null,"abstract":"For over 40 years, ivermectin has served as an effective anti-parasitic drug used in human and veterinary medicine. In laboratory animal facilities it is used prophylactically or therapeutically to maintain the health status of the colony or experimentally in studies. Although ivermectin is generally safe to use, there are reports of neurotoxicity associated with ivermectin crossing the blood-brain barrier due to overdosing or blood-brain barrier dysfunction. In mice, P-glycoprotein maintains the blood-brain barrier and mice with a mutation in the P-glycoprotein encoding gene mdr1a are 50-100 times more sensitive to ivermectin. Signs of neurotoxicity include ataxia, bradypnea, recumbency, tremor, and death. We report neurotoxicity after ivermectin administration was used for the purpose of eradicating the murine-specific intestinal nematode Heligmosomoides polygyrus in C57BL/6NTac and C57BL/6NCrl mice. The mice were dewormed by subcutaneous administration of 10 or 20 mg/kg ivermectin to eradicate all stages of Heligmosomoides polygyrus. At 24-48h after deworming, 5% (n = 4) of the mice presented with tremor, ataxia, and/or head tilt. The affected mice were euthanised and gross pathological findings were found in one of the four mice (left-sided hydronephrosis). We assume that the observed neurological effects were due to defects in the blood-brain barrier, overdosing or individual sensitivity. This report provides a reason for caution when deworming laboratory mice subcutaneously with ivermectin at doses of 10 mg/kg or higher.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241286214"},"PeriodicalIF":1.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reusing zebrafish facility equipment: Planning, investment and what we have learned.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-23 DOI: 10.1177/00236772241274549

Olga M Fernández-Rodriguez, Emilio Cortés, Pablo Pelegrín, Laura Maldonado, Jesus Fernández, Jose Peñalver, Khrystyna Ostrovska, Maria T Fernández, Pablo Ramírez, Maria L Cayuela

{"title":"Reusing zebrafish facility equipment: Planning, investment and what we have learned.","authors":"Olga M Fernández-Rodriguez, Emilio Cortés, Pablo Pelegrín, Laura Maldonado, Jesus Fernández, Jose Peñalver, Khrystyna Ostrovska, Maria T Fernández, Pablo Ramírez, Maria L Cayuela","doi":"10.1177/00236772241274549","DOIUrl":"https://doi.org/10.1177/00236772241274549","url":null,"abstract":"The purpose of this study is to provide a detailed account of our successful experience in establishing a functional zebrafish holding facility by repurposing materials from a previous installation. On the eve of the start-up of our new animal facility we were notified that a research centre was putting part of its zebrafish holding facility (29 racks, accessories, water treatment unit) up for sale. Although the originally planned room was designed for six double racks, but encouraged by the increasing use of the zebrafish model, we decided to seize the opportunity, purchase the equipment and utilize it to create a larger configuration and an independent quarantine to protect the main facility. This decision inevitably led to extra expenses and situations, such as reinforcing the floor in the new location for the main facility, constructing an adjacent shelter for quarantine and adapting the racks. In spite of the additional costs, we consider the purchase and adaptation of this second-hand equipment for our facility to have been a success, and it has proven to be much more advantageous than acquiring brand new equipment. We trust that our experience will be of benefit to colleagues undertaking similar initiatives.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241274549"},"PeriodicalIF":1.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-immunization of diet-induced obese male mice with inactivated pathogens increases power in a liraglutide intervention study.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-18 DOI: 10.1177/00236772241279058

Caroline Falkenberg, Dorte B Sørensen, Camilla Hf Hansen, Martin F Toft, Axel K Hansen

{"title":"Pre-immunization of diet-induced obese male mice with inactivated pathogens increases power in a liraglutide intervention study.","authors":"Caroline Falkenberg, Dorte B Sørensen, Camilla Hf Hansen, Martin F Toft, Axel K Hansen","doi":"10.1177/00236772241279058","DOIUrl":"https://doi.org/10.1177/00236772241279058","url":null,"abstract":"Pre-immunization with inactivated antigens has been developed as an alternative to the use of 'dirty' mice, which in contrast to specific pathogen free (SPF) mice, harbour a range of pathogens. Within certain research areas, such mice are considered better models for humans than SPF mice, as they have an immune system that better mirrors human immunity. We inactivated murine adenovirus type 1 (FL), minute virus of mice, mouse hepatitis virus (A59), respirovirus muris (Sendai), Theiler's encephalomyelitis virus (GD7) and Mycoplasma pulmonis by ultraviolet irradiation. We show that pre-immunization with these inactivated pathogens combined with adjuvant prior to the dietary induction of obesity in C57BL/6NTac mice substantially reduced the group sizes needed for showing an effect of the GLP-1 receptor analogue, liraglutide. Nesting, open field and novel object behaviours of the mice were unaffected. We conclude that pre-immunization with inactivated pathogens may be a simple tool to increase power in this type of intervention study on the DIO mouse model.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241279058"},"PeriodicalIF":1.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comment on Frommlet and Heinze (2021): Correcting the error degrees of freedom.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-18 DOI: 10.1177/00236772241248718

Reid D Landes

引用次数: 0

Response to Landes: Comment on Frommlet and Heinze (2021): correcting the error degrees of freedom. 对 Landes 的回应：对 Frommlet 和 Heinze (2021) 的评论：修正误差自由度。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-18 DOI: 10.1177/00236772241276822

Georg Heinze, Florian Frommlet

引用次数: 0

Pitfalls in the analysis conditions of canine brain perfusion computed tomography.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-18 DOI: 10.1177/00236772241280013

Kazuya Kushida, Kodai Tashiro, Masaaki Katayama, Ryuji Fukushima, Miori Kishimoto

{"title":"Pitfalls in the analysis conditions of canine brain perfusion computed tomography.","authors":"Kazuya Kushida, Kodai Tashiro, Masaaki Katayama, Ryuji Fukushima, Miori Kishimoto","doi":"10.1177/00236772241280013","DOIUrl":"https://doi.org/10.1177/00236772241280013","url":null,"abstract":"This study aimed to investigate the impact of selected analysis conditions on blood flow values and color maps in canine brain perfusion computed tomography (PCT) and to propose optimal analysis conditions. Dynamic computed tomography imaging was performed on six beagle dogs. Color maps were generated using a combination of analysis algorithms (box-modulation transfer function (Box-MTF) and singular value deconvolution plus (SVD+) methods), slice thicknesses (4.0 and 8.0 mm), analysis matrix sizes (512 × 512, 256 × 256, and 128 × 128), and noise reduction levels (strong and weak). Cerebral blood flow (CBF) and cerebral blood volume (CBV) were calculated for gray matter, white matter, basal ganglia, hippocampus, thalamus, and cerebellum in each map. CBF and CBV values obtained using SVD+ were significantly higher than those obtained using Box-MTF. Noise reduction was more effective with larger matrix sizes; however, excessive noise reduction led to the blurring of anatomical structures in the color map. Across all analysis algorithms, anatomical structures were challenging to visualize at 8.0 mm. For canine brain PCT, it is essential to choose a straightforward algorithm that remains unaffected by circulatory velocity or intracranial bone structure. Given the brain's size, the slice thickness should be minimal, noise reduction level should be suitable for the targeted area, and matrix size should be maximized.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241280013"},"PeriodicalIF":1.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preceding sevoflurane inhalation in rats reduces the stress of intraperitoneal injection both in inexperienced experimenters and subject rats. 在大鼠吸入七氟醚之前，可减少没有经验的实验者和受试大鼠腹腔注射时的应激反应。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-18 DOI: 10.1177/00236772241279680

Hidetoshi Ishibashi, Hideyuki Motohashi

引用次数: 0

Brakes and leverages for reducing the environmental impact of laboratory animal facilities: A case study at the Institut Pasteur.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-14 DOI: 10.1177/00236772241280008

Marion Berard, Hélène da Conceiçao, Sébastien Bedu, Jean-Philippe Mocho

{"title":"Brakes and leverages for reducing the environmental impact of laboratory animal facilities: A case study at the Institut Pasteur.","authors":"Marion Berard, Hélène da Conceiçao, Sébastien Bedu, Jean-Philippe Mocho","doi":"10.1177/00236772241280008","DOIUrl":"https://doi.org/10.1177/00236772241280008","url":null,"abstract":"The Institut Pasteur has set the ambition to encourage all staff to get involved in sustainable development across all departments on campus. The animal facility staff joined the efforts of the sustainable development department to analyse current and future processes and identify potential solutions and related brakes and leverages for the reduction of the animal facilities' environmental impact. The first step was to collect the managers' experience on the local initiatives. Then, the managers attended a workshop to share information on networks and initiatives relevant to the topic and community, and to discuss the use of consumables, including personal protective equipment, single use plastics, bedding, feed, water, chemicals, and waste management. On the topics of interest, local initiatives were specifically detailed to assess their eventual implementation in all the institute's animal facilities, and tasks were set to pursue the managers' efforts in the longer term. A scenario was used to teach how to compare the carbon footprints of washing and disinfection equipment and process and to decide on the design of an animal facility. Finally, a summary is drawn of the brakes and leverages for the reduction of the environmental impact of the institute's animal facilities.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241280008"},"PeriodicalIF":1.3,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mouse strain-specific habituation to oral metamizole administration. 小鼠品系对口服甲咪唑的特异性习惯。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-12 DOI: 10.1177/00236772241274058

Tim Schreiber, Emily Leitner, Jakob Brandstetter, Anna Richter, Sandra Lange, Dietmar Zechner, Christian Junghanss, Brigitte Vollmar, Simone Kumstel

{"title":"Mouse strain-specific habituation to oral metamizole administration.","authors":"Tim Schreiber, Emily Leitner, Jakob Brandstetter, Anna Richter, Sandra Lange, Dietmar Zechner, Christian Junghanss, Brigitte Vollmar, Simone Kumstel","doi":"10.1177/00236772241274058","DOIUrl":"https://doi.org/10.1177/00236772241274058","url":null,"abstract":"When pain might occur during an animal experiment, sufficient analgesia is necessary. Metamizole is the third most used postoperative pain medication in animal research. The analgesic effect of metamizole is supposed to last 6-8 h in rodents. Therefore, the supplementation of drinking water with metamizole should be the preferred method to ensure permanent pain relief without unnecessary stressors. The present exploratory study compared the voluntary intake of metamizole-supplemented drinking water (3 mg/ml) between healthy mice of three different mouse strains. After the addition of metamizole to the drinking water, a marginal reduction in body weight was observed in C57BL/6J and BALB/c mice. However, NSG mice displayed a significantly higher body weight loss and reduction of drinking behavior compared with the C57BL/6J and BALB/c strains. The acceptance of metamizole in NSG mice did not increase with a different metamizole formulation. Thus, the mice of the inbred strains C57BL/6J and BALB/c seemed to be able to adapt to the taste of metamizole, while NSG mice were not able to accustom to analgesia within 1 week. Strain-specific habituation should be considered in future animal studies when analgesia is applied via drinking water.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241274058"},"PeriodicalIF":1.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0