Laboratory Animals最新文献_第2页

Inimitable leadership in animal care and program oversight. 在动物护理和项目监督方面具有无可比拟的领导能力。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-08-12 DOI: 10.1177/00236772261471002

F Claire Hankenson, Steven M Niemi

{"title":"Inimitable leadership in animal care and program oversight.","authors":"F Claire Hankenson, Steven M Niemi","doi":"10.1177/00236772261471002","DOIUrl":"https://doi.org/10.1177/00236772261471002","url":null,"abstract":"This reflective perspective on leadership in research animal care programs and the management of their operations aims to provide guiding principles, transferable across regulatory and institutional systems, for navigating current influences and future challenges. Inimitable leaders are those who embrace management with elevated professional standards, innovative insights, and sensitivity to the needs of multiple stakeholders. While a foundation in veterinary medicine and animal science is the most common background among directors of animal research programs, particularly those employed in the United States, managers and leaders everywhere need to be prepared for a future that will rely on clear communication, operational efficiency, skilled negotiations, overt investments in a Culture of Care and the 3Rs, and self-education for continuous improvement. As the future landscape of traditional animal models and translational research shifts irreversibly toward non-animal and novel alternative methodologies, so too should the inimitable leaders of tomorrow evolve to navigate the nuanced balance among animal welfare, biomedical discovery, and public scrutiny surrounding science for the benefit of humans and animals alike.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261471002"},"PeriodicalIF":1.3,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148712941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From exposure to evidence: The present and future of rodent surgical training in laboratory animal science. 从暴露到证据：实验动物科学中啮齿动物外科训练的现在和未来。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-08-09 DOI: 10.1177/00236772261470979

Diego Celdran-Bonafonte, Petra Seebeck

{"title":"From exposure to evidence: The present and future of rodent surgical training in laboratory animal science.","authors":"Diego Celdran-Bonafonte, Petra Seebeck","doi":"10.1177/00236772261470979","DOIUrl":"https://doi.org/10.1177/00236772261470979","url":null,"abstract":"Surgical training remains one of the most consequential yet historically underexamined components of laboratory animal science (LAS). While advances in anesthesia, analgesia, asepsis, and oversight have improved welfare and scientific rigor, training has often relied on decentralized apprenticeship models with variable structure and assessment, sometimes leaving foundational surgical standards such as aseptic workflow, sterile gowning, and gloving insufficiently formalized. As regulatory expectations, societal scrutiny, and research complexity have increased, the limitations of exposure-based training have become more apparent. This perspective examines the evolution of rodent surgical training in LAS, highlighting both the strengths of the modern training ecosystem and persistent gaps in standardization, access, and competency assessment. Emerging simulation technologies, including 3D-printed and hybrid models, offer scalable opportunities to reduce training-related animal use, shorten learning curves, and introduce objective performance benchmarks before live procedures. Evidence from surgical education and experimental microsurgery supports a hybrid, competency-based approach in which simulation prepares trainees for supervised live animal work rather than replacing it. Looking ahead, coordinated validation frameworks, shared assessment tools, and institutional commitment will be essential to transition toward a structured, data-driven, and ethically aligned post-apprenticeship model of laboratory animal surgical training.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261470979"},"PeriodicalIF":1.3,"publicationDate":"2026-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decision trees for managing genetically burdened laboratory animal lines under Directive 2010/63/EU. 根据指令2010/63/EU管理基因负担实验动物品系的决策树。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-07-30 DOI: 10.1177/00236772261459548

Myhailo Reshetnykov, Jan Baumgart

{"title":"Decision trees for managing genetically burdened laboratory animal lines under Directive 2010/63/EU.","authors":"Myhailo Reshetnykov, Jan Baumgart","doi":"10.1177/00236772261459548","DOIUrl":"https://doi.org/10.1177/00236772261459548","url":null,"abstract":"The breeding and maintenance of genetically modified laboratory animal lines with a harmful phenotype (hereafter referred to as \"genetically burdened lines\")-that is, lines in which pain, suffering, distress, or lasting harm may arise as an inherent consequence of the genotype-pose specific ethical and regulatory challenges for animal facilities and researchers. Within the European Union, these challenges are framed by Directive 2010/63/EU, which subjects genetically modified animals with harmful phenotypes and related breeding activities to project authorization and prospective severity assessment. Institutions responsible for such lines face recurrent practical questions: When does breeding constitute a regulated \"procedure\" requiring prior authorization? How should killing, tissue collection, and reuse be documented in local records and annual reports? In accordance with EU terminology, \"continued use\" refers to a single use consisting of multiple steps, whereas \"reuse\" describes the use of an animal in a new procedure after a previous one has been completed. And how should documentation differ between burdened and non-burdened phenotypes arising within the same colony? Here, we present decision trees that provide a structured and reproducible framework to support consistent decision-making for genetically burdened lines. The trees are illustrated using the German implementation of Directive 2010/63/EU (the German Animal Welfare Act, GAWA, and the German Laboratory Animal Welfare Ordinance, GLAWO) as a working example; however, the underlying decision logic is jurisdiction-neutral and could be adapted to other European and international regulatory systems by mapping national terminology to equivalent categories.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261459548"},"PeriodicalIF":1.3,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species-specific housing and routine procedures for spiny mice (Acomys) maintained in large individually ventilated cages. 在单独通风的大笼子中饲养的刺鼠（Acomys）的特定物种的住房和常规程序。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-07-25 DOI: 10.1177/00236772261458186

Antonio Tomasso, Aida Rodrigo Albors, Sofia Ferreira-Gonzalez, Ann Clemens, Vanessa A Disela, Enric Llorens Bobadilla, Sebastian A Lewandowski, Gustavo Tiscornia, Sofia Lamas, Monica M Sousa, Otto J M Mäkelä, Anna Meller, Satu Kuure, Tim Koopmans, Kerstin Bartscherer

{"title":"Species-specific housing and routine procedures for spiny mice (Acomys) maintained in large individually ventilated cages.","authors":"Antonio Tomasso, Aida Rodrigo Albors, Sofia Ferreira-Gonzalez, Ann Clemens, Vanessa A Disela, Enric Llorens Bobadilla, Sebastian A Lewandowski, Gustavo Tiscornia, Sofia Lamas, Monica M Sousa, Otto J M Mäkelä, Anna Meller, Satu Kuure, Tim Koopmans, Kerstin Bartscherer","doi":"10.1177/00236772261458186","DOIUrl":"https://doi.org/10.1177/00236772261458186","url":null,"abstract":"Spiny mice (Acomys spp.) are increasingly used in biomedical research owing to their unique physiological properties and ability to regenerate tissues, yet standardized laboratory housing conditions are largely based on mouse or rat models and may not adequately meet their species-specific needs. Here, we describe a practical protocol for the housing of spiny mice in large individually ventilated cages, including cage setup, diet, environmental enrichment, breeding, and health monitoring. We also provide recommendations for standard procedures used in animal research, such as identification methods, anesthesia, analgesia, and euthanasia. Last, we expand on the best laboratory practices in the context of common surgical procedures used in the field of regenerative biology, such as dorsal skin wounds and ear punch injury. Our recommendations are based on a decade of experience with routine colony management and behavioral observations at different European research sites and are intended as a practical reference for laboratories starting up Acomys colonies and as guidelines for regulatory authorities and ethics committees involved in approval processes for Acomys facilities and research.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261458186"},"PeriodicalIF":1.3,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148584705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to Advancing the 3Rs? Researchers' perspectives on institutional facilitation in Switzerland - Part 1: originative facilitation. 推进3r ？瑞士制度便利研究视角——第一部分：创新便利。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-07-23 DOI: 10.1177/00236772261464493

引用次数: 0

FELASA's accreditation for high-quality training in laboratory animal sciences. FELASA对实验动物科学高质量培训的认可。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-07-19 DOI: 10.1177/00236772261462981

Chris Van Ginneken, Magda João Castelhano-Carlos, Julia Steidle, Nicole Linklater, Nathalie Bourges Abella, Teresa De La Cueva Bueno, Bogdan Sevastre, Emanuella Rossi, Mats Sjöquist, Andreas Teubner

{"title":"FELASA's accreditation for high-quality training in laboratory animal sciences.","authors":"Chris Van Ginneken, Magda João Castelhano-Carlos, Julia Steidle, Nicole Linklater, Nathalie Bourges Abella, Teresa De La Cueva Bueno, Bogdan Sevastre, Emanuella Rossi, Mats Sjöquist, Andreas Teubner","doi":"10.1177/00236772261462981","DOIUrl":"10.1177/00236772261462981","url":null,"abstract":"The Federation of European Laboratory Animal Science Associations (FELASA) has played a pivotal role in ensuring high-quality education and training in laboratory animal sciences via accrediting courses. FELASA has revised its accreditation framework to reflect the evolving needs of the laboratory animal science community. This paper outlines the criteria for accrediting courses that train individuals for specific functions (A-D) (Article 23), roles (Articles 24(1)a-c; 25; 34; 38), Specialists in Laboratory Animal Sciences, and a selection of stand-alone modules. The accreditation process involves two steps: a self-assessment of the course and an on-site audit. Special attention is paid to constructive alignment, a tiered approach to skill acquisition, prioritising non-animal alternatives, ethical use of live animals, and structured assessments. FELASA's accreditation ensures that laboratory animal science education remains ethically responsible, scientifically robust and pedagogically sound.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261462981"},"PeriodicalIF":1.3,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term eradication of the chytrid fungus Batrachochytrium dendrobatidis from a large Xenopus laevis husbandry. 长期根除大型非洲爪蟾养殖中的壶菌水蛭壶菌。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-07-16 DOI: 10.1177/00236772261462356

Ulrike Teichmann, Sarah Kimmina

{"title":"Long-term eradication of the chytrid fungus Batrachochytrium dendrobatidis from a large Xenopus laevis husbandry.","authors":"Ulrike Teichmann, Sarah Kimmina","doi":"10.1177/00236772261462356","DOIUrl":"https://doi.org/10.1177/00236772261462356","url":null,"abstract":"The chytrid fungus Batrachochytrium dendrobatidis (BD) poses a serious threat to amphibian species worldwide. BD infects keratinocytes of the skin and interferes with their physiological barrier function. The African clawed frog Xenopus laevis is a species with low susceptibility to BD. Nevertheless, any infection presents a challenge to the immune system and, when combined with other stressors, carries the risk of overwhelming the body's defense. Infected animals may show greater variability in their scientific output than uninfected animals. This, in turn, means that more animals have to be used, which is not in line with the 3Rs principle. Moreover, releasing the fungus into the wastewater system should clearly be avoided to prevent infection of wild amphibians. Following confirmation of BD infection in the animals by Polymerase Chain Reaction (PCR), we decided to eradicate the fungus due to the reasons outlined above. This project involved not only the treatment of our frogs but also the timely coordinated disinfection of our entire permanently installed aquarium system. Itraconazole was applied as an immersion bath for the frogs and heat was used to disinfect the aquarium system. In this report, we outline our complex approach to a logistically demanding, labor-intensive project involving a large team of staff. We emphasize the need for straightforward planning, good communication and reporting on unexpected aspects and findings.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261462356"},"PeriodicalIF":1.3,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148456244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reporting and transparency in laboratory animal science: Past achievements, present concerns and future directions. 实验动物科学的报告和透明度：过去的成就，目前的关注和未来的方向。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-07-14 DOI: 10.1177/00236772261462343

Penny S Reynolds, Esther J Pearl

{"title":"Reporting and transparency in laboratory animal science: Past achievements, present concerns and future directions.","authors":"Penny S Reynolds, Esther J Pearl","doi":"10.1177/00236772261462343","DOIUrl":"https://doi.org/10.1177/00236772261462343","url":null,"abstract":"Transparent reporting of animal research is both a scientific and ethical imperative and is linked to both research quality and the principles of Replacement, Reduction, and Refinement (3Rs). Persistent concerns about poor reproducibility, limited translation and research waste have driven reforms in reporting standards and data-sharing requirements. However, despite considerable progress over the past 60 years, substantial gaps remain. Many published studies still lack essential methodological detail, such as animal characteristics, welfare measures, bias minimization methods (such as randomization and blinding), and sample size justification. Non-reporting of negative or null results further distorts the evidence base. A major barrier to change has been inadequate training of researchers in best practices for experimental design and statistical analysis. We describe how standardized reporting frameworks can promote research quality and highlight tools available to assist the researcher in implementing best practices in study design, reporting, and data accessibility. Over the next decade, emerging applications such as automated compliance tools and AI-assisted screening will offer scalable approaches to adoption of reporting standards. Preclinical science itself will be profoundly altered by advances in alternative models, open data, and linked machine-readable research outputs that will shift the research culture from isolated studies to an integrated cumulative evidence ecosystem. However, an expanded evidence base will be of value only if methodological quality is prioritized over novelty. Embedding best practices at study inception coupled with transparency will enhance reproducibility, reduce waste, and strengthen the reliability and translational value of animal research.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261462343"},"PeriodicalIF":1.3,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148447797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete Freund's adjuvant in laboratory animals: Reported welfare-relevant outcomes and alternatives-a scoping review. 实验动物的完全弗氏佐剂：报告的福利相关结果和替代方案-范围审查。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-07-11 DOI: 10.1177/00236772261459546

Valerie Hungerbühler, Agathe Verhulst, Cristian Berce, Otto Maissen, Beat Thomann, Luís Pedro Carmo

{"title":"Complete Freund's adjuvant in laboratory animals: Reported welfare-relevant outcomes and alternatives-a scoping review.","authors":"Valerie Hungerbühler, Agathe Verhulst, Cristian Berce, Otto Maissen, Beat Thomann, Luís Pedro Carmo","doi":"10.1177/00236772261459546","DOIUrl":"https://doi.org/10.1177/00236772261459546","url":null,"abstract":"Complete Freund's adjuvant (CFA) is used in laboratory animals to stimulate strong immune responses. However, it can cause persistent local inflammation and tissue damage, raising welfare concerns. This scoping review aimed to (i) assess how frequently welfare-relevant outcomes are reported in CFA immunisation and antibody-production studies in mice, rats and rabbits; (ii) describe reported welfare-relevant outcomes and the methods used to assess them; (iii) chart CFA regimen details across studies; and (iv) summarise reported alternatives to CFA, including welfare comparisons where available. We followed a protocol registered in Systematic Reviews for Animals and Food and reported in accordance with PRISMA-ScR. We searched PubMed and Embase from 2015 onwards. Screening was conducted in two phases (title/abstract; full text) by one reviewer, with uncertainties resolved by a second reviewer. Data were charted on animal characteristics, CFA regimen details, welfare monitoring, reported local/systemic outcomes, analgesia/humane endpoints and comparator adjuvants. Of 1608 records screened, 689 were assessed at full-text level and 43 met inclusion criteria by reporting a welfare-relevant outcome (including 'no adverse effects'): local outcomes were reported in 24/43 and systemic in 8/43; structured pain or behaviour monitoring occurred in 4/43 and formal scoring in 1/43 studies. Alternatives were evaluated in 32/43; in studies involving head-to-head comparisons with CFA (28/32), alternatives were more often better tolerated (22/28) than similar (6/28), with efficacy usually comparable or higher (31/32). Despite well-recognised welfare concerns with CFA, welfare-relevant outcomes are rarely reported. Standardised reporting of CFA regimens, welfare assessment methods and results is needed to support evidence-based refinement and comparison with alternatives.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261459546"},"PeriodicalIF":1.3,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can trained behavior serve as tool for welfare assessment? A pilot study in a cancer model with Wistar rats. 经过训练的行为可以作为福利评估的工具吗？用Wistar大鼠做癌症模型的初步研究。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-07-10 DOI: 10.1177/00236772261435375

Jennifer Mein, Marie-Luise H H Ranner-Hafferl, Dilyana B Mangarova, Jennifer L Heyl, Julia Brangsch, Avan Kader, Timo A Auer, Federico Collettini, Marcus G Doherr, Carola Fischer-Tenhagen

{"title":"Can trained behavior serve as tool for welfare assessment? A pilot study in a cancer model with Wistar rats.","authors":"Jennifer Mein, Marie-Luise H H Ranner-Hafferl, Dilyana B Mangarova, Jennifer L Heyl, Julia Brangsch, Avan Kader, Timo A Auer, Federico Collettini, Marcus G Doherr, Carola Fischer-Tenhagen","doi":"10.1177/00236772261435375","DOIUrl":"10.1177/00236772261435375","url":null,"abstract":"This pilot study investigates whether trained behavioral tasks may serve as early, non-invasive indicators of distress in high-severity disease models in laboratory rats. Therefore, six Wistar rats in a diethylnitrosamine-induced hepatocellular carcinoma model, were trained to perform two tasks: lockbox solving and scale climbing. They were assessed over 18 weeks by body weight, hair corticosterone (hCORT), and Grimace Scale. Changes in task performance time and hCORT levels were observed and revealed a three-phase pattern. Initially, poor task performance (mean lockbox solving time 22 s) and elevated hCORT (mean 10.8 pg/mg) indicated a process of adaptation to the experiment. This was followed by improved performance (mean lockbox solving time 2 s) and reduced hCORT (mean 7.9 pg/mg), suggesting acclimatization. Later, performance declined again (mean lockbox solving time 9 s), accompanied by rising hCORT (mean 8.3 pg/mg), likely reflecting tumor progression and repeated anesthesia exposure. Lockbox solving appeared more variable than scale climbing. Acute stressors, such as room changes and prolonged anesthesia, had an impact on lockbox solving and scale climbing. After the initial housing room change lockbox solving time increased from 20 s to 49 s while scale climbing time rose from 12 s to 26 s. Case observations confirmed that task performance deficits coincided with illness or larger tumor burden. These findings support the use of trained behavior as a sensitive tool to detect distress. This approach could improve welfare monitoring in long-term or high-severity animal studies.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261435375"},"PeriodicalIF":1.3,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0