Laboratory Animals最新文献_第2页

Darkness during behavioral tests induces exploratory activity and decreases anxiety-related behavior in male mice. 行为测试期间的黑暗诱导了雄性小鼠的探索活动并减少了与焦虑相关的行为。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-03-08 DOI: 10.1177/00236772261418962

Kseniya Avimova, Dmitry Sandakov

{"title":"Darkness during behavioral tests induces exploratory activity and decreases anxiety-related behavior in male mice.","authors":"Kseniya Avimova, Dmitry Sandakov","doi":"10.1177/00236772261418962","DOIUrl":"https://doi.org/10.1177/00236772261418962","url":null,"abstract":"Light is presumed to be highly aversive to laboratory rodents. It can change their physiological responses and activity. Much behavioral experimentation is conducted under dim light, though even dim light may alter the behavior of animals. Some research facilities use a reversed light regimen and provide darkness during routine and experimental procedures. However, there are no unified guidelines of light intensity during behavioral testing, and \"dim light\" is being understood very broadly. It can be suggested that testing in darkness can be ethologically relevant for mice, rats, and other rodents who are known for nocturnality in laboratory conditions. We hypothesized that laboratory mice would be more active when being tested in a dark room than in an illuminated one regardless of the circadian phase. To test our hypotheses we conducted an experiment to evaluate male outbred mice behavior in four standard tests widely used in preclinical studies: the open field test, the hole-board test, the tail suspension test, and the elevated plus maze test. Locomotion in the open field test increased in darkness. The hole-board test revealed significantly reduced exploratory activity when performed in a dark room. In the elevated plus maze, mice tested in darkness behave less anxiously than those tested in light. Illumination did not affect mobility of mice in the tail suspension test. We suppose that darkness reduces anxiety and, hence, induces locomotion and exploration in mice in tests that are provided to study these traits. The tail suspension test is aversive itself, so it may mask any environmental effects.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261418962"},"PeriodicalIF":1.3,"publicationDate":"2026-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sixty Years of Laboratory Animals. 实验动物的六十年。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2026-03-31 DOI: 10.1177/00236772261421513

Jan-Bas Prins

引用次数: 0

Advancing ethical standards in Göttingen Minipigs studies: The feasibility and reliability of Vascular Access Buttons™. 推进Göttingen迷你猪研究的伦理标准：血管访问按钮™的可行性和可靠性。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2025-11-24 DOI: 10.1177/00236772251380177

Mèlanie Reijnaers, Joelle van Dijk, Gabry Warmels, Adrian Zeltner, Judith Latour

{"title":"Advancing ethical standards in Göttingen Minipigs studies: The feasibility and reliability of Vascular Access Buttons™.","authors":"Mèlanie Reijnaers, Joelle van Dijk, Gabry Warmels, Adrian Zeltner, Judith Latour","doi":"10.1177/00236772251380177","DOIUrl":"10.1177/00236772251380177","url":null,"abstract":"The use of Vascular Access Buttons™ (VABs) is gaining traction in animal research as a less invasive alternative to traditional venipuncture methods and a superior option compared with existing alternatives, such as vascular access ports (VAPs). By enabling repeated blood collection and intravenous drug administration with reduced stress and discomfort for the animals, VABs contribute to improved data quality and enhanced animal welfare in animal studies. While increasingly used in rodents, their application in non-rodent species remains underexplored. This study evaluated the feasibility and reliability of the VAB system in two Göttingen Minipigs, comparing its performance with the conventional vena cava cranialis puncture. Pharmacokinetic assessments and clinical pathology analyses revealed consistent results across both techniques, demonstrating the VAB system's ability to generate reproducible, high-quality data. Additionally, its durability and ease of use highlight its potential as a practical and ethical alternative to both traditional venipunctures and VAPs in pharmacokinetic and long-term studies in minipigs. These findings support the integration of the VAB system in toxicological and pharmacokinetic research, particularly in studies requiring repeated blood collection or chronic intravenous dosing.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"85-95"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145587855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-specific histoarchitecture and functional correlation of the rat thyroid gland. 大鼠甲状腺性别特异性组织结构及功能相关性研究。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2025-12-08 DOI: 10.1177/00236772251390621

Ivan Milošević, Anja Nikolić, Emilija Nićković, Anita Radovanović, Bogomir Bolka Prokić, Jelena Jugović, Đurđica Marić, Aleksandra Buha Đorđević, Tijana Lužajić Božinovski

{"title":"Sex-specific histoarchitecture and functional correlation of the rat thyroid gland.","authors":"Ivan Milošević, Anja Nikolić, Emilija Nićković, Anita Radovanović, Bogomir Bolka Prokić, Jelena Jugović, Đurđica Marić, Aleksandra Buha Đorđević, Tijana Lužajić Božinovski","doi":"10.1177/00236772251390621","DOIUrl":"10.1177/00236772251390621","url":null,"abstract":"The thyroid gland's proper function is essential for controlling the metabolism, which varies to some extent between the sexes, thus maintaining the homeostasis. This research aimed to investigate differences in the histological structure and number of mast cells in the thyroid gland of male and female rats, and to establish whether there is a correlation between the levels of thyroid-stimulating hormone (TSH) and the activation index (Ia), which quantifies the functional activity of the gland based on histological parameters. Thyroid glands of 20 Wistar rats were analyzed morphometrically and stereologically to determine follicle size, distribution, and volume density of the epithelium, colloid, and connective tissue. Male thyroid glands had more small and medium follicles and a higher volume density of epithelium, while female thyroid glands had more large follicles and a higher volume density of connective tissue. The volume density of colloid was not significantly different between the sexes. The histological structure of the thyroid glands in both groups was in accordance with the measured TSH levels. The correlation between TSH serum levels and Ia were established in both sexes. Both the TSH serum levels and Ia were lower in females compared to males. A higher number of mast cells was noted in the connective tissue of female thyroid glands, compared to those of males, but this difference was not significant. The results of this study indicate that there are differences in the histological structure of male and female rat thyroid glands and that Ia can be used as a reliable complementary parameter for assessment of thyroid gland function.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"42-52"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical evaluation of intranasal midazolam and alfaxalone for induction of anaesthesia in rabbits. 咪达唑仑与阿法索龙鼻内诱导兔麻醉的临床评价。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2025-11-26 DOI: 10.1177/00236772251369171

Jennifer Hiekel, Peter Wm Kronen, Karina Klein

{"title":"Clinical evaluation of intranasal midazolam and alfaxalone for induction of anaesthesia in rabbits.","authors":"Jennifer Hiekel, Peter Wm Kronen, Karina Klein","doi":"10.1177/00236772251369171","DOIUrl":"10.1177/00236772251369171","url":null,"abstract":"The aim of this experimental, descriptive study was to evaluate feasibility, safety and side effects of alfaxalone and midazolam by intranasal instillation for anaesthesia induction in rabbits. We included 26, healthy, female New Zealand White Rabbits undergoing general anaesthesia in context of a study to test different coatings for stifle joint endoprosthesis. Midazolam (0.1 mg/kg) and alfaxalone 3 mg/kg (group 1) or 4 mg/kg (group 2) were mixed and administered intranasally. The number of sneezes, swallows and evasive attempts were recorded. Time to lateral recumbency, presence of salivation, nystagmus, induction and intubation qualities were scored. If intubation was not possible, a top-up of 1 mg/kg alfaxalone was administered intranasally. If still not sufficient, anaesthesia was induced by mask-insufflation of isoflurane. Data were analysed using IBM SPSS Statistics 30.0.0.0 and non-parametric data compared using either a Mann-Whitney test or a chi-square test. Overall, 10 animals assigned to group 1 and 16 animals to group 2 were included in the study. In 24/26 rabbits (92.3%) no significant complications were noted. One rabbit showed 20 s of apnoea after induction and one rabbit died during induction. Top-up dosages of alfaxalone were necessary in three cases and in two of these three, isoflurane administration was also required to complete anaesthetic induction. The median time to lateral recumbency was 32.5 s in group 1 and 15 s in group 2. By intranasal application of midazolam with alfaxalone at both dosages, the anaesthetic state was induced shortly after application.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"75-84"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145635000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FELASA recommendations for continuing professional development for professionals involved in animal research. FELASA对参与动物研究的专业人员持续专业发展的建议。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2025-12-09 DOI: 10.1177/00236772251361598

Magda João Castelhano-Carlos, Marcel Gyger, Chris Van Ginneken, Mats Sjöquist, Philippe Bugnon, Ana Isabel Santos

{"title":"FELASA recommendations for continuing professional development for professionals involved in animal research.","authors":"Magda João Castelhano-Carlos, Marcel Gyger, Chris Van Ginneken, Mats Sjöquist, Philippe Bugnon, Ana Isabel Santos","doi":"10.1177/00236772251361598","DOIUrl":"10.1177/00236772251361598","url":null,"abstract":"A continuing professional development (CPD) process should be implemented in all organisations involved in animal research to ensure professionals stay up to date with scientific advancements, best practices and regulations regarding animal welfare and laboratory animal science. These recommendations advocate for a lean and transparent CPD process to maintain the competence of professionals performing functions, roles and tasks outlined in Directive 2010/63/EU throughout a professional's career. CPD starts after initial training and once individual competence has been achieved. The first step involves creating a CPD portfolio, which the 'Person(s) Responsible for Training and Competence' reviews regularly to ensure it aligns with the professional's personal development plan (PDP). A structured portfolio is proposed for tracking and assessing CPD activities, enabling monitoring. We recommend this monitoring in cycles of up to 5 years.This document discusses the various types and formats of CPD activities, the minimum CPD requirements for professionals and the essential information that CPD certificates should include. This will be pivotal when developing a common framework for assessing CPD activities across institutions. To conclude, a harmonised CPD strategy that incorporates planning and record-keeping will facilitate mutual recognition and mobility of personnel. Moreover, promoting high-quality CPD will help motivate and reward staff while fostering a culture of care.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"31-41"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13031351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One size does not fit all: Guidelines for designing a score sheet for animal experiments - eight essential steps. 一种方式不适合所有：动物实验计分表设计指南——八个基本步骤。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2025-12-02 DOI: 10.1177/00236772251374409

Michaela Thallmair, María J Duque-Correa, Maike Heimann, Philippe Bugnon

引用次数: 0

Challenges and expectations on the use of automated home cage monitoring for advancing laboratory animal care and welfare. 使用自动化家庭笼监测促进实验动物护理和福利的挑战和期望。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2026-03-09 DOI: 10.1177/00236772251396319

Jordi L Tremoleda, Aurora Brønstad, Heidrun Potschka, Petra Seebeck, Oliver Stiedl, Vootele Voikar, Sara Wells

{"title":"Challenges and expectations on the use of automated home cage monitoring for advancing laboratory animal care and welfare.","authors":"Jordi L Tremoleda, Aurora Brønstad, Heidrun Potschka, Petra Seebeck, Oliver Stiedl, Vootele Voikar, Sara Wells","doi":"10.1177/00236772251396319","DOIUrl":"10.1177/00236772251396319","url":null,"abstract":"COST Action TEATIME unites experts to advance automated monitoring technologies for laboratory animals, with a focus on Home Cage Monitoring (HCM) systems. The use of HCM has great potential to revolutionise welfare monitoring by enabling continuous, non-invasive tracking of physiological and behavioural patterns in group-housed animals within their undisturbed housing environment. These systems capture spontaneous behaviours - such as feeding, grooming, social interactions and sleep cycles - across day and night phases, offering objective data for welfare and scientific assessments. This real-time monitoring might allow for early detection of distress, disease progression and subtle welfare changes, supporting timely interventions and refined humane endpoints. Unlike traditional clinical scoring, which relies on brief daily observations, HCM provides longitudinal, individualised insights and reduces observer bias. It might also facilitate better characterisation of positive affective states, contributing to more holistic welfare evaluations. Despite technological progress, challenges remain in data integration, sensitivity and standardisation across facilities. Effective implementation requires real-time alert capabilities, robust data management, and interdisciplinary collaboration among scientists, veterinarians and data experts. HCM systems should complement - not replace - human expertise, enriching welfare monitoring and scientific reproducibility. Their integration can improve husbandry, refine severity assessments, advancing both animal welfare and scientific replicability.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"25-30"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13031348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shelved cages impact affective behavior in Sprague Dawley rats - implications for animal welfare. 搁置的笼子影响斯普拉格·道利大鼠的情感行为——对动物福利的影响。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2025-11-24 DOI: 10.1177/00236772251384749

Logan J Bigelow, Emily K Pope, Clare Pl Lee, Veronica K Sohasky, Sarah K MacLeod, Paul B Bernard

{"title":"Shelved cages impact affective behavior in Sprague Dawley rats - implications for animal welfare.","authors":"Logan J Bigelow, Emily K Pope, Clare Pl Lee, Veronica K Sohasky, Sarah K MacLeod, Paul B Bernard","doi":"10.1177/00236772251384749","DOIUrl":"10.1177/00236772251384749","url":null,"abstract":"Laboratory animal welfare has received increasing attention in recent years as housing protocols move toward favoring environments that allow natural behaviors. Within this study, the effects of housing male and female Sprague Dawley rats in standard cages versus taller cages with an upper shelf were investigated. To determine differences in behavior and physiology based upon cage type, home-cage assessment of ultrasonic vocalizations and analysis of fecal corticosterone metabolites, as well as various behavioral tests, were performed. Rats in shelved cages produced significantly less 50 kHz calls, demonstrated better working memory in the spontaneous alternation task and had higher concentrations of fecal corticosterone metabolites. No differences were observed in the open field, elevated plus maze or light-dark box. While no significant treatment differences were found in the ultrasonic vocalization playback paradigm, results confirmed previous evidence of approach behavior upon 50 kHz call playback. The observed differences in behavior and physiology as a consequence of housing conditions inevitably have implications for experimental reproducibility as comparing studies across laboratories may be difficult if different housing parameters are utilized. The results of this study can also be used in guiding future animal welfare protocols given that certain cage modifications such as increased vertical space and/or the presence of a shelf might improve welfare. Investigation of additional parameters and strains of rodents will enhance our understanding of optimal laboratory animal housing conditions.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"53-62"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13031358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145587940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolated perfusion of pig kidney segments to study renal function: a translational model for human kidneys. 猪肾段离体灌注研究肾功能：人肾移植模型。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2025-10-14 DOI: 10.1177/00236772251369180

Thomas C Beham, Frank Schweda

{"title":"Isolated perfusion of pig kidney segments to study renal function: a translational model for human kidneys.","authors":"Thomas C Beham, Frank Schweda","doi":"10.1177/00236772251369180","DOIUrl":"10.1177/00236772251369180","url":null,"abstract":"Isolated kidney perfusion forms the bridge between in vitro and in vivo experiments and is mainly performed with rodent kidneys. It enables the controlled investigation of kidney function without systemic influences and is therefore currently, for instance, considered the gold standard for investigating the regulation of renin release, a central regulator of blood pressure. Renin secretion is controlled by several intrarenal signalling cascades and thus cannot be fully modelled in cell cultures. On the other hand, the in vivo investigation of renin release is confounded by systemic counter-regulatory systemic mechanisms. For ethical and logistical reasons, human kidney tissue has not been used for isolated organ perfusion yet. To reduce the use of rodents and to achieve the translation to larger species, including humans, we developed a method for isolated perfusion of slaughterhouse pig kidney segments. This method allows for future use with human kidney segments obtained from nephrectomies. Pig kidney segments were perfused at constant pressure and renin secretion was determined. The sympathomimetic agent isoproterenol, the Ca2+ chelator ethylene glycol tetraacetic acid, the NO-donor S-nitroso-N-acetylpenicillamine and the loop diuretic bumetanide stimulated renin release while angiotensin II suppressed it. These responses were similar to those observed in isolated perfused rodent kidneys, demonstrating that central regulatory mechanisms of renin release are conserved across species and in tissue segments. Moreover, the newly developed model of perfusion of kidney segments of large animals is suitable for the investigation of renin release from human kidneys and provides an ethical method for isolated kidney perfusion in accordance with animal welfare.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":"60 1","pages":"96-109"},"PeriodicalIF":1.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13031346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0