Laboratory Animals最新文献_第2页

Oestrous cycle synchronization protocols in rats using cloprostenol and progesterone injections. 使用氯前列醇和黄体酮注射的大鼠的发情周期同步方案。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-02-13 DOI: 10.1177/00236772241309754

Sophia Potapova, Viktor Stratilov, Oleg Vetrovoy, Diana Safarova, Ekaterina Tyulkova

引用次数: 0

Case report: C57BL/6NTac and C57BL/6NCrl mice displaying neurological signs after deworming with ivermectin. 病例报告：C57BL/6NTac和C57BL/6NCrl小鼠在伊维菌素驱虫后出现神经症状。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2024-12-24 DOI: 10.1177/00236772241286214

M Eriksson, S Nylén

{"title":"Case report: C57BL/6NTac and C57BL/6NCrl mice displaying neurological signs after deworming with ivermectin.","authors":"M Eriksson, S Nylén","doi":"10.1177/00236772241286214","DOIUrl":"10.1177/00236772241286214","url":null,"abstract":"For over 40 years, ivermectin has served as an effective anti-parasitic drug used in human and veterinary medicine. In laboratory animal facilities it is used prophylactically or therapeutically to maintain the health status of the colony or experimentally in studies. Although ivermectin is generally safe to use, there are reports of neurotoxicity associated with ivermectin crossing the blood-brain barrier due to overdosing or blood-brain barrier dysfunction. In mice, P-glycoprotein maintains the blood-brain barrier and mice with a mutation in the P-glycoprotein encoding gene mdr1a are 50-100 times more sensitive to ivermectin. Signs of neurotoxicity include ataxia, bradypnea, recumbency, tremor, and death. We report neurotoxicity after ivermectin administration was used for the purpose of eradicating the murine-specific intestinal nematode Heligmosomoides polygyrus in C57BL/6NTac and C57BL/6NCrl mice. The mice were dewormed by subcutaneous administration of 10 or 20 mg/kg ivermectin to eradicate all stages of Heligmosomoides polygyrus. At 24-48h after deworming, 5% (n = 4) of the mice presented with tremor, ataxia, and/or head tilt. The affected mice were euthanised and gross pathological findings were found in one of the four mice (left-sided hydronephrosis). We assume that the observed neurological effects were due to defects in the blood-brain barrier, overdosing or individual sensitivity. This report provides a reason for caution when deworming laboratory mice subcutaneously with ivermectin at doses of 10 mg/kg or higher.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"374-383"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breeding Planning for Laboratory Mice. 实验小鼠的繁殖计划。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-05-30 DOI: 10.1177/00236772251342983

Johannes Schenkel, Stefan Nagel-Riedasch, Branko Zevnik, Thorsten Buch

引用次数: 0

Inconsistent terminology in rodent anatomy hinders appropriate blood sampling in laboratory animal science: A review of blood sampling techniques on the head of mice. 啮齿动物解剖学中不一致的术语妨碍了实验动物科学中适当的血液采样：对小鼠头部血液采样技术的回顾。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-06-01 Epub Date: 2025-01-25 DOI: 10.1177/00236772241304504

Malan Štrbenc

引用次数: 0

Compassion fatigue in laboratory animal personnel: a bibliometric analysis of global trends. 实验动物人员的同情疲劳：全球趋势的文献计量学分析。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-05-30 DOI: 10.1177/00236772251332567

John K Chipangura

{"title":"Compassion fatigue in laboratory animal personnel: a bibliometric analysis of global trends.","authors":"John K Chipangura","doi":"10.1177/00236772251332567","DOIUrl":"https://doi.org/10.1177/00236772251332567","url":null,"abstract":"Laboratory animal personnel face enormous pressures such as workload, conflicts, deadlines, lack of psychological support, and ethical dilemmas. These pressures are often due to the need to balance scientific objectives, ethical responsibilities, and personal emotions, while trying to maintain legal and institutional animal housing and care standards. When these profession-related pressures are exacerbated by toxic social relationships and financial challenges, it may lead to chronic stress that will ultimately cause compassion fatigue. This review aimed to analyse publication trends on compassion fatigue among laboratory animal personnel by undertaking a bibliometric analysis of published articles to produce insights that could inform future research strategies. A literature search was carried out via the Scopus databases for articles published in English. The data collected from the search was transferred to the Bibliometrix R-package and assessed for publication trends, analysis of contributing countries, thematic evolution, and the co-occurrence of authors' keywords. Seventeen articles published between 2015 and 2024 were retrieved for analysis. Among these, 14 were survey studies and 3 were literature reviews. The number of articles published is on an upward trend, with all authors being affiliated with Global North institutions. The keyword analysis identified 53 words. The most common term used was 'compassion fatigue' (CF) and the analysis indicated that technicians and veterinarians are the primary target populations for CF research. The modest rise in the body of literature on CF and mental health issues among laboratory animal personnel is commendable and I encourage more work to be done to better understand this complex phenomenon.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251332567"},"PeriodicalIF":1.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Most preferred light color of female Syrian hamster during day and night. 雌性叙利亚仓鼠在白天和晚上最喜欢浅色。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-05-20 DOI: 10.1177/00236772251332402

Amir Farshad Shadman

引用次数: 0

Innovative cage-based technique for mouse urine collection. 创新的基于笼子的小鼠尿液收集技术。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-05-15 DOI: 10.1177/00236772251323627

Jianhua Huang, Yan Wang, Yan Gao, Angela Tornblom, Zerek Bianchi, Alexis Garcia, Katina Cahill, Gina Savastano

引用次数: 0

Insights and implications from a comparative analysis of in vitro fertilization success in Cavioidea and Muroidea rodents. 洞见和启示从体外受精成功的比较分析的鼠类和鼠。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-05-15 DOI: 10.1177/00236772251331683

Lhara Ricarliany Medeiros de Oliveira, Alexsandra Fernandes Pereira

{"title":"Insights and implications from a comparative analysis of in vitro fertilization success in Cavioidea and Muroidea rodents.","authors":"Lhara Ricarliany Medeiros de Oliveira, Alexsandra Fernandes Pereira","doi":"10.1177/00236772251331683","DOIUrl":"https://doi.org/10.1177/00236772251331683","url":null,"abstract":"In vitro fertilization (IVF) is an assisted reproduction technique widely used due to its capacity to generate more offspring within a brief timeframe while simultaneously providing fundamental physiological data through gamete interactions. However, its suboptimal development in most species is challenging when IVF is applied to rodents. Despite numerous studies focusing on the Cavioidea and Muroidea superfamilies, some species struggle to achieve satisfactory cleavage rates, whereas others obtain over 90% efficiency with live offspring. Efforts to enhance these rates include adopting methodologies commonly used in other mammals or developing entirely new protocols. Nonetheless, the distinct morphophysiological differences among these animals necessitate careful consideration to avoid overgeneralization during optimization. Therefore, we aimed to review what has been established for the two most researched rodent superfamilies, Cavioidea and Muroidea, regarding the steps of IVF, such as gamete manipulation and embryonic development, to understand better the protocol diversification in the rodent order and how it affects IVF efficiency. After an extensive analysis of data accumulated over the years, it becomes evident that the Muroidea superfamily is used more extensively in reproductive studies than the Cavioidea. Furthermore, IVF procedures achieve total efficiency in only one rodent species, the laboratory mouse (Mus musculus). Consequently, developing a successful IVF technique across rodent species requires substantial modifications to optimize species-specific early steps - a challenge complicated by difficulties in obtaining viable gametes during the initial stages and the limited research interest in species without significant commercial value, such as wild rodents.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251331683"},"PeriodicalIF":1.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accuracy of smartwatches for measuring heart rate and oxygen saturation in cynomolgus macaques compared to clinical standards. 智能手表测量食蟹猴心率和血氧饱和度的准确性与临床标准的比较。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-15 DOI: 10.1177/00236772251318910

Meghan Tiplady, Katya Douchant, Andrew N Winterborn

{"title":"Accuracy of smartwatches for measuring heart rate and oxygen saturation in cynomolgus macaques compared to clinical standards.","authors":"Meghan Tiplady, Katya Douchant, Andrew N Winterborn","doi":"10.1177/00236772251318910","DOIUrl":"https://doi.org/10.1177/00236772251318910","url":null,"abstract":"Continuous monitoring of physiological parameters in non-human primates (NHPs) necessitates a precise, non-invasive, and convenient method. This study aimed to validate the use of smartwatches with integrated pulse oximetry and heart rate (HR) monitoring capabilities for use in NHPs. Currently, the clinical standard for non-invasive continuous monitoring of peripheral oxygen saturation (SpO2) in NHPs has been the use of a transmittance pulse oximeter (TPO) affixed to a location of highly vascularized tissue. In a clinical setting, HR is monitored through electrocardiogram (ECG) or associated with SpO2 measurement from a TPO probe utilizing photoplethysmography technology. Challenges in obtaining precise readings with TPOs stem from technological limitations and probe placement restrictions. To address these limitations, simultaneous HR and SpO2 measurements were obtained from 15 cynomolgus macaques (Macaca fascicularis) using the Apple Watch 7 (AW 7), Apple Watch 9 (AW 9), and a clinical-grade TPO probe with integrated optical HR measurement technology (iM70, ELAN). Arterial blood gas (ABG) analysis was used as a reference method for SpO2. We found that a TPO device significantly underestimated SpO2 compared to the AW 7 and AW 9 when referenced against ABG values. Smartwatch-derived HR and SpO2 measurements demonstrated good agreement and minimal bias compared to the gold standard method. Overall, the AW 7 and AW 9 exhibited good agreement with clinical reference standards for HR and good agreement with the gold standard for SaO2 in sedated cynomolgus macaques.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251318910"},"PeriodicalIF":1.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new method to replace oral gavage for the study of Cryptosporidium infection in mice. 替代灌胃研究小鼠隐孢子虫感染的新方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-11 DOI: 10.1177/00236772251318406

Fernanda G Fumuso, Christine E Salomon, Roberta M O'Connor

{"title":"A new method to replace oral gavage for the study of Cryptosporidium infection in mice.","authors":"Fernanda G Fumuso, Christine E Salomon, Roberta M O'Connor","doi":"10.1177/00236772251318406","DOIUrl":"https://doi.org/10.1177/00236772251318406","url":null,"abstract":"Oral gavage is a widely used method to infect mice with Cryptosporidium parvum (CP), the most common animal model of infection. Cryptosporidium spp. are worldwide distributed, gastrointestinal parasites that mainly cause diarrhea in humans and neonatal ruminants. CP is transmitted via the fecal-oral route, usually through contaminated water. In rodent models of Cryptosporidium infection, oral gavage is used as the route of infection. This method, while effective, induces a generalized stress response and requires specific skills and experience. Our aim was to replace oral gavage by a refined method using a mixture palatable to mice, where oocysts could be mixed in. A peanut butter gelatin mix (PBG) containing CP oocysts was developed and used for voluntary oral infection of mice. We were able to confirm CP infections in young interferon-gamma knock out mice by detecting oocyst shedding in feces, demonstrating that the PBG method successfully produced infections similar to those obtain through oral gavage, and could be used for other pathogens or as a method to deliver experimental therapeutics or other substances.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251318406"},"PeriodicalIF":1.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0