Laboratory Animals最新文献_第2页

Non-invasive acquisition of vital data in anesthetized rats using laser and radar application 利用激光和雷达应用无创采集麻醉大鼠的生命数据

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-09-11 DOI: 10.1177/00236772241265541

Toshiaki Kawabe, Shota Kita, Isao Ohmura, Ryuji Michino, Hidenori Watanabe, Guanghao Sun, Seiya Inoue

{"title":"Non-invasive acquisition of vital data in anesthetized rats using laser and radar application","authors":"Toshiaki Kawabe, Shota Kita, Isao Ohmura, Ryuji Michino, Hidenori Watanabe, Guanghao Sun, Seiya Inoue","doi":"10.1177/00236772241265541","DOIUrl":"https://doi.org/10.1177/00236772241265541","url":null,"abstract":"The aim of this study was to verify the possibility of obtaining vital sign information using a laser and radar sensor in a manner that is non-invasive and painless for test animals. A dataset was obtained from respiratory movement of anaesthetized male F344 rats, signals of laser and radar sensors were recorded simultaneously with vital data acquired with an integrated multiple-channel intraoperative monitor. In addition, respiratory movements were also video recorded, and used as reference data of respiration rate (RR; ref-RR). Reference data for heart rate (HR; ref-HR) were obtained from the R wave of electrocardiogram data for each epoch. Signals recorded from the radar sensor (I- and Q-signals) were input to a computer, and HR (radar-HR) and RR (radar-RR) were estimated using the frequency analysis method. Among the six positions where respiratory movements were measured by the laser sensor, the number of peak counts matched the visual counts of respiratory movements in the video records. The respiratory movements were significantly the greatest over the most caudal rib in the dorsal ( p < 0.001). The average radar-RR and ref-RR values showed correspondence (ref-RR, 69 ± 6.2 breaths/min; radar-RR, 68 ± 5.7 breaths/min ( p = 0.04–1.00); equivalence ratio, 86%). The radar-HR data showed slight variability; however, there was 80% homology compared with the ref-HR values (ref-HR, 336 ± 19.6 beats/min; radar-HR, 348 ± 34.1 ( p = 0.10–0.95)). Although comparison of the data under noradrenaline administration failed to track drug-induced changes in some cases, the HR and RR data of anesthetized rats measured from the radar sensor system showed comparable accuracy to other conventional methods.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142178103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depicting variability and uncertainty using intervals and error bars. 使用区间和误差条描述可变性和不确定性。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-09-05 DOI: 10.1177/00236772241247105

Naomi Altman, Martin Krzywinski

引用次数: 0

Animal models for preventing seroma after surgery: a systematic review and meta-analysis. 预防术后血清肿的动物模型：系统回顾和荟萃分析。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-09-05 DOI: 10.1177/00236772241273010

Steven E M Poos, Bob P Hermans, Harry van Goor, Richard P G Ten Broek

{"title":"Animal models for preventing seroma after surgery: a systematic review and meta-analysis.","authors":"Steven E M Poos, Bob P Hermans, Harry van Goor, Richard P G Ten Broek","doi":"10.1177/00236772241273010","DOIUrl":"https://doi.org/10.1177/00236772241273010","url":null,"abstract":"Novel interventions for seroma prevention are urgently needed in clinical practice. Animal models are pivotal tools for testing these interventions; however, a significant translational gap persists between clinical and animal model outcomes. This systematic review aims to assess the methodological characteristics and quality of animal models utilized for seroma prevention. A meta-analysis was performed to estimate the expected seroma incidence rate for control groups and determine the effect size of typical interventions. We systematically retrieved all studies describing animal models in which seroma formation was induced. Methodological characteristics, risks of bias, and study quality were assessed. Seroma volume and -incidence data were used for the meta-analysis. In total, 55 studies were included, with 42 eligible for meta-analysis. Rats (69%) were the most frequently used species, with mastectomy (50%) being the predominant surgical procedure in these models. Despite significant risks of bias across all studies, an improving trend in reporting quality per decade was observed. The meta-analysis revealed an average seroma incidence of 90% in typical control groups. The average intervention halved the seroma incidence (RR = 0.49; CI 0.35, 0.70) and significantly reduced seroma volume (SMD = -3.31; CI -4.21, -2.41), although notable heterogeneity was present. In conclusion, animal models for seroma prevention exhibit methodological flaws and multiple risks of bias. Implementing sufficiently powered positive and negative control groups could improve the internal validity of these models. More research is needed for further development of animal seroma models.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The culture of care to enhance laboratory animal personnel well-being: a scoping review. 提高实验动物工作人员福利的关怀文化：范围审查。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-09-03 DOI: 10.1177/00236772241259089

Heidi L Morahan, Shari Cohen, Lisa Bero, Kieron B Rooney

{"title":"The culture of care to enhance laboratory animal personnel well-being: a scoping review.","authors":"Heidi L Morahan, Shari Cohen, Lisa Bero, Kieron B Rooney","doi":"10.1177/00236772241259089","DOIUrl":"https://doi.org/10.1177/00236772241259089","url":null,"abstract":"There has been intense focus on improving the quality of animal research in recent times. An emerging concept of a 'culture of care' has been proposed as another important pillar to enhance scientific quality, with staff well-being being a critical aspect. Professionals working with research animals can face moral and psychological burdens and are at risk of experiencing work-related stress. However, data on the global prevalence of stress in this population is limited. Equally, it is not clear how these stresses manifest, and what impact they might have on an individual's workplace performance and research quality. The purpose of this review was to identify work-related stress, its prevalence, and map evidence on strategies to mitigate stresses. We also set out to identify studies assessing the association between work-related stress and research quality. A systematic search was conducted across four databases, in addition to hand searching relevant references. We included peer-reviewed publications describing work-related stress, culture of care and laboratory animal professionals. A total of 49 publications were included for data mapping. Compassion fatigue was the most frequently described work-related stress, and its prevalence across Europe and North America is likely to be widespread. Multiple strategies to mitigate compassion fatigue and work-related stress were put forward, however, limited empirical evidence was available to assess success. Moreover, no studies reported empirical data linking work-related stress with research quality, despite several publications stating the case. Further population-specific research and measured assessments are urgently needed to deliver culture of care programmes to improve human well-being, animal welfare and research quality.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spontaneous type II cell-derived bronchioloalveolar adenoma in a young ICR mouse. 一只幼年 ICR 小鼠自发性 II 型细胞衍生支气管肺泡腺瘤。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-08-20 DOI: 10.1177/00236772241257726

Minkyoung Sung, Soo-Eun Sung, Joo-Hee Choi, Yujeong Lee, Kyung-Ku Kang, SunHee Park, Duyeol Kim, Sijoon Lee

引用次数: 0

Pharmacokinetics of single dose levobupivacaine after peri-incisional subcutaneous infiltration in anaesthetized domestic pigs. 麻醉家猪切口周围皮下注射单剂量左旋布比卡因的药代动力学。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-08-19 DOI: 10.1177/00236772241259618

Tilen Vake, Tomaž Snoj, Maja Čemažar, Urša Lampreht Tratar, Urban Stupan, Alenka Seliškar, Jan Plut, Tina Kosjek, Helena Plešnik, Marina Štukelj

{"title":"Pharmacokinetics of single dose levobupivacaine after peri-incisional subcutaneous infiltration in anaesthetized domestic pigs.","authors":"Tilen Vake, Tomaž Snoj, Maja Čemažar, Urša Lampreht Tratar, Urban Stupan, Alenka Seliškar, Jan Plut, Tina Kosjek, Helena Plešnik, Marina Štukelj","doi":"10.1177/00236772241259618","DOIUrl":"https://doi.org/10.1177/00236772241259618","url":null,"abstract":"Increasing use of pigs as models in translational research, and growing focus on animal welfare are leading to better use of effective analgesics and anaesthetics when painful procedures are performed. However, there is a gap in basic knowledge such as pharmacokinetics of different anaesthetics in these species. The main objective of our study was to determine the pharmacokinetics of levobupivacaine in domestic pigs. Twelve female grower pigs weighing 31.17 ± 4.6 kg were subjected to general anaesthesia and experimental surgery, at the end of which they received 1 mg/kg levobupivacaine via peri-incisional subcutaneous infiltration. Plasma samples were collected before administration of levobupivacaine and at 0.5, 1, 2, 4, 8, 12, 24 and 48 h thereafter. Concentrations of levobupivacaine were determined by liquid chromatography coupled with tandem mass spectrometry. Following single dose of levobupivacaine, all animals had measurable plasma concentrations 0.5 h after drug administration, with most peak concentrations observed at the 1-h time point. In all 12 animals, levobupivacaine was below the limit of quantification 48 h after drug administration. The mean maximum plasma concentration, area under the curve and half-life were determined to be 809.98 μg/l, 6552.46 μg/l h and 6.25 h, respectively. Plasma clearance, volume of distribution and weight-normalized volume of distribution were 4.41 l/h, 35.57 l and 1.23 l/kg, respectively. Peak plasma concentrations in our study were well below concentrations that were found to produce toxicity in pigs.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

About statistical significance, and the lack thereof. 关于统计意义，以及缺乏统计意义的问题。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-08-19 DOI: 10.1177/00236772241248509

Fulvio Magara, Benjamin Boury-Jamot

{"title":"About statistical significance, and the lack thereof.","authors":"Fulvio Magara, Benjamin Boury-Jamot","doi":"10.1177/00236772241248509","DOIUrl":"https://doi.org/10.1177/00236772241248509","url":null,"abstract":"Absence of statistical significance (i.e., p > 0.05) in the results of a frequentist test comparing two samples is often used as evidence of absence of difference, or absence of effect of a treatment, on the measured variable. Such conclusions are often wrong because absence of significance may merely result from a sample size that is too small to reveal an effect. To conclude that there is no meaningful effect of a treatment/condition, it is necessary to use an appropriate statistical approach. For frequentist statistics, a simple tool for this goal is the 'two one-sided t-test,' a form of equivalence test that relies on the a priori definition of a minimal difference considered to be relevant. In other words, the smallest effect size of interest should be established in advance. We present the principles of this test and give examples where it allows correct interpretation of the results of a classical t-test assuming absence of difference. Equivalence tests are also very useful in probing whether certain significant results are also biologically meaningful, because when comparing large samples it is possible to find significant results in both an equivalence test and in a two-sample t-test, assuming no difference as the null hypothesis.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ditching the norm: Using alternative distributions for biological data analysis. 抛弃常规：使用替代分布进行生物数据分析

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-08-19 DOI: 10.1177/00236772241246602

Stanley E Lazic

引用次数: 0

Variability in rat weight gain during development. 大鼠发育过程中体重增加的变化。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-08-19 DOI: 10.1177/00236772241246370

Pablo Vázquez-Borsetti

引用次数: 0

Treatment randomisation at animal or pen level? : Statistical analysis should follow the randomisation pattern! 在动物或栏的层面上进行治疗随机化？统计分析应遵循随机化模式！

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-08-19 DOI: 10.1177/00236772241247274

Luc Duchateau, Robrecht Dockx, Klara Goethals, Matthijs Vynck, Frédéric Vangroenweghe, Christian Burvenich

引用次数: 0