Laboratory Animals最新文献_第2页

An adaptable, user-friendly score sheet to monitor welfare in experimental fish.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-12 DOI: 10.1177/00236772241271013

Mathilde Flueck-Giraud, Heike Schmidt-Posthaus, Alessandra Bergadano, Irene Adrian-Kalchhauser

{"title":"An adaptable, user-friendly score sheet to monitor welfare in experimental fish.","authors":"Mathilde Flueck-Giraud, Heike Schmidt-Posthaus, Alessandra Bergadano, Irene Adrian-Kalchhauser","doi":"10.1177/00236772241271013","DOIUrl":"https://doi.org/10.1177/00236772241271013","url":null,"abstract":"Fish are increasingly used as experimental animals across research fields. Currently, around a quarter of all experimental animals used are fish. Less than 20% of these are standard model species. Welfare assessments for experimental fish are in their infancy compared with those for rodents. This can be attributed to the diversity of species used, the relative recency of fish as the go-to model for research, and challenges to assess welfare in non-vocal underwater species. The lack of guidelines and tools presents a challenge for researchers (particularly, for newcomers), for ethics committees and for implementing refinement measures. Here, we present an adaptable, user-friendly score sheet for fish based on MS Excel. The parameters are based on a literature review, have been validated by expert interviews and evaluated by a fish pathologist. The tool allows scoring of individual fish as well as groups, calculates summary scores and visualizes trends. We provide the underlying literature, give use examples and provide instructions on the adaptation and use of the score sheet. We hope that this tool will empower researchers to include welfare assessment in their routines, foster discussions on fish welfare parameters among scientists, facilitate interactions with ethics committees and, most importantly, enable the refinement of fish experiments.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241271013"},"PeriodicalIF":1.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Directive 2010/63/EU on research on wild-sourced animals: a review of publication trends in studies on captive wild mice (Apodemus sp.).

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-12 DOI: 10.1177/00236772241290404

Maílis Carrilho, Sophie von Merten, Maria da Luz Mathias, Joaquim T Tapisso

{"title":"Effects of Directive 2010/63/EU on research on wild-sourced animals: a review of publication trends in studies on captive wild mice (Apodemus sp.).","authors":"Maílis Carrilho, Sophie von Merten, Maria da Luz Mathias, Joaquim T Tapisso","doi":"10.1177/00236772241290404","DOIUrl":"https://doi.org/10.1177/00236772241290404","url":null,"abstract":"Most animals used in experimentation are small mammals. In the EU, Directive 2010/63/EU regulates the use of laboratory animals for experimental purposes. However, there are few guidelines for the use of wild-sourced animals, which cover permits, experimentation, transport, maintenance, and setting free after experiments. To evaluate the effect of the Directive on the study of wild-sourced animals, we conducted a systematised literature review focusing on three widespread rodents of the genus Apodemus: Apodemus agrarius, A. flavicollis and A. sylvaticus. We selected studies performed across the EU, published before (2000-August 2010), during (September 2010-2012) and after implementation of the Directive (2013-2022). From those, we collected data on three main topics: i) authorisation; ii) care and accommodation and iii) methods of killing. We found that after implementation of the Directive a higher proportion of published studies provided information about authorisation. In contrast, there was no significant difference over time in the information given about the care and accommodation of animals or methods of killing. As such, our analysis suggests that there is still room for improvement to achieve consistency across journals publishing research involving wild-sourced small mammals. Specifically, editors should require the provision of detailed information by authors regarding proper animal care (e.g. more detailed care and accommodation protocols). To harmonise the information requested by different editorial boards, we recommend the addition of specific guidelines in the Directive regarding wild animals, particularly on their proper accommodation, manipulation, enrichment and veterinary control.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241290404"},"PeriodicalIF":1.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To our esteemed colleague J-P.

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 DOI: 10.1177/00236772241299289

Jordi Tremoleda, Paulin Jirkof, Annemarie Lang, José M Sanchez-Morgado

引用次数: 0

Pharmacokinetics of single dose levobupivacaine after peri-incisional subcutaneous infiltration in anaesthetized domestic pigs. 麻醉家猪切口周围皮下注射单剂量左旋布比卡因的药代动力学。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI: 10.1177/00236772241259618

Tilen Vake, Tomaž Snoj, Maja Čemažar, Urša Lampreht Tratar, Urban Stupan, Alenka Seliškar, Jan Plut, Tina Kosjek, Helena Plešnik, Marina Štukelj

{"title":"Pharmacokinetics of single dose levobupivacaine after peri-incisional subcutaneous infiltration in anaesthetized domestic pigs.","authors":"Tilen Vake, Tomaž Snoj, Maja Čemažar, Urša Lampreht Tratar, Urban Stupan, Alenka Seliškar, Jan Plut, Tina Kosjek, Helena Plešnik, Marina Štukelj","doi":"10.1177/00236772241259618","DOIUrl":"10.1177/00236772241259618","url":null,"abstract":"Increasing use of pigs as models in translational research, and growing focus on animal welfare are leading to better use of effective analgesics and anaesthetics when painful procedures are performed. However, there is a gap in basic knowledge such as pharmacokinetics of different anaesthetics in these species. The main objective of our study was to determine the pharmacokinetics of levobupivacaine in domestic pigs. Twelve female grower pigs weighing 31.17 ± 4.6 kg were subjected to general anaesthesia and experimental surgery, at the end of which they received 1 mg/kg levobupivacaine via peri-incisional subcutaneous infiltration. Plasma samples were collected before administration of levobupivacaine and at 0.5, 1, 2, 4, 8, 12, 24 and 48 h thereafter. Concentrations of levobupivacaine were determined by liquid chromatography coupled with tandem mass spectrometry. Following single dose of levobupivacaine, all animals had measurable plasma concentrations 0.5 h after drug administration, with most peak concentrations observed at the 1-h time point. In all 12 animals, levobupivacaine was below the limit of quantification 48 h after drug administration. The mean maximum plasma concentration, area under the curve and half-life were determined to be 809.98 μg/l, 6552.46 μg/l h and 6.25 h, respectively. Plasma clearance, volume of distribution and weight-normalized volume of distribution were 4.41 l/h, 35.57 l and 1.23 l/kg, respectively. Peak plasma concentrations in our study were well below concentrations that were found to produce toxicity in pigs.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"602-611"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variability in rat weight gain during development. 大鼠发育过程中体重增加的变化。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI: 10.1177/00236772241246370

Pablo Vázquez-Borsetti

引用次数: 0

The culture of care to enhance laboratory animal personnel well-being: a scoping review. 提高实验动物工作人员福利的关怀文化：范围审查。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-09-03 DOI: 10.1177/00236772241259089

Heidi L Morahan, Shari Cohen, Lisa Bero, Kieron B Rooney

{"title":"The culture of care to enhance laboratory animal personnel well-being: a scoping review.","authors":"Heidi L Morahan, Shari Cohen, Lisa Bero, Kieron B Rooney","doi":"10.1177/00236772241259089","DOIUrl":"10.1177/00236772241259089","url":null,"abstract":"There has been intense focus on improving the quality of animal research in recent times. An emerging concept of a 'culture of care' has been proposed as another important pillar to enhance scientific quality, with staff well-being being a critical aspect. Professionals working with research animals can face moral and psychological burdens and are at risk of experiencing work-related stress. However, data on the global prevalence of stress in this population is limited. Equally, it is not clear how these stresses manifest, and what impact they might have on an individual's workplace performance and research quality. The purpose of this review was to identify work-related stress, its prevalence, and map evidence on strategies to mitigate stresses. We also set out to identify studies assessing the association between work-related stress and research quality. A systematic search was conducted across four databases, in addition to hand searching relevant references. We included peer-reviewed publications describing work-related stress, culture of care and laboratory animal professionals. A total of 49 publications were included for data mapping. Compassion fatigue was the most frequently described work-related stress, and its prevalence across Europe and North America is likely to be widespread. Multiple strategies to mitigate compassion fatigue and work-related stress were put forward, however, limited empirical evidence was available to assess success. Moreover, no studies reported empirical data linking work-related stress with research quality, despite several publications stating the case. Further population-specific research and measured assessments are urgently needed to deliver culture of care programmes to improve human well-being, animal welfare and research quality.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"515-529"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Animal models for preventing seroma after surgery: a systematic review and meta-analysis. 预防术后血清肿的动物模型：系统回顾和荟萃分析。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1177/00236772241273010

Steven E M Poos, Bob P Hermans, Harry van Goor, Richard P G Ten Broek

{"title":"Animal models for preventing seroma after surgery: a systematic review and meta-analysis.","authors":"Steven E M Poos, Bob P Hermans, Harry van Goor, Richard P G Ten Broek","doi":"10.1177/00236772241273010","DOIUrl":"10.1177/00236772241273010","url":null,"abstract":"Novel interventions for seroma prevention are urgently needed in clinical practice. Animal models are pivotal tools for testing these interventions; however, a significant translational gap persists between clinical and animal model outcomes. This systematic review aims to assess the methodological characteristics and quality of animal models utilized for seroma prevention. A meta-analysis was performed to estimate the expected seroma incidence rate for control groups and determine the effect size of typical interventions. We systematically retrieved all studies describing animal models in which seroma formation was induced. Methodological characteristics, risks of bias, and study quality were assessed. Seroma volume and -incidence data were used for the meta-analysis. In total, 55 studies were included, with 42 eligible for meta-analysis. Rats (69%) were the most frequently used species, with mastectomy (50%) being the predominant surgical procedure in these models. Despite significant risks of bias across all studies, an improving trend in reporting quality per decade was observed. The meta-analysis revealed an average seroma incidence of 90% in typical control groups. The average intervention halved the seroma incidence (RR = 0.49; CI 0.35, 0.70) and significantly reduced seroma volume (SMD = -3.31; CI -4.21, -2.41), although notable heterogeneity was present. In conclusion, animal models for seroma prevention exhibit methodological flaws and multiple risks of bias. Implementing sufficiently powered positive and negative control groups could improve the internal validity of these models. More research is needed for further development of animal seroma models.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"530-544"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guidance on the assessment of learning outcomes for those designing procedures and projects - Report of an ETPLAS Working Group. 程序和项目设计者学习成果评估指南--ETPLAS 工作组报告。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI: 10.1177/00236772241244527

Ismene A Dontas, Derek J Fry, David I Lewis, Kenneth Applebee, Martje Fentener van Vlissingen, Katerina Marinou, Johannes Schenkel

{"title":"Guidance on the assessment of learning outcomes for those designing procedures and projects - Report of an ETPLAS Working Group.","authors":"Ismene A Dontas, Derek J Fry, David I Lewis, Kenneth Applebee, Martje Fentener van Vlissingen, Katerina Marinou, Johannes Schenkel","doi":"10.1177/00236772241244527","DOIUrl":"10.1177/00236772241244527","url":null,"abstract":"This document provides assessment criteria for evaluation of each of the Learning Outcomes of the Modules specified (in addition to the Core Modules) for those designing procedures and projects in the Education and Training Framework guidance document by the European Commission and endorsed by the Member States Competent Authorities. This Working Group was tasked to produce these criteria by the Education & Training Platform for Laboratory Animal Science, which was funded by the European Commission to this aim. The assessment criteria address knowledge and skills (including critical thinking) expected to be acquired during education and training of persons preparing to design research procedures and projects using animals under the scope of Directive 2010/63/EU. Recognizing the diversity of expertise and experiences of those being educated and trained, we provide two levels of attainment, an ideal response and one that would be acceptable for each Learning Outcome. The balance between ideal and acceptable could be decided by the particular course providers and/or assessors, according to their local requirements. We envisage that the use of these assessment criteria by training providers and accrediting or approving bodies will help harmonize the education and training for those who will design procedures and projects using animals for scientific purposes. In Europe, this may also contribute to mutual recognition of training, and facilitate free movement of scientists.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"626-639"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fin clipping does not increase opercular beat rate in tricaine-treated zebrafish. 剪鳍不会增加三卡因处理斑马鱼的厣搏率。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI: 10.1177/00236772241252551

Martin D Burkhalter, Melanie Philipp

引用次数: 0

Establishment of reference intervals of haematology and biochemistry analytes in ICR mice of different ages. 确定不同年龄 ICR 小鼠血液学和生物化学分析指标的参考区间。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-09-30 DOI: 10.1177/00236772241260909

Suresh Patel, Satish Patel, Ashvin Kotadiya, Samir Patel, Bhavesh Shrimali, Tushar Patel, Harshida Trivedi, Vishal Patel, Jogeswar Mahapatra, Mukul Jain

{"title":"Establishment of reference intervals of haematology and biochemistry analytes in ICR mice of different ages.","authors":"Suresh Patel, Satish Patel, Ashvin Kotadiya, Samir Patel, Bhavesh Shrimali, Tushar Patel, Harshida Trivedi, Vishal Patel, Jogeswar Mahapatra, Mukul Jain","doi":"10.1177/00236772241260909","DOIUrl":"10.1177/00236772241260909","url":null,"abstract":"Outbred stocks of mice are widely used in pre-clinical research as these animals possess a diversified genetic background when compared with inbred strains of mice. It is crucial to assess particular alterations in the physiological and functional profiles of laboratory animals using haematological and biochemical indicators. These values can also differ between laboratories because they are influenced by many different factors. We aimed to provide normal values and reference intervals for selected haematology and biochemistry analytes of 570 ICR mice at three different ages: 6-8 weeks, 10-14 weeks and 6-9 months. Reference values were calculated by non-parametric methods. For comparisons between sexes, the independent-sample t-test and Mann-Whitney test were employed, and analysis of variance was used for age differences. The findings of the study revealed age-related declines in haemoglobin concentration, haematocrit, mean corpuscular volume and mean corpuscular haemoglobin concentrations. Mice aged 6-9 months had statistically higher platelet counts in their blood than mice of other ages. The white blood cell count had a significant age effect and progressively decreased with age. As mice get older, the percentage of neutrophils, monocytes and basophils increases, but the percentage of lymphocytes decreases. For the biochemical values, age-related significant differences in glucose, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and albumin concentrations were found. It was also found that creatinine concentrations were comparable across all age ranges. The values presented in the present work can be used as a reference to interpret clinical pathology data for other studies and to evaluate health status.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"565-578"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0