Laboratory Animals最新文献_第3页

Our 61st Annual Meeting: An exciting programme is shaping up! 我们的第 61 届年会：令人兴奋的活动即将开始！

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 DOI: 10.1177/00236772241279473

Jordi L Tremoleda

引用次数: 0

Preclinical pilot studies: Five common pitfalls and how to avoid them. 临床前试验研究：五个常见陷阱及如何避免。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-08-05 DOI: 10.1177/00236772241244519

Natasha A Karp, Alan Sharpe, Benjamin Phillips

引用次数: 0

Treatment randomisation at animal or pen level? : Statistical analysis should follow the randomisation pattern! 在动物或栏的层面上进行治疗随机化？统计分析应遵循随机化模式！

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-08-19 DOI: 10.1177/00236772241247274

Luc Duchateau, Robrecht Dockx, Klara Goethals, Matthijs Vynck, Frédéric Vangroenweghe, Christian Burvenich

引用次数: 0

Heterogeneity of animal experiments and how to deal with it. 动物实验的异质性及应对方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-09-24 DOI: 10.1177/00236772241260173

Bernhard Voelkl, Hanno Würbel

{"title":"Heterogeneity of animal experiments and how to deal with it.","authors":"Bernhard Voelkl, Hanno Würbel","doi":"10.1177/00236772241260173","DOIUrl":"10.1177/00236772241260173","url":null,"abstract":"Heterogeneity of study samples is ubiquitous in animal experiments. Here, we discuss the different options of how to deal with heterogeneity in the statistical analysis of a single experiment. Specifically, data from different sub-groups (e.g. sex, strain, age cohorts) may be analysed separately, heterogenization factors may be ignored and data pooled for analysis, or heterogenization factors may be included as additional variables in the statistical model. The cost of ignoring a heterogenization factor is an inflated estimate of the variance and a consequent loss of statistical power. Therefore, it is usually preferable to include the heterogenization factor in the statistical model, especially if the heterogenization factor has been introduced intentionally (e.g. using both sexes). If heterogenization factors are included, they can be treated either as fixed factors in an analysis of variance design or sometimes as random effects in mixed effects regression models. Finally, for an appropriate sample size estimation, it is necessary to decide whether to treat heterogenization factors as nuisance variables, or whether the experiment should be powered to be able to detect not only the main effect of the treatment but also interactions between heterogenization factors and the treatment variable.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vacancy for EDITOR position to join the EIC team. 编辑职位空缺，请加入 EIC 团队。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-09-20 DOI: 10.1177/00236772241281044

引用次数: 0

Causal mediation analysis: How to avoid fooling yourself that X causes Y. 因果中介分析：如何避免自欺欺人地认为 X 会导致 Y。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-08-11 DOI: 10.1177/00236772231217777

Stanley E Lazic

引用次数: 0

Incorporating sources of correlation between outcomes: An introduction to mixed models. 纳入结果之间的相关性来源：混合模型简介。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-09-20 DOI: 10.1177/00236772241259518

Limeng Liu, Ashley Petersen

{"title":"Incorporating sources of correlation between outcomes: An introduction to mixed models.","authors":"Limeng Liu, Ashley Petersen","doi":"10.1177/00236772241259518","DOIUrl":"10.1177/00236772241259518","url":null,"abstract":"Animal research often involves measuring the outcomes of interest multiple times on the same animal, whether over time or for different exposures. These repeated outcomes measured on the same animal are correlated due to animal-specific characteristics. While this repeated measures data can address more complex research questions than single-outcome data, the statistical analysis must take into account the study design resulting in correlated outcomes, which violate the independence assumption of standard statistical methods (e.g. a two-sample t-test, linear regression). When standard statistical methods are incorrectly used to analyze correlated outcome data, the statistical inference (i.e. confidence intervals and p-values) will be incorrect, with some settings leading to null findings too often and others producing statistically significant findings despite no support for this in the data. Instead, researchers can leverage approaches designed specifically for correlated outcomes. In this article, we discuss common study designs that lead to correlated outcome data, motivate the intuition about the impact of improperly analyzing correlated outcomes using methods for independent data, and introduce approaches that properly leverage correlated outcome data.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulation methodologies to determine statistical power in laboratory animal research studies. 确定实验动物研究统计能力的模拟方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-09-24 DOI: 10.1177/00236772241273002

Angela Jeffers, Kathryn Konrad, Gary Larson, Katherine Allen-Moyer, Helen Cunny, Keith Shockley

引用次数: 0

Establishment of reference intervals of haematology and biochemistry analytes in ICR mice of different ages. 确定不同年龄 ICR 小鼠血液学和生物化学分析指标的参考区间。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-09-30 DOI: 10.1177/00236772241260909

Suresh Patel, Satish Patel, Ashvin Kotadiya, Samir Patel, Bhavesh Shrimali, Tushar Patel, Harshida Trivedi, Vishal Patel, Jogeswar Mahapatra, Mukul Jain

{"title":"Establishment of reference intervals of haematology and biochemistry analytes in ICR mice of different ages.","authors":"Suresh Patel, Satish Patel, Ashvin Kotadiya, Samir Patel, Bhavesh Shrimali, Tushar Patel, Harshida Trivedi, Vishal Patel, Jogeswar Mahapatra, Mukul Jain","doi":"10.1177/00236772241260909","DOIUrl":"https://doi.org/10.1177/00236772241260909","url":null,"abstract":"Outbred stocks of mice are widely used in pre-clinical research as these animals possess a diversified genetic background when compared with inbred strains of mice. It is crucial to assess particular alterations in the physiological and functional profiles of laboratory animals using haematological and biochemical indicators. These values can also differ between laboratories because they are influenced by many different factors. We aimed to provide normal values and reference intervals for selected haematology and biochemistry analytes of 570 ICR mice at three different ages: 6-8 weeks, 10-14 weeks and 6-9 months. Reference values were calculated by non-parametric methods. For comparisons between sexes, the independent-sample t-test and Mann-Whitney test were employed, and analysis of variance was used for age differences. The findings of the study revealed age-related declines in haemoglobin concentration, haematocrit, mean corpuscular volume and mean corpuscular haemoglobin concentrations. Mice aged 6-9 months had statistically higher platelet counts in their blood than mice of other ages. The white blood cell count had a significant age effect and progressively decreased with age. As mice get older, the percentage of neutrophils, monocytes and basophils increases, but the percentage of lymphocytes decreases. For the biochemical values, age-related significant differences in glucose, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and albumin concentrations were found. It was also found that creatinine concentrations were comparable across all age ranges. The values presented in the present work can be used as a reference to interpret clinical pathology data for other studies and to evaluate health status.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jacketed telemetry in rats: a novel non-invasive method for cardiorespiratory phenotyping during treadmill exercise. 大鼠夹套遥测：跑步机运动中心肺表型的新型无创方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-09-30 DOI: 10.1177/00236772241259857

Stéphane Tanguy, Agathe Cambier, Leandro Fontana-Pires, Timothé Flenet, Charles Eynard, Julie Fontecave-Jalon, Pierre-Yves Gumery, François Boucher

{"title":"Jacketed telemetry in rats: a novel non-invasive method for cardiorespiratory phenotyping during treadmill exercise.","authors":"Stéphane Tanguy, Agathe Cambier, Leandro Fontana-Pires, Timothé Flenet, Charles Eynard, Julie Fontecave-Jalon, Pierre-Yves Gumery, François Boucher","doi":"10.1177/00236772241259857","DOIUrl":"https://doi.org/10.1177/00236772241259857","url":null,"abstract":"The development of alternative methods for monitoring cardiorespiratory function without restraint or surgical implantation is attracting growing interest for both ethical and scientific reasons. For this purpose, a new non-invasive jacketed telemetry tool consisting in a radio device maintained in a jacket worn by the animal was previously developed to improve cardiorespiratory monitoring. It allows simultaneous monitoring of cardiac activity by surface electrocardiagram, respiratory function by respiratory inductive plethysmography, and locomotor activity by accelerometry. However, this tool has only been validated under conditions of low/intermediate activity levels or in anesthetized animals. This study aimed to evaluate the feasibility of using this system in the challenging conditions of an exertion protocol. Male Wistar rats (n = 10, 8-9 weeks old) were subjected to an incremental treadmill exercise protocol including speed levels from 5 to 40 cm s-1 separated by 30-s breaks. Heart rate (HR) and minute ventilation (assessed by minute volume; MV) were continuously monitored. At the end of each running level and during the 30-s breaks, HR and MV showed a significant increase compared to resting values. They returned to the baseline within 60 min of post-exercise recovery. Overall, our results demonstrated (i) the ability of the animal to run while wearing the device and (ii) the ability of the device to reliably monitor cardiorespiratory adaptation to treadmill exercise despite significant mechanical disturbances. In conclusion, this study highlights the possibility of non-invasively monitoring cardiorespiratory functional variables that were previously unattainable under conditions of high activity in freely moving animals.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0