发布求助
文献互助 智能选刊 最新文献

Laboratory Animals最新文献

筛选
英文 中文
Fin clipping does not increase opercular beat rate in tricaine-treated zebrafish. 剪鳍不会增加三卡因处理斑马鱼的厣搏率。
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-19 DOI: 10.1177/00236772241252551
Martin D Burkhalter, Melanie Philipp
{"title":"Fin clipping does not increase opercular beat rate in tricaine-treated zebrafish.","authors":"Martin D Burkhalter, Melanie Philipp","doi":"10.1177/00236772241252551","DOIUrl":"https://doi.org/10.1177/00236772241252551","url":null,"abstract":"<p><p>To carry out research with genetically modified animals, their genotype has to be assessed. A standard protocol to obtain required tissue samples from zebrafish is finclipping. However, some studies reported considerable stress induced by this protocol. We therefore assessed ventilation as a read-out for stress in zebrafish that underwent finclipping during routine genotyping in our fish facility. Our analysis could not confirm a strong increase of ventilation as had been previously reported. Instead, handled zebrafish showed ventilation rates in the range of controls that remained in their holding tanks. Additionally, we detected a slight reduction of ventilation rates up to an hour after anaesthesia in zebrafish treated with tricaine only, suggesting a prolonged protecting effect by this anaesthetic.</p>","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of two bedding types on the breeding fecundity of C57BL/6J mice. 两种垫料对 C57BL/6J 小鼠繁殖力的影响比较
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-19 DOI: 10.1177/00236772241249783
Jessica L Pinckard, Lauren A Gibson, Wendy R Williams
{"title":"Comparison of two bedding types on the breeding fecundity of C57BL/6J mice.","authors":"Jessica L Pinckard, Lauren A Gibson, Wendy R Williams","doi":"10.1177/00236772241249783","DOIUrl":"https://doi.org/10.1177/00236772241249783","url":null,"abstract":"<p><p>One of the favored options for generating complex transgenic laboratory mice is through in-house breeding and management strategies. One consideration in the management of these colonies is how the animals' environment may affect reproductive success. Several aspects of the microenvironment can be controlled or manipulated, including cage type, bedding, enrichment, diet, and temperature and humidity. This study sought to evaluate reproductive outcomes for C57BL/6J mice that were randomly assigned to one of two different bedding types: paper based or corncob bedding. Our hypothesis was there would be no significant difference in reproductive outcomes between the two bedding types. A total of 10 males and 10 females were paired at 45 days of age. Animals were allowed to breed for 15 consecutive weeks. Cages were checked daily for the presence of pups and a pup count was performed at 7 days of age. Weaning occurred at 20 or 21 days of age, at which time a final pup count, pup weight, and sex were recorded. All litters born and pups weaned in the 15-week timeframe were used for data analysis. Statistical analysis compared cannibalization between the two groups and the results showed no statistical difference between groups (<i>p</i> > 0.05). Other parameters analyzed included average litter size, average weaning weight, and number of litters per group. All pups counted at Day 7 survived to weaning age in both groups. We concluded that both bedding types produced similar success regarding breeding fecundity in C57BL/6J mice.</p>","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Refining urine collection in mice: Development of an innovative urine collection device. 改进小鼠的尿液收集：开发创新型尿液收集装置
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-19 DOI: 10.1177/00236772231219828
Mélodie Douté, Céline Monzali, Antonino Nicoletti, Giuseppina Caligiuri, Marc Clement
{"title":"Refining urine collection in mice: Development of an innovative urine collection device.","authors":"Mélodie Douté, Céline Monzali, Antonino Nicoletti, Giuseppina Caligiuri, Marc Clement","doi":"10.1177/00236772231219828","DOIUrl":"https://doi.org/10.1177/00236772231219828","url":null,"abstract":"<p><p>Urine collection can be challenging in studies involving small rodents like mice, as the actual methods of collection are anxiogenic and constrain animal welfare while having high variability in the volume of urine collected. To improve the current methods and eventually reduce the impact on the well-being of mice, we developed an innovative 3D-printed urine collection device (UCD). This two-compartment UCD is shaped to fit in classical husbandry cages and allows urine collection by spontaneous urination from two mice housed in their own cage without cross-contamination while enabling potential social interactions. We used our UCD to study the evolution of urinary parameters related to renal functions in a model of antibody-mediated chronic kidney disease. Overall, we report here a time-saving and affordable method for urine collection providing a large amount of uncontaminated urine and which we believe may improve animal welfare in comparison with other methods.</p>","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Causal mediation analysis: How to avoid fooling yourself that X causes Y. 因果中介分析：如何避免自欺欺人地认为 X 会导致 Y。
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-11 DOI: 10.1177/00236772231217777
Stanley E Lazic
{"title":"Causal mediation analysis: How to avoid fooling yourself that <i>X</i> causes <i>Y</i>.","authors":"Stanley E Lazic","doi":"10.1177/00236772231217777","DOIUrl":"https://doi.org/10.1177/00236772231217777","url":null,"abstract":"<p><p>The purpose of many preclinical studies is to determine whether an experimental intervention affects an outcome through a particular mechanism, but the analytical methods and inferential logic typically used cannot answer this question, leading to erroneous conclusions about causal relationships, which can be highly reproducible. A causal mediation analysis can directly test whether a hypothesised mechanism is partly or completely responsible for a treatment's effect on an outcome. Such an analysis can be easily implemented with modern statistical software. We show how a mediation analysis can distinguish between three different causal relationships that are indistinguishable when using a standard analysis.</p>","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Facility design and management. Strategies for high level biocontainment. 设施设计和管理。高水平生物隔离战略。
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-09 DOI: 10.1177/00236772241247104
Mike Dennis, Michael Charlton, Samantha Elam, Sarah Wolfensohn
{"title":"Facility design and management. Strategies for high level biocontainment.","authors":"Mike Dennis, Michael Charlton, Samantha Elam, Sarah Wolfensohn","doi":"10.1177/00236772241247104","DOIUrl":"https://doi.org/10.1177/00236772241247104","url":null,"abstract":"<p><p>UK Health Security Agency is required to investigate the pathogenesis of emerging or re-emerging infections and to test novel interventions, such as vaccines and therapeutics against these and other diseases, such as tuberculosis and Ebola, that have a significant impact on human health world-wide. Research into the causative agents (mainly BSL 3 and 4) using a wide range of animal species as pre-clinical models brings a number of challenges in terms of effective biocontainment to address human safety whilst optimising delivery of scientific objectives and the welfare of the animals. Here we describe the strategies used for high containment of species that include mice, ferrets, hamsters, rabbits and macaques that have been infected with high consequence pathogens. To ensure relevance of these models we frequently challenge by the aerosol route and monitor the development of disease and protective or therapeutic efficacy by methodologies similar to those used in the clinic. We have devised methods of sampling that can inform on pathogenesis and immune function that include lung lavage and medical imaging such as computed tomography and positron emission tomography-computed tomography. Imaging assists our assessment of progression to disease whilst providing refinement in application of early humane endpoints. We have developed directional flow containment systems that provide quantifiable operator protection whilst allowing group housing and a wide range of enrichment strategies appropriate for each species. Furthermore, we have demonstrated our improvements in animal welfare through use of a software-based Animal Welfare Assessment Grid that was developed with help of NC3Rs funding and enables us to quantify the lifetime experience of animals.</p>","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preclinical pilot studies: Five common pitfalls and how to avoid them. 临床前试验研究：五个常见陷阱及如何避免。
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-05 DOI: 10.1177/00236772241244519
Natasha A Karp, Alan Sharpe, Benjamin Phillips
{"title":"Preclinical pilot studies: Five common pitfalls and how to avoid them.","authors":"Natasha A Karp, Alan Sharpe, Benjamin Phillips","doi":"10.1177/00236772241244519","DOIUrl":"https://doi.org/10.1177/00236772241244519","url":null,"abstract":"<p><p>Pilots are small-scale initial experiments that are intended to guide the design of future, larger studies, with a view to increasing their effectiveness. In this statistical primer we highlight five common mistakes that limit the utility of pilot studies and provide practical guidance to avoid such errors and increase their effectiveness. The common thread connecting these mistakes is insufficient planning and over-interpretation of the results. This approach compromises the ultimate goals of the research programme and the future experimental cascade. In support of our view that over-interpretation is an error, we present a simple simulation to demonstrate that pilots will generally generate an inaccurate estimate of the variability of the biological endpoint under study and that frequent under-estimation will lead to inconclusive and unethical subsequent experiments. We argue that well planned pilots are an important part of the research cascade and still need to be implemented to a high standard.</p>","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LAS Around the Globe - United States of America. 全球各地的 LAS - 美国。
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-01 Epub Date: 2024-06-11 DOI: 10.1177/00236772231225896
Jennie Lofgren, Kelly Metcalf-Pate
{"title":"LAS Around the Globe - United States of America.","authors":"Jennie Lofgren, Kelly Metcalf-Pate","doi":"10.1177/00236772231225896","DOIUrl":"10.1177/00236772231225896","url":null,"abstract":"","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lethal Gram-negative sepsis in healthy pigs during anaesthesia with contaminated propofol. 健康猪在使用受污染的异丙酚进行麻醉期间发生致命的革兰氏阴性败血症。
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-01 Epub Date: 2024-06-11 DOI: 10.1177/00236772231200524
Marit G Maaland, Marianne Oropeza-Moe, Silje K Nes, Frode Myrland, Cecilie Ersdal, Nils P Oveland
{"title":"Lethal Gram-negative sepsis in healthy pigs during anaesthesia with contaminated propofol.","authors":"Marit G Maaland, Marianne Oropeza-Moe, Silje K Nes, Frode Myrland, Cecilie Ersdal, Nils P Oveland","doi":"10.1177/00236772231200524","DOIUrl":"10.1177/00236772231200524","url":null,"abstract":"<p><p>Two healthy Landrace pigs anaesthetized with propofol suffered rapid onset of fatal sepsis. Clinical signs included severe arterial hypotension, loss of peripheral oxygenation, low end-tidal CO<sub>2</sub>, clinical onset of pulmonary oedema and cardiac dysfunction. Gross and histopathological examination revealed loss of vascular integrity with severe lung oedema and congestion, haemorrhages in several organs and fluid leakage into body cavities. Large numbers of Gram-negative bacteria, primarily <i>Klebsiella</i> sp., were present in the anaesthetic infusion containing propofol and were also cultured from internal organs of both pigs. The propofol was likely contaminated by bacteria after inappropriate handling and storage in the operating room. This report illustrates the potential for severe nosocomial infection when applying propofol in animals and humans and may serve as a reminder of the importance of strict aseptic practice in general, and specifically in the handling of this anaesthetic agent.</p>","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of a gel-based versus micro-pellet diet for adult zebrafish (Danio rerio). 评估成年斑马鱼（Danio rerio）的凝胶型和微颗粒型食物。
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-01 Epub Date: 2024-08-05 DOI: 10.1177/00236772241244834
Christine L Archer, Nikki K Tsuji, Molly H Waters, Jennifer L Brazzell, Derek L Fong, Jori K Leszczynski, Christopher A Manuel, Lauren M Habenicht
{"title":"Evaluation of a gel-based versus micro-pellet diet for adult zebrafish (<i>Danio rerio</i>).","authors":"Christine L Archer, Nikki K Tsuji, Molly H Waters, Jennifer L Brazzell, Derek L Fong, Jori K Leszczynski, Christopher A Manuel, Lauren M Habenicht","doi":"10.1177/00236772241244834","DOIUrl":"10.1177/00236772241244834","url":null,"abstract":"","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retrospective analysis on the occurrence of kidney cysts in mice in a central animal facility in the years 2009-2019. 2009-2019 年中央动物设施小鼠肾囊肿发生率的回顾性分析。
IF 1.3 4区 农林科学
Laboratory Animals Pub Date : 2024-08-01 Epub Date: 2024-08-05 DOI: 10.1177/00236772241242538
Katrin Becker
{"title":"Retrospective analysis on the occurrence of kidney cysts in mice in a central animal facility in the years 2009-2019.","authors":"Katrin Becker","doi":"10.1177/00236772241242538","DOIUrl":"10.1177/00236772241242538","url":null,"abstract":"<p><p>Kidney cysts in humans are mainly caused by inheritable polycystic kidney disease. Although they are a regular finding in laboratory mice, their occurrence upon dissection has not been systematically investigated, yet. Therefore, the aim of this report was to investigate on prevalence, phenotype and aetiology of spontaneously occurring kidney cysts in mice by retrospectively analysing the laboratory-receipt tables of the in-house laboratory of a central animal facility in North Rhine-Westphalia, Germany, years 2009-2019. A percentage of 0.4% of dissected mice displayed kidney cysts, with more male than female animals affected and average age equal to that of all dissected animals. Preliminary report in half of the cases was distended abdomen, and a few individuals displayed additional pathologic alterations of kidneys, most commonly dilated renal pelvis, or extrarenal comorbidities. Kidney cysts occurred independently of a renal phenotype of the transgenic strain or presence of infectious agents in health monitoring. To conclude, kidney cysts were characterized as harmless for affected mice but, as inheritability is suggested according with the literature, affected animals should be excluded from breeding.</p>","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信