Laboratory Animals最新文献_第4页

Response to Landes: Comment on Frommlet and Heinze (2021): correcting the error degrees of freedom. 对 Landes 的回应：对 Frommlet 和 Heinze (2021) 的评论：修正误差自由度。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-12-18 DOI: 10.1177/00236772241276822

Georg Heinze, Florian Frommlet

引用次数: 0

The effectiveness of educational workshops in improving the knowledge and attitude of attendees in laboratory animal science: A pre- and post-intervention design in Iran. 提高参与者在实验动物科学方面的知识和态度的教育讲习班的有效性：伊朗干预前后的设计。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-12-24 DOI: 10.1177/00236772221109996

Siavash Ahmadi-Noorbakhsh, Jila Sadighi, Zahra Hatami, Ehsan Shamsi Gooshki

{"title":"The effectiveness of educational workshops in improving the knowledge and attitude of attendees in laboratory animal science: A pre- and post-intervention design in Iran.","authors":"Siavash Ahmadi-Noorbakhsh, Jila Sadighi, Zahra Hatami, Ehsan Shamsi Gooshki","doi":"10.1177/00236772221109996","DOIUrl":"10.1177/00236772221109996","url":null,"abstract":"The knowledge and attitude of researchers can affect the validity of laboratory animal (LAN) research. However, studies show that not all researchers possess the required knowledge and appropriate attitude for performing valid research on animals. This may have several reasons, such as high heterogeneity in the educational backgrounds of researchers in animal trials. In this study, we hypothesized that properly designed intensive educational intervention could improve the knowledge and attitude of a mixed population of researchers, regardless of their heterogeneity. We delivered 10 country-wide two-day LAN workshops for academic members, postgraduate students and members of the ethics committees of the medical universities in Iran. Using pre-/post-intervention design, we found that the intervention highly significantly (p < 0.001) improved the knowledge score (from 2.96 ± 1.483 to 4.63 ± 1.548 mean ± SD; range: 0-8; n = 236) and attitude score (from 62.65 ± 5.160 to 65.57 ± 4.716; range: 14-70; N = 229). We also found that the educational intervention was significantly (p = 0.035) more effective to improve the knowledge of participants with more years of experience in LAN science. Unexpectedly, our younger participants (overall age range: 19-67 years old; mean ± SD: 37.96 ± 9.55) had lower initial attitude score. However, education was significantly (p = 0.002) more effective to improve the attitude score of younger participants. The knowledge and attitude improvements were not related to educational background, gender, history of prior attendance in LAN courses and amount of animal use. Attendees' performance in the final assessment showed that they require more education on the 'anesthesia/analgesia' topic. They also declared a high interest in learning more on the design of LAN studies.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"261-271"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decision trees for determining the fate of laboratory animals. 决定实验动物命运的决策树。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-10-08 DOI: 10.1177/00236772241271034

Myhailo Reshetnykov, Karen Stumm, Michael Zwick, Jan Baumgart

{"title":"Decision trees for determining the fate of laboratory animals.","authors":"Myhailo Reshetnykov, Karen Stumm, Michael Zwick, Jan Baumgart","doi":"10.1177/00236772241271034","DOIUrl":"10.1177/00236772241271034","url":null,"abstract":"Facilities involved in laboratory animal research often face ethical challenges such as: what should I do with the animals that are no longer suitable for experimental purposes? One of the common answers to this question is to kill them. And while numerous scientifically justifiable reasons exist for killing laboratory animals, we must not overlook our ethical responsibility towards these sentient beings. Animal facility managers and scientists frequently find themselves in a moral dilemma, torn between furthering their research and addressing the well-being of experimental animals required for their studies. We elaborated a concept consisting of six decision trees and recommendations for making informed decisions about the need to kill laboratory animals in research facilities, considering legal and ethical considerations. The concept is based on the German regulatory perspective. However, the measures and decisions for animal welfare can be implemented in all laboratory animal facilities. These recommendations suggest several courses of action, including implementing consistent breeding plans, exploring alternative uses, reassigning surplus animals and their organs, and establishing appropriate housing capacity limits that ensure species-appropriate care. We encourage scientists and animal facility managers to develop and implement decision-making frameworks and procedures tailored to their specific facilities, in the hope that this work will promote a thoughtful and responsible approach to the complex challenges associated with the killing of laboratory animals, advancing scientific progress and the humane treatment of these animals.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"157-168"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An adaptable, user-friendly score sheet to monitor welfare in experimental fish. 一个适应性强、用户友好的计分表，用于监测实验鱼的福利。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-12-12 DOI: 10.1177/00236772241271013

Mathilde Flueck-Giraud, Heike Schmidt-Posthaus, Alessandra Bergadano, Irene Adrian-Kalchhauser

{"title":"An adaptable, user-friendly score sheet to monitor welfare in experimental fish.","authors":"Mathilde Flueck-Giraud, Heike Schmidt-Posthaus, Alessandra Bergadano, Irene Adrian-Kalchhauser","doi":"10.1177/00236772241271013","DOIUrl":"10.1177/00236772241271013","url":null,"abstract":"Fish are increasingly used as experimental animals across research fields. Currently, around a quarter of all experimental animals used are fish. Less than 20% of these are standard model species. Welfare assessments for experimental fish are in their infancy compared with those for rodents. This can be attributed to the diversity of species used, the relative recency of fish as the go-to model for research, and challenges to assess welfare in non-vocal underwater species. The lack of guidelines and tools presents a challenge for researchers (particularly, for newcomers), for ethics committees and for implementing refinement measures. Here, we present an adaptable, user-friendly score sheet for fish based on MS Excel. The parameters are based on a literature review, have been validated by expert interviews and evaluated by a fish pathologist. The tool allows scoring of individual fish as well as groups, calculates summary scores and visualizes trends. We provide the underlying literature, give use examples and provide instructions on the adaptation and use of the score sheet. We hope that this tool will empower researchers to include welfare assessment in their routines, foster discussions on fish welfare parameters among scientists, facilitate interactions with ethics committees and, most importantly, enable the refinement of fish experiments.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"226-241"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pitfalls in the analysis conditions of canine brain perfusion computed tomography. 犬脑灌注计算机断层扫描分析条件的缺陷。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-12-18 DOI: 10.1177/00236772241280013

Kazuya Kushida, Kodai Tashiro, Masaaki Katayama, Ryuji Fukushima, Miori Kishimoto

{"title":"Pitfalls in the analysis conditions of canine brain perfusion computed tomography.","authors":"Kazuya Kushida, Kodai Tashiro, Masaaki Katayama, Ryuji Fukushima, Miori Kishimoto","doi":"10.1177/00236772241280013","DOIUrl":"10.1177/00236772241280013","url":null,"abstract":"This study aimed to investigate the impact of selected analysis conditions on blood flow values and color maps in canine brain perfusion computed tomography (PCT) and to propose optimal analysis conditions. Dynamic computed tomography imaging was performed on six beagle dogs. Color maps were generated using a combination of analysis algorithms (box-modulation transfer function (Box-MTF) and singular value deconvolution plus (SVD+) methods), slice thicknesses (4.0 and 8.0 mm), analysis matrix sizes (512 × 512, 256 × 256, and 128 × 128), and noise reduction levels (strong and weak). Cerebral blood flow (CBF) and cerebral blood volume (CBV) were calculated for gray matter, white matter, basal ganglia, hippocampus, thalamus, and cerebellum in each map. CBF and CBV values obtained using SVD+ were significantly higher than those obtained using Box-MTF. Noise reduction was more effective with larger matrix sizes; however, excessive noise reduction led to the blurring of anatomical structures in the color map. Across all analysis algorithms, anatomical structures were challenging to visualize at 8.0 mm. For canine brain PCT, it is essential to choose a straightforward algorithm that remains unaffected by circulatory velocity or intracranial bone structure. Given the brain's size, the slice thickness should be minimal, noise reduction level should be suitable for the targeted area, and matrix size should be maximized.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"253-260"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spontaneous hepatocellular and gastric tumours in African clawed frogs (Xenopus laevis). 非洲爪蛙（Xenopus laevis）的自发性肝细胞瘤和胃瘤。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-10-22 DOI: 10.1177/00236772241273060

Bernat Martí-García, Simon L Priestnall, Yolanda Saavedra Torres, Alejandro Suárez-Bonnet

{"title":"Spontaneous hepatocellular and gastric tumours in African clawed frogs (Xenopus laevis).","authors":"Bernat Martí-García, Simon L Priestnall, Yolanda Saavedra Torres, Alejandro Suárez-Bonnet","doi":"10.1177/00236772241273060","DOIUrl":"10.1177/00236772241273060","url":null,"abstract":"Three seven-year-old African clawed frogs (Xenopus laevis) from an animal research facility showed weight loss, coelomic distention and an abnormal swimming gait were euthanised on welfare grounds. A complete necropsy of each animal showed a focal, firm, dark brown to green mass with multifocal haemorrhages in the left liver lobe in two animals and a transmural, firm, beige, multilobulated gastric mass in another animal. Additionally, one of the frogs with the hepatic mass had haemocoeloma. Histologically, the hepatic masses were diagnosed as hepatocellular adenomas and the gastric mass as a gastric carcinoma with trans-coelomic metastases. The three tumours were immunophenotyped using the following antibodies: cytokeratin AE1/AE3, vimentin, E-cadherin, P53 and Ki67 and, additionally, for the hepatic tumours only, synaptophysin, Prox-1, S100 and Sox-9. Masson's trichrome, Periodic acid Schiff and Gram stains was also performed in selected cases. One of the hepatocellular adenomas was weakly positively labelled with E-cadherin whereas the other showed variable positivity for Sox-9 only. Vimentin labelled the stroma and sinusoidal endothelia. Interestingly in the liver, the cytokeratin AE1/AE3 labelling was restricted to the biliary epithelium and sinusoidal endothelia. The gastric carcinoma labelled positively with cytokeratin AE1/AE3 only. This report aims to guide laboratory animal veterinarians to accurately diagnose multi-organ masses in amphibians. To the authors' knowledge, this is the first comprehensive morphological study on a case series of hepatocellular and gastric neoplasia in X. laevis.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"290-297"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiorespiratory, hemodynamic, and sedative effects of dexmedetomidine in sheep. 右美托咪定对绵羊心肺功能、血液动力学和镇静作用的影响。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-11-20 DOI: 10.1177/00236772241277891

Janainne Hilbig Gomes, Isabela Plazza Bittar, Rauane Sousa de Moura, Bruna Ginú Prado, Leandro Guimarães Franco

{"title":"Cardiorespiratory, hemodynamic, and sedative effects of dexmedetomidine in sheep.","authors":"Janainne Hilbig Gomes, Isabela Plazza Bittar, Rauane Sousa de Moura, Bruna Ginú Prado, Leandro Guimarães Franco","doi":"10.1177/00236772241277891","DOIUrl":"10.1177/00236772241277891","url":null,"abstract":"An alternative in an attempt to minimize the effects triggered by intravenous (IV) bolus administration of α-2 adrenergic receptor agonists are continuous rate infusions (CRI). The requirement for sedation protocols in sheep for procedures to be performed without physical restraint and with reduced adverse effects, commonly observed with bolus use, justifies the study of CRI. The aim of study was to compare the cardiopulmonary and sedative effects of IV bolus injection and CRI of dexmedetomidine (DEX) in sheep. Six adult male sheep (38.3 ± 7.6 kg) received DEX as a bolus (5 µg kg-1, DEXbolus treatment) or CRI (5 µg kg-1 h-1 for 1 h, DEXCRI treatment). We recorded heartrate (HR), respiratory rate, systemic arterial blood pressure, pulse oximetry, hemodynamic parameters, blood gases and sedation scores over 120 min. HR was significantly lower in DEXbolus at 5 and 15 min than in DEXCRI, with HR reduction observed for 30 min in DEXbolus. Hypoxemia was noted in DEXbolus at 10 and 30 min. Pulmonary vascular resistance index increased at 5 min, and cardiac index (CI) decreased at all timepoints compared with baseline in DEXbolus. In DEXCRI, CI decreased only at 45 min. Sedation scores were higher in DEXbolus at 15 and 30 min. DEX CRI administration resulted in fewer cardiorespiratory and hemodynamic changes compared with bolus injection and lower sedation scores (<4/10), which would not allow animal handling without a reaction. The load dose used in CRI was a limitation to constant infusion.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"242-252"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Directive 2010/63/EU on research on wild-sourced animals: a review of publication trends in studies on captive wild mice (Apodemus sp.). 指令2010/63/EU对野生源动物研究的影响：圈养野生小鼠（Apodemus sp.）研究的发表趋势综述

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-12-12 DOI: 10.1177/00236772241290404

Maílis Carrilho, Sophie von Merten, Maria da Luz Mathias, Joaquim T Tapisso

{"title":"Effects of Directive 2010/63/EU on research on wild-sourced animals: a review of publication trends in studies on captive wild mice (Apodemus sp.).","authors":"Maílis Carrilho, Sophie von Merten, Maria da Luz Mathias, Joaquim T Tapisso","doi":"10.1177/00236772241290404","DOIUrl":"10.1177/00236772241290404","url":null,"abstract":"Most animals used in experimentation are small mammals. In the EU, Directive 2010/63/EU regulates the use of laboratory animals for experimental purposes. However, there are few guidelines for the use of wild-sourced animals, which cover permits, experimentation, transport, maintenance, and setting free after experiments. To evaluate the effect of the Directive on the study of wild-sourced animals, we conducted a systematised literature review focusing on three widespread rodents of the genus Apodemus: Apodemus agrarius, A. flavicollis and A. sylvaticus. We selected studies performed across the EU, published before (2000-August 2010), during (September 2010-2012) and after implementation of the Directive (2013-2022). From those, we collected data on three main topics: i) authorisation; ii) care and accommodation and iii) methods of killing. We found that after implementation of the Directive a higher proportion of published studies provided information about authorisation. In contrast, there was no significant difference over time in the information given about the care and accommodation of animals or methods of killing. As such, our analysis suggests that there is still room for improvement to achieve consistency across journals publishing research involving wild-sourced small mammals. Specifically, editors should require the provision of detailed information by authors regarding proper animal care (e.g. more detailed care and accommodation protocols). To harmonise the information requested by different editorial boards, we recommend the addition of specific guidelines in the Directive regarding wild animals, particularly on their proper accommodation, manipulation, enrichment and veterinary control.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"145-156"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Animal researchers' views on the publication of negative results and subsequent policy adoptions. 动物研究人员对公布负面结果的看法以及随后采取的政策。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-10-24 DOI: 10.1177/00236772241271039

Carlos Oscar S Sorzano, Daniel G Hamilton, Eva Mendez

引用次数: 0

Extended oxygen supplementation after thoracotomy in rats may improve welfare. 大鼠胸廓切开术后延长氧气补充时间可改善福利。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-11-04 DOI: 10.1177/00236772241273061

Bryony Few, Alex Dugdale

引用次数: 0