Laboratory Animals最新文献_第4页

Establishment of reference intervals of haematology and biochemistry analytes in ICR mice of different ages. 确定不同年龄 ICR 小鼠血液学和生物化学分析指标的参考区间。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-09-30 DOI: 10.1177/00236772241260909

Suresh Patel, Satish Patel, Ashvin Kotadiya, Samir Patel, Bhavesh Shrimali, Tushar Patel, Harshida Trivedi, Vishal Patel, Jogeswar Mahapatra, Mukul Jain

{"title":"Establishment of reference intervals of haematology and biochemistry analytes in ICR mice of different ages.","authors":"Suresh Patel, Satish Patel, Ashvin Kotadiya, Samir Patel, Bhavesh Shrimali, Tushar Patel, Harshida Trivedi, Vishal Patel, Jogeswar Mahapatra, Mukul Jain","doi":"10.1177/00236772241260909","DOIUrl":"10.1177/00236772241260909","url":null,"abstract":"Outbred stocks of mice are widely used in pre-clinical research as these animals possess a diversified genetic background when compared with inbred strains of mice. It is crucial to assess particular alterations in the physiological and functional profiles of laboratory animals using haematological and biochemical indicators. These values can also differ between laboratories because they are influenced by many different factors. We aimed to provide normal values and reference intervals for selected haematology and biochemistry analytes of 570 ICR mice at three different ages: 6-8 weeks, 10-14 weeks and 6-9 months. Reference values were calculated by non-parametric methods. For comparisons between sexes, the independent-sample t-test and Mann-Whitney test were employed, and analysis of variance was used for age differences. The findings of the study revealed age-related declines in haemoglobin concentration, haematocrit, mean corpuscular volume and mean corpuscular haemoglobin concentrations. Mice aged 6-9 months had statistically higher platelet counts in their blood than mice of other ages. The white blood cell count had a significant age effect and progressively decreased with age. As mice get older, the percentage of neutrophils, monocytes and basophils increases, but the percentage of lymphocytes decreases. For the biochemical values, age-related significant differences in glucose, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and albumin concentrations were found. It was also found that creatinine concentrations were comparable across all age ranges. The values presented in the present work can be used as a reference to interpret clinical pathology data for other studies and to evaluate health status.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"565-578"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of two bedding types on the breeding fecundity of C57BL/6J mice. 两种垫料对 C57BL/6J 小鼠繁殖力的影响比较

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI: 10.1177/00236772241249783

Jessica L Pinckard, Lauren A Gibson, Wendy R Williams

{"title":"Comparison of two bedding types on the breeding fecundity of C57BL/6J mice.","authors":"Jessica L Pinckard, Lauren A Gibson, Wendy R Williams","doi":"10.1177/00236772241249783","DOIUrl":"10.1177/00236772241249783","url":null,"abstract":"One of the favored options for generating complex transgenic laboratory mice is through in-house breeding and management strategies. One consideration in the management of these colonies is how the animals' environment may affect reproductive success. Several aspects of the microenvironment can be controlled or manipulated, including cage type, bedding, enrichment, diet, and temperature and humidity. This study sought to evaluate reproductive outcomes for C57BL/6J mice that were randomly assigned to one of two different bedding types: paper based or corncob bedding. Our hypothesis was there would be no significant difference in reproductive outcomes between the two bedding types. A total of 10 males and 10 females were paired at 45 days of age. Animals were allowed to breed for 15 consecutive weeks. Cages were checked daily for the presence of pups and a pup count was performed at 7 days of age. Weaning occurred at 20 or 21 days of age, at which time a final pup count, pup weight, and sex were recorded. All litters born and pups weaned in the 15-week timeframe were used for data analysis. Statistical analysis compared cannibalization between the two groups and the results showed no statistical difference between groups (p > 0.05). Other parameters analyzed included average litter size, average weaning weight, and number of litters per group. All pups counted at Day 7 survived to weaning age in both groups. We concluded that both bedding types produced similar success regarding breeding fecundity in C57BL/6J mice.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"545-551"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The implementation of tunnel handling in a mouse breeding facility revealed strain-specific behavioural responses. 在小鼠育种设施中实施隧道处理后，发现了特定品系的行为反应。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2023-12-23 DOI: 10.1177/00236772231215077

Katharina Hohlbaum, Roswitha Merle, Ramona Warnke, Stefan Nagel-Riedasch, Christa Thöne-Reineke, Kristina Ullmann

{"title":"The implementation of tunnel handling in a mouse breeding facility revealed strain-specific behavioural responses.","authors":"Katharina Hohlbaum, Roswitha Merle, Ramona Warnke, Stefan Nagel-Riedasch, Christa Thöne-Reineke, Kristina Ullmann","doi":"10.1177/00236772231215077","DOIUrl":"10.1177/00236772231215077","url":null,"abstract":"As a step towards implementing non-aversive handling techniques at a big mouse breeding facility in Germany, tunnel handling was introduced in a breeding unit comprising three inbred mouse strains. To assess whether tunnel handling would be feasible for the animal technicians in their everyday work and beneficial for the mice when being handled during weekly cage change only, the behaviour of tunnel- and tail-handled animals of both sexes was examined before, during and after the handling events over a period of nine weeks. Moreover, the time expenditure was compared between both handling techniques. It was possible to use the tunnel in all three mouse strains. However, the impact of the handling techniques on the behavioural parameters investigated in the present study were strain-specific. All behavioural parameters suggested that NZW mice benefited the most from tunnel handling. The results obtained from Hello Kitty and WNK mice were ambiguous, which may suggest that a brief handling session during the cage clean may have not been sufficient to habituate them to the process of handling. It took the animal technicians approximately 3 seconds longer per mouse when using a tunnel. The strain-specific results should encourage researchers to share their experiences with non-aversive handling techniques in different mouse strains, for example, along with their research articles.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"552-564"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spontaneous type II cell-derived bronchioloalveolar adenoma in a young ICR mouse. 一只幼年 ICR 小鼠自发性 II 型细胞衍生支气管肺泡腺瘤。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1177/00236772241257726

Minkyoung Sung, Soo-Eun Sung, Joo-Hee Choi, Yujeong Lee, Kyung-Ku Kang, SunHee Park, Duyeol Kim, Sijoon Lee

引用次数: 0

Refining urine collection in mice: Development of an innovative urine collection device. 改进小鼠的尿液收集：开发创新型尿液收集装置

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI: 10.1177/00236772231219828

Mélodie Douté, Céline Monzali, Antonino Nicoletti, Giuseppina Caligiuri, Marc Clement

引用次数: 0

Cardiorespiratory, hemodynamic, and sedative effects of dexmedetomidine in sheep. 右美托咪定对绵羊心肺功能、血液动力学和镇静作用的影响。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-11-20 DOI: 10.1177/00236772241277891

Janainne Hilbig Gomes, Isabela Plazza Bittar, Rauane Sousa de Moura, Bruna Ginú Prado, Leandro Guimarães Franco

{"title":"Cardiorespiratory, hemodynamic, and sedative effects of dexmedetomidine in sheep.","authors":"Janainne Hilbig Gomes, Isabela Plazza Bittar, Rauane Sousa de Moura, Bruna Ginú Prado, Leandro Guimarães Franco","doi":"10.1177/00236772241277891","DOIUrl":"https://doi.org/10.1177/00236772241277891","url":null,"abstract":"An alternative in an attempt to minimize the effects triggered by intravenous (IV) bolus administration of α-2 adrenergic receptor agonists are continuous rate infusions (CRI). The requirement for sedation protocols in sheep for procedures to be performed without physical restraint and with reduced adverse effects, commonly observed with bolus use, justifies the study of CRI. The aim of study was to compare the cardiopulmonary and sedative effects of IV bolus injection and CRI of dexmedetomidine (DEX) in sheep. Six adult male sheep (38.3 ± 7.6 kg) received DEX as a bolus (5 µg kg-1, DEXbolus treatment) or CRI (5 µg kg-1 h-1 for 1 h, DEXCRI treatment). We recorded heartrate (HR), respiratory rate, systemic arterial blood pressure, pulse oximetry, hemodynamic parameters, blood gases and sedation scores over 120 min. HR was significantly lower in DEXbolus at 5 and 15 min than in DEXCRI, with HR reduction observed for 30 min in DEXbolus. Hypoxemia was noted in DEXbolus at 10 and 30 min. Pulmonary vascular resistance index increased at 5 min, and cardiac index (CI) decreased at all timepoints compared with baseline in DEXbolus. In DEXCRI, CI decreased only at 45 min. Sedation scores were higher in DEXbolus at 15 and 30 min. DEX CRI administration resulted in fewer cardiorespiratory and hemodynamic changes compared with bolus injection and lower sedation scores (<4/10), which would not allow animal handling without a reaction. The load dose used in CRI was a limitation to constant infusion.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241277891"},"PeriodicalIF":1.3,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of zoonoses in research institutions - lessons learned from a Coxiella burnetii outbreak case. 研究机构的人畜共患病管理--从一例烧伤柯西氏菌爆发中汲取的教训。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-11-19 DOI: 10.1177/00236772241271028

Pia Ks Ostach, André Dülsner, Anne Keil, Stefan Nagel-Riedasch

{"title":"Management of zoonoses in research institutions - lessons learned from a Coxiella burnetii outbreak case.","authors":"Pia Ks Ostach, André Dülsner, Anne Keil, Stefan Nagel-Riedasch","doi":"10.1177/00236772241271028","DOIUrl":"10.1177/00236772241271028","url":null,"abstract":"When biomedical research investigates the human surgical situation in the need of a chronic course, it is more often possible to do so using large animal models. The use of farm animals always poses special challenges for the institution conducting the research in terms of infection prevention and occupational safety. Especially for the zoonotic disease coxiellosis it is important to be aware of the constant risk of pathogen introduction by small ruminants and to take appropriate precautions. In this way, personal injury should be avoided or at least be kept to a minimum in the event of infection since then sustainable zoonosis control can be immediately initiated. Using the example of a Q fever outbreak at a research facility, we want to share with this extended case report the importance of central emergency structures, provisions and the inclusion of relevant experts and disciplines in a crisis team. Its primary purpose is to support the affected facility and coordinate the implementation of necessary cleaning, disinfection and decontamination measures in close contact with the responsible local authorities. The aim is to inactivate the pathogen in a systematic and controlled manner in few steps of action only and to keep the interruption of the facility's operations as short as possible.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241271028"},"PeriodicalIF":1.3,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extended oxygen supplementation after thoracotomy in rats may improve welfare. 大鼠胸廓切开术后延长氧气补充时间可改善福利。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-11-04 DOI: 10.1177/00236772241273061

Bryony Few, Alex Dugdale

{"title":"Extended oxygen supplementation after thoracotomy in rats may improve welfare.","authors":"Bryony Few, Alex Dugdale","doi":"10.1177/00236772241273061","DOIUrl":"https://doi.org/10.1177/00236772241273061","url":null,"abstract":"A retrospective comparison of welfare indicators in male rats undergoing thoracotomy for intrapleural dosing is presented. The initial cohort (n = 7) breathed room air after recovery from anaesthesia, while later cohorts (n = 12) had oxygen supplementation for up to 48 h post-surgery. Rats breathing room air sustained a statistically significant average body-weight loss of -1.62% (±1.7%) 2 days after surgery, compared with rats given oxygen supplementation, which maintained a mean weight gain of 0.87% (±1.75%) (p = 0.009). Oxygen-supplemented rats also had lower pain scores on the evening after surgery (median 0.075 [range 0-1.75] vs. median 1.5 [range 0.5-2]). This difference was not statistically significant (p = 0.063) but may be of clinical significance. All rats displayed transient sedation after post-operative opioid administration on the day of surgery, and hypoxaemia (SpO2 <90%) was observed in rats maintained on room air. Use of an oxygen concentrator to provide post-operative extended oxygen supplementation was easy to implement and may improve animal welfare post-thoracotomy.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241273061"},"PeriodicalIF":1.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An innovative approach for health and safety training and occupational health program annual enrollment for laboratory animal care and use personnel. 为实验室动物护理和使用人员提供健康与安全培训和职业健康计划年度注册的创新方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-11-04 DOI: 10.1177/00236772241263903

Scott J Patlovich, Robert J Emery, Kristin G King, Zackary B Becker, Rebecca B Kairis, Mary A Robinson, Joy M Harrison, George Delclos, William B Perkison

{"title":"An innovative approach for health and safety training and occupational health program annual enrollment for laboratory animal care and use personnel.","authors":"Scott J Patlovich, Robert J Emery, Kristin G King, Zackary B Becker, Rebecca B Kairis, Mary A Robinson, Joy M Harrison, George Delclos, William B Perkison","doi":"10.1177/00236772241263903","DOIUrl":"https://doi.org/10.1177/00236772241263903","url":null,"abstract":"Animal care and use personnel in research laboratory facilities are inherently exposed to a variety of workplace hazards. The health and safety of the workforce working directly with or around research animals is of paramount importance, and as such, an occupational health and safety program for at-risk staff is essential. In order to maximize participation in and the effectiveness of health and safety training and occupational health program enrollment for animal care and use personnel at an academic health sciences university, an innovative annual \"health fair\" was developed and implemented at The University of Texas Health Science Center at Houston. This event allows personnel working in positions that present exposure risk to research animals to be able to obtain hazard-specific health and safety training, re-enroll in the occupational health oversight program and update their medical history, and participate in the institution's other safety programs as required by job title or assigned job tasks. By providing a comprehensive health fair that offers convenient access to the necessary safety training and occupational health services in one location during a designated period of time, management and staff are incentivized to participate and have reported satisfaction with and appreciation of the convenient access. Summarized here is how we plan, organize, and effectively execute the annual health fair so that other institutions who might wish to use this strategy can learn from our approach.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241263903"},"PeriodicalIF":1.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Animal researchers' views on the publication of negative results and subsequent policy adoptions. 动物研究人员对公布负面结果的看法以及随后采取的政策。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-24 DOI: 10.1177/00236772241271039

Carlos Oscar S Sorzano, Daniel G Hamilton, Eva Mendez

引用次数: 0