Laboratory Animals最新文献_第4页

Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science. 建立桥梁：动物护理人员和实验室技术人员参与实验计划和动物研究的实施，以提高工作满意度和科学水平。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-06-01 Epub Date: 2026-04-14 DOI: 10.1177/00236772251400976

Fernando Gonzalez-Uarquin, Paulin Jirkof, Bettina Bert, Penny Hawkins, Ljupco Angelovski, Jan Baumgart, Nadine Baumgart, Özge S Cevik, Nuno H Franco, Erdal Horata, Rohish Kaura, Winfried Neuhaus, Brigida Riso, Adrian J Smith, Athanassia Sotiropoulos, Augusto Vitale, Sophie Schober

{"title":"Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science.","authors":"Fernando Gonzalez-Uarquin, Paulin Jirkof, Bettina Bert, Penny Hawkins, Ljupco Angelovski, Jan Baumgart, Nadine Baumgart, Özge S Cevik, Nuno H Franco, Erdal Horata, Rohish Kaura, Winfried Neuhaus, Brigida Riso, Adrian J Smith, Athanassia Sotiropoulos, Augusto Vitale, Sophie Schober","doi":"10.1177/00236772251400976","DOIUrl":"10.1177/00236772251400976","url":null,"abstract":"The involvement of non-scientific staff in discussions about animal welfare and scientific quality is essential for biomedical research progress. In this study, we developed a survey to collect the self-perception of animal care staff (ACS) and laboratory technicians about their involvement in scientific planning and conduct. Participants were contacted to complete an anonymous online questionnaire. We obtained 850 responses, mainly from Europe: 564 from ACS and 286 from laboratory technicians. Job satisfaction was assessed as positive by ACS and laboratory technicians despite the low frequency of culture of care activities and mental health meetings. Both groups expressed their desire to be trained in research planning and conduct; however, regular training was not reported. In addition, the inability to act on animal welfare concerns owing to experimental reasons was reported by both groups. Over half of the participants felt valued and appreciated by the lead scientists or animal facility manager; however, it is not clear how they are acknowledged, as their names on the authors list or in the manuscript acknowledgments are barely included. Our results indicated that involvement of ACS and laboratory technicians in planning and conducting studies would improve their understanding of how experiments are done, and therefore communication processes, work satisfaction, animal welfare, and scientific quality. Finally, we provided recommendations to improve the engagement of ACS and laboratory technicians in discussions about animal research planning and conduct.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"318-331"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13373277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaesthesia for pigs, sheep, goats and cattle involved in biomedical research: FELASA working group guidelines-Parts I-IV. 参与生物医学研究的猪、绵羊、山羊和牛的麻醉：FELASA工作组指南-第I-IV部分。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-06-01 Epub Date: 2026-06-11 DOI: 10.1177/00236772261442027

Sabine J Bischoff, Gabrielle C Musk, Alenka Seliškar, Daniela Casoni, Stéphanie De Vleeschauwer, R Eddie Clutton

{"title":"Anaesthesia for pigs, sheep, goats and cattle involved in biomedical research: FELASA working group guidelines-Parts I-IV.","authors":"Sabine J Bischoff, Gabrielle C Musk, Alenka Seliškar, Daniela Casoni, Stéphanie De Vleeschauwer, R Eddie Clutton","doi":"10.1177/00236772261442027","DOIUrl":"10.1177/00236772261442027","url":null,"abstract":"Recommendations for safe anaesthesia are common in medical practice, have been formulated for traditional laboratory species, i.e. rodents, but do not exist for laboratory pigs, sheep, goats and cattle. The guidelines presented here were commissioned by the Federation of European Laboratory Animal Science Associations (FELASA) and serve to underscore EU Directive 2010/63/EU (Article 14) which require that, 'procedures are carried out with general or local anaesthesia and analgesia or another appropriate method to ensure pain, suffering and distress are kept to a minimum'. The guidelines are based on a review of: (i) medical and veterinary medical guidelines promoting safe anaesthetic practice; (ii) scientific literature pertaining to anaesthesia and analgesia in pigs, sheep, goats and cattle; and (iii) a consideration of the ethical, legal and scientific requirements when anaesthetizing laboratory animals. The guidelines aim to make recommendations for the provision of safe, practical and effective anaesthesia and analgesia to laboratory pigs and ruminants. Recommended standards for sedation and restraint (I), general principles of anaesthesia (II), monitoring anaesthesia (III) and pain assessment (IV) in the same species have been described by this working group (WG) and are set out in four parts in this document.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"346-396"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13373299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caring, killing and an always busy schedule: a cross-sectional study on stress and strain in laboratory animal professionals in German-speaking countries. 照顾、杀戮和总是忙碌的日程：德语国家实验动物专业人员压力和紧张的横断面研究。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-06-01 Epub Date: 2026-04-26 DOI: 10.1177/00236772261417391

Sonja Rumpel, Regina Kempen, Roswitha Merle, Christa Thöne-Reineke

{"title":"Caring, killing and an always busy schedule: a cross-sectional study on stress and strain in laboratory animal professionals in German-speaking countries.","authors":"Sonja Rumpel, Regina Kempen, Roswitha Merle, Christa Thöne-Reineke","doi":"10.1177/00236772261417391","DOIUrl":"10.1177/00236772261417391","url":null,"abstract":"Various studies have documented psychological strain in laboratory animal professionals (LAPs). However, reliable data on relevant stressors remain limited. This study aimed to assess potential stressors experienced by LAPs in German-speaking countries and analyse their impact on psychological strain. An online survey was conducted in summer 2023, assessing the participants' mental strain using the General Health Questionnaire (GHQ-12), common general stressors using the Copenhagen Psychosocial Questionnaire (COPSOQ), as well as potential job-specific stressors. The final sample included 932 anonymous LAP participants. Data were analysed descriptively and using hierarchical regression analysis. Tasks like the euthanasia of surplus animals or being unable to alleviate animal distress due to scientific needs emerged as job-specific stressors. However, the results of the hierarchical regression indicated that general stressors, such as work-privacy conflict, physical and emotional demands, the need to hide emotions, and the degree of one's own influence at work, explained at least as much of the variance in psychological strain as the specific stressors. In addition, work experience, function, and gender significantly influenced the GHQ score. The results from this study could contribute to fostering a culture of care by developing general protection means for the psychological hazards in the workplaces of LAPs, thus reducing their adverse effects on personnel, laboratory animals and research results.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"305-317"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13373282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring ethical reasoning and welfare perceptions of Octopus vulgaris paralarvae among scientific and non-scientific communities. 科学与非科学社群对章鱼幼虫的伦理推理与福利认知之探讨。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-06-01 Epub Date: 2026-05-27 DOI: 10.1177/00236772261441121

Jill Monnissen, Andreas De Block

{"title":"Exploring ethical reasoning and welfare perceptions of Octopus vulgaris paralarvae among scientific and non-scientific communities.","authors":"Jill Monnissen, Andreas De Block","doi":"10.1177/00236772261441121","DOIUrl":"10.1177/00236772261441121","url":null,"abstract":"Live cephalopods used in scientific research are protected under European Directive 2010/63/EU due to growing evidence that adult cephalopods can experience pain. This protection also extends to paralarval Octopus vulgaris, despite the absence of evidence that these early life stages are capable of nociception or pain. Consequently, the question arises whether welfare protection should be applied as a precaution when evidence of sentience is lacking, or only once it has been empirically demonstrated. Ethical reasoning under such uncertainty is influenced by views about sentience, scientific necessity, and societal relevance, and prior work suggests these views differ between scientists and laypeople. However, it remains unclear how these considerations combine when evidence for sentience is incomplete. This study investigated what influences ethical reasoning about the use of O. vulgaris paralarvae in research. An online survey (N = 205) presented cephalopod biologists, other biologists, and non-biologists with one of three scenarios describing a gene expression experiment on O. vulgaris paralarvae in which nociception-related genes were expressed, not expressed, or not yet investigated. The provided scientific information did not significantly affect ethical judgments, but beliefs about pain capacity, disciplinary background, and gender did. These findings suggest that ethical evaluations in the context of uncertain biological evidence are driven more by prior beliefs, disciplinary background, and gender than by scientific information and familiarity with research ethics. This underscores the need for more accessible science communication, improved ethical engagement, and more objective evaluation of ethics education to support well-informed and inclusive decision-making in animal research governance.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"294-304"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of adhesion scoring performance between humans and large language models in experimental rat laparotomy. 实验大鼠剖腹手术中人类与大型语言模型粘连评分的比较。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-06-01 Epub Date: 2026-04-08 DOI: 10.1177/00236772261426654

Sıtkıcan Okur, Esra Modoğlu, Büşra Baykal, Latif E Yanmaz, Esra M Kirman, Ayşe G Bedir, Damla T Okur, Yasemin Akçora

{"title":"Comparison of adhesion scoring performance between humans and large language models in experimental rat laparotomy.","authors":"Sıtkıcan Okur, Esra Modoğlu, Büşra Baykal, Latif E Yanmaz, Esra M Kirman, Ayşe G Bedir, Damla T Okur, Yasemin Akçora","doi":"10.1177/00236772261426654","DOIUrl":"10.1177/00236772261426654","url":null,"abstract":"This study compared the macroscopic adhesion scoring performance of large language models (LLMs: ChatGPT-o3, ChatGPT-5, Gemini-2.5 Pro) with that of novice veterinary surgeons, using expert consensus as the reference. Eighty standardized postoperative laparotomy cases in Wistar rats were photographed and scored using the Nair 0-4 adhesion scale. Two novice surgeons and three LLMs independently evaluated each case; the expert reference was defined by a surgeon and a pathologist. Group differences were analyzed using the Kruskal-Wallis test with Dunn-Bonferroni post hoc comparisons, correlations by Bonferroni-adjusted Spearman coefficients, human interobserver reliability by intraclass correlation coefficient (ICC) (A,1), and agreement with the expert by quadratic-weighted Cohen's κ and exact-match accuracy. Overall differences were significant. ChatGPT-o3, ChatGPT-5, Gemini-2.5 Pro, and Novice 1 assigned lower scores, while Novice 2 assigned higher scores. Correlations with the expert were significant for Novice 1 (ρ = 0.706), Novice 2 (ρ = 0.593), and ChatGPT-o3 (ρ = 0.617), but not for ChatGPT-5 or Gemini-2.5 Pro. Inter-observer reliability among human raters was moderate (ICC = 0.55). Importantly, absolute exact-match accuracies were modest across all evaluators, with the highest accuracy observed for Novice 1 (33.8%) and ⩽26.3% for the LLMs. While novices outperformed the models, these findings highlight the intrinsic difficulty of fine-grained Nair 0-4 adhesion scoring on two-dimensional intraoperative images and indicate that current LLMs are better suited as calibrated decision-support tools rather than stand-alone raters.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"258-269"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population-genomic stability of a closed-herd GGTA1-knock-out miniature pig line for xenotransplantation. 用于异种移植的封闭群ggta1敲除小型猪系的群体基因组稳定性。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-06-01 Epub Date: 2026-04-08 DOI: 10.1177/00236772261432004

Won Kil Lee, Sang Eun Kim, Dongwon Seo, Seunghoon Lee, Jin-Gu No, Seokho Kim, Min Hwa Do, Joo Young Lee, Namwoong Hyeong, Hwi-Cheul Lee, Poongyeon Lee, Keon Bong Oh

{"title":"Population-genomic stability of a closed-herd GGTA1-knock-out miniature pig line for xenotransplantation.","authors":"Won Kil Lee, Sang Eun Kim, Dongwon Seo, Seunghoon Lee, Jin-Gu No, Seokho Kim, Min Hwa Do, Joo Young Lee, Namwoong Hyeong, Hwi-Cheul Lee, Poongyeon Lee, Keon Bong Oh","doi":"10.1177/00236772261432004","DOIUrl":"10.1177/00236772261432004","url":null,"abstract":"Genetically engineered pigs are essential donors for xenotransplantation, requiring phenotypic stability and genetic definition. We evaluated the newly established GGTA1-knock-out \"XENO\" line, maintained as a closed herd for >10 generations, against Massachusetts General Hospital (MGH) miniature swine, commercial Landrace (LR), and Yorkshire × Landrace (Y × L) populations. One hundred and thirty-nine pigs were genotyped using an 80K SNP BeadChip. Morphometric monitoring at 18 months showed no significant differences in body weight, length, height, or heart girth between XENO and MGH pigs (all p > 0.05). Principal component and phylogenetic analyses separated the four groups into distinct genetic clusters, confirming the uniqueness of the XENO line. Chromosome-wide linkage disequilibrium was markedly higher in XENO (initial r2 > 0.8; half-decay ≈ 50 kb) than in commercial lines, reflecting intensive inbreeding. Linkage disequilibrium-derived historical effective population size (Ne) in XENO was approximately 1.5-fold lower than in LR/Y × L, but comparable to MGH. ADMIXTURE analysis supported K = 3 ancestral components with <2% introgression into XENO. These findings demonstrate that closed-herd management preserves phenotypic uniformity while establishing a genetically homogeneous, independent donor line. XENO pigs exhibited overlapping growth trajectories with MGH animals, providing a genomically stable resource for preclinical xenotransplantation.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"270-279"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A framework for assessing practical competence in laboratory animal science (LAS): development of direct observation of procedural skills (DOPS) by the ETPLAS Working Group. 评估实验动物科学（LAS）实践能力的框架：ETPLAS工作组对程序技能（DOPS）直接观察的发展。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-06-01 Epub Date: 2026-05-06 DOI: 10.1177/00236772261436913

Rafael Frías Beneyto, Ivo Tiebosch, Andreia Costa, Daniel Kylmann Hansen, Åsa Holmberg, Ronald Vlasbom, Lucy Whitfield, Anne-Dominique Degryse, Jan-Bas Prins

{"title":"A framework for assessing practical competence in laboratory animal science (LAS): development of direct observation of procedural skills (DOPS) by the ETPLAS Working Group.","authors":"Rafael Frías Beneyto, Ivo Tiebosch, Andreia Costa, Daniel Kylmann Hansen, Åsa Holmberg, Ronald Vlasbom, Lucy Whitfield, Anne-Dominique Degryse, Jan-Bas Prins","doi":"10.1177/00236772261436913","DOIUrl":"10.1177/00236772261436913","url":null,"abstract":"Assessment of practical skills is a key component in ensuring competence in laboratory animal science (LAS), particularly for practitioners performing functions A, C and D as defined in the Directive 2010/63/EU, namely those who carry out procedures on animals, care for them and perform humane killing, respectively. To address the need for a harmonised, objective and transparent approach to assessing practical competence, Working Group 4 (WG4) of the Education and Training Platform for Laboratory Animal Science (ETPLAS) has developed a set of Direct Observation of Procedural Skills (DOPS) tools. These tools are tailored specifically to evaluate the intended learning outcomes of Modules 3.2, 6.2, 8 and 20-22 of the European Union (EU) Education and Training Framework. This report outlines the rationale, structure and guidance for implementing the DOPS tools. Based on Miller's pyramid and established educational principles, DOPS tools are suitable for formative and summative assessments in simulated or real environments. They can be used both formatively to monitor progress during training and summatively to support end-of-training decisions about competence. Their implementation is crucial to supporting harmonised training, facilitating mutual recognition of competence and ultimately advancing both animal welfare and the quality of scientific research in the EU and beyond. The DOPS forms are available as supplemental material to this article and from the ETPLAS website.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"332-345"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing the 3Rs? Researchers' perspectives on institutional facilitation in Switzerland - Part 1: originative facilitation. 推进3r ？瑞士制度便利研究视角——第一部分：创新便利。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-19 DOI: 10.1177/00236772261436225

Edwin Louis-Maerten, Lester D Geneviève, Love Hansell, Charlotte E Blattner, Bernice S Elger

{"title":"Advancing the 3Rs? Researchers' perspectives on institutional facilitation in Switzerland - Part 1: originative facilitation.","authors":"Edwin Louis-Maerten, Lester D Geneviève, Love Hansell, Charlotte E Blattner, Bernice S Elger","doi":"10.1177/00236772261436225","DOIUrl":"10.1177/00236772261436225","url":null,"abstract":"The implementation of the 3R principles (Replace, Reduce, Refine) requires a comprehensive understanding of the various levels at which they operate, as well as the factors that may hinder or support their advancement. Central to this process is incorporating the perspectives of stakeholders in biomedical research to develop practical and relevant strategies for action. In a qualitative study, we conducted 35 semi-structured interviews with Swiss researchers working on the 3Rs or animal experimentation. Interviews were analysed following a reflexive thematic analysis, and we identified two categories of facilitation: originative facilitation, which concerns epistemic aspects of the 3Rs, and executive facilitation, which concerns interventionist aspects of the 3Rs. In this article, we report on the former dimension, which can be further divided into the conceptualisation of the 3Rs on the one hand, and the education of researchers on the other hand. Although grounded in the Swiss context, these findings reveal key insights for researchers, policymakers, and institutional stakeholders internationally. As efforts to align animal research practices with ethical standards continue to grow globally, understanding how the 3Rs are conceptualised and taught is essential for creating coherent, cross-border strategies that foster responsible scientific practice. Clarifying definitions and reinforcing researcher education are critical steps in ensuring the consistent and meaningful implementation of the 3Rs across national boundaries.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772261436225"},"PeriodicalIF":1.3,"publicationDate":"2026-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Support for 2025 ESLAV-ECLAM Summer School 'The art and science of surgical technique and experimental design'. 支持2025年ESLAV-ECLAM暑期学校“外科技术和实验设计的艺术和科学”。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2026-05-26 DOI: 10.1177/00236772261448456

Lal Secretariat

引用次数: 0

Carprofen increases the risk of anastomotic leakage in a mouse model of Roux-en-Y gastric bypass. 卡洛芬增加Roux-en-Y胃旁路术小鼠模型吻合口漏的风险。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2025-12-26 DOI: 10.1177/00236772251391394

Philip Claus, Rebecca Springer, Christoph Otto, Florian Seyfried, Nicolas Schlegel

{"title":"Carprofen increases the risk of anastomotic leakage in a mouse model of Roux-en-Y gastric bypass.","authors":"Philip Claus, Rebecca Springer, Christoph Otto, Florian Seyfried, Nicolas Schlegel","doi":"10.1177/00236772251391394","DOIUrl":"10.1177/00236772251391394","url":null,"abstract":"Anastomotic leakage following intestinal surgery in a murine Roux-en-Y gastric bypass (RYGB) model is usually fatal. Carprofen, a commonly used non-steroidal anti-inflammatory drug (NSAID) for postoperative analgesia in rodents, has been linked to negative effects on intestinal wound healing. The postoperative use of the non-opioid analgesic metamizole in this setting is rare. Here, we compared the potential effects of carprofen and metamizole on intestinal anastomotic healing in a murine RYGB model in order to optimize perioperative care in this animal model. A total of 38 mice underwent RYGB surgery as part of an in-house surgical training programme and received either carprofen (n = 17) or metamizole (n = 21) postoperatively. Within three postoperative days, anastomotic leakage occurred in 82% of carprofen-treated mice (14 of 17), mainly at the lower jejunal anastomosis (n = 12). The incidence of anastomotic leakage in the metamizole group was remarkably lower, occurring in four of 21 mice (19%) during the same period with three cases at the lower and one at the upper anastomosis. This is the first study demonstrating the detrimental effect of carprofen on jejunal anastomotic healing in a murine RYGB model. These findings underscore the critical importance of selecting appropriate analgesics for intestinal anastomosis surgery.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"182-192"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145834188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0