非洲爪蛙(Xenopus laevis)的自发性肝细胞瘤和胃瘤。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Bernat Martí-García, Simon L Priestnall, Yolanda Saavedra Torres, Alejandro Suárez-Bonnet
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引用次数: 0

摘要

一家动物研究机构的三只七岁大的非洲爪蛙(Xenopus laevis)出现体重减轻、腹腔膨胀和游泳步态异常等症状,出于福利考虑,该机构对这三只蛙实施了安乐死。对每只动物进行的全面尸检显示,有两只动物的左肝叶出现局灶性、坚硬、深褐色至绿色肿块,并伴有多灶性出血;另一只动物的胃部出现跨壁、坚硬、米黄色、多叶状肿块。此外,其中一只患有肝肿块的青蛙还患有血细胞瘤。经组织学诊断,肝肿块为肝细胞腺瘤,胃肿块为胃癌,并伴有经肠管转移。使用以下抗体对这三种肿瘤进行了免疫分型:细胞角蛋白 AE1/AE3、波形蛋白、E-粘连蛋白、P53 和 Ki67,此外,仅对肝肿瘤使用了突触素、Prox-1、S100 和 Sox-9。对部分病例还进行了马森三色染色、周期酸希夫染色和革兰氏染色。其中一个肝细胞腺瘤的 E-cadherin 标记为弱阳性,而另一个则只显示出不同程度的 Sox-9 阳性。基质和窦状内皮细胞上有波形蛋白标记。有趣的是,在肝脏中,细胞角蛋白 AE1/AE3 的标记仅限于胆道上皮和窦状内皮。胃癌的细胞角蛋白 AE1/AE3 仅呈阳性。本报告旨在指导实验动物兽医准确诊断两栖动物的多器官肿块。据作者所知,这是首次对X.laevis肝癌和胃癌病例系列进行全面的形态学研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Spontaneous hepatocellular and gastric tumours in African clawed frogs (Xenopus laevis).

Three seven-year-old African clawed frogs (Xenopus laevis) from an animal research facility showed weight loss, coelomic distention and an abnormal swimming gait were euthanised on welfare grounds. A complete necropsy of each animal showed a focal, firm, dark brown to green mass with multifocal haemorrhages in the left liver lobe in two animals and a transmural, firm, beige, multilobulated gastric mass in another animal. Additionally, one of the frogs with the hepatic mass had haemocoeloma. Histologically, the hepatic masses were diagnosed as hepatocellular adenomas and the gastric mass as a gastric carcinoma with trans-coelomic metastases. The three tumours were immunophenotyped using the following antibodies: cytokeratin AE1/AE3, vimentin, E-cadherin, P53 and Ki67 and, additionally, for the hepatic tumours only, synaptophysin, Prox-1, S100 and Sox-9. Masson's trichrome, Periodic acid Schiff and Gram stains was also performed in selected cases. One of the hepatocellular adenomas was weakly positively labelled with E-cadherin whereas the other showed variable positivity for Sox-9 only. Vimentin labelled the stroma and sinusoidal endothelia. Interestingly in the liver, the cytokeratin AE1/AE3 labelling was restricted to the biliary epithelium and sinusoidal endothelia. The gastric carcinoma labelled positively with cytokeratin AE1/AE3 only. This report aims to guide laboratory animal veterinarians to accurately diagnose multi-organ masses in amphibians. To the authors' knowledge, this is the first comprehensive morphological study on a case series of hepatocellular and gastric neoplasia in X. laevis.

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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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