Comparison of inferior vena cava puncture under continuous cardiac perfusion with cardiac puncture in blood acquisition of the laboratory mouse.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Wengang Hu, Hao Sheng, JiaCai Yang, Cheng Chen, Ruoyu Shang, Zhihui Liu, Xiaohong Hu, Xiaorong Zhang, Weifeng He, Chibing Huang, Gaoxing Luo
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Abstract

Obtaining sufficient blood volume from mice significantly facilitates experimental research. This study explored the inferior vena cava puncture under continuous cardiac perfusion (IVCP-UCCP) technique and evaluated its efficiency in comparison with conventional cardiac puncture (CP). In an initial dose-finding study, 50 mice were randomly assigned to one of 10 groups with escalating perfusion volume from 0.5 to 4.5 ml in 0.5-ml increments. The minimum perfusion volume was determined to be 2 ml in collecting whole circulating blood. In the next comparison using the conventional method, 40 mice were randomly assigned to one of two groups denoting different blood collection methods: Group 1: CP, Group 2: IVCP-UCCP. The results showed 1) that the cells and undiluted blood volume collected via IVCP-UCCP was over twofold higher than that by CP (p < 0.001), confirmed by the cell counts and hematoxylin-eosin staining of different tissues slides (p < 0.001); 2) the new technique did not alter the cellular composition or viability, which was verified by routine blood tests and flow cytometry (p > 0.05); 3) the blood collected via the novel technique was diluted 2.1 times: the hemato-biochemical indicator results multiplied by 2.1 were identical with the test results of blood from CP (p > 0.05). Together, the refined blood collection method of IVCP-UCCP completely extracted the limited blood resources in mice, significantly enhanced the utilization of each mouse, and thus offered scientific and ethical benefits. This technique may be also applicable for other small animal models.

比较在持续心脏灌注下进行下腔静脉穿刺和在实验室小鼠血液采集中进行心脏穿刺。
从小鼠体内获得足够的血容量极大地促进了实验研究。本研究探索了持续心脏灌注下的下腔静脉穿刺(IVCP-UCCP)技术,并评估了其与传统心脏穿刺(CP)的效率比较。在最初的剂量测定研究中,50 只小鼠被随机分配到 10 组中的一组,灌注量以 0.5 毫升为单位从 0.5 毫升递增到 4.5 毫升。在收集全循环血液时,确定最小灌注量为 2 毫升。在使用传统方法进行的下一步比较中,40 只小鼠被随机分配到两组中的一组,这两组表示不同的采血方法:第 1 组:CP,第 2 组:IVCP-UCCP。结果显示:1)通过 IVCP-UCCP 采集的细胞和未稀释血量比 CP 高出两倍多(P 0.001),不同组织切片的细胞计数和苏木精-伊红染色证实了这一点(P 0.001);2)新技术没有改变细胞的组成和活力,这一点通过血常规检测和流式细胞术得到了验证(P > 0.05);3)通过新技术采集的血液稀释了 2.1 倍:血液生化指标结果乘以 2.1 后与来自 CP 的血液检测结果相同(P > 0.05)。总之,IVCP-UCCP 的改进采血方法完全提取了小鼠有限的血液资源,大大提高了每只小鼠的利用率,从而带来了科学和伦理方面的益处。这项技术也可用于其他小动物模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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