Jianhua Huang, Yan Wang, Yan Gao, Angela Tornblom, Zerek Bianchi, Alexis Garcia, Katina Cahill, Gina Savastano
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引用次数: 0
Abstract
Accurate and humane collection of mouse urine samples is crucial for research studies and health monitoring of laboratory mouse colonies. Conventional methods may stress animals and compromise sample quality. To address these challenges, we developed a natural and animal-friendly approach using a specially designed urine collection device. This innovative technique involved individual transparent compartments with 96-well collection plates, allowing C57BL/6NTac mice to urinate freely for up to 2 h. Our study found that the optimal collection period was from 7 AM to 9 AM, during which the mice produced urine quantities ranging from 80 μl to 810 μl, with a substantial majority (85%) producing over 150 μl. The use of 96-well plates minimized stress, sample evaporation and contamination from fecal material. This cage-based non-invasive technique provides a user-friendly solution for obtaining accurate and high-quality mouse urine samples, benefiting animal welfare and facilitating rodent health surveillance and research studies.
期刊介绍：
The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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