Laboratory Animals最新文献_第8页

Comparison of two bedding types on the breeding fecundity of C57BL/6J mice. 两种垫料对 C57BL/6J 小鼠繁殖力的影响比较

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI: 10.1177/00236772241249783

Jessica L Pinckard, Lauren A Gibson, Wendy R Williams

{"title":"Comparison of two bedding types on the breeding fecundity of C57BL/6J mice.","authors":"Jessica L Pinckard, Lauren A Gibson, Wendy R Williams","doi":"10.1177/00236772241249783","DOIUrl":"10.1177/00236772241249783","url":null,"abstract":"One of the favored options for generating complex transgenic laboratory mice is through in-house breeding and management strategies. One consideration in the management of these colonies is how the animals' environment may affect reproductive success. Several aspects of the microenvironment can be controlled or manipulated, including cage type, bedding, enrichment, diet, and temperature and humidity. This study sought to evaluate reproductive outcomes for C57BL/6J mice that were randomly assigned to one of two different bedding types: paper based or corncob bedding. Our hypothesis was there would be no significant difference in reproductive outcomes between the two bedding types. A total of 10 males and 10 females were paired at 45 days of age. Animals were allowed to breed for 15 consecutive weeks. Cages were checked daily for the presence of pups and a pup count was performed at 7 days of age. Weaning occurred at 20 or 21 days of age, at which time a final pup count, pup weight, and sex were recorded. All litters born and pups weaned in the 15-week timeframe were used for data analysis. Statistical analysis compared cannibalization between the two groups and the results showed no statistical difference between groups (p > 0.05). Other parameters analyzed included average litter size, average weaning weight, and number of litters per group. All pups counted at Day 7 survived to weaning age in both groups. We concluded that both bedding types produced similar success regarding breeding fecundity in C57BL/6J mice.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"545-551"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The implementation of tunnel handling in a mouse breeding facility revealed strain-specific behavioural responses. 在小鼠育种设施中实施隧道处理后，发现了特定品系的行为反应。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2023-12-23 DOI: 10.1177/00236772231215077

Katharina Hohlbaum, Roswitha Merle, Ramona Warnke, Stefan Nagel-Riedasch, Christa Thöne-Reineke, Kristina Ullmann

{"title":"The implementation of tunnel handling in a mouse breeding facility revealed strain-specific behavioural responses.","authors":"Katharina Hohlbaum, Roswitha Merle, Ramona Warnke, Stefan Nagel-Riedasch, Christa Thöne-Reineke, Kristina Ullmann","doi":"10.1177/00236772231215077","DOIUrl":"10.1177/00236772231215077","url":null,"abstract":"As a step towards implementing non-aversive handling techniques at a big mouse breeding facility in Germany, tunnel handling was introduced in a breeding unit comprising three inbred mouse strains. To assess whether tunnel handling would be feasible for the animal technicians in their everyday work and beneficial for the mice when being handled during weekly cage change only, the behaviour of tunnel- and tail-handled animals of both sexes was examined before, during and after the handling events over a period of nine weeks. Moreover, the time expenditure was compared between both handling techniques. It was possible to use the tunnel in all three mouse strains. However, the impact of the handling techniques on the behavioural parameters investigated in the present study were strain-specific. All behavioural parameters suggested that NZW mice benefited the most from tunnel handling. The results obtained from Hello Kitty and WNK mice were ambiguous, which may suggest that a brief handling session during the cage clean may have not been sufficient to habituate them to the process of handling. It took the animal technicians approximately 3 seconds longer per mouse when using a tunnel. The strain-specific results should encourage researchers to share their experiences with non-aversive handling techniques in different mouse strains, for example, along with their research articles.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"552-564"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spontaneous type II cell-derived bronchioloalveolar adenoma in a young ICR mouse. 一只幼年 ICR 小鼠自发性 II 型细胞衍生支气管肺泡腺瘤。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1177/00236772241257726

Minkyoung Sung, Soo-Eun Sung, Joo-Hee Choi, Yujeong Lee, Kyung-Ku Kang, SunHee Park, Duyeol Kim, Sijoon Lee

引用次数: 0

Refining urine collection in mice: Development of an innovative urine collection device. 改进小鼠的尿液收集：开发创新型尿液收集装置

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI: 10.1177/00236772231219828

Mélodie Douté, Céline Monzali, Antonino Nicoletti, Giuseppina Caligiuri, Marc Clement

引用次数: 0

How cage effects can hurt statistical analyses of completely randomized designs. 笼效应如何影响完全随机设计的统计分析？

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-09-24 DOI: 10.1177/00236772241276785

Reid D Landes

引用次数: 0

Methods for applying blinding and randomisation in animal experiments. 在动物实验中应用盲法和随机化的方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 DOI: 10.1177/00236772241272991

P S Verhave, R van Eenige, Iacw Tiebosch

引用次数: 0

Study design: think 'scientific value' not 'p-values'. 研究设计：要考虑 "科学价值"，而不是 "P 值"。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 DOI: 10.1177/00236772241276806

Penny S Reynolds

引用次数: 0

Depicting variability and uncertainty using intervals and error bars. 使用区间和误差条描述可变性和不确定性。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-09-05 DOI: 10.1177/00236772241247105

Naomi Altman, Martin Krzywinski

引用次数: 0

About statistical significance, and the lack thereof. 关于统计意义，以及缺乏统计意义的问题。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-08-19 DOI: 10.1177/00236772241248509

Fulvio Magara, Benjamin Boury-Jamot

{"title":"About statistical significance, and the lack thereof.","authors":"Fulvio Magara, Benjamin Boury-Jamot","doi":"10.1177/00236772241248509","DOIUrl":"10.1177/00236772241248509","url":null,"abstract":"Absence of statistical significance (i.e., p > 0.05) in the results of a frequentist test comparing two samples is often used as evidence of absence of difference, or absence of effect of a treatment, on the measured variable. Such conclusions are often wrong because absence of significance may merely result from a sample size that is too small to reveal an effect. To conclude that there is no meaningful effect of a treatment/condition, it is necessary to use an appropriate statistical approach. For frequentist statistics, a simple tool for this goal is the 'two one-sided t-test,' a form of equivalence test that relies on the a priori definition of a minimal difference considered to be relevant. In other words, the smallest effect size of interest should be established in advance. We present the principles of this test and give examples where it allows correct interpretation of the results of a classical t-test assuming absence of difference. Equivalence tests are also very useful in probing whether certain significant results are also biologically meaningful, because when comparing large samples it is possible to find significant results in both an equivalence test and in a two-sample t-test, assuming no difference as the null hypothesis.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"448-452"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testing for normality: a user's (cautionary) guide. 正态性测试：用户（警示）指南。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-10-01 DOI: 10.1177/00236772241276808

Romain-Daniel Gosselin

{"title":"Testing for normality: a user's (cautionary) guide.","authors":"Romain-Daniel Gosselin","doi":"10.1177/00236772241276808","DOIUrl":"https://doi.org/10.1177/00236772241276808","url":null,"abstract":"The normality assumption postulates that empirical data derives from a normal (Gaussian) population. It is a pillar of inferential statistics that enables the theorization of probability functions and the computation of p-values thereof. The breach of this assumption may not impose a formal mathematical constraint on the computation of inferential outputs (e.g., p-values) but may make them inoperable and possibly lead to unethical waste of laboratory animals. Various methods, including statistical tests and qualitative visual examination, can reveal incompatibility with normality and the choice of a procedure should not be trivialized. The following minireview will provide a brief overview of diagrammatical methods and statistical tests commonly employed to evaluate congruence with normality. Special attention will be given to the potential pitfalls associated with their application. Normality is an unachievable ideal that practically never accurately describes natural variables, and detrimental consequences of non-normality may be safeguarded by using large samples. Therefore, the very concept of preliminary normality testing is also, arguably provocatively, questioned.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":"58 5","pages":"433-437"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0