Laboratory Animals最新文献_第8页

Retraction: "Advances in research on the biology of the desert rodent Gerbillus tarabuli: a review". 撤回：“沙漠啮齿动物沙鼠塔拉布利生物学研究进展：综述”。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-12-01 Epub Date: 2025-11-18 DOI: 10.1177/00236772251382865

引用次数: 0

Antinociceptive and motor-blocking effects of epidurally administered diphenhydramine at different concentrations in conscious rabbits: a randomized-controlled trial. 硬膜外注射不同浓度苯海拉明对清醒家兔的抗痛觉和运动阻断作用：一项随机对照试验。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-12-01 Epub Date: 2025-08-28 DOI: 10.1177/00236772251351102

Navid Ziaei-Darounkolaei, Ismael Hernández-Ávalos, Vahid Vahedian, Majid Soleimannejad, Alireza Kaveh-Aski, Hossein Rezaei, Mohsen Keyhani, Bahador Barari

{"title":"Antinociceptive and motor-blocking effects of epidurally administered diphenhydramine at different concentrations in conscious rabbits: a randomized-controlled trial.","authors":"Navid Ziaei-Darounkolaei, Ismael Hernández-Ávalos, Vahid Vahedian, Majid Soleimannejad, Alireza Kaveh-Aski, Hossein Rezaei, Mohsen Keyhani, Bahador Barari","doi":"10.1177/00236772251351102","DOIUrl":"10.1177/00236772251351102","url":null,"abstract":"In this study we aimed to determine the optimal epidural dose and concentration of diphenhydramine (DPH) for prolonged analgesia while preserving motor function, comparing its effects with lidocaine. A single-blind, randomized-controlled trial was conducted with 32 healthy rabbits assigned to two experiments (2.5 ± 0.5 kg). Experiment 1 (n = 25) assessed optimal DPH doses (4, 8, 12, 18, 24 mg kg-1), while Experiment 2 (n = 15) compared DPH concentrations (5%, 10%, 15%, 20%) at 12 mg kg-1. Antinociceptive effects were measured using pinprick tests on the perineal and digital areas, and motor blockade was evaluated using a modified 4-point grading system for limb paralysis. The results showed that epidural administration of 10% DPH at 12 mg kg-1 provided profound analgesia, with hindlimb analgesia lasting 201 ± 2.5 min and motor blockade persisting for 157.2 ± 3.4 min. In contrast, 5% DPH resulted in 96 ± 15 min of analgesia and 5.2 ± 2.2 min of motor block. Lidocaine failed to provide effective digital analgesia. No significant difference was observed between 10% and 15% DPH in analgesic and motor effects. The motor blockade duration was consistently shorter than the antinociceptive effect. DPH demonstrated superior potency, and a prolonged, sensory-selective epidural block compared with lidocaine, minimizing motor blockade and the risk of akinesia-like analgesia. DPH (preferably >5%) offered effective and safe analgesia, making it a promising option for hindlimb analgesia in rabbits, reducing animal suffering and providing better outcomes than lidocaine.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"676-691"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The (re)turn of the 3Rs: an inquiry into the normative nature of Russell and Burch's principles of humane experimental technique - their misunderstanding, reform and implementation through an ethics tool. 3r的（再）转向：探究罗素和伯奇的人道实验技术原则的规范性——他们的误解、改革和通过伦理工具的实施。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-10-01 Epub Date: 2025-06-12 DOI: 10.1177/00236772251326352

Herwig Grimm, Marc Dusseldorp

{"title":"The (re)turn of the 3Rs: an inquiry into the normative nature of Russell and Burch's principles of humane experimental technique - their misunderstanding, reform and implementation through an ethics tool.","authors":"Herwig Grimm, Marc Dusseldorp","doi":"10.1177/00236772251326352","DOIUrl":"10.1177/00236772251326352","url":null,"abstract":"The 3Rs strongly shape the practice of laboratory animal use, as well as related policies worldwide. This success should not obscure the fact that implementing the 3Rs comes with challenges. A major problem is that it is fundamentally unclear under which conditions the 3Rs may be considered fulfilled in specific contexts. We argue that this lack of clarity is largely a result of the fact that the normative nature of the 3Rs has so far been disregarded. Hence, this paper seeks to answer the following research question: how is the normative nature of the 3Rs to be understood, and how can this understanding transparently guide their implementation? Based on a distinction between different types of norms, we show that the 3Rs, which have been called 'principles' since their origin, are indeed to be understood as principles in a substantive (norm-theoretical) sense. That is, they are norms that command the highest possible realization of their content. This understanding of the normative nature of the 3Rs has a significant effect on their implementation in practical contexts. As we will argue, it turns the orthodox idea of implementation strategies upside down. Building on this theoretical claim, we propose an ethics tool designed to help applicants, review committee members and authorities to apply the 3Rs transparently and, above all, in accordance with a reflected understanding of the relevant EU Directive's intention (Directive 2010/63/EU) and of the work of Russell and Burch, the pioneers of this milestone in the promotion of animal welfare in research.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"556-569"},"PeriodicalIF":1.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12583627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Teaching experimental design: outputs from the FELASA Working Group. 教学实验设计：FELASA工作组的产出。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-10-01 Epub Date: 2025-06-09 DOI: 10.1177/00236772241295308

Derek Fry, Manuel Berdoy, Monica Forni, Carlos Oscar S Sorzano, Thomas Steckler, Nuno H Franco

{"title":"Teaching experimental design: outputs from the FELASA Working Group.","authors":"Derek Fry, Manuel Berdoy, Monica Forni, Carlos Oscar S Sorzano, Thomas Steckler, Nuno H Franco","doi":"10.1177/00236772241295308","DOIUrl":"10.1177/00236772241295308","url":null,"abstract":"Good education and training for scientists undertaking animal experiments is important for providing understanding of key issues in experimental design (ED) and for alleviating continuing concerns about the conduct of animal and in vitro research studies. We present here outputs from the FELASA Experimental Design Working Group, set up to consider the current provision of ED teaching and how it might be improved and harmonised across the laboratory animal science community. It is hoped that these outputs will provide practical help to ED teachers who wish to enhance the effectiveness of their teaching; they include• A list of learning outcomes (LOs) that should be achieved by learners, principally aimed at early career researchers;• An example of an (adaptable) template of how these LOs could be addressed in 16 h (12 h tuition plus breaks), ideally as a 2-day workshop. If circumstances make 12 h tuition impossible to achieve, key LOs for a shorter course are identified;• Guidance and recommendations for running ED courses, including some ideas for achieving effective learning, the ideal skill set for tutors, some teaching scenarios, and the amount of statistics to have in a basic experimental design course;• A glossary of relevant terms (in supplemental material);• A description of how the 2-day course format ran on two trial occasions, with results of informal assessment of participants as well as their feedback, both immediate and a year afterwards, indicating it was effective;• A programme for a potential 2-day, training-the-trainers style, workshop, describing its key elements and the results of trialling this with a range of ED tutors.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"614-629"},"PeriodicalIF":1.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12583620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating cervical dislocation methods, without using tension on the tail, for humanely killing adult laboratory mice. 评价在不使用尾部张力的情况下，颈椎脱臼法对成年实验小鼠的人道杀伤效果。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-10-01 Epub Date: 2025-06-09 DOI: 10.1177/00236772251332722

Antony Davidge, Flaviu Bulat, Aude Vernet

{"title":"Evaluating cervical dislocation methods, without using tension on the tail, for humanely killing adult laboratory mice.","authors":"Antony Davidge, Flaviu Bulat, Aude Vernet","doi":"10.1177/00236772251332722","DOIUrl":"10.1177/00236772251332722","url":null,"abstract":"Cervical dislocation (CD) is a widely used method worldwide for humanely killing adult laboratory mice in accordance with national legislation, such as the Animals (Scientific Procedures) Act 1986 in the UK. However, concerns have been raised regarding the limitations and potential risks associated with CD, including a reported failure rate of up to 20% and the risk of injury to the thoracic or lumbar spine region. To address these concerns, we have adopted a CD method that avoids the use of tension on the tail or any additional tools. In this study, we detail our process of validation through self-reporting and direct observation leading up to present our implementation of computerised tomography and three-dimensional imaging software to evaluate the effectiveness and efficiency of this tail-force free CD method. Our findings reveal a 100% success rate in achieving accurate cervical dislocation without causing damage to other vertebrae, thereby providing an improved and more reliable approach to humane killing for both male and female adult laboratory mice.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"570-577"},"PeriodicalIF":1.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12583621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods of humane killing of laboratory fish: FELASA Working Group recommendations. 人道杀死实验鱼的方法：FELASA工作组的建议。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-10-01 Epub Date: 2025-08-13 DOI: 10.1177/00236772251351095

Jean-Philippe Mocho, Juan Ramos Blasco, Pia Rengtved Lundegaard, Robin McKimm, Vlasta Jenčič, Kristine von Krogh

{"title":"Methods of humane killing of laboratory fish: FELASA Working Group recommendations.","authors":"Jean-Philippe Mocho, Juan Ramos Blasco, Pia Rengtved Lundegaard, Robin McKimm, Vlasta Jenčič, Kristine von Krogh","doi":"10.1177/00236772251351095","DOIUrl":"10.1177/00236772251351095","url":null,"abstract":"As commissioned by the Federation of European Laboratory Animal Science Associations, these working group recommendations define the requirements to achieve the humane killing of fish, compare methods of killing, recommend methods of killing depending on context, and detail protocols leading to good practice. With a review of current practices in a fish laboratory and available literature to guide the recommendations, the concept of ideal euthanasia is discussed, and the dilemma of a prompt but stressful death versus a slow but stress-free experience is introduced. Noticeably, the context of fish killing varies widely whether to satisfy European Directive requirements, efficacy for species and/or developmental stages, scientific needs, health and safety, or animal welfare. Examples in the recommendations are based on the most common laboratory fish species, such as zebrafish Danio rerio, and the most commonly used methods of killing, such as overdose of anaesthesia, hypothermic shock, electrical stunning, and concussion - percussive blow to the head. Practical applications of completion of death, refinements, and protocols for good practice are proposed for all developmental stages and depending on the potential fate of the carcass as a scientific sample.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"599-613"},"PeriodicalIF":1.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12583636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A scoping review on reporting of methods in DSS colitis mouse models. DSS结肠炎小鼠模型研究方法综述。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-10-01 Epub Date: 2025-06-14 DOI: 10.1177/00236772251331677

Michaela Thallmair, Paulin Jirkof

{"title":"A scoping review on reporting of methods in DSS colitis mouse models.","authors":"Michaela Thallmair, Paulin Jirkof","doi":"10.1177/00236772251331677","DOIUrl":"10.1177/00236772251331677","url":null,"abstract":"Inflammatory bowel diseases (IBDs) are a major health burden, and incidence as well as prevalence have increased over the last decades. Colitis animal models are used to explore the underlying pathogenesis of and therapeutic options for IBD. Since the reporting of specific aspects of colitis studies using mice has been criticized in the past, we performed a scoping review based on the PRISMA guidelines to assess putative improvements in the quality of reporting. A defined search for dextran sulfate sodium (DSS) murine colitis models was performed in three literature databases (PubMed, PubMed Central®, and Embase) for two time periods: 2007/2008 and 2017. Data were extracted from 122 articles published in 2007/2008 and 236 publications from 2017. We checked the articles for the reporting of details of the colitis model (DSS properties, manufacturer, concentration, duration of application, mouse strain, sex, source), measures to reduce allocation, performance and detection bias (randomization and blinding), and information on clinical assessment, refinements, and humane endpoints. Our results showed that there was significant improvement over time in the scores for refinement and, based on this, also the completeness score. However, the other aspects were poorly reported, suggesting that this research field may need to adopt reporting guidelines such as ARRIVE, the Gold Standard Publication Checklist, or the colitis methods checklist when writing and reviewing publications.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"541-555"},"PeriodicalIF":1.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex and strain differences in the micropipette-guided drug administration (MDA) method in mice. 小鼠微管引导给药（MDA）方法的性别和品系差异。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-10-01 Epub Date: 2025-06-03 DOI: 10.1177/00236772251320843

Sarah Steiner, Olga Krzyzaniak, Frida A M Nilsson, Macsmeila Dietrich, Laura Kämpfen, Pål Johansen, Paulin Jirkof, Urs Meyer

{"title":"Sex and strain differences in the micropipette-guided drug administration (MDA) method in mice.","authors":"Sarah Steiner, Olga Krzyzaniak, Frida A M Nilsson, Macsmeila Dietrich, Laura Kämpfen, Pål Johansen, Paulin Jirkof, Urs Meyer","doi":"10.1177/00236772251320843","DOIUrl":"10.1177/00236772251320843","url":null,"abstract":"To provide an alternative to oral gavage for per os treatments in laboratory mice, we have recently developed and introduced the micropipette-guided drug administration (MDA) method. This procedure is based on the presentation of a palatable solution consisting of sweetened condensed milk diluted with water, encouraging voluntary consumption of the vehicle and drug formulations. In this study, we compared the MDA method in male and female C57BL/6N and BALB/c mice, two inbred strains widely used in basic and preclinical research. Administering a diluted condensed milk solution daily over a period of 30 days, we observed that male C57BL/6N mice reached the fully voluntary drinking stage within five days or less, requiring the least amount of time to drink from the micropipette. Compared with males, female C57BL/6N mice showed increased consumption times during the initial administration period, yet they all managed to reach a fully voluntary stage within seven days or less. By contrast, BALB/c mice in general, and female BALB/c mice in particular, consistently required more time to consume the diluted condensed milk solution, whether administered voluntarily (no restraint) or semi-voluntarily (with mild tail restraint). Notably, a substantial portion of BALB/c mice (12.5% of males and 62.5% of females) failed to achieve fully voluntary consumption by MDA, despite their ability and willingness to drink the condensed milk solution when using a mild tail restraint. Taken together, the present study identified significant strain and sex differences in the MDA method as applied to laboratory mice.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"578-587"},"PeriodicalIF":1.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12583613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new method to replace oral gavage for the study of Cryptosporidium infection in mice. 替代灌胃研究小鼠隐孢子虫感染的新方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-10-01 Epub Date: 2025-04-11 DOI: 10.1177/00236772251318406

Fernanda G Fumuso, Christine E Salomon, Roberta M O'Connor

{"title":"A new method to replace oral gavage for the study of Cryptosporidium infection in mice.","authors":"Fernanda G Fumuso, Christine E Salomon, Roberta M O'Connor","doi":"10.1177/00236772251318406","DOIUrl":"10.1177/00236772251318406","url":null,"abstract":"Oral gavage is a widely used method to infect mice with Cryptosporidium parvum (CP), the most common animal model of infection. Cryptosporidium spp. are worldwide distributed, gastrointestinal parasites that mainly cause diarrhea in humans and neonatal ruminants. CP is transmitted via the fecal-oral route, usually through contaminated water. In rodent models of Cryptosporidium infection, oral gavage is used as the route of infection. This method, while effective, induces a generalized stress response and requires specific skills and experience. Our aim was to replace oral gavage by a refined method using a mixture palatable to mice, where oocysts could be mixed in. A peanut butter gelatin mix (PBG) containing CP oocysts was developed and used for voluntary oral infection of mice. We were able to confirm CP infections in young interferon-gamma knock out mice by detecting oocyst shedding in feces, demonstrating that the PBG method successfully produced infections similar to those obtain through oral gavage, and could be used for other pathogens or as a method to deliver experimental therapeutics or other substances.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"593-598"},"PeriodicalIF":1.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12354134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A customised combination of environmental enrichment reduces aggression in CD-1 male mice. 定制的环境富集组合减少了CD-1雄性小鼠的攻击性。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-10-01 Epub Date: 2025-07-21 DOI: 10.1177/00236772251333986

Amy Veness, Christophe Galichet, Sian Murphy, Tina O'Mahony, Yoh Isogai, Eleni M Amaniti

引用次数: 0