Mélodie Douté, Céline Monzali, Antonino Nicoletti, Giuseppina Caligiuri, Marc Clement
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引用次数: 0
Abstract
Urine collection can be challenging in studies involving small rodents like mice, as the actual methods of collection are anxiogenic and constrain animal welfare while having high variability in the volume of urine collected. To improve the current methods and eventually reduce the impact on the well-being of mice, we developed an innovative 3D-printed urine collection device (UCD). This two-compartment UCD is shaped to fit in classical husbandry cages and allows urine collection by spontaneous urination from two mice housed in their own cage without cross-contamination while enabling potential social interactions. We used our UCD to study the evolution of urinary parameters related to renal functions in a model of antibody-mediated chronic kidney disease. Overall, we report here a time-saving and affordable method for urine collection providing a large amount of uncontaminated urine and which we believe may improve animal welfare in comparison with other methods.
在涉及小鼠等小型啮齿类动物的研究中，尿液收集是一项具有挑战性的工作，因为实际的尿液收集方法会使小鼠产生焦虑情绪，限制动物福利，同时收集的尿液量变化很大。为了改进现有方法并最终减少对小鼠福利的影响，我们开发了一种创新的 3D 打印尿液收集装置（UCD）。这种双室尿液收集装置的形状适合传统的饲养笼，可通过饲养在各自笼子里的两只小鼠的自发排尿收集尿液，不会造成交叉感染，同时还能实现潜在的社交互动。我们利用 UCD 研究了抗体介导的慢性肾病模型中与肾功能相关的尿液参数的演变。总之，我们在此报告了一种既省时、又经济实惠的尿液收集方法，它能提供大量未受污染的尿液，与其他方法相比，我们认为这种方法可以改善动物福利。
期刊介绍：
The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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