Laboratory Animals最新文献_第6页

LASA 60th Annual Meeting - Championing Public Engagement with "Snippets of the 3Rs". 第 60 届 LASA 年会--"3R "片段倡导公众参与。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 DOI: 10.1177/00236772241232911

Sophie Kimpton, Jamie Redden

引用次数: 0

Software for animal randomization: A tool for increasing the reproducibility of science. 动物随机化软件：增加科学再现性的工具。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 Epub Date: 2023-10-28 DOI: 10.1177/00236772231194957

Liana Monteiro da Fonseca Cardoso, Natiele Carla da Silva Ferreira, Matheus de Araripe Lopes Correa, Sérgio Alves da Silva, Luiz Anastacio Alves

{"title":"Software for animal randomization: A tool for increasing the reproducibility of science.","authors":"Liana Monteiro da Fonseca Cardoso, Natiele Carla da Silva Ferreira, Matheus de Araripe Lopes Correa, Sérgio Alves da Silva, Luiz Anastacio Alves","doi":"10.1177/00236772231194957","DOIUrl":"10.1177/00236772231194957","url":null,"abstract":"Poorly designed preclinical studies may compromise human health due to erroneous conclusions regarding treatment effects in addition to contributing to experimental irreproducibility and wasted resources. Randomization is one of the crucial steps to enhance scientific rigor and is a commonly recognized bias-reducing instrument that increases the reliability and reproduction of studies involving animals (even with syngeneic animals). This procedure should be considered when planning a study and reported during data publication. In this context, this work aimed to highlight the importance of adopting quality measures in preclinical trials, with an emphasis on animal randomization. The 'Mouse Randomization' app was developed to help researchers estimate an adequate sample size to obtain significant statistical power, ensuring the ethical use of animals. This app is freely available on the internet to carry out animal randomization and calculate sample sizes for in vivo experiments. We believe that this brief discussion about animal randomization could raise awareness among researchers on how to improve the quality of preclinical research, increasing reproducibility and avoiding animal misuse.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66784018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Twelve years after the ARRIVE guidelines: Animal research has not yet arrived at high standards. ARRIVE指南发布12年后：动物研究尚未达到高标准。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 Epub Date: 2023-09-20 DOI: 10.1177/00236772231181658

Junmin Song, Marco Solmi, Andre F Carvalho, Jae Il Shin, John Pa Ioannidis

{"title":"Twelve years after the ARRIVE guidelines: Animal research has not yet arrived at high standards.","authors":"Junmin Song, Marco Solmi, Andre F Carvalho, Jae Il Shin, John Pa Ioannidis","doi":"10.1177/00236772231181658","DOIUrl":"10.1177/00236772231181658","url":null,"abstract":"The reproducibility crisis across animal studies jeopardizes the credibility of the main findings derived from animal research, even though these findings are critical for informing human studies. To clarify and improve transparency among animal studies, the ARRIVE reporting guidelines were first announced in 2010 and upgraded to version 2.0 in 2020. However, compliance with and awareness of those reporting guidelines has remained suboptimal. Journal editors should encourage the authors to adhere to those guidelines. Authors, editors, referees, and reviewers should be aware of the ARRIVE guideline 2.0 when assessing and evaluating the methodology and findings of animal studies. However, we should also question whether reporting guidelines alone can change a research culture and improve the reproducibility of animal investigations. Reported research may not reflect actual research. Large segments of animal research efforts are wasted because of poor design choices and because of non-publication rather than suboptimal reporting. Better training of the scientific workforce, interventions at improving animal research at the design stage, registration practices, and alignment of the reward system with the publication of rigorous animal research may achieve more than reporting guidelines alone.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41117029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing United States and European Union academic animal programs: Finances. 比较美国和欧盟学术动物项目：资金。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 Epub Date: 2023-09-20 DOI: 10.1177/00236772231152749

Patricia Preisig, James D Macy, Jann Hau

{"title":"Comparing United States and European Union academic animal programs: Finances.","authors":"Patricia Preisig, James D Macy, Jann Hau","doi":"10.1177/00236772231152749","DOIUrl":"10.1177/00236772231152749","url":null,"abstract":"The Yale Animal Resource Cost and Benchmarking survey, conducted in United States (US) academic animal research/resource centres (ARC), was modified to capture similar information in European Union (EU) (including the non-EU countries Switzerland and the United Kingdom) academic ARCs, who are members of the League of European Research Universities (LERU). Participating institutions came from Denmark, England, Finland, France, Germany, Ireland, Italy, Netherlands, Scotland, Spain and Switzerland. Survey data analysis suggests that (a) per diem rates have similar compositions in LERU and US programs, with >50% of the rates dedicated to recovering salary and fringe, followed by supplies (∼25%), facility costs (∼10%) and other expenses (∼15%); (b) ∼60% of US and LERU programs under-recover mouse care costs; (c) on average, LERU programs have a small positive net-operating balance, while US programs average a large deficit; (d) in LERU programs <50% of institutions fund the animal program deficit, while in US programs almost 100% of such deficits are covered by the institution; and (e) when setting per diem rates, both US and LERU programs rank cost accounting as the most influential factor. Both US and LERU programs are reluctant to raise per diem rates to the extent required to recover costs and, thus, continue to under-recover costs, resulting in the animal program being 'caught in the middle' between the competing financial challenges of investigator 'affordability' and the animal program's fiduciary responsibility to the institution.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41135238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Words from the FELASA President. FELASA 主席的话

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 DOI: 10.1177/00236772241242736

Klas Abelson

引用次数: 0

Validation of computed tomography as a diagnostic tool in guinea pigs with non-alcoholic fatty liver disease. 计算机断层扫描作为非酒精性脂肪性肝病豚鼠诊断工具的验证

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 Epub Date: 2023-11-24 DOI: 10.1177/00236772231182511

Kristine Lintrup, David Højland Ipsen, Josephine Skat-Rørdam, Jens Lykkesfeldt, Pernille Tveden-Nyborg, Lene Elisabeth Buelund

{"title":"Validation of computed tomography as a diagnostic tool in guinea pigs with non-alcoholic fatty liver disease.","authors":"Kristine Lintrup, David Højland Ipsen, Josephine Skat-Rørdam, Jens Lykkesfeldt, Pernille Tveden-Nyborg, Lene Elisabeth Buelund","doi":"10.1177/00236772231182511","DOIUrl":"10.1177/00236772231182511","url":null,"abstract":"Non-alcoholic fatty liver disease (NAFLD) and subsequent steatohepatitis (NASH) is the most common cause of liver disease and liver transplantation in humans. Affecting millions of patients worldwide, diagnosis relies on a biopsy, not without risk to the patient, and emphasises the need for improved diagnostic measures to determine and monitor disease progression. Despite intensive research, approved pharmacological treatment modalities are few, underlining that animal models with increased translational validity are important to advance preclinical drug development. This study validates the applicability of computed tomography (CT) as a non-invasive diagnostic tool for the assessment of liver steatosis in a guinea pig model of NAFLD/NASH. Guinea pigs with induced NAFLD or NASH were compared to healthy controls at two separate time points: week 16, serving as baseline measure, and week 25 to monitor disease progression over time. The animals were subsequently euthanised, and samples were collected to confirm disease stage. The data showed a strong negative correlation between liver triglycerides and Hounsfield unit (HU) values (R2 = 0.8157; p < 0.0001). A significant difference in histopathological scoring and HU values between grade 0 and more advanced stages of steatosis was recorded (p < 0.001), although the degree of liver fibrosis could not be accurately evaluated by differences in HU. In conclusion, the present study validates CT scanning for the determination of hepatic steatosis in guinea pigs, and it strongly supports the technique as a relevant non-invasive diagnostic tool in this species.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138299369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Watch out for five felasa working group recommendations. 关注 felasa 工作组的五项建议。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 DOI: 10.1177/00236772241242729

Livia D'Angelo

引用次数: 0

Eugenol as anesthetic for Cnesterodon decemmaculatus (Cyprinodontiformes, Poeciliidae). Eugenol作为去甲Cnesterodon decemmaculatus（Cyprinodonformes，Poecilidae）的麻醉剂。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-02-01 Epub Date: 2023-09-15 DOI: 10.1177/00236772231192020

Maria C Vercellini, Ramiro Rearte, Luca di Cesare, Miguel A Ayala, Martin M Montes

{"title":"Eugenol as anesthetic for Cnesterodon decemmaculatus (Cyprinodontiformes, Poeciliidae).","authors":"Maria C Vercellini, Ramiro Rearte, Luca di Cesare, Miguel A Ayala, Martin M Montes","doi":"10.1177/00236772231192020","DOIUrl":"10.1177/00236772231192020","url":null,"abstract":"Anesthetics are commonly used in fish for surgery and to facilitate capture, handling and transport in aquaculture and experimental procedures. In research, the selection of the anesthetic depends on its properties and on the recovery time. Eugenol has been pointed out as an effective anesthetic for fish, alternative to traditional drugs. Although Cnesterodon decemmaculatus is widely used as a model in ecological bioassays, no anesthetic protocol has been reported for this species. The aim of the present study was to evaluate the induction time (i.e. time to reach anesthetic stage VI) and recovery time in individuals of C. decemmaculatus subjected to eugenol at a fixed concentration, according to sex and pregnancy status. Forty-one fish were divided into three groups: males, pregnant females and non-pregnant females. They were measured for total length, standard length and weight, and the condition factor (K) was calculated. No significant differences in induction and recovery times were found for sex, pregnancy status and K between groups. Results are a contribution toward the development protocol of a standard anesthetic protocol for C. decemmaculatus.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10235418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ERK1/2 modulation in the central nervous system of male and female thiamine-deficient mice with amprolium. 安普罗林对雌雄硫胺素缺乏小鼠中枢神经系统中 ERK1/2 的调节作用

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-02-01 Epub Date: 2023-09-08 DOI: 10.1177/00236772231191586

Mirian P da Silva, Francisco Wb Lima, Adha Gs Moura, Julia P Nunes, Clarissa As de Cordova, Fabiano M de Cordova

{"title":"ERK1/2 modulation in the central nervous system of male and female thiamine-deficient mice with amprolium.","authors":"Mirian P da Silva, Francisco Wb Lima, Adha Gs Moura, Julia P Nunes, Clarissa As de Cordova, Fabiano M de Cordova","doi":"10.1177/00236772231191586","DOIUrl":"10.1177/00236772231191586","url":null,"abstract":"Thiamine deficiency experimental models focus on using the pyrithiamine analog in male rodents, making the thiamine deficiency effects in females and the use of other thiamine antagonists, such as amprolium, unknown. We investigated the impact of thiamine deficiency with amprolium in the cerebral cortex and thalamus of male and female mice by evaluating the modulation of ERK1/2 phosphorylation. The animals were exposed for 20 days to thiamine-deficient chow with different doses of amprolium (20, 40, 60 and 80 mg/kg) and at different treatment periods (five, 10, 15 or 20 days) at a dose of 60 mg/kg. After treatments, ERK1/2 phosphorylation was analyzed by western blot. In male mice, we observed a progressive increase in ERK1/2 phosphorylation in both the cerebral cortex and thalamus in response to the dose of amprolium. In females, ERK1/2 phosphorylation did not progressively increase in response to the amprolium dosage. However, an increase in phosphorylation at the higher doses of 60 and 80 mg/kg was observed. We observed a more intense increase in ERK1/2 phosphorylation in males' cerebral cortex and thalamus from 10 days onwards. In females, the ERK1/2 modulation profiles were similar. The results show that thiamine deficiency induction with amprolium is efficient, compatible with other recognized models that use pyrithiamine, showing changes in cell signaling in the nervous system. The study showed differences in response to thiamine deficiency with amprolium between male and female mice in relation to ERK1/2 phosphorylation and demonstrated that females respond positively to the method and can also be used as model animals.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10178290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

XVII SECAL CONGRESS Santiago de Compostela (Spain), 14-17 November 2023. 第十七届 SECAL 大会圣地亚哥-德孔波斯特拉（西班牙），2023 年 11 月 14-17 日。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-02-01 DOI: 10.1177/00236772231225388

引用次数: 0