Jurnal e-Biomedik最新文献

{"title":"Hubungan Nilai Ujian Try Out Lokal dengan Nilai Computer Based Test Uji Kompetensi Mahasiswa Program Profesi Dokter di Fakultas Kedokteran Universitas Sam Ratulangi Manado","authors":"Atika I. S. Labatjo, F. Manoppo, Siemona L. E. Berhimpon","doi":"10.35790/EBM.7.1.2019.23532","DOIUrl":"https://doi.org/10.35790/EBM.7.1.2019.23532","url":null,"abstract":"Abstract: The graduation rate of the Computer Based Test (CBT) in Competency Test for Medical Student Profession Program (UKMPPD) of Medical Faculty, Sam Ratulangi University is still below 50% so far. Therefore, the Medical Faculty of Sam Ratulangi University makes various efforts to increase their graduation rate, inter alia implementation of local Try Out (TO) test as a filtering test required before the CBT UKMPPD. This study was aimed to find out whether there was a correlation between the local TO test score and the CBT UKMPPD score. This was a descriptive analytical study with a cross-sectional design. Data of this study were the local TO test score and the CBT UKMPPD score of the Medical Faculty of Sam Ratulangi University. The data were analyzed with the Spearman correlation test. The results showed that there was a significant correlation (P=0.028) between the local TO test score and the CBT UKMPPD score with a very weak positive correlation value (r= 0.199). Conclusion: There was a significant correlation between the local TO test score and the CBT UKMPPD score.Keywords: TO, CBT, UKMPPD Abstrak: Angka kelulusan Computer Based Test (CBT) Ujian Kompetensi Mahasiswa Program Profesi Dokter (UKMPPD) Fakultas Kedokteran Universitas Sam Ratulangi (FK Unsrat) Manado masih berada di bawah 50%. Pihak FK Unsrat melakukan berbagai upaya agar angka kelulusan UKMPPD FK Unsrat meningkat, salah satunya yaitu dengan mengadakan ujian Try Out (TO) lokal sebagai ujian penyaring yang wajib diikuti sebelum CBT UKMPPD. Penelitian ini bertujuan untuk mengetahui apakah terdapat hubungan antara nilai ujian TO lokal dengan nilai CBT UKMPPD. Jenis penelitian ialah deskriptif analitik dengan ddesain potong lintang. Data penelitian ialah nilai ujian TO lokal dan CBT UKMPPD FK Unsrat. Data dianalisis menggunakan uji korelasi Spearman. Hasil penelitian mendapatkan hubungan bermakna (P=0,028) antara nilai ujian TO lokal dengan nilai CBT dengan tingkat keeratan positif yang sangat lemah (r=0,199). Simpulan: Terdapat hubungan bermakna antara nilai ujian TO lokal dengan nilai CBT UKMPPD.Kata kunci: TO, CBT, UKMPPD","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78564530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gambaran Protein Urin pada Primigravida Trimester I dengan Riwayat Orang Tua Hipertensi di Kota Manado","authors":"Ni L. A. Sucindrawati, Glady I. Rambert, Siemona L. E. Berhimpon","doi":"10.35790/EBM.7.1.2019.23531","DOIUrl":"https://doi.org/10.35790/EBM.7.1.2019.23531","url":null,"abstract":"Abstract: Proteinuria is an excessive excretion of protein in urine. It is defined as 300 mg or more potein within 24 hours or 30 mg/Dl (+1 dipstick ) permanently in random urine samples. The main cause of proteinuria in pregnant woman is any desease associated with damage of the urinary tract organs. Furthermore, other causes of proteinuria in pregnant woman are pre-eclampsia and eclampsia, influenced by parental history of hypertension. This study was aimed to obtain the protein level in first trimester of primigravidas with parental history of hypertension in Manado. This was an observational descriptive study with a cross sectional design. Subjects were taken by using non-probality sampling consecutive sampling type to obtain urine samples from all subjects that matched the criteria and the specified time. The results showed that of 15 urine samples, there were 9 samples of positive protein (60%). Conclusion: Protein was found in urine samples of some primigravidas with parental history of hypertension in Manado.Keywords: proteinuria, pre-eclampsia, parental history of hypertension Abstrak: Proteinuria adalah ekskresi protein berlebihan dalam urin. Proteinuria didefinisikan sebagai terdapatnya 300 mg atau lebih protein dalam 24 jam atau 30mg/dL (1+ dipstik) secara menetap pada sampel acak urin. Penyebab utama terjadinya proteinuria pada ibu hamil ialah penyakit yang berhubungan dengan kerusakan pada organ sistem traktus urinarius. Penyebab lain dari munculnya proteinuria pada wanita hamil ialah pre-eklampsia dan eklampsia, dan dipengaruhi oleh faktor riwayat hipertensi pada orang tua. Penelitian ini bertujuan untuk mengetahui kadar protein urin pada primigravida trimester I dengan riwayat orang tua hipertensi di Kota Manado. Jenis penelitian ialah deskriptif observasional dengan desain potong lintang. Subyek penelitian diambil dengan cara non-probability sampling jenis consecutive sampling untuk mendapatkan urin dari semua subyek yang sesuai dengan kriteria dan waktu yang ditentukan. Hasil penelitian memperlihatkan bahwa dari 15 sampel urin, terdapat 9 sampel dengan positif protein (60%). Simpulan: Protein urin didapatkan pada sebagian primigravida dengan riwayat orang tua hipertensi di Kota Manado.Kata kunci: proteinuria, pre-eklampsia, riwayat hupertensi pada orang tua","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80750473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gambaran Kadar Ureum Darah pada Ibu Hamil Trimester III di Rumah Sakit Robert Wolter Mongisidi Manado","authors":"Patricia P. Matialo, Youla A. Assa, Yanti M. Mewo","doi":"10.35790/EBM.6.2.2018.22157","DOIUrl":"https://doi.org/10.35790/EBM.6.2.2018.22157","url":null,"abstract":"Abstract: Urea is waste product of protein breakdown metabolism. In the normal pregnancy, blood urea in pregnant women will decrease compared with in non-pregnant women due to physiological change of hematology, cardiovascular, and kidney hormonal in pregnancy. This study was aimed to obtain the blood urea profile of 3rd trimester of pregnancy. This was a descriptive study with a cross-sectional design, using total sampling technique. Subjects were 3rd trimester pregnant women who came to Robert Wolter Mongisidi Hospital Manado from October to November 2018. There were 39 subjects in this study. Blood urea examination showed that 39 subjects (100%) had normal blood urea levels. Conclusion: All 3rd trimester pregnant women had normal blood urea level.Keyword: blood urea level, pregnancy Abstrak: Urea adalah sisa-sisa produk metabolisme dari pemecahan protein. Pada kehamilan normal, kadar urea dalam darah akan menurun dibandingkan dengan yang tidak hamil disebabkan oleh perubahan fisiologik terutama hematologi, kardiovaskular, hormonal pada ginjal yang terjadi selama kehamilan. Penelitian ini bertujuan untuk mendapatkan gambaran kadar ureum darah pada ibu hamil trimester III. Jenis penelitian ialah deskriptif dengan desain potong lintang, dilaksanakan selama bulan Oktober – November 2018 di Rumah Sakit Robert Wolter Mongisidi Manado. Pengambilan sampel dilakukan menggunakan metode total sampling. Terdapat 39 ibu hamil trimester III sebagai subyek penelitian. Pada pemeriksaan kadar ureum didapatkan 39 subyek (100%) mempunyai kadar ureum normal. Simpulan: Kadar ureum darah pada semua ibu hamil trimester III dalam batas normal.Kata kunci: ureum, kehamilan","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"233 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77489621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Pemberian Coklat (Theobroma cacao) terhadap Kualitas Spermatozoa Tikus Wistar (Rattus norvegicus) yang Terpapar Stres","authors":"Giovanna Kurnijuanto, J. Rumbayan, Lydia E. N. Tendean","doi":"10.35790/EBM.6.2.2018.22158","DOIUrl":"https://doi.org/10.35790/EBM.6.2.2018.22158","url":null,"abstract":"Abstract: A chocolate compound, flavonoid which is a subgroup of polyphenols, has protective effect on spermatozoa exposed to stress. The flavonoid compound in chocolate, (-)-epicatehin, plays important roles in neutralizing ROS, blocking the production of ROS, and inhibiting enzyme activity of DNA methyltransferase. Stress activates the hypothalamus-pituitary-adrenal (HPA) and simpatoadrenomedular systems that have glucocorticoid as their end product that can cause an increase in essential catabolism and trigger the production of ROS. Oxidative stress can reduce the motility of spermatozoa and cause damage to DNA of spermatozoa. This study was aimed to determine the effect of chocolate (Theobroma cacao) on the quality of spermatozoa in stressed Wistar rats (Rattus norvegicus). This was an experimental study with a post-test only control group design. Samples were spermatozoa of Wistar rats. There were 9 Wistar rats divided into 3 groups, as follows: P0 (control), P1 (stress treatment only), and P2 (stress treatment and chocolate administration). The results showed that there were no significant differences in concentration and motility, albeit, there was a significant difference in morphology of spermatozoa in Wistar rats (Rattus norvegicus). Conclusion: Chocolate (Theobroma cacao) can influence the increase of morphologic quality of spermatozoa in stressed Wistar rats.Keywords: chocolate, stress, spermatozoa Abstrak: Kandungan coklat, yaitu flavonoid merupakan subgrup dari polifenol yang memiliki efek protektif terhadap spermatozoa dari paparan stres. Jenis flavonoid dalam coklat, (-)-epicatehin berperan dalam menetralisir ROS, memblokir produksi ROS, dan menginhibisi aktivitas enzim DNA methyltransferase. Stres mengaktifkan sistem hipotalamus-pituitari-adrenal (HPA) dan simpatoadrenomedular yang memiliki produk akhir glukokortikoid yang dapat menyebabkan peningkatan katabolisme esensial dan memicu produksi ROS. Stres oksidatif dapat menurunkan motilitas spermatozoa dan menyebabkan kerusakan DNA spermatozoa. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian coklat (Theobroma cacao) terhadap kualitas spermatozoa tikus Wistar (Rattus norvegicus) yang terpapar stres. Jenis penelitian ialah eksperimental dengan post-test only control group design. Sampel penelitian ialah spermatozoa tikus Wistar (Rattus norvegicus). Dilakukan perlakuan terhadap 9 ekor tikus Wistar yang dibagi menjadi 3 kelompok, yaitu P0 (kontrol), P1 (perlakuan stres saja), dan P2 (perlakuan stres dan pemberian coklat). Hasil penelitian tidak mendapatkan perbedaan bermakna pada konsentrasi dan motilitas tetapi terdapat perbedaan bermakna pada morfologi spermatozoa tikus Wistar (Rattus norvegicus). Simpulan: Pemberian coklat (Theobroma cacao) berpengaruh terhadap peningkatan kualitas morfologi spermatozoa tikus Wistar (Rattus norvegicus) yang terpapar stres.Kata kunci: coklat, stres, spermatozoa","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86574604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gambaran dan Rasionalitas Penggunaan Antibiotik pada Pasien Dewasa dengan Community Acquired Pneumonia di RSUP Prof. Dr. R. D. Kandou Manado Periode Juni 2017-Mei 2018","authors":"D. Prakoso, J. Posangi, Edward Nangoy","doi":"10.35790/EBM.6.2.2018.22156","DOIUrl":"https://doi.org/10.35790/EBM.6.2.2018.22156","url":null,"abstract":"Abstract: Irrational utilization of antibiotic led to several burdens for healthcare providers, one of them is antibiotic resistance. Community acquired pneumonia (CAP) has increased mortality rate due to irrational antibiotic utilization. This study was aimed to obtain a general depiction and antibiotic rational utilization quantitatively assessed of CAP in adult patients at Prof. Dr. R. D. Kandou Hospital Manado from June 2017 to May 2018. This was a retrospective descriptive study with a cross sectional design. Samples were 42 patients with CAP obtained by using simple random sampling. The results showed that values of DDD/100 inpatient days were, as follows: beta-lactam (33), macrolides (13.758), and fluoroquinolone (20.072). According to the ratio between estimated DDD value of Prof. Dr. R. D. Kandou and DDD WHO, all prescribed antibiotics had DDD values below or close to the value of DDD WHO. Albeit, there were discrepancies between antibiotic utilization in the field and reccomendation of Clinical Practice Guideline of Internal Medicine Department. Conclusion: Within the period of June 2017 - May 2018 the most prescribed antibiotic classes for CAP in adult patients at Prof. Dr. R. D. Kandou Hospital were beta-lactam, macrolides, and fluoroquinolone meanwhile the most prescribed antibiotics were ceftriaxone and azithromycin. In general, drug utilization was rational assessed quantitatively by using DDD WHO criteria.Keywords: antibiotic rationality, CAP, DDD WHO, Prof. Dr. R. D. Kandou Abstrak: Penggunaan antibiotik yang tidak rasional dapat membebani tenaga kesehatan, salah satunya ialah resistensi antibiotik. Community acquired pneumonia (CAP) mengalami peningkatan mortalitas tinggi akibat penggunaan antibiotik yang tidak tepat. Penelitian ini bertujuan untuk mendapatkan gambaran umum dan penilaian rasionalitas secara kuantitatif dari penggunaan antibiotik pada pasien dewasa dengan CAP di RSUP Prof. Dr. R. D. Kandou Manado periode Juni 2017-Mei 2018. Jenis penelitian ialah deskriptif retrospektif dengan desain potong lintang. Sampel sebanyak 41 pasien dewasa dengan CAP diambil dengan simple random sampling. Hasil penelitian mendapatkan DDD/100 hari rawat inap penggunaan antibiotik dari tiga golongan antibiotik yaitu beta-lactam (33), makrolida (13,758), dan florokuinolon (20,072). Berdasarkan rasio estimasi DDD di RSUP Prof. Dr. R. D. Kandou Manado dan DDD WHO seluruh obat yang digunakan masih berada di bawah DDD WHO atau mendekati nilai tersebut. Terdapat perbedaan antara penggunaan antibiotik di lapangan dan rekomendasi dari Panduan Praktek Klinis dari Bagian Ilmu Penyakit Dalam. Simpulan: Pada periode Juni 2017 - Mei 2018 golongan antibiotik yang paling banyak diberikan untuk pasien dewasa dengan CAP di RSUP Prof. Dr. R. D. Kandou Manado ialah beta-lactam, diikuti makrolida dan florokuinolon sedangkan antibiotik yang paling banyak diberikan ialah ceftriaxone dan azithromisin. Secara keseluruhan penggunaan obat sudah rasional secara kuan","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79564053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gambaran Tingkat Kecemasan pada Warga yang Tinggal di Daerah Rawan Longsor di Kelurahan Ranomuut Kota Manado","authors":"Nikhita F. A. Mamesah, Hendri Opod, Lydia E. V. David","doi":"10.35790/EBM.6.2.2018.22108","DOIUrl":"https://doi.org/10.35790/EBM.6.2.2018.22108","url":null,"abstract":"Abstract: Anxiety is a normal reaction that helps human to deal with danger or tough situation. Anxious people feel afraid, worry, and have physical changes such as increased blood pressure. This condition can happen especially among people who live in the landslide-prone area. They always feel worried and anxious when rain as well as earthquake or any condition that can trigger landslide occurs. This study was aimed to determine the anxiety level among residents living in the landslide prone-area Ranomuut Manado. This was a descriptive study with a cross sectional approach. Samples were obtained by using the purposive sampling technique. Respondents were residents of Ranomuut Lingkungan IV Manado. The anxiety level was measured with Hamilton Anxiety Rating Scale (HAR-S) questionnaire. The results showed that there were 43 respondents who participated in this study; 22 females (51.1%) and 21 males (48.9%). The anxiety levels were mild anxiety in 11 respondents (25.6%), moderate anxiety in 22 respondents (51.1%), severe anxiety in 8 respondents (18.6%), and no anxiety in 2 respondents (4.7%). Conclusion: Most residents in landslide prone-area had anxiety, and the most common anxiety was moderate anxiety.Keywords: anxiety, landslide prone-area, Hamilton Anxiety Rating Scale (HAR-S) Abstrak : Kecemasan adalah reaksi normal yang membantu manusia menghadapi situasi yang berbahaya atau sulit. Kecemasan merupakan suatu perasaan yang sifatnya umum, dimana seseorang merasa ketakutan, memiliki pikiran yang khawatir, dan perubahan fisik seperti tekanan darah yang meningkat. Keadaan ini dapat terjadi khusunya bagi orang yang tinggal di daerah rawan longsor yang akan selalu waspada dan juga merasa cemas disaat hujan, gempa atau hal-hal yang dapat memicu terjadinya longsor. Penelitian ini bertujuan untuk mengetahui gambaran tingkat kecemasan pada warga yang tinggal di daerah rawan longsor di Kelurahan Ranomuut, Kota Manado. Jenis penelitian ialah deskriptif dengan desain potong lintang. Teknik pengambilan sampel menggunakan purposive sampling. Responden penelitian ialah warga Kelurahan Ranomuut Lingkungan IV. Tingkat kecemasan diukur menggunakan kuesioner Hamilton Anxiety Rating Scale (HAR-S). Hasil penelitian mendapatkan bahwa responden yang mengikuti penelitian berjumlah 43 orang, terdiri dari perempuan 22 orang (51,1%) dan laki-laki 21 orang (48,9%). Tingkat kecemasan yang didapatkan ialah kecemasan ringan sebanyak 11 orang (25,6%), kecemasan sedang sebanyak 22 orang (51,1%), kecemasan berat sebanyak 8 orang (18,6%), dan yang tidak memiliki kecemasan sebanyak 2 orang (4,7%). Simpulan: Sebagian besar masyarakat yang berdiam di daerah rawan longsor mengalami kecemasan, terutama kecemasan sedang.Kata kunci: kecemasan, daerah rawan longsor, Hamilton Anxiety Rating Scale (HAR-S)","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83617485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluasi kualitas skenario Problem-Based Learning di Fakultas Kedokteran Universitas Sam Ratulangi","authors":"Yanti M. Mewo, F. Manoppo, H. Wungouw","doi":"10.35790/ebm.6.2.2018.20967","DOIUrl":"https://doi.org/10.35790/ebm.6.2.2018.20967","url":null,"abstract":"Abstract: Implementation of Problem Based Learning (PBL) in a tutorial discussion is not separable from the role of tutorial problems which are used to trigger the learning process. Excellent problems are needed for the success of PBL. The evaluation of PBL problems can provide an overview of strengths and weakness as well as inputs for improving the quality of problems. This study was aimed to evaluate the quality of PBL problems in Faculty of Medicine, Sam Ratulangi University Manado. We used modified questionnaire of Munshi et al. Respondents were asked to answer Likert scale questionnaires which consisted of 18 items added with one item for the overall rating of the PBL problem. The results showed that of a total of 6 problems evaluated by students and tutors, both tutors and students agreed that the weakest aspect was that the problem did not phrase to student perception of their own environment and culture (item 11). The strongest aspect identified by tutors was students were familiar with part of the knowledge necessary for discussing the problem (item 18). The strongest aspect according to students was related to the linkage of the problems with the future profession (item 13). Conclusion: The PBL problems in Faculty of Medicine, Sam Ratulangi University still needed to be improved. Some aspects had been identified as strengths and weaknesses; those could be used to improve the quality of problems.Keywords: Problem Based Learning, PBL problems, PBL tutorial Abstrak: Penerapan PBL dalam diskusi tutorial tidak lepas dari peran skenario berisikan masalah pasien yang digunakan sebagai pemicu pembelajaran mahasiswa. Skenario PBL yang memiliki kualitas baik sangat penting bagi keberhasilan pembelajaran dengan metode PBL. Evaluasi skenario PBL dapat memberikan gambaran kekuatan dan kelemahan yang dapat dijadikan sebagai masukan untuk meningkatkan kualitas skenario PBL. Penelitian ini bertujuan untuk mengevaluasi kualitas skenario PBL di Fakultas Kedokteran Universitas Sam Ratulangi (FK Unsrat) Manado. Instrumen yang digunakan ialah kuesioner dari Munshi et al. Responden diminta memberikan penilaian 18 pernyataan dan satu pertanyaan penilaian secara umum berdasarkan skala Likert. Enam skenario PBL dinilai oleh tutor dan mahasiswa. Hasil penelitian mendapatkan bahwa baik tutor maupun mahasiswa setuju bahwa yang menjadi kelemahan berkaitan dengan skenario PBL yang disusun tidak sesuai dengan wawasan mahasiswa tentang lingkungan dan kebudayaan (item 11). Menurut tutor, yang menjadi kelebihan yaitu mahasiswa telah mengenal sebagian pengetahuan yang diperlukan untuk mendiskusikan skenario (item 18). Evaluasi oleh mahasiswa didapatkan bahwa skenario menunjukkan hubungan jelas dengan profesi di masa depan merupakan kelebihan dari skenario PBL yang digunakan di FK Unsrat (item 13). Simpulan: Skenario PBL di FK Unsrat masih memerlukan perbaikan. Didapatkan beberapa aspek yang diidentifikasi sebagai kekuatan dan kelemahan yang dapat dipakai untuk meningkat","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"151 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83112669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efek Pemberian Ekstrak Daun Sirsak (Annona muricata L.) terhadap Gambaran Histopatologik Hati Tikus Wistar (Rattus Norvegicus) yang Diinduksi Rifampisin","authors":"Vega F. S. Parapaga, Meilany F. Durry, Poppy M. Lintong","doi":"10.35790/ebm.6.2.2018.22173","DOIUrl":"https://doi.org/10.35790/ebm.6.2.2018.22173","url":null,"abstract":"Abstract: Soursop leaves can be used as hepatoprotector because its flavonoid content has antioxidant activity that can inhibit and prevent liver damage. This study was aimed to obtain the effects of administration of soursop leaf extract on the histopathological features of the liver of Wistar rat induced by rifampicin. This was an experimental study using 20 Wistar rats divided into four groups. The negative control (A) group were not given any treatment; the B group was given rifampicin 1g/kgBW/day for 14 days; the C group was given rifampicin 1g/kgBW/day for 14 days followed by the administration of soursop leaf extract 400mg/kgBW/day for the next 7 days; and the D group was given rifampicin 1g/kgBW/day for 14 days followed by the administration of soursop leaf extract 600mg/kgBW/day for the next 7 days. The A and B groups were terminated at day 15, while the C and D groups were terminated at day 22. The microscopic examination revealed that the NC group had normal liver feature. The B and C groups exhibited the presence of necrotic liver cells and cloudy swelling. Meanwhile, the D group showed regenerating liver cells. Conclusion: The liver of Wistar rats that were given soursop leaf extract dose 600mg/kgBW/day after rifampicin induction showed regenerating liver cells.Keywords: rifampicin, soursop leaves, liver, histopathologic Abstrak: Daun sirsak dapat dimanfaatkan sebagai hepatoprotektor karena kandungan flavonoidnya memiliki aktivitas antioksidan yang mampu menghambat dan mencegah kerusakan oksidatif hati. Penelitian ini bertujuan untuk mengetahui efek pemberian ekstrak daun sirsak terhadap gambaran histopatologik hati tikus Wistar yang diinduksi rifampisin. Jenis penelitian ialah eksperimental, menggunakan 20 ekor tikus Wistar yang dibagi dalam empat kelompok. Kelompok kontrol negatif (A) tidak diberi perlakuan selama 14 hari; kelompok B diberi rifampisin 1g/kgBB/hari selama 14 hari; kelompok C diberi rifampisin 1g/kgBB/hari selama 14 hari dilanjutkan dengan pemberian ekstrak daun sirsak 400mg/ kgBB/hari selama 7 hari; dan kelompok D diberi rifampisin 1g/kgBB/hari selama 14 hari dilanjutkan dengan pemberian ekstrak daun sirsak 600mg/kgBB/hari selama 7 hari. Tikus kelompok A dan B diterminasi pada hari ke-15, sedangkan kelompok C dan D diterminasi pada hari ke-22. Hasil penelitian menunjukkan gambaran histopatologik hepar tikus Wistar yang normal pada kelompok A, sedangkan pada kelompok B dan C tampak adanya sel-sel hati yang mengalami nekrosis dan degenerasi bengkak keruh. Pada kelompok D terlihat adanya sel-sel hati yang mengalami regenerasi. Simpulan: Hati tikus Wistar yang diberikan ekstrak daun sirsak dosis 600mg/kgBB/hari setelah pemberian rifampisin menunjukkan gambaran sel-sel hati yang mengalami regenerasi.Kata kunci: rifampisin, daun sirsak, hati, gambaran histopatologik","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72954951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uji Resistensi Bakteri terhadap Arsen yang di Isolasi dari Sedimen di Muara Sungai Totok","authors":"Tessalonicha J. Rompis, Widdhi Bodhi, Fona Budiarso","doi":"10.35790/EBM.6.2.2018.21997","DOIUrl":"https://doi.org/10.35790/EBM.6.2.2018.21997","url":null,"abstract":"Abstract: Arsenic is still used in lots of human activities that results in environmental pollution. Arsenic is also found in nature in a small quantity. Gold mining industry is one of the human activities that uses arsenic. Mining waste that is not managed properly can inflict environmental pollution and harms the human health due to its toxic-substance content. This study was aimed to isolate and identify bacteria in the soil sediment obtained from the estuary area of Totok River, Southeast Minahasa and tested the bacteria's resistance levels to arsenic. Bacteria were isolated from sediment samples from the estuary area of Totok River, and the isolated bacteria were grown in agar media containing arsenic trioxide. Bacteria identification were perfomed by using morphological, physiological and biochemical tests. The results showed that bacteria grew successfully in 10 isolates; 2 isolates were Gram negative bacteria and 8 isolates were Gram positives bacteria. All isolates showed no motility. Identification of bacteria revealed four genera: Bacillus sp., Streptococcus sp., Acetobacter sp, and Escherichia sp that were resistant to arsenic 1000 ppm. Conclusion: There were 4 genera of arsenic-resistant bacteria identified in the sediment of the estuary area of Totok River, as follows: Bacillus sp., Streptococcus sp., Acetobacter sp, and Escherichia sp. All of them were resistant to arsenic 1000 ppm.Keywords: sediment, arsenic-resistant bacteria Abstrak: Arsen (As) masih banyak ditemukan dalam kegiatan manusia yang berakibat pencemaran terhadap lingkungan. Arsen juga dapat ditemukan di alam secara alami namun dalam jumlah yang sedikit. Industri pertambangan emas merupakan salah satu kegiatan manusia yang menggunakan arsen. Limbah pertambangan yang tidak dikelola dengan baik dapat mencemari lingkungan dan mengganggu kesehatan manusia karena berpotensi mengandung racun yang berbahaya. Penelitian ini bertujuan untuk mengidentifikasi bakteri resisten arsen serta tingkat resistensinya yang terdapat pada sedimen di muara Sungai Totok Kecamatan Ratatotok Kabupaten Minahasa Tenggara Sulawesi Utara. Bakteri diisolasi dari sedimen di muara sungai Totok dan ditumbuhkan pada media agar yang mengandung arsen trioksida. Identifikasi dari bakteri yang tumbuh menggunakan uji fisiologi, uji morfologi dan uji biokimia. Hasil penelitian mendapatkan 10 isolat dengan bakteri yang berhasil tumbuh, yaitu 2 isolat bakteri Gram negatif dan 8 isolat bakteri Gram positif. Semua isolat tidak menunjukkan adanya motilitas dan dapat dikategorikan ke dalam empat kelompok genus yaitu Bacillus sp., Streptococcus sp., Acetobacter sp, dan Escherichia sp. Keempat genus bakteri ini resisten terhadap arsen hingga konsentrasi 1000 ppm. Simpulan: Terdapat empat genus bakteri resisten arsen yang teridentifikasi dalam sedimen di muara Sungai Totok, yaitu Bacillus sp., Streptococcus sp., Acetobacter sp, dan Escherichia sp. Kesemuanya resisten terhadap arsen hingga konsentrasi 1000 ppm.Kata ku","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"os-12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87189180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perbandingan antara Pengaruh Olahraga Berlebihan dan Paparan Asap Rokok terhadap Kualitas Spermatozoa Tikus Wistar (Rattus norvegicus)","authors":"Vania E. Laoh, Lydia E. N. Tendean, Grace L. A. Turalaki","doi":"10.35790/ebm.6.2.2018.22112","DOIUrl":"https://doi.org/10.35790/ebm.6.2.2018.22112","url":null,"abstract":"Abstract: Excessive exercise can cause an increase in free radicals 2-3 times. Cigarette smoke can also increase free radicals and reduce antioxidants in semen, therefore, it can cause DNA damage through cellular DNA fragmentation. Free radicals can come from endogenous sources, such as the normal oxidation reaction in mitochondria, peroxisomes, whereas free radicals from exogenous sources come from cigarette smoke, inflammation, and excessive exercise training. This study was aimed to compare the effect of excessive exercise and exposure to cigarette smoke on the quality of sperm of Wistar rats (Rattus norvegicus). This was an experimental study using a post-test only control group design. Samples were 9 Wistar rats (Rattus norvegicus) divided into 3 groups: P0 group, the control group without any treatment; P1 group, treated with excessive intensity exercise; and P2 group, treated with 2-cigarette-smoke exposure. The treatment was carried out for 50 days. The results showed differences in concentration between study groups but not statistically significant (P>0.05). Motility showed significant differences in normal motility of group P0 and P1 (P=0.002), and of group P0 and P2 (P=0). Morphology showed a significant difference between groups P0 and P1 (P=0.004). Conclusion: Excessive exercise and exposure to cigarette smoke affect the quality of spermatozoa of Wistar rats (Rattus norvegicus).Keywords: excessive exercise, cigarette smoke, sperm quality Abstrak: Olahraga berlebihan dapat menyebabkan peningkatan radikal bebas sebanyak 2–3 kali. Asap rokok dapat meningkatkan radikal bebas serta menurunkan antioksidan dalam cairan semen sehingga dapat mengakibatkan kerusakan DNA melalui fragmentasi DNA seluler. Radikal bebas dapat berasal dari sumber endogen, yaitu pada reaksi reduksi oksidasi normal dalam mitokondria, peroksisom, sedangkan radikal bebas dari sumber eksogen berasal dari asap rokok, inflamasi, dan latihan olahraga berlebihan. Penelitian ini bertujuan untuk mengetahui perbandingan antara pengaruh olahraga berlebihan dan paparan asap rokok terhadap kualitas spermatozoa tikus Wistar (Rattus norvegicus). Jenis penelitian ialah eksperimental dengan post-test only control group design. Sampel penelitian sebanyak 9 ekor tikus wistar (Rattus norvegicus) dibagi menjadi 3 kelompok: kelompok P0 tidak diberikan olahraga berlebihan dan paparan asap rokok; kelompok P1 diberikan olahraga berlebihan intensitas berat; dan kelompok P2 diberikan paparan asap rokok 2 batang. Perlakuan dilakukan selama 50 hari. Hasil penelitian menunjukkan perbedaan konsentrasi antar kelompok penelitian, namun analisis statistik menunjukkan hasil yang tidak bermakna (P>0,05). Pada motilitas didapatkan hasil bermakna untuk motilitas normal dan abnormal kelompok P0 dan P1 (P=0,002), serta kelompok P0 dan P2 (P=0). Pada morfologi didapatkan hasil bermakna antara kelompok P0 dan P1 (P=0,004). Simpulan: Pemberian olahraga berlebihan dan paparan asap rokok berpengaruh terhadap k","PeriodicalId":17693,"journal":{"name":"Jurnal e-Biomedik","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84299590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}