Journal of Veterinary Research最新文献

{"title":"Determination of the rabies virus variants circulating in Poland in 2021-2023 and their phylogeny with analysis of the strains in the Mazowieckie and Podkarpackie voivodeships.","authors":"Janusz Ciołek, Anna Orłowska, Marcin Smreczak","doi":"10.2478/jvetres-2024-0032","DOIUrl":"10.2478/jvetres-2024-0032","url":null,"abstract":"<p><strong>Introduction: </strong>Rabies is endemic in Europe and red foxes are the vector and reservoir of the rabies virus (RABV). Based on classification established in the early 1990s, four variants of the rabies virus have been distinguished in Europe. Rabies broke out in January 2021 in the Mazowieckie voivodeship in central north-eastern Poland. The virus spread rapidly, reaching the Świętokrzyskie voivodeship in the central southern part and the Lubelskie voivodeship in the eastern part in the next months. Nine rabies cases were reported in the Podkarpackie voivodeship in south-eastern Poland between 2021 and 2023, mainly in red foxes but also in dogs and wildcat. The aim of the study was the identification of RABV variants in wildlife and domestic animals in Poland between 2021 and 2023.</p><p><strong>Material and methods: </strong>The study involved 157 animal brains tested positive for rabies using a fluorescent antibody test. From 10% w/v brain homogenates, RNA was isolated and full-length RABV genomes were high-throughput sequenced with an RABV-enriched approach. Complete genomes of RABV isolates were phylogenetically analysed and the variants were estimated.</p><p><strong>Results: </strong>Molecular and phylogenetic studies revealed 147 (93.6%) of the RABV strains out of 157 which had rapidly spread in the wildlife of the Mazowieckie, Świętokrzyskie and Lubelskie voivodeships to be Central European strains. Nine RABVs (5.7%) detected in foxes, a wildcat and a dog in the Podkarpackie voivodeship were identified as North-Eastern European. A vaccine-induced rabies case was detected in a red fox in the Lubelskie voivodeship in May 2023.</p><p><strong>Conclusion: </strong>Central European and North-Eastern European RABVs were circulating in Poland between 2021 and 2023.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"175-180"},"PeriodicalIF":1.3,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11212032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diagnostic value of selected immune parameters in peripheral blood of dogs with malignant mammary tumours - a preliminary study.","authors":"Urszula Lisiecka, Piotr Brodzki, Anna Śmiech, Katarzyna Michalak, Stanisław Winiarczyk, Beata Żylińska, Łukasz Adaszek","doi":"10.2478/jvetres-2024-0035","DOIUrl":"10.2478/jvetres-2024-0035","url":null,"abstract":"<p><strong>Introduction: </strong>The main adaptive immune cells are T and B lymphocytes and they play key roles in the induction of immune responses against canine mammary tumours. Investigating these cell subpopulations may lead to more precise diagnosis of these malignancies.</p><p><strong>Material and methods: </strong>The percentages of CD3⁺, CD4⁺ and CD8⁺ T cells and of CD21⁺ B cells in the peripheral blood of bitches with malignant mammary tumours were compared with those in the blood of healthy animals. The phenotypic features of peripheral blood leukocytes were evaluated by flow cytometry.</p><p><strong>Results: </strong>There was a significant difference in the mean percentages of CD3⁺ lymphocytes between healthy (66.7%) and metastatic dogs (46.1%), and between tumour-bearing non-metastatic (66.6%) and metastatic dogs. There was also a significant difference in CD4⁺ T helper cell percentages between healthy dogs (40.4%) and dogs with metastases (23.2%), and between the latter and dogs without them (35.5%). In the case of CD21⁺ lymphocyte subsets, a significant difference was noted between healthy animals (10.9%) and those with metastases (20.1%), and between the latter and patients without metastases (8.5%). There were also significant differences in CD3⁺/CD21⁺ ratios between the group with metastases (3.0), the healthy group (7.8), and the group without metastases (8.5). Similarly, a significant difference was noted in CD4⁺/CD8⁺ ratios between animals with metastases (1.4), bitches in the control group (2.2), and dogs without metastases (1.9).</p><p><strong>Conclusion: </strong>Peripheral blood leukocyte phenotypic characteristics are putative novel biomarkers. These findings may be useful in future studies improving mammary tumour diagnostic procedures, especially in metastasis detection.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"271-278"},"PeriodicalIF":1.3,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vector-borne diseases imported to Poland between 2021 and 2023.","authors":"Łukasz Adaszek, Marta Staniec, Banu Dokuzeylül, Maria Pisarek, Maciej Skrzypczak, Paweł Żółkiewski, Małgorzata Rutkowska-Szulczyk, Łukasz Deneka, Mehmet Erman Or, Stanisław Winiarczyk","doi":"10.2478/jvetres-2024-0033","DOIUrl":"10.2478/jvetres-2024-0033","url":null,"abstract":"<p><strong>Introduction: </strong>The aim of the study was to monitor the occurrence of selected vector-borne diseases in anaemic dogs arriving in or returning to Poland from areas endemic for these diseases.</p><p><strong>Material and methods: </strong>The study involved 497 dogs, of which 184 came to Poland from Ukraine with their owners fleeing the war. Other animals returned to the country from holidays spent in Croatia (n = 96), Turkey (n = 79), Italy (n = 48), Bulgaria (n = 42), Albania (n = 36) and Romania (n = 12). Molecular biology methods were used for detection of pathogens transmitted by the vectors.</p><p><strong>Results: </strong>Molecular tests revealed the presence of vector-borne pathogens in 79 dogs. The most commonly diagnosed infection was caused by <i>Babesia canis</i> (27 dogs), followed by infections with <i>Anaplasma phagocytophilum</i> (in 20 dogs), <i>Mycoplasma haemocanis</i> (15 dogs), <i>Bartonella henselae</i> (7 dogs), <i>Ehrlichia canis</i> (4 dogs), <i>Hepatozoon canis</i> (3 dogs), <i>Babesia gibsoni</i> (2 dogs) and <i>Leishmania infantum</i> (1 dog). Most of the sick dogs (n = 39) came from Ukraine. In dogs spending holidays with their owners outside Poland, vector-borne diseases were most often detected after their return from Turkey (n = 16), and next in descending order from Croatia (n = 7), Italy (n = 6), Albania (n = 4), Bulgaria (n = 4) and Romania (n = 3).</p><p><strong>Conclusion: </strong>The wider migration crisis and increasingly frequent trips of owners with their dogs to areas of endemic infectious and parasitic diseases observed in recent years are the main risk factors for the occurrence of these diseases in Poland. Therefore, constant monitoring of vector-borne diseases, especially in dogs returning from holidays and arriving in Poland from abroad, seems to be crucial for their early detection and introduction of appropriate therapy.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"215-222"},"PeriodicalIF":1.3,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chickens' eggs and the livers of farm animals as sources of perfluoroalkyl substances.","authors":"Szczepan Mikołajczyk, Małgorzata Warenik-Bany, Marek Pajurek","doi":"10.2478/jvetres-2024-0034","DOIUrl":"10.2478/jvetres-2024-0034","url":null,"abstract":"<p><strong>Introduction: </strong>This study focuses on perfluoroalkyl substance (PFAS) content in chickens' eggs and the livers of farm animals.</p><p><strong>Material and methods: </strong>Chickens' eggs (n = 25) and the livers of cows (n = 10), chickens (n = 7) and horses (n = 3) were collected from various regions of Poland. Samples were analysed using the isotope dilution technique with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).</p><p><strong>Results: </strong>The mean lower bound (LB) sum of four PFAS (∑4 PFAS) concentrations (perfluorooctanesulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA) and perfluorohexanesulfonic acid (PFHxS)) were the highest in cows' livers (0.52 μg/kg) and much lower in chickens' (0.17 μg/kg) and horses' livers (0.13 μg/kg) and chickens' eggs (0.096 μg/kg). The ratio of ∑4 PFASs to the limits set by Commission Regulation (EU) 2023/915 was <7% for liver and <6% for eggs. Linear PFOS was the compound with the highest detection frequency (8% in eggs and 48% in all livers). In cows' livers it was detected in 80% of samples. The estimated exposure to LB ∑4 PFASs <i>via</i> consumption of liver tissue from farm animals (assuming 50 g and 100 g portions) was <52% of the tolerable weekly intake (TWI) for children and <17% of the TWI for adults. Dietary intake <i>via</i> the average portion of three eggs led to low exposure of <15% for children and <5% for adults.</p><p><strong>Conclusion: </strong>Neither eggs nor the livers of chickens or horses as analysed in this study are significant sources of PFASs, while cows' livers might contribute significantly to a child's overall dietary intake. Further investigation of PFOS in farm animal livers should be conducted.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"241-248"},"PeriodicalIF":1.3,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of doxycycline residues in bones after oral administration to broiler chickens.","authors":"Anna Gajda, Iwona Szymanek-Bany, Ewelina Nowacka-Kozak, Małgorzata Gbylik-Sikorska","doi":"10.2478/jvetres-2024-0030","DOIUrl":"10.2478/jvetres-2024-0030","url":null,"abstract":"<p><strong>Introduction: </strong>Chicken bones, a by-product of the poultry industry, can directly or indirectly enter the food chain. Bone meal and bone products could be sources of many contaminants. Considering the wide range of uses made of bones in the culinary and food industries, this material needs to be safe and antibiotic residue-free. To determine if such is the case, the concentration of doxycycline in chicken bones was investigated, this antimicrobial being one of the most commonly used in poultry production.</p><p><strong>Material and methods: </strong>Ross 308 broilers were grouped into three experimental and one control group. Doxycycline was administered in drinking water at therapeutic and sub-therapeutic doses, as well as <i>via</i> spray treatment. The concentration of doxycycline in bones was determined post slaughter by ultra-high performance liquid chromatography-tandem mass spectrometry.</p><p><strong>Results: </strong>Doxycycline was quantified at 135 μg/kg 22 days after the last day of antibiotic administration at therapeutic doses; 2,285 μg/kg after sub-therapeutic treatment for 27 days and 9.62 μg/kg 22 days after the end of spray application.</p><p><strong>Conclusion: </strong>High concentrations and long persistence of doxycycline in bones were found in this study. Doxycycline can contaminate all bone-derived products in the food and fertiliser industries.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"255-261"},"PeriodicalIF":1.3,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141474659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of transfer of different sulphonamides from contaminated beeswax to honey.","authors":"Kamila Mitrowska, Maja Antczak","doi":"10.2478/jvetres-2024-0029","DOIUrl":"10.2478/jvetres-2024-0029","url":null,"abstract":"<p><strong>Introduction: </strong>No maximum residue limits in honey have been legislated in the EU for antimicrobial substances such as sulphonamides, and they are not permitted, therefore, for treating honey bees unless in a cascade system. Since sulphonamides are used illegally in apiculture to treat foulbrood, their residues can be found in honey and other apiculture products, including beeswax. The study aimed to assess the contamination of honey from beeswax containing residues of 10 sulphonamides (sulphadimethoxine (SDM), sulphadoxine (SDX), sulphamonomethoxine (SMM), sulphamethoxazole (SMX), sulphameter (SMT), sulphamethazine (SMZ), sulphamerazine (SMR), sulphadiazine (SDA), sulphathiazole (STZ) and sulphacetamide (SCA)).</p><p><strong>Material and methods: </strong>Wax-based foundations fortified with 10 sulphonamides at 10,000 μg/kg were evaluated for sulphonamide concentrations and then placed in a beehive so that honey bees (<i>Apis mellifera</i> L.) could build honeycombs with them. Frames of capped honey were taken out of the hives one month later and honey was sampled from them. The honeycombs were subsequently incubated in a laboratory at 35°C for five months, and honey was sampled monthly. The honey sulphonamide concentrations were measured using liquid chromatography-tandem mass spectrometry and compared to the wax-based foundation concentrations.</p><p><strong>Results: </strong>The maximum transfers to honey of the initial amount of SDM, SDX, SMM, SMX, SMT, SMZ, SMR, SDA, STZ and SCA in the wax-based foundations were 42.6, 34.3, 31.7, 30.1, 29.5, 25.2, 18.7, 16.1, 9.5 and 8.6%, respectively.</p><p><strong>Conclusion: </strong>This study demonstrated that every tested sulphonamide could migrate from beeswax in antimicrobial-tainted honeycombs to honey, SDM having the highest migration potential and SCA the lowest.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"249-254"},"PeriodicalIF":1.3,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles in cattle infected with bovine leukaemia virus: isolation and molecular analysis.","authors":"Maria Szczotka, Magdalena Wasiak, Jacek Kuźmak","doi":"10.2478/jvetres-2024-0031","DOIUrl":"10.2478/jvetres-2024-0031","url":null,"abstract":"<p><strong>Introduction: </strong>Exosomes are nanosized lipid bilayer membranous microvesicles, extracellularly released from a variety of mammalian cells. They mediate intercellular signalling by transporting several types of RNA, lipids and proteins and participate in the intercellular exchange of DNA, RNA, micro RNA, proteins and other components. These microvesicles are present in all body fluids in physiological and pathological conditions and reflect the state of the host organism. The aim of the study was the isolation and molecular determination of exosomes in blood and supernatant fluids of bovine dendritic cell cultures infected with bovine leukaemia virus (BLV).</p><p><strong>Material and methods: </strong>Exosomes were isolated by ultracentrifugation from the blood sera, plasma and supernatant of bovine BLV-infected and uninfected control dendritic cell cultures and their presence was confirmed with scanning electron and transmission electron microscopy. Western blot analysis of the structural BLV glycoprotein 51 (Env) and protein 24 (Gag) and of the tetraspanin exosomal markers CD9, CD63 and flotillin-1 was undertaken in BLV+ and control BLV- cattle.</p><p><strong>Results: </strong>In exosomes of leukaemic cattle both BLV proteins and exosomal markers were detected. In healthy control animals only exosomal markers were determined.</p><p><strong>Conclusion: </strong>Proteins of BLV were released with exosomes and could be transferred into recipient cells as an alternative propagation route not requiring virus infection.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"189-198"},"PeriodicalIF":1.3,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses.","authors":"Monika Olech, Jacek Kuźmak","doi":"10.2478/jvetres-2024-0025","DOIUrl":"10.2478/jvetres-2024-0025","url":null,"abstract":"<p><strong>Introduction: </strong>Small ruminant lentiviruses (SRLV) cause multisystemic, degenerative and chronic disease in sheep and goats. There are five genotypes (A, B, C, D and E), of which A and B are the most widespread. The purpose of this study was to evaluate the serotyping efficiency of the Eradikit SRLV Genotyping ELISA and the molecular typing efficiency of a newly developed nested real-time PCR targeting the long terminal repeat-<i>gag</i> (LTR-gag) region using samples from animals infected with subtypes of SRLV known to circulate in Poland.</p><p><strong>Material and methods: </strong>A total of 97 sera samples taken from 34 sheep and 63 goats were immunoassayed, and 86 DNA samples from 31 sheep and 55 goats were tested with the PCR. All ruminants were infected with known SRLV strains of the A1, A5, A12, A13, A16, A17, A18, A23, A24, A27, B1 and B2 subtypes.</p><p><strong>Results: </strong>A total of 69 (80.2%, 95% confidence interval 71.6%-88.8%) out of 86 tested samples gave positive results in the PCR. In 17 out of the 86 (19.8%) samples, no proviral DNA of SRLV was detected. The differentiation between MVV (genotype A) and CAEV (genotype B) by PCR matched the predating phylogenetic analysis invariably. No cross-reactivity was observed. On the other hand, the proportion of samples genotyped the same by the older phylogenetic analysis and the Eradikit SRLV Genotyping ELISA was 42.3%. The test was unable to classify 40.2% of samples, and 17.5% of sera were incorrectly classified.</p><p><strong>Conclusion: </strong>Our results showed that the Eradikit SRLV genotyping kit is not a reliable method for predicting SRLV genotype, while the nested real-time PCR based on the LTR-<i>gag</i> region did prove to be, at least for genotypes A and B.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"181-188"},"PeriodicalIF":1.3,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic analysis of the trypanosomatid parasite <i>Lotmaria passim</i> in honey bees (<i>Apis mellifera</i>) in Poland.","authors":"Maria Iller, Karolina Lipczyńska-Ilczuk, Rajmund Sokół, Grzegorz Borsuk, Agata Bancerz-Kisiel","doi":"10.2478/jvetres-2024-0018","DOIUrl":"10.2478/jvetres-2024-0018","url":null,"abstract":"<p><strong>Introduction: </strong><i>Lotmaria passim</i> (<i>L. passim</i>) is a single-celled flagellate which colonises the bee gastrointestinal tract and is highly prevalent in honey bees. This parasite is associated with colony losses. Honey bee (<i>Apis mellifera</i>) colonies were sampled from five apiaries in the north-eastern part of Poland for the phylogenetic analysis of <i>L. passim</i>.</p><p><strong>Material and methods: </strong>Each apiary consisted of approximately 60 bee colonies, of which 20 were randomly selected. Samples of 60 differently aged worker bees were collected from each colony and pooled. A total of 100 bee colonies from five apiaries were examined. Protozoa of the Trypanosomatidae family were identified by PCR. <i>L. passim</i> was detected in 47 (47%) of the samples. The 18S ribosomal (r) RNA amplicons of <i>L. passim</i> were sequenced by a commercial service. Their sequences were analysed with BLASTN and noted to be compatible with the GenBank sequences of this region of the organism's genome. A sequence analysis was performed using the BioEdit Sequence Alignment Editor and Clustal W software.</p><p><strong>Results: </strong>The amplicon sequences of <i>L. passim</i> were 100% homologous with the sequences deposited in GenBank under accession numbers KM066243.1., KJ684964.1 and KM980181.1.</p><p><strong>Conclusion: </strong>This is the first study to perform a phylogenetic analysis of <i>L. passim</i> in Polish honey bees. The analysis demonstrated high levels of genetic similarity between isolates of <i>L. passim</i> colonising apiaries in the north-eastern region of Poland.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 1","pages":"123-127"},"PeriodicalIF":1.3,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first confirmed cases of pigeon rotavirus A (RVA) infection in domestic pigeons <i>(Columba livia)</i> in Poland.","authors":"Krzysztof Adamczyk, Dennis Rubbenstroth, Aleksandra Ledwoń, Rafał Sapierzyński, Piotr Szeleszczuk","doi":"10.2478/jvetres-2024-0006","DOIUrl":"10.2478/jvetres-2024-0006","url":null,"abstract":"<p><strong>Introduction: </strong>Although the presence of rotaviruses in pigeon samples has been reported since the 1980s, its importance as an aetiological agent of the \"classical\" young pigeon disease (YPD) was not proven until 2020, when the Henle-Koch postulates were confirmed for pigeon-type rotavirus A (RVA) genotype G18P(17).</p><p><strong>Material and methods: </strong>From 2011 to 2020, archived liver samples from 117 pigeons submitted by 74 individual lofts were tested for the presence of pigeon-type RVA using a VP6-specific RT-qPCR test. For four positive racing pigeons, a more detailed necropsy and histopathological analysis was performed.</p><p><strong>Results: </strong>Indicators of an acute RVA infection were found in 24 out of 117 (20.5%) samples tested, the earliest in 2014. Necropsies of the four selected RVA-positive pigeons showed changes mainly in the liver, spleen and kidneys similar to those described by other researchers. The histopathological examination revealed mainly hyperaemia and necrosis in the liver, as well as mononuclear cell infiltrates in these organs.</p><p><strong>Conclusion: </strong>Pigeon-type RVA is also a cause of YPD in Poland and is a serious challenge for racing pigeon breeders and veterinarians, especially during the training and flights of young pigeons.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 1","pages":"55-61"},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}