Journal of Veterinary Research最新文献_第7页

Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6). 开发基于重组蛋白的酶联免疫吸附试验，用于检测牛疱疹病毒 6 (BoHV6) 抗体。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2023-12-19 eCollection Date: 2023-12-01 DOI: 10.2478/jvetres-2023-0069

Piotr Kubiś, Jacek Kuźmak

{"title":"Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6).","authors":"Piotr Kubiś, Jacek Kuźmak","doi":"10.2478/jvetres-2023-0069","DOIUrl":"10.2478/jvetres-2023-0069","url":null,"abstract":"Introduction: Bovine herpesvirus 6 (BoHV6) belongs to the Herpesviridae family, Gammaherpesvirinae subfamily and Macavirus genus. It is common in cattle, but was also detected in American bison (Bison bison) and water buffalo (Bubalus bubalis). The aim of the experiment was to develop an ELISA for serological examination of cattle sera for the presence of anti-BoHV6 specific antibodies.Material and methods: Viral DNA from a BoHV6-positive cow was amplified by qPCR and the resulting fragments of the gB and gH genes encoding glycoproteins B and H (gB and gH) were cloned into the pLATE52 vector to express recombinant gB (rgB) and gH (rgH) in Rosetta (DE3) E. coli. The expressed recombinant proteins were used as antigens in the developed ELISA.Results: The proteins expressed had the expected molecular weight. A total of 143 sera were examined, and 141 of them were positive, according to the chosen cut-off values of 9% and 10% for the sample-to-positive ratios of the rgB and rgH antigens, respectively.Conclusion: The rgB and rgH recombinant antigens of BoHV6 were successfully expressed in E. coli and successfully used in a newly developed ELISA.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoreactivity of p21, MMP-1 and CB2 receptor proteins in cutaneous canine mast cell tumours: an association with the three-tier grading system. 皮肤犬肥大细胞瘤中 p21、MMP-1 和 CB2 受体蛋白的免疫反应：与三级分级系统有关。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2023-12-19 eCollection Date: 2023-12-01 DOI: 10.2478/jvetres-2023-0066

Kamila Bulak, Anna Kycko, Anna Śmiech, Wojciech Łopuszyński

{"title":"Immunoreactivity of p21, MMP-1 and CB2 receptor proteins in cutaneous canine mast cell tumours: an association with the three-tier grading system.","authors":"Kamila Bulak, Anna Kycko, Anna Śmiech, Wojciech Łopuszyński","doi":"10.2478/jvetres-2023-0066","DOIUrl":"10.2478/jvetres-2023-0066","url":null,"abstract":"Introduction: Mast cell tumours (MCTs) arise in the dermis and subcutaneous tissues in animals and humans and are one of the most common neoplasms of the skin in dogs. Cannabinoid type 2 receptor (CB2R), cyclin-dependent kinase inhibitor (p21) and matrix metalloproteinase 1 (MMP-1) are potential targets for novel anti-tumour therapeutic strategies. This study evaluated by immunohistochemical means the reactivity of p21, MMP-1 and CB2R proteins in association with a three-tier grading system in cutaneous canine MCTs.Material and methods: Formalin-fixed, paraffin-embedded canine MCTs were processed for histochemical analysis and immunohistochemical staining using antibodies against p21, MMP-1 and CB2R. The results were analysed statistically.Results: The strongest p21 immunolabelling was detected in grade 3 MCTs, while grade 1 tumours showed mild or no detectable p21 immunoreactivity (P-value < 0.001). Strong immunolabelling of MMP-1 was the most common in grade 1 tumours (P-value < 0.001) and CB2R was significantly less frequent in grade 3 tumours than in grade 1 (P-value < 0.001) and grade 2 (P-value < 0.001).Conclusion: High immunoreactivity of MMP-1 can be a marker of grade 1 MCTs in dogs, whereas p21 protein overexpression can be a marker of grade 3 canine MCTs. Strong CB2R immunoreactivity with simultaneous underexpression of p21 and high immunoreactivity of MMP-1 proteins may indicate that the use of cannabinoids in grade 1 MCTs in dogs is practicable.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Why does Listeria monocytogenes survive in food and food-production environments? 为什么李斯特菌能在食品和食品生产环境中存活？

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2023-12-02 DOI: 10.2478/jvetres-2023-0068

J. Osek, K. Wieczorek

引用次数: 0

New insight on chlamydiae 对衣原体的新认识

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2023-12-02 DOI: 10.2478/jvetres-2023-0067

M. Szymańska-Czerwińska, Kinga Zaręba-Marchewka, K. Niemczuk

引用次数: 0

Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland 波兰西部生乳牛乳中选定microrna的微滴数字PCR定量分析

3区农林科学

Journal of Veterinary Research Pub Date : 2023-11-08 DOI: 10.2478/jvetres-2023-0062

Sebastian Smulski, Marcin Pszczoła, Monika Stachowiak, Adrianna Bilińska, Izabela Szczerbal

{"title":"Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland","authors":"Sebastian Smulski, Marcin Pszczoła, Monika Stachowiak, Adrianna Bilińska, Izabela Szczerbal","doi":"10.2478/jvetres-2023-0062","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0062","url":null,"abstract":"Abstract Introduction MicroRNAs (miRNAs), a class of noncoding small RNAs, have been recognised as potential biomarkers of mammary gland conditions, including bovine mastitis diagnosis. The aim of this study was to quantify selected miRNAs in the milk of mastitic cows. Material and Methods Milk samples (n = 90) were collected from healthy and mastitic dairy cows originating from local dairy cattle farms located in the west of Poland. MicroRNAs of the miR-21a, miR-92a, miR-146a and miR-383 species were quantified using the highly sensitive droplet digital PCR method. Direct measurement of somatic cell count (SCC) was performed using a cell counter. Cows were divided into three groups: those with an SCC below 200,000/mL were designated Low (n = 25), those with an SCC between 200,000 and 999,999 were Medium (n = 34), and those with an SCC of 1,000,000 or higher were High (n = 31). Microbiological analyses were performed using standard culture testing. Results The level of miR-383 was very low and this miRNA was excluded from analysis. The miR-92a was used to normalise miR-21a and miR-146a expression levels. The obtained results of expression of miR-21a and miR-146a correlated with somatic cell number (R = 0.53 and 0.79, respectively). Conclusion These results show that ddPCR is a useful method for quantifying miRNAs in raw cow milk. It seems that miR-146a is a promising marker for bovine mastitis, although further studies are needed to select a panel of miRNAs that can be used in mastitis monitoring in Poland.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135342246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the expression of testin in the testes of dogs 犬睾丸中睾酮表达的研究

3区农林科学

Journal of Veterinary Research Pub Date : 2023-10-31 DOI: 10.2478/jvetres-2023-0055

Rafał Ciaputa, Marcin Nowak, Stanisław Dzimira, Eleonora Brambilla, Małgorzata Kandefer-Gola, Alicja Tomaszek, Aneta Popiel-Kopaczyk, Piotr Dzięgiel, Valeria Grieco

{"title":"Study on the expression of testin in the testes of dogs","authors":"Rafał Ciaputa, Marcin Nowak, Stanisław Dzimira, Eleonora Brambilla, Małgorzata Kandefer-Gola, Alicja Tomaszek, Aneta Popiel-Kopaczyk, Piotr Dzięgiel, Valeria Grieco","doi":"10.2478/jvetres-2023-0055","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0055","url":null,"abstract":"Abstract Introduction Testin is a protein involved in cell mobility, adhesion and colony formation. In rats, testin presence has been reported in the testes, and its possible role in spermatogenesis has been suggested. Studies in humans also suggest a possible role of testin as a cancer suppressor protein. In the dog, which represents both an important pet species and a good animal model for studying biological and pathological testicular processes, the presence of testin has never been reported. Material and Methods In the present study, the expression of testin in foetal, prepubertal, adult and aged canine testes was investigated. Testes from 5 adult and 3 aged dogs, from 2 one-month-old puppies and from 2 foetuses miscarried at the end of pregnancy were immunohistochemically examined with a commercial antibody against testin. Results Testin was intensely expressed in Sertoli cells in every testis examined. Spermatids were also positive for testin in mature dogs and in the testicular areas of the aged ones which were not atrophic. Weak expression of testin was also detected in all testes examined. Conclusion The present study, the first demonstrating the presence of testin in canine testes, provides the basis for further dog–human comparative research and for studies on the role of this protein in canine physiology, reproduction and testicular pathologies.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135872169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of plant extracts and bacteriophages as an alternative therapy approach in combatting bacterial infections: the study of lytic phages and Stevia rebaudiana 利用植物提取物和噬菌体作为对抗细菌感染的替代疗法:溶噬菌体和甜菊糖的研究

3区农林科学

Journal of Veterinary Research Pub Date : 2023-10-24 DOI: 10.2478/jvetres-2023-0059

Xymena Stachurska, Małgorzata Mizielińska, Magdalena Ordon, Paweł Nawrotek

{"title":"The use of plant extracts and bacteriophages as an alternative therapy approach in combatting bacterial infections: the study of lytic phages and Stevia rebaudiana","authors":"Xymena Stachurska, Małgorzata Mizielińska, Magdalena Ordon, Paweł Nawrotek","doi":"10.2478/jvetres-2023-0059","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0059","url":null,"abstract":"Abstract Introduction In the light of the problem of antibiotic resistance, the use of combined alternative therapies in combatting bacteria-related disorders has gained popularity. Bacteriophages are one element implemented in new combination therapy. Stevia rebaudiana is known to have antimicrobial activity and regarded as potentially having a synergistic effect with bacteriophages. Therefore, possible interactions of lytic bacteriophages (MS2, T4 and Phi6) with acetone and methanol S. rebaudiana extracts (SRa and SRm) in the bacterial environment were examined. Material and Methods The interactions were tested using a microdilution method, phage-extract co-incubation assay, static interaction (synography) and dynamic growth profile experiments in a bioreactor. Results The interactions of the tested factors in a static environment differed from those in a dynamic environment. Dynamic conditions altered the effect of the extracts in a concentration-dependent manner. How different the effect of the SRa extract was to that of the SRm extract on bacterial growth in a dynamic environment depended on the species of the phage and bacterial host. The greatest differences were observed for E. coli strains and their phages, whereas Pseudomonas syringae and the Phi6 phage reacted very similarly to both extracts. Differences also emerged for the same extract in different E. coli strains and their phages. Conclusion Every extract type should be tested on a case-by-case basis and experiment outcomes should not be generalised before gathering data. Moreover, many varied experiments should be performed, especially when examining such multifactorial mixtures. The tested mixtures could potentially be used in multidrug-resistant bacterial infection treatments.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135273577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterovirus E infects bovine peripheral blood mononuclear cells. Implications for pathogenesis? 肠病毒E感染牛外周血单个核细胞。对发病机制的影响?

3区农林科学

Journal of Veterinary Research Pub Date : 2023-10-24 DOI: 10.2478/jvetres-2023-0061

Joanna Małaczewska, Małgorzata Wróbel, Edyta Kaczorek-Łukowska, Wojciech Rękawek

{"title":"Enterovirus E infects bovine peripheral blood mononuclear cells. Implications for pathogenesis?","authors":"Joanna Małaczewska, Małgorzata Wróbel, Edyta Kaczorek-Łukowska, Wojciech Rękawek","doi":"10.2478/jvetres-2023-0061","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0061","url":null,"abstract":"Abstract Introduction Enterovirus E (EV-E) is a common viral pathogen endemic in cattle worldwide. Little is known, however, about its potential interactions with bovine immune cells. Material and Methods The EV-E-permissiveness of bovine peripheral blood mononuclear cells (PBMCs) was evaluated. The infectious titres of extracellular virus were measured and the intracellular viral RNA levels were determined by reverse transcription quantitative PCR after cell inoculation. The effects of EV-E on cell viability and proliferative response were investigated with a methyl thiazolyl tetrazolium bromide reduction assay, the percentages of main lymphocyte subsets and oxidative burst activity of blood phagocytes were determined with flow cytometry, and pro-inflammatory cytokine secretion was measured with an ELISA. Results Enterovirus E productively infected bovine PBMCs. The highest infectious dose of EV-E decreased cell viability and T-cell proliferation. All of the tested doses of virus inhibited the proliferation of high responding to lipopolysaccharide B cells and stimulated the secretion of interleukin 1β, interleukin 6 and tumour necrosis factor α pro-inflammatory cytokines. Conclusion Interactions of EV-E with bovine immune cells may indicate potential evasion mechanisms of the virus. There is also a risk that an infection with this virus can predispose the organism to secondary infections, especially bacterial ones.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135267457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Some aspects of the diagnosis and treatment of eosinophilic granuloma in cats 猫嗜酸性肉芽肿的诊断和治疗的一些方面

3区农林科学

Journal of Veterinary Research Pub Date : 2023-10-19 DOI: 10.2478/jvetres-2023-0060

Hanna Omelchenko, Natalia Avramenko, Serhii Kulynych, Maksym Petrenko, Vladyslav Volosovets, Natalia Volosovets, Grzegorz Woźniakowski

{"title":"Some aspects of the diagnosis and treatment of eosinophilic granuloma in cats","authors":"Hanna Omelchenko, Natalia Avramenko, Serhii Kulynych, Maksym Petrenko, Vladyslav Volosovets, Natalia Volosovets, Grzegorz Woźniakowski","doi":"10.2478/jvetres-2023-0060","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0060","url":null,"abstract":"Abstract Introduction Eosinophils represent the most active cells in mammals that show protective and assistive activity in the host immune defence against helminth parasites. These cells are also responsible for the reduction of allergic and inflammatory reactions. The eosinophils play a key role in allergic reactions by secretion of different chemical molecules leading to swelling, lesions and granuloma onset. Material and Methods The study was carried out on 30 cats with inflammatory skin lesions. The cats ranged in age from seven months to 13 years, and had an average age of three years. The research methodology included information on the disease, dermatological conclusions, concomitant disorders, medical and laboratory data and the treatment strategy. Results In total, 30 cats were diagnosed with eosinophilic granuloma complex. The distribution of lesions was 87.1% in the skin and 12.9% at the skin–mucosal junction. The lesions increased and decreased with the seasons of spring and summer, and the onset of the disease usually coincided with exposure to fleas. Conclusion Eosinophilic granuloma complex in cats is a serious pathology and frequently requires lifelong treatment, so it is important to diagnose it quickly and accurately to ensure optimal treatment of affected animals.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135778650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multidrug resistance in Haemonchus contortus in sheep - can it be overcome? 绵羊扭曲血蜱的多药耐药——能被克服吗?

3区农林科学

Journal of Veterinary Research Pub Date : 2023-10-17 DOI: 10.2478/jvetres-2023-0057

Michal Babják, Alžbeta Königová, Michaela Komáromyová, Tetiana Kuzmina, Pawel Nosal, Marián Várady

{"title":"Multidrug resistance in Haemonchus contortus in sheep - can it be overcome?","authors":"Michal Babják, Alžbeta Königová, Michaela Komáromyová, Tetiana Kuzmina, Pawel Nosal, Marián Várady","doi":"10.2478/jvetres-2023-0057","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0057","url":null,"abstract":"Abstract Introduction Gastrointestinal nematodes pose a threat to animal health and affect farmers by negatively impacting farm management. Material and Methods The study was conducted on a sheep farm with suspected reductions in the efficacies of anthelmintics. Efficacy was determined using in vivo faecal egg count reduction, in vitro egg hatch (EHT) and larval development (LDT) tests. In the first phase, 60 sheep were equally split into six groups. Group 1 received the recommended dose of albendazole (ALB), group 2 received the same after fasting for 24 h, group 3 received the dose divided into two halves at 6 h intervals, group 4 received a double dose of ALB, and group 5 received the recommended dose of ivermectin (IVM). Group 6 served as a control. The second phase of the experiment had two groups: one treated with levamisole (LEV) and a control group. Faecal samples were collected from all sheep. Results No reduction of egg output was observed in the groups treated with single, double, or divided doses of ALB, but one of 13.7–16.9% was noted in the fasting group. Efficacy in the IVM group ranged from 31.50 to 39.97%. The mean concentrations sufficient to prevent 50% of the eggs from hatching in the in vitro EHT and the mean concentrations in which the development of larvae to the L3 stage was inhibited by 50% in the LDT exceeded established thresholds for benzimidazoles and IVM. Haemonchus contortus was the only species identified after treatment. The LDT did not indicate the presence of resistance to LEV. All animals treated with LEV were negative for eggs 10 d after treatment. Conclusion Resistance to ALB and IVM in Haemonchus contortus was confirmed. Alternative approaches to improve the efficacies of benzimidazole did not sufficiently increase the efficacy, but LEV was an efficient anthelmintic treatment.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135993170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0