Journal of Veterinary Research最新文献_第8页

Chickens' eggs and the livers of farm animals as sources of perfluoroalkyl substances. 作为全氟烷基物质来源的鸡蛋和农场动物的肝脏。

IF 1.3 3区农林科学

Journal of Veterinary Research Pub Date : 2024-06-20 eCollection Date: 2024-06-01 DOI: 10.2478/jvetres-2024-0034

Szczepan Mikołajczyk, Małgorzata Warenik-Bany, Marek Pajurek

{"title":"Chickens' eggs and the livers of farm animals as sources of perfluoroalkyl substances.","authors":"Szczepan Mikołajczyk, Małgorzata Warenik-Bany, Marek Pajurek","doi":"10.2478/jvetres-2024-0034","DOIUrl":"10.2478/jvetres-2024-0034","url":null,"abstract":"Introduction: This study focuses on perfluoroalkyl substance (PFAS) content in chickens' eggs and the livers of farm animals.Material and methods: Chickens' eggs (n = 25) and the livers of cows (n = 10), chickens (n = 7) and horses (n = 3) were collected from various regions of Poland. Samples were analysed using the isotope dilution technique with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).Results: The mean lower bound (LB) sum of four PFAS (∑4 PFAS) concentrations (perfluorooctanesulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA) and perfluorohexanesulfonic acid (PFHxS)) were the highest in cows' livers (0.52 μg/kg) and much lower in chickens' (0.17 μg/kg) and horses' livers (0.13 μg/kg) and chickens' eggs (0.096 μg/kg). The ratio of ∑4 PFASs to the limits set by Commission Regulation (EU) 2023/915 was <7% for liver and <6% for eggs. Linear PFOS was the compound with the highest detection frequency (8% in eggs and 48% in all livers). In cows' livers it was detected in 80% of samples. The estimated exposure to LB ∑4 PFASs via consumption of liver tissue from farm animals (assuming 50 g and 100 g portions) was <52% of the tolerable weekly intake (TWI) for children and <17% of the TWI for adults. Dietary intake via the average portion of three eggs led to low exposure of <15% for children and <5% for adults.Conclusion: Neither eggs nor the livers of chickens or horses as analysed in this study are significant sources of PFASs, while cows' livers might contribute significantly to a child's overall dietary intake. Further investigation of PFOS in farm animal livers should be conducted.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"241-248"},"PeriodicalIF":1.3,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of doxycycline residues in bones after oral administration to broiler chickens. 调查肉鸡口服强力霉素后骨骼中的残留物。

IF 1.3 3区农林科学

Journal of Veterinary Research Pub Date : 2024-05-29 eCollection Date: 2024-06-01 DOI: 10.2478/jvetres-2024-0030

Anna Gajda, Iwona Szymanek-Bany, Ewelina Nowacka-Kozak, Małgorzata Gbylik-Sikorska

{"title":"Investigation of doxycycline residues in bones after oral administration to broiler chickens.","authors":"Anna Gajda, Iwona Szymanek-Bany, Ewelina Nowacka-Kozak, Małgorzata Gbylik-Sikorska","doi":"10.2478/jvetres-2024-0030","DOIUrl":"10.2478/jvetres-2024-0030","url":null,"abstract":"Introduction: Chicken bones, a by-product of the poultry industry, can directly or indirectly enter the food chain. Bone meal and bone products could be sources of many contaminants. Considering the wide range of uses made of bones in the culinary and food industries, this material needs to be safe and antibiotic residue-free. To determine if such is the case, the concentration of doxycycline in chicken bones was investigated, this antimicrobial being one of the most commonly used in poultry production.Material and methods: Ross 308 broilers were grouped into three experimental and one control group. Doxycycline was administered in drinking water at therapeutic and sub-therapeutic doses, as well as via spray treatment. The concentration of doxycycline in bones was determined post slaughter by ultra-high performance liquid chromatography-tandem mass spectrometry.Results: Doxycycline was quantified at 135 μg/kg 22 days after the last day of antibiotic administration at therapeutic doses; 2,285 μg/kg after sub-therapeutic treatment for 27 days and 9.62 μg/kg 22 days after the end of spray application.Conclusion: High concentrations and long persistence of doxycycline in bones were found in this study. Doxycycline can contaminate all bone-derived products in the food and fertiliser industries.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"255-261"},"PeriodicalIF":1.3,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141474659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of transfer of different sulphonamides from contaminated beeswax to honey. 比较不同磺胺类药物从受污染的蜂蜡转移到蜂蜜中的情况。

IF 1.3 3区农林科学

Journal of Veterinary Research Pub Date : 2024-05-27 eCollection Date: 2024-06-01 DOI: 10.2478/jvetres-2024-0029

Kamila Mitrowska, Maja Antczak

{"title":"Comparison of transfer of different sulphonamides from contaminated beeswax to honey.","authors":"Kamila Mitrowska, Maja Antczak","doi":"10.2478/jvetres-2024-0029","DOIUrl":"10.2478/jvetres-2024-0029","url":null,"abstract":"Introduction: No maximum residue limits in honey have been legislated in the EU for antimicrobial substances such as sulphonamides, and they are not permitted, therefore, for treating honey bees unless in a cascade system. Since sulphonamides are used illegally in apiculture to treat foulbrood, their residues can be found in honey and other apiculture products, including beeswax. The study aimed to assess the contamination of honey from beeswax containing residues of 10 sulphonamides (sulphadimethoxine (SDM), sulphadoxine (SDX), sulphamonomethoxine (SMM), sulphamethoxazole (SMX), sulphameter (SMT), sulphamethazine (SMZ), sulphamerazine (SMR), sulphadiazine (SDA), sulphathiazole (STZ) and sulphacetamide (SCA)).Material and methods: Wax-based foundations fortified with 10 sulphonamides at 10,000 μg/kg were evaluated for sulphonamide concentrations and then placed in a beehive so that honey bees (Apis mellifera L.) could build honeycombs with them. Frames of capped honey were taken out of the hives one month later and honey was sampled from them. The honeycombs were subsequently incubated in a laboratory at 35°C for five months, and honey was sampled monthly. The honey sulphonamide concentrations were measured using liquid chromatography-tandem mass spectrometry and compared to the wax-based foundation concentrations.Results: The maximum transfers to honey of the initial amount of SDM, SDX, SMM, SMX, SMT, SMZ, SMR, SDA, STZ and SCA in the wax-based foundations were 42.6, 34.3, 31.7, 30.1, 29.5, 25.2, 18.7, 16.1, 9.5 and 8.6%, respectively.Conclusion: This study demonstrated that every tested sulphonamide could migrate from beeswax in antimicrobial-tainted honeycombs to honey, SDM having the highest migration potential and SCA the lowest.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"249-254"},"PeriodicalIF":1.3,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles in cattle infected with bovine leukaemia virus: isolation and molecular analysis. 牛白血病病毒感染牛的细胞外囊泡：分离和分子分析。

IF 1.3 3区农林科学

Journal of Veterinary Research Pub Date : 2024-05-27 eCollection Date: 2024-06-01 DOI: 10.2478/jvetres-2024-0031

Maria Szczotka, Magdalena Wasiak, Jacek Kuźmak

{"title":"Extracellular vesicles in cattle infected with bovine leukaemia virus: isolation and molecular analysis.","authors":"Maria Szczotka, Magdalena Wasiak, Jacek Kuźmak","doi":"10.2478/jvetres-2024-0031","DOIUrl":"10.2478/jvetres-2024-0031","url":null,"abstract":"Introduction: Exosomes are nanosized lipid bilayer membranous microvesicles, extracellularly released from a variety of mammalian cells. They mediate intercellular signalling by transporting several types of RNA, lipids and proteins and participate in the intercellular exchange of DNA, RNA, micro RNA, proteins and other components. These microvesicles are present in all body fluids in physiological and pathological conditions and reflect the state of the host organism. The aim of the study was the isolation and molecular determination of exosomes in blood and supernatant fluids of bovine dendritic cell cultures infected with bovine leukaemia virus (BLV).Material and methods: Exosomes were isolated by ultracentrifugation from the blood sera, plasma and supernatant of bovine BLV-infected and uninfected control dendritic cell cultures and their presence was confirmed with scanning electron and transmission electron microscopy. Western blot analysis of the structural BLV glycoprotein 51 (Env) and protein 24 (Gag) and of the tetraspanin exosomal markers CD9, CD63 and flotillin-1 was undertaken in BLV+ and control BLV- cattle.Results: In exosomes of leukaemic cattle both BLV proteins and exosomal markers were detected. In healthy control animals only exosomal markers were determined.Conclusion: Proteins of BLV were released with exosomes and could be transferred into recipient cells as an alternative propagation route not requiring virus infection.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"189-198"},"PeriodicalIF":1.3,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses. 用于区分小反刍动物慢病毒 A 基因型和 B 基因型菌株的血清学和分子方法的比较。

IF 1.3 3区农林科学

Journal of Veterinary Research Pub Date : 2024-04-30 eCollection Date: 2024-06-01 DOI: 10.2478/jvetres-2024-0025

Monika Olech, Jacek Kuźmak

{"title":"Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses.","authors":"Monika Olech, Jacek Kuźmak","doi":"10.2478/jvetres-2024-0025","DOIUrl":"10.2478/jvetres-2024-0025","url":null,"abstract":"Introduction: Small ruminant lentiviruses (SRLV) cause multisystemic, degenerative and chronic disease in sheep and goats. There are five genotypes (A, B, C, D and E), of which A and B are the most widespread. The purpose of this study was to evaluate the serotyping efficiency of the Eradikit SRLV Genotyping ELISA and the molecular typing efficiency of a newly developed nested real-time PCR targeting the long terminal repeat-gag (LTR-gag) region using samples from animals infected with subtypes of SRLV known to circulate in Poland.Material and methods: A total of 97 sera samples taken from 34 sheep and 63 goats were immunoassayed, and 86 DNA samples from 31 sheep and 55 goats were tested with the PCR. All ruminants were infected with known SRLV strains of the A1, A5, A12, A13, A16, A17, A18, A23, A24, A27, B1 and B2 subtypes.Results: A total of 69 (80.2%, 95% confidence interval 71.6%-88.8%) out of 86 tested samples gave positive results in the PCR. In 17 out of the 86 (19.8%) samples, no proviral DNA of SRLV was detected. The differentiation between MVV (genotype A) and CAEV (genotype B) by PCR matched the predating phylogenetic analysis invariably. No cross-reactivity was observed. On the other hand, the proportion of samples genotyped the same by the older phylogenetic analysis and the Eradikit SRLV Genotyping ELISA was 42.3%. The test was unable to classify 40.2% of samples, and 17.5% of sera were incorrectly classified.Conclusion: Our results showed that the Eradikit SRLV genotyping kit is not a reliable method for predicting SRLV genotype, while the nested real-time PCR based on the LTR-gag region did prove to be, at least for genotypes A and B.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 2","pages":"181-188"},"PeriodicalIF":1.3,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analysis of the trypanosomatid parasite Lotmaria passim in honey bees (Apis mellifera) in Poland. 波兰蜜蜂（Apis mellifera）中锥虫寄生虫 Lotmaria passim 的系统发育分析。

IF 1.3 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0018

Maria Iller, Karolina Lipczyńska-Ilczuk, Rajmund Sokół, Grzegorz Borsuk, Agata Bancerz-Kisiel

{"title":"Phylogenetic analysis of the trypanosomatid parasite Lotmaria passim in honey bees (Apis mellifera) in Poland.","authors":"Maria Iller, Karolina Lipczyńska-Ilczuk, Rajmund Sokół, Grzegorz Borsuk, Agata Bancerz-Kisiel","doi":"10.2478/jvetres-2024-0018","DOIUrl":"10.2478/jvetres-2024-0018","url":null,"abstract":"Introduction: Lotmaria passim (L. passim) is a single-celled flagellate which colonises the bee gastrointestinal tract and is highly prevalent in honey bees. This parasite is associated with colony losses. Honey bee (Apis mellifera) colonies were sampled from five apiaries in the north-eastern part of Poland for the phylogenetic analysis of L. passim.Material and methods: Each apiary consisted of approximately 60 bee colonies, of which 20 were randomly selected. Samples of 60 differently aged worker bees were collected from each colony and pooled. A total of 100 bee colonies from five apiaries were examined. Protozoa of the Trypanosomatidae family were identified by PCR. L. passim was detected in 47 (47%) of the samples. The 18S ribosomal (r) RNA amplicons of L. passim were sequenced by a commercial service. Their sequences were analysed with BLASTN and noted to be compatible with the GenBank sequences of this region of the organism's genome. A sequence analysis was performed using the BioEdit Sequence Alignment Editor and Clustal W software.Results: The amplicon sequences of L. passim were 100% homologous with the sequences deposited in GenBank under accession numbers KM066243.1., KJ684964.1 and KM980181.1.Conclusion: This is the first study to perform a phylogenetic analysis of L. passim in Polish honey bees. The analysis demonstrated high levels of genetic similarity between isolates of L. passim colonising apiaries in the north-eastern region of Poland.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 1","pages":"123-127"},"PeriodicalIF":1.3,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first confirmed cases of pigeon rotavirus A (RVA) infection in domestic pigeons (Columba livia) in Poland. 波兰首例家鸽轮状病毒 A (RVA) 感染确诊病例。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0006

Krzysztof Adamczyk, Dennis Rubbenstroth, Aleksandra Ledwoń, Rafał Sapierzyński, Piotr Szeleszczuk

{"title":"The first confirmed cases of pigeon rotavirus A (RVA) infection in domestic pigeons (Columba livia) in Poland.","authors":"Krzysztof Adamczyk, Dennis Rubbenstroth, Aleksandra Ledwoń, Rafał Sapierzyński, Piotr Szeleszczuk","doi":"10.2478/jvetres-2024-0006","DOIUrl":"10.2478/jvetres-2024-0006","url":null,"abstract":"Introduction: Although the presence of rotaviruses in pigeon samples has been reported since the 1980s, its importance as an aetiological agent of the \"classical\" young pigeon disease (YPD) was not proven until 2020, when the Henle-Koch postulates were confirmed for pigeon-type rotavirus A (RVA) genotype G18P(17).Material and methods: From 2011 to 2020, archived liver samples from 117 pigeons submitted by 74 individual lofts were tested for the presence of pigeon-type RVA using a VP6-specific RT-qPCR test. For four positive racing pigeons, a more detailed necropsy and histopathological analysis was performed.Results: Indicators of an acute RVA infection were found in 24 out of 117 (20.5%) samples tested, the earliest in 2014. Necropsies of the four selected RVA-positive pigeons showed changes mainly in the liver, spleen and kidneys similar to those described by other researchers. The histopathological examination revealed mainly hyperaemia and necrosis in the liver, as well as mononuclear cell infiltrates in these organs.Conclusion: Pigeon-type RVA is also a cause of YPD in Poland and is a serious challenge for racing pigeon breeders and veterinarians, especially during the training and flights of young pigeons.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 1","pages":"55-61"},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue expression of porcine transient receptor potential mucolipin protein channels and their differential responses to porcine reproductive and respiratory syndrome virus infection in vitro. 猪瞬时受体电位粘脂蛋白通道的组织表达及其对猪繁殖与呼吸综合征病毒体外感染的不同反应。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0014

Zhiqiang Xia, Denggao Long, Xinyi Hong, Ying Lan, Lixia Xie

{"title":"Tissue expression of porcine transient receptor potential mucolipin protein channels and their differential responses to porcine reproductive and respiratory syndrome virus infection in vitro.","authors":"Zhiqiang Xia, Denggao Long, Xinyi Hong, Ying Lan, Lixia Xie","doi":"10.2478/jvetres-2024-0014","DOIUrl":"10.2478/jvetres-2024-0014","url":null,"abstract":"Introduction: Porcine reproductive and respiratory syndrome virus (PRRSV) infection results in a serious disease, posing a huge economic threat to the global swine industry. The transient receptor potential mucolipin proteins (TRPMLs) have been shown to be strongly associated with virus infection and other physiological processes in humans, but their tissue distribution and responses to PRRSV in pigs remain unknown.Material and methods: Quantitative reverse-transcription PCR analysis was undertaken to determine the optimal primer for TRPML expression detection and for quantifying that expression individually in different pig tissue samples. Meat Animal Research Center 145 (MARC-145) monkey kidney cells and the TRPML-specific activator mucolipin synthetic agonist 1 (ML-SA1) were used to reveal the relationship between TRPML and PRRSV-2 infection.Results: The best primers for each TRPML gene used in a fluorescence-based quantitative method were identified and TRPML1 was found to be highly expressed in the kidneys and liver of pigs, while TRPML2 and TRPML3 were observed to be primarily expressed in the kidney and spleen tissues. The expression of TRPML2 was upregulated with the rise in PRRSV-2 infection titre but not the expression of TRPML1 or TRPML3, and ML-SA1 inhibited PRRSV-2 in a dose-dependent manner.Conclusion: Our research revealed the gene expression of TRPMLs in pigs and identified that TRPML channels may act as key host factors against PRRSV infection, which could lead to new targets for the prevention and treatment of pig infectious diseases.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 1","pages":"45-53"},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrine and icariin can inhibit bovine viral diarrhoea virus replication by promoting type I interferon response in vitro. 马钱子碱和冰片苷可通过促进体外 I 型干扰素反应来抑制牛病毒性腹泻病毒的复制。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0013

Dongjie Cai, Zifan Shen, Bin Tian, Jie Chen, Yilin Zhang, Liuhong Shen, Ya Wang, Xiaoping Ma, Zhicai Zuo

{"title":"Matrine and icariin can inhibit bovine viral diarrhoea virus replication by promoting type I interferon response in vitro.","authors":"Dongjie Cai, Zifan Shen, Bin Tian, Jie Chen, Yilin Zhang, Liuhong Shen, Ya Wang, Xiaoping Ma, Zhicai Zuo","doi":"10.2478/jvetres-2024-0013","DOIUrl":"10.2478/jvetres-2024-0013","url":null,"abstract":"Introduction: Bovine viral diarrhoea virus (BVDV) can cause diarrhoea (BVD) in an animal herd, leading to heavy economic losses. There are limited drugs available for treating and controlling BVD. This research aims to investigate the antiviral and immunoregulatory effects of two traditional Chinese herb extracts against BVDV infection. The extracts are matrine and icariin, which have been proved to have immunostimulant and antiviral effects.Material and methods: A cell counting kit-8 assay was used to analyse the toxicity of matrine and icariin to Madin-Darby bovine kidney (MDBK) cells. The model of MDBK cells infected with BVDV was utilised to uncover the antiviral mechanism of matrine and icariin, which along with their immunoregulatory ability was evaluated by quantitative reverse-transcription PCR and ELISA.Results: The results showed that matrine and icariin can significantly inhibit the gene expression level of the BVDV 5' untranslated region through various pathways. Both matrine and icariin can statistically upregulate the gene expression level of interferon alpha, interferon beta (IFN-β), toll-like receptor 3, retinoic acid-inducible gene I and interferon regulatory factor 3, and raise the concentration of IFN-β after BVDV infection.Conclusion: This study proves that both matrine and icariin have inhibitory effects on BVDV replication by activating IFN production and the IFN signalling pathway. The finding is promising and should open up the possibility of larger-scale in vitro research followed by in vivo experiments evaluating matrine and icariin as therapeutic agents in BVD cases.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 1","pages":"35-44"},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon. 富血小板和贫血小板浓缩物刺激后单核细胞衍生巨噬细胞的活性。对掺硅类金刚石碳涂层胫骨植入物插入绵羊模型的研究。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0003

Bartłomiej Szymczak, Andrzej Junkuszew, Krzysztof Patkowski, Tomasz Szponder, Dominika Nguyen Ngoc, Beata Drzewiecka, Aleksandra Sobczyńska-Rak, Joanna Wessely-Szponder

{"title":"The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon.","authors":"Bartłomiej Szymczak, Andrzej Junkuszew, Krzysztof Patkowski, Tomasz Szponder, Dominika Nguyen Ngoc, Beata Drzewiecka, Aleksandra Sobczyńska-Rak, Joanna Wessely-Szponder","doi":"10.2478/jvetres-2024-0003","DOIUrl":"10.2478/jvetres-2024-0003","url":null,"abstract":"Introduction: Macrophages are crucial immune cells that play a role in tissue repair and can exhibit pro- or anti-inflammatory behaviour based on environmental stimulation. Their functional phenotype can be affected by platelet-derived products as determined by those products' composition. When the inflammatory response caused by implantation is excessive, it can lead to rejection of the implant. Therefore, a thorough evaluation of implant haemocompatibility is necessary to minimise undesirable consequences.Material and methods: In an in vitro study, monocyte-derived macrophages (MDMs) were obtained from the whole blood of sheep after a silicon-doped diamond-like carbon-coated implant insertion. These MDMs were then exposed to autologous platelet-derived products for functional marker analysis.Results: Platelet-poor plasma (PPP) and pure platelet-rich plasma (P-PRP) stimulation increased arginase-1 activity, while leukocyte-rich PRP stimulation produced a mixed response involving higher O2- (6.49 ± 2.43 nM vs non-stimulated 3.51 ± 1.23 nM, P-value < 0.05) and NO (3.28 ± 1.38 μM vs non-stimulated 2.55 ± 0.32μM, P-value < 0.05) generation.Conclusion: Using PPP and P-PRP stimulation in post-implantation procedures may contribute to the polarisation of macrophages towards the M2-like pro-resolving phenotype, thereby accelerating wound healing. This would also prevent implant degradation due to an excessive inflammatory process.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 1","pages":"167-174"},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0