Journal of Veterinary Research最新文献

{"title":"Prevalence of <i>Borrelia burgdorferi</i> and <i>Anaplasma phagocytophilum</i> in <i>Ixodes ricinus</i> collected from dogs in eastern Poland.","authors":"Anna Pańczuk, Małgorzata Tokarska-Rodak, Patrycja Andrzejuk","doi":"10.2478/jvetres-2024-0015","DOIUrl":"10.2478/jvetres-2024-0015","url":null,"abstract":"<p><strong>Introduction: </strong><i>Ixodes ricinus</i> ticks are an important vector and reservoir of pathogenic microorganisms causing dangerous infectious diseases in humans and animals. The presence of ticks in urban greenery is a particularly important public health concern due to the potential for humans and companion animals to be exposed to tick-borne diseases there. The study assessed the prevalence of <i>Borrelia burgdorferi</i> and <i>Anaplasma phagocytophilum</i> infection in <i>I. ricinus</i> ticks feeding on dogs.</p><p><strong>Material and methods: </strong>The study consisted in analyses of <i>I. ricinus</i> ticks collected in 2018-2020 from owned and stray dogs in the north-eastern part of Lubelskie province (eastern Poland). An AmpliSens PCR kit was used for qualitative detection and differentiation of tick-borne infections.</p><p><strong>Results: </strong>Infections of <i>B. burgdorferi</i> and <i>A. phagocytophilum</i> were detected in 10.9% and 12.9% of the examined ticks, respectively. One tick (0.7%) was co-infected by both pathogens. Infection with <i>B. burgdorferi</i> was significantly more highly prevalent in ticks collected from the owned dogs than from the strays (18.7% and 2.8%, respectively), whereas the prevalence of <i>A. phagocytophilum</i> was similar in both groups (12.0% and 13.9%, respectively).</p><p><strong>Conclusion: </strong>The co-infection observed in the study suggests the possibility of simultaneous infection by both pathogens from a single tick bite. The presence of pathogens in ticks collected from dogs is a factor in assessing infection risk not only to companion animals but also to their owners, who are in close contact with their dogs and visit the same green areas recreationally.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 1","pages":"109-114"},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development, in-house validation and application of a method using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) for the quantification of 12 ergot alkaloids in compound feeds.","authors":"Ewelina Kowalczyk, Krzysztof Kwiatek","doi":"10.2478/jvetres-2023-0070","DOIUrl":"10.2478/jvetres-2023-0070","url":null,"abstract":"<p><strong>Introduction: </strong>Ergot alkaloids (EAs) are toxic substances naturally produced by <i>Claviceps</i> fungi. These fungi infest a wide range of cereals and grasses. When domestic animals are exposed to EAs through contaminated feeds, it is detrimental to them and leads to significant economic losses. For that reason, it is important to monitor feed for the presence of EAs, especially with methods enabling their determination in processed materials.</p><p><strong>Material and methods: </strong>Ergot alkaloids were extracted with acetonitrile, and dispersive solid phase extraction (d-SPE) was used for clean-up of the extracts. After evaporation, the extracts were reconstituted in ammonium carbonate and acetonitrile and subjected to instrumental analysis using high-performance liquid chromatography with fluorescence detection. The developed method was validated in terms of linearity, selectivity, repeatability, reproducibility, robustness, matrix effect, limits of quantification and detection and uncertainty. The EA content of 40 compound feeds was determined.</p><p><strong>Results: </strong>All the assessed validation parameters fulfilled the requirements of Regulation (EU) 2021/808. At least one of the monitored alkaloids was determined in 40% of the samples. The EAs with the highest incidence rate were ergocryptine, ergometrinine and ergocornine. The total concentrations of EAs ranged from under the limit of quantification to 62.3 μg kg^-1.</p><p><strong>Conclusion: </strong>The results demonstrated that the developed method was suitable for simultaneously determining twelve EAs in compound feed and could be used for routine analysis.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"67 4","pages":"603-610"},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138835939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The serological and genetic diversity of the <i>Leptospira interrogans</i> Icterohaemorrhagiae serogroup circulating in the UK.","authors":"Zbigniew Arent, Colm Gilmore, Laura Pardyak, Klaudia Dubniewicz, Barry McInerney, William Ellis","doi":"10.2478/jvetres-2023-0063","DOIUrl":"10.2478/jvetres-2023-0063","url":null,"abstract":"<p><strong>Introduction: </strong>Strains of <i>Leptospira interrogans</i> belonging to two very closely related serovars, Icterohaemorrhagiae and Copenhageni, have been associated with disease in mammalian species and are the most frequently reported agents of human leptospirosis. They are considered the most pathogenic serovars and represent more than half of the leptospires encountered in severe human infections.</p><p><strong>Material and methods: </strong>Nineteen such isolates from the United Kingdom - human, domestic and wildlife species - were typed using three monoclonal antibodies (F12 C3, F70 C14 and F70 C24) in an attempt to elucidate their epidemiology. They were further examined by restriction endonuclease analysis (REA), multiple-locus variable-number tandem repeat analysis (MLVA) and lic12008 gene sequence analysis.</p><p><strong>Results: </strong>Monoclonal antibody F12 C3, which is highly specific for Icterohaemorrhagiae and Copenhageni, confirmed that all the strains belonged to these two serovars. Sixteen strains were identified as Copenhageni and three as Icterohaemorrhagiae serovar. Only one restriction pattern type was identified, thus confirming that REA is not able to discriminate between the Icterohaemorrhagiae and Copenhageni serovars. Variable-number tandem-repeat analysis found three loci with differences in the repeat number, indicating genetic diversity between British isolates. Sequences of the lic12008 gene showed that all isolates identified as the Icterohaemorrhagiae serotype have a single base insertion, in contrast to the same sequences of the Copenhageni serotype.</p><p><strong>Conclusion: </strong>Copenhageni is the predominant serovar in the Icterohaemorrhagiae serogroup isolated in British Isles. There is a genetic diversity of MLVA patterns of the isolates but no genetic tool used in the study was able to determine serovars.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"67 4","pages":"529-536"},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genetic variability of small-ruminant lentiviruses and its impact on tropism, the development of diagnostic tests and vaccines and the effectiveness of control programmes.","authors":"Monika Olech","doi":"10.2478/jvetres-2023-0064","DOIUrl":"10.2478/jvetres-2023-0064","url":null,"abstract":"<p><strong>Introduction: </strong>Maedi-visna virus and caprine arthritis encephalitis virus are two closely related lentiviruses which cause multisystemic, progressive and persistent infection in goats and sheep. Because these viruses frequently cross the species barrier, they are considered to be one genetic group called small-ruminant lentiviruses (SRLV). They have <i>in vivo</i> tropism mainly for monocytes and macrophages and organ tropism with unknown mechanisms. Typical clinical signs are pneumonia in sheep, arthritis in goats, and mastitis in both species. Infection with SRLV cannot currently be treated or prevented, and control programmes are the only approaches to avoiding its spread. These programmes rely mainly on annual serological testing and elimination of positive animals. However, the high genetic and antigenic variability of SRLV complicate their early and definitive diagnosis. The objective of this review is to summarise the current knowledge of SRLV genetic variation and its implications for tropism, the development of diagnostic tests and vaccines and the effectiveness of control and eradication programmes.</p><p><strong>Material and methods: </strong>Subject literature was selected from the PubMed and the Google Scholar databases.</p><p><strong>Results: </strong>The high genetic diversity of SRLV affects the performance of diagnostic tools and therefore control programmes. For the early and definitive diagnosis of SRLV infection, a combination of serological and molecular tests is suggested. Testing by PCR can also be considered for sub-yearling animals. There are still significant gaps in our knowledge of the epidemiology, immunology and biology of SRLV and their impact on animal production and welfare.</p><p><strong>Conclusion: </strong>This information may aid selection of the most effective SRLV spread reduction measures.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"67 4","pages":"479-502"},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular contamination of an animal facility during and after African swine fever virus infection.","authors":"Marek Walczak, Krzesimir Szymankiewicz, Fernando Rodriguez, Jordi Argilaguet, Boris Gavrilov, Jacek Żmudzki, Maciej Kochanowski, Małgorzata Juszkiewicz, Anna Szczotka-Bochniarz","doi":"10.2478/jvetres-2023-0065","DOIUrl":"10.2478/jvetres-2023-0065","url":null,"abstract":"<p><strong>Introduction: </strong>The molecular contamination of an animal facility was investigated during and after an infection with highly pathogenic African swine fever virus (ASFV) among domestic pigs. The investigation evaluated the risk of indirect transmission of the disease and indicated points that may facilitate cleaning and disinfection processes.</p><p><strong>Material and methods: </strong>Six domestic pigs were infected oronasally with the highly pathogenic Georgia 2007 strain. Environmental samples from the floors, walls, rubber floor mats, feeders, drinkers, high-efficiency particulate-absorbing filter covers and doors were collected 7 days post infection (dpi), 7 days later and 24 h after disinfection of the facility. The samples were investigated by real-time PCR and <i>in vitro</i> assays to find genetic traces of ASFV and infectious virus.</p><p><strong>Results: </strong>Typical clinical outcomes for ASF (<i>i.e</i>. fever, apathy, recumbency and bloody diarrhoea) were observed, and all animals died or required euthanasia before or at 9 dpi. No infectious virus was found in environmental samples at the sampling time points. Genetic traces of ASFV were found in all locations except the doors. The initial virus load was calculated using real-time PCR threshold cycle values and was the highest at the drain. A statistically significant decrease of virus load over time was found on non-porous surfaces mechanically cleaned by water (the floor and drain).</p><p><strong>Conclusion: </strong>The gathered data confirmed different routes of virus excretion (oral and nasal, faeces and urine, and aerosol) and showed virus locations and different initial concentrations in the animal facility. Maintaining the facility with mechanical cleaning and using personal protection (gloves) and hand disinfection may efficiently minimise the risk of further virus spread. Together with the results of previously published studies, the present investigations' failure to isolate infectious virus may suggest that if stable environmental conditions are assured, the time needed before the introduction of new herds into previously ASF-affected farm facilities could be shortened and in this way the economic losses caused by the disease outbreak mitigated.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"67 4","pages":"503-508"},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6).","authors":"Piotr Kubiś, Jacek Kuźmak","doi":"10.2478/jvetres-2023-0069","DOIUrl":"10.2478/jvetres-2023-0069","url":null,"abstract":"<p><strong>Introduction: </strong>Bovine herpesvirus 6 (BoHV6) belongs to the <i>Herpesviridae</i> family, <i>Gammaherpesvirinae</i> subfamily and <i>Macavirus</i> genus. It is common in cattle, but was also detected in American bison <i>(Bison bison)</i> and water buffalo <i>(Bubalus bubalis</i>). The aim of the experiment was to develop an ELISA for serological examination of cattle sera for the presence of anti-BoHV6 specific antibodies.</p><p><strong>Material and methods: </strong>Viral DNA from a BoHV6-positive cow was amplified by qPCR and the resulting fragments of the <i>gB</i> and <i>gH</i> genes encoding glycoproteins B and H (gB and gH) were cloned into the pLATE52 vector to express recombinant gB (rgB) and gH (rgH) in Rosetta (DE3) <i>E. coli</i>. The expressed recombinant proteins were used as antigens in the developed ELISA.</p><p><strong>Results: </strong>The proteins expressed had the expected molecular weight. A total of 143 sera were examined, and 141 of them were positive, according to the chosen cut-off values of 9% and 10% for the sample-to-positive ratios of the rgB and rgH antigens, respectively.</p><p><strong>Conclusion: </strong>The rgB and rgH recombinant antigens of BoHV6 were successfully expressed in <i>E. coli</i> and successfully used in a newly developed ELISA.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"67 4","pages":"509-515"},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoreactivity of p21, MMP-1 and CB2 receptor proteins in cutaneous canine mast cell tumours: an association with the three-tier grading system.","authors":"Kamila Bulak, Anna Kycko, Anna Śmiech, Wojciech Łopuszyński","doi":"10.2478/jvetres-2023-0066","DOIUrl":"10.2478/jvetres-2023-0066","url":null,"abstract":"<p><strong>Introduction: </strong>Mast cell tumours (MCTs) arise in the dermis and subcutaneous tissues in animals and humans and are one of the most common neoplasms of the skin in dogs. Cannabinoid type 2 receptor (CB2R), cyclin-dependent kinase inhibitor (p21) and matrix metalloproteinase 1 (MMP-1) are potential targets for novel anti-tumour therapeutic strategies. This study evaluated by immunohistochemical means the reactivity of p21, MMP-1 and CB2R proteins in association with a three-tier grading system in cutaneous canine MCTs.</p><p><strong>Material and methods: </strong>Formalin-fixed, paraffin-embedded canine MCTs were processed for histochemical analysis and immunohistochemical staining using antibodies against p21, MMP-1 and CB2R. The results were analysed statistically.</p><p><strong>Results: </strong>The strongest p21 immunolabelling was detected in grade 3 MCTs, while grade 1 tumours showed mild or no detectable p21 immunoreactivity (P-value < 0.001). Strong immunolabelling of MMP-1 was the most common in grade 1 tumours (P-value < 0.001) and CB2R was significantly less frequent in grade 3 tumours than in grade 1 (P-value < 0.001) and grade 2 (P-value < 0.001).</p><p><strong>Conclusion: </strong>High immunoreactivity of MMP-1 can be a marker of grade 1 MCTs in dogs, whereas p21 protein overexpression can be a marker of grade 3 canine MCTs. Strong CB2R immunoreactivity with simultaneous underexpression of p21 and high immunoreactivity of MMP-1 proteins may indicate that the use of cannabinoids in grade 1 MCTs in dogs is practicable.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"67 4","pages":"611-618"},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why does Listeria monocytogenes survive in food and food-production environments?","authors":"J. Osek, K. Wieczorek","doi":"10.2478/jvetres-2023-0068","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0068","url":null,"abstract":"Abstract Listeria monocytogenes is one of the most dangerous food-borne pathogens and is responsible for human listeriosis, a severe disease with a high mortality rate, especially among the elderly, pregnant women and newborns. Therefore, this bacterium has an important impact on food safety and public health. It is able to survive and even grow in a temperature range from -0.4°C to 45°C, a broad pH range from 4.6 to 9.5 and at a relatively low water activity (aW < 0.90), and tolerates salt content up to 20%. It is also resistant to ultraviolet light, biocides and heavy metals and forms biofilm structures on a variety of surfaces in food-production environments. These features make it difficult to remove and allow it to persist for a long time, increasing the risk of contamination of food-production facilities and ultimately of food. In the present review, the key mechanisms of the pathogen’s survival and stress adaptation have been presented. This information may grant better understanding of bacterial adaptation to food environmental conditions.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"117 26","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138607401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insight on chlamydiae","authors":"M. Szymańska-Czerwińska, Kinga Zaręba-Marchewka, K. Niemczuk","doi":"10.2478/jvetres-2023-0067","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0067","url":null,"abstract":"Abstract This article provides an overview of the current knowledge on chlamydiae, which are intracellular bacteria belonging to the Chlamydiaceae family. Whole-genome sequencing leads to great increases in the available data about Chlamydia spp. Recently, novel chlamydial taxons in various hosts living in different environments have been recognised. New species and taxons with Candidatus status have been recorded mainly in birds and reptiles. Chlamydia gallinacea is an emerging infectious agent in poultry with indirectly confirmed zoonotic potential. Recently, a new group of avian C. abortus strains with worldwide distribution in various wild bird families has been described. The definition of C. abortus species became outdated with the discovery of these strains and has been amended. It now includes two subgroups, mammalian and avian, the latter including all isolates hitherto referred to as atypical C. psittaci or C. psittaci/C. abortus intermediates.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"65 12","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138606536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland","authors":"Sebastian Smulski, Marcin Pszczoła, Monika Stachowiak, Adrianna Bilińska, Izabela Szczerbal","doi":"10.2478/jvetres-2023-0062","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0062","url":null,"abstract":"Abstract Introduction MicroRNAs (miRNAs), a class of noncoding small RNAs, have been recognised as potential biomarkers of mammary gland conditions, including bovine mastitis diagnosis. The aim of this study was to quantify selected miRNAs in the milk of mastitic cows. Material and Methods Milk samples (n = 90) were collected from healthy and mastitic dairy cows originating from local dairy cattle farms located in the west of Poland. MicroRNAs of the miR-21a, miR-92a, miR-146a and miR-383 species were quantified using the highly sensitive droplet digital PCR method. Direct measurement of somatic cell count (SCC) was performed using a cell counter. Cows were divided into three groups: those with an SCC below 200,000/mL were designated Low (n = 25), those with an SCC between 200,000 and 999,999 were Medium (n = 34), and those with an SCC of 1,000,000 or higher were High (n = 31). Microbiological analyses were performed using standard culture testing. Results The level of miR-383 was very low and this miRNA was excluded from analysis. The miR-92a was used to normalise miR-21a and miR-146a expression levels. The obtained results of expression of miR-21a and miR-146a correlated with somatic cell number (R = 0.53 and 0.79, respectively). Conclusion These results show that ddPCR is a useful method for quantifying miRNAs in raw cow milk. It seems that miR-146a is a promising marker for bovine mastitis, although further studies are needed to select a panel of miRNAs that can be used in mastitis monitoring in Poland.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135342246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}