Journal of Veterinary Research最新文献

{"title":"First molecular detection of <i>Brachyspira suanatina</i> on pig farms in Poland.","authors":"Piotr Cybulski,&nbsp;Katrin Strutzberg-Minder,&nbsp;Edyta Michalik,&nbsp;Radosław Kondratiuk,&nbsp;Artur Jablonski","doi":"10.2478/jvetres-2023-0038","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0038","url":null,"abstract":"<p><strong>Introduction: </strong>Prior to the 2000s, swine dysentery was considered to be caused only by <i>Brachyspira hyodysenteriae</i> with contributing commensal intestinal anaerobes. Nowadays, it is known that the disease is caused by three strongly beta-haemolytic species of the anaerobic spirochaetal genus <i>Brachyspira, i.e. B. hyodysenteriae</i> and newly emerged <i>B. hampsonii</i> and <i>B. suanatina.</i></p><p><strong>Material and methods: </strong>The present investigation was carried out in November 2022 on nine Polish high-performing finisher pig farms. At every location one fresh pooled faecal sample was collected from 40 randomly selected pigs of between 60 and 110 kg live weight. Nucleic acid extracted from each pooled faecal sample was analysed by an in-house multiplex PCR for <i>Brachyspira</i> spp<i>.</i>, which is capable of confirming the <i>Brachyspira</i> genus and detecting and differentiating <i>Brachyspira</i> species.</p><p><strong>Results: </strong>From a total of nine samples examined, the genetic material of <i>B. suanatina</i> was detected in seven. Non-pathogenic/questionably pathogenic <i>Brachyspira</i> spp. were found in six samples.</p><p><strong>Conclusion: </strong>To the best of our knowledge, this is the first report on the identification of <i>B. suanatina</i> in pigs outside Scandinavia, Germany and the United Kingdom. Our research not only provides valuable epidemiological data on <i>B. suanatina</i> infection in Europe but also highlights both the importance of modern laboratory diagnostics and the need for thorough investigation across regions, including retrospective studies.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/72/57/jvetres-67-3-jvetres-2023-0038.PMC10541668.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilisation of Actiphage in combination with IS<i>900</i> qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds.","authors":"Monika Beinhauerova,&nbsp;Iva Slana","doi":"10.2478/jvetres-2023-0041","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0041","url":null,"abstract":"<p><strong>Introduction: </strong><i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was to evaluate the possibility of rapid detection of viable MAP on small ruminant farms based on environmental sample examination using a novel phage-based test named Actiphage.</p><p><strong>Material and methods: </strong>A total of 9 fresh and 28 frozen (8 or 11 years at -70°C) environmental samples originating from paratuberculosis-affected farms were analysed for the presence of MAP by four different diagnostic methods: Actiphage combined with real-time PCR targeting insertion sequence <i>900</i> (IS<i>900</i> qPCR), conventional phage amplification assay, culture (frozen samples only), and direct <i>ĪS900</i> qPCR.</p><p><strong>Results: </strong>Viable MAP was detected in one fresh environmental sample using Actiphage-IS<i>900</i> qPCR. None of the frozen samples tested positive using this diagnostic approach, which was consistent with the results of culture examination also providing information on viability.</p><p><strong>Conclusion: </strong>This study describes other possible and innovative uses of phage-based methods in paratuberculosis control strategies. The Actiphage-qPCR was found to be less laborious than culture and provided results within six hours, suggesting that it may be a valuable tool for rapid initial determination of the infectious status of farmed animals based on environmental sample examination.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d5/f7/jvetres-67-3-jvetres-2023-0041.PMC10541669.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examination of the haematological profile of pregnant Polish Holstein-Friesian black-and-white cattle in the early stage.","authors":"Marcjanna Wrzecińska,&nbsp;Alicja Kowalczyk,&nbsp;Ewa Czerniawska-Piątkowska,&nbsp;Władysław Kordan,&nbsp;Jose Pedro Araujo","doi":"10.2478/jvetres-2023-0043","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0043","url":null,"abstract":"<p><strong>Introduction: </strong>Cattle health and welfare are monitored <i>via</i> the analysis of the haematological profile, and it shows cattle's ability to adapt to changing environmental conditions, pregnancy and lactation; profile changes also indicate reproductive disorders. The literature lacks reports of the examination of the haematological profile in cows up to the 50^th day of pregnancy (dop). Therefore, this research examined that in cows up to this pregnancy stage.</p><p><strong>Material and methods: </strong>A total of 101 Polish Holstein-Friesian black-and-white cows were divided into groups. The control groups consisted of non-pregnant heifers (group C00) and non-pregnant cows (group C0), and the experimental groups were pregnant heifers (group T1 at dop ≤ 28 and group T2 at dop ≥ 29-dop < 45) and pregnant cows (group T3 at dop ≥ 29-dop ≤ 50). In addition, the T3 group was divided into cows pregnant for up 45 dop and cows between 45 and 50 dop. Blood samples were collected in March and April 2021 from each animal and analysed. A transrectal ultrasound examination was performed to detect and confirm pregnancy.</p><p><strong>Results: </strong>Statistically significant differences (P ≤ 0.01) between the group of cows at dop < 45 dop and those at dop ≥ 45-dop ≤ 50 dop were noted in granulocyte percentage (GRA%), white and red blood cell counts (WBC/RBC), platelets (PLT), platelet distribution width (PDW), haematocrit (HCT) and lymphocyte percentage (LYM%). No statistically significant differences were found in the mean corpuscular haemoglobin, monocytes (MON), monocyte percentage (MON%), mean platelet volume (MPV), thrombocrit or red blood cell distribution width (RDW). Similar statistically significant differences (P ≤ 0.01) emerged between the groups of heifers in PLT, GRA, RBC, lymphocytes, LYM% and HCT, and no significant differences were found between MPV, MON, MON% or RDW.</p><p><strong>Conclusion: </strong>Examining the haematological profile in high-yielding cattle is vital in maintaining herd profitability and high reproduction, which depend on the quick diagnosis of disorders facilitated by haematology. This study analysed the haematology profile of dairy cattle at dop ≤ 50 for the first time, indicating changes in lymphocyte levels, which suggests that the animals experienced direct stress during the study.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/32/02/jvetres-67-3-jvetres-2023-0043.PMC10541671.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41135377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possibilities of using mussels (<i>Mytilus galloprovincialis</i>) to predict rotavirus contamination in Albania.","authors":"Arta Lugaj,&nbsp;Kastriot Korro,&nbsp;Anna Maria Tolomeo,&nbsp;Rigers Bakiu,&nbsp;Gianfranco Santovito","doi":"10.2478/jvetres-2023-0053","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0053","url":null,"abstract":"<p><strong>Introduction: </strong>Rotaviruses are non-enveloped viruses that each consist of 11 double-stranded RNA molecules. These viruses are able to persist in the environment, and therefore play a fundamental role in the epidemiology of gastroenteritis and severe diarrhoea in children worldwide. While mussels have been primarily used as indicators of chemical pollution, they can also be used to monitor viral contamination. The purpose of this study was to demonstrate that the <i>Mytilus galloprovincialis</i> mussel can also be used to detect microbial contamination, owing to its tendency to naturally concentrate viruses and other pathogens.</p><p><strong>Material and methods: </strong>A total of 102 <i>Mytilus galloprovincialis</i> mussel samples from Albania were collected over a three-year period: 37 samples off the Cape of Stillo in 2015, 39 samples from Butrinti Lake in 2019 and 26 samples from Butrinti Lake in 2021.</p><p><strong>Results: </strong>The presence of rotavirus in the Cape of Stillo samples in 2015 was noted in 47% of samples from site 1, 33% from site 2, and 52% from site 3. In Butrinti Lake the percentage of infected individuals in 2019 was 33% from site 1, 41% from site 2, and 33% from site 3, whereas in 2021, it was 50% from site 1, 19% from site 2, and 0% from site 3. In total the percentage of infected individuals off the Cape of Stillo in 2015 was 44%, in Butrinti Lake in 2019 it was 36%, and in Butrinti Lake in 2021 it was 23 %.</p><p><strong>Conclusion: </strong>These results indicate the presence of rotavirus in the shellfish specimens tested, and further analysis is needed to assess the potential health risks associated with consuming these shellfish. This study also indicates that mussels can be used in marine virological surveillance programmes.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/16/7d/jvetres-67-3-jvetres-2023-0053.PMC10541656.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41125708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inter-laboratory validation of liquid chromatography-tandem mass spectrometry multi-mycotoxin determination in animal feed - method transfer from the reference laboratory to regional laboratories.","authors":"Piotr Jedziniak,&nbsp;Łukasz Panasiuk,&nbsp;Katarzyna Pietruszka,&nbsp;Krzysztof Kolano,&nbsp;Radosław Zbytniewski","doi":"10.2478/jvetres-2023-0045","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0045","url":null,"abstract":"<p><strong>Introduction: </strong>The results are presented of the inter-laboratory validation of a liquid chromatography-tandem mass spectrometry method for the determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1, fumonisin B2, ochratoxin A, toxin T-2, toxin HT-2 and zearalenone) in animal feeds.</p><p><strong>Material and methods: </strong>This study was an essential part of the method's transfer from the National Reference Laboratory to six regional laboratories in Poland working in the official survey of mycotoxins in feed. The laboratories received a batch of standard solutions, blank samples and quality control materials on which to perform analysis with one procedure and different liquid chromatography-tandem mass spectrometry conditions.</p><p><strong>Results: </strong>The validation results show good precision (reproducibility coefficient of variation 3.7-20.5%) and accuracy of the method (recovery 89-120% and trueness 94-103%) and sufficient skills of the laboratory personnel.</p><p><strong>Conclusion: </strong>The study is an example of the successful transfer of the method among laboratories.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e9/a6/jvetres-67-3-jvetres-2023-0045.PMC10541672.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41149325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of <i>bla</i>TEM and <i>bla</i>CTX-M genes in multidrug-resistant <i>Escherichia coli</i> found in milk samples from dairy cattle farms in Tulungagung, Indonesia.","authors":"Agus Widodo,&nbsp;Mirni Lamid,&nbsp;Mustofa Helmi Effendi,&nbsp;Wiwiek Tyasningsih,&nbsp;Dadik Raharjo,&nbsp;Aswin Rafif Khairullah,&nbsp;Shendy Canadya Kurniawan,&nbsp;Lita Rakhma Yustinasari,&nbsp;Katty Hendriana Priscilia Riwu,&nbsp;Otto Sahat Martua Silaen","doi":"10.2478/jvetres-2023-0052","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0052","url":null,"abstract":"<p><strong>Introduction: </strong><i>Escherichia coli</i> is an opportunistic bacteria that can grow easily, produce toxins, and resist antibiotics. The phenomenon of <i>E. coli</i> developing multidrug resistance is currently the subject of extensive research. The objective of this study was to molecularly identify <i>bla</i>TEM and <i>bla</i>CTX-M genes in multidrug-resistant <i>E. coli</i> found in milk samples from dairy cattle farms in Tulungagung, Indonesia.</p><p><strong>Material and methods: </strong>One hundred and ten milk samples were collected from 45 dairy cattle farms in Tulungagung, Indonesia. Indole, methyl red, Voges-Proskauer and in citrate tests and triple iron sugar agar tests were used to identify <i>E. coli</i>. Multidrug resistance was determined in isolates through antibiotic sensitivity tests using tetracycline, streptomycin, trimethoprim, chloramphenicol and aztreonam. Extended-spectrum beta lactamase enzyme production was confirmed by double-disc synergy test (DDST). Molecular identification was performed to confirm the <i>bla</i>TEM and <i>bla</i>CTX-M genes.</p><p><strong>Results: </strong>One hundred and one (91.82%) <i>E. coli</i> strains were isolated from the samples. The antibiotic sensitivity test showed four (3.96%) multidrug-resistant (MDR) and one (0.99%) ESBL-positive <i>E. coli</i> by DDST confirmation. There were three (77.78%) <i>bla</i>TEM genes and one (0.99%) <i>bla</i>CTX-M gene discovered in the MDR <i>E. coli</i> isolates using PCR for molecular identification.</p><p><strong>Conclusion: </strong>The findings of the <i>bla</i>TEM and <i>bla</i>CTX-M genes encoding ESBL <i>E. coli</i> in dairy cattle milk in Tulungagung, Indonesia is concerning and argues for prompt action to stop the emergence of antibiotic resistance which has an impact on public health.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ef/89/jvetres-67-3-jvetres-2023-0052.PMC10541667.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41134247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of SNP markers for canine mammary gland tumours in females based on a genome-wide association study - preliminary results.","authors":"Anna Mucha,&nbsp;Błażej Nowak,&nbsp;Stanisław Dzimira,&nbsp;Bartłomiej Liszka,&nbsp;Magdalena Zatoń-Dobrowolska","doi":"10.2478/jvetres-2023-0040","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0040","url":null,"abstract":"<p><strong>Introduction: </strong>The development of genetic research over recent decades has enabled the discovery of new genetic markers, such as single nucleotide polymorphisms (SNPs). This, as well as the full sequencing of the dog genome, has enabled genome-wide association studies (GWAS) to be used in the search for genetic causes of canine mammary tumours (CMTs).</p><p><strong>Material and methods: </strong>Genotypic data containing 175,000 SNPs, which had been obtained using the Illumina CanineHD BeadChip microarray technique, were available for analysis in this study. The data concerned 118 bitches, including 36 animals with CMT, representing various breeds and age groups. Statistical analysis was performed in two steps: quality control of genotyping data and genome-wide association analysis based on dominant, recessive, overdominant, codominant, and log-additive models with the single SNP effects.</p><p><strong>Results: </strong>A total of 40 different SNPs significantly associated with CMT appearance were detected. Moreover, twelve SNPs showed statistical significance in more than one model. Of all the significant SNPs, two, namely <i>BICF2G630136001</i> in the overdominant model and <i>TIGRP2P107898_rs9044787</i> in the log-additive model, reached the 5^-8 significance level. The other SNPs were significant to a 1^-5 level.</p><p><strong>Conclusion: </strong>In the group of SNPs indicated as significant in the GWAS analysis, several transpired to be localised within genes that may play an important role in CMT.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f5/79/jvetres-67-3-jvetres-2023-0040.PMC10541661.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porcine carcasses as an underestimated source of antimicrobial resistant <i>Campylobacter coli</i>.","authors":"Jacek Osek,&nbsp;Kinga Wieczorek","doi":"10.2478/jvetres-2023-0047","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0047","url":null,"abstract":"<p><strong>Introduction: </strong>Campylobacteriosis is the most common human foodborne bacterial infection worldwide and is caused by bacteria of the <i>Camplylobacter</i> genus. The main source of these bacteria is poultry, but other food-producing animals such as pigs are also responsible for human infections. An increasing number of strains with resistance to fluoroquinolones and other antimicrobials such as macrolides were recently noted. The aim of the study was to investigate <i>Campylobacter</i> contamination of porcine carcasses and determine the antimicrobial resistance of the obtained isolates.</p><p><strong>Material and methods: </strong>A total of 534 swabs from carcasses of pigs slaughtered in Poland during 2019-2022 were tested for <i>Campylobacter</i> spp.</p><p><strong>Results: </strong><i>Campylobacter</i> was detected in 164 (30.7%) carcasses; among them 149 (90.8%) were classified as <i>C. coli</i> and the remaining 15 (9.2%) samples were <i>C. jejuni</i>-positive. Because a low number of <i>C. jejuni</i> isolates were identified, only the <i>C. coli</i> isolates were subjected to antimicrobial resistance analysis. The majority of these isolates were resistant to streptomycin (94.0%), ciprofloxacin (65.8%) and tetracycline (65.1%). A total of 94 (63.1%) strains displayed antimicrobial multiresistance patterns and were mainly resistant to fluoroquinolones, aminoglycosides and tetracyclines (74; 49.7% of the isolates tested).</p><p><strong>Conclusion: </strong>The obtained results showed that pig carcasses may be contaminated with antimicrobial-resistant <i>C. coli</i>.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bf/80/jvetres-67-3-jvetres-2023-0047.PMC10541655.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41142226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibody response to 1.0 and 0.5 mL doses of an inactivated bacterial vaccine against bovine respiratory disease in young Holstein calves: a field trial.","authors":"Kazusa Mori,&nbsp;Toshihide Kato,&nbsp;Keigo Kosenda,&nbsp;Osamu Yokota,&nbsp;Hiromichi Ohtsuka","doi":"10.2478/jvetres-2023-0037","DOIUrl":"https://doi.org/10.2478/jvetres-2023-0037","url":null,"abstract":"<p><strong>Introduction: </strong>Early vaccination of cattle with an inactivated commercial bacterial vaccine against bovine respiratory disease has been reported to increase antibody production and can alleviate the disease. However, its dosage has been little investigated in young Holstein calves. This study addresses the need to establish guide values for vaccine dosage in these animals.</p><p><strong>Material and methods: </strong>Healthy calves received an inactivated vaccine for <i>Histophilus somni</i>, <i>Pasteurella multocida</i> and <i>Mannheimia haemolytica</i> intramuscularly at the ages of 1 and 4 weeks. Administered vaccine doses were 1.0 mL for the primary and booster vaccinations (1.0 + 1.0 group), 0.5 mL for the primary and 1.0 mL for the booster vaccination (0.5 + 1.0 group), or 0.5 mL for both vaccinations (0.5 + 0.5 group).</p><p><strong>Results: </strong>Differences in the vaccine responses between the 1.0 + 1.0 group and 0.5 + 1.0 group were minor. However, the number of calves with a positive vaccine response to <i>H. somni</i> in the 0.5 + 0.5 group was less than half of that in the 1.0 + 1.0 and 0.5 + 1.0 groups. In logistic regression analysis, although the booster vaccination dose was positively correlated with seropositivity for <i>H. somni</i>, the primary vaccination dose was not correlated with vaccine response. The number of calves with positive vaccine responses to <i>M. haemolytica</i> was low even after booster vaccination regardless of the dose.</p><p><strong>Conclusion: </strong>The dose of 0.5 mL can be used for primary vaccinations in newborn Holstein calves, but 1.0 mL may be required for booster vaccinations.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d6/05/jvetres-67-3-jvetres-2023-0037.PMC10541654.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41131762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (<i>Apis mellifera</i> L.) colonies?","authors":"Eliška Čukanová, Jana Prodělalová, Miroslava Palíková, Kristýna Kováčová, Petr Linhart, Ivana Papežíková","doi":"10.2478/jvetres-2023-0046","DOIUrl":"10.2478/jvetres-2023-0046","url":null,"abstract":"<p><strong>Introduction: </strong>Honey bee viruses have been shown to negatively affect the vigour and longevity of European honey bees (<i>Apis mellifera</i> L). In the present work, beehive materials were tested for their potential to serve as non-invasive samples for honey bee virus detection.</p><p><strong>Material and methods: </strong>Honey, pollen, hive debris, hive grid smears and forager honey bees were collected from 24 hives at four locations in the Czech Republic. Deformed wing virus (DWV), acute bee paralysis virus (ABPV), sacbrood virus (SBV) and black queen cell virus (BQCV) were detected using a reverse transcription PCR (RT-PCR) and real-time quantitative RT-PCR and the results for bees and alternative materials compared.</p><p><strong>Results: </strong>All forager bee samples contained DWV, BQCV and SBV and 54.2% had ABPV. When comparing beehive materials to bees, the most promising results were obtained from honey and pollen samples, with BQCV and SBV detected in all honey samples and ABPV in 12.5%. Detection of SBV was achieved in 91.6% of pollen samples, detection of BQCV in 87.5% and detection of DWW in 75%. The results for debris and smears were less consistent with the viral profile of the forager samples.</p><p><strong>Conclusion: </strong>The best candidate materials for honey bee virus detection in a non-invasive technique are honey and pollen.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/83/a2/jvetres-67-3-jvetres-2023-0046.PMC10541673.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41121245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}