Urszula Lisiecka, Piotr Brodzki, Anna Śmiech, Katarzyna Michalak, Stanisław Winiarczyk, Beata Żylińska, Łukasz Adaszek
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The phenotypic features of peripheral blood leukocytes were evaluated by flow cytometry.</p><p><strong>Results: </strong>There was a significant difference in the mean percentages of CD3<sup>+</sup> lymphocytes between healthy (66.7%) and metastatic dogs (46.1%), and between tumour-bearing non-metastatic (66.6%) and metastatic dogs. There was also a significant difference in CD4<sup>+</sup> T helper cell percentages between healthy dogs (40.4%) and dogs with metastases (23.2%), and between the latter and dogs without them (35.5%). In the case of CD21<sup>+</sup> lymphocyte subsets, a significant difference was noted between healthy animals (10.9%) and those with metastases (20.1%), and between the latter and patients without metastases (8.5%). There were also significant differences in CD3<sup>+</sup>/CD21<sup>+</sup> ratios between the group with metastases (3.0), the healthy group (7.8), and the group without metastases (8.5). Similarly, a significant difference was noted in CD4<sup>+</sup>/CD8<sup>+</sup> ratios between animals with metastases (1.4), bitches in the control group (2.2), and dogs without metastases (1.9).</p><p><strong>Conclusion: </strong>Peripheral blood leukocyte phenotypic characteristics are putative novel biomarkers. These findings may be useful in future studies improving mammary tumour diagnostic procedures, especially in metastasis detection.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210351/pdf/","citationCount":"0","resultStr":"{\"title\":\"The diagnostic value of selected immune parameters in peripheral blood of dogs with malignant mammary tumours - a preliminary study.\",\"authors\":\"Urszula Lisiecka, Piotr Brodzki, Anna Śmiech, Katarzyna Michalak, Stanisław Winiarczyk, Beata Żylińska, Łukasz Adaszek\",\"doi\":\"10.2478/jvetres-2024-0035\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The main adaptive immune cells are T and B lymphocytes and they play key roles in the induction of immune responses against canine mammary tumours. Investigating these cell subpopulations may lead to more precise diagnosis of these malignancies.</p><p><strong>Material and methods: </strong>The percentages of CD3<sup>+</sup>, CD4<sup>+</sup> and CD8<sup>+</sup> T cells and of CD21<sup>+</sup> B cells in the peripheral blood of bitches with malignant mammary tumours were compared with those in the blood of healthy animals. The phenotypic features of peripheral blood leukocytes were evaluated by flow cytometry.</p><p><strong>Results: </strong>There was a significant difference in the mean percentages of CD3<sup>+</sup> lymphocytes between healthy (66.7%) and metastatic dogs (46.1%), and between tumour-bearing non-metastatic (66.6%) and metastatic dogs. There was also a significant difference in CD4<sup>+</sup> T helper cell percentages between healthy dogs (40.4%) and dogs with metastases (23.2%), and between the latter and dogs without them (35.5%). 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引用次数: 0
摘要
导言:T淋巴细胞和B淋巴细胞是主要的适应性免疫细胞,它们在诱导针对犬乳腺肿瘤的免疫反应中发挥着关键作用。对这些细胞亚群进行研究可能有助于更精确地诊断这些恶性肿瘤:将患有恶性乳腺肿瘤的母犬外周血中 CD3+、CD4+ 和 CD8+ T 细胞以及 CD21+ B 细胞的百分比与健康动物血液中的百分比进行比较。流式细胞仪对外周血白细胞的表型特征进行了评估:结果:健康犬(66.7%)和转移犬(46.1%)之间以及肿瘤未转移犬(66.6%)和转移犬之间的 CD3+ 淋巴细胞平均百分比存在明显差异。健康犬(40.4%)和有转移瘤犬(23.2%)之间的 CD4+ T 辅助细胞百分比也有显著差异,后者与无转移瘤犬(35.5%)之间也有显著差异。在 CD21+ 淋巴细胞亚群方面,健康动物(10.9%)与转移动物(20.1%)之间存在显著差异,后者与未转移患者(8.5%)之间也存在显著差异。转移组(3.0)、健康组(7.8)和无转移组(8.5)之间的 CD3+/CD21+ 比率也存在明显差异。同样,有转移瘤的动物(1.4)、对照组母犬(2.2)和无转移瘤的犬只(1.9)之间的 CD4+/CD8+ 比率也存在明显差异:结论:外周血白细胞表型特征是一种潜在的新型生物标记物。结论:外周血白细胞表型特征是一种潜在的新型生物标志物,这些发现可能有助于今后改进乳腺肿瘤诊断程序的研究,尤其是在转移瘤检测方面。
The diagnostic value of selected immune parameters in peripheral blood of dogs with malignant mammary tumours - a preliminary study.
Introduction: The main adaptive immune cells are T and B lymphocytes and they play key roles in the induction of immune responses against canine mammary tumours. Investigating these cell subpopulations may lead to more precise diagnosis of these malignancies.
Material and methods: The percentages of CD3+, CD4+ and CD8+ T cells and of CD21+ B cells in the peripheral blood of bitches with malignant mammary tumours were compared with those in the blood of healthy animals. The phenotypic features of peripheral blood leukocytes were evaluated by flow cytometry.
Results: There was a significant difference in the mean percentages of CD3+ lymphocytes between healthy (66.7%) and metastatic dogs (46.1%), and between tumour-bearing non-metastatic (66.6%) and metastatic dogs. There was also a significant difference in CD4+ T helper cell percentages between healthy dogs (40.4%) and dogs with metastases (23.2%), and between the latter and dogs without them (35.5%). In the case of CD21+ lymphocyte subsets, a significant difference was noted between healthy animals (10.9%) and those with metastases (20.1%), and between the latter and patients without metastases (8.5%). There were also significant differences in CD3+/CD21+ ratios between the group with metastases (3.0), the healthy group (7.8), and the group without metastases (8.5). Similarly, a significant difference was noted in CD4+/CD8+ ratios between animals with metastases (1.4), bitches in the control group (2.2), and dogs without metastases (1.9).
Conclusion: Peripheral blood leukocyte phenotypic characteristics are putative novel biomarkers. These findings may be useful in future studies improving mammary tumour diagnostic procedures, especially in metastasis detection.
期刊介绍:
Journal of Veterinary Research (formerly Bulletin of the Veterinary Institute in Pulawy) is a quarterly that publishes original papers, review articles and short communications on bacteriology, virology, parasitology, immunology, molecular biology, pathology, toxicology, pharmacology, and biochemistry. The main emphasis is, however, on infectious diseases of animals, food safety and public health, and clinical sciences.