Journal of Veterinary Research最新文献

{"title":"Occurrence of virulence factors and antimicrobial susceptibility of <i>Citrobacter freundii</i> isolated from diseased ornamental fish in Poland.","authors":"Anna Pastuszka, Leszek Guz, Krzysztof Puk, Dorota Pietras-Ożga","doi":"10.2478/jvetres-2025-0017","DOIUrl":"10.2478/jvetres-2025-0017","url":null,"abstract":"<p><strong>Introduction: </strong><i>Citrobacter freundii</i> strains, like other representatives of the same genus, are often described as aetiological factors of diseases in humans and animals, including farmed and ornamental fish. The aim of the study was the isolation and identification of <i>C. freundii</i> from ornamental fish in Poland and investigation of the virulence factors and antibiotic resistance among the isolates.</p><p><strong>Material and methods: </strong>Twenty <i>C. freundii</i> isolates were isolated from swab samples obtained from diseased ornamental fish of 16 different species in Poland. The bacteria were initially identified to the species level by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and the results were confirmed based on sequencing of a fragment of the <i>16S rRNA</i> gene and biochemical profile analysis using the API-20E system. The antibiotic susceptibility profile was evaluated by the disc-diffusion method.</p><p><strong>Results: </strong>Most isolates were resistant to tetracycline (65%), ciprofloxacin (65%), nalidixic acid (60%) and doxycycline (50%). All of them were resistant to ampicillin. As many as 13/20 isolates (65%) carried the <i>viaB</i> gene encoding the Vi antigen and 9/20 (45%) carried the class 1 integron-encoding gene. The <i>hlyA</i> gene encoding the ability to produce α-haemolysins was not detected in any of the isolates tested.</p><p><strong>Conclusion: </strong>This is the first study to describe in detail the identification and occurrence of virulence factors and antibiotic resistance among <i>C. freundii</i> isolates of ornamental fish in Poland. The results indicate the need for further monitoring of the bacterium's presence.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"17-26"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of probiotics on immune cells in young Japanese Black calves responding to vaccination against bacterial respiratory diseases.","authors":"Shogo Takeda, Hiromichi Ohtsuka, Keigo Kosenda","doi":"10.2478/jvetres-2025-0013","DOIUrl":"10.2478/jvetres-2025-0013","url":null,"abstract":"<p><strong>Introduction: </strong>The vaccination against bacterial respiratory diseases in calves has been generally recognised as useful for the prevention of infections. Inducing an immunological response after vaccination is important for obtaining protection from infections. The aim of the study was to investigate the effects of probiotics on the immunological response to vaccination against bacterial respiratory diseases in young Japanese Black calves.</p><p><strong>Material and methods: </strong>Twenty-four Japanese Black calves were randomly divided into two groups (12 calves for the research group and 12 calves for the control group) on the seventh day of life (dol). The research group received 30 g per day of live bacteria mix consisting of <i>Streptococcus faecalis, Clostridium butyricum</i> and <i>Bacillus mesentericus</i> until the 63^rd dol. The control group did not receive the bacteria mix. All calves were vaccinated against bacterial respiratory diseases twice, at 21 and 42 dol. Blood samples were obtained from all calves at 7, 21, 42 (prior to the second vaccination), 45, 49 and 63 dol for determination of antibody titres, leukocyte numbers and cytokine genes.</p><p><strong>Results: </strong>Lymphocyte counts, T cell (CD3⁺, CD4⁺ and CD8⁺ cell) counts and relative expressions of cytokine genes (interleukin (IL)-4 and IL-17A) at 45, 49 and 63 dol were significantly higher in the research group compared than in the control group.</p><p><strong>Conclusion: </strong>The addition of probiotics to young Japanese Black calves' feed promoted an immunological reaction to vaccination against bacterial respiratory diseases.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"27-33"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The tissue distribution of nucleobindin-2/nesfatin-1 in the reproductive organs of bitches with regard to the animal's age and body weight.","authors":"Marta Rybska, Marek Skrzypski, Karolina Pusiak, Tatiana Wojciechowicz, Adam Mieldzioc","doi":"10.2478/jvetres-2025-0015","DOIUrl":"10.2478/jvetres-2025-0015","url":null,"abstract":"<p><strong>Introduction: </strong>Neuropeptide nesfatin-1, a nucleobindin-2 derivative, plays a role in regulating food intake, energy metabolism and body weight. It also interacts with the hypothalamic-pituitary-gonadal axis and has functions in the reproductive system. However, its impact on the canine reproductive tract has not been well documented. This study aimed to investigate the potential role of nesfatin-1 in canine ovarian activity and uterine function.</p><p><strong>Material and methods: </strong>Tissue and peripheral blood samples were collected from 60 bitches of various ages and body condition scores (BCS). Analyses included real-time PCR, immunofluorescence examinations and ELISA tests.</p><p><strong>Results: </strong>Higher level of nucleobindin-2 mRNA were found in the ovarian tissue of both younger and elderly overweight dogs (BCS > 5/9). The elevated expression of nesfatin-1 was observed in the uterine tissues of overweight dogs (BCS > 5/9) compared to its expression in animals in optimal body condition (BCS = 4/9). This finding was consistent with higher nesfatin-1 levels in the peripheral blood of overweight dogs.</p><p><strong>Conclusion: </strong>The distribution and expression of nesfatin-1 in canine reproductive organs vary depending on the animal's age and body weight. The role of nesfatin-1 in the reproductive system is influenced by the animal's body condition and the extent of surplus adipose tissue, which may have significant implications for reproductive functions.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"141-150"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of drug-resistant staphylococci isolated from milk of lambed ewes during the perinatal period.","authors":"Agata Hahaj-Siembida, Aneta Nowakiewicz, Monika Greguła-Kania, Mariola Bochniarz, Aleksandra Trościańczyk, Marcelina Osińska","doi":"10.2478/jvetres-2025-0014","DOIUrl":"10.2478/jvetres-2025-0014","url":null,"abstract":"<p><strong>Introduction: </strong>Staphylococci are still a challenge in veterinary medicine, as they are one of the aetiological factors causing clinical and subclinical mastitis in small ruminants. The aim of the study was to analyse the occurrence of staphylococci in milk obtained from Świniarka (SW) and Uhruska (UHR) sheep and to characterise their drug resistance and virulence.</p><p><strong>Material and methods: </strong>In total, 50 milk samples were collected from ewes seven days after parturition. Drug susceptibility analysis was performed based on Clinical and Laboratory Standards Institute standards and demonstration of the presence of resistance genes was attempted.</p><p><strong>Results: </strong><i>Staphylococcus</i> spp. strains were identified in 70% of the samples, and 57.2% of the strains were <i>S. aureus</i>. Most of the tested strains (88.6%) were resistant to at least one antimicrobial, and resistance to tetracycline and erythromycin was the dominant type in <i>S. aureus</i> strains. Coagulase-negative species (CoNS) exhibited resistance predominantly to penicillin, cefoxitin and tetracycline (86.6%, 73.3% and 46.6%, respectively).The presence of the <i>mec</i>C gene was detected in four cefoxitin resistant strains. In tetracycline- and chloramphenicol-resistant strains, phenotypic and genotypic resistance was statistically significantly more common among strains isolated from UHR than SW.</p><p><strong>Conclusion: </strong>The present study highlights the problem of potential subclinical mastitis caused by drug-resistant strains of <i>S. aureus</i> and other CoNS species in lambed ewes. However, the occurrence of virulence factors in these strains is very rare.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"41-50"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology and genetic analysis of <i>Oestrus ovis</i> from slaughtered sheep in Sulaymaniyah province, Iraq.","authors":"Aram Ahmad Mohammed, Taib Ahmed Hama Soor","doi":"10.2478/jvetres-2025-0019","DOIUrl":"10.2478/jvetres-2025-0019","url":null,"abstract":"<p><strong>Introduction: </strong>Oestrosis is a type of nasal myiasis that is caused by larvae of flies from the <i>Oestrus</i> genus and is a disease of economic significance in small ruminants. The research aimed to investigate the prevalence of oestrosis and detect differences in the <i>COX1</i> gene among haplotypes found in Sulaymaniyah, in the Kurdistan region of Iraq.</p><p><strong>Material and methods: </strong>The research was conducted in a Sulaymaniyah abattoir from September 2023 to August 2024. The heads of 328 sheep were carefully incised and inspected to record the larvae of <i>Oestrus ovis</i>. A conventional polymerase chain reaction and sequencing of the <i>COX1</i> gene were used for diagnosis and genetic analysis of <i>O. ovis</i>.</p><p><strong>Results: </strong>The rate of oestrosis was 22.25% and the disease was significantly (P-value < 0.05) higher in imported breeds (26.50%) than the local breed (15.65%), in adults (26.88%) than in young animals (16.19%), in females (27.05%) than in males (17.08%), and in the summer (34.09%) than in other seasons of the year. Comparison of the sequences of the conservative <i>COX1</i> gene of the parasite led to identification of five different haplotypes in the research area. Two of the haplotypes were previously recorded internationally, while three new haplotypes associated with five novel mutations were recorded for the first time in the study region.</p><p><strong>Conclusion: </strong>A phylogenetic analysis revealed a strong relationship among <i>O. ovis</i> populations from various countries. The current research offered valuable molecular data for <i>O. ovis</i> species, essential for evaluating phylogenetic relationships and identifying these parasites at a molecular level.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"51-58"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of lipopolysaccharide administration on thymus damage, antioxidant capacity and immune function in weaned piglets.","authors":"Lingna Bai, Yijie Jiang, Xi Li, Wanting Yu, Wenlu Zhu, Guotong Zhao, Tingyu Yang, Yunxiao Zhou, Jinyan Li, Yong Li","doi":"10.2478/jvetres-2025-0018","DOIUrl":"10.2478/jvetres-2025-0018","url":null,"abstract":"<p><strong>Introduction: </strong>Piglets are vulnerable to stress during weaning because of changes in the feeding environment, nutrients, and other growth-impacting conditions. In this study, stress injury was modelled by continuous intraperitoneal injection of lipopolysaccharide (LPS) and was used to investigate the dynamics of antioxidant indices and immunoinflammatory factors in the piglet thymus.</p><p><strong>Material and methods: </strong>Forty-eight weaned piglets were divided into an LPS group and a control group. One group was injected with LPS solution (100 μg/kg) and the other with sterile saline daily. The experiment ran over 13 days, and six piglets from each group were euthanised for necropsy on days 1, 5, 9 and 13. Thymic tissues were collected, and the antioxidant indices and mRNA expression levels of related genes were measured by enzyme activity assay and reverse-transcription quantitative PCR.</p><p><strong>Results: </strong>In the LPS group, catalase activities were significantly increased on days 1 and 5, that of superoxide dismutase was significantly higher on day 9 and glutathione activity was elevated throughout. Messenger RNA (mRNA) expression of the toll-like receptor 4 (TLR4) pathway, interleukin (IL) 6, and IL-2 increased in the thymus on day 1. By day 5, the mRNA expression of the TLR pathway, the janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway, the kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) pathway, tumour necrosis factor α, IL-10, IL-6 and IL-2 were decreased. On day 13, the mRNA expression levels of the TLR4 and Keap1/Nrf2 pathways, TNF-α, IL-10 and IL-6 increased again.</p><p><strong>Conclusion: </strong>Continuous LPS induction led to high activation of the thymic immune system in piglets during the prophase. However, this activation was accompanied by atrophy and immunosuppression mid-experiment. Nevertheless, the immune function gradually recovered in the later stages.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"111-119"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test.","authors":"Marcin Mickiewicz, Zofia Nowek, Michał Czopowicz, Agata Moroz-Fik, Adrian-Valentin Potărniche, Kinga Biernacka, Olga Szaluś-Jordanow, Paweł Górski, Alistair Antonopoulos, Iwona Markowska-Daniel, Marián Várady, Jarosław Kaba","doi":"10.2478/jvetres-2025-0016","DOIUrl":"10.2478/jvetres-2025-0016","url":null,"abstract":"<p><strong>Introduction: </strong>Dimethyl sulfoxide (DMSO) is an amphipathic solvent for molecules in <i>in vitro</i> tests for detection of anthelmintic resistance of gastrointestinal nematodes (GIN). It has been shown to have a concentration-dependent detrimental effect on <i>Caenorhabditis elegans</i>, a free-living nematode. If GIN are likewise affected, using DMSO in egg-hatch test and larval development test (LDT) may confound their results. Therefore, the DMSO concentration was determined at which it exerted an inhibitory effect on GIN larval development to the third stage.</p><p><strong>Material and methods: </strong>A standard LDT was performed in 30 replications at DMSO concentrations of 0.0% (control), 0.6%, 1.3%, 2.6%, 5.2%, 10.4%, and 20.8%. The numbers of all developmental stages of <i>Haemonchus contortus, Trichostrongylus</i> spp. and <i>Oesophagostomum</i> spp. (unhatched eggs, larvae of the first, second and third stages (L1-L3) were determined, the proportion of L3 (the percentage of larval development - PD) was calculated and L3 were identified at the species or genus level. A five-parameter logistic curve was fitted to the observed PDs and modelled the DMSO-larval development relationship.</p><p><strong>Results: </strong>The PD significantly decreased with increasing DMSO concentration and was significantly reduced at the 2.6% concentration. The median inhibitory concentration (IC50) was 3.79%, the concentration for 10% inhibition (IC10) was 1.75% and for 90% inhibition (IC90) was 8.20%. The percentage of L1 and L2 followed an analogical but opposite pattern to that of PD and was complementary to it at each DMSO concentration. The unhatched egg percentage was rarely >1% and showed no pattern.</p><p><strong>Conclusion: </strong>At ≥2.6% concentration, DMSO significantly inhibited the L3 development of all three GIN species. It had a practically important inhibitory effect (IC10) at as low concentration as 1.75%. At lower concentrations, DMSO did not appear to inhibit larval development. The compound did not seem to exert an <i>in vitro</i> ovicidal effect regardless of the concentration.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"83-90"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parasitic infections in the organic beef cattle herds of southern Poland during the grazing season, with the first record of <i>Calicophoron daubneyi</i> (Dinnik, 1962) in the country.","authors":"Paweł Nosal, Jerzy Kowal, Marta Basiaga, Andrzej Węglarz","doi":"10.2478/jvetres-2025-0012","DOIUrl":"10.2478/jvetres-2025-0012","url":null,"abstract":"<p><strong>Introduction: </strong>Grazing cattle are vulnerable to the harmful effects of gastrointestinal parasites. Organically farmed cattle are even more so because conventional antiparasitic treatments are restricted, yet parasite infection patterns in Polish organic herds remain poorly documented.</p><p><strong>Material and methods: </strong>Imported beef cattle were studied during the pasture season in four organic herds in southern Poland. The McMaster quantitative flotation method was used to estimate infection prevalence (P, %) coproscopically and to quantify intensities of coccidia oocyst output (Ic, OPG) and nematode egg output (In, EPG) per gram of faeces. The qualitative sedimentation method was applied to assess the presence of digenean eggs. Coccidial species of the <i>Eimeria</i> genus were identified by sporulation, and nematodes of the Strongylida order by larvoscopy. Digenean Paramphistomatidae were identified by morphological examination of adult fluke specimens obtained at slaughter from a sick heifer in one of the studied herds and by molecular analysis of the flukes' internal transcribed spacer 2 ribosomal DNA.</p><p><strong>Results: </strong>The prevalence of <i>Eimeria</i> infection was P = 28.9 (23.8-34.5)%, and the mean Ic was 287 (113-793) OPG. Calves were most heavily infected, mainly with <i>E. bovis</i> and <i>E. zuernii</i>. The prevalence of nematode infections reached P = 46.0 (40.2-51.5)%, and the mean In was 113 (88-147) EPG. <i>Haemonchus placei</i> dominated over <i>Ostertagia</i> sp. and <i>Trichostrongylus axei</i>, and the most infected were first-time grazing yearlings. Paramphistome eggs were confirmed in only one herd. Morphological and PCR analysis of the adult rumen flukes revealed the presence of <i>Calicophoron daubneyi</i> (Dinnik, 1962) in this herd.</p><p><strong>Conclusion: </strong>This is the first Polish evidence of <i>C. daubneyi</i>, and it heralds an enhanced surveillance need regarding this highly pathogenic digenean.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"59-69"},"PeriodicalIF":1.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pathological characteristics of enzootic nasal adenocarcinoma in goats.","authors":"Lingxu Li, Zhen Wang, Weiling Qi, Yingjun Lv, Dawei Yao","doi":"10.2478/jvetres-2025-0010","DOIUrl":"10.2478/jvetres-2025-0010","url":null,"abstract":"<p><strong>Introduction: </strong>Enzootic nasal adenocarcinoma (ENA) is a nasal cancer that occurs in goats and sheep infected by enzootic nasal tumour virus. Pathologic examinations are useful for distinguishing tumours from inflammatory hyperplasia. The aim of this study was to describe the pathological characteristics of ENA.</p><p><strong>Material and methods: </strong>Caprine tumour samples were collected for pathological examination. The tissue sections were stained with haematoxylin-eosin and periodic acid-Schiff (PAS) and processed for immunohistochemical staining. Tumour samples were also processed for routine transmission electron microscopy (TEM).</p><p><strong>Results: </strong>The histopathological structure of the tumours exhibited both papillary formations in the superficial regions and tubular or acinar formations in the deeper layers, representing distinct structural patterns within the same adenocarcinoma. The tumour cells were positive for PAS, and mitotic figures were rare. Low-differentiated cancer nests and epithelial-mesenchymal transition phenomena were observed. Immunohistochemical analysis showed that the tumour cells were strongly positive for pancytokeratin and cytokeratin (CK)18, moderately positive for CK7, and did not express olfactory marker protein. The Kiel 67 labelling index was approximately 23%. Retrovirus-like particles were distributed inside and outside of acinar tumour cells in TEM.</p><p><strong>Conclusion: </strong>The origin site of ENA is the epithelium of the nasal glandular tubules. This cancer is a low-grade adenocarcinoma with malignant potential. Cytokeratin 7 and CK18 can be considered immunophenotypes for identifying ENA tumour cells.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"151-157"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of primary cell cultures from canine mammary gland malignant tumours: a preliminary study.","authors":"Patŕicia Petrouškova, Nikola Hudáková, Viera Almášiová, Alexandra Valenčáková, L'ubica Horňáková, Mykhailo Huniadi, Daša Čížková","doi":"10.2478/jvetres-2025-0007","DOIUrl":"10.2478/jvetres-2025-0007","url":null,"abstract":"<p><strong>Introduction: </strong>Canine mammary gland cancer (CMGC) is the most common neoplastic condition in bitches and is often fatal. There are limited treatment options for CMGC. Primary cell cultures from mammary tumours are promising preclinical <i>in vitro</i> models in which to study personalised treatment approaches. This preliminary study aimed to establish primary cell cultures from two canine mammary gland neoplasms: a common solid adenocarcinoma and a rare carcinosarcoma.</p><p><strong>Material and methods: </strong>Tumour masses were collected from a 13-year-old and a 16-year-old German shepherd. Tumour cells were isolated by mechanical disaggregation and enzymatic digestion of masses with 0.05% type IV collagenase. Primary cell cultures were validated by immunocytochemistry for specific markers including mucin 1 (MUC1), cytokeratin 8 and 18 (CK8/18) and Kiel 67 (Ki-67).</p><p><strong>Results: </strong>Primary cell cultures achieved confluency by day 7 of culture, displaying polygonal cellular morphology. Cultures of both cell types exhibited strong positivity for MUC1 of >99% and high Ki-67 proliferation activity of 43.1% ± 0.5% in the solid adenocarcinoma-derived positive cells and 87.9% ± 2.7% in the carcinosarcoma-derived positive cells. Positivity was observed for CK8/18 of 98.1% ± 0.3% in cells derived from solid adenocarcinoma and 31.6% ± 1.5% in cells derived from carcinosarcoma.</p><p><strong>Conclusion: </strong>With further characterisation, the primary cell cultures established in this study can be expected to show considerable potential as foundational <i>in vitro</i> models for cancer research.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"159-168"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}