Journal of Veterinary Research最新文献

{"title":"The herd-level prevalence of Fasciola hepatica infection in the goat population of Poland","authors":"M. Mickiewicz, Z. Nowek, M. Czopowicz, A. Moroz-Fik, K. Biernacka, A. Potărniche, O. Szaluś-Jordanow, Paweł Górski, T. Nalbert, Krzysztof Buczek, Aija Mālniece, Iwona Markowska-Daniel, J. Kaba","doi":"10.2478/jvetres-2024-0044","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0044","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000 Fasciola hepatica, also known as the common liver fluke, is a globally distributed trematode parasite responsible for high economic losses in ruminants. Infection with F. hepatica occurs in Polish cattle and sheep; however, very little is known about its occurrence in goats. Therefore, a serological and coproscopic survey was carried out in Polish goats to determine the herd-level prevalence of F. hepatica infection in the goat population of Poland.\u0000 \u0000 \u0000 \u0000 Between 2014 and 2022, 33 randomly selected goat herds were serologically screened in the regions of Poland for which risk of F. hepatica infection was estimated as increased based on the spatial distribution model developed within the frame of the GLOWORM project. Virtually all adult goats (>1 year-old) were tested using a commercial MM3-SERO ELISA. Risk factors for seropositive herd status were analysed in contingency tables. Also, faecal samples from 214 goat herds monitored for gastrointestinal nematode infections and anthelmintic resistance were examined using a sedimentation method.\u0000 \u0000 \u0000 \u0000 At least one seropositive goat was detected in 11 of 33 herds, indicating herd-level seroprevalence of 33.3% (95% confidence interval (CI 95%): 19.7%–50.4%). At the animal level, only 17 of 1,464 tested goats were seropositive (1.2%, CI 95%: 0.7%–1.9%). The within-herd seroprevalence ranged from 0.8% to 11.1%. The serological status of the herd was not significantly associated with the characteristics of the herd or the extent of contact with sheep. In one herd, located in central Poland, a single positive faecal sample was found indicating a herd-level prevalence of F. hepatica infection of 0.5% (CI 95%: 0.1%–2.6%). The animal’s post-mortem examination revealed liver lesions typical of chronic fasciolosis.\u0000 \u0000 \u0000 \u0000 \u0000 F. hepatica infection occurs sporadically in Polish goat population and its prevalence is much lower than in cattle or sheep. Therefore, treatment or prevention of fasciolosis should only be considered if it has been reliably confirmed by an accurate diagnostic test. This applies also to goats inhabiting geographical areas where F. hepatica infection appears to be widespread in cattle and sheep, very likely due to the fact that goats avoid wet areas.\u0000","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141928590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Murine hepatic proteome adaptation to high-fat diets with different contents of saturated fatty acids and linoleic acid : α-linolenic acid polyunsaturated fatty acid ratios","authors":"K. Liput, Adam Lepczyński, E. Poławska, Magdalena Ogłuszka, Rafał Starzyński, P. Urbański, A. Nawrocka, Aneta Jończy, D. Pierzchała, C. Pareek, Marcin Gołyński, Grzegorz Woźniakowski, Urszula Czarnik, M. Pierzchała","doi":"10.2478/jvetres-2024-0041","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0041","url":null,"abstract":"\u0000 \u0000 \u0000 Some health disorders, such as obesity and type 2 diabetes, are associated with a poor diet and low quality of the fat in it. The type and duration of the diet have an impact on the liver. This investigation uses the proteomic approach to identify changes in the mouse liver protein profile in adaptation to high-fat diets with different saturated fatty acid contents and linoleic acid (18:2n-6) to α-linolenic acid (18:3n-3) fatty acid ratios.\u0000 \u0000 \u0000 \u0000 Four groups of male mice were fed different diets: one standard diet and three high-fat diets were investigated. After six months on these diets, the animals were sacrificed for liver dissection. Two-dimensional electrophoresis was used to separate the complex liver protein mixture, which enabled the separation of proteins against a wide, 3–10 range of pH and molecular weights of 15–250 kDa. Protein profiles were analysed in the PDQuest Advanced 8.0.1 program. Differentially expressed spots were identified using matrix-assisted laser desorption/ionisation–time-of-flight tandem mass spectrometry and peptide mass fingerprinting. The levels of identified proteins were validated using Western blotting. Transcript levels were evaluated using a real-time quantitative PCR.\u0000 \u0000 \u0000 \u0000 The analysis of mouse liver protein profiles enabled the identification of 32 protein spots differing between nutritional groups.\u0000 \u0000 \u0000 \u0000 A diet high in polyunsaturated fatty acids modulated the levels of liver proteins involved in critical metabolic pathways, including amino acid metabolism, carbohydrate metabolism and cellular response to oxidative stress.\u0000","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141927356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of probiotic administration on the phagocytic and oxidative burst activity of neutrophils and monocytes in the peripheral blood of dairy cows during different lactation periods","authors":"P. Brodzki, Hubert Gorzkoś, J. Marczuk, U. Lisiecka, Andrzej Junkuszew, L. Krakowski, M. Szczubiał, Nikodem Brodzki, Katarzyna Głodkowska","doi":"10.2478/jvetres-2024-0043","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0043","url":null,"abstract":"\u0000 \u0000 \u0000 The objective of this bovine peripheral blood study was a comparative assessment of the phagocytic activity of neutrophils and monocytes and of the intracellular killing capacity of neutrophils from cows given no probiotic and from cows which were administered a probiotic consisting of Saccharomyces cerevisiae, Lactobacillus acidophilus, Lactobacillus plantarum and Rhodopseudomonas palustris. These activity types were compared during different lactation periods.\u0000 \u0000 \u0000 \u0000 A cohort of 20 pregnant dairy cows was divided into two groups of 10. The experimental group consisted of cows fed a ration supplemented with probiotics, and the control group consisted of cows fed an unsupplemented ration. Blood was drawn six times: 7 days before dry off, 14 days before parturition, and 7, 21, 60 and 90 days postpartum (DPP). The phagocytic activity of neutrophils and monocytes and the oxidative burst activity of neutrophils were determined by flow cytometry in the peripheral blood of all examined cows.\u0000 \u0000 \u0000 \u0000 Phagocytosis testing revealed increased percentages of phagocytic neutrophils and monocytes in the experimental group at 21, 60 and 90 DPP (P-value < 0.01). The mean fluorescence intensity (MFI) values for neutrophils and monocytes were higher on all days of the study (P-value < 0.01). In oxidative burst testing, the percentages of detected neutrophils and their MFI were increased in the experimental group on all days (P-value < 0.01).\u0000 \u0000 \u0000 \u0000 The use of probiotics supported dairy cows’ immunity throughout the whole experiment. Probiotic supplementation may limit the occurrence of infectious diseases in these animals.\u0000","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141925713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunodetection of selected pancreatic hormones under intragastric administration of apelin-13, a novel endogenous ligand for an angiotensin-like orphan G-protein coupled receptor, in unweaned rats.","authors":"Sylwia Szymańczyk, Katarzyna Kras, Cezary Osiak-Wicha, Małgorzata Kapica, Iwona Puzio, Hanna Antushevich, Atsukazu Kuwahara, Ikuo Kato, Marcin B Arciszewski","doi":"10.2478/jvetres-2024-0042","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0042","url":null,"abstract":"<p><strong>Introduction: </strong>This study investigated the effects of intragastric administration of apelin-13 on the secretion of critical pancreatic hormones in a cohort of three-week-old Wistar rats. The research aimed to uncover apelin's modulatory roles in endocrine interactions dictating metabolic homeostasis during early life.</p><p><strong>Material and methods: </strong>Rats were randomly assigned to control or experimental groups, receiving apelin-13 or saline for 14 days. The study population consisted of three-week-old Wistar rats of both sexes, weighing between 20 and 25 grams. Histological examination, analysis of variance and <i>t</i>-tests were employed to assess significant differences.</p><p><strong>Results: </strong>Distinctive alterations in large islet morphology were observed, indicating a notable reduction in size. Additionally, an increase in alpha- and beta-cell density within specific islet sizes was noted, suggesting significant changes in cell populations. The study found a substantial increase in mitotic activity and a decrease in apoptosis in small and medium-sized islets post apelin-13 administration, indicating its potential role in regulating cell survival and proliferation.</p><p><strong>Conclusion: </strong>The notable reduction in large islet size coupled with increased alpha and beta cell density implies a targeted impact of apelin-13 on pancreatic cell dynamics. Also, the observed increase in mitotic activity and decrease in apoptosis in small and medium-sized islets suggest its potential regulatory role in cell survival and proliferation within the pancreatic microenvironment.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytotherapeutic potential of <i>Artemisia ludoviciana</i> and <i>Cordia boissieri</i> extracts against the dermatophyte <i>Microsporum canis</i>.","authors":"José Pablo Villarreal Villarreal, Beatriz A Medina Soto, Michel Stéphane Heya, Sergio A Galindo-Rodríguez, Uziel Castillo Velázquez, Kevin A Cárdenas Noriega, Romario García-Ponce","doi":"10.2478/jvetres-2024-0046","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0046","url":null,"abstract":"<p><strong>Introduction: </strong><i>Microsporum canis</i> is a dermatophyte that mainly affects dogs and cats. However, it can be transmitted to humans by direct contact. This makes it one of the most frequent causative agents of dermatophytosis in humans, reflecting the frequent human close relationships with pets. Conventional treatment relies on antifungal pharmacological agents. However, errors in application have led to the occurrence of fungal resistance and toxic effects. Consequently, new therapeutic alternatives are needed for <i>M</i>. <i>canis</i> infections. Plant extracts have been explored as phytotherapeutics for the treatment of dermatophyte infections, which prompted an attempt to apply extracts of the ethnopharmacologically important plants <i>Artemisia ludoviciana</i> and <i>Cordia boissieri</i>.</p><p><strong>Material and methods: </strong>Methanolic extracts of these two plants were obtained using a Soxhlet method and were characterised by phytochemical screening. Extracts were evaluated against a <i>M</i>. <i>canis</i> commercial strain (ATCC-11621) using the microdilution method described in the Clinical and Laboratory Standards Institute protocol M38-A, determining its minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). Subsequently, these concentrations were tested in a human keratinocyte human cell line.</p><p><strong>Results: </strong><i>Artemisia ludoviciana</i> and <i>C. boissieri</i> extracts showed MIC values of 2,500 and 1,250 µg/mL, and MFC values of 5,000 and 2,500 µg/mL against <i>M. canis</i>, respectively. These extracts did not inhibit HaCaT cell proliferation <i>in vitro</i>.</p><p><strong>Conclusion: </strong>The evaluated extracts showed potential for the treatment of <i>M. canis</i> fungal infections. However, further studies on their phytochemical characterisation, purification, clinical safety and formulation are required.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11424138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How does adulteration of wax foundation affect phenoloxidase and lysozyme activities as selected parameters of immunity in <i>Apis mellifera</i>?","authors":"Aneta Strachecka, Magdalena Chęć, Krzysztof Olszewski, Patrycja Staniszewska, Piotr Dziechciarz, Mariusz Gagoś","doi":"10.2478/jvetres-2024-0040","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0040","url":null,"abstract":"<p><strong>Introduction: </strong>The adulteration of wax foundation is, for many reasons, a growing problem of modern beekeeping not only in Europe but also around the world. Wax foundation contaminated with stearin addition leads to a brood die-off, while paraffin addition negatively affects the strength of combs. It is tenable that such adulterated wax foundation reduces bees' immunity. The aim of the study was to determine the activities of two bee immune enzymes, lysozyme and phenoloxidase, in the haemolymph of worker bees which had emerged from combs with wax foundations contaminated with stearin or paraffin.</p><p><strong>Material and methods: </strong>Combs built with stearin- or paraffin-adulterated wax (both adulterants at concentrations of 10%, 30% or 50%) or pure wax (0% adulterated) foundations were placed in the colonies, one for each adulterant and percentage. The workers were marked upon emergence from these combs and those bees were introduced into one strong colony per adulterant and percentage. Phenoloxidase and lysozyme activities were determined in the haemolymph of 1-, 7- and 14-day-old workers.</p><p><strong>Results: </strong>The higher the concentrations of stearin and paraffin in the wax foundation, the lower the phenoloxidase activities were. These activities increased with the bee age. In contrast, the trends in lysozymes were opposite. Paraffin seems to be less toxic than stearin.</p><p><strong>Conclusion: </strong>Adulteration of wax foundation with even a small amount of stearin or paraffin has negative effects on the functioning of the bee.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of central administration of indomethacin on anandamide-induced GnRH/LH secretion in the hypothalamus of anoestrous ewes","authors":"D. Tomaszewska-Zaremba, M. Tomczyk, Karolina Wojtulewicz, J. Bochenek, K. Pałatyńska, A. P. Herman","doi":"10.2478/jvetres-2024-0039","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0039","url":null,"abstract":"\u0000 \u0000 \u0000 It is suggested that cannabinoids (CBs) may disturb reproduction through action on hypothalamic gonadotropin-releasing hormone (GnRH) neurons directly or indirectly through intermediates such as prostaglandins. The study aimed to determine the influence of intracerebroventricular (i.c.v.) injection of the endogenous cannabinoid anandamide (N-arachidonoylethanolamine – AEA), alone or with the prostaglandin synthesis inhibitor indomethacin (IND), on GnRH/luteinising hormone (LH) secretion. The purpose of the research was to clarify the role of endocannabinoids and their interaction with prostaglandins in the regulation of reproduction at the level of the hypothalamus and pituitary in anoestrous sheep.\u0000 \u0000 \u0000 \u0000 The study was performed on 24 anoestrous ewes divided into four experimental groups: a control group receiving i.c.v. injection of Ringer–Locke solution, an AEA group that received i.c.v. injection of 30 μM of AEA, an IND group receiving i.c.v. injection of 5 μM of IND and an AEA + IND group that received i.c.v. injections of 30 μM of AEA and 5 μM of IND.\u0000 \u0000 \u0000 \u0000 Anandamide stimulated GnRH protein and gene expression in the median eminence and protein expression in the preoptic area without influencing GnRH messenger RNA (mRNA) in this structure. Indomethacin reversed the changes in GnRH secretion after AEA administration. It was also found that AEA stimulated LH mRNA in the pituitary without influencing LH release.\u0000 \u0000 \u0000 \u0000 Our results support the role of endogenous cannabinoids in the regulation of reproductive processes at the central nervous system level. They may act directly on the hypothalamic GnRH neurons or indirectly through intermediates such as prostaglandins.\u0000","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141804149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of β-hydroxybutyrate acid on gene expression levels of antioxidant biomarkers and growth hormone-related genes in liver cell culture.","authors":"Muhammad Ali Mohsin, Xiaojing Zhou, Yu Huiru, Wenxiang Shen, Baoxiang He, Przemysław Sobiech, Mariusz Pierzchała, Magdalena Ogłuszka, Rafał Starzyński, Garima Kalra, Bharti Deshmukh, Revathy Thangarasu, Neeraj Kashyap, Urszula Czarnik, Adam Lepczyński, Grzegorz Woźniakowski, Chandra S Pareek","doi":"10.2478/jvetres-2024-0037","DOIUrl":"10.2478/jvetres-2024-0037","url":null,"abstract":"<p><strong>Introduction: </strong>In dairy cattle, oxidative stress is a predominant problem associated with diseases and reproductive health issues. This study aimed to detect the variation in the antioxidant biomarkers by adding different concentrations of β-hydroxybutyric acid (BHBA) and sought to elucidate its effects on the gene expression levels of growth hormone (GH) and antioxidant biomarkers in bovine hepatocytes.</p><p><strong>Material and methods: </strong>Four antioxidant biomarkers, namely malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH Px) were evaluated using commercially available bovine ELISA kits. The expression levels of the bovine GH, its receptor (GHR), insulin-like growth factor (IGF), IGF-1, IGF-1 receptor, CAT, SOD, GSH-Px and β-actin (as a reference) genes in liver cell culture were determined by reverse transcriptase-PCR assay.</p><p><strong>Results: </strong>With the increase of BHBA concentration and culture time, the activities of SOD, CAT, and GSH Px biomarkers in hepatocytes decreased. However, the content of MDA in hepatocytes increased gradually with the increase of hepatocyte culture time and BHBA concentration. The qPCR results revealed that after adding BHBA, gene expression levels of GSH-Px, SOD and IGF biomarkers in hepatocytes began to differ in the culture groups at 12 h, whereas the gene expression level of the CAT and GHR biomarkers in hepatocytes began to differ at 6 h.</p><p><strong>Conclusion: </strong>Quantitative PCR results showed that the BHBA significantly downregulated the expression levels of the GHR gene and CAT, GSH Px and SOD antioxidant biomarker genes.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation and application of a method for determination of multi-class pesticides in muscle chicken breast fillets using QuEChERS extraction and GC/MS.","authors":"Aleksandra Mihail Tasic, Milan Ninković, Ivan Pavlović","doi":"10.2478/jvetres-2024-0036","DOIUrl":"10.2478/jvetres-2024-0036","url":null,"abstract":"<p><strong>Introduction: </strong>The occurrence of pesticide residues in animal products deserves attention because of the contamination by environmental pollutants and pesticides that may be present in the food that animals are fed. The goal of this work was the validation of a method for detection of residues of multiple classes of pesticide and determination of their residues in chicken breast fillets.</p><p><strong>Material and methods: </strong>Gas chromatography with mass spectrometry was used for analysis. A modified quick, easy, cheap, effective, rugged and safe (QuEChERS) method was put into practice for its validation and applied to real samples. The study optimised mass detection and investigated the effect of a freezing step during the preparation of samples. Pesticides were determined in samples from conventional and organic production.</p><p><strong>Results: </strong>The impact of the matrix effect decreased, with the largest number of pesticides and satisfactory recovery determined by the application of mixed solvent acetonitrile and ethyl acetate for extraction. Detection of pesticide residues was achieved in a linear range between 5 and 50 µg/kg with satisfactory excellent correlation coefficients greater than 0.99. The recovery of all the pesticide residues ranged between 71.2 and 118.80%. The relative standard deviation was from 2.9% to 18.1% for all validated pesticide residues. The limits of quantification were in the range of 3.0-4.9 µg/kg. Out of 56 pesticide residues analysed in real samples, 5 were detected: α endosulfan, cypermethrin, endosulfan sulphate, permethrin and <i>p</i>,<i>p´</i>-dichlorodiphenyltrichloroethane (DDT) and their concentrations ranged from 4.9 to 15.2 µg/kg.</p><p><strong>Conclusion: </strong>All tested samples were compliant with the evaluation criteria, and detected values of pesticide residues were lower than the maximum residual levels.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the rabies virus variants circulating in Poland in 2021-2023 and their phylogeny with analysis of the strains in the Mazowieckie and Podkarpackie voivodeships.","authors":"Janusz Ciołek, Anna Orłowska, Marcin Smreczak","doi":"10.2478/jvetres-2024-0032","DOIUrl":"10.2478/jvetres-2024-0032","url":null,"abstract":"<p><strong>Introduction: </strong>Rabies is endemic in Europe and red foxes are the vector and reservoir of the rabies virus (RABV). Based on classification established in the early 1990s, four variants of the rabies virus have been distinguished in Europe. Rabies broke out in January 2021 in the Mazowieckie voivodeship in central north-eastern Poland. The virus spread rapidly, reaching the Świętokrzyskie voivodeship in the central southern part and the Lubelskie voivodeship in the eastern part in the next months. Nine rabies cases were reported in the Podkarpackie voivodeship in south-eastern Poland between 2021 and 2023, mainly in red foxes but also in dogs and wildcat. The aim of the study was the identification of RABV variants in wildlife and domestic animals in Poland between 2021 and 2023.</p><p><strong>Material and methods: </strong>The study involved 157 animal brains tested positive for rabies using a fluorescent antibody test. From 10% w/v brain homogenates, RNA was isolated and full-length RABV genomes were high-throughput sequenced with an RABV-enriched approach. Complete genomes of RABV isolates were phylogenetically analysed and the variants were estimated.</p><p><strong>Results: </strong>Molecular and phylogenetic studies revealed 147 (93.6%) of the RABV strains out of 157 which had rapidly spread in the wildlife of the Mazowieckie, Świętokrzyskie and Lubelskie voivodeships to be Central European strains. Nine RABVs (5.7%) detected in foxes, a wildcat and a dog in the Podkarpackie voivodeship were identified as North-Eastern European. A vaccine-induced rabies case was detected in a red fox in the Lubelskie voivodeship in May 2023.</p><p><strong>Conclusion: </strong>Central European and North-Eastern European RABVs were circulating in Poland between 2021 and 2023.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11212032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}