Journal of Veterinary Research最新文献

{"title":"Evaluation of the utility of testicular-only processing fluid for porcine reproductive and respiratory syndrome virus diagnostics and the effect of sample pooling on the test results.","authors":"Hanna Turlewicz-Podbielska, Arkadiusz Dors, Małgorzata Pomorska-Mól","doi":"10.2478/jvetres-2025-0011","DOIUrl":"10.2478/jvetres-2025-0011","url":null,"abstract":"<p><strong>Introduction: </strong>The testicular-only processing fluid (TOPF) obtained from piglet testicles after castration could be an alternative sample for porcine reproductive and respiratory syndrome (PRRS) laboratory diagnosis. If this matrix were proved useful, testing it would spare piglets the stress of blood drawing and eliminate some labour required to take blood samples. The aim of the study was to evaluate the utility of TOPF for this diagnostic purpose.</p><p><strong>Material and methods: </strong>Serum-and-TOPF pairs from male piglets and sera from female piglets were tested using commercial ELISA and real-time RT-PCR kits. For the pooling simulation, 10 μL aliquots of TOPF separated into low-, moderately and highly positive were mixed with appropriate volumes of negative TOPF samples. This simulated pools of 5, 10, 20, 40 and 80 samples containing 1 positive for serological analyses and pools of 10, 20, 40, 80, 160 and 320 samples containing 1 positive in molecular analyses.</p><p><strong>Results: </strong>The percentages of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies were statistically significantly different (P-value < 0.05) between boar sera (69.55%) and TOPF (54.49%), as well as between gilt sera (74.52%) and TOPF. However, after adjusting the cut-off value, no significant differences were noted. The RNA of PRRSV was detected in 21.26% of male sera, 15.23% of TOPFs and 17.00% of female sera. Pooled sample testing revealed discrepancies in positive results associated with the pool size and original sample positivity strength.</p><p><strong>Conclusion: </strong>TOPF samples can be a valuable matrix for laboratory PRRS diagnosis in piglets. However, it is important to be aware of the potential for false-negative results.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"7-16"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The course of pigeon circovirus infection in young pigeons experimentally kept under conditions mimicking the One Loft Race rearing system.","authors":"Daria Dziewulska, Bartłomiej Tykałowski, Ewa Łukaszuk, Tomasz Stenzel","doi":"10.2478/jvetres-2025-0009","DOIUrl":"10.2478/jvetres-2025-0009","url":null,"abstract":"<p><strong>Introduction: </strong>Racing pigeon competitions are a popular sport where success depends on birds' ability to return fast to their loft of origin. However, many additional factors like differences in feeding, training, everyday care and even geographical loft location influence race outcomes, which has led to the development of the One Loft Race (OLR) system. The OLR system aims to eliminate these factors by housing pigeons from various lofts in equal conditions in one facility. This in turn, however, fosters inter-individual transmission of pathogens.</p><p><strong>Material and methods: </strong>Fifteen young racing pigeons from five different lofts, naturally infected with pigeon circovirus (PiCV) were reared in one unit for six weeks. Four uninfected birds were kept in a separate unit and were treated as controls for flow cytometry analyses (background establishment). Blood samples were collected every seven days to extract DNA for PiCV quantification using droplet digital PCR and to isolate the mononuclear cells for flow cytometry analyses. On day 42, all birds were euthanised for spleen samples to be collected for further analyses.</p><p><strong>Results: </strong>The viraemia peak was noted on day 14 of the experiment and subsequently decreased afterwards, with a remarkable decrease noted on day 35. The percentage of IgM⁺ B lymphocytes, including apoptotic cells, in the blood was very similar throughout the experiment. The percentage of apoptotic splenic IgM⁺ B cells was approximately 40% higher in the experimental group than in the control group.</p><p><strong>Conclusion: </strong>Study results showed that the birds' adaptation period and the specific immunity they had probably developed hindered PiCV replication. Mild PiCV infection led to a slight increase of B lymphocyte apoptosis in the spleen.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"1-6"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic insights into nematode-trapping fungi <i>Arthrobotrys oligospora</i> after their response to chitin.","authors":"Jiahua Zhang, Lixiang Wei, Huimei Zhang, Xixi Ma, Yansen Sun, Ruobing Li, Chengzhi Zhang, Xuepeng Cai, Jun Qiao, Qingling Meng","doi":"10.2478/jvetres-2025-0005","DOIUrl":"10.2478/jvetres-2025-0005","url":null,"abstract":"<p><strong>Introduction: </strong>Nematode-trapping fungi (NTFs) can produce various chitinases to degrade nematode body wall and eggshell chitin during predation. However, the regulatory mechanisms of their expression of chitinases still remain unclear. The primary objective of this study was to elucidate the differential protein profile of <i>A. oligospora</i>, an NTF, in response to chitin.</p><p><strong>Material and methods: </strong>Colloidal chitin was added to induce the culture of <i>A. oligospora</i>, and the phenotypic differences before and after induction were observed under inverted microscope. The differential proteins before and after mycelium induction were screened by liquid chromatography-tandem mass spectrometry. The differentially expressed chitinase was expressed in <i>Pichia</i> yeast, and the recombinant enzyme was incubated with <i>Caenorhabditis elegans</i> and its egg suspension to explore its biological activity.</p><p><strong>Results: </strong>It was found that there was a significant acceleration in the mycelial growth post chitin interaction in <i>A. oligospora</i>. A total of 1,124 differentially expressed proteins (DEPs) were identified between the control group (AO-c) and the experimental group (AO-e), with 183 upregulated and 941 downregulated. Gene Ontology analysis revealed that the DEPs acted in various metabolic processes with catalysis and binding functions. Kyoto Encyclopedia of Genes and Genomes analysis associated these proteins primarily with signalling pathways related to glucose metabolism. Three chitinases were significantly modulated among DEPs. Moreover, enzymatic activity assays demonstrated that one of them effectively degraded <i>C. elegans</i> and its eggs.</p><p><strong>Conclusion: </strong>These findings suggest that <i>A. oligospora</i> can significantly alter its protein expression profile in response to chitin, thereby facilitating its sugar metabolism and mycelial development. Our study provided new insights into the regulatory mechanisms of nematode predation in <i>A. oligospora</i>.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"71-82"},"PeriodicalIF":1.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is <i>Pseudomonas aeruginosa</i> a possible aetiological agent of periodontitis in dogs?","authors":"Małgorzata Targońska-Karasek, Izabela Polkowska, Henryk Krukowski","doi":"10.2478/jvetres-2025-0006","DOIUrl":"10.2478/jvetres-2025-0006","url":null,"abstract":"<p><strong>Introduction: </strong>Periodontal diseases are the most frequently diagnosed problem in small animal veterinary medicine. Although their exact cause is not fully understood, bacteria play an important role in their development. <i>Pseudomonas aeruginosa</i> is a Gram-negative, rod-shaped, non-spore-forming bacterium. The living environment of this bacterium may be soil and water; however, it can also be found in humans and animals. Antibiotic treatment of periodontitis may be complicated by the carbapenem resistance of some <i>P. aeruginosa</i> strains, if these bacteria are found to be an aetiological agent. The aim of the study was to identify all bacterial strains isolated from dog with periodontitis.</p><p><strong>Material and methods: </strong>After a clinical examination of a Schnauzer dog in the Department and Clinic of Animal Surgery in the University of Life Sciences in Lublin Faculty of Veterinary Medicine, periodontitis was diagnosed. A swab was taken from the diseased tissue and submitted for microbiological tests. Microorganisms were initially identified by colony morphology, haemolytic pattern and Gram staining, and subsequently by sensitivity tests, VITEK 2 and matrix-assisted laser desorption/ionisation-time-of-flight.</p><p><strong>Results: </strong><i>Pseudomonas aeruginosa</i> was isolated and identified as a probable aetiological factor of periodontitis in dogs.</p><p><strong>Conclusion: </strong>In our opinion, attention should be paid to <i>Pseudomonas aeruginosa</i> as a possible aetiological factor of periodontal diseases in dogs.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"35-40"},"PeriodicalIF":1.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of body condition score loss during the transition period on metabolism, milk yield and health in Holstein cows.","authors":"Rui Sun, Xuejie Jiang, Yu Hao, Ying Li, Yunlong Bai, Cheng Xia, Yuxi Song","doi":"10.2478/jvetres-2025-0004","DOIUrl":"10.2478/jvetres-2025-0004","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to investigate the impact of perinatal body condition score (BCS) and its subsequent loss on postpartum performance and health outcomes in dairy cattle.</p><p><strong>Material and methods: </strong>A total of 156 cows were randomly selected, and blood samples were collected at -21, 0, 7, 14, 21, 28 and 50 days relative to calving. Milk yield and disease incidence in dairy cows were recorded after calving. These cows were subsequently categorised into three groups based on BCS loss during the transition period: a no-BCS-loss (maintained BCS) group (M, 0 < BCS loss ≤ 0.25), low-BCS-loss group (L, 0.25 < BCS loss ≤ 0.5), and high-BCS-loss group (H, BCS loss > 0.5).</p><p><strong>Results: </strong>All groups experienced a decline in BCS from 21 days prepartum through 50 days postpartum (P-value < 0.01). Cows in the H group had the highest levels of non-esterified fatty acids, beta-hydroxybutyrate, total cholesterol, aspartate aminotransferase, albumin, malondialdehyde and leptin (P-value < 0.05). Concomitantly, total antioxidant capacity, as well as the levels of insulin and glucose, were the lowest in group H (P-value < 0.05). Plasma concentrations of Ca, P, Mg and K, urea nitrogen and total bilirubin were not significantly influenced by BCS loss (P-value > 0.05). Cows in the M group were less likely to develop ketosis, mastitis, retained placenta, displaced abomasum and metritis than those in the H group, and cows in the H group produced the lowest milk yields (P-value < 0.05).</p><p><strong>Conclusion: </strong>These observations collectively indicate that BCS loss is associated with measurable changes in energy balance, liver function, oxidative stress, daily milk production and disease incidence during the transition period.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"91-99"},"PeriodicalIF":1.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remodelling of the healthy foal's conjunctival microbiome in the first two months of life.","authors":"Katarzyna Płoneczka-Janeczko, Eve Armstrong, Marta Siemieniuch-Tartanus, Marcin Magdziarz","doi":"10.2478/jvetres-2025-0001","DOIUrl":"10.2478/jvetres-2025-0001","url":null,"abstract":"<p><strong>Introduction: </strong>The aim of the study was to explore and characterise healthy foals' eye microbiomes in the first two months of life.</p><p><strong>Material and methods: </strong>Conjunctival swabs were collected three times, not later than 12 h after delivery and again at the end of the first and the second months of life from six clinically healthy foals of the Polish Konik breed. The average interval between the first and second samplings was 33.3 days and between the second and third was 35.6 days. Next-generation sequencing performed on a MiSeq sequencer in paired-end technology was used to analyse the composition of the conjunctival microbiota.</p><p><strong>Results: </strong>Paired one-sided <i>t</i>-tests revealed that conjunctival microbiota diversity was the lowest in the first 24 h of life and significantly increased between birth and the first month. The most prevalent family throughout the study was <i>Micrococcaceae</i> and the most prevalent genus was <i>Corynebacterium</i>. Sequences of potentially pathogenic bacteria such as <i>Pseudomonas, Acinetobacter</i> and <i>Streptococcus</i> spp. that may be involved in inflammatory processes were identified. Ocular commensals such as <i>Corynebacterium</i> and <i>Lactobacillaceae</i> that were found in the ocular surface microbiome of the foals are believed to be capable of restoring the ocular microbiome and maintaining balance.</p><p><strong>Conclusion: </strong>A healthy ocular surface microbiota in the early period of a foal's life develops dynamically and changes its composition.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"131-140"},"PeriodicalIF":1.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of neurokinin B, dynorphin A and kisspeptin-10 on <i>in vitro</i> gonadotropin secretion by anterior pituitary cells isolated from pubescent ewes.","authors":"Natalia Szysiak, Urszula Kosior-Korzecka, Vincenzo Longo, Krzysztof Patkowski, Monika Greguła-Kania, Aneta Nowakiewicz, Mariola Bochniarz, Andrzej Junkuszew","doi":"10.2478/jvetres-2025-0003","DOIUrl":"10.2478/jvetres-2025-0003","url":null,"abstract":"<p><strong>Introduction: </strong>The hypothalamic neuropeptides kisspeptin-10 (KiSS-10), neurokinin B (NKB), and dynorphin A (Dyn A) play roles in the endocrine regulation of the hypothalamic-pituitary-ovarian (HPO) axis in puberty. Livestock's timely attainment of sexual maturity increases reproductive efficiency and raises profitability. The pituitary relationship between these neuropeptides and gonadotropins in puberty in ewes being undercharacterised. The aim of the study was to analyse their direct effect on gonadotropin secretion by pituitary cells isolated from pubescent ewes.</p><p><strong>Material and methods: </strong>Cells were incubated in McCoy's 5A medium, either without neuropeptides (as the control) or with 10^-11, 10^-10, 10^-9, 10^-8 and 10^-7 M of KiSS-10, NKB and Dyn A. After 4, 12 and 24 h, the luteinising hormone (LH) and follicle-stimulating hormone (FSH) concentrations were analysed by ELISA using species-specific antibodies.</p><p><strong>Results: </strong>Greater LH and FSH secretion was observed after the 4-24 h exposure to respective 10^-11-10^-8 M and 10^-11-10^-7 M concentrations of KiSS-10. Moreover, NKB and Dyn A applied in the concentration range elevated the secretion of both LH and FSH throughout the experiment. Dynorphin A had the most significant effect on gonadotropin secretion at all the concentrations used. In contrast, the most pronounced dose-dependent neuropeptide effect throughout the experiment on the FSH secretion was attributed to NKB.</p><p><strong>Conclusion: </strong>Kisspeptin-10, NKB and Dyn A had a direct impact on gonadotropin secretion by ovine pituitary cells. However, a detailed explanation of their role in gonadotropin secretion by the anterior pituitary gland in sheep and of their impact on the regulation of the HPO axis during sexual maturation or in the pathomechanism of delayed puberty require further studies.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"121-129"},"PeriodicalIF":1.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution, lipophilicity and tissue half-life as key factors in sulphonamide clearance from porcine tissues.","authors":"Artur Burmańczuk, Monika Osypiuk, Bożena Polska, Dominik Kunicki, Marcin Kocik, Karol Grzęda, Włodzimierz Markiewicz, Oktay Yilmaz, Tomasz Grabowski","doi":"10.2478/jvetres-2025-0002","DOIUrl":"10.2478/jvetres-2025-0002","url":null,"abstract":"<p><strong>Introduction: </strong>Sulphonamides are some of the most widely used antimicrobial drugs in the treatment of bacterial diseases in pigs. The study was conducted to compare the total exposure of tissue to a group of active substances in a single formulation and evaluate the impact of the volume of distribution, lipophilicity and tissue half-life of sulfadimethoxine, sulphathiazole, sulphamethazine and sulphacetamide in two different veterinary drug formulations - Polisulfalent, which was preparation A, and Polisulfamid, which was preparation B.</p><p><strong>Material and methods: </strong>Each tested therapeutic preparation was administered to 15 piglets of the Polish Landrace breed. To assume general exposure expressed as the sum of all observed concentrations of all active substances, semi-log regression analyses were performed for both formulations.</p><p><strong>Results: </strong>The estimated tissue half-life was 21.19 h for preparation A and 17.36 h for preparation B. The comparison of the semi-log regression parameters shows that formulation B's Y-intercept and slope values were higher than formulation A's. The upper-bound CI for the Y-intercept and slope value of formulation B was higher than those of formulation A, at 38.7 and 20.8%, respectively.The lower-bound CI for the Y-intercept and slope value of formulation B was also higher than those of formulation A, at 20.6 and 42.9%, respectively.</p><p><strong>Conclusion: </strong>Current observations lead to the conclusion that in late-stage drug elimination, it is not the blood half-life, but rather a drug's volume of distribution which is key in determining the clearance period, this volume being dependent on the physicochemical characteristics of the drug.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"101-109"},"PeriodicalIF":1.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of chlamydiae in dairy cattle herds and factors contributing to the spread of infections.","authors":"Mońika Szymańska-Czerwińska, Agnieszka Jodełko, Zbigniew Osiński, Kinga Zaręba-Marchewka, Krzysztof Niemczuk","doi":"10.2478/jvetres-2024-0071","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0071","url":null,"abstract":"<p><strong>Introduction: </strong>Different <i>Chlamydia</i> species affect cattle and contribute to economic losses. One of them, <i>C. pecorum</i>, is a globally endemic livestock pathogen. Despite its endemicity, prevalence data from Poland have so far been limited. The present study aimed to obtain insight into the chlamydiae prevalence in Polish dairy cattle.</p><p><strong>Material and methods: </strong>A screening of chlamydial seroprevalence in dairy cattle was initially performed, followed by <i>Chlamydiaceae</i>- and species-specific real-time qPCR. Vaginal swabs (n = 239) and placenta samples (n = 2) from seropositive animals in 142 herds were collected to detect shedders. The study population consisted of cows (n = 2,780) from dairy herds (n = 1,153) located in all Polish voivodeships.</p><p><strong>Results: </strong>The true animal prevalence was determined to be 33.3%, while the true herd prevalence was 42.7%. Five groups of Polish voivodeships were identified using appropriate statistical tools, highlighting differences that may arise from various factors impacting the spread of chlamydial infections. The only detected chlamydia species was <i>C. pecorum</i>, the presence of which was confirmed in two herds.</p><p><strong>Conclusion: </strong>This study revealed that chlamydial infections are commonly present in Polish dairy cattle across the country.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"531-538"},"PeriodicalIF":1.3,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of clostebol residues in the urine of slaughter animals using liquid chromatography-tandem mass spectrometry.","authors":"Iwona Matraszek-Żuchowska, Alicja Kłopot, Justyna Grzelak, Paulina Zdonek","doi":"10.2478/jvetres-2024-0070","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0070","url":null,"abstract":"<p><strong>Introduction: </strong>Synthetic anabolic hormones, which may pose a potential risk to human health, should not be used in fattening food-producing animals. Because of the hormonal effects they cause, growth-promoting compounds are banned by legislation in the EU for use in animal husbandry. Consequently, all EU member states are required to conduct monitoring tests on the content and residues of these compounds in prescribed biological matrices to ensure the safety of food consumers. The aim of this research was to develop a liquid chromatography-tandem mass spectrometry method for the detection of the residue of one such anabolic hormone, clostebol in food animal urine.</p><p><strong>Material and methods: </strong>Clostebol and its marker metabolite residues were determined by a method involving enzymatic hydrolysis, isolation of compounds from urine on a C18 solid-phase extraction (SPE) column, purification of the extract by liquid-liquid extraction using n-pentane and a NH₂ SPE column, and detection by liquid chromatography-tandem mass spectrometry.</p><p><strong>Results: </strong>No traces of this anabolic steroid hormone or its metabolites were found in any of the samples tested. The method was validated in accordance with the current requirements for confirmatory methods, and the determined parameters of the decision limits necessary for assessing sample compliance met the specified criteria.</p><p><strong>Conclusion: </strong>In 2023, the method was introduced for testing under the National Control Plan in Poland. Up to July 19, 2024, 53 urine samples from different animal species had been tested.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"611-621"},"PeriodicalIF":1.3,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}