Journal of Veterinary Research最新文献

{"title":"Effect of neurokinin B and dynorphin A on kisspeptin-10 secretion from the anterior pituitary cells of pubescent ewes <i>in vitro</i>.","authors":"Natalia Szysiak, Urszula Kosior-Korzecka, Monika Greguła-Kania, Krzysztof Patkowski, Mateusz Fila, Andrzej Junkuszew","doi":"10.2478/jvetres-2025-0026","DOIUrl":"10.2478/jvetres-2025-0026","url":null,"abstract":"<p><strong>Introduction: </strong>Neurokinin B (NKB), dynorphin A (Dyn A) and kisspeptin (KiSS) are key agents that participate in the neuroendocrine regulation of the development and functioning of the reproductive system. While the role of KiSS is better understood, the functions of NKB and Dyn A at the pituitary level have not been elucidated. The objective of our study was to analyse their direct effect on kisspeptin-10 (KiSS-10) secretion by anterior pituitary cells isolated from pubescent ewes.</p><p><strong>Material and methods: </strong>Pituitary cells from 10-month-old ewe lambs were incubated in McCoy's 5A medium without hormones (the control), or with 10^-11, 10^-10, 10^-9, 10^-8 or 10^-7 M of NKB or Dyn A for 2, 4, 6, 12, 18 or 24 h. The concentration of KiSS-10 was analysed by ELISA using species-specific antibodies.</p><p><strong>Results: </strong>When applied at the concentrations of 10^-10-10^-7 M, NKB increased KiSS-10 secretion throughout the entire experiment (2-24 h), compared to the control. Significantly higher (P-value ≤ 0.05) KiSS-10 release than in the control was observed after 6-24 h exposure of the cells to 10^-8 M of NKB. However, no effect of NKB on the secretion of KiSS-10 was shown when applied at the lowest concentration (10^-11 M). In turn, there was no significant effect of Dyn A at any concentration on KiSS-10 secretion by pituitary cells at any time.</p><p><strong>Conclusion: </strong>In contrast to Dyn A, NKB can directly affect KiSS-10 secretion from the pituitary cells of pubescent ewes in a way dependent on the time of exposure to this neuropeptide and its concentration in the culture medium. This phenomenon may indicate a potential role of NKB in the initiation of reproductive activity, which leads to the achievement of sexual maturity in the optimal developmental window.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 2","pages":"299-304"},"PeriodicalIF":1.3,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical coding of piglets small intestine neurons after prenatal exposure to β-hydroxy-β-methylbutyrate.","authors":"Aleksandra Dajnowska, Katarzyna Kras, Ewa Tomaszewska, Piotr Dobrowolski, Renata Klebaniuk, Siemowit Muszyński, Marcin Bartłomiej Arciszewski","doi":"10.2478/jvetres-2025-0024","DOIUrl":"10.2478/jvetres-2025-0024","url":null,"abstract":"<p><strong>Introduction: </strong>The global swine industry faces significant challenges related to improving the survival and health of newborn piglets. Attention has come to β-hydroxy-β-methylbutyrate (HMB), a metabolite of leucine, for its potential in prenatal nutritional programming in sows, which can improve piglet body weight and support the development of the skeletal and digestive systems. The effects of prenatal HMB supplementation were investigated on the chemical coding of the enteric nervous system (ENS) in the small intestine of neonatal piglets.</p><p><strong>Material and methods: </strong>The experiment was conducted on piglets from 12 sows divided into a control and an experimental group. Sows in the experimental group received HMB at a dose of 0.2 g/kg body weight per day from day 70 to 90 of gestation. After parturition, one piglet from each litter was euthanised and parts of the duodenum, jejunum and ileum were exsected. Tissue sections were fixed in paraffin, reacted with anti-vasoactive intestinal peptide (VIP), anti-cocaine- and-amphetamine-regulated transcript (CART), anti-neuronal nitric oxide synthase (nNOS) and anti-substance P (SP) antibodies, and the immunoexpression of VIP, CART, nNOS and SP was determined histomorphometrically by calculating the area of fibres which were immunoreactive with each.</p><p><strong>Results: </strong>Supplementation with HMB in sows caused significant changes in the ENS of newborn piglets, including an increase in the area of fibres reactive to CART and nNOS in certain layers and sections of the small intestine, and a decrease in the area of fibres reactive to SP and VIP.</p><p><strong>Conclusion: </strong>The results indicate that prenatal supplementation with HMB in sows may significantly influence the functioning of the gastrointestinal tract in newborn piglets.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 2","pages":"249-255"},"PeriodicalIF":1.3,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vivo</i> efficacy of macrocyclic lactones on goat farms - pour-on <i>vs</i> injectable application.","authors":"Michal Babják, Alžbeta Königová, Tetiana A Kuzmina, Marián Várady","doi":"10.2478/jvetres-2025-0025","DOIUrl":"10.2478/jvetres-2025-0025","url":null,"abstract":"<p><strong>Introduction: </strong>Pour-on eprinomectin (EPN) is the only anthelmintic currently available that is approved for use in lactating goats with a zero-day milk withdrawal period. The aim of this study was to compare the efficacy of the pour-on form of EPN with the efficacy of the injectable version of ivermectin (IVM) on goat farms in Slovakia.</p><p><strong>Material and methods: </strong>The study was conducted from September to December 2023 on eight dairy-goat farms. The goats on six farms were divided into two groups of 10 animals each. The first group was administered pour-on EPN at the recommended dose for sheep and goats, and the second group was treated with injectable IVM at a dose 1.5-fold higher than the recommended dose for sheep. The goats on the remaining two farms were treated only with EPN. Efficacy was determined using the <i>in vivo</i> faecal egg count reduction test and the <i>in vitro</i> larval development test (LDT). Infectious third-stage (L3) larvae were harvested before and 14 days after treatment and were assigned to species/genus based on morphology.</p><p><strong>Results: </strong>The percent reductions for IVM and EPN ranged from 80.8 to 93.6 and 51.0 to 96.6, respectively. A substantially higher egg reduction on the 14^th day post treatment of 20 to 30% was noted in the groups on three farms treated with injectable IVM. The reduction differed little between the drugs on the other three farms (1-3%). Infectious L3 larvae in the LDT were observed at concentrations equal to or higher than the minimum inhibitory concentration of 21.6 ng/mL on all farms. <i>Haemonchus contortus</i> dominated on the 14^th day post treatment with both drugs on all farms.</p><p><strong>Conclusion: </strong>This study is the first to report reduced efficacy of the pour-on form of EPN in goat herds in Slovakia.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 2","pages":"293-298"},"PeriodicalIF":1.3,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of <i>Fasciola hepatica</i> seroprevalence and risk factors in European bison <i>(Bison bonasus)</i>.","authors":"Anna Didkowska, Daniel Klich, Katarzyna Filip-Hutsch, Katarzyna Matusik, Monika Krajewska-Wędzina, Marlena Wojciechowska, Stanisław Kaczor, Wanda Olech, Krzysztof Anusz","doi":"10.2478/jvetres-2025-0020","DOIUrl":"10.2478/jvetres-2025-0020","url":null,"abstract":"<p><strong>Introduction: </strong><i>Fasciola hepatica</i> is a trematode that infects ruminants worldwide. It is also the infectious agent of a zoonosis, fasciolosis, which is considered to be a re-emerging disease. There is no data about <i>F. hepatica</i> seroprevalence in European bison (<i>Bison bonasus</i>); however, complex population health monitoring is particularly important in protected species such as this. Addressing the need for this surveillance, the aim of this study was to assess for the first time the seroprevalence of <i>F. hepatica</i> in Polish free-living European bison populations and identify risk factors for infection.</p><p><strong>Material and methods: </strong>Between 2020 and 2024, serum samples were collected from 119 free-ranging European bison from mountains and lowland areas. Serum samples were tested with a commercial ELISA to detect antibodies to <i>Fasciola hepatica</i>, and the data yielded were statistically analysed.</p><p><strong>Results: </strong>The study revealed <i>F. hepatica</i> seropositivity in 20/119 animals (16.8%), with higher herd seroprevalence in lowland groups, and higher individual seroprevalence in females and animals from lowland areas.</p><p><strong>Conclusion: </strong>Our study shows that a serological examination may be a useful and convenient diagnostic tool in assessing the <i>F. hepatica</i> exposure of the European bison population, especially when performing epizootic and retrospective studies.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 2","pages":"227-232"},"PeriodicalIF":1.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of sheep exposure to poly- and perfluoroalkyl substances through hair sample analysis.","authors":"Sławomir Gonkowski, Julia Martín, Nariste Kadyralieva, Irene Aparicio, Juan Luis Santos, Esteban Alonso","doi":"10.2478/jvetres-2025-0023","DOIUrl":"10.2478/jvetres-2025-0023","url":null,"abstract":"<p><strong>Introduction: </strong>Poly- and perfluoroalkyl substances (PFASs) are a numerous group of synthetic organic compounds used in various industries. They pollute the natural environment and negatively affect humans and animals. The aim of the present investigation was to assess the exposure of sheep bred in the Kyrgyz Republic to six selected PFASs.</p><p><strong>Material and methods: </strong>Six selected PFASs were assessed in 50 sheep (39 females and 11 males) of Kyrgyz and Arashan breeds ranging in age from 1 to 8 years (mean 2.1 ± 1.1), recruited equally from the Alamedin and Sokuluk regions. The substances were five perfluoroalkyl carboxylic acids (perfluorobutanoic acid - PFBuA, perfluoropentanoic acid - PFPeA, perfluorohexanoic acid - PFHxA, perfluoroheptanoic acid - PFHpA and perfluorooctanoic acid - PFOA) and perfluorooctane sulphonic acid (PFOS), and were determined through the liquid chromatography-tandem mass spectrometry of hair samples.</p><p><strong>Results: </strong>All the listed PFASs were found in the studied hair samples. The highest concentrations were noted for PFPeA and PFBuA. The concentration of PFPeA ranged from 0.99 ng/g to 27.90 ng/g (mean 5.55 ± 4.54 ng/g) and that of PFBuA from 0.95ng/g to 14.18 ng/g (mean 2.24 ± 2.34 ng/g). The mean concentration levels of other PFASs were as follows: 1.06 ± 0.78 ng/g for PFHxA, 1.02 ± 0.76 ng/g for PFHpA, 0.87 ± 0.68 ng/g for PFOA and below the method quantification limit for PFOS. Clear differences in PFASs levels were noted between the two regions.</p><p><strong>Conclusion: </strong>Sheep are exposed to various PFASs, and sheep wool and items made of it may be the source of human exposure to these compounds. Hair samples may be used for biomonitoring of sheep exposure to PFASs.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 2","pages":"285-292"},"PeriodicalIF":1.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of herbal feed additives on milk performance and health status of dairy goats.","authors":"Jacek Antoni Wójtowski, Jan Pikul, Przemysław Mikołajczak, Michał Czopowicz, Jarosław Kaba, Joanna Foksowicz-Flaczyk, Ireneusz Antkowiak, Jarosław Pytlewski, Maria Markiewicz-Kęszycka, Daniel Stanisławski","doi":"10.2478/jvetres-2025-0021","DOIUrl":"10.2478/jvetres-2025-0021","url":null,"abstract":"<p><strong>Introduction: </strong>Interest is increasing in natural feed additives that improve animal health, raise farming productivity and enhance the quality of animal products. These additives, especially polyphenols, are biologically active chemical compounds found in plants.</p><p><strong>Material and methods: </strong>Sixty dairy goats were randomly assigned to five feeding groups of 12 animals each. Over 16 weeks, the animals received a polyherbal supplement containing seven or nine herb species at 20 or 40 g/animal/day, along with pelleted concentrate feed. The health status of the animals was assessed based on the concentration of acute phase haptoglobin proteins and serum amyloid A in blood serum.</p><p><strong>Results: </strong>A statistically significant positive effect of the herbal mixtures was found on the percentage of milk fat and fat : protein ratio (P-value < 0.05). The time of test-day milking and milk sampling had a significant impact on the level of all examined milk parameters (P-value < 0.001). Moreover, milk yield and fat-corrected milk yield were significantly affected by a feeding group × time of test-day milking and milk sampling interaction, influencing the level of examined parameters.</p><p><strong>Conclusion: </strong>The use of herbal supplements in the diet of dairy goats did not negatively affect the goats' milk production - neither the yield nor composition. A positive effect of the administered multi-herbal mixtures was found on % fat concentration and fat : protein ratio in milk.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 2","pages":"263-272"},"PeriodicalIF":1.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological and molecular screening for zoonotic pathogens among wild hedgehogs (<i>Erinaceus europaeus</i>) from urban areas of Poland.","authors":"Hanna Turlewicz-Podbielska, Jakub Jędrzej Ruszkowski, Maria Pisarek, Łukasz Adaszek, Małgorzata Pomorska-Mól","doi":"10.2478/jvetres-2025-0022","DOIUrl":"10.2478/jvetres-2025-0022","url":null,"abstract":"<p><strong>Introduction: </strong>Wild European hedgehogs (<i>Erinaceus europaeus</i>) can carry various pathogens potentially harmful to humans. This study was conducted to determine the occurrence of selected zoonotic pathogens in European hedgehogs from urban areas of central-western Poland.</p><p><strong>Material and methods: </strong>Sixty-nine samples (43 of sera and 26 spleens) were collected from 54 hedgehogs brought to the Wildlife Rehabilitation Centre in Poznań, Poland, between June 2020 and September 2023. Antibodies against <i>Coxiella burnetii</i>, hepatitis E virus genotype 3, <i>Toxoplasma gondii</i> and <i>Trichinella</i> spp. in serum samples were determined using commercial ELISA tests. A PCR was used to evaluate the prevalence of <i>Anaplasma</i> spp., <i>Ehrlichia</i> spp., <i>Borrelia</i> spp., <i>Rickettsia</i> spp. and <i>Leptospira</i> spp. genetic material in spleens.</p><p><strong>Results: </strong>The genetic material of <i>Anaplasma phagocytophilum</i> was found in 18 out of 26 spleens (69.23%; 95% confidence interval (CI): 50.01-83.50), and the genetic material of <i>Rickettsia helvetica</i> in 4 out of these 26 (15.38 %; 95% CI: 6.15-33.53). All <i>Rickettsia</i>-positive spleens were also positive for <i>Anaplasma</i> spp. None of the other pathogens or antibodies against them were detected.</p><p><strong>Conclusion: </strong>This study provides valuable insights into the prevalence of some zoonotic pathogens in urban hedgehog populations and their potential impact on public health and urban biodiversity.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 2","pages":"219-226"},"PeriodicalIF":1.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of virulence factors and antimicrobial susceptibility of <i>Citrobacter freundii</i> isolated from diseased ornamental fish in Poland.","authors":"Anna Pastuszka, Leszek Guz, Krzysztof Puk, Dorota Pietras-Ożga","doi":"10.2478/jvetres-2025-0017","DOIUrl":"10.2478/jvetres-2025-0017","url":null,"abstract":"<p><strong>Introduction: </strong><i>Citrobacter freundii</i> strains, like other representatives of the same genus, are often described as aetiological factors of diseases in humans and animals, including farmed and ornamental fish. The aim of the study was the isolation and identification of <i>C. freundii</i> from ornamental fish in Poland and investigation of the virulence factors and antibiotic resistance among the isolates.</p><p><strong>Material and methods: </strong>Twenty <i>C. freundii</i> isolates were isolated from swab samples obtained from diseased ornamental fish of 16 different species in Poland. The bacteria were initially identified to the species level by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and the results were confirmed based on sequencing of a fragment of the <i>16S rRNA</i> gene and biochemical profile analysis using the API-20E system. The antibiotic susceptibility profile was evaluated by the disc-diffusion method.</p><p><strong>Results: </strong>Most isolates were resistant to tetracycline (65%), ciprofloxacin (65%), nalidixic acid (60%) and doxycycline (50%). All of them were resistant to ampicillin. As many as 13/20 isolates (65%) carried the <i>viaB</i> gene encoding the Vi antigen and 9/20 (45%) carried the class 1 integron-encoding gene. The <i>hlyA</i> gene encoding the ability to produce α-haemolysins was not detected in any of the isolates tested.</p><p><strong>Conclusion: </strong>This is the first study to describe in detail the identification and occurrence of virulence factors and antibiotic resistance among <i>C. freundii</i> isolates of ornamental fish in Poland. The results indicate the need for further monitoring of the bacterium's presence.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"17-26"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of probiotics on immune cells in young Japanese Black calves responding to vaccination against bacterial respiratory diseases.","authors":"Shogo Takeda, Hiromichi Ohtsuka, Keigo Kosenda","doi":"10.2478/jvetres-2025-0013","DOIUrl":"10.2478/jvetres-2025-0013","url":null,"abstract":"<p><strong>Introduction: </strong>The vaccination against bacterial respiratory diseases in calves has been generally recognised as useful for the prevention of infections. Inducing an immunological response after vaccination is important for obtaining protection from infections. The aim of the study was to investigate the effects of probiotics on the immunological response to vaccination against bacterial respiratory diseases in young Japanese Black calves.</p><p><strong>Material and methods: </strong>Twenty-four Japanese Black calves were randomly divided into two groups (12 calves for the research group and 12 calves for the control group) on the seventh day of life (dol). The research group received 30 g per day of live bacteria mix consisting of <i>Streptococcus faecalis, Clostridium butyricum</i> and <i>Bacillus mesentericus</i> until the 63^rd dol. The control group did not receive the bacteria mix. All calves were vaccinated against bacterial respiratory diseases twice, at 21 and 42 dol. Blood samples were obtained from all calves at 7, 21, 42 (prior to the second vaccination), 45, 49 and 63 dol for determination of antibody titres, leukocyte numbers and cytokine genes.</p><p><strong>Results: </strong>Lymphocyte counts, T cell (CD3⁺, CD4⁺ and CD8⁺ cell) counts and relative expressions of cytokine genes (interleukin (IL)-4 and IL-17A) at 45, 49 and 63 dol were significantly higher in the research group compared than in the control group.</p><p><strong>Conclusion: </strong>The addition of probiotics to young Japanese Black calves' feed promoted an immunological reaction to vaccination against bacterial respiratory diseases.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"27-33"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The tissue distribution of nucleobindin-2/nesfatin-1 in the reproductive organs of bitches with regard to the animal's age and body weight.","authors":"Marta Rybska, Marek Skrzypski, Karolina Pusiak, Tatiana Wojciechowicz, Adam Mieldzioc","doi":"10.2478/jvetres-2025-0015","DOIUrl":"10.2478/jvetres-2025-0015","url":null,"abstract":"<p><strong>Introduction: </strong>Neuropeptide nesfatin-1, a nucleobindin-2 derivative, plays a role in regulating food intake, energy metabolism and body weight. It also interacts with the hypothalamic-pituitary-gonadal axis and has functions in the reproductive system. However, its impact on the canine reproductive tract has not been well documented. This study aimed to investigate the potential role of nesfatin-1 in canine ovarian activity and uterine function.</p><p><strong>Material and methods: </strong>Tissue and peripheral blood samples were collected from 60 bitches of various ages and body condition scores (BCS). Analyses included real-time PCR, immunofluorescence examinations and ELISA tests.</p><p><strong>Results: </strong>Higher level of nucleobindin-2 mRNA were found in the ovarian tissue of both younger and elderly overweight dogs (BCS > 5/9). The elevated expression of nesfatin-1 was observed in the uterine tissues of overweight dogs (BCS > 5/9) compared to its expression in animals in optimal body condition (BCS = 4/9). This finding was consistent with higher nesfatin-1 levels in the peripheral blood of overweight dogs.</p><p><strong>Conclusion: </strong>The distribution and expression of nesfatin-1 in canine reproductive organs vary depending on the animal's age and body weight. The role of nesfatin-1 in the reproductive system is influenced by the animal's body condition and the extent of surplus adipose tissue, which may have significant implications for reproductive functions.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"141-150"},"PeriodicalIF":1.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}