Prevalence of Giardia duodenalis genetic assemblages isolated from dogs and cats in Poland.

IF 1.5 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Veterinary Research Pub Date : 2025-08-28 eCollection Date: 2025-09-01 DOI:10.2478/jvetres-2025-0043

Dawid Jańczak, Jakub Olszewski, Aleksandra Kornelia Maj, Piotr Górecki, Magdalena Nowak, Olga Szaluś-Jordanow

{"title":"Prevalence of Giardia duodenalis genetic assemblages isolated from dogs and cats in Poland.","authors":"Dawid Jańczak, Jakub Olszewski, Aleksandra Kornelia Maj, Piotr Górecki, Magdalena Nowak, Olga Szaluś-Jordanow","doi":"10.2478/jvetres-2025-0043","DOIUrl":null,"url":null,"abstract":"Introduction: Giardia duodenalis (syn. G. intestinalis and G. lamblia) is a protozoan intestinal parasite that infects various vertebrates. Genetic analyses of G. duodenalis have identified eight genetic assemblages (groups), designated A-H. This study aimed to determine the prevalence of G. duodenalis in household dogs and cats in Poland, analyse isolates by assemblage and relate assemblage results to zoonotic potential.Material and methods: From January to February 2024, 1,937 faecal samples from dogs and 1,077 samples from cats were examined microscopically for G. duodenalis cysts using the zinc flotation method. Positive samples were retained for further molecular tests. Genetic analysis was performed on the basis of the β-giardin locus which is 511 base pairs long. The obtained sequences were compared with reference sequences in the GenBank database.Results: Giardia duodenalis cysts were detected in 11.3% (219/1,937) of dogs and 7.06% (76/1,077) of cats using the zinc flotation method. Assemblages B, C, D and F were identified in 11, 77, 126 and 5 dogs, respectively. All isolates from cats were identified as assemblage F.Conclusion: The high rate of infection in pets increases the risk of transmission of zoonotic Giardia genetic assemblages to humans.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 3","pages":"447-455"},"PeriodicalIF":1.5000,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12503213/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2478/jvetres-2025-0043","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/9/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Giardia duodenalis (syn. G. intestinalis and G. lamblia) is a protozoan intestinal parasite that infects various vertebrates. Genetic analyses of G. duodenalis have identified eight genetic assemblages (groups), designated A-H. This study aimed to determine the prevalence of G. duodenalis in household dogs and cats in Poland, analyse isolates by assemblage and relate assemblage results to zoonotic potential.

Material and methods: From January to February 2024, 1,937 faecal samples from dogs and 1,077 samples from cats were examined microscopically for G. duodenalis cysts using the zinc flotation method. Positive samples were retained for further molecular tests. Genetic analysis was performed on the basis of the β-giardin locus which is 511 base pairs long. The obtained sequences were compared with reference sequences in the GenBank database.

Results: Giardia duodenalis cysts were detected in 11.3% (219/1,937) of dogs and 7.06% (76/1,077) of cats using the zinc flotation method. Assemblages B, C, D and F were identified in 11, 77, 126 and 5 dogs, respectively. All isolates from cats were identified as assemblage F.

Conclusion: The high rate of infection in pets increases the risk of transmission of zoonotic Giardia genetic assemblages to humans.

Abstract Image

查看原文本刊更多论文

波兰从狗和猫中分离出的十二指肠贾第虫基因组合的流行程度。

十二指肠贾第鞭毛虫（Giardia duodenalis）是一种感染多种脊椎动物的肠道原生寄生虫。通过遗传分析，鉴定出8个遗传组合（群），命名为A-H。本研究旨在确定波兰家养狗和猫中十二指肠弧菌的流行情况，通过组合分析分离株，并将组合结果与人畜共患的可能性联系起来。材料与方法：于2024年1 - 2月，采用锌浮选法对1937份犬粪便标本和1077份猫粪便标本进行十二指肠棘球蚴囊显微镜检查。阳性样本被保留作进一步的分子检测。对长511个碱基对的β-栀子苷位点进行遗传分析。将获得的序列与GenBank数据库中的参考序列进行比较。结果：锌浮选法检出十二指肠贾第虫包囊率分别为11.3%（219/ 1937）和7.06%（76/ 1077）。分别在11只、77只、126只和5只狗中鉴定出组合B、C、D和F。结论：宠物的高感染率增加了人畜共患贾第鞭毛虫基因组合传播给人类的风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Veterinary Research Veterinary-General Veterinary

CiteScore

0.90

自引率

5.60%

发文量

审稿时长

18 weeks

期刊介绍： Journal of Veterinary Research (formerly Bulletin of the Veterinary Institute in Pulawy) is a quarterly that publishes original papers, review articles and short communications on bacteriology, virology, parasitology, immunology, molecular biology, pathology, toxicology, pharmacology, and biochemistry. The main emphasis is, however, on infectious diseases of animals, food safety and public health, and clinical sciences.