Journal of water and health最新文献_第3页

Epidemiological surveillance of astrovirus, norovirus, rotavirus, and enterovirus in sewage (2022-2023) in Giza, Egypt. 2022-2023年埃及吉萨市污水中星状病毒、诺如病毒、轮状病毒和肠病毒的流行病学监测

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-05-01 Epub Date: 2025-05-06 DOI: 10.2166/wh.2025.324

Mohamed Nasr Fathi Shaheen, Nehal Ismail Ahmed, Elmahdy Mohamed Elmahdy

{"title":"Epidemiological surveillance of astrovirus, norovirus, rotavirus, and enterovirus in sewage (2022-2023) in Giza, Egypt.","authors":"Mohamed Nasr Fathi Shaheen, Nehal Ismail Ahmed, Elmahdy Mohamed Elmahdy","doi":"10.2166/wh.2025.324","DOIUrl":"https://doi.org/10.2166/wh.2025.324","url":null,"abstract":"The majority of people with enterically transmitted viruses excrete significant amounts of the virus in their feces for several days or weeks. Therefore, viruses causing diarrhea could be detected in the feces of infected persons and wastewater. In this study, the presence of human astrovirus (AstV), norovirus (NoV), rotavirus (RV), and enterovirus (EntV) was analyzed by real-time RT-PCR in raw sewage (n = 96), treated sewage (n = 96) and diarrheal stool samples (n = 200). Overall, 92.7% (89/96) of raw sewage samples and 48% (46/96) of treated sewage tested positive for at least one virus. The highest detection rates of the four viruses in raw sewage were observed in the winter season. Overall, the mean concentration of the four viruses was 7.3 log10 in raw and 4.8 log10 in treated wastewater, for a total removal of 34% of viral loads. In clinical samples, the most commonly detected virus was EntV followed by RV, NoV, and AstV. The mean concentrations of the four viruses in clinical samples ranged between 2.5 × 101 and 9.86 × 107 GC/g. The results presented here demonstrated that the environmental surveillance of entric viruses in sewage is a useful tool for the study of their transmission dynamics in humans and their molecular epidemiology.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 5","pages":"587-601"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of faecal indicator organisms to manage microbial health risks in recreational waterways not impacted by point sources of sewage: a systematic review of the epidemiological evidence. 使用粪便指示生物来管理未受点源污水影响的娱乐水道中的微生物健康风险：对流行病学证据的系统审查。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-05-01 Epub Date: 2025-05-08 DOI: 10.2166/wh.2025.304

Sonya Kozak, Anne Roiko, Katharina Gutjahr-Holland, Warish Ahmed, Cameron Veal, Paul Fisher, Simon Toze, Mark Weir, Helen Stratton

{"title":"The use of faecal indicator organisms to manage microbial health risks in recreational waterways not impacted by point sources of sewage: a systematic review of the epidemiological evidence.","authors":"Sonya Kozak, Anne Roiko, Katharina Gutjahr-Holland, Warish Ahmed, Cameron Veal, Paul Fisher, Simon Toze, Mark Weir, Helen Stratton","doi":"10.2166/wh.2025.304","DOIUrl":"https://doi.org/10.2166/wh.2025.304","url":null,"abstract":"This PRISMA review investigated the extent to which epidemiological evidence supports the use of faecal indicator organisms (FIOs) to manage microbial health risks in recreational waters without point sources of sewage. The quality of papers meeting the inclusion criteria was appraised using the Office of Health Assessment and Translation (OHAT) Risk of Bias tool and low-bias studies were synthesised. Studies consistently reported elevated illness risks (particularly gastrointestinal) among bathers compared with non-bathers. However, no FIOs or pathogens were associated consistently with any health outcomes. While enterococci most frequently correlated with a variety of illnesses, the relatively even split of positive and negative associations suggests an overall lack of association. Consequently, applying FIO guidelines derived from epidemiological studies with point sources of sewage could result in type I and type II errors. Overall, results suggest that the sources and drivers of health risks are site-specific. Tools including sanitary surveys, microbial source tracking, epidemiology and quantitative microbial risk assessment provide avenues for characterising site-specific health risks, for those who can afford them. Meanwhile, characterising the site-specific sources/drivers of contamination seems pragmatic as the limited evidence so far suggests that FIO monitoring may not be sufficient to protect health in these waters.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 5","pages":"563-586"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the timing and regional distribution of 18 psychoactive drugs and metabolites in a Chinese city by wastewater analysis. 利用废水分析评价某城市18种精神药物及其代谢物的时间和区域分布。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-05-01 Epub Date: 2025-04-15 DOI: 10.2166/wh.2025.325

Shuai Yuan, Yue Xiao, Ruxin Luo, Xin Wang, Ping Xiang

{"title":"Evaluation of the timing and regional distribution of 18 psychoactive drugs and metabolites in a Chinese city by wastewater analysis.","authors":"Shuai Yuan, Yue Xiao, Ruxin Luo, Xin Wang, Ping Xiang","doi":"10.2166/wh.2025.325","DOIUrl":"https://doi.org/10.2166/wh.2025.325","url":null,"abstract":"In recent years, mental disorders have become serious health problems. Monitoring the usage of psychotropic drugs can reflect the prevalence of mental disorders in the population, and wastewater analysis is an effective method for monitoring the use of psychoactive pharmaceuticals. In our study, we collected 58 samples from 12 wastewater treatment plants (WWTPs) in a southern city in China. We then analyzed the concentrations and loads of 18 frequently used psychoactive drugs and corresponding metabolites. The most abundant compounds in wastewater were diazepam, sulpiride, clozapine, venlafaxine, and quetiapine, with a detection frequency close to 100%. Sulpiride had the highest average load, followed by venlafaxine, clozapine, quetiapine, and diazepam. The higher load of diazepam in suburban WWTPs than in urban WWTPs reflects the large numbers of older people living in suburban areas in most of China. The lower loads of most target compounds in suburban WWTPs than in urban WWTPs indicate a greater likelihood of mental disorders in urban people than in suburban people. A correlation analysis of the target compounds revealed a correlation between the occurrence of venlafaxine, sulpiride, and clozapine. Overall, wastewater analysis is expected to be a significant tool for monitoring the consumption of psychoactive pharmaceuticals.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 5","pages":"602-614"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lead-contaminated groundwater exposes residents to health risks in Makululu, Zambia. 在赞比亚的马库卢卢，铅污染的地下水使居民面临健康风险。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-05-01 Epub Date: 2025-04-01 DOI: 10.2166/wh.2025.343

Tasha Siame, Kaampwe Muzandu, Keagan K Mulenga, Catherine B Dzombe

{"title":"Lead-contaminated groundwater exposes residents to health risks in Makululu, Zambia.","authors":"Tasha Siame, Kaampwe Muzandu, Keagan K Mulenga, Catherine B Dzombe","doi":"10.2166/wh.2025.343","DOIUrl":"https://doi.org/10.2166/wh.2025.343","url":null,"abstract":"Groundwater contamination by lead (Pb) occurs commonly in historically mined regions and presents health risks to exposed residents. Poor documentation of elevated Pb levels in underserved regions prevents the development of environmental contamination policies and interventions. This study investigates Pb contamination and associated carcinogenic and non-carcinogenic health risks in Makululu, a large compound near the former Pb mine in Kabwe City, Zambia. We analyzed 34 drinking groundwater samples from hand pump boreholes (n = 21) and shallow wells (n = 13). Pb concentrations exceeded WHO and USEPA standards for safe drinking in 100% of boreholes (median = 0.15 mg/L) and 77% of shallow wells (median = 0.06 mg/L). Linear regression analysis identified temperature and depth as key predictors of Pb concentrations, with higher temperatures correlating with increased Pb levels (p = 0.008), and deeper boreholes (50-100 m) exhibiting significantly higher Pb concentrations (p = 0.007) than shallow wells (10 m). Health risk assessments revealed that adults consuming borehole water had an average daily dose of 0.005 mg/kg/day of Pb exceeding WHO thresholds, with hazard quotients (HQ > 1) indicating non-carcinogenic risks. The findings highlight the urgent need for policies to mitigate environmental pollution, enforce water quality standards to reduce Pb levels and protect public health in Makululu.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 5","pages":"615-629"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative implications of tobacco and non-tobacco crop farming on aquatic ecosystems: a multi-index evaluation of irrigation suitability and pollution risks. 烟草和非烟草作物种植对水生生态系统的比较影响：灌溉适宜性和污染风险的多指标评价。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-05-01 Epub Date: 2025-05-09 DOI: 10.2166/wh.2025.022

Anupam Roy, M G Mostafa

{"title":"Comparative implications of tobacco and non-tobacco crop farming on aquatic ecosystems: a multi-index evaluation of irrigation suitability and pollution risks.","authors":"Anupam Roy, M G Mostafa","doi":"10.2166/wh.2025.022","DOIUrl":"https://doi.org/10.2166/wh.2025.022","url":null,"abstract":"Agriculture plays a dual role, both contributing to and being impacted by water pollution. This study evaluates the impact of tobacco and non-tobacco farming on irrigation suitability, water quality index (WQI), water health index (WHI), heavy metal (HM) pollution, and ecological risk (ER). Twenty-four water samples from six tobacco fields (TFs) and six non-tobacco fields (NTFs), collected before cropping season and after cropping season, were analyzed for major physico-chemical properties and HMs. The results suggest that TF improves sodium and alkalinity hazards but worsens nutrient and HM pollution (Pb: 0.119 mg/L, Cd: 0.021 mg/L, Ni: 0.242 mg/L), and threatens salinity, permeability, osmotic potential (OPπ), dry residue (RS), and miscellaneous hazards. Conversely, NTF has minimal impact, except for nutrient and miscellaneous hazards. WQI shifts from good to very poor, primarily driven by turbidity, PO4-P, K+, and NO3-N. Similarly, WHI declines from excellent to very poor in TF and from good to poor in NTF. HM pollution index has risen slightly above irrigation safety limits, mainly due to Cd, Ni, and Pb, resulting in a slightly elevated ER. This study demonstrates that TF, driven by excessive agrochemical use, renders water unsuitable for irrigation, underscoring the imperative for sustainable practices.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 5","pages":"648-670"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriological assessment of water quality in Barishal, Bangladesh: potability testing and antibiogram profiling. 孟加拉国巴里沙尔水质的细菌学评估：可饮用性测试和抗生素谱分析。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-04-01 Epub Date: 2025-03-11 DOI: 10.2166/wh.2025.258

Israt Jahan Mousumi, Abu Sayed, Mohammad Enamul Hoque Kayesh, Farzana Islam Rume

{"title":"Bacteriological assessment of water quality in Barishal, Bangladesh: potability testing and antibiogram profiling.","authors":"Israt Jahan Mousumi, Abu Sayed, Mohammad Enamul Hoque Kayesh, Farzana Islam Rume","doi":"10.2166/wh.2025.258","DOIUrl":"https://doi.org/10.2166/wh.2025.258","url":null,"abstract":"Water hygiene is a critical public health issue, particularly in developing countries like Bangladesh. Therefore, this study aimed to evaluate the bacteriological quality and antimicrobial resistance (AMR) patterns of bacterial isolates in drinking water sources commonly used by the public across Barishal district of Bangladesh. A total of 30 water samples were collected from six sources - household reserved tanks, ponds, rivers, hotels, farms, and street food vendor vans - between July 2022 and June 2023. Bacterial contamination was assessed using total viable count, most probable number (MPN), and selective culture methods, while antibiotic susceptibility testing (AST) was conducted to evaluate antimicrobial resistance (AMR) patterns. Results indicated substantial contamination, with the highest bacterial load observed in household reserved tank water (5.6 × 105 CFU/mL). Predominant bacterial isolates included Escherichia coli and Staphylococcus spp., suggesting fecal contamination and potential health risks. AMR profiling revealed resistance to beta-lactam antibiotics such as amoxicillin and penicillin, with multidrug resistance observed in E. coli, Staphylococcus spp., and non-lactose fermenting bacteria. However, susceptibility to colistin and levofloxacin was noted in some isolates. These findings underscore the urgent need for routine water quality monitoring, effective disinfection strategies, and public awareness initiatives to mitigate health risks and curb the spread of AMR in environmental water sources.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"450-460"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changing climate and socio-economic conditions as part of quantitative microbial risk assessment of surface drinking water sources: a review. 气候变化和社会经济条件作为地表饮用水源微生物风险定量评估的一部分：综述。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-04-01 Epub Date: 2025-03-20 DOI: 10.2166/wh.2025.486

M M Majedul Islam, Mia Bondelind, Viktor Bergion, Ekaterina Sokolova

{"title":"Changing climate and socio-economic conditions as part of quantitative microbial risk assessment of surface drinking water sources: a review.","authors":"M M Majedul Islam, Mia Bondelind, Viktor Bergion, Ekaterina Sokolova","doi":"10.2166/wh.2025.486","DOIUrl":"https://doi.org/10.2166/wh.2025.486","url":null,"abstract":"Climate and socio-economic changes are expected to significantly impact waterborne pathogens and associated health risks, yet the full extent of these effects remains unclear. Accurate quantification of these risks is crucial for informing effective interventions and policy decisions. Quantitative microbial risk assessment (QMRA) serves as a valuable tool for estimating the risk of infection caused by microorganisms in drinking water. This study reviews existing QMRA studies and tools in the context of surface water and drinking water provision. Most studies have implemented various steps of the QMRA framework but often without the application of specific QMRA tools. Although several QMRA tools address climatic factors, there are currently no tools that integrate socio-economic factors into their risk assessments. This study proposes an approach for incorporating both climatic and socio-economic factors into QMRA tools. Specifically, we suggest enhancements to the Swedish QMRA tool - an open-source tool that currently does not incorporate climate and socio-economic changes. Our proposed advancements aim to systematically account for future climatic and socio-economic impacts on health risks, providing a more comprehensive microbial risk assessment tool. These recommendations are also applicable to other QMRA tools, offering a pathway for their development and improving the overall assessment of microbial health risks.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"507-528"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum: Journal of Water and Health, 22 (11): 2218-2232: Sewer system sampling for wastewater-based disease surveillance: Is the work worth it? Urda Düker, Regina Nogueira, Estefania Carpio-Vallejo, Ingeborg Joost, Katharina Hüppe, Roland Suchenwirth, Yvonne Saathoff and Markus Wallner, https://dx.doi.org/10.2166/wh.2024.301. 更正：《水与卫生杂志》，22(11):2218-2232：污水系统采样用于基于废水的疾病监测：这项工作值得吗？Urda d<e:1> ker, Regina Nogueira, Estefania Carpio-Vallejo, Ingeborg Joost, Katharina h<e:1>, Roland Suchenwirth， Yvonne Saathoff和Markus Wallner， https://dx.doi.org/10.2166/wh.2024.301。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-04-01 DOI: 10.2166/wh.2025.002

引用次数: 0

Characterization of enteric pathogens in Harare, Zimbabwe using environmental surveillance and metagenomics. 利用环境监测和宏基因组学分析津巴布韦哈拉雷的肠道病原体特征。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-04-01 Epub Date: 2025-02-21 DOI: 10.2166/wh.2025.333

Samantha Cheung, Nicolette A Zhou, Vurayai Ruhanya, Kelsey J Jesser, Ignitious Nezomba, John Musvibe, Brendon Manyisa, George Nyandoro, Paradzai Chibukira, Arnold Mukaratirwa, Simon Takawira Muserere, Kudzai Masunda, Angelo Ong, John Scott Meschke

{"title":"Characterization of enteric pathogens in Harare, Zimbabwe using environmental surveillance and metagenomics.","authors":"Samantha Cheung, Nicolette A Zhou, Vurayai Ruhanya, Kelsey J Jesser, Ignitious Nezomba, John Musvibe, Brendon Manyisa, George Nyandoro, Paradzai Chibukira, Arnold Mukaratirwa, Simon Takawira Muserere, Kudzai Masunda, Angelo Ong, John Scott Meschke","doi":"10.2166/wh.2025.333","DOIUrl":"https://doi.org/10.2166/wh.2025.333","url":null,"abstract":"High diarrheal disease burden remains an urgent concern in low- and middle-income countries, greatly affecting children under the age of 5 years and those living with HIV and AIDS. Treatment of infectious diseases has also become increasingly difficult with the rapid rise of antimicrobial resistance (AMR). Environmental surveillance of wastewater can supplement gaps in clinical surveillance as residents on a sewage system contribute to the wastewater, providing simple, composite samples that can improve understanding about both pathogens and AMR in the community. This study evaluated the effectiveness of environmental surveillance with shotgun metagenomics as a tool to characterize a broad range of enteric pathogens, antibiotic resistance genes, and virulence factor genes (VFGs) in wastewater from six neighborhoods in Harare, Zimbabwe. Alpha and beta diversity of the microbial community were similar between high-income and low-income suburbs. Enteric pathogens of high AMR and clinical concern, including Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica, were detected in all samples. The top VFGs were encoded for delivery, adherence, and motility, functions important in toxin secretion, colonization, and immune modulation. The findings provide a foundation for future studies to explore environmental surveillance and shotgun metagenomics as a public health monitoring tool for enteric diseases.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"477-492"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing RT-qPCR multiplex assays for simultaneous detection of enteric and respiratory viruses in wastewater. 优化同时检测废水中肠道和呼吸道病毒的RT-qPCR多重检测方法。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-04-01 Epub Date: 2025-04-15 DOI: 10.2166/wh.2025.101

Tomás de Melo, Ashley Gedge, Denina B D Simmons, Jean-Paul Desaulniers, Andrea E Kirkwood

{"title":"Optimizing RT-qPCR multiplex assays for simultaneous detection of enteric and respiratory viruses in wastewater.","authors":"Tomás de Melo, Ashley Gedge, Denina B D Simmons, Jean-Paul Desaulniers, Andrea E Kirkwood","doi":"10.2166/wh.2025.101","DOIUrl":"https://doi.org/10.2166/wh.2025.101","url":null,"abstract":"This study presents the successful optimization of enteric RT-qPCR multiplex assays for detecting Norovirus GII, Enterovirus, and Coxsackievirus A6 or Enterovirus D68 in municipal wastewater samples. Additionally, optimization of a respiratory RT-qPCR multiplex assay to detect influenza A, respiratory syncytial virus, and SARS-CoV-2 was attempted. The enteric multiplex assays successfully detected Coxsackievirus A6 in wastewater during community outbreaks of hand-foot-mouth disease. Enterovirus D68 was also successfully detected in wastewater samples (Summer/Fall, 2022), which coincided with provincial public health reports of Enterovirus D68 cases. Attempting to optimize the respiratory multiplex assay resulted in challenges due to oligonucleotide cross-reactivity and cross-talk. Specifically, when Texas Red and FAM probes detected higher abundance targets, they interfered with the Cy5 and HEX fluorophore probes that detected lower-abundance targets. In contrast, selecting probes with Cy5/HEX for high-abundance targets and Texas Red/FAM for lower-abundance targets provided more robust results.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"546-560"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0