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Sewer system sampling for wastewater-based disease surveillance: Is the work worth it?
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 Epub Date: 2024-10-23 DOI: 10.2166/wh.2024.301
Urda Düker, Regina Nogueira, Estefania Carpio-Vallejo, Ingeborg Joost, Katharina Hüppe, Roland Suchenwirth, Yvonne Saathoff, Markus Wallner
{"title":"Sewer system sampling for wastewater-based disease surveillance: Is the work worth it?","authors":"Urda Düker, Regina Nogueira, Estefania Carpio-Vallejo, Ingeborg Joost, Katharina Hüppe, Roland Suchenwirth, Yvonne Saathoff, Markus Wallner","doi":"10.2166/wh.2024.301","DOIUrl":"https://doi.org/10.2166/wh.2024.301","url":null,"abstract":"<p><p>Wastewater treatment plant (WWTP) influent sampling is commonly used in wastewater-based disease surveillance to assess the circulation of pathogens in the population aggregated in a catchment area. However, the signal can be lost within the sewer network due to adsorption, degradation, and dilution processes. The present work aimed to investigate the dynamics of SARS-CoV-2 concentration in three sub-catchments of the sewer system in the city of Hildesheim, Germany, characterised by different levels of urbanisation and presence/absence of industry, and to evaluate the benefit of sub-catchment sampling compared to WWTP influent sampling. Our study shows that sampling and analysis of virus concentrations in sub-catchments with particular settlement structures allows the identification of high concentrations of the virus at a local level in the wastewater, which are lower in samples collected at the inlet of the treatment plant covering the whole catchment. Higher virus concentrations per inhabitant were found in the sub-catchments in comparison to the inlet of the WWTP. Additionally, sewer sampling provides spatially resolved concentrations of SARS-CoV-2 in the catchment area, which is important for detecting local high incidences of COVID-19.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2218-2232"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unveiling the silent information of wastewater-based epidemiology of SARS-CoV-2 at community and sanitary zone levels: experience in Córdoba City, Argentina.
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 Epub Date: 2024-10-18 DOI: 10.2166/wh.2024.285
Gisela Masachessi, Gonzalo Manuel Castro, María de Los Angeles Marinzalda, Ariana Mariela Cachi, Paola Sicilia, Veronica Emilce Prez, Laura Cecilia Martínez, Miguel Oscar Giordano, María Belen Pisano, Viviana Elizabeth Ré, Carlos Martin Del Bianco, Sofia Parisato, Micaela Fernandez, Gustavo Ibarra, Laura Lopez, Gabriela Barbás, Silvia Viviana Nates
{"title":"Unveiling the silent information of wastewater-based epidemiology of SARS-CoV-2 at community and sanitary zone levels: experience in Córdoba City, Argentina.","authors":"Gisela Masachessi, Gonzalo Manuel Castro, María de Los Angeles Marinzalda, Ariana Mariela Cachi, Paola Sicilia, Veronica Emilce Prez, Laura Cecilia Martínez, Miguel Oscar Giordano, María Belen Pisano, Viviana Elizabeth Ré, Carlos Martin Del Bianco, Sofia Parisato, Micaela Fernandez, Gustavo Ibarra, Laura Lopez, Gabriela Barbás, Silvia Viviana Nates","doi":"10.2166/wh.2024.285","DOIUrl":"https://doi.org/10.2166/wh.2024.285","url":null,"abstract":"<p><p>The emergence of COVID-19 in 2020 significantly enhanced the application of wastewater monitoring for detecting SARS-CoV-2 circulation within communities. From October 2021 to October 2022, we collected 406 wastewater samples weekly from the Córdoba Central Pipeline Network (BG-WWTP) and six specific sewer manholes from sanitary zones (SZs). Following WHO guidelines, we processed samples and detected SARS-CoV-2 RNA and variants using real-time PCR. Monitoring at the SZ level allowed for the development of a viral activity flow map, pinpointing key areas of SARS-CoV-2 circulation and tracking its temporal spread and variant evolution. Our findings demonstrate that wastewater-based surveillance acts as a sensitive indicator of viral activity, detecting imminent increases in COVID-19 cases before they become evident in clinical data. This study highlights the effectiveness of targeted wastewater monitoring at both municipal and SZ levels in identifying viral hotspots and assessing community-wide circulation. Importantly, the data shows that environmental wastewater studies provide valuable insights into virus presence, independent of clinical COVID-19 case records, and offer a robust tool for adapting to future public health challenges.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2171-2183"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The silent threat: Unveiling climate change's water and health challenges in Bangladesh.
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 Epub Date: 2024-10-18 DOI: 10.2166/wh.2024.349
Monira Parvin Moon
{"title":"The silent threat: Unveiling climate change's water and health challenges in Bangladesh.","authors":"Monira Parvin Moon","doi":"10.2166/wh.2024.349","DOIUrl":"10.2166/wh.2024.349","url":null,"abstract":"<p><p>Bangladesh faces a growing number of issues, such as limited sources of potable water and health hazards that are either directly or indirectly linked to climate change. In total, 16 publications from 2007 to 2024 were manually screened for inclusion in this systematic review. The articles were divided into three categories: climate and water, climate and health, and climate, water, and health. Due to the climate change, 20 million individuals in Bangladesh have been suffering from water- and health-related illness. The results showed that among Bangladeshi vulnerable groups, cholera, infant diarrhea, pneumonia, dengue, malaria, mental illness, hypertension, cardiovascular disease, cerebrovascular disease, and an increase in communicable diseases like water-, vector-, and food-borne infections, along with malnutrition, are common. Further findings of the study include health risks, water-related risks, and difficulties in determining the consequences of climate change. This review study focuses on Bangladesh, a developing country, and the correlation between climate change and the dangers of water and health-related diseases. The findings of this study have substantial implications for risk assessment, water quality, climate change, and public health, especially in poor nations, as well as for policymakers and academicians in these fields.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2094-2112"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of the physiochemical parameters on the occurrence of E. coli bacteria in a small and shallow reservoir.
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 Epub Date: 2024-10-22 DOI: 10.2166/wh.2024.394
Goran Volf, Ivana Sušanj Čule, Sonja Zorko
{"title":"Influence of the physiochemical parameters on the occurrence of <i>E. coli</i> bacteria in a small and shallow reservoir.","authors":"Goran Volf, Ivana Sušanj Čule, Sonja Zorko","doi":"10.2166/wh.2024.394","DOIUrl":"https://doi.org/10.2166/wh.2024.394","url":null,"abstract":"<p><p>The microbiological quality of water plays a crucial role in the relationship among human, animal, and environmental health. This research gives insight into the relationship between concentrations of <i>Escherichia coli</i> bacteria and physiochemical parameters in water, which is captured from the Butoniga reservoir and then used for treatment in the drinking water treatment plant Butoniga. Analysis was carried out using statistical analysis through the Pearson correlation coefficient and supported with PCA. The conducted analysis revealed that turbidity and Fe have the highest correlation coefficients with <i>E. coli</i> bacteria. Turbidity was also identified as a potential indicator for <i>E. coli</i> bacteria. Additionally, parameters such as Mn and UV 254 were also found to be closely related to <i>E. coli</i> bacteria, alongside turbidity and Fe. Furthermore, a relationship between <i>E. coli</i> bacteria and different water intakes was conducted. This shows that higher concentrations of <i>E. coli</i> bacteria were present when water was captured from lower water intakes, characterized by increased water turbidity. Thus, the research results provide important information on influential water quality parameters related to <i>E. coli</i> bacteria, especially in the Butoniga reservoir and related drinking water treatment plant, creating a foundation for future water quality management.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2206-2217"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cancer incidence associations with drinking water arsenic levels and disinfection methods in Maine, USA.
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 Epub Date: 2024-10-28 DOI: 10.2166/wh.2024.313
Jean D MacRae, Michael D Abbott, Gudeta D Fufaa
{"title":"Cancer incidence associations with drinking water arsenic levels and disinfection methods in Maine, USA.","authors":"Jean D MacRae, Michael D Abbott, Gudeta D Fufaa","doi":"10.2166/wh.2024.313","DOIUrl":"https://doi.org/10.2166/wh.2024.313","url":null,"abstract":"<p><p>Maine is a largely rural state where nearly half of the population uses drinking water from private wells. Arsenic (As) is present in some Maine groundwater, has been linked to cancer, and a lack of testing and treatment may expose people with private wells to elevated As levels. Disinfection byproducts (DBPs) include known and suspected carcinogens that form when chlorine or chloramines are added to water. People served by public water systems may be exposed to elevated levels of regulated DBPs such as trihalomethanes and haloacetic acids associated with chlorine and/or unregulated nitrogenous DBPs, or N-DBPs, such as nitrite and N-nitrosodimethylamine associated with chloramines. Contrary to initial expectations, there were no significant associations between median town As in well water and bladder, lung, kidney, or skin cancer incidence. Furthermore, bladder, melanoma, and other skin cancer incidence rates were negatively correlated with the percent of the town population using private wells. Analysis of cancer incidence associated with chlorine and chloramine disinfection showed elevated melanoma, and other skin cancer with chloramine use and elevated bladder and non-melanoma skin cancer with chlorine use compared to the no disinfectant case. We recommend more research on the links between disinfectant use and cancer.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2246-2256"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficiency evaluation of wastewater treatment by three macrophytes using a pilot-constructed wetland system in Ota, Nigeria.
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 Epub Date: 2024-10-18 DOI: 10.2166/wh.2024.116
Lazarus D Justin, David O Olukanni
{"title":"Efficiency evaluation of wastewater treatment by three macrophytes using a pilot-constructed wetland system in Ota, Nigeria.","authors":"Lazarus D Justin, David O Olukanni","doi":"10.2166/wh.2024.116","DOIUrl":"https://doi.org/10.2166/wh.2024.116","url":null,"abstract":"<p><p>Three aquatic macrophytes were used to treat wastewater using a pilot-constructed wetland (CW) system to determine the most efficient plants for removing contaminants from wastewater. The three macrophytes are water hyacinth (<i>Eichhornia crassipes</i>), water lettuce (<i>Pistia stratiotes</i>), and duckweed (<i>Lemna minor</i>). Three 150 L capacity tanks with sand and gravel as substrates were used as the pilot CW for each plant. Upon initial examination, the raw wastewater was not compliant with standard discharge limits. The wastewater samples were collected every 7 days for 3 weeks for treatment. From the findings, at 14 days hydraulic retention time (HRT), <i>E. crassipes</i> and <i>P. stratiotes</i> achieved the highest total phosphorus (TP) and chemical oxygen deman (COD) reductions of 99.3 and 99.4%, respectively. <i>E. crassipes</i> indicated better biological oxygen demand removal efficiency of 91.3%, COD (85.0%), electrical conductivity (90.4%), total dissolved solids (89.7%), and total coliforms (66.0%). Albeit, <i>P. stratiotes</i> indicated better results for total suspended solids (96.2%), TP (7.55%), and <i>E. coli</i> (94.4%), while <i>L. minor</i> was better with 90.8% total nitrogen removal. The overall analysis showed <i>E. crassipes</i> to be more efficient than the three macrophytes. However, the other two plants are replaceable options and large-scale implementation of this project in the community would be a major contributor to actualizing SDG number 6.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2040-2053"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pathogen validation of small- and large-scale recycled water plants utilizing various clarification and media filtration technologies.
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.2166/wh.2024.263
Petra Reeve, Gretchen Marshall, Po Zhang, Ben Thwaites, Ben van den Akker
{"title":"Pathogen validation of small- and large-scale recycled water plants utilizing various clarification and media filtration technologies.","authors":"Petra Reeve, Gretchen Marshall, Po Zhang, Ben Thwaites, Ben van den Akker","doi":"10.2166/wh.2024.263","DOIUrl":"https://doi.org/10.2166/wh.2024.263","url":null,"abstract":"<p><p>Media filters are important in wastewater recycling schemes for pathogen removal. Filter selection depends on health targets and plant scale; however, there is a data gap concerning pathogen removal efficacy at full scale. This study compared the pathogen removal performance of two full-scale filtration technologies, including a small 17,000 m<sup>3</sup>/d pressurized media filtration (PMF) plant and a large 120,000 m<sup>3</sup>/d gravity filter in the form of dissolved air flotation filtration (DAFF). The preceding clarification processes were also assessed. Validation of protozoa and virus removal was estimated by dosing model organisms yeast and MS2 bacteriophage to demonstrate removal potential. The DAFF process (coagulation, flotation and filtration) was most efficient at removing bacteriophage with a mean log<sub>10</sub> reduction value (LRV) of 2.90 (±0.64), compared with 0.98 (±0.37) achieved by coagulation, sedimentation and PMF. Yeast log<sub>10</sub> reduction though both systems were similar measuring 3.80 (±1.06) through DAFF and 4.57 (±0.14) through coagulation, sedimentation and PMF. The DAFF process showed greater variability in MS2 and yeast removal, which was attributed to filtration. Energy and chemical usage were also evaluated, revealing trade-offs between these factors, treatment scale and pathogen LRVs, offering practical insights into the technological and economic aspects of designing fit-for-purpose recycled water schemes.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2132-2145"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the experiences of the overburden of water collection responsibility of rural women in Ghana.
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 Epub Date: 2024-10-25 DOI: 10.2166/wh.2024.033
Gervin Ane Apatinga, Corinne Schuster-Wallace, Sarah Dickson-Anderson
{"title":"Exploring the experiences of the overburden of water collection responsibility of rural women in Ghana.","authors":"Gervin Ane Apatinga, Corinne Schuster-Wallace, Sarah Dickson-Anderson","doi":"10.2166/wh.2024.033","DOIUrl":"https://doi.org/10.2166/wh.2024.033","url":null,"abstract":"<p><p>Despite evidence emphasizing women's responsibility for collecting water in sub-Saharan Africa, more needs to be known about the gender-specific consequences of this obligation, especially in rural Ghana, where water inaccessibility is a persistent issue. Employing a community-based case study, this research aimed to explore the gendered consequences of women's water collection responsibility, using a coupled systems framework. Data were gathered from surveys and focus groups and analysed statistically and thematically, respectively. Key findings highlighted intersecting influences in women's water access and collection difficulties, including distance to water sources, poverty, and health issues. Results revealed that over 50% of women experienced multiple consequences, including physical and psychological injuries (>80%), animal attacks (≤12%), spousal violence (>40%), nutritional challenges (>30%), hygiene problems (>40%), and socioeconomic issues (>50%). Over half faced three to seven intersecting water-related consequences, which intensified their difficulty in accessing and collecting water. Differences were observed across sub-communities. Interestingly, not all men had knowledge of these consequences, highlighting the crucial need to broaden their understanding as part of the solution to ease women's burdens. Addressing sociocultural norms and the various factors influencing access through effective and gendered water management and planning is imperative to alleviate women's burdens and improve equitable access.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2015-2039"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Legionella spp. in influent wastewater in Kobe City, Japan.
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 Epub Date: 2024-10-18 DOI: 10.2166/wh.2024.167
Shoko Komatsu, Chinami Fujinaga, Noriko Nakanishi
{"title":"Detection of <i>Legionella</i> spp. in influent wastewater in Kobe City, Japan.","authors":"Shoko Komatsu, Chinami Fujinaga, Noriko Nakanishi","doi":"10.2166/wh.2024.167","DOIUrl":"https://doi.org/10.2166/wh.2024.167","url":null,"abstract":"<p><p><i>Legionella</i> is an important waterborne pathogen that causes Legionnaires' disease (LD). Several outbreaks associated with wastewater treatment plants (WWTPs) have been reported in recent years; however, the prevalence of <i>Legionella</i> in WWTPs in Japan has rarely been investigated. In this study, we investigated the distribution of <i>Legionella</i> in influent wastewater collected from two WWTPs in Kobe, Japan between April 2023 and March 2024. The concentrations for <i>Legionella</i> in all seasons varied between 10<sup>4</sup> and 10<sup>6</sup> copies/100 mL in all seasons. Among the 10 <i>Legionella</i> species detected in the influent wastewater, <i>Legionella pneumophila</i> was the most commonly isolated. Genotyping revealed that pathogenic <i>L. pneumophila</i> strains were widely distributed in the influent wastewater in Japan with genetic diversity. LD is one of the most important infectious diseases during natural disasters. This study highlights the importance of influent wastewater as a potential source of LD in Japan, where natural disasters occur frequently.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2054-2059"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Safe drinking water: to what extent are shallow wells reliable?
IF 2.5 4区 环境科学与生态学
Journal of water and health Pub Date : 2024-11-01 DOI: 10.2166/wh.2024.388
Bruna Suellen Breternitz, Milena Dropa, Solange Martone-Rocha, Pedro Smith Pereira Ferraro, Francisca Alzira Dos Santos Peternella, Miriam Lopes da Silva, Maria Tereza Pepe Razzolini
{"title":"Safe drinking water: to what extent are shallow wells reliable?","authors":"Bruna Suellen Breternitz, Milena Dropa, Solange Martone-Rocha, Pedro Smith Pereira Ferraro, Francisca Alzira Dos Santos Peternella, Miriam Lopes da Silva, Maria Tereza Pepe Razzolini","doi":"10.2166/wh.2024.388","DOIUrl":"https://doi.org/10.2166/wh.2024.388","url":null,"abstract":"<p><p>Our study investigated the presence of <i>Cryptosporidium hominis-parvum-meleagridis</i> and <i>Giardia duodenalis</i> in shallow wells used for drinking water (DW) in a rural area. Also, bovine feces were collected from their vicinity. Our findings highlight significant potential risks associated with these pathogens in DW sources. Due to the COVID-19 pandemic, samples were collected in two phases: September/2019 to March/2020 and October/2021 to April/2022. Water samples (<i>n</i> = 69) and feces (<i>n</i> = 13) were collected biweekly and analyzed using the USEPA 1623.1 method and molecular tools for species identification, and feces by direct DNA extraction. During the first phase, oocysts and cysts were detected at frequencies ranging from 9.1 to 41.7 and 27.3 to 83.3, respectively. In the second phase, oocyst frequencies ranged from not detected to 25%, while cyst ranged from 18.2 to 83.3%. <i>Escherichia coli</i> concentrations were significant at all collection points. <i>C. hominis-parvum-meleagridis</i> and <i>G. duodenalis</i> were notably detected in bovine feces. Our study revealed the presence of anthropogenic parasites and <i>E. coli</i> in DW sources intended for human consumption. These findings underscore the urgent need for preventive measures to ensure safe DW and prevent future contamination. Effective water quality surveillance is crucial in this regard<b>.</b></p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 11","pages":"2184-2193"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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