Journal of water and health最新文献

Comparative evaluation of heating instruments for detecting Bacteroides HF183, SARS-CoV-2, and Aichi virus A in untreated wastewater using colorimetric LAMP. 比色LAMP法检测废水中拟杆菌HF183、SARS-CoV-2和爱知病毒A的加热仪器比较评价

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-05-05 DOI: 10.2166/wh.2025.327

Mariah C A do Nascimento, Wendy J M Smith, Yawen Liu, Vivaldo Gomes da Costa, Stuart L Simpson, Aaron Bivins, Paula Rahal, Warish Ahmed

{"title":"Comparative evaluation of heating instruments for detecting Bacteroides HF183, SARS-CoV-2, and Aichi virus A in untreated wastewater using colorimetric LAMP.","authors":"Mariah C A do Nascimento, Wendy J M Smith, Yawen Liu, Vivaldo Gomes da Costa, Stuart L Simpson, Aaron Bivins, Paula Rahal, Warish Ahmed","doi":"10.2166/wh.2025.327","DOIUrl":"https://doi.org/10.2166/wh.2025.327","url":null,"abstract":"This study assessed the accuracy, field suitability, and cost-effectiveness of colorimetric LAMP (c-LAMP) assays using six heating instruments: Electric Kettle, Water Bath, Heating Block, miniPCR, Thermocycler, and Genie® III. Bacteroides HF183 (HF183), SARS-CoV-2, and Aichi virus A (AiV-A) were tested using control materials and wastewater samples, comparing cLAMP with qPCR/RT-qPCR detection. qPCR/RT-qPCR reliably detected HF183 and AiV-A genetic fragments at dilutions as low as 1 × 10-5 pg/μL, corresponding to 1.24 and 1.49 log10 GC/reaction. cLAMP/RT-cLAMP detected these fragments at 1 × 10-4 pg/μL (2.28 and 2.63 log10 GC/reaction), though Electric Kettle, miniPCR, and Genie® III occasionally produced false negatives. RT-qPCR reliably detected SARS-CoV-2 fragments at 1 × 100 GC/μL (1.86 log10 GC/reaction), with cLAMP/RT-cLAMP performing similarly across most instruments except Thermocycler. No significant differences (p > 0.05) were observed among instruments, though inconsistencies appeared at lower concentrations. The findings offer valuable insights into heating instrument performance for cLAMP/RT-cLAMP assays, guiding their application in wastewater-based pathogen detection.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"701-714"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Particulate contaminants and treatment decision-making: maximizing the value of raw water pathogen monitoring for drinking water safety. 颗粒污染物与处理决策：原水病原体监测对饮用水安全的价值最大化。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-05-09 DOI: 10.2166/wh.2025.020

Dafne de Brito Cruz, Philip Schmidt, Kelsey Kundert, Norma Ruecker, Monica Emelko

{"title":"Particulate contaminants and treatment decision-making: maximizing the value of raw water pathogen monitoring for drinking water safety.","authors":"Dafne de Brito Cruz, Philip Schmidt, Kelsey Kundert, Norma Ruecker, Monica Emelko","doi":"10.2166/wh.2025.020","DOIUrl":"https://doi.org/10.2166/wh.2025.020","url":null,"abstract":"Periodic evaluation of particulate contaminants in raw/untreated water is integral to assessing risk, establishing treatment requirements, and ensuring drinking water safety. However, pathogenic microorganisms and other discrete particles (e.g., microplastics) are not typically monitored with any regularity. When monitoring is required, recommended, or proactively used to evaluate the adequacy of treatment or assess treatment needs, there is a need for guidance on how to collect data and use them to maximize return on investment. The potentially increasing variability in source water quality associated with climate change emphasizes the importance of knowing contaminant concentrations to effectively manage risks. This work presents a framework to guide the development of monitoring protocols for particulate contaminants in water and the integration of monitoring data and quantitative microbial risk assessment into treatment decisions. The protozoa monitoring and risk-based compliance approach of a drinking water utility in Canada is presented along with 7 years of data. Guidance for determining sampling frequencies and locations is provided. It is shown that Cryptosporidium monitoring may be insufficient to inform treatment needs when Giardia cysts are more abundant in source water. This work underscores the importance of revisiting and enhancing monitoring practices for effective treatment and public health protection.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"764-779"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and evaluation of recycled glass sand as water filtration media. 再生玻璃砂作为水过滤介质的特性与评价。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-06-09 DOI: 10.2166/wh.2025.023

Shehbaz Ahmad, Fernanda Mac-Allister Cedraz, Nicholas Borne, Julie N L Albert, Tiong Gim Aw

{"title":"Characterization and evaluation of recycled glass sand as water filtration media.","authors":"Shehbaz Ahmad, Fernanda Mac-Allister Cedraz, Nicholas Borne, Julie N L Albert, Tiong Gim Aw","doi":"10.2166/wh.2025.023","DOIUrl":"https://doi.org/10.2166/wh.2025.023","url":null,"abstract":"Recycled glass offers a promising, cost-effective alternative to silica sand for water filtration. This study evaluated its performance in a gravity-driven flow system using three particle sizes: gravel (G), coarse sand (CS), and fine sand (FS). As expected, a tradeoff was observed between turbidity reduction and permeability. FS achieved the greatest turbidity reduction (96.6% in particulate filtration and 93.1% in environmental water filtration) and Escherichia coli log removal of 1 ± 0.2, but low permeability. Higher permeability but poor turbidity and E. coli removal was achieved using G. To balance these tradeoffs, a layered filtration system was used to improve permeability with effective turbidity reduction (96.9% in particulate filtration and 93.5% in environmental water filtration). Without coagulant treatment, the E. coli log removal was 0.27 ± 0.15; with coagulant pre-treatment, it increased to 2.5 ± 0.4 for the layered filtration system. These findings demonstrate that crushed recycled glass can be used as an effective filtration medium and the filtration system can be configured with different particle sizes and/or layers to meet application-specific requirements.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"780-793"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy metals in drinking water sources in northern Mexico: a review of concentrations and human health risks assessment. 墨西哥北部饮用水源中的重金属：浓度审查和人类健康风险评估。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-05-13 DOI: 10.2166/wh.2025.274

Mayra Ramírez-Cota, Ofelia Escobar-Sánchez, Miguel Betancourt-Lozano, Martín Gabriel Frías-Espericueta, Nydia Yuriana Zamora-Arellano, Carmen Cristina Osuna-Martínez

{"title":"Heavy metals in drinking water sources in northern Mexico: a review of concentrations and human health risks assessment.","authors":"Mayra Ramírez-Cota, Ofelia Escobar-Sánchez, Miguel Betancourt-Lozano, Martín Gabriel Frías-Espericueta, Nydia Yuriana Zamora-Arellano, Carmen Cristina Osuna-Martínez","doi":"10.2166/wh.2025.274","DOIUrl":"https://doi.org/10.2166/wh.2025.274","url":null,"abstract":"Despite the high concentrations of heavy metals (HMs) in the water bodies of northern Mexico, current monitoring efforts and regulations are insufficient. This is especially troubling, given the indigenous and vulnerable communities and the overall lack of public health information. To characterize exposure, we conducted an exhaustive review of HM studies in drinking water in northern Mexico. This information was used to estimate hazard quotient (HQ) and carcinogenic risk (CR) values by age for all of northern Mexico. In total, 316 concentrations of HMs were reported in 55 studies from 1975 to 2021, with concentration ranges of 0-11,100, 0-5,250, 0-350, 0-25, and 0-9,470 μg/L for arsenic, cadmium, chromium, mercury, and lead, respectively. The probable maximum concentrations (95th percentile) of metals in drinking water were 244.55, 10, 56, 1.68, and 90.35 μg/L for arsenic, cadmium, chromium, mercury, and lead, respectively. The HQ for all HMs ranged from 0.0011 to 404.62. Children younger than 2 years had an extremely high risk (HQ > 40) of presenting adverse health effects from arsenic consumption. Children younger than 6 years had the highest risk of developing cancer, with CR values from 2.24 × 10-5 to 7.77 × 10-1, emphasizing the need for continuous HM monitoring in northern Mexico.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"684-700"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sharp decline of diarrhea hospitalizations despite historical drought in Brazil: The potential role of local water supply and public health measures. 尽管巴西历史干旱，但腹泻住院人数急剧下降：当地供水和公共卫生措施的潜在作用。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-05-21 DOI: 10.2166/wh.2025.392

Alexandre Cunha Costa, Tahissa Frota Cavalcante, Andressa Suelly Saturnino de Oliveira, Iran Eduardo Lima Neto, Germano Gondim Ribeiro Neto, Pieter Richard van Oel, Rafaella Pessoa Moreira

{"title":"Sharp decline of diarrhea hospitalizations despite historical drought in Brazil: The potential role of local water supply and public health measures.","authors":"Alexandre Cunha Costa, Tahissa Frota Cavalcante, Andressa Suelly Saturnino de Oliveira, Iran Eduardo Lima Neto, Germano Gondim Ribeiro Neto, Pieter Richard van Oel, Rafaella Pessoa Moreira","doi":"10.2166/wh.2025.392","DOIUrl":"https://doi.org/10.2166/wh.2025.392","url":null,"abstract":"Severe droughts (SDs) present a risk to public health, particularly in terms of the proliferation of waterborne diseases, such as diarrhea. Because of climate change, the frequency of SDs is likely to increase, potentially straining traditional water supply systems. An 11-year period of SDs in Northeast Brazil, which commenced in 2012, reduced the capacity of 153 drinking water reservoirs to a mere 6.7%, with 86% of water bodies exhibiting eutrophic characteristics. During this period, several water supply and public health measures were implemented. Those measures were associated with a reduction in the incidence of diarrhea hospitalizations during the entire SD period, with an average annual reduction of -4.9%. This assessment identifies potential adaptive measures to mitigate the impact of climate change on water-related human health, thus supporting the implementation of measures to protect against climate risks to public health.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"743-756"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of land use on water quality at different spatial scales in the area around Chaohu Lake region. 环巢湖地区不同空间尺度土地利用对水质的影响

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-06-05 DOI: 10.2166/wh.2025.057

Sheng Li, Chunlin Wang, Xi Zhang, Zhiyuan Gong, Rui Zhang

{"title":"The impact of land use on water quality at different spatial scales in the area around Chaohu Lake region.","authors":"Sheng Li, Chunlin Wang, Xi Zhang, Zhiyuan Gong, Rui Zhang","doi":"10.2166/wh.2025.057","DOIUrl":"https://doi.org/10.2166/wh.2025.057","url":null,"abstract":"This study focuses on the area around Chaohu Lake region, utilizing data from the 2021 national surface water quality monitoring stations and land use data from territorial surveys. Employing GIS spatial analysis, non-parametric tests, Spearman correlation analysis, and redundancy analysis (RDA), the research examines how land use types within different buffer zone scales affect water quality. The findings indicate: (1) The dominant land use types in the study area are cultivated land, construction land, and water areas. (2) Overall, water quality is better in the dry season than in the rainy season, with higher concentrations of CODMn and TP occurring in the rainy season, and higher concentrations of DO, NH3-N, and TN in the dry season. (3) Cultivated land and construction land are positively correlated with all water quality indices, whereas forest lands and water areas generally exhibit a negative correlation. The correlation between grasslands and water quality indices alternates with changes in spatial scale. (4) Within a buffer zone of 3,000 m, land use has the greatest impact on water quality, making it the optimal scale for assessing the influence of land use on water quality indices in the area around Chaohu Lake region (93.35%).","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"838-852"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skilled nursing facility wastewater surveillance: a SARS-CoV-2 and antimicrobial resistance detection pilot study. 熟练护理机构废水监测：SARS-CoV-2和抗菌素耐药性检测中试研究

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-05-09 DOI: 10.2166/wh.2025.374

Ariel Jose Santiago, Maria Burgos Garay, Mariya Campbell, Yimu Cahela, Rodney Donlan, Paige Gable, Christine Ganim Kyros, Lauren Franco, Leila Kartforosh, Susanna Lenz, Amanda K Lyons, Jamari Moore, Judith Noble-Wang, Carrie Sanders, Bethelhem Abera, Colin H Adler, Sophie Jones, Magdalena Medrzycki, Maroya S Walters, Peter Cook, Yan Li, Ying Tao, Jing Zhang, Lakshmi Malapati, Adam Retchless, Suxiang Tong, Angela D Coulliette-Salmond

{"title":"Skilled nursing facility wastewater surveillance: a SARS-CoV-2 and antimicrobial resistance detection pilot study.","authors":"Ariel Jose Santiago, Maria Burgos Garay, Mariya Campbell, Yimu Cahela, Rodney Donlan, Paige Gable, Christine Ganim Kyros, Lauren Franco, Leila Kartforosh, Susanna Lenz, Amanda K Lyons, Jamari Moore, Judith Noble-Wang, Carrie Sanders, Bethelhem Abera, Colin H Adler, Sophie Jones, Magdalena Medrzycki, Maroya S Walters, Peter Cook, Yan Li, Ying Tao, Jing Zhang, Lakshmi Malapati, Adam Retchless, Suxiang Tong, Angela D Coulliette-Salmond","doi":"10.2166/wh.2025.374","DOIUrl":"https://doi.org/10.2166/wh.2025.374","url":null,"abstract":"The purpose of this study was to determine the feasibility of facility-level wastewater surveillance in the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in skilled nursing facility (SNF) wastewater using three concentration methods, as well as a proof-of-concept for antimicrobial resistance (AR) genes/organisms detection. Wastewater effluent samples were collected from an SNF over an 8-week period. Wastewater was concentrated using electronegative membrane filtration (enMF), polyethylene glycol precipitation, and Nanotrap® magnetic virus particles (NP). Quantification of the genome copy concentration from SARS-CoV-2 and bovine respiratory syncytial virus (BRSV), a SARS-CoV-2 surrogate spiked into all samples, was performed with droplet digital polymerase chain reaction (ddPCR). Wastewater sample aliquots were also enriched in microbiological culture media and screened for organisms with AR phenotypes on selective and differential agars. Multiplex real-time PCR was used to detect a broad array of carbapenem resistance genes. SARS-CoV-2 was detected and quantified from a single enMF-concentrated wastewater sample. The highest concentration of BRSV came from enMF-concentrated samples. Klebsiella, Enterobacter, Citrobacter, and Escherichia coli exhibiting AR phenotypes were successfully detected using culture-dependent approaches. Culture-independent, multiplex PCR indicated that blaKPC was the main carbapenemase gene detected in wastewater samples. Facility-level wastewater surveillance could be a useful strategy for SNFs.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"727-742"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing well characteristics as risk factors for bacterial contamination of private wells in Wellington-Dufferin-Guelph, Ontario. 评估油井特征作为安大略省Wellington-Dufferin-Guelph私人水井细菌污染的危险因素。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-05-09 DOI: 10.2166/wh.2025.046

Leslie G Fell, Lorna E Deeth, Olaf Berke, Lise A Trotz-Williams

{"title":"Assessing well characteristics as risk factors for bacterial contamination of private wells in Wellington-Dufferin-Guelph, Ontario.","authors":"Leslie G Fell, Lorna E Deeth, Olaf Berke, Lise A Trotz-Williams","doi":"10.2166/wh.2025.046","DOIUrl":"https://doi.org/10.2166/wh.2025.046","url":null,"abstract":"This study aims to identify important well characteristics associated with increased odds of bacterial contamination in the Wellington-Dufferin-Guelph public health unit of Southern Ontario. Identifying risk factors associated with bacterial contamination can aid in the mandate of public health units to promote the safety, and facilitate the testing, of drinking water systems to help minimize the risk of illness. Logistic regression models for adverse bacterial test results based on physical well characteristics were created. Models with the lowest Akaike Information Criterion values were examined for consistently identified characteristics. The odds of bacterial contamination in the Wellington-Dufferin-Guelph region are most associated with the age of the well, the season of testing, having a treatment system on the well, and the presence of potential point contamination sources within 50 feet (15.24 m) of the well. While this information can support the design of targeted public health education campaigns, the current model leaves room for improvement, as the predictive abilities of the models based solely on well characteristic data are limited.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"826-837"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wastewater and environmental surveillance for Vibrio cholerae: a scoping review. 霍乱弧菌废水和环境监测：范围综述。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-05-20 DOI: 10.2166/wh.2025.351

Renée Street, Sizwe Nkambule, Nomfundo Mahlangeni, Mashudu Mthethwa, Ntombifuthi Blose, Bettina Genthe, Tamara Kredo

{"title":"Wastewater and environmental surveillance for Vibrio cholerae: a scoping review.","authors":"Renée Street, Sizwe Nkambule, Nomfundo Mahlangeni, Mashudu Mthethwa, Ntombifuthi Blose, Bettina Genthe, Tamara Kredo","doi":"10.2166/wh.2025.351","DOIUrl":"https://doi.org/10.2166/wh.2025.351","url":null,"abstract":"There is growing interest in the utility of wastewater and environmental surveillance (WES) in supporting disease outbreaks, including the monitoring of Vibrio cholerae. The objective of this scoping review was to describe the current evidence regarding the surveillance of V. cholerae in wastewater and wastewater-impacted environmental waters (including drinking water). We conducted a comprehensive electronic search, updated to January 2025. Following duplicate screening, we identified 114 eligible studies. The studies evaluated early WES warning systems, monitored trends in ongoing outbreaks, and described serogroups and virulent strains of toxigenic V. cholerae circulating in communities. Most studies were conducted in South-East Asia (44%), particularly India and Bangladesh, where cholera is endemic. A large proportion of studies detected V. cholerae subgroup O1 or O139, and numerous virulent strains such as ctxA/B. Studies were generally poorly reported, for example, inconsistent reporting on sample management, data reliability, and sampling frequency were common. WES has not been widely integrated into existing surveillance systems for real-time cholera monitoring. Our findings underscore the need for further clearly reported research to clarify the role of WES for early warning systems for cholera outbreaks, and to identify strategies that may optimise WES implementation for public health benefit.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"715-726"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved recovery of Cryptosporidium and Giardia in water by optimizing immunomagnetic separation using acid and heat dissociation. 优化免疫磁选法对水中隐孢子虫和贾第鞭毛虫的回收率。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2025-06-01 Epub Date: 2025-05-16 DOI: 10.2166/wh.2025.006

Eun-Sook Lee, Ji-Yeon Lee, Young-Ae Baek, Seok-Ju Cho

{"title":"Improved recovery of Cryptosporidium and Giardia in water by optimizing immunomagnetic separation using acid and heat dissociation.","authors":"Eun-Sook Lee, Ji-Yeon Lee, Young-Ae Baek, Seok-Ju Cho","doi":"10.2166/wh.2025.006","DOIUrl":"https://doi.org/10.2166/wh.2025.006","url":null,"abstract":"It is important to efficiently recover (oo)cysts in water during the established detection process, as Cryptosporidium and Giardia are known to cause waterborne outbreaks. We investigated the recovery of these (oo)cysts based on the pH of HCl and NaOH used during the immunomagnetic separation (IMS) step. Additionally, we evaluated the efficiency of heat dissociation as a potential alternative to acid dissociation in IMS. The results showed that recovery rates decreased as the final pH deviated from neutral. The highest recovery rates, exceeding 60%, were observed when the pH of 0.1 N HCl and 1 N NaOH fell within the range of 0.9-1.0 and 13.0-13.1, respectively. The pH had a greater impact on cysts than on oocysts. Since the absolute zeta potential of cysts is lower than that of oocysts, cysts may be less stable in water and more sensitive to pH variations. The recovery rates of (oo)cysts were comparable when either acid and heat dissociation was applied. Therefore, to enhance the recovery of (oo)cysts in environmental water, it is essential to regularly monitor the pH of the reagent used in IMS step. Furthermore, the application of heat dissociation without HCl and NaOH may be considered as an alternative method.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 6","pages":"757-763"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0