Israt Jahan Mousumi, Abu Sayed, Mohammad Enamul Hoque Kayesh, Farzana Islam Rume
{"title":"Bacteriological assessment of water quality in Barishal, Bangladesh: potability testing and antibiogram profiling.","authors":"Israt Jahan Mousumi, Abu Sayed, Mohammad Enamul Hoque Kayesh, Farzana Islam Rume","doi":"10.2166/wh.2025.258","DOIUrl":"https://doi.org/10.2166/wh.2025.258","url":null,"abstract":"<p><p>Water hygiene is a critical public health issue, particularly in developing countries like Bangladesh. Therefore, this study aimed to evaluate the bacteriological quality and antimicrobial resistance (AMR) patterns of bacterial isolates in drinking water sources commonly used by the public across Barishal district of Bangladesh. A total of 30 water samples were collected from six sources - household reserved tanks, ponds, rivers, hotels, farms, and street food vendor vans - between July 2022 and June 2023. Bacterial contamination was assessed using total viable count, most probable number (MPN), and selective culture methods, while antibiotic susceptibility testing (AST) was conducted to evaluate antimicrobial resistance (AMR) patterns. Results indicated substantial contamination, with the highest bacterial load observed in household reserved tank water (5.6 × 10<sup>5</sup> CFU/mL). Predominant bacterial isolates included <i>Escherichia coli</i> and <i>Staphylococcus</i> spp., suggesting fecal contamination and potential health risks. AMR profiling revealed resistance to beta-lactam antibiotics such as amoxicillin and penicillin, with multidrug resistance observed in <i>E. coli</i>, <i>Staphylococcus</i> spp., and non-lactose fermenting bacteria. However, susceptibility to colistin and levofloxacin was noted in some isolates. These findings underscore the urgent need for routine water quality monitoring, effective disinfection strategies, and public awareness initiatives to mitigate health risks and curb the spread of AMR in environmental water sources.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"450-460"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M M Majedul Islam, Mia Bondelind, Viktor Bergion, Ekaterina Sokolova
{"title":"Changing climate and socio-economic conditions as part of quantitative microbial risk assessment of surface drinking water sources: a review.","authors":"M M Majedul Islam, Mia Bondelind, Viktor Bergion, Ekaterina Sokolova","doi":"10.2166/wh.2025.486","DOIUrl":"https://doi.org/10.2166/wh.2025.486","url":null,"abstract":"<p><p>Climate and socio-economic changes are expected to significantly impact waterborne pathogens and associated health risks, yet the full extent of these effects remains unclear. Accurate quantification of these risks is crucial for informing effective interventions and policy decisions. Quantitative microbial risk assessment (QMRA) serves as a valuable tool for estimating the risk of infection caused by microorganisms in drinking water. This study reviews existing QMRA studies and tools in the context of surface water and drinking water provision. Most studies have implemented various steps of the QMRA framework but often without the application of specific QMRA tools. Although several QMRA tools address climatic factors, there are currently no tools that integrate socio-economic factors into their risk assessments. This study proposes an approach for incorporating both climatic and socio-economic factors into QMRA tools. Specifically, we suggest enhancements to the Swedish QMRA tool - an open-source tool that currently does not incorporate climate and socio-economic changes. Our proposed advancements aim to systematically account for future climatic and socio-economic impacts on health risks, providing a more comprehensive microbial risk assessment tool. These recommendations are also applicable to other QMRA tools, offering a pathway for their development and improving the overall assessment of microbial health risks.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"507-528"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Corrigendum: <i>Journal of Water and Health</i>, 22 (11): 2218-2232: Sewer system sampling for wastewater-based disease surveillance: Is the work worth it? Urda Düker, Regina Nogueira, Estefania Carpio-Vallejo, Ingeborg Joost, Katharina Hüppe, Roland Suchenwirth, Yvonne Saathoff and Markus Wallner, https://dx.doi.org/10.2166/wh.2024.301.","authors":"","doi":"10.2166/wh.2025.002","DOIUrl":"https://doi.org/10.2166/wh.2025.002","url":null,"abstract":"","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"561-562"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samantha Cheung, Nicolette A Zhou, Vurayai Ruhanya, Kelsey J Jesser, Ignitious Nezomba, John Musvibe, Brendon Manyisa, George Nyandoro, Paradzai Chibukira, Arnold Mukaratirwa, Simon Takawira Muserere, Kudzai Masunda, Angelo Ong, John Scott Meschke
{"title":"Characterization of enteric pathogens in Harare, Zimbabwe using environmental surveillance and metagenomics.","authors":"Samantha Cheung, Nicolette A Zhou, Vurayai Ruhanya, Kelsey J Jesser, Ignitious Nezomba, John Musvibe, Brendon Manyisa, George Nyandoro, Paradzai Chibukira, Arnold Mukaratirwa, Simon Takawira Muserere, Kudzai Masunda, Angelo Ong, John Scott Meschke","doi":"10.2166/wh.2025.333","DOIUrl":"https://doi.org/10.2166/wh.2025.333","url":null,"abstract":"<p><p>High diarrheal disease burden remains an urgent concern in low- and middle-income countries, greatly affecting children under the age of 5 years and those living with HIV and AIDS. Treatment of infectious diseases has also become increasingly difficult with the rapid rise of antimicrobial resistance (AMR). Environmental surveillance of wastewater can supplement gaps in clinical surveillance as residents on a sewage system contribute to the wastewater, providing simple, composite samples that can improve understanding about both pathogens and AMR in the community. This study evaluated the effectiveness of environmental surveillance with shotgun metagenomics as a tool to characterize a broad range of enteric pathogens, antibiotic resistance genes, and virulence factor genes (VFGs) in wastewater from six neighborhoods in Harare, Zimbabwe. Alpha and beta diversity of the microbial community were similar between high-income and low-income suburbs. Enteric pathogens of high AMR and clinical concern, including <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Salmonella enterica</i>, were detected in all samples. The top VFGs were encoded for delivery, adherence, and motility, functions important in toxin secretion, colonization, and immune modulation. The findings provide a foundation for future studies to explore environmental surveillance and shotgun metagenomics as a public health monitoring tool for enteric diseases.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"477-492"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tomás de Melo, Ashley Gedge, Denina B D Simmons, Jean-Paul Desaulniers, Andrea E Kirkwood
{"title":"Optimizing RT-qPCR multiplex assays for simultaneous detection of enteric and respiratory viruses in wastewater.","authors":"Tomás de Melo, Ashley Gedge, Denina B D Simmons, Jean-Paul Desaulniers, Andrea E Kirkwood","doi":"10.2166/wh.2025.101","DOIUrl":"https://doi.org/10.2166/wh.2025.101","url":null,"abstract":"<p><p>This study presents the successful optimization of enteric RT-qPCR multiplex assays for detecting Norovirus GII, Enterovirus, and Coxsackievirus A6 or Enterovirus D68 in municipal wastewater samples. Additionally, optimization of a respiratory RT-qPCR multiplex assay to detect influenza A, respiratory syncytial virus, and SARS-CoV-2 was attempted. The enteric multiplex assays successfully detected Coxsackievirus A6 in wastewater during community outbreaks of hand-foot-mouth disease. Enterovirus D68 was also successfully detected in wastewater samples (Summer/Fall, 2022), which coincided with provincial public health reports of Enterovirus D68 cases. Attempting to optimize the respiratory multiplex assay resulted in challenges due to oligonucleotide cross-reactivity and cross-talk. Specifically, when Texas Red and FAM probes detected higher abundance targets, they interfered with the Cy5 and HEX fluorophore probes that detected lower-abundance targets. In contrast, selecting probes with Cy5/HEX for high-abundance targets and Texas Red/FAM for lower-abundance targets provided more robust results.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"546-560"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative analysis of ARIMA and Holt's models for predicting water and sanitation access in Bangladesh: toward SDG target.","authors":"Sacchidanand Majumder, Soma Chowdhury Biswas","doi":"10.2166/wh.2025.388","DOIUrl":"https://doi.org/10.2166/wh.2025.388","url":null,"abstract":"<p><p>This study analyzes the trend and forecasts the future proportion of the people in Bangladesh using safely managed drinking water and sanitation services, aiming for progress toward SDGs. The annual datasets cover the years 2000-2022 and are sourced from the World Bank Databank, focusing on 'Proportion of people using safely managed drinking water services' and 'Proportion of people using safely managed sanitation services.' Initially, the Mann-Kendall test is applied to detect seasonal trends in the time series data. The ARIMA (0,1,0) model is identified as the optimal fit for forecasting drinking water services, while Holt's method is preferred for sanitation services. Results show an upward trend in both areas; however, the rates remain inadequate to meet SDG 6 targets. Projections indicate that by 2025, 60.6% of the population will have access to safely managed drinking water and 33.5% will have access to sanitation services, whereas the Bangladeshi government aims for 75 and 80%, respectively. Furthermore, by 2030, these proportions are expected to increase to 63.7% for drinking water and 37.2% for sanitation. This analysis suggests that, if current trends continue, the national targets and the SDG targets 6.1 and 6.2 will not be achievable by 2030.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"529-545"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ehsan Javanmard, Elham Kazemirad, Hanieh Mohammad Rahimi, Mehdi Mohebali, Mostafa Rezaeian, Mahmood Alimohammadi, Hamed Mirjalali
{"title":"Prevalence of free-living amoebae in five rivers associated with high human activity in Tehran province, Iran.","authors":"Ehsan Javanmard, Elham Kazemirad, Hanieh Mohammad Rahimi, Mehdi Mohebali, Mostafa Rezaeian, Mahmood Alimohammadi, Hamed Mirjalali","doi":"10.2166/wh.2025.350","DOIUrl":"https://doi.org/10.2166/wh.2025.350","url":null,"abstract":"<p><p>Free-living amoebae (FLA) are ubiquitous protozoa capable of enduring harsh environmental conditions. These microorganisms are commonly found in water, soil, and air and can be transmitted to humans in areas with high human activity. This study aimed to investigate the prevalence of FLA and their associated genotypes/species in five rivers in Tehran province, Iran. A total of 60 water samples were collected from the Jajrud, Kan, Farhzad, Darakeh, and Shadchay rivers. Samples were subjected to filtration and cultivation onto non-nutrient agar. The genera/species of FLA were characterized based on the amplification and sequencing of the specific genetic fragments. Microscopic analysis suggested the presence of trophozoites and cysts of FLA in 18/60 (30%) of samples, of which <i>Acanthamoeba</i> spp., <i>Vermamoeba</i> sp., and Vahlkampfiidae were identified. Sequence analysis showed the presence of the genotypes T11, T4, T3, and T5 in five, five, four, and one isolates, respectively. The molecular analysis of the T4 genotype showed gene flow between the current isolates with previously described sequences. The findings suggest a clear association between environmental and clinical isolates of <i>Acanthamoeba</i> spp. Therefore, scheduled monitoring of environmental waters, particularly in regions with high human activities, is highly recommended.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"493-506"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterizing the co-existence of metallo-β-lactamase-producing and extended-spectrum β-lactamase-producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> isolates in community wastewater samples of Dhaka, Bangladesh.","authors":"Maftuha Ahmad Zahra, Tasfia Tasnim Toma, Shamima Nasreen, Zarin Tasnim Rafia Zarin, Zerin Tasnim Siddiqa Elma Khan, Fahim Kabir Monjurul Haque","doi":"10.2166/wh.2025.286","DOIUrl":"https://doi.org/10.2166/wh.2025.286","url":null,"abstract":"<p><p><i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> isolates with multiple antibiotic-resistance genes in wastewater pose serious public health risks, as they can potentially contaminate the food and water supply. The main aim of this study was to isolate and identify <i>E. coli</i> and <i>K. pneumoniae</i> from community wastewater samples, and determine their antibiotic-resistance profiles and their antibiotic-resistant genes. From the northern part of Dhaka, Bangladesh, 36 wastewater samples were collected across 11 different areas, which were then serially diluted, and cultured using selective media. Isolates were identified via polymerase chain reaction. Out of the 197 isolates identified, <i>E. coli</i> and <i>K. pneumoniae</i> accounted for 55.8% (<i>n</i> = 110) and 44.2% (<i>n</i> = 87), respectively. Antibiotic susceptibility tests revealed multidrug resistance (MDR) in 30% of <i>E. coli</i> and 35.56% of <i>K. pneumoniae</i> isolates. Among <i>E. coli</i>, the prevalence of antibiotic-resistance genes included <i>bla</i><sub>NDM-1</sub> (8.9%), <i>bla</i><sub>SHV</sub> (13.9%), and <i>bla</i><sub>CTX-M</sub> (7.6%). In <i>K. pneumoniae</i>, the percentages were <i>bla</i><sub>NDM-1</sub> (12.8%), <i>bla</i><sub>SHV</sub> (4.3%), and <i>bla</i><sub>CTX-M</sub> (5.0%). Co-existence of multiple antibiotic-resistance genes was observed in 4.54% of <i>E. coli</i> isolates (<i>n</i> = 5) and 5.74% of <i>K. pneumoniae</i> isolates (<i>n</i> = 5). This suggests the escalating issue of infectious species becoming increasingly resistant to antibiotics in wastewater systems.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"461-476"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comprehensive One Health investigation of erythromycin and quinupristin/dalfopristin resistant <i>Enterococcus</i> spp. in Iran.","authors":"Fereshteh Hasanpour, Farzad Khademi, Behnam Mohammadi Ghalehbin, Malek Namaki Kheljan, Elham Jannati, Sohrab Iranpour, Mohsen Arzanlou","doi":"10.2166/wh.2025.174","DOIUrl":"https://doi.org/10.2166/wh.2025.174","url":null,"abstract":"<p><p>Enterococci, flagged by the WHO as a rising cause of antibiotic-resistant infections, make surveillance crucial to control resistant strains. We investigated the resistance to linezolid, quinupristin/dalfopristin (Q/D), and erythromycin in <i>Enterococcus faecalis</i> (<i>n</i> = 251) and <i>Enterococcus faecium</i> (<i>n</i> = 434) isolates collected from patients, healthy carriers, hospitals, poultry, livestock, and municipal wastewater in Ardabil, Iran. The isolates were tested for resistance using phenotypic and genotypic methods. Although none of the isolates were resistant to linezolid, 24.9% of <i>E. faecium</i> isolates were resistant to Q/D, particularly those from patients and poultry slaughterhouse wastewater effluent (<i>P</i> < 0.05). The Q/D resistance genes <i>msrC</i> and <i>ermB</i> were detected in 76.85 and 20.37% of <i>E. faecium</i> isolates, respectively. Erythromycin resistance was common in <i>E. faecalis</i> (51.8%) and <i>E. faecium</i> (37.5%), with no significant difference between sources. However, isolates from patients and livestock wastewater had higher erythromycin MICs. Erythromycin resistance genes, such as <i>ermB</i>, <i>ermC</i>, <i>ermTR</i>, and <i>ermA</i>, were found in 80.7, 41.2, 26.5, and 19% of <i>E. faecium</i> and 80.3, 51.6, 22.4, and 25.8% of <i>E. faecalis</i> isolates, respectively. In conclusion, linezolid is a viable treatment for enterococcal infections in Ardabil, but widespread erythromycin- and Q/D-resistant enterococci pose a public health risk.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 4","pages":"439-449"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of drinking water quality test kits for home use in the United States.","authors":"LeighAnn D'Andrea, Emily Kumpel","doi":"10.2166/wh.2025.289","DOIUrl":"https://doi.org/10.2166/wh.2025.289","url":null,"abstract":"<p><p>Water consumers in the United States may want to test their drinking water using at-home commercially available test kits rather than a certified laboratory due to convenience and affordability. However, while numerous do-it-yourself test kits are available for purchase online or at local stores, these kits are unregulated and lack data on their performance. We evaluated off-the-shelf home drinking water test kits that measure iron, copper, manganese, and fluoride concentrations to investigate whether these kits could reliably provide meaningful results. We evaluated their performance in three water matrices: deionized water (DI), tap water, and river water, and with laboratory-trained personnel compared to untrained users. Our results showed highly repeatable but variable performance in the test kits' ability to detect potential contaminants in the water. Most kits performed best in the DI water matrix with no interference. Our results suggest that there are concerns about their accuracy and usefulness and that whether the results can be relied on depends on which parameter is being measured in which water with which kit and for which purpose.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"23 3","pages":"350-363"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}