Journal of water and health最新文献_第4页

Tracking SARS-CoV-2 variants in wastewater in San Pedro de la Paz, Chile. 追踪智利圣佩德罗德拉巴斯废水中的SARS-CoV-2变体。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-11-28 DOI: 10.2166/wh.2024.284

Andressa S Reis, Christian Castro, Paulina Assmann, Katherine Salgado, M Estrella Armijo, María José Navarrete, Cesar Echeverria, Aldo Gaggero, Carlos Farkas, Matias I Hepp

{"title":"Tracking SARS-CoV-2 variants in wastewater in San Pedro de la Paz, Chile.","authors":"Andressa S Reis, Christian Castro, Paulina Assmann, Katherine Salgado, M Estrella Armijo, María José Navarrete, Cesar Echeverria, Aldo Gaggero, Carlos Farkas, Matias I Hepp","doi":"10.2166/wh.2024.284","DOIUrl":"https://doi.org/10.2166/wh.2024.284","url":null,"abstract":"Studies have shown the presence of SARS-CoV-2 in the stool of both symptomatic and asymptomatic COVID-19 patients, enabling wastewater-based surveillance (WBS) to complement clinical monitoring. The emergence of variants can enhance viral transmissibility, highlighting the need for ongoing surveillance to detect and control infectious diseases. This study aimed to detect SARS-CoV-2 variants in wastewater from a treatment plant in San Pedro de la Paz, Chile, between January and November 2021. Wastewater samples were concentrated using the polyethylene glycol method, and RT-qPCR assays were performed to analyze SARS-CoV-2 and its variants (Alpha, Beta, Gamma, Lambda, and Delta), with results compared to Illumina amplicon sequencing. The concentration method achieved about 11% viral recovery. The detection of viruses and variants in wastewater proved sensitive and consistent with clinical data, providing additional surveillance insights. Notably, Lambda and Delta variants were the most frequently detected during the second and third infection waves, with some variants identified in wastewater before the first confirmed clinical cases. However, Illumina sequencing lacked sufficient genome coverage, suggesting the need for better sequencing methods for this matrix. This study demonstrates that WBS is a rapid, cost-effective tool for detecting SARS-CoV-2 and its mutations, particularly useful during overwhelming clinical situations or when cost is prohibitively high.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2398-2413"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluoride and nitrogen contamination and potential health risks in the groundwater of a typical agricultural region. 典型农业区地下水中氟氮污染及其潜在健康风险

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-11-19 DOI: 10.2166/wh.2024.234

Junzhu Yang, Jiangxia Wang, Han Xu, Zibo Xu, Yun Zhang, Jie Chen

{"title":"Fluoride and nitrogen contamination and potential health risks in the groundwater of a typical agricultural region.","authors":"Junzhu Yang, Jiangxia Wang, Han Xu, Zibo Xu, Yun Zhang, Jie Chen","doi":"10.2166/wh.2024.234","DOIUrl":"https://doi.org/10.2166/wh.2024.234","url":null,"abstract":"Fluoride and nitrogen contamination is a global concern and has been a serious problem in agricultural areas. This study aims to identify the source of fluoride and nitrogen in the groundwater and assess groundwater quality and human health risks in the Guanzhong Plain, northwest China. The results showed that the concentrations were 0.15-4.74 mg/L for F-, 0.02-89.89 mg/L for NO3--N, and BDL-2.40 mg/L for NH4+-N in groundwater. Distinct area-dependent distributions of fluoride and nitrogen were observed in the study region. Higher F- and NO3--N concentrations in groundwater were detected in the northern part, and higher NH4+-N levels were observed in the southern part. Water-rock interaction and agricultural activities were the controlling factors for fluoride and nitrogen distribution in groundwater. About 80% of samples are considered to have good water quality with WQI < 100. Exposure to fluoride and nitrogen through drinking should require more attention. The total non-carcinogenic risks through oral ingestion of groundwater were 0.22-3.19 for adults and 0.51-7.44 for children, respectively. The order of pollutants in the groundwater in terms of their hazard to residents was F- > NH4+-N > NO3--N > NO2--N. The findings of this study could provide more insights into groundwater management.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2331-2345"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer. 在HaCaT细胞单层上评估马来西亚巴生谷3个休闲湖泊病原环境棘阿米巴T4和T9基因型的体外动态。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-12-04 DOI: 10.2166/wh.2024.162

Rohaya Abdul Halim, Hasseri Halim, Rosnani Hanim Mohd Hussain, Shafiq Aazmi, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar

{"title":"Assessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer.","authors":"Rohaya Abdul Halim, Hasseri Halim, Rosnani Hanim Mohd Hussain, Shafiq Aazmi, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar","doi":"10.2166/wh.2024.162","DOIUrl":"https://doi.org/10.2166/wh.2024.162","url":null,"abstract":"Free-living amoebae of the genus Acanthamoeba are causative agents of keratitis and amoebic encephalitis. They are widely found in various ecological environments. Therefore, the present study brings results that can help to better understand the genotypes of the environmental isolates and their pathogenicity. This study procured 26 Acanthamoeba isolates from three recreational lakes in 2022. Polymerase chain reaction amplification was performed on positive Acanthamoeba samples. The thermotolerance, osmotolerance, and cytopathogenicity in human keratinocyte (HaCaT) cells of the samples were also evaluated. The phylogenetic analysis demonstrated that 12 isolates were of genotype T4, two (T9), six (T17), four (T8), and one each from T5 and T11. The thermo- and osmotolerance assays indicated that eight Acanthamoeba samples were potentially pathogenic. Two T4 and one T9 genotype also recorded 33-, 42-, and 133-kDa serine-type proteases, respectively. The HaCaT cell monolayer revealed that three T4 and one T9 samples achieved cytopathic effects within the 50-100% range, hence significantly cytotoxic. The lactate dehydrogenase secretion results demonstrated that three (T4) and one (T9) sample exhibited exceptional toxicity (over 40%) compared to the other samples. The responses of Acanthamoeba members with similar genotypes to pathogenicity indicator assays varied considerably, rendering correlation of pathogenicity with specific genotypes challenging.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2289-2303"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zambia's battle against cholera outbreaks and the path to public health resilience: a narrative review. 赞比亚抗击霍乱疫情和实现公共卫生复原力之路：叙述性审查。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-11-11 DOI: 10.2166/wh.2024.094

Bashar Haruna Gulumbe, Kennedy Chishimba, Aminu Shehu, Mary Chibwe

{"title":"Zambia's battle against cholera outbreaks and the path to public health resilience: a narrative review.","authors":"Bashar Haruna Gulumbe, Kennedy Chishimba, Aminu Shehu, Mary Chibwe","doi":"10.2166/wh.2024.094","DOIUrl":"https://doi.org/10.2166/wh.2024.094","url":null,"abstract":"In Zambia, cholera has been a persistent public health concern for decades, mainly attributed to inadequate sanitation and restricted access to clean water in some parts of the country. The literature was collected from PubMed, Google Scholar, and public health organization websites, focusing on cholera outbreaks in Zambia since 2000. Key search terms included 'cholera prevention' and 'Zambia outbreaks.' A total of 30 articles were analyzed to assess public health strategies and identify gaps for future planning. Periodic outbreaks of cholera have characterized Zambia's history by significant fluctuations in case numbers and fatalities since 1977. Notably, the years 1993, 1999, 2003/2004, 2005/2006, 2010, 2017/2018, and the most recent outbreak from October 2023 to February 2024 have marked significant episodes in the country's struggle against this waterborne disease. This narrative review examines the recurrent outbreaks of cholera in Zambia to understand the recent outbreak's extraordinary spread and severity in the context of public health resilience. The most recent outbreak with a staggering 19,719 cases and 682 deaths highlights the unprecedented scale and severity of the current public health emergency. The findings highlight the need for a holistic public health approach that prioritizes resilience in disease prevention.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2257-2275"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vertical stratification of the water microbiome in an electric water heater tank: implications for premise plumbing opportunistic pathogens. 电热水器水箱中水微生物群的垂直分层：对前提管道条件致病菌的影响。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-11-21 DOI: 10.2166/wh.2024.265

Vicente Gomez-Alvarez, Hodon Ryu, Morgan McNeely, Christy Muhlen, Daniel Williams, Darren Lytle, Laura Boczek

{"title":"Vertical stratification of the water microbiome in an electric water heater tank: implications for premise plumbing opportunistic pathogens.","authors":"Vicente Gomez-Alvarez, Hodon Ryu, Morgan McNeely, Christy Muhlen, Daniel Williams, Darren Lytle, Laura Boczek","doi":"10.2166/wh.2024.265","DOIUrl":"10.2166/wh.2024.265","url":null,"abstract":"Hot water systems are the most frequent environment associated with the prevalence and growth of opportunistic premise plumbing pathogens (OPPPs). Previous studies identified water heaters as a source of waterborne diseases and concluded that design variables may contribute to their prevalence. A multifaceted approach was used to investigate the vertical stratification of the microbiome and selected OPPPs in an electric water heater tank connected to a home plumbing system simulator. Results show that the microbiome is highly diverse with evidence of temperature stratification and temporal structuring influenced by the partial drainage of the tank. Representatives of the Mycobacterium spp. were the most prevalent taxa, followed by Legionella spp., and a relatively low prevalence of free-living amoeba Vermamoeba vermiformis. Higher concentrations of Legionella pneumophila at the bottom of the tank indicated the potential growth and protection of this opportunistic pathogen at this location. Overall, partial drainage of the water tank (60% of the tank capacity) did not significantly mitigate the microbiome and selected OPPPs. The outcome of this study sheds light on the role of vertical stratification on water quality and demonstrates the resilience of the microbial community residing in an electric water heater tank and the implications for public health.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2346-2357"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and antibiotic resistance patterns of Pseudomonas aeruginosa across different point sources of pollution in the Buriganga River, Bangladesh. 铜绿假单胞菌在孟加拉国布里甘加河不同污染源中的分布和抗生素耐药性模式。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-11-29 DOI: 10.2166/wh.2024.270

Dilara I Sharif, Forsan Amin, Md Hasib Mehbub, Rakibul Islam Ratul

{"title":"Distribution and antibiotic resistance patterns of Pseudomonas aeruginosa across different point sources of pollution in the Buriganga River, Bangladesh.","authors":"Dilara I Sharif, Forsan Amin, Md Hasib Mehbub, Rakibul Islam Ratul","doi":"10.2166/wh.2024.270","DOIUrl":"https://doi.org/10.2166/wh.2024.270","url":null,"abstract":"Pseudomonas aeruginosa is a pathogenic bacterium widely distributed in the environment, with increasing concerns about multidrug-resistant (MDR) strains in riverine systems. In this study, we assessed the antibiotic resistance of 50 P. aeruginosa isolates from surface water samples collected at seven distinct sites along the Buriganga River. Antibiotic sensitivity was tested using the Kirby-Bauer Disk Diffusion method. The results showed widespread antibiotic resistance, with 88% of isolates resistant to cefotaxime and tetracycline, followed by 48% resistance to cefepime and 24% to ciprofloxacin. Conversely, most isolates were susceptible to penicillin, aminoglycosides, carbapenems, and fluoroquinolone-class antibiotics, with sensitivity rates of 100, 98, 92, 94, and 96%, respectively. Thirteen isolates (26%) were classified as MDR, predominantly from point-source pollution sites such as industries, medical waste, and municipal waste discharges. Notably, 4% of isolates exhibited resistance to both imipenem and meropenem, raising concerns about the spread of carbapenem-resistant P. aeruginosa in the river. This study highlights the contamination of river water with antibiotic-resistant P. aeruginosa and its potential transmission through aquatic systems. Proper waste management and treatment are critical to controlling the spread of MDR isolates, which pose risks to both public health and the environment.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2358-2369"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Health risk assessment using Monte-Carlo simulations due to arsenic contamination in groundwater in Punjab. 旁遮普地下水砷污染的蒙特卡罗模拟健康风险评估。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-12-03 DOI: 10.2166/wh.2024.188

Sangeeta Yadav, Sunil Kalkal

{"title":"Health risk assessment using Monte-Carlo simulations due to arsenic contamination in groundwater in Punjab.","authors":"Sangeeta Yadav, Sunil Kalkal","doi":"10.2166/wh.2024.188","DOIUrl":"https://doi.org/10.2166/wh.2024.188","url":null,"abstract":"Groundwater contamination is a major environmental concern in many regions of India, including several districts of Punjab. In this study, a comparison is carried out between the deterministic and probabilistic approaches for calculating health hazard parameters due to arsenic contamination in groundwater in India. The probabilistic calculations are carried out through Monte-Carlo simulations to quantify the hazard index (HI) and carcinogenic risk. Sensitivity analysis is carried out to determine the contribution of input variables to the HI. From the deterministic and probabilistic approach, an HI greater than one is obtained in adults for all districts except Moga, whereas, for children, the HI is greater than 1 in all districts. This suggests a higher probability of risks, such as developmental problems and cardiovascular disorders. Excess lifetime carcinogenic risk, a measure of carcinogenic risk, is found above the US Environmental Protection Agency's recommended range of 1 × 10-6 to 1 × 10-4 in all districts. These values clearly indicate the long-term carcinogenic danger associated with arsenic exposure since they are far above the tolerable risk threshold of 1 × 10-4. Thus, necessary mitigation measures should be taken, and routine groundwater monitoring should be performed.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2304-2319"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the drinking water parameters in primary and secondary schools located in the Gaziantep Province Center. 加济安泰普省中心的中小学饮用水参数评价。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.2166/wh.2024.311

Osman Petek, Neriman Aydın

{"title":"Evaluation of the drinking water parameters in primary and secondary schools located in the Gaziantep Province Center.","authors":"Osman Petek, Neriman Aydın","doi":"10.2166/wh.2024.311","DOIUrl":"https://doi.org/10.2166/wh.2024.311","url":null,"abstract":"This cross-sectional study aimed to assess the drinking water parameters in primary and secondary schools. A questionnaire encompassing the schools' general characteristics and information about drinking water was administered to school administrators. Drinking water samples were taken from 60 schools to evaluate drinking water parameters. The data were analyzed using Chi-Square, Mann-Whitney U, and Kruskall-Wallis tests, T-test, and SPSS 22.0 software. Non-compliance with national legislation was observed in 16.7% of schools during drinking water analysis. The microbiological parameters exceeded the threshold values in 90% of the schools where the analysis results of the drinking water were deemed inadequate. The analysis yielded no discernible variations based on school district or type. Mains water was the primary drinking water source in 91.7% of schools. The rate was lower in rural schools compared to urban schools, with a difference of 85 and 95%, respectively. It was found that 41.7% of schools lacked canteens or the sale of packaged water. Consequently, the availability of tap water in schools falls short of the desired standard. It is imperative to investigate the factors leading to microbiological contamination in school drinking water and formulate short-, medium-, and long-term strategies to enhance its safety.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2423-2430"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptosporidium from sheep herds around water tributaries of the Malakand region of Pakistan. 巴基斯坦马拉坎德地区水支流周围羊群中的隐孢子虫。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-11-07 DOI: 10.2166/wh.2024.220

Umar Ali, Naimat Ullah Khan, Kamran Ullah, Tahir Usman, Irfan Khattak, Panagiotis Karanis

{"title":"Cryptosporidium from sheep herds around water tributaries of the Malakand region of Pakistan.","authors":"Umar Ali, Naimat Ullah Khan, Kamran Ullah, Tahir Usman, Irfan Khattak, Panagiotis Karanis","doi":"10.2166/wh.2024.220","DOIUrl":"https://doi.org/10.2166/wh.2024.220","url":null,"abstract":"This study was designed to determine the prevalence of Cryptosporidium infections in sheep herds around tributaries in Pakistan and to evaluate the influence of potential risk factors. In total, 448 samples from 26 sheep herds were screened microscopically and then confirmed through polymerase chain reaction (PCR) amplification of the 18S SSU rRNA gene. Our result revealed that, out of 448 samples, 107 were found positive by microscopy and 77 positive by PCR, with prevalence rates of 23.88% and 17.18% in different breeds of sheep. A high statistical difference exists between the p-value (p <0.05) when comparing diarrheic and non-diarrheic sheep, breeds, ages, genders, and seasons. The highest infection rate was found in the Australian breed (8.25%); season-wise, the highest prevalence was recorded in summer. Furthermore, sheep-secreting diarrheal faces were 41% young sheep and lambs. This reveals that young animals are more susceptible to infection than adults, and cryptosporidial infection decreases with increasing age of the animal. This report is the first on the prevalence of Cryptosporidium in sheep sampled near the tributaries in Pakistan. The implications of this study's findings are clear; immediate action is necessary to prevent further spread and potentially devasting harm.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2320-2330"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and comparative analysis of antimicrobial resistance in Escherichia coli from hospital and municipal wastewater treatment plants. 医院和城市污水处理厂大肠杆菌耐药性特征及比较分析。

IF 2.5 4区环境科学与生态学

Journal of water and health Pub Date : 2024-12-01 Epub Date: 2024-11-21 DOI: 10.2166/wh.2024.126

Gauri Kumar, Keshava Balakrishna, Chiranjay Mukhopadhyay, Vandana Kalwaje Eshwara

{"title":"Characterization and comparative analysis of antimicrobial resistance in Escherichia coli from hospital and municipal wastewater treatment plants.","authors":"Gauri Kumar, Keshava Balakrishna, Chiranjay Mukhopadhyay, Vandana Kalwaje Eshwara","doi":"10.2166/wh.2024.126","DOIUrl":"https://doi.org/10.2166/wh.2024.126","url":null,"abstract":"The spread of antimicrobial resistance (AMR) poses global health threats, with wastewater treatment plants (WWTPs) as hotspots for its development. Horizontal gene transfer facilitates acquisition of resistance genes, particularly through integrons in Escherichia coli. Our study investigates E. coli isolates from hospital and municipal WWTPs, focusing on integrons, their temporal correlation and phenotypic and molecular characterization of AMR. Samples from hospital and municipal WWTPs were collected over two seasons, pre-monsoon (March-May) and post-monsoon (December-February). From the hospital (hWWTP) and municipal (mWWTP) influents, 45 and 172 E. coli isolates were obtained, respectively. E. coli from hWWTP exhibited significantly higher resistance rates than mWWTP to most tested antimicrobials except tetracycline. The hWWTP isolates showed a higher prevalence (86.7%) of multidrug resistance (MDR) compared with mWWTP (48.3%). The proportion of MDR isolates from mWWTP nearly doubled in the post-monsoon season. Integron positivity was 17.7% (hWWTP) and 19.7% (mWWTP) with common gene cassettes conferring resistance to trimethoprim and aminoglycosides. Phylogroup analysis showed a predominance of group A in hWWTP and group B1 in mWWTP. The study highlights the role of hospital and municipal wastewater in disseminating AMR, with high rates of MDR E. coli and class 1 integrons detected.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 12","pages":"2276-2288"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0