Kamila Jessie Sammarro Silva, A. R. Lima, L. D. Dias, Mariana de Souza, Thalita Hellen Nunes Lima, V. Bagnato
{"title":"Hydrogen peroxide preoxidation as a strategy for enhanced antimicrobial photodynamic action against methicillin-resistant Staphylococcus aureus","authors":"Kamila Jessie Sammarro Silva, A. R. Lima, L. D. Dias, Mariana de Souza, Thalita Hellen Nunes Lima, V. Bagnato","doi":"10.2166/wh.2023.245","DOIUrl":"https://doi.org/10.2166/wh.2023.245","url":null,"abstract":"\u0000 \u0000 Antimicrobial photodynamic treatment (aPDT) is a photooxidative process based on the excitation of a photosensitizer (PS) in the presence of molecular oxygen, under specific wavelengths of light. It is a promising method for advanced treatment of water and wastewater, particularly targeting disinfection challenges, such as antibiotic-resistant bacteria (ARB). Research in improved aPDT has been exploring new PS materials, and additives in general. Hydrogen peroxide (H2O2) a widely applied disinfectant, mostly in the food industry and clinical settings, present environmentally negligible residuals at the usually applied concentrations, making it friendly for the water and wastewater sectors. Here, we explored the effects of preoxidation with H2O2 followed by blue light-mediated (450 nm) aPDT using curcumin (a natural-based PS) against methicillin-resistant Staphylococcus aureus (MRSA). Results of the sequential treatment pointed to a slight hampering in aPDT efficiency at very low H2O2 concentrations, followed by an increasing cooperative effect up to a deleterious point (≥7 log10 inactivation in CFU mL–1), suggesting a synergistic interaction of preoxidation and aPDT. The increased performance in H2O2-pretreated aPDT encourages studies of optimal operational conditions for the assisted technology and describes potentials for using the described strategy to tackle the issue of ARB spread.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"54 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138592053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jose Antonio Baz Lomba, João Pires, Mette Myrmel, Jorunn Karterud Arnø, E. Madslien, Petter Langlete, Ettore Amato, S. Hyllestad
{"title":"Effectiveness of environmental surveillance of SARS-CoV-2 as an early-warning system: update of a systematic review during the second year of the pandemic","authors":"Jose Antonio Baz Lomba, João Pires, Mette Myrmel, Jorunn Karterud Arnø, E. Madslien, Petter Langlete, Ettore Amato, S. Hyllestad","doi":"10.2166/wh.2023.279","DOIUrl":"https://doi.org/10.2166/wh.2023.279","url":null,"abstract":"\u0000 \u0000 The aim of this updated systematic review was to offer an overview of the effectiveness of environmental surveillance (ES) of SARS-CoV-2 as a potential early-warning system (EWS) for COVID-19 and new variants of concerns (VOCs) during the second year of the pandemic. An updated literature search was conducted to evaluate the added value of ES of SARS-CoV-2 for public health decisions. The search for studies published between June 2021 and July 2022 resulted in 1,588 publications, identifying 331 articles for full-text screening. A total of 151 publications met our inclusion criteria for the assessment of the effectiveness of ES as an EWS and early detection of SARS-CoV-2 variants. We identified a further 30 publications among the grey literature. ES confirms its usefulness as an EWS for detecting new waves of SARS-CoV-2 infection with an average lead time of 1–2 weeks for most of the publication. ES could function as an EWS for new VOCs in areas with no registered cases or limited clinical capacity. Challenges in data harmonization and variant detection require standardized approaches and innovations for improved public health decision-making. ES confirms its potential to support public health decision-making and resource allocation in future outbreaks.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"51 12","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138593260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kelly Jobling, M. Quintela-Baluja, Francis Hassard, Panagiota Adamou, Adrian Blackburn, Term Research Team, Shannon McIntyre-Nolan, Oscar O'Mara, Jesus Romalde, M. Di Cesare, D. Graham
{"title":"Comparison of gene targets and sampling regimes for SARS-CoV-2 quantification for wastewater epidemiology in UK prisons","authors":"Kelly Jobling, M. Quintela-Baluja, Francis Hassard, Panagiota Adamou, Adrian Blackburn, Term Research Team, Shannon McIntyre-Nolan, Oscar O'Mara, Jesus Romalde, M. Di Cesare, D. Graham","doi":"10.2166/wh.2023.093","DOIUrl":"https://doi.org/10.2166/wh.2023.093","url":null,"abstract":"\u0000 \u0000 Prisons are high-risk settings for infectious disease transmission, including COVID-19, due to their enclosed and semi-enclosed environments. The close proximity between prisoners and staff, and the diversity of prisons reduces the effectiveness of non-pharmaceutical interventions, such as social distancing and clinical testing. Therefore, alternative health monitoring methods, such as wastewater-based epidemiology (WBE), are needed to track pathogens, including SARS-CoV-2. This pilot study assessed WBE to quantify SARS-CoV-2 prevalence in prison wastewater to determine its utility within a health protection system for residents. The study analysed 266 samples from six prisons in England over a 12-week period for nucleoprotein 1 (N1 gene) and envelope protein (E gene) using quantitative reverse transcriptase-polymerase chain reaction. Both gene assays successfully detected SARS-CoV-2 fragments in wastewater samples, with both genes significantly correlating with COVID-19 case numbers across the prisons (p < 0.01). However, in 25% of the SARS-positive samples, only one gene target was detected, suggesting that both genes be used to reduce false-negative results. No significant differences were observed between 14- and 2-h composite samples, although 2-h samples showed greater signal variance. Population normalisation did not improve correlations between the N1 and E genes and COVID-19 case data. Overall, WBE shows considerable promise for health protection in prison settings.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"55 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138606622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adewale Oluwasogo Olalemi, Roseline Atiba, Sally Weston, Guy Howard
{"title":"Sanitary inspection and microbial health risks associated with enteric bacteria in groundwater sources in Ilara-Mokin and Ibule-Soro, Nigeria.","authors":"Adewale Oluwasogo Olalemi, Roseline Atiba, Sally Weston, Guy Howard","doi":"10.2166/wh.2023.111","DOIUrl":"10.2166/wh.2023.111","url":null,"abstract":"<p><p>This study set out to determine the sanitary risk scores and microbial health risks associated with wells and boreholes in Ilara-Mokin and Ibule-Soro, Nigeria. Water samples (n = 96) were collected over a period of five months to determine the levels of enteric bacteria and to perform a Quantitative Microbial Risk Assessment (QMRA) of drinking water quality. Sanitary risk scores revealed `medium' and `low' overall risks for the wells and boreholes, respectively. Three risk factors (faulty fence; small apron; pollution sources) exhibited high significant (p < 0.01) association with the presence of E. coli and thermotolerant coliforms in water samples from the wells. E. coli and Salmonella ranged from 1.82 to 2.28 and 2.15 to 2.63 log<sub>10</sub> CFU/100 ml respectively in water from the wells, but were below detection limit in water from the boreholes. Shigella and Campylobacter were detected in all water samples. Estimated risks of infection associated with Shigella (2.1 × 10<sup>-2</sup> to 2.3 × 10<sup>-1</sup>) were higher than those of Campylobacter (6.7 × 10<sup>-2</sup> to 1.9 × 10<sup>-1</sup>) and Salmonella (1.9×10<sup>-3</sup> to 5.6×10<sup>-3</sup>). Adaption of water safety plans may be advantageous in these settings, since intentional ingestion of water from the wells and boreholes may pose potential risks of diarrheal illness to humans.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"21 12","pages":"1784-1794"},"PeriodicalIF":2.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/wh_2023_111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139048975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Melanie Leopold, Nikola Krlovic, Michael Schagerl, Jakob Schelker, Alexander K T Kirschner
{"title":"Short-term impacts of a large cultural event on the microbial pollution status of a pre-alpine river.","authors":"Melanie Leopold, Nikola Krlovic, Michael Schagerl, Jakob Schelker, Alexander K T Kirschner","doi":"10.2166/wh.2023.232","DOIUrl":"10.2166/wh.2023.232","url":null,"abstract":"<p><p>Rivers are impacted by microbial faecal pollution from various sources. We report on a short-term faecal pollution event at the pre-alpine Austrian river Traisen caused by the large cultural event FM4 Frequency music festival, with around 200,000 visitors over 4 days. We observed a massive increase of the faecal indicator bacteria (FIB) intestinal enterococci during the event, while Escherichia coli concentrations were only slightly elevated. This increase poses a significant potential health threat to visitors and people recreating downstream of the festival area. A plausible explanation for the uncoupling of the two FIBs may have been a differential persistence caused by a combination of factors including water temperature, solar radiation, and the excessive presence of personal care products (PCPs) in the river water. However, a potential impact of PCPs on FIB assay performance cannot be ruled out. Our observations are relevant for other intensively used bathing sites; detailed investigations on persistence and assay performance of the FIB in response to different ingredients of PCPs are highly recommended. We conclude that for future festivals at this river or other festivals taking place under similar settings, a more effective management is necessary to reduce deterioration in water quality and minimise health risks.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"21 12","pages":"1898-1907"},"PeriodicalIF":2.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/wh_2023_232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139048976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kummari Suresh, Devika Pillai, Manju Soman, Akula Sreenivas, Robin Paul
{"title":"Isolation and identification of antimicrobial susceptibility, biofilm formation, efflux pump activity, and virulence determinants in multi-drug resistant Pseudomonas aeruginosa isolated from freshwater fishes.","authors":"Kummari Suresh, Devika Pillai, Manju Soman, Akula Sreenivas, Robin Paul","doi":"10.2166/wh.2023.206","DOIUrl":"10.2166/wh.2023.206","url":null,"abstract":"<p><p>The present study was undertaken to evaluate the prevalence, underlying resistance mechanism, and virulence involved in Pseudomonas aeruginosa (n = 35) isolated from freshwater fishes in Andhra Pradesh, India. Antibiogram studies revealed that 68.5, 62.8, 37.1, 11.4, 8.5, 57.1, 54.2, and 48.5% of isolates had resistance to oxytetracycline, co-trimoxazole, doxycycline, enrofloxacin, ciprofloxacin, cefotaxime, ceftazidime, and ampicillin, respectively. The resistant isolates harboured the tetA (85.7%), tetD (71.4%), tetM (91.4%), sul1 (80%), bla<sub>CTX-M</sub> (57.1%), bla<sub>TEM</sub> (42.8%), and bla<sub>SHV</sub> (48.5%) genes. In total, 50% of the isolates were altered as multi-drug resistant, and the multiple antibiotic resistance index was calculated as 0.4. Furthermore, 37.3, 48.5, and 14.2% of isolates were categorized as strong, moderate, and weak biofilm formers, possessing pslA (91.5%) and pslD (88.6%) biofilm encoding genes. In total, 82.8% of the isolates exhibited efflux pump activity and harboured the mexA (74.2%), mexB (77.1%), and oprM (37.1%) genes. Virulent genes oprL, toxA, exoS, and phzM were detected in 68.5, 68.5, 100, and 17.1% of isolates, respectively. The data suggested that P. aeruginosa harbours multiple resistance mechanisms and virulence factors that may contribute to antibiotic resistance and pathogenicity, and their distribution in fish culture facilities highlights the public health hazards of the food chain.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"21 12","pages":"1858-1870"},"PeriodicalIF":2.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/wh_2023_206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139048974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chemical risks in drinking water of inhabitants in the basin of the Tonle Sap Great Lake","authors":"Kongkea Phan, Sophanith Hoeng, Samnang Phin, Noreaksatya The, Tharith Sriv, Vibol Sao, Chan Oeurn Chey","doi":"10.2166/wh.2023.236","DOIUrl":"https://doi.org/10.2166/wh.2023.236","url":null,"abstract":"Abstract The present study aimed to assess chemical risks in the drinking water of inhabitants in the basin of the Tonle Sap Great Lake. Water samples from tube wells (n = 52), dug wells (n = 13), stored rain water (n = 39), ponds/lakes (n = 19), canals/rivers (n = 24), and household pipe water (n = 45) were collected and analyzed for physicochemical properties, as well as microbial and chemical qualities using standard methods. Analytical results revealed that 42.1% of tube wells had As &gt; 10 μg L−1 while 8.3% had Cr &gt; 0.05 mg L−1. Concurrently, 55.2 and 11.8% of tube wells had Cd &gt; 3.00 μg L–1 and Pb &gt; 10 μg L−1, respectively. Moreover, 35.0% of pipe water had Fe &gt; 0.3 mg L−1, whereas 85.7% of tube wells and 69.2% of dug wells had Mn &gt; 0.1 mg L−1. All water sources including pipe water could pose risks of non-carcinogenic effects of chemical mixtures to all exposure groups through their drinking water pathway. Children were at a higher risk of chemical mixtures in their drinking water than adults. This study suggests that advanced treatment technologies should be applied to the current water treatment plants to provide inhabitants with safe drinking water.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"27 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135042832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
David Sheehan, Phillip Fasham, Rory Moses McKeown, Asoka Jayaratne
{"title":"Development of a tool to support operationalising water safety plans: experiences from a national water utility in Ghana","authors":"David Sheehan, Phillip Fasham, Rory Moses McKeown, Asoka Jayaratne","doi":"10.2166/wh.2023.100","DOIUrl":"https://doi.org/10.2166/wh.2023.100","url":null,"abstract":"Abstract Since their incorporation into the 2004 version of the World Health Organization's (WHO's) Guidelines for Drinking Water Quality (GDWQ), Water Safety Plans (WSPs) continue to be the pre-eminent process for the delivery of safe drinking water to consumers. WSPs achieve this by prioritising proactive, rather than reactive, management of risks to drinking water quality. Since the use of WSPs was incorporated into the GDWQ, a range of supporting resources have been produced to assist water suppliers in preparing WSPs. Producing a robust WSP is an important first step in the management of risk, but in many cases, the implementation of WSPs presents significant challenges, particularly in relation to the implementation of Module 5 (implementing improvements) and Module 6 (monitoring of control measures). To address barriers to WSP implementation, Ghana Water Company Limited (GWCL), in a peer-to-peer partnership with two Australian water utilities, developed a pilot WSP implementation strategy for one of the company's drinking water supply systems. One of the outputs of the collaboration was the development of a framework for operationalising water safety planning, which incorporates basic guidance for embedding the WSP within routine operations, in order to ensure the safe management of drinking water.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"106 45","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135136515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thiago M. Santos, Andrea Wendt, Carolina V. N. Coll, Meghan A. Bohren, Aluisio J. D. Barros
{"title":"<i>E. coli</i> contamination of drinking water sources in rural and urban settings: an analysis of 38 nationally representative household surveys (2014–2021)","authors":"Thiago M. Santos, Andrea Wendt, Carolina V. N. Coll, Meghan A. Bohren, Aluisio J. D. Barros","doi":"10.2166/wh.2023.174","DOIUrl":"https://doi.org/10.2166/wh.2023.174","url":null,"abstract":"Abstract The world is not on track to achieve universal access to safely managed water by 2030, and access is substantially lower in rural areas. This Sustainable Development Goal target and many other global indicators rely on the classification of improved water sources for monitoring access. We aimed to investigate contamination in drinking water sources, comparing improved and unimproved sources in urban and rural settings. We used data from Multiple Indicator Cluster Surveys, which tested samples from the household water source and a glass of water for Escherichia coli contamination across 38 countries. Contamination was widespread and alarmingly high in almost all countries, settings, and water sources, with substantial inequalities between and within countries. Water contamination was found in 51.7% of households at the source and 70.8% in the glass of water. Some improved sources (e.g., protected wells and rainwater) were as likely to be contaminated as unimproved sources. Some sources, like piped water, were considerably more likely to be contaminated in rural than urban areas, while no difference was observed for others. Monitoring water contamination along with further investigation in water collection, storage, and source classification is essential and must be expanded to achieve universal access to safely managed water.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":" 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135285878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria J. Gunnarsdottir, Sigurdur M. Gardarsson, Magnus Eriksson, Hans-Jörgen Albrechtsen, Kim Steve Gerlach Bergkvist, Pekka M. Rossi, Riikka Matilainen, Lisbeth Truelstrup Hansen, Pernille Erland Jensen, Judith Y. A. Maréchal, Mette Myrmel, Frida Celius Kalheim, Kenneth M. Persson, August Bjerkén, Jamie Bartram
{"title":"Implementing risk-based approaches to improve drinking water quality in small water supplies in the Nordic region – barriers and solutions","authors":"Maria J. Gunnarsdottir, Sigurdur M. Gardarsson, Magnus Eriksson, Hans-Jörgen Albrechtsen, Kim Steve Gerlach Bergkvist, Pekka M. Rossi, Riikka Matilainen, Lisbeth Truelstrup Hansen, Pernille Erland Jensen, Judith Y. A. Maréchal, Mette Myrmel, Frida Celius Kalheim, Kenneth M. Persson, August Bjerkén, Jamie Bartram","doi":"10.2166/wh.2023.088","DOIUrl":"https://doi.org/10.2166/wh.2023.088","url":null,"abstract":"Abstract Small water supplies face similar problems worldwide, regardless of ownership or management type. Non-compliance with water quality regulations is more frequent in small supplies than in large ones, as are waterborne disease outbreaks. The new EU Drinking Water Directive requires risk-based approach (RBA) to secure water safety as is recommended in the WHO's Guidelines for drinking water quality through ‘water safety plans’. This is already in regulation in the Nordic countries, although less used in small supplies. In this research, we explore the challenges, barriers and possible solutions to implementing RBA and improving compliance in small supplies. This was achieved by conducting and analysing interviews with 53 stakeholders from all 8 Nordic countries to produce recommendations for action by the different implicated actors. Our findings suggest the centrality of governmental policy, including support for continuous training, provision of simple RBA guidelines and increasing cooperation in the water sector. The Nordic experience reflects global challenges with small water supplies and the trend towards systematic preventive management epitomized in the framework for drinking water safety advocated by the World Health Organization since 2004.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"7 16","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135390800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}