Journal of separation science最新文献_第3页

Stimuli-Responsive Molecularly Imprinted Polymers: Mechanism and Applications 刺激响应分子印迹聚合物：机理与应用》。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-10-09 DOI: 10.1002/jssc.202400441

Zheng Li, Jiaming Deng, Peirong Ma, Haoran Bai, Yuting Jin, Yanling Zhang, Alideertu Dong, Munkhjargal Burenjargal

引用次数: 0

Rapid Characterization of the Potential Active of Sinomenine in Rats by Ultra-High-Performance Liquid Chromatography-Quadrupole-Exactive Orbitrap Mass Spectrometry and Molecular Docking 利用超高效液相色谱-四极杆-精确轨道阱质谱法和分子对接技术快速确定西诺明在大鼠体内的潜在活性特征

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-10-07 DOI: 10.1002/jssc.202400486

Haixia Li, KaiLin Li, Wenhui Cheng, Mingjuan Liu, Linwen Wen, Zexu Zhang, Wendan Zhang, Jin Su, Wei Cai

{"title":"Rapid Characterization of the Potential Active of Sinomenine in Rats by Ultra-High-Performance Liquid Chromatography-Quadrupole-Exactive Orbitrap Mass Spectrometry and Molecular Docking","authors":"Haixia Li, KaiLin Li, Wenhui Cheng, Mingjuan Liu, Linwen Wen, Zexu Zhang, Wendan Zhang, Jin Su, Wei Cai","doi":"10.1002/jssc.202400486","DOIUrl":"10.1002/jssc.202400486","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Sinomenium acutum</i> (Thunb.) Rehd. et Wils is widely used in the treatment of rheumatoid arthritis, with its alkaloid compound sinomenine (SIN) being renowned for its significant anti-inflammatory properties. However, despite its widespread application, the in vivo anti-inflammatory mechanisms and metabolic pathways of SIN remain incompletely understood. This study established a rapid and reliable method based on an ultra-high-performance liquid chromatography method coupled with Quadrupole-Exactive Orbitrap mass spectrometry and molecular docking to identify and characterize SIN and 69 metabolites in rat plasma, urine, and feces, revealing primary metabolic pathways of hydroxylation, demethylation, sulfation, and glucuronidation. Molecular docking results revealed that phase I reactions, including dedimethylation, demethylation, dehydrogenation, and dihydroxylation, along with their composite reactions, were pivotal in influencing SIN's in vivo anti-inflammatory activity. M28, M36, and M59 are potentially the most anti-inflammatory active metabolites of SIN in vivo. This comprehensive analysis unveils SIN's metabolic pathways, offering insights into its biological processes and suggesting a novel approach for exploring active drug constituents. These findings pave the way for further understanding SIN's anti-inflammatory mechanisms, contributing significantly to the development of new therapeutic strategies.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Greening Separation and Purification of Proteins and Peptides 蛋白质和肽的绿化分离与纯化。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-10-07 DOI: 10.1002/jssc.202400554

Katarína Maráková

{"title":"Greening Separation and Purification of Proteins and Peptides","authors":"Katarína Maráková","doi":"10.1002/jssc.202400554","DOIUrl":"10.1002/jssc.202400554","url":null,"abstract":"<div>\u0000 \u0000 <p>The increasing awareness of environmental issues and the transition to green analytical chemistry (GAC) have gained popularity among academia and industry in recent years. One of the principles of GAC is the reduction and replacement of toxic solvents with more sustainable and environmentally friendly ones. This review gives an overview of the advances in applying green solvents as an alternative to the traditional organic solvents for peptide and protein purification and analysis by liquid chromatography (LC) and capillary electrophoresis (CE) methods. The feasibility of using greener LC and CE methods is demonstrated through several application examples; however, there is still plenty of room for new developments to fully realize their potential and to address existing challenges. Thanks to the tunable properties of designer solvents, such as ionic liquids and deep eutectic solvents, and almost infinite possible mixtures of components for their production, it is possible that some new designer solvents could potentially surpass the traditional harmful solvents in the future. Therefore, future research should focus mainly on developing new solvent combinations and enhancing analytical instruments to be able to effectively work with green solvents.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Microbial Phospholipase and Lipase Activity Through the Chromatographic Analysis of Crude, Degummed, and Transesterified Soybean Oil for Biodiesel Production 通过对用于生物柴油生产的粗豆油、脱胶豆油和酯交换豆油进行色谱分析，评估微生物磷脂酶和脂肪酶的活性。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-10-07 DOI: 10.1002/jssc.202400325

Ahlem Eddehech, Alessia Tropea, Francesca Rigano, Danilo Donnarumma, Emna Ben Abdallah, Francesco Cacciola, Luigi Mondello, Zied Zarai

{"title":"Evaluation of Microbial Phospholipase and Lipase Activity Through the Chromatographic Analysis of Crude, Degummed, and Transesterified Soybean Oil for Biodiesel Production","authors":"Ahlem Eddehech, Alessia Tropea, Francesca Rigano, Danilo Donnarumma, Emna Ben Abdallah, Francesco Cacciola, Luigi Mondello, Zied Zarai","doi":"10.1002/jssc.202400325","DOIUrl":"10.1002/jssc.202400325","url":null,"abstract":"<p>The present study aimed at synthesizing fatty acid methyl esters in a combined enzymatic method by applying degumming and transesterification of soybean oil. A soluble lipase from <i>Serratia</i> sp. W3 and a recombinant phosphatidylcholine-preferring phospholipase C (PC-PLC) from <i>Bacillus thuringiensis</i> were used in a consecutive manner for phosphorus removal and conversion into methyl esters. By applying 1% of recombinant PC-PLC almost 83% of phosphorus was removed (final content of 21.01 mg/kg). Moreover, a sensitive and selective high-performance liquid chromatography method coupled to tandem mass spectrometry was applied to obtain a comprehensive lipid profile for the simultaneous evaluation of phospholipids removal and diacylglycerol (DAG) increase. A significant increase for all the monitored DAG species, up to 138.42%, was observed by using the enzymatic degumming, in comparison to the crude sample, resulting in an increased oil yield. <i>Serratia</i> sp. W3 lipase was identified as a suitable biocatalyst for biodiesel production, converting efficiently the acylglycerols. The results regarding the physical-chemical characteristics show that the cetane level, density and pour point of the obtained biodiesel are close to current regulation requirements. These findings highlight the potential of a two-step process implementation, based on the combination of lipase and phospholipase, as a suitable alternative for biodiesel production.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400325","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an Untargeted Method for the Analysis of the Volatile Profile of Onions via Solid-Phase Microextraction Arrow-Headspace-Gas Chromatography-Mass Spectrometry Using Design of Experiments 利用实验设计，开发一种通过固相微萃取箭头空间-气相色谱-质谱法分析洋葱挥发性成分的非目标方法。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-10-03 DOI: 10.1002/jssc.202400305

Lea Böckstiegel, Theresa Schaumann, Björn Egert, Sabine E. Kulling, Christoph H. Weinert

{"title":"Development of an Untargeted Method for the Analysis of the Volatile Profile of Onions via Solid-Phase Microextraction Arrow-Headspace-Gas Chromatography-Mass Spectrometry Using Design of Experiments","authors":"Lea Böckstiegel, Theresa Schaumann, Björn Egert, Sabine E. Kulling, Christoph H. Weinert","doi":"10.1002/jssc.202400305","DOIUrl":"10.1002/jssc.202400305","url":null,"abstract":"<p>The distinctive aroma of onions, consisting primarily of sulfur-containing compounds, is one of the reasons for the popularity of the vegetable. The rapid enzymatic and chemical reactions that occur after the destruction of onion bulb tissue render the analysis of the volatile profile challenging. Therefore, sample preparation is a crucial step in the analysis of the onion volatilome, but it often does not receive the necessary attention in the literature. In this work, we focused on two aspects: Firstly, we compared different sample preparation approaches to maximize the volatile yield and to enable the description of the onion volatile profile as it would emerge after a solid-phase microextraction (SPME) Arrow sampling procedure. For headspace (HS)-gas chromatography-mass spectrometry analysis, onion juice with the addition of an ammonium sulfate solution proved to be the best option. Secondly, we optimized the HS sampling and desorption parameters (enrichment time, enrichment temperature, agitator speed, desorption time) for onion volatiles using the efficient design of experiments (DoE) approach. The optimal conditions for the analysis with HS-SPME Arrow were an enrichment time of 75 min at 60°C with an agitator speed of 713 rpm and a desorption time of 120 s.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preconcentration of Cinnamic Acid Derivatives in Traditional Chinese Medicines by an Effective Dispersive Liquid-Liquid Microextraction Based on a Hydrophobic Deep Eutectic Solvent 基于疏水性深共晶溶剂的有效分散液-液微萃取技术预富集中药中的肉桂酸衍生物

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-10-01 DOI: 10.1002/jssc.202400363

Qian Li, Li-mei Wang, Shu-mei Zhang, Guang-bin Zhang, Shuang Hu

{"title":"Preconcentration of Cinnamic Acid Derivatives in Traditional Chinese Medicines by an Effective Dispersive Liquid-Liquid Microextraction Based on a Hydrophobic Deep Eutectic Solvent","authors":"Qian Li, Li-mei Wang, Shu-mei Zhang, Guang-bin Zhang, Shuang Hu","doi":"10.1002/jssc.202400363","DOIUrl":"10.1002/jssc.202400363","url":null,"abstract":"<div>\u0000 \u0000 <p>A dispersive liquid-liquid microextraction based on hydrophobic deep eutectic solvent (hDES) was developed for the extraction and quantification of four cinnamic acid derivatives in traditional Chinese medicines coupled with high-performance liquid chromatography-ultraviolet detection. In this method, a hDES (tetrabutylammonium chloride-hexanoic acid, molar ratio of 1:2) was prepared as the extractant. It only took 15 s to handle multiple samples simultaneously by hand-assisted dispersion. The use of a narrow-bore tube reduced the amount of the hydrophobic extractant with easier recovery. The approach was influenced by several key parameters, including the composition and consumption of the DES, sample phase pH, salt amount, extraction time, and centrifugation time, all of which had been investigated and optimized. Moreover, the formation of the DES was characterized by Fourier-transform infrared spectroscopy and differential scanning calorimetry. Under the optimal conditions, enrichment factors of the target analytes ranged from 135 to 220. Satisfactory linearities (<i>r</i> ≥ 0.9977), detection limits (0.2–0.4 ng/mL), precision (<8.5%), and accuracy (recoveries: 90.0%–104.6%) were obtained. The method has been successfully applied to the simultaneous extraction and preconcentration of four cinnamic acid derivatives in Chinese medicinal samples with rapidness, high efficiency, and convenience.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification, Source Exploration, and Bioactivity Evaluation of Oligosaccharides in Sijunzi Decoction 四君子汤中低聚糖的纯化、探源及生物活性评价

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-28 DOI: 10.1002/jssc.202400407

Xiaonan Chen, Zongjin Pu, Yixin Liu, Bangjian Dong, Xiaobo Li

{"title":"Purification, Source Exploration, and Bioactivity Evaluation of Oligosaccharides in Sijunzi Decoction","authors":"Xiaonan Chen, Zongjin Pu, Yixin Liu, Bangjian Dong, Xiaobo Li","doi":"10.1002/jssc.202400407","DOIUrl":"10.1002/jssc.202400407","url":null,"abstract":"<div>\u0000 \u0000 <p>Sijunzi decoction (SJZD) has been widely used to treat splenic deficiency syndrome. Previous studies confirmed that polysaccharides and non-polysaccharides (NPS) are the main active components of SJZD. This study aimed to investigate the composition and activity of oligosaccharides in NPS. In this study, the oligosaccharide component (named SJZD-OGS), consisting of several different oligosaccharides, was separated and purified from non-polysaccharides of SJZD (SJZD_NPS). Ultra-high-performance liquid chromatography–ion mobility spectrometry–quadrupole time-of-flight mass spectrometry (UHPLC–IMS–QTOF/MS), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) were used to analyze SJZD-OGS. SJZD-OGS, with a molecular weight of 2178 Da, was composed of glucose and fructose, and its chemical composition mainly included fructo-oligosaccharides (FOS) with a degree of polymerization of 3–14 (DP 3–14). The results of oligosaccharide source exploration demonstrated that the chemical composition of SJZD-OGS was different from that of the oligosaccharides of each herb in SJZD. In addition, SJZD-OGS showed good anti-inflammatory activity on macrophage cells and intestinal epithelial cell protective activity. The present work provides experimental evidence for elucidating the pharmaceutical components of SJZD and presents an effective strategy for the research of oligosaccharides in traditional Chinese medicine (TCM).</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Polymer-Based Polar Stationary Phase Grafted With Modified Lysine for Hydrophilic Interaction Chromatography 接枝了改性赖氨酸的聚合物型极性固定相，用于亲水相互作用色谱法

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-25 DOI: 10.1002/jssc.202400521

Yufeng Shen, Rui Jin, Feifang Zhang, Bingcheng Yang

引用次数: 0

Facile Synthesis of Aptamer-Functionalized Polydopamine-Coated Magnetic Graphene Oxide Nanocomposites for Highly Efficient Purification of His-Tagged Proteins 用于高效纯化 His 标记蛋白质的 Aptamer 功能化聚多巴胺包覆磁性石墨烯氧化物纳米复合材料的简便合成方法

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-25 DOI: 10.1002/jssc.202400471

Qian Qin, Xiaolong Liu, Xun Wang, Lina Zhou, Huihui Wan, Qingxin Yin, Di Chen

{"title":"Facile Synthesis of Aptamer-Functionalized Polydopamine-Coated Magnetic Graphene Oxide Nanocomposites for Highly Efficient Purification of His-Tagged Proteins","authors":"Qian Qin, Xiaolong Liu, Xun Wang, Lina Zhou, Huihui Wan, Qingxin Yin, Di Chen","doi":"10.1002/jssc.202400471","DOIUrl":"https://doi.org/10.1002/jssc.202400471","url":null,"abstract":"<div>\u0000 \u0000 <p>Recombinant proteins hold significant importance in numerous disciplines. As the demand for expressing and purifying these proteins grows, the scientific community is in dire need of a simple yet versatile methodology that can efficiently purify these proteins. Aptamers as synthetic nucleic acid–based ligands with high affinity have shown promise in this regard, as they can capture targets through molecular recognition. In this study, novel aptamer-functionalized polydopamine-coated magnetic graphene oxide nanocomposites were facilely prepared, achieving an impressive average aptamer coverage density (45 nmol/mg). These nanocomposites exhibited a uniform structure and robust magnetic responsiveness. The findings indicated that they possess several advantages, such as rapid adsorption, substantial capacity (171.4 mg/g), and excellent reusability. Notably, due to the inherent properties of nucleic acids, the immobilized aptamer-magnetic beads can be utilized repeatedly with high purification efficiency. Finally, the nanocomposites were further employed to purify His-tagged proteins from actual samples. Remarkably, they were able to selectively and efficiently isolate His-tagged retinoid X receptor alpha protein from complex <i>Escherichia coli</i> lysate. The purified His-tagged retinoid X receptor alpha protein was analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. This confirmed the efficacy of developed nanocomposites, reinforcing their vast potential for purification of His-tagged recombinant proteins.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a Soft Ionization by Chemical Reaction in Transfer Ion Source Hyphenated With Supercritical Fluid Chromatography-Tandem Mass Spectrometry 超临界流体色谱-串联质谱联用转移离子源中化学反应软电离的表征

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-25 DOI: 10.1002/jssc.202400500

Niray Bhakta, Alexander S. Kaplitz, Destini Black, Kevin A. Schug

{"title":"Characterization of a Soft Ionization by Chemical Reaction in Transfer Ion Source Hyphenated With Supercritical Fluid Chromatography-Tandem Mass Spectrometry","authors":"Niray Bhakta, Alexander S. Kaplitz, Destini Black, Kevin A. Schug","doi":"10.1002/jssc.202400500","DOIUrl":"https://doi.org/10.1002/jssc.202400500","url":null,"abstract":"<div>\u0000 \u0000 <p>A commercially available dielectric barrier discharge ionization (DBDI) source was tested with supercritical fluid chromatography-mass spectrometry (SFC-MS). The compound mixture investigated comprised caffeine, theobromine, theophylline, uracil, testosterone, and pyrene, diluted in methanol. Dynamic response ranges were evaluated with multiple injections at different concentrations. Precision studies demonstrated the robustness and sensitivity of the ionization source across a concentration range of 10–1000 ng/mL. Results from this experiment showed linear regression of 0.99 or greater for all analytes tested over the range with a relative standard deviation (RSD) of less than 10% down to 10 ng/mL for all analytes except theobromine, which had an RSD of less than 10% down to 25 ng/mL. Notably, this study marks the first investigation of sensitivity for coupling a commercial DBDI source with SFC; a limit of detection less than 1 ng/mL was achieved for all compounds. This study demonstrates chromatographic separation by SFC and MS analysis for compounds that ionize poorly using traditional atmospheric pressure ionization, such as polycyclic aromatic hydrocarbons. Combining SFC with the DBDI source opens promising avenues for analyzing compounds that were previously challenging to characterize with standard atmospheric pressure ionization techniques.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0