Journal of separation science最新文献_第2页

Development of a Capsule Phase Microextraction Protocol for the Determination of Monomers Released from Dental Resins in Cola Drinks Analyzed by High-Pressure Liquid Chromatography Coupled to UV 高压液相色谱-紫外耦合法测定可乐饮料中口腔树脂中单体的胶囊相微萃取方法的建立

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-26 DOI: 10.1002/jssc.70183

Magdalini Vladitsi, Natasa P. Kalogiouri, Petros Mourouzis, Abuzar Kabir, Kenneth Furton, Victoria F. Samanidou

{"title":"Development of a Capsule Phase Microextraction Protocol for the Determination of Monomers Released from Dental Resins in Cola Drinks Analyzed by High-Pressure Liquid Chromatography Coupled to UV","authors":"Magdalini Vladitsi, Natasa P. Kalogiouri, Petros Mourouzis, Abuzar Kabir, Kenneth Furton, Victoria F. Samanidou","doi":"10.1002/jssc.70183","DOIUrl":"https://doi.org/10.1002/jssc.70183","url":null,"abstract":"<p>A novel, simple capsule phase microextraction combined with high-pressure liquid chromatography coupled with ultraviolet detection analytical method was developed for the simultaneous determination of organic monomers that can be released from dental resins [bisphenol-A (BPA), bisphenol A glycerolatedimethacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), and urethane dimethacrylate (UDMA)] in cola drinks. The critical parameters that affect the extraction, such as the selection of the capsule type and size, extraction time, type, and volume of elution solvent, stirring rate during the extraction, and desorption time, were optimized. The method was subsequently validated in terms of selectivity, linearity, accuracy, precision, and robustness and showed satisfactory results. The limits of detection and quantitation were equal to 0.06 and 0.2 ng/µL, respectively, and the recoveries ranged between 93.3% and 106.9%. The method was employed in the determination of monomers released from cola drinks. For this purpose, cola refreshments were incubated with dental resins for 24 h and 7 days, and the proposed method was successfully used for monitoring the release in cola samples.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70183","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on Adsorption of Two Functionalized Conjugated Microporous Polymers: Extraction of Carbendazim and Thiabendazole in Ophiopogon japonicus 两种功能化共轭微孔聚合物对麦冬中多菌灵和噻苯达唑的吸附研究

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-26 DOI: 10.1002/jssc.70185

Hechen Liu, Chongzhi Wang, Na Li, Baokun Tang, Tao Zhu

{"title":"Study on Adsorption of Two Functionalized Conjugated Microporous Polymers: Extraction of Carbendazim and Thiabendazole in Ophiopogon japonicus","authors":"Hechen Liu, Chongzhi Wang, Na Li, Baokun Tang, Tao Zhu","doi":"10.1002/jssc.70185","DOIUrl":"https://doi.org/10.1002/jssc.70185","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, two novel conjugated microporous polymers modified with functional groups were synthesized. Meanwhile, static and dynamic adsorption tests were conducted on the obtained materials. To achieve the best extraction efficiency, the parameters of the pipette-tip solid-phase extraction procedure were optimized, including the amount of adsorbent, the type and amount of washing solvent, and the elution solvent. The results demonstrate that the adsorption performance of the sulfonated conjugated microporous polymer is remarkably superior to that of the aminated conjugated microporous polymer. Under the optimal conditions, the linear range for targets was 0.05–10.00 µg/g using this method, the limits of detection were 0.009 µg/g for carbendazim and 0.004 µg/g for thiabendazole, and the recoveries were in the range of 80.7%–108.3% for carbendazim and 87.4%–119.2% for thiabendazole, which was successfully applied to the analysis of target residues in actual samples. In addition, the adsorption capacity of material prepared in this study was superior to that of several commercially available adsorbents. Therefore, a method for the determination of target residues in <i>Ophiopogon japonicus</i> by pipette-tip solid-phase extraction coupled with high-performance liquid chromatography using the material adsorbent was established. This method is important for detecting target residues.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Identification of Chemical Constituents in Qubai Babuqi Tablets by Ultra-High-Performance Liquid Chromatography Quadrupole-Orbitrap-Mass Spectrometry 超高效液相色谱-四极杆-轨道-质谱法快速鉴定祛白八步芪片中的化学成分

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-22 DOI: 10.1002/jssc.70117

Xuebin Qin, Jiahui Wang, Lulu Yu, Miao Xu, Siyue Chen, Yajie Dou, Guangzhi Cui, Yuming Wang, Yanyan Xu, Yubo Li

{"title":"Rapid Identification of Chemical Constituents in Qubai Babuqi Tablets by Ultra-High-Performance Liquid Chromatography Quadrupole-Orbitrap-Mass Spectrometry","authors":"Xuebin Qin, Jiahui Wang, Lulu Yu, Miao Xu, Siyue Chen, Yajie Dou, Guangzhi Cui, Yuming Wang, Yanyan Xu, Yubo Li","doi":"10.1002/jssc.70117","DOIUrl":"https://doi.org/10.1002/jssc.70117","url":null,"abstract":"<div>\u0000 \u0000 <p>Qubai Babuqi Tablets (QBBBQT) is a traditional Chinese medicine compound preparation. It is mainly used in treating vitiligo, with the effect of activating pulse-beat and regulating blood condition. However, the chemical composition of QBBBQT has not been reported, and it was difficult to determine its active and toxic components. In this study, a simple and efficient method was established to characterize the chemical constituents of the alcohol extract of QBBBQT. Which used ultra-high-performance liquid chromatography quadrupole-orbitrap-mass spectrometry to match experimental MS<sup>2</sup> with characteristic ions and neutral losses for rapid analysis and identification of its chemical composition. Finally, a total of 106 chemical components were identified, including 47 flavonoids, 13 coumarins, 21 organic acids, 11 diarylheptanes, four saccharides, and 10 others. This method can be used for the systematic and rapid analysis of the chemical components in QBBBQT, which is of great significance for the study of its pharmacodynamic material basis and quality control.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Determination of 10 Fat-Soluble Vitamins by Ultra-Performance Liquid Chromatography in Multivitamins With Minerals Capsules 超高效液相色谱法同时测定矿物质复合维生素胶囊中10种脂溶性维生素的含量

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-22 DOI: 10.1002/jssc.70167

Salika Jeelani, Zarema Kassymbek

{"title":"Simultaneous Determination of 10 Fat-Soluble Vitamins by Ultra-Performance Liquid Chromatography in Multivitamins With Minerals Capsules","authors":"Salika Jeelani, Zarema Kassymbek","doi":"10.1002/jssc.70167","DOIUrl":"https://doi.org/10.1002/jssc.70167","url":null,"abstract":"<p>A novel and accurate ultra-high performance liquid chromatography (UHPLC) method combined with UV detector was developed for the simultaneous determination of 10 fat-soluble vitamins (retinyl acetate, retinyl palmitate, beta carotene, alpha tocopherol, alpha tocopheryl acetate, alpha tocopheryl acid succinate, ergocalciferol, cholecalciferol, phytonadione, and menaquinone-7) in multivitamin with mineral capsules. The chromatographic separation was achieved on a Waters XBridge Sheild C18 (100 × 2.1 mm, 1.7 µm) column. The mobile phase comprised of 0.1% formic acid (v/v) in water and 0.1% formic acid in acetonitrile (v/v) delivered in a gradient mode. An enhanced and simple sample preparation procedure involving enzyme digestion of the gel coating of the capsules and ultra-sonication was developed compared to the complex and time-consuming saponification methods. The method was validated to fulfill International Conference on Harmonization (ICH) requirements and included specificity, linearity, accuracy, precision, and robustness. The linearity of the method was excellent (<i>R</i><sup>2</sup> > 0.999), the RSD for the precision was < 5% and the recovery of the vitamins was in the range of 99.2%–101.9%, demonstrating that the method is suitable for analysis of fate-soluble vitamins in multivitamin capsules. The developed method could be incorporated into the USP-NF Multivitamin with minerals capsules monograph.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous Depletion of Tetrahydrocannabinol From Cannabis Extract Through Simulated Moving Bed Chromatography Using Green Mobile Phase 绿色流动相模拟移动床色谱法连续萃取大麻提取物中的四氢大麻酚

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-22 DOI: 10.1002/jssc.70175

Greta Compagnin, Chiara De Luca, Chiara Nosengo, Giorgia Greco, Martina Catani, Alberto Cavazzini, Yannick Krauke, Simona Felletti

{"title":"Continuous Depletion of Tetrahydrocannabinol From Cannabis Extract Through Simulated Moving Bed Chromatography Using Green Mobile Phase","authors":"Greta Compagnin, Chiara De Luca, Chiara Nosengo, Giorgia Greco, Martina Catani, Alberto Cavazzini, Yannick Krauke, Simona Felletti","doi":"10.1002/jssc.70175","DOIUrl":"https://doi.org/10.1002/jssc.70175","url":null,"abstract":"<p>The interest toward greener and more sustainable chemical processes is constantly growing, especially in chemical industries, where there is an urgent need to reduce the waste on one hand, and to replace not environmentally friendly organic solvents, on the other.</p><p>One of the current challenges in industrial processes is the depletion of the psychoactive cannabinoid tetrahydrocannabinol (THC) from hemp extracts. Indeed, current legislations impose that THC in hemp products must meet defined concentration limits or, in some cases, it must be completely removed. In the present study, the continuous depletion of THC has been performed through simulated moving bed chromatography operated with a fully green mobile phase, made of water and ethanol. This process permits to overcome the limits and drawbacks of available purification methods, based on toxic solvents and traditional single column chromatography.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Materials Institute Lavoisier-68(Al)-coated Frosted Glass Rods for Enhanced Detection of Tetracyclines in Food Samples by Suspended Bar Sorptive Extraction 拉瓦锡-68（Al）涂层磨砂玻璃棒悬浮棒吸附萃取强化检测食品样品中的四环素

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-22 DOI: 10.1002/jssc.70141

Tao Bao, Jiao Long, Jina Wang, Meng Sun, Xinglong Zheng, Sicen Wang

{"title":"Materials Institute Lavoisier-68(Al)-coated Frosted Glass Rods for Enhanced Detection of Tetracyclines in Food Samples by Suspended Bar Sorptive Extraction","authors":"Tao Bao, Jiao Long, Jina Wang, Meng Sun, Xinglong Zheng, Sicen Wang","doi":"10.1002/jssc.70141","DOIUrl":"https://doi.org/10.1002/jssc.70141","url":null,"abstract":"<div>\u0000 \u0000 <p>Herein, we present a sensitive analytical method utilizing Materials Institute Lavoisier (MIL)-68(Al)-coated frosted glass rods (MIL-68(Al)@FGR) coupled with ultra-high-performance liquid chromatography for detecting trace tetracycline (TC) antibiotics in food matrices. The synthesized coating was thoroughly characterized through field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy analyses. Systematic optimization of critical parameters, including extraction time, sample pH, formic acid concentration, and desorption time achieved optimal extraction efficiency. The developed method exhibited a wide linear range (3–70 ng/mL) with low detection limits (0.1–0.3 ng/mL) for target analytes. Practical application to spiked food samples demonstrated satisfactory recoveries of 75.2%–115.1% across milk, chicken, and egg matrices, accompanied by precise relative standard deviations of ≤8.4%. The MIL-68(Al)@FGR-based approach shows exceptional resistance to matrix interference and detection sensitivity, establishing an effective strategy for monitoring TC residues in complex food samples.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption of Rubidium/Cesium From Aqueous Solution Using Silane-Modified Prussian Blue-Based Composites 硅烷改性普鲁士蓝基复合材料对水溶液中铷/铯的吸附研究

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-19 DOI: 10.1002/jssc.70164

Mengmeng Wang, Jian Xu, Liang Zhao, Chenxiao Lin, Jie Gao, Yonggao Xia

{"title":"Adsorption of Rubidium/Cesium From Aqueous Solution Using Silane-Modified Prussian Blue-Based Composites","authors":"Mengmeng Wang, Jian Xu, Liang Zhao, Chenxiao Lin, Jie Gao, Yonggao Xia","doi":"10.1002/jssc.70164","DOIUrl":"https://doi.org/10.1002/jssc.70164","url":null,"abstract":"<div>\u0000 \u0000 <p>This study presents the synthesis and application of a novel silane-modified Prussian blue-based composite (PB-Si) adsorbent for the efficient extraction of rubidium and cesium ions from aqueous solutions. The PB-Si adsorbent is synthesized using the Pickering high internal phase emulsions polymerization method, which leverages the stability of Pickering emulsions formed by nanoparticles at the interface of two immiscible solutions. The modification of Prussian blue with 3-aminopropyl trimethoxy silane enhances the hydrophilicity and stability of the adsorbent. Adsorption kinetics and isotherms results reveal that the PB-Si adsorbent achieves rapid adsorption equilibrium within 2.5–3 h. The PB-Si adsorbent exhibits high adsorption capacities of 317.4 mg/g for Rb<sup>+</sup> and 402.6 mg/g for Cs<sup>+</sup>. In addition, the developed adsorbent retains 88.3% and 91.7% of its initial adsorption capacity for Rb<sup>+</sup> and Cs<sup>+</sup>, respectively, after five consecutive adsorption–desorption cycles. The investigation into the adsorption mechanism indicates that chemisorption, driven by electrostatic attraction and chemical complexation with functional groups (─COO<sup>−</sup>) on the surface of the adsorbent, is the dominant process. This study highlights the potential of the PB-Si composite adsorbent as an effective and regenerable material for the extraction of Rb<sup>+</sup> and Cs<sup>+</sup> from aqueous solutions, with implications for environmental remediation and resource recovery.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Analytical Quality by Design Approaches on the Development and Optimization of Size Exclusion Chromatography Analytical Procedures for Bovine Serum Albumin 设计法评价牛血清白蛋白色谱分析方法的质量

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-15 DOI: 10.1002/jssc.70168

Alessio Gaggero, Dalibor Jeremic, Reingard Sattler, Amrit Paudel, Jesús Alberto Afonso Urich

{"title":"Evaluation of Analytical Quality by Design Approaches on the Development and Optimization of Size Exclusion Chromatography Analytical Procedures for Bovine Serum Albumin","authors":"Alessio Gaggero, Dalibor Jeremic, Reingard Sattler, Amrit Paudel, Jesús Alberto Afonso Urich","doi":"10.1002/jssc.70168","DOIUrl":"https://doi.org/10.1002/jssc.70168","url":null,"abstract":"<div>\u0000 \u0000 <p>The development of analytical procedures, following the International Council of Harmonisation (ICH) regulatory requirements, is crucial for managing the lifecycle of methodologies. This study applies analytical quality by design (AQbD) principles to optimize size exclusion chromatography (SEC) determination for bovine serum albumin (BSA), chosen as a model drug. Key method variables, including mobile phase buffer concentration, flow rate, and column type, were systematically evaluated using risk assessment and design of experiments (DoE). A consistent and rigorous workflow was applied to prove and ensure the robustness of this methodology. The SEC method was established through a framed analytical target profile (ATP), complying with chromatography parameters like tailing, resolution, capacity, and sensitivity. Those were achieved with a Waters XBridge BEH200A SEC column (200 Å, 3.5 µm, 7.8 mm × 300 mm) at 20°C, using a phosphate buffer saline (PBS) solution at pH 7.4 in isocratic elution with a flow of 0.3 mL/min and UV detection of 280 nm. The method was validated according to ICH Q2 (<i>R</i><sup>2</sup>) and Q14 guidelines. The linear response was demonstrated over concentration ranges of 100 µg/mL and 10 mg/mL (<i>R</i><sup>2</sup> = 0.9998), and desirable standards of accuracy and precision were met. Although this work highlights the benefits of AQbD in developing trustworthy analytical methods, SEC constitutes a challenge for applying these principles considering the short lifetime of the column, reproducibility, and sensitivity.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatographic Techniques for Improving the LC/MS Quantification of PFAS

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-13 DOI: 10.1002/jssc.70155

Stephan Buckenmaier, Christina Riemenschneider, Alexander Schächtele, Susanne Sölter

{"title":"Chromatographic Techniques for Improving the LC/MS Quantification of PFAS","authors":"Stephan Buckenmaier, Christina Riemenschneider, Alexander Schächtele, Susanne Sölter","doi":"10.1002/jssc.70155","DOIUrl":"https://doi.org/10.1002/jssc.70155","url":null,"abstract":"<div>\u0000 \u0000 <p>The liquid chromatography / mass spectrometry (LC/MS) quantification of per- and polyfluoroalkyl substances (PFAS) in complex matrices presents challenges. Despite advanced sample preparation techniques to isolate analytes, residual matrix components can still interfere with MS detection. Additionally, extracts often contain high levels of organic solvents limiting the volume that can be injected when using reversed-phase HPLC. Yet, increasing the injection volume could be a simple path to lower detection limits. Feeding PFAS samples, dissolved in pure acetonitrile, at controlled speed into the stream of mobile phase resulted in an analyte focusing effect on the column. By using this approach, the injection volume could be increased 10 times compared to the conventional injection mode, as did the detection sensitivity. Feed injection was coupled to two-dimensional liquid chromatography (2D-LC) in a configuration in which the same triple quadrupole MS could be used for detection in both separation dimensions. The improved separation markedly reduced ion suppression effects and allowed to distinguish the critical PFAS compound perfluorobutanoic acid (PFBA) from isomeric matrix components within tomato extracts interfering with its quantification when only LC/MS was used. This 2D-LC/MS approach therefore fulfilled recommendations that PFBA should be analyzed using a secondary LC approach, without adding a marked amount of time to the analysis.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short Review on Currently Used Sample Preparation and Determination Methods of Risdiplam 利司地泮样品制备及测定方法综述

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-05-12 DOI: 10.1002/jssc.70173

Natalia Balińska, Karolina Ostrowska, Sylwia Studzińska

{"title":"Short Review on Currently Used Sample Preparation and Determination Methods of Risdiplam","authors":"Natalia Balińska, Karolina Ostrowska, Sylwia Studzińska","doi":"10.1002/jssc.70173","DOIUrl":"https://doi.org/10.1002/jssc.70173","url":null,"abstract":"<div>\u0000 \u0000 <p>Risdiplam is a new therapeutic agent developed to treat spinal muscular atrophy (SMA), a genetic neurodegenerative disease caused by mutations in the <i>SMN1</i> gene. Unlike previous invasive therapies, risdiplam offers the advantage of oral administration, significantly improving patient comfort and accessibility. The review provides information on an SMA historical overview, breakthrough therapies of the development, and design of the methods used to treat SMA. We then focus on its structure and physicochemical properties. The analysis of risdiplam concentrates on developing improved analytical methods for the precise quantification of risdiplam and its metabolites by high-performance liquid chromatography with mass spectrometry in biological samples using octadecyl stationary phases. For sample preparation, only the protein precipitation method was used. Challenges associated with the risdiplam analytics include developing a highly sensitive and selective method in biological matrices and dealing with potential interferences from the biological matrix. Future research should focus on improving analytical methods, investigating metabolite activity, and expanding our knowledge of its long-term effects.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 5","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0