Journal of separation science最新文献

Primaquine Analysis in Pharmaceutical Formulation Using Multiple and Short-End Injections by Capillary Zone Electrophoresis-Ultraviolet Detection 利用毛细管区带电泳-紫外检测法，通过多次和短端注射分析药物制剂中的普利马喹。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-18 DOI: 10.1002/jssc.70026

Jéssica Cordeiro Queiroz de Souza, Paula Rocha Chellini, Marcus Vinícius Nora de Souza, Marcone Augusto Leal de Oliveira

{"title":"Primaquine Analysis in Pharmaceutical Formulation Using Multiple and Short-End Injections by Capillary Zone Electrophoresis-Ultraviolet Detection","authors":"Jéssica Cordeiro Queiroz de Souza, Paula Rocha Chellini, Marcus Vinícius Nora de Souza, Marcone Augusto Leal de Oliveira","doi":"10.1002/jssc.70026","DOIUrl":"10.1002/jssc.70026","url":null,"abstract":"<div>\u0000 \u0000 <p>Novel methods were proposed for determining primaquine (PQN) in tablets by multiple-injection capillary zone electrophoresis (MI-CZE) and by short-end injection CZE (SEI-CZE), both with ultraviolet detection. The background electrolyte (BGE), consisting of 20 mmol/L of tris (hydroxymethyl) aminomethane and 30 mmol/L of hydrochloric acid at pH 2.0, was selected considering a comprehensive study involving the effective mobility versus pH curves of the analytes and BGE components. Experimental designs were applied in methods developments, showing the chemometric tool's applicability in achieving suitable electrophoretic conditions. A baseline resolution in the separation of adjacent peak pairs was obtained by injecting a spacer electrolyte for 18 s, with a voltage of + 15 kV, and the sample can be injected six consecutive times in a single run in less than 3 min, in the MI-CZE method. For the SEI-CZE method, the migration time of PQN was 0.6 min, and the method was applied to a demonstrative forced degradation study. Some validation parameters were evaluated for both methods, and all results were satisfactory, indicating that they can be implemented as PQN determination methods in routine quality control analyses.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 22","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality Assessment of Corydalis saxicola Bunting Using Quantitative Analysis of Multi-Components by Single Marker and Fingerprint Analysis 利用单一标记和指纹分析法对多种成分进行定量分析，评估 Corydalis saxicola Bunting 的质量。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-14 DOI: 10.1002/jssc.70028

Yuanle Shen, Xu Yang, Hongli Feng, Huini Ruan, Yuan Zhou, Zihong Li, Jilang Li, Peng Zhang, Kaitong Li, Honglan Wang, Shen Liu, Xiaoxiu Shi, Jielan Huang, Jinyan Xian, Qiujie Chen, Zhenjie Liu, Jianfang Feng

{"title":"Quality Assessment of Corydalis saxicola Bunting Using Quantitative Analysis of Multi-Components by Single Marker and Fingerprint Analysis","authors":"Yuanle Shen, Xu Yang, Hongli Feng, Huini Ruan, Yuan Zhou, Zihong Li, Jilang Li, Peng Zhang, Kaitong Li, Honglan Wang, Shen Liu, Xiaoxiu Shi, Jielan Huang, Jinyan Xian, Qiujie Chen, Zhenjie Liu, Jianfang Feng","doi":"10.1002/jssc.70028","DOIUrl":"10.1002/jssc.70028","url":null,"abstract":"<div>\u0000 \u0000 <p>Yan Huanglian is the whole grass and rhizome of <i>Corydalis saxicola</i> Bunting (CSB) of the Papaveraceae family. CSB has various pharmacological effects and is a promising ethnopharmaceutical. The chromatographic fingerprint of 16 batches of CSB samples was established in this study. Quantitative analysis of multi-components by single marker and the external standard method (ESM) was used to quantitatively assess seven constituents of CSB. Because of its high stability and availability, dehydrocavidine was used as a reference to determine the relative correction factors (RCFs) of columbamine, epiberberine, coptisine hydrochloride, palmatine chloride, berberine hydrochloride, and chelerythrine based on high-performance liquid chromatography. The calculated quantitative analysis of multi-components by single marker values is consistent with those obtained from the ESM, and the repeatability of the RCFs was demonstrated. For the first time, the quantitative analysis of multi-components by the single marker method has been established for the simultaneous determination of seven components, and it is demonstrated to be feasible and accurate for the quality evaluation of CSB combined with chromatographic fingerprint analysis. The combination of these methods could be used as a reference for quality control in Chinese medicine.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 22","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction of Penicillin G From Dairy Products Using Deep Eutectic Solvent/Alginate Beads Enhanced via Layered Double Hydroxides 利用层状双氢氧化物增强的深共晶溶剂/海藻酸珠提取乳制品中的青霉素 G

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-12 DOI: 10.1002/jssc.70005

Hassan Sereshti, Ali Taghavi, Sara Soltani, Hamid Rashidi Nodeh

{"title":"Extraction of Penicillin G From Dairy Products Using Deep Eutectic Solvent/Alginate Beads Enhanced via Layered Double Hydroxides","authors":"Hassan Sereshti, Ali Taghavi, Sara Soltani, Hamid Rashidi Nodeh","doi":"10.1002/jssc.70005","DOIUrl":"10.1002/jssc.70005","url":null,"abstract":"<div>\u0000 \u0000 <p>A new nanocomposite based on alginate microbeads impregnated with novel strontium–aluminum layered double hydroxide (Sr-Al LDH) incorporated with choline chloride-urea deep eutectic solvent (ChCl-U DES) was introduced. The microbeads and LDH were characterized by adsorption/desorption isotherms, Fourier-transform infrared spectroscopy, SEM, and x-ray diffraction. The adsorbent was employed for determining penicillin G (PENG) using dispersive solid-phase extraction prior to HPLC analysis. The extraction efficiency of the adsorbent, compared with that of unmodified alginate, showed a 2.5-fold increase. Significant parameters, including elution conditions, sorbent composition and mass, adsorption time, and sample pH, were optimized. The LOD was 0.4 µg kg<sup>−1</sup>, and the linear range was 1.4–500 µg kg<sup>−1</sup>. The LOQ (1.4 µg kg<sup>−1</sup>) was lower than the established maximum level for PENG by the European Union (4 µg kg<sup>−1</sup>). Enrichment factor and synthesis reproducibility were also investigated. The method's accuracy was evaluated through PENG analysis in dairy products and water, with recoveries of 81%–107% (RSDs < 7.6%). The procedure's greenness was assessed using the Analytical Eco-scale and achieved excellent green credentials. To the best of our knowledge, this is the first report on the synthesis and application of a novel alginate composite for the extraction of PENG from dairy and water samples.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 21","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Screening, Identification, and Comparison of Tripterygium Hypoglaucum Hutch Preparations by Ultra-High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Combined With Multivariate Statistical Analysis 利用超高效液相色谱-四极杆飞行时间质谱结合多元统计分析技术筛选、鉴定和比较Tripterygium Hypoglaucum Hutch制剂的化学成分。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-12 DOI: 10.1002/jssc.70023

Hui-Zhi Luo, Jie Xiang, Wan-Yu Gui, Jia-Hui Gong, Jian-Dong Zou, Chang-Yin Li

{"title":"Chemical Screening, Identification, and Comparison of Tripterygium Hypoglaucum Hutch Preparations by Ultra-High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Combined With Multivariate Statistical Analysis","authors":"Hui-Zhi Luo, Jie Xiang, Wan-Yu Gui, Jia-Hui Gong, Jian-Dong Zou, Chang-Yin Li","doi":"10.1002/jssc.70023","DOIUrl":"10.1002/jssc.70023","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Colquhounia</i> root tablets (CRT) and <i>Tripterygium hypoglaucum hutch</i> tablets (THHT), two major <i>Tripterygium hypoglaucum hutch</i> (THH) commercial preparations, have been used to treat chronic kidney diseases or rheumatic diseases. However, there have been no reports on the chemical comparison between CRT and THHT, greatly hindering the understanding of their pharmacological difference as well as their rational application in clinical practice. In the present study, ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry combined with automated data analysis by MS-DIAL software and MS-FLO website was employed to systematically screen and characterize the components in CRT and THHT. Multivariate statistical analysis was used to compare the differences between these two preparations. As a result, up to 92 components were tentatively identified, and 17 of them were characterized for the first time in THH preparations. According to the criteria of variable importance in projection (VIP) >1, <i>p</i> < 0.05, and fold change (FC) > 1.2, 46 components could be screened as major differential chemical components. Among them, phenolic acids, organic acids, amino acids, and diterpenoids were higher in CRT, while the sesquiterpene alkaloids were relatively higher in THHT. This study clarified the chemical material basis and the difference between CRT and THHT, providing a valuable reference for quality control and clinical rational use of THH preparations.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 21","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-Cysteine-Bonded Polymeric Monolithic Stationary Phase for Enantioseparation of Dansyl Amino Acids in Capillary Liquid Chromatography 毛细管液相色谱法中用于丹酰氨基酸对映体分离的 L-半胱氨酸键合聚合物整体固定相。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-10 DOI: 10.1002/jssc.70017

Ayu Rahayu Anggraeni, Lee Wah Lim, Toyohide Takeuchi

{"title":"L-Cysteine-Bonded Polymeric Monolithic Stationary Phase for Enantioseparation of Dansyl Amino Acids in Capillary Liquid Chromatography","authors":"Ayu Rahayu Anggraeni, Lee Wah Lim, Toyohide Takeuchi","doi":"10.1002/jssc.70017","DOIUrl":"10.1002/jssc.70017","url":null,"abstract":"<div>\u0000 \u0000 <p>A chiral monolith stationary phase was fabricated by modifying the monolith surface using L-cysteine through a thiol-epoxy click reaction. L-cysteine-bonded polymer monolith was characterized by scanning electron microscopy/energy-dispersive X-ray and attenuated total reflectance Fourier-transformed infrared. The monomer content and modification temperature were carefully optimized to create a polymer monolith with excellent mechanical stability and permeability. Our findings revealed that the column morphology depended significantly on the porogen concentration and modification temperature for its morphology and efficiency. Adequate pores and binding sites were formed with the optimal porogen content, while a higher modification temperature improved the modification yield, enhancing peak shapes and increasing separation efficiency. The column demonstrated its capability for enantioseparation of dansyl glutamic acid, dansyl aspartic acid, dansyl methionine, and dansyl phenylalanine using a 60 mM ammonium acetate buffer solution and acetonitrile in a 20:80 v/v ratio. It maintained good mechanical stability and repeatability with no relative standard deviation exceeding 7%. These results indicated that the L-cysteine-bonded polymer monolith has excellent potential as a chiral stationary phase.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 21","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Bionic Technology Combining Online Electrochemistry-Mass Spectrometry and Offline Electrochemistry-Liquid Chromatography-Mass Spectrometry for Simulating and Characterizing Metabolic Processes of Bioactive phenolic acids in Natural Products 结合在线电化学-质谱法和离线电化学-液相色谱-质谱法的先进仿生技术，用于模拟和表征天然产品中生物活性酚酸的代谢过程。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-08 DOI: 10.1002/jssc.70006

Kunze Du, Man Yang, Wentao Ma, Tianyu Liu, Huihui Sun, Tengteng Huang, Jin Li, Yanxu Chang

{"title":"Advanced Bionic Technology Combining Online Electrochemistry-Mass Spectrometry and Offline Electrochemistry-Liquid Chromatography-Mass Spectrometry for Simulating and Characterizing Metabolic Processes of Bioactive phenolic acids in Natural Products","authors":"Kunze Du, Man Yang, Wentao Ma, Tianyu Liu, Huihui Sun, Tengteng Huang, Jin Li, Yanxu Chang","doi":"10.1002/jssc.70006","DOIUrl":"10.1002/jssc.70006","url":null,"abstract":"<div>\u0000 \u0000 <p>The metabolism research of bioactive phenolic acids widely found in natural products is of great significance for elucidating pharmacologic mechanisms and screening lead compounds. However, it is time-consuming and vulnerable to interference to conduct the traditional metabolism approach by applying organisms or biomaterials. Herein, a bionic technology was established by combining online electrochemistry-mass spectrometry (EC-MS) with offline electrochemistry-liquid chromatography-mass spectrometry (EC-LC-MS) to investigate the oxidative transformation and metabolic processes of the active phenolic acids (including salvianolic acid A, caffeic acid, 3, 5-<i>O</i>-dicaffeoylquinic acid, ferulic acid, salvianic acid A, and protocatechuic acid). Phase I metabolism of the phenolic acids were simulated by applying a three-electrode controlled potential electrochemical reactor with a boron-doped diamond electrode, with glutathione mixed into the oxidative products simultaneously for obtaining the phase II metabolites. Finally, structural characterization of the simulated metabolites of the phenolic acids was achieved successfully, including hydroxylation, methylation, demethylation, decarboxylation, etc. It was revealed that the simulated metabolism process based on an electrochemical system was effective in yielding a wide variety of metabolites for these compounds, which was also compared with the metabolism results applying rat liver microsomes. Consequently, this bionic technology is expected to be a powerful tool to investigate the material basis for the efficacy of active ingredients of natural products.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 21","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effervescent-assisted Liquid-phase Microextraction Employing Switchable Hydrophilicity Solvent for the Determination of Cortisol and Testosterone in Oral Fluid by High-Performance Liquid Chromatography-Diode Array Detection 采用可切换亲水性溶剂的泡腾片辅助液相微萃取技术，利用高效液相色谱-二极管阵列检测法测定口服液中的皮质醇和睾酮。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-08 DOI: 10.1002/jssc.70022

Carolina Silveira Dalanhol, Camila Scheid, Josias Merib

{"title":"Effervescent-assisted Liquid-phase Microextraction Employing Switchable Hydrophilicity Solvent for the Determination of Cortisol and Testosterone in Oral Fluid by High-Performance Liquid Chromatography-Diode Array Detection","authors":"Carolina Silveira Dalanhol, Camila Scheid, Josias Merib","doi":"10.1002/jssc.70022","DOIUrl":"10.1002/jssc.70022","url":null,"abstract":"<div>\u0000 \u0000 <p>Cortisol and testosterone are important biomarkers for diagnosing complex disorders, including polycystic ovary syndrome and Cushing's syndrome, where symptomatology usually overlaps with other prevalent disorders. This work proposes, for the first time, an analytical method based on a switchable hydrophilicity solvent as an extraction phase for the determination of cortisol and testosterone in oral fluid (OF) by high-performance liquid chromatography with diode-array detection. The optimized extraction conditions consisted of 1000 µL of OF, 100 µL of decanoic acid solution (65 mg/mL), 170 µL of Na<sub>2</sub>CO<sub>3</sub>, 900 µL of H<sub>2</sub>SO<sub>4</sub> and 150 µL of acetonitrile for dilution. The method was validated, and coefficients of determination higher than 0.9926, the limit of detection of 4.55 ng/mL and the limit of quantification of 15.00 ng/mL were obtained. Intra-day precision varied from 5.6% to 11.9%, inter-day precision ranged from 6.1% to 13.5%, and relative recoveries ranged from 98.9% to 104.6% for cortisol, and 89.1% to 103.9% for testosterone. This methodology was successfully applied to five OF samples from volunteers. Moreover, the greenness of this methodology was evaluated based on the sample preparation metric of sustainability achieving a global score of 7.37 which can be considered sustainable.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 21","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Determination of Methotrexate and 7-Hydroxy-Methotrexate by UHPLC-MS3 Assay Coupled With Multiple Stage Fragmentation to Enhance Sensitivity 利用超高效液相色谱-质谱 3测定法同时测定甲氨蝶呤和 7-羟基甲氨蝶呤，并采用多级碎片化技术提高灵敏度

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-08 DOI: 10.1002/jssc.70020

Huaqiang Li, Xujian Duan, Feifei Wu, Lei Yin

{"title":"Simultaneous Determination of Methotrexate and 7-Hydroxy-Methotrexate by UHPLC-MS3 Assay Coupled With Multiple Stage Fragmentation to Enhance Sensitivity","authors":"Huaqiang Li, Xujian Duan, Feifei Wu, Lei Yin","doi":"10.1002/jssc.70020","DOIUrl":"10.1002/jssc.70020","url":null,"abstract":"<div>\u0000 \u0000 <p>A selective ultra-high performance liquid chromatography tandem mass spectrometry cubed (UHPLC/MS<sup>3</sup>) assay for simultaneous determination of methotrexate (MTX) and 7-hydroxy-methotrexate (7-OH-MTX) in human plasma was developed and validated. After protein precipitation with methanol, chromatographic separation of MTX, MTX-d<sub>3</sub>, and 7-OH-MTX was performed on a Waters AcQuity UPLC-BEH column (2.1 × 50 mm I.D., 1.7 µm) with gradient elution. MRM<sup>3</sup> transition was used for the detection of MTX (<i>m/z</i> 455.2→308.0→175.1) and 7-OH-MTX (<i>m/z</i> 471.3→191.0→148.1). The linear range of UHPLC/MS<sup>3</sup> assay for the determination of MTX and 7-OH-MTX was 0.5–300 ng/mL (<i>R</i><sup>2</sup> ≥ 0.99) and 5–1500 ng/mL (<i>R</i><sup>2</sup> ≥ 0.99), respectively. The enhanced selectivity and sensitivity are the novelties of the developed UHPLC/MS<sup>3</sup> assay. The analytical method was successfully applied to simultaneous determination of MTX and its major metabolite 7-OH-MTX in real human plasma samples.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 21","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Functionalized Pyridine Modified Magnetic Nanoparticles for Utilization in Magnetic Solid-Phase Extraction of Tebuconazole and Propiconazole Followed by Quantification via High-Performance Liquid Chromatography-Ultraviolet Detection 合成功能化吡啶修饰的磁性纳米粒子，用于戊唑醇和丙环唑的磁性固相萃取，并通过高效液相色谱-紫外检测法进行定量。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-07 DOI: 10.1002/jssc.70009

Naghmeh Nikbakhsh, Milad Ghani, Behrooz Maleki

{"title":"Synthesis of Functionalized Pyridine Modified Magnetic Nanoparticles for Utilization in Magnetic Solid-Phase Extraction of Tebuconazole and Propiconazole Followed by Quantification via High-Performance Liquid Chromatography-Ultraviolet Detection","authors":"Naghmeh Nikbakhsh, Milad Ghani, Behrooz Maleki","doi":"10.1002/jssc.70009","DOIUrl":"10.1002/jssc.70009","url":null,"abstract":"<div>\u0000 \u0000 <p>In the current study, a new magnetic sorbent (Fe<sub>3</sub>O<sub>4</sub>@2,6-diamino-4-(3,4-dihydroxyphenyl) pyridine-3,5-dicarbonitrile) was prepared as a new and efficient sorbent to be used in the magnetic solid-phase extraction method to extract tebuconazole and propiconazole as model compounds. In this regard, effective parameters such as pH of the sample solution, desorption volume, ionic strength, extraction time, desorption time, and amount of the magnetic sorbent were optimized using experimental design. In the optimal condition, the linear dynamic ranges for tebuconazole and propiconazole were between 0.5 and 200.0 µg/L. Also, the limits of detection, limits of quantification, and relative standard deviations were 0.21–0.14 µg/L, 0.49–0.45 µg/L, and 1.3%–4.6%, respectively. The estimated enrichment factors for tebuconazole and propiconazole were 22 and 28, respectively. The absolute recovery (AR%) values for tebuconazole and propiconazole were also 67% and 84%, respectively. Finally, the proposed method was used for determination of tebuconazole and propiconazole in cucumber, tomato, orange, and lemon fruits. The obtained result confirmed that the presented method is capable for the analysis of the selected analytes in complicated matrices. In addition, the ARs% were in the range of 67%–84%. The result proved that the presented method can be an alternative to the extraction process of various pesticides.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 21","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation on the Retention and Separation of Glucosinolates With a Mixed-Mode Reversed-Phase/Weak Anion-Exchange Column 使用反相/弱阴离子交换混合模式色谱柱保留和分离葡萄糖苷酸盐的研究

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-11-07 DOI: 10.1002/jssc.70007

Yanfang Li, Pei Chen, Mengliang Zhang, Jianghao Sun

{"title":"Investigation on the Retention and Separation of Glucosinolates With a Mixed-Mode Reversed-Phase/Weak Anion-Exchange Column","authors":"Yanfang Li, Pei Chen, Mengliang Zhang, Jianghao Sun","doi":"10.1002/jssc.70007","DOIUrl":"10.1002/jssc.70007","url":null,"abstract":"<div>\u0000 \u0000 <p>Glucosinolates, a crucial group of secondary metabolites in <i>Brassica</i> vegetables, present significant chromatographic separation challenges due to their anionic form, structure diversities, and co-existence of other phenolic compounds. This study comparatively investigated the retention and separation of seven glucosinolates using a mixed-mode reversed-phase/weak anion-exchange column and a conventional reversed-phase C18 column. Separation factors for each glucosinolate with its adjacent peaks were over 1.0 on the mixed-mode column, while co-eluting was observed on the C18 column. The effects of mobile phase additives and pH on the separation and retention of glucosinolates were also investigated. Results showed that glucosinolate retention was inversely related to both buffer concentration and pH. The optimized method for the mix-mode column was applied to the complex <i>Brassica</i> vegetable samples. In addition to the 17 well-resolved glucosinolate peaks, 34 peaks for phenolic compounds were identified in broccoli microgreen, suggesting the successful application scenarios for qualitative analysis in comparison with the single mode reverse phase C18 column. This study demonstrates that the mixed-mode reversed-phase/weak anion-exchange column can be used as a promising separation tool for organic anions in a complex sample matrix.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 21","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0