Journal of separation science最新文献_第10页

Microfluidics identify moso bamboo and henon bamboo by leaf protoplast subpopulations with single-cell analysis 微流体技术通过单细胞分析鉴定毛竹和楠竹的叶片原生质亚群。

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-21 DOI: 10.1002/jssc.202400120

Yameng Liu, Mingxu Wang, Xiaohu Wang

{"title":"Microfluidics identify moso bamboo and henon bamboo by leaf protoplast subpopulations with single-cell analysis","authors":"Yameng Liu, Mingxu Wang, Xiaohu Wang","doi":"10.1002/jssc.202400120","DOIUrl":"10.1002/jssc.202400120","url":null,"abstract":"Current techniques identifying herbal medicine species require marker labeling or lack systematical accuracy (expert authentication). There is an emerging interest in developing an accurate and label-free tool for herbal medicine authentication. Here, a high-resolution microfluidic-based method is developed for identifying herbal species by protoplast subpopulations. Moso bamboo and henon bamboo are used as a model to be differentiated based on protoplast. Their biophysical properties factors are characterized to be 7.09 (± 0.39) × 108 V/m2 and 6.54 (± 0.26) × 108 V/m2, respectively. Their biophysical distributions could be distinguished by the Cramér–von Mises criterion with a 94.60% confidence level. The subpopulations of each were compared with conventional flow cytometry indicating the existence of subpopulations and the differences between the two species. The subsets divided by a biophysical factor of 8.05(± 0.51) × 108 V/m2 suggest good consistency with flow cytometry. The work demonstrated the possibility of microfluidics manipulation on protoplast for medication safety use taking advantage of dielectrophoresis. The device is promising in developing a reliable and accurate way of identifying herbal species with difficulties in authentication.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid evaporative ionization mass spectrometry: A survey through 15 years of applications 快速蒸发电离质谱仪：15 年应用调查。

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-21 DOI: 10.1002/jssc.202400155

Cinzia Cafarella, Domenica Mangraviti, Francesca Rigano, Paola Dugo, Luigi Mondello

{"title":"Rapid evaporative ionization mass spectrometry: A survey through 15 years of applications","authors":"Cinzia Cafarella, Domenica Mangraviti, Francesca Rigano, Paola Dugo, Luigi Mondello","doi":"10.1002/jssc.202400155","DOIUrl":"10.1002/jssc.202400155","url":null,"abstract":"Rapid evaporative ionization mass spectrometry (REIMS) is a relatively recent MS technique explored in many application fields, demonstrating high versatility in the detection of a wide range of chemicals, from small molecules (phenols, amino acids, di- and tripeptides, organic acids, and sugars) to larger biomolecules, that is, phospholipids and triacylglycerols. Different sampling devices were used depending on the analyzed matrix (liquid or solid), resulting in distinct performances in terms of automation, reproducibility, and sensitivity. The absence of laborious and time-consuming sample preparation procedures and chromatographic separations was highlighted as a major advantage compared to chromatographic methods. REIMS was successfully used to achieve a comprehensive sample profiling according to a metabolomics untargeted analysis. Moreover, when a multitude of samples were available, the combination with chemometrics allowed rapid sample differentiation and the identification of discriminant features. The present review aims to provide a survey of literature reports based on the use of such analytical technology, highlighting its mode of operation in different application areas, ranging from clinical research, mostly focused on cancer diagnosis for the accurate identification of tumor margins, to the agri-food sector aiming at the safeguard of food quality and security.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry and network pharmacology to reveal the key effective compounds and mechanism of Shengxian decoction for ameliorating doxorubicin cardiotoxicity 超高效液相色谱-四极杆飞行时间质谱法与网络药理学相结合，揭示升仙煎对改善多柔比星心脏毒性的关键有效化合物及其作用机制

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-10 DOI: 10.1002/jssc.202300867

Guangyang Jiao, Yejian Wang, Yue Song, Yuxin Chen, Xiangcheng Fan, Qing Zhao, Tao Pang, Feng Zhang, Wansheng Chen

{"title":"Combination of ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry and network pharmacology to reveal the key effective compounds and mechanism of Shengxian decoction for ameliorating doxorubicin cardiotoxicity","authors":"Guangyang Jiao, Yejian Wang, Yue Song, Yuxin Chen, Xiangcheng Fan, Qing Zhao, Tao Pang, Feng Zhang, Wansheng Chen","doi":"10.1002/jssc.202300867","DOIUrl":"10.1002/jssc.202300867","url":null,"abstract":"Shengxian decoction, a traditional Chinese medicinal prescription, has been shown to alleviate doxorubicin-induced chronic heart failure. This study established an ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method to separate and characterize the complex chemical compositions of Shengxian decoction, and the absorbed compounds in the bio-samples of the cardiotoxicity rats with chronic heart failure after its oral delivery. Note that 116 chemical compounds were identified from Shengxian decoction in vitro, 81 more than previously detected. Based on the three-dimensional data of these compounds, 28 absorbed compounds were confirmed in vivo. Network pharmacology and molecular docking experiments indicated that timosaponin B-II, timosaponin A-III, gitogenin, and 7,8-didehydrocimigenol were recognized as the key effective compounds to exert effects against doxorubicin cardiotoxicity by acting on targets such as caspase 3, cyclin-dependent kinase 1, cyclin-dependent kinase 4, receptor tyrosine-protein kinase erbB-2, and mitogen-activated protein kinase 1 in p53 and phosphatidylinositol 3-kinase-Akt signaling pathways. This study developed the understanding of the composition of Shengxian decoction for the treatment of doxorubicin cardiotoxicity, as well as a feasible strategy to elucidate the effective constituents in traditional Chinese medicines.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The utilization of open-source microcontroller boards and single-board computers in liquid chromatography 在液相色谱中使用开源微控制器板和单板计算机。

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-10 DOI: 10.1002/jssc.202400111

Christopher Piccolo, Samuel W. Foster, Deklin Parker, Catherine Seltzer, James P. Grinias

引用次数: 0

Advancements in clinical approaches, analytical methods, and smart sampling for LC–MS-based protein determination from dried matrix spots 基于液相色谱-质谱（LC-MS）的干基质点蛋白质测定的临床方法、分析方法和智能采样的进展。

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-10 DOI: 10.1002/jssc.202400061

Léon Reubsaet, Trine Grønhaug Halvorsen

{"title":"Advancements in clinical approaches, analytical methods, and smart sampling for LC–MS-based protein determination from dried matrix spots","authors":"Léon Reubsaet, Trine Grønhaug Halvorsen","doi":"10.1002/jssc.202400061","DOIUrl":"10.1002/jssc.202400061","url":null,"abstract":"Determination of proteins from dried matrix spots using MS is an expanding research area. Mainly, the collected dried matrix sample is whole blood from a finger or heal prick, resulting in dried blood spots. However as other matrices such as plasma, serum, urine, and tear fluid also can be collected in this way, the term dried matrix spot is used as an overarching term. In this review, the focus is on advancements in the field made from 2017 up to 2023. In the first part reviews concerning the subject are discussed. After this, advancements made for clinical purposes are highlighted. Both targeted protein analyses, with and without the use of affinity extractions, as well as untargeted, global proteomic approaches are discussed.In the last part, both methodological advancements are being reviewed as well as the possibility to integrate sample preparation steps during the sample handling. The focus, of this so-called smart sampling, is on the incorporation of cell separation, proteolysis, and antibody-based affinity capture.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of deep eutectic solvents on extraction of flavonoids 应用深共晶溶剂萃取黄酮类化合物。

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-10 DOI: 10.1002/jssc.202300925

Ying Hao, Fengxia Pei, Jingjing Huang, Guizhen Li, Chenglin Zhong

引用次数: 0

Simultaneous determination of six catechins and caffeine in tea and wine using salting-out assisted liquid–liquid extraction and high-performance liquid chromatography with ultraviolet detection 采用盐析辅助液液萃取和高效液相色谱-紫外检测法同时测定茶叶和葡萄酒中的六种儿茶素和咖啡因。

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-10 DOI: 10.1002/jssc.202400142

Jorge A. Custodio-Mendoza, Patryk Pokorski, Havva Aktaş, Antonia M. Carro, Marcin A. Kurek

{"title":"Simultaneous determination of six catechins and caffeine in tea and wine using salting-out assisted liquid–liquid extraction and high-performance liquid chromatography with ultraviolet detection","authors":"Jorge A. Custodio-Mendoza, Patryk Pokorski, Havva Aktaş, Antonia M. Carro, Marcin A. Kurek","doi":"10.1002/jssc.202400142","DOIUrl":"10.1002/jssc.202400142","url":null,"abstract":"Catechins, renowned for their antioxidant properties and health benefits, are commonly present in beverages, particularly tea and wine. An efficient and cost-effective salting-out assisted liquid–liquid extraction (SALLE) method has been developed and validated for the simultaneous determination of six catechins and caffeine in tea and wine samples using high-performance liquid chromatography–ultraviolet (HPLC–UV). This method demonstrates outstanding performance: linearity (1–120 µg/mL, r2 > 0.999), accuracy (96.5%–103.4% recovery), and precision (≤14.7% relative standard deviation), meeting validation requirements set by the US Food and Drug Administration. The reduced sample size (0.1 g) minimizes matrix interferences and costs without compromising sensitivity. All analytes were detected in Camellia sinensis teas, with green tea displaying the highest total catechin content (47.5–100.1 mg/mL), followed by white and black teas. Analysis of wine samples reveals the presence of catechin in all red and white wines, and epigallocatechin gallate in all red wine samples, highlighting the impact of winemaking processes on catechin content. The SALLE–HPLC–UV approach represents a green alternative by eliminating organic waste, surpassing conventional dilution methods in specificity and sensitivity for catechin determination. AGREEprep assessment emphasizes the strengths of the SALLE procedure, including material reusability, throughput efficiency, minimal sample requirements, low energy consumption, and the absence of organic waste generation.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retention mechanisms of amitriptyline and its impurities on amide, amino, diol, and silica columns in hydrophilic interaction liquid chromatography 亲水作用液相色谱法中阿米替林及其杂质在酰胺柱、氨基柱、二元醇柱和硅胶柱上的保留机理。

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-10 DOI: 10.1002/jssc.202300949

Darija Knežević Ratković, Radovan Kukobat, Irena Kasagić-Vujanović

{"title":"Retention mechanisms of amitriptyline and its impurities on amide, amino, diol, and silica columns in hydrophilic interaction liquid chromatography","authors":"Darija Knežević Ratković, Radovan Kukobat, Irena Kasagić-Vujanović","doi":"10.1002/jssc.202300949","DOIUrl":"10.1002/jssc.202300949","url":null,"abstract":"Hydrophilic interaction liquid chromatography (HILIC) has been widely applied to challenging analysis in biomedical and pharmaceutical fields, bridging the gap between normal-phase high-performance liquid chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC). This paper comprehensively explores the retention mechanisms of amitriptyline and its impurities A, B, C, D, F, and G on amide, amino, diol, and silica columns. Dual HILIC/RP-HPLC retention mechanisms were developed, and transitional points between HILIC and RP-HPLC mechanisms were calculated on amide, diol, and silica columns. Adsorption and partition contributions to overall retention mechanisms were evaluated using Python software in HILIC and RP-HPLC regions. The cation exchange mechanism dominates overall retention for ionized analytes in the silica column (R2 > 0.995), whereas the retention of ionized analytes increases with pH. Impacts of acetonitrile content, buffer ionic strength, and pH, along with their interactions on the retention of ionized analytes in the silica column, were determined using the chemometric approach. Acetonitrile content showed the most significant impact on the retention mechanisms. These findings highlight that a detailed investigation into retention mechanisms provides notable insights into factors influencing analyte retention and separation, promising valuable guidance for future analysis.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and separation of natural anticancer active ingredients related to phospholipase C 筛选和分离与磷脂酶 C 有关的天然抗癌活性成分。

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-10 DOI: 10.1002/jssc.202300898

Na Liu, Zili Yue, Shuang Hu, Rongrong Xing, Runqin Wang, Li Yang, Xuan Chen

{"title":"Screening and separation of natural anticancer active ingredients related to phospholipase C","authors":"Na Liu, Zili Yue, Shuang Hu, Rongrong Xing, Runqin Wang, Li Yang, Xuan Chen","doi":"10.1002/jssc.202300898","DOIUrl":"10.1002/jssc.202300898","url":null,"abstract":"Based on the specific binding of drug molecules to cell membrane receptors, a screening and separation method for active compounds of natural products was established by combining phospholipase C (PLC) sensitized hollow fiber microscreening by a solvent seal with high-performance liquid chromatography technology. In the process, the factors affecting the screening were optimized. Under the optimal screening conditions, we screened honokiol (HK), magnolol (MG), negative control drug carbamazepine, and positive control drug amentoflavone, the repeatability of the method was tested. The PLC activity was determined before and after the screening. Experimental results showed that the sensitization factors of PLC of HK and MG were 61.0 and 48.5, respectively, and amentoflavone was 15.0, carbamazepine could not bind to PLC. Moreover, the molecular docking results were consistent with this measurement, indicating that HK and MG could be combined with PLC, and they were potential interacting components with PLC. This method used organic solvent to seal the PLC greatly ensuring the activity, so this method had the advantage of integrating separation, and purification with screening, it not only exhibited good reproducibility and high sensitivity but was also suitable for screening the active components in natural products by various targets in vitro.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research of saccharides and related biocomplexes: A review with recent techniques and applications 糖和相关生物复合物的研究：最新技术和应用综述

IF 3.1 3区工程技术

Journal of separation science Pub Date : 2024-05-03 DOI: 10.1002/jssc.202300668

Heli Sirén

引用次数: 0