Journal of separation science最新文献

{"title":"Comprehensive Chemical Profiling and Quantitative Determination of Solanum americanum Mill. and S. nigrum Linn. Combined by UHPLC-HRMS With Feature-Based Molecular Networking","authors":"Lingyi Xin,&nbsp;Xuemei Wei,&nbsp;Renyan Liu,&nbsp;Yun Li,&nbsp;Yang Yang,&nbsp;Yongtong Huang,&nbsp;Yu Zhang,&nbsp;Baodong Feng,&nbsp;Linqi Su,&nbsp;Quanxi Mei,&nbsp;Qinhua Chen,&nbsp;Guangyi Yang","doi":"10.1002/jssc.70469","DOIUrl":"10.1002/jssc.70469","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Solanum americanum</i> Mill. and <i>S. nigrum</i> Linn. are traditional Solanaceae-derived herbal medicines. Their sprouts and mature fruits are utilized as both vegetables and medicinal herbs. Although <i>S. americanum</i> Mill. is classified as a primary variety of <i>S. nigrum</i> Linn., significant pharmacological variations and incompletely characterized phytochemical profiles differentiate their applications. In this study, UHPLC/LTQ-Orbitrap mass spectrometry (HRMS) (Thermo Fisher Scientific, USA), assisted with feature-based molecular networking, was applied for in-depth profiling of <i>S. americanum</i> Mill. and <i>S. nigrum</i> Linn. With the help of feature-based molecular networking, five clusters were characterized, including flavonoids, phenylpropanoids, fatty acids, alkaloids, and steroids. <i>S. americanum</i> Mill. demonstrated markedly higher relative abundance in flavanols than <i>S. nigrum</i> Linn., potentially elucidating its distinct antioxidant activity and its anti-inflammatory and antibacterial efficacy. In addition, metabolomics coupled with multivariate analyses screened and identified 16 discriminative chemical markers enabling differentiation between <i>S. americanum</i> Mill. and <i>S. nigrum</i> Linn. Building on the above, to elucidate the chemical basis underlying their distinct pharmacological activities, we quantified 13 identified key differential constituents of <i>S. americanum</i> Mill. and <i>S. nigrum</i> Linn. extracts by UHPLC-QTRAP-MS/MS using the multiple reaction monitoring (MRM) mode. The average contents of isoquercitrin, rutin, protocatechuic acid, and chlorogenic acid in <i>S. americanum</i> Mill. are significantly higher than those in <i>S. nigrum</i> Linn., suggesting that these components may serve as potential quality markers for <i>S. americanum</i> Mill. This study elucidates the chemical basis underlying <i>S. americanum</i> Mill. and <i>S. nigrum</i> Linn.’s pharmacological activity differences, establishes quality control tools, and supports <i>S. americanum</i> Mill.’s rational utilization in herbal medicine development.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Separation and Quantification of Tetracycline Antibiotics Using Ionic Liquid-Functionalized MOF-Derived Diffusive Gradients in Thin Films","authors":"Hanbing Li,&nbsp;Jie Wang,&nbsp;Yuyang Li,&nbsp;Yaming Li,&nbsp;Lei Yan,&nbsp;Sumei Li,&nbsp;Dayi Zhang,&nbsp;Yixuan Liang,&nbsp;Danyang Li,&nbsp;Xiaowei Wang,&nbsp;Sha Chen","doi":"10.1002/jssc.70470","DOIUrl":"10.1002/jssc.70470","url":null,"abstract":"<div>\u0000 \u0000 <p>A selective phase for tetracycline antibiotics (TAs) uptake was designed by incorporating [BMIM][PF₆]-functionalized ZIF-67-derived carbonaceous material into the diffusive gradients in a thin-films (DGT) device. This material, integrated in the binding gel of DGT (ta-DGT), exhibited specifically strong affinity toward tetracycline (TC), oxytetracycline (OTC), and chlortetracycline (CTC), with adsorption capacities of 12.22, 11.81, and 13.78 µg/disc, respectively. The ta-DGT showed preferential uptake of TAs over coexisting antibiotics and organic contaminants, with adsorption ratios above 80% and stable performance over pH 4–9 and ionic strength of 0.001–0.1 M. Mechanistic analysis indicated that π–π electron donor–acceptor interactions and cation–π bonding facilitated the specific targeting of TAs onto ta-DGT. During 7 days of field deployment in a domestic wastewater treatment plant in Beijing, ta-DGT showed 5–40 times higher detectable concentrations than conventional HLB/XAD DGT devices. These results validated its sensitivity, reliability, and feasibility for the selective separation and quantification of TAs in complex aqueous matrices.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modern Optimization Strategies in High-Performance Liquid Chromatography Analysis","authors":"Samar H. Elagamy,&nbsp;Rehab H. Elattar","doi":"10.1002/jssc.70487","DOIUrl":"10.1002/jssc.70487","url":null,"abstract":"<div>\u0000 \u0000 <p>Optimization is a critical step in chromatographic method development. High-performance liquid chromatography (HPLC) remains the method of choice for pharmaceutical and biological analysis; however, traditional method development has relied on trial-and-error strategies. These approaches are often laborious, time-consuming, and highly dependent on analyst experience. To address these limitations, structured and data-driven optimization strategies such as Design of Experiments and Analytical Quality by Design have been introduced to improve efficiency and reproducibility. Nevertheless, these approaches often assume ideal system behavior and may not fully capture real-world chromatographic complexity, including peak tailing, matrix interference, and atypical biomolecule retention, particularly in advanced HPLC modes. In this context, artificial intelligence (AI) has emerged as a powerful tool to address nonlinear, multidimensional chromatographic systems. This review provides a comprehensive overview of classical, statistical, and AI-driven optimization strategies in HPLC, highlighting their advantages, limitations, and practical applications. It also proposes a structured framework for selecting appropriate optimization approaches based on system complexity and data availability.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Pyrrolizidine Alkaloids and Their N‑Oxides in Bee Pollen by Accelerated Solvent Extraction Followed by Magnetic Covalent Organic Framework-Based Offline Purification and Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry","authors":"Yuhang Wang,&nbsp;Yujia Guo,&nbsp;Guoda Wang,&nbsp;Xiao Feng,&nbsp;Mingrong Qian,&nbsp;Zuguang Li","doi":"10.1002/jssc.70488","DOIUrl":"10.1002/jssc.70488","url":null,"abstract":"<div>\u0000 \u0000 <p>Pyrrolizidine alkaloids (PAs) and their N-oxides (PANOs) are naturally occurring toxic plant metabolites that have attracted widespread attention due to their potential food safety hazards. In this study, an efficient analytical method for determining PAs/PANOs in bee pollen was established by combining accelerated solvent extraction (ASE) with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A magnetic covalent organic framework material (Fe₃O₄@COF-TAPB-TFP) was synthesized and used as an adsorbent for offline purification to reduce matrix interference and improve detection sensitivity. Under optimal conditions, the method showed excellent linearity (<i>R</i>² ≥ 0.99) in the range of 1–200 µg·L⁻¹, with recoveries of 70.1%–95.6% for spiked blank samples at three levels (10, 50, and 100 µg·kg⁻¹), relative standard deviations below 15%, and limits of quantification of 1.2–9.9 µg·kg⁻¹. This method was successfully applied to the analysis of local market bee pollen samples, with detected PAs/PANOs levels ranging from 25.6 to 178.6 µg·kg⁻¹. Overall, the Fe₃O₄@COF-TAPB-TP-assisted ASE-UHPLC-MS/MS method provides an accurate, rapid, highly automated, and high-throughput platform for monitoring PAs/PANOs contamination in food products.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of Co-Cyromazine Complexes Onto Halloysite Nanotubes as a Spin-Tip Solid-Phase Extraction Sorbent for Determining Hydrophilic B Vitamins in Functional Foods","authors":"Jiaheng Yan,&nbsp;Yifan Xu,&nbsp;Yihui Chen,&nbsp;Zhicheng Bai,&nbsp;Jianfeng He,&nbsp;Meikang Lei,&nbsp;Xiaoqiang Qiao,&nbsp;Tingting Wang","doi":"10.1002/jssc.70489","DOIUrl":"10.1002/jssc.70489","url":null,"abstract":"<div>\u0000 \u0000 <p>Quantifying hydrophilic B vitamins (B1 [(thiamine], B2 [riboflavin], and B3 [nicotinic acid]) in complex functional food matrices presents a significant challenge. Conventional sorbents, such as C18, often exhibit poor retention and low recoveries (50%–60%) due to weak hydrophilic interactions. To efficiently extract these target analytes from functional foods, a new sorbent with tailored hydrophilic affinity has been developed by incorporating Co-cyromazine complexes onto halloysite nanotubes. This composite was employed as the sorbent for a spin-tip solid-phase extraction method, which was coupled with liquid chromatography-tandem mass spectrometry analysis. Following comprehensive optimization and validation, the method demonstrates simplicity, accuracy, sensitivity, and cost-effectiveness. It requires only 10 mg (or 10 µL) of functional food sample, while delivering high recoveries (81.3%–107.8%) with good precision (relative standard deviations ≤10.7%). The limits of detection ranged from 0.4 to 2.5 ng/mL in liquid matrices and from 0.7 to 8.7 ng/g in solid matrices. Practical applicability was confirmed through the successful quantification of vitamins B1, B2, and B3 in various commercial functional foods. Consequently, the developed method provides a reliable and promising analytical tool for the determination of B vitamins, with the potential for extension to other hydrophilic analytes in biological and food matrices.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Overview of Pterin Analysis in Biological Samples: From Occurrence and Properties to Sample Pretreatment Combined With Hyphenated Separation Techniques","authors":"Jindřich Brejcha,&nbsp;Zuzana Bosakova","doi":"10.1002/jssc.70483","DOIUrl":"10.1002/jssc.70483","url":null,"abstract":"<p>Pterins are a structurally diverse group of biologically active compounds within the pteridine family, with key roles in pigmentation, redox metabolism, light sensing, and cellular signaling across a wide range of organisms. Their quantification in biological samples is analytically demanding due to their high polarity, chemical instability, and the presence of multiple oxidation states. This review presents an integrated overview of pterin occurrence, structure, and physicochemical properties, followed by a detailed discussion of sample preparation strategies designed to ensure compound stability and analytical accuracy. Methods such as chemical oxidation, photochemical derivatization, and antioxidant stabilization are evaluated in the context of various biological matrices. We further examine state-of-the-art analytical techniques that combine separation with detection, including capillary electrophoresis, gas and liquid chromatography coupled with fluorescence, UV, electrochemical, or mass spectrometric detection. Particular attention is given to recent advances in LC–MS techniques, including both tandem mass spectrometry (LC–MS/MS) and high-resolution approaches (e.g., HPLC–Q/TOF-MS), which have greatly improved the sensitivity, selectivity, and throughput of pterin analysis, especially in combination with HILIC separation mode. These developments support the growing use of pterins as biomarkers in clinical diagnostics and physiological research, and underscore the importance of robust, matrix-appropriate analytical protocols tailored to the specific challenges posed by this compound class.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70483","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A High-Throughput Analytical Approach Using Polyaniline Doped With Oxalic Acid in Thin Film Solid-Phase Microextraction for the Determination of Personal Care Products in Recreational Waters","authors":"Amanda Vitória Santos,&nbsp;Francielle Crocetta Turazzi,&nbsp;Eduardo Carasek,&nbsp;Guilherme Mariz de Oliveira Barra","doi":"10.1002/jssc.70478","DOIUrl":"10.1002/jssc.70478","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, a high-throughput analytical methodology was developed based on the thin film solid-phase microextraction (TF-SPME) technique, employing polyaniline (PAni) as the extractor phase for the determination of personal care products in recreational water samples. The compounds investigated included three parabens, methylparaben (MP), ethylparaben (EP), butylparaben (BP), the UV filter and benzophenone (BZP), with detection performed by high-performance liquid chromatography with a diode-array detector (HPLC-DAD). Experimental conditions were optimized using univariate and multivariate approaches, evaluating the effects of the extractor phase, desorption solvent and time, sample pH, extraction time, and NaCl concentration. Optimal extraction conditions involved the use of PAni doped with oxalic acid, desorption in a mixture of ACN:MeOH (50:50) for 30 min, an extraction time of 150 min, sample pH adjusted to 7, and 20% NaCl. Calibration curves were performed in ultrapure water with determination coefficients greater than 0.9973. The limits of detection (LODs) and quantification (LOQs) were 1.5 and 5 µg L⁻¹, respectively, for all analytes. Intraday precision ranged from 2% to 19%, while inter-day precision varied between 10% and 22%. Method accuracy was assessed through relative recovery experiments in a water sample from a swimming pool, with recoveries ranging from 74% to 109%. The developed method was applied to six recreational water samples. In three of them, the analyte concentrations were above the LOQ, while in the others, the analytes were not found or their concentrations were below the LOD.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148368770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatographic Purification of Complex Natural Products as a Decision Problem: Retention Prediction, Adaptive Optimization, and Experimental Feedback","authors":"Yifei Zhao,&nbsp;Yuxuan Han,&nbsp;Anni Zhou,&nbsp;Xiaoming Peng,&nbsp;Simin Yang,&nbsp;Pinpin Feng","doi":"10.1002/jssc.70484","DOIUrl":"10.1002/jssc.70484","url":null,"abstract":"<div>\u0000 \u0000 <p>Chromatographic purification of complex natural products is not only a problem of improving analytical resolution, but also a sequence of decisions involving uncertain targets, variable matrices, limited samples, competing objectives, and scale-dependent constraints. Data-assisted chromatography can support this process, but current evidence does not yet establish the autonomous purification of crude natural-product extracts. This review reframes the field as decision support for staged purification workflows, including analytical profiling, target localization, fraction enrichment, semi-preparative transfer, preparative isolation, and purity or recovery confirmation. We evaluate how retention prediction, global retention modeling, Bayesian optimization, active learning, reinforcement learning, and feedback-driven method development can support different decisions, and where their current boundaries lie. Particular attention is given to chromatographic decision quality, defined by improvements in relevant outcomes such as elution-window selection, target enrichment, fraction purity, recovery, activity retention, solvent burden, robustness, and preparative transferability. The review distinguishes currently implementable tools, near-term human-supervised workflows, and aspirational autonomous systems. Worked examples and evidence-readiness tables are used to show how prediction, optimization, feedback, and expert review can be connected without overstating the maturity of the field.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Covalent Zwitterionic Peptide-Based Antifouling Coating of the Fused Silica Capillary Applied for CE Separation of Proteins","authors":"Qinghua Cao,&nbsp;Shuai Lv,&nbsp;Yue Wang,&nbsp;Xijie Wang,&nbsp;Hailin Cong,&nbsp;Bing Yu","doi":"10.1002/jssc.70479","DOIUrl":"10.1002/jssc.70479","url":null,"abstract":"<div>\u0000 \u0000 <p>Zwitterionic compounds exhibit strong hydrophilicity, act as hydrogen bond receptors and donors, and form robust hydration layers through ionic solvation, which effectively prevent unwanted protein adsorption. To reduce nonspecific interactions between analytes and the inner surface of the separation compartment and then improve detection sensitivity and separation efficiency, it is essential to modify fused silica capillaries with functional groups. In this study, we synthesized a zwitterionic antifouling peptide (Glu-Lys)₃Glu using solid-phase peptide synthesis. Then, we bound the peptide to the inner surface of a fused silica capillary using diazo resin as a coupling agent, resulting in stable and excellent antifouling and separation abilities. To evaluate the properties of the coating, we conducted fluorescent protein adsorption and electrophoresis experiments. The obtained results demonstrated that the coating effectively prevented protein adsorption and made it possible to separate five standard proteins in 20 min. Moreover, three proteins of egg white were separated within 15 min. These findings confirmed the very good anti-protein adsorption performance of the peptide-coated capillary and its practical value for various applications.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148368789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Determination of Dechlorane-Related Compounds in Fish Muscle by Microwave-Assisted Extraction Combined With Enhanced Matrix Removal for Lipids Cleanup and Gas Chromatography–Tandem Mass Spectrometry","authors":"Shuai Zhu,&nbsp;Ka Huang,&nbsp;Wei Pang","doi":"10.1002/jssc.70480","DOIUrl":"10.1002/jssc.70480","url":null,"abstract":"<div>\u0000 \u0000 <p>A robust and efficient analytical method was developed for the simultaneous determination of eight dechlorane-related compounds in fish muscle using microwave-assisted extraction combined with enhanced matrix removal for lipids cleanup and gas chromatography–tandem mass spectrometry. The method enables effective extraction of hydrophobic analytes from lipid-rich matrices and efficient removal of co-extracted lipids, resulting in improved analytical performance in terms of recovery and reproducibility. Under optimized conditions, the method exhibited good linearity (<i>R</i>² = 0.9976–0.9997), low detection limits (method detection limits: 0.021–0.078 ng/g; limits of quantification: 0.067–0.248 ng/g), and satisfactory recoveries (77.6%–90.1%) with satisfactory precision (relative standard deviations: 2.9%–8.5%). Matrix effects were effectively controlled using matrix-matched calibration combined with isotope-labeled internal standards. The method was successfully applied to real fish samples, demonstrating reliable quantification of trace-level dechloranes. Owing to its simplified workflow and improved throughput, the proposed method provides a practical and reliable approach for the routine analysis of dechloranes in high-lipid biological matrices.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 7","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148368825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}