Journal of separation science最新文献_第9页

Screening and isolation of quorum sensing inhibitors of Pseudomonas aeruginosa from Phellodendron amurense extracts using bio-affinity chromatography 利用生物亲和层析技术从黄柏提取物中筛选和分离铜绿假单胞菌的法定量感应抑制剂。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-02 DOI: 10.1002/jssc.202400222

Yu Yi, Ye Zhou, Susu Lin, Kefan Shi, Jianfeng Mei, Guoqing Ying, Shujiang Wu

{"title":"Screening and isolation of quorum sensing inhibitors of Pseudomonas aeruginosa from Phellodendron amurense extracts using bio-affinity chromatography","authors":"Yu Yi, Ye Zhou, Susu Lin, Kefan Shi, Jianfeng Mei, Guoqing Ying, Shujiang Wu","doi":"10.1002/jssc.202400222","DOIUrl":"10.1002/jssc.202400222","url":null,"abstract":"Drug-resistant bacterial infections pose a significant challenge in the field of bacterial disease treatment. Finding new antibacterial pathways and targets to combat drug-resistant bacteria is crucial. The bacterial quorum sensing (QS) system regulates the expression of bacterial virulence factors. Inhibiting bacterial QS and reducing bacterial virulence can achieve antibacterial therapeutic effects, making QS inhibition an effective strategy to control bacterial pathogenicity. This article mainly focused on the PqsA protein in the QS system of Pseudomonas aeruginosa. An affinity chromatography medium was developed using the SpyTag/SpyCatcher heteropeptide bond system. Berberine, which can interact with the PqsA target, was screened from Phellodendron amurense by affinity chromatography. We characterized its structure, verified its inhibitory activity on P. aeruginosa, and preliminarily analyzed its mechanism using molecular docking technology. This method can also be widely applied to the immobilization of various protein targets and the effective screening of active substances.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 15","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-assisted matrix solid-phase extraction based on deep eutectic solvents and zinc oxide: Extraction and determination of six active ingredients in Ligustri Lucidi Fructus 基于深共晶溶剂和氧化锌的超声辅助基质固相萃取：萃取并测定Ligustri Lucidi Fructus中的六种活性成分。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-02 DOI: 10.1002/jssc.202400275

Chengjialu Qian, Shizhao Wang, Hongyan Chen, Jiankuan Li

{"title":"Ultrasound-assisted matrix solid-phase extraction based on deep eutectic solvents and zinc oxide: Extraction and determination of six active ingredients in Ligustri Lucidi Fructus","authors":"Chengjialu Qian, Shizhao Wang, Hongyan Chen, Jiankuan Li","doi":"10.1002/jssc.202400275","DOIUrl":"10.1002/jssc.202400275","url":null,"abstract":"In this study, we propose a novel strategy utilizing deep eutectic solvents (DESs) as both the extraction solvent and dispersing liquid, with nanometer zinc oxide (ZnO) serving as the adsorbent. This method incorporates ultrasound-assisted matrix solid phase dispersion (UA-MSPD) for the extraction of six active components (salidroside, echinacoside, acteoside, specnuezhenide, nuezhenoside G13, and oleanolic acid) from Ligustri Lucidi Fructus samples. The extracts were then analyzed using high-performance liquid chromatography equipped with a diode array detector. The effects of various parameters such as dispersant dosage, DESs volume, grinding time, ultrasonication duration, and eluent volume on extraction recovery were investigated and optimized using a central composite design under response surface methodology. The optimized conditions yielded detection limits ranging from 0.003 to 0.01 mg/g and relative standard deviations of 8.7% or lower. Extraction recoveries varied between 93% and 98%. The method demonstrated excellent linearity for the analytes (R2 ≥ 0.9997). The simple, green, and efficient DESs/ZnO-UA-MSPD technique proved to be rapid, accurate, and reliable for extracting and analyzing the six active ingredients in Ligustri Lucidi Fructus samples.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 15","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ultra-fast ultra-high-performance liquid chromatography-tandem mass spectrometry method for estimating the in vitro metabolic stability of palbociclib in human liver microsomes: In silico study for metabolic lability, absorption, distribution, metabolism, and excretion features, and DEREK alerts screening 超快速超高效液相色谱-串联质谱法估算人肝微粒体中帕博西来（palbociclib）的体外代谢稳定性：代谢稳定性、吸收、分布、代谢和排泄特征的硅学研究以及 DEREK 警报筛选。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-01 DOI: 10.1002/jssc.202400346

Mohamed W. Attwa, Ali S. Abdelhameed, Adnan A. Kadi

{"title":"An ultra-fast ultra-high-performance liquid chromatography-tandem mass spectrometry method for estimating the in vitro metabolic stability of palbociclib in human liver microsomes: In silico study for metabolic lability, absorption, distribution, metabolism, and excretion features, and DEREK alerts screening","authors":"Mohamed W. Attwa, Ali S. Abdelhameed, Adnan A. Kadi","doi":"10.1002/jssc.202400346","DOIUrl":"10.1002/jssc.202400346","url":null,"abstract":"Palbociclib (Ibrance; Pfizer) was approved for the management of metastatic breast cancer characterized by hormone receptor-positive/human epidermal growth factor receptor 2 negative status. The objective of this study was to create a fast, precise, environmentally friendly, and highly sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry approach for quantifying palbociclib (PAB) in human liver microsomes with the application for assessing metabolic stability. The validation features were performed in agreement with the bioanalytical method validation standards outlined by the US Food and Drug Administration. The StarDrop software (WhichP450 and DEREK modules) was used in screening the metabolic lability and structural alerts of PAB. The separation of PAB and encorafenib (as an internal standard) was achieved on a C8 column, employing an isocratic mobile phase. The inter-day and intra-day accuracy and precision ranged from -6.00% to 4.64% and from -2.33% to 3.13%, respectively. The constructed calibration curve displayed a linearity in the range of 1–3000 ng/mL. The sensitivity of the established approach was proven by the lower limit of quantification of 0.73 ng/mL. The Analytical GREEness calculator results revealed the high level of greenness of the developed method. The PAB's metabolic stability (t1/2 of 18.5 min and a moderate clearance (Clint) of 44.8 mL/min/kg) suggests a high extraction ratio medication that matched the WhichP450 software results.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 15","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ultra-fast green ultra-high-performance liquid chromatography-tandem mass spectrometry method for estimating the in vitro metabolic stability of zotizalkib in human liver microsomes 超快速绿色超高效液相色谱-串联质谱法估算人肝微粒体中佐替扎克布的体外代谢稳定性。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-01 DOI: 10.1002/jssc.202400393

Mohamed W. Attwa, Ali S. Abdelhameed, Adnan A. Kadi

{"title":"An ultra-fast green ultra-high-performance liquid chromatography-tandem mass spectrometry method for estimating the in vitro metabolic stability of zotizalkib in human liver microsomes","authors":"Mohamed W. Attwa, Ali S. Abdelhameed, Adnan A. Kadi","doi":"10.1002/jssc.202400393","DOIUrl":"10.1002/jssc.202400393","url":null,"abstract":"Zotizalkib (ZTK, TPX-0131) is a fourth-generation highly effective inhibitor of wild-type anaplastic lymphoma kinase (ALK) and ALK-resistant mutations that can penetrate the central nervous system. It exhibited greater potency compared to all five officially approved ALK inhibitors. The aim of this study was to develop a rapid, accurate, eco-friendly, and highly sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for measuring the concentration of ZTK in human liver microsomes (HLMs). The validation aspects of the current UHPLC-MS/MS methodology in the HLMs were conducted in accordance with the bioanalytical method validation standards specified by the US Food and Drug Administration. ZTK and encorafenib were separated using an Agilent C8 column (Eclipse Plus) and an isocratic mobile phase. The calibration curve for the developed ZTK exhibited a linear relationship within the concentration range of 1–3000 ng/mL. The results from the Analytical Green-ness Metric Approach program (0.76) suggested that the created method demonstrated a significant degree of environmental sustainability. The in vitro half-life (t1/2) and intrinsic clearance (Clint) of ZTK were determined to be 15.79 min and 51.35 mL/min/kg, respectively that suggests the ZTK exhibits characteristics similar to those of a medication with a high extraction ratio. These approaches are crucial for the progress of novel pharmaceutical development, especially in improving metabolic stability.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 15","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic materials as adsorbents for the pre-concentration and separation of active ingredients from herbal medicine 磁性材料作为吸附剂用于中药活性成分的预浓缩和分离。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-29 DOI: 10.1002/jssc.202400274

Hezhao Bai, Guohua Teng, Chen Zhang, Jingyi Yang, Wenzhi Yang, Fei Tian

{"title":"Magnetic materials as adsorbents for the pre-concentration and separation of active ingredients from herbal medicine","authors":"Hezhao Bai, Guohua Teng, Chen Zhang, Jingyi Yang, Wenzhi Yang, Fei Tian","doi":"10.1002/jssc.202400274","DOIUrl":"10.1002/jssc.202400274","url":null,"abstract":"Herbal medicine (HM) is crucial in disease management and contains complex compounds with few active pharmacological ingredients, presenting challenges in quality control of raw materials and formulations. Effective separation, identification, and analysis of active components are vital for HM efficacy. Traditional methods like liquid-liquid extraction and solid-phase extraction are time-consuming and environmentally concerning, with limitations such as sorbent issues, pressure, and clogging. Magnetic solid-phase extraction uses magnetic sorbents for targeted analyte separation and enrichment, offering rapid, pressure-free separation. However, inorganic magnetic particles’ aggregation and oxidation, as well as lack of selectivity, have led to the use of various coatings and modifications to enhance specificity and selectivity for complex herbal samples. This review delves into magnetic composites in HM pretreatment, specifically focusing on encapsulated or modified magnetic nanoparticles and materials like silica, ionic liquids, graphene family derivatives, carbon nanotubes, metal-organic frameworks, covalent organic frameworks, and molecularly imprinted polymers.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 14","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of stability indicating assay method for mitapivat: Identification of novel hydrolytic, photolytic, and oxidative forced degradation products employing quadrupole-time of flight mass spectrometry 米他匹伐稳定性指示检测方法的开发与验证利用四极杆飞行时间质谱法鉴定新型水解、光解和氧化强迫降解产物。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-25 DOI: 10.1002/jssc.202400173

Manasi Ashok Bagul, Yatesh Patil, Sayalee Sanjay Mane, Anandhu Kunnath Shaji, Pintu Das, Om Prakash Ranjan, Swapnil Jayant Dengale

{"title":"Development and validation of stability indicating assay method for mitapivat: Identification of novel hydrolytic, photolytic, and oxidative forced degradation products employing quadrupole-time of flight mass spectrometry","authors":"Manasi Ashok Bagul, Yatesh Patil, Sayalee Sanjay Mane, Anandhu Kunnath Shaji, Pintu Das, Om Prakash Ranjan, Swapnil Jayant Dengale","doi":"10.1002/jssc.202400173","DOIUrl":"10.1002/jssc.202400173","url":null,"abstract":"Mitapivat is a novel, first-in-class orally active pyruvate kinase activator approved by the US Food and Drug Administration in 2022 for the treatment of hemolytic anemia. There is no literature available regarding the identification of degradation impurities of mitapivat. The present study deals with the degradation behavior of mitapivat under various stress conditions such as hydrolytic, photolytic, thermal, and oxidative stress. The multivariate analysis found that the independent variables, that is, molarity, temperature, and time, are interacting with each other to affect the degradation of mitapivat. A specific, accurate, and precise high-performance liquid chromatographic (HPLC) method was developed to separate mitapivat from its degradation products. The separation was achieved on the C-18 column (250 mm × 4.6 mm × 5 µm) using the combination of 0.1% formic acid buffer and acetonitrile in gradient elution profile. The method was validated as per the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use Q2(R2) guideline. LC-electrospray ionization-Quadrupole-time of flight was employed to identify degradation products. A total of seven novel degradation products of mitapivat were identified based on tandem mass spectrometry and accurate mass measurement. In-silico toxicity of mitapivat and its degradation products was qualitatively evaluated by the DEREK toxicity prediction tool.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 14","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrophilic interaction chromatographic evaluation of zwitterionic polymer grafted silica gel via multiple binding sites 通过多结合位点对接枝硅胶的亲水相互作用色谱进行评估。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-25 DOI: 10.1002/jssc.202400065

Pengcheng Zhang, Yongxing Hu, Kunling Liu, Yaming Sun, Lijun He, Wenjie Zhao

{"title":"Hydrophilic interaction chromatographic evaluation of zwitterionic polymer grafted silica gel via multiple binding sites","authors":"Pengcheng Zhang, Yongxing Hu, Kunling Liu, Yaming Sun, Lijun He, Wenjie Zhao","doi":"10.1002/jssc.202400065","DOIUrl":"10.1002/jssc.202400065","url":null,"abstract":"A novel zwitterionic polymer grafted silica stationary phase, Sil-PZIC, was prepared by bonding poly(ethylene maleic anhydride) molecules on the surface of silica via multiple binding sites, followed by ammonolysis of maleic anhydride through a nucleophilic substitution reaction with ethylenediamine. The stationary phase was characterized by solid-state 13C nuclear magnetic resonance, zeta potential, and elemental analysis and the results show the successful encapsulation of zwitterionic polymer on the surface of silica. The chromatographic performance of Sil-PZIC was investigated by using nucleosides and nucleic bases as test analytes The variation of retention and separation performance of these model compounds were investigated by varying the chromatographic conditions such as the components of mobile phase, salt concentration, and pH. The results show that the retention of the Sil-PZIC phase was dominated by a hydrophilic partitioning mechanism accompanied by secondary interactions such as electrostatic and hydrogen bonding. In addition, saccharides and Amadori compounds were also well separated on the Sil-PZIC, indicating that the Sil-PZIC column has potential application for separation of the polar compound.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 14","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nickel-ion substituted hydroxyapatite matrices for metal-affinity chromatographic purification of recombinant proteins 用于重组蛋白质金属亲和层析纯化的镍离子替代羟基磷灰石基质。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-25 DOI: 10.1002/jssc.202400141

Anbuthangam Ashokan, T. S. Sampath Kumar, Guhan Jayaraman

{"title":"Nickel-ion substituted hydroxyapatite matrices for metal-affinity chromatographic purification of recombinant proteins","authors":"Anbuthangam Ashokan, T. S. Sampath Kumar, Guhan Jayaraman","doi":"10.1002/jssc.202400141","DOIUrl":"10.1002/jssc.202400141","url":null,"abstract":"Hydroxyapatite (HAp) is a calcium phosphate ceramic, widely used as a matrix for protein chromatography. The crystal structure of HAp is amenable to a wide range of substitutions, thus allowing for the alteration of its properties. In this study, nickel-ion substituted HAp (NiSHAp) was synthesized using a wet-precipitation method, followed by spray drying. This resulted in the structural incorporation of nickel ions within well-defined microspheres, which were suitable for chromatographic applications. The chromatographic experiments were conducted with NiSHAp and compared with spray-dried hydroxyapatite (SHAp) matrices. Protein purification experiments were conducted using refolded recombinant L-asparaginase (L-Asp), which was produced as inclusion bodies in Escherichia coli. The results showed that NiSHAp effectively adsorbed L-Asp, which was selectively eluted using a phosphate buffer, surpassing the efficiency of imidazole-based elution. In contrast, SHAp showed weaker binding and lower selectivity. The significance of this study lies in developing a scalable NiSHAp matrix for protein purification, especially for large-scale applications. The NiSHAp matrix offers a cost-effective alternative to commercial immobilized metal affinity chromatography (IMAC) adsorbents, especially for purifying His-tagged proteins. This innovative approach exhibits the advantages of mixed-mode chromatography by combining the properties of hydroxyapatite and IMAC in a single matrix, with the potential of improved industrial-scale protein purification.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 14","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “A microwave-assisted extraction method combined with magnetic ionic liquid-based dispersive liquid–liquid microextraction for the extraction of chloramine–T from fish samples prior to its determination by high-performance liquid chromatography” 更正 "一种微波辅助萃取与磁性离子液体分散液-液微萃取相结合的方法，用于在高效液相色谱法测定氯胺-T之前从鱼类样品中萃取氯胺-T"。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-22 DOI: 10.1002/jssc.202470092

引用次数: 0

Evaluation and comparison of liquid chromatography/high-resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng 评估和比较用于分离和表征人参叶中人参皂苷的液相色谱/高分辨质谱平台。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-22 DOI: 10.1002/jssc.202400354

Xiaohang Li, Yadan Zou, Huizhen Cheng, Mengxiang Ding, Yang Yang, Lili Hong, Ying Xiong, Min Zhang, Xue Li, Qinhua Chen, Hongda Wang, Yuanwu Cui, Wenzhi Yang

{"title":"Evaluation and comparison of liquid chromatography/high-resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng","authors":"Xiaohang Li, Yadan Zou, Huizhen Cheng, Mengxiang Ding, Yang Yang, Lili Hong, Ying Xiong, Min Zhang, Xue Li, Qinhua Chen, Hongda Wang, Yuanwu Cui, Wenzhi Yang","doi":"10.1002/jssc.202400354","DOIUrl":"10.1002/jssc.202400354","url":null,"abstract":"The measurement of data repeatability in small-molecule metabolites acquired within and among different liquid chromatography-mass spectrometry (LC-MS) platforms is crucial for data sharing or data transfer in natural products research. This work was designed to investigate and evaluate the separation and detection performance of three commercial high-resolution LC-MS platforms (e.g., Agilent 6550 QTOF, Waters Vion IM-QTOF, and Thermo Scientific Orbitrap Exploris 120) using 68 ginsenoside references and the extract of Panax ginseng leaf. The retention time (tR), measured on these three platforms (under the same chromatography condition), showed good stability in different concentration tests, and within/among different instruments for both intra-day and inter-day precision examinations. Correlation in tR of ginsenosides was also highly determined on these three platforms. In spite of the different mass analyzers involved, these three platforms gave the accurate mass determination ability, especially enhanced resolution gained because of the ion mobility (IM) separation facilitated by IM-quadrupole time-of-flight. The current study has systematically evaluated the separation and MS detection performance enabled by three high-resolution LC-MS platforms taking ginsenosides as the template, and the reported findings can benefit the researchers for the selection of analytical platforms and the purpose of data sharing or data transfer.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 14","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0