Journal of separation science最新文献_第9页

Indirect and sensitive determination of microRNAs by magnetic field-assisted capillary sieving electrophoresis combined with catalytic hairpin assembly 利用磁场辅助毛细管筛分电泳结合催化发夹组装技术间接灵敏地测定 microRNA。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-21 DOI: 10.1002/jssc.202400166

Jinfeng Ning, Junlan Ye, Qingqing Wang, Wei Wang

{"title":"Indirect and sensitive determination of microRNAs by magnetic field-assisted capillary sieving electrophoresis combined with catalytic hairpin assembly","authors":"Jinfeng Ning, Junlan Ye, Qingqing Wang, Wei Wang","doi":"10.1002/jssc.202400166","DOIUrl":"10.1002/jssc.202400166","url":null,"abstract":"To determine multiple microRNAs (miRNAs) from cells simultaneously is essential for understanding biological functions. Capillary electrophoresis (CE) can simultaneously determine multiple miRNAs by separation. Nevertheless, similar lengths and low concentrations in cells make miRNAs hard to separate and detect. In this study, CE with laser-induced fluorescence detection was combined with catalytic hairpin assembly (CHA) to determine three miRNAs, miR-21, miR-31, and miR-122. The amplification products of CHA, which were DNA duplexes, were designed to have different lengths for different miRNAs. This allowed for easy separation of the duplexes of different miRNAs by CE. The indirect determination of miRNAs was then achieved by separating and detecting these duplexes. A magnetic field was first applied on the capillary sieving electrophoresis to assist in the separation of the duplexes. Under the optimal conditions, the three duplexes could be completely separated within 2.5 min with the detection limits of miRNAs in the range 1.12–4.05 × 10−15 M. MiR-21 and miR-31 were successfully determined from Hela cells, while miR-122 was determined from chicken livers by this method. The recoveries ranged from 97.5% to 118%. The developed method was sensitive and reliable for miRNA determination.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast screening of α-glucosidase inhibitors from Ginkgo biloba leaf by using α-glucosidase immobilized on magnetic metal-organic framework 利用固定在磁性金属有机框架上的α-葡萄糖苷酶快速筛选银杏叶中的α-葡萄糖苷酶抑制剂

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-19 DOI: 10.1002/jssc.202400342

Yue Li, Hongmei Liu, Sikai Wang, Wei Fang, Xinxin Jiang, Guoqi Zhang, Yan Zhao

{"title":"Fast screening of α-glucosidase inhibitors from Ginkgo biloba leaf by using α-glucosidase immobilized on magnetic metal-organic framework","authors":"Yue Li, Hongmei Liu, Sikai Wang, Wei Fang, Xinxin Jiang, Guoqi Zhang, Yan Zhao","doi":"10.1002/jssc.202400342","DOIUrl":"https://doi.org/10.1002/jssc.202400342","url":null,"abstract":"In this study, a ligand fishing method for the screening of α-glucosidase inhibitors from Ginkgo biloba leaf was established for the first time using α-glucosidase immobilized on the magnetic metal-organic framework. The immobilized α-glucosidase exhibited enhanced resistance to temperature and pH, as well as good thermal stability and reusability. Two ligands, namely quercitrin and quercetin, were screened from Ginkgo biloba leaf and identified by ultra-high performance liquid chromatography-tandem mass spectrometry. The half-maximal inhibitory concentration values for quercitrin and quercetin were determined to be 105.69 ± 0.39 and 83.49 ± 0.79 µM, respectively. Molecular docking further confirmed the strong inhibitory effect of these two ligands. The proposed approach in this study demonstrates exceptional efficiency in the screening of α-glucosidase inhibitors from complex natural medicinal plants, thus exhibiting significant potential for the discovery of antidiabetic compounds.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141730301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast screening of α-glucosidase inhibitors from Ginkgo biloba leaf by using α-glucosidase immobilized on magnetic metal-organic framework 利用固定在磁性金属有机框架上的α-葡萄糖苷酶快速筛选银杏叶中的α-葡萄糖苷酶抑制剂

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-19 DOI: 10.1002/jssc.202400342

Yue Li, Hongmei Liu, Sikai Wang, Wei Fang, Xinxin Jiang, Guoqi Zhang, Yan Zhao

{"title":"Fast screening of α-glucosidase inhibitors from Ginkgo biloba leaf by using α-glucosidase immobilized on magnetic metal-organic framework","authors":"Yue Li, Hongmei Liu, Sikai Wang, Wei Fang, Xinxin Jiang, Guoqi Zhang, Yan Zhao","doi":"10.1002/jssc.202400342","DOIUrl":"https://doi.org/10.1002/jssc.202400342","url":null,"abstract":"In this study, a ligand fishing method for the screening of α-glucosidase inhibitors from Ginkgo biloba leaf was established for the first time using α-glucosidase immobilized on the magnetic metal-organic framework. The immobilized α-glucosidase exhibited enhanced resistance to temperature and pH, as well as good thermal stability and reusability. Two ligands, namely quercitrin and quercetin, were screened from Ginkgo biloba leaf and identified by ultra-high performance liquid chromatography-tandem mass spectrometry. The half-maximal inhibitory concentration values for quercitrin and quercetin were determined to be 105.69 ± 0.39 and 83.49 ± 0.79 µM, respectively. Molecular docking further confirmed the strong inhibitory effect of these two ligands. The proposed approach in this study demonstrates exceptional efficiency in the screening of α-glucosidase inhibitors from complex natural medicinal plants, thus exhibiting significant potential for the discovery of antidiabetic compounds.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141730300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparative separation of the constituents from Ginkgo biloba L. leaves by a combination of multi-stage solvent extraction and countercurrent chromatography 结合多级溶剂萃取和逆流色谱法制备分离银杏叶中的成分。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-14 DOI: 10.1002/jssc.202400234

Zhuo Liu, Peng Tian, Luqi Li, Jinxing Guo, Yi Zhou, Shuhui Liu, Zhi Yang

{"title":"Preparative separation of the constituents from Ginkgo biloba L. leaves by a combination of multi-stage solvent extraction and countercurrent chromatography","authors":"Zhuo Liu, Peng Tian, Luqi Li, Jinxing Guo, Yi Zhou, Shuhui Liu, Zhi Yang","doi":"10.1002/jssc.202400234","DOIUrl":"10.1002/jssc.202400234","url":null,"abstract":"In this study, we employed a combination approach for the preparative separation of constituents from Ginkgo biloba L. leaves. It involved multi-stage solvent extractions utilizing two-phase multi-solvent systems and countercurrent chromatography (CCC) separations using three different solvent systems. The n-heptane/ethyl acetate/water (1:1:2, v/v) and n-heptane/ethyl acetate/methanol/water (HepEMWat, 7:3:7:3, v/v) solvent systems were screened out as extraction systems. The polarities of the upper and lower phases in the multi-solvent systems were adjustable, enabling the effectively segmented separation of complex constituents in G. biloba L. The segmented products were subsequently directly utilized as samples and separated using CCC with the solvent systems acetate/n-butanol/water (4:1:5, v/v), HepEMWat (5:5:5:5, v/v), and HepEMWat (9:1:9:1, v/v), respectively. As a result, a total of 11 compounds were successfully isolated and identified from a 2 g methanol extract of G. biloba L through two-stage extraction and three CCC separation processes; among them, nine compounds exhibited high-performance liquid chromatography purity exceeding 85%.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive strategy based on ultra-high performance liquid chromatography with diode array detector fingerprinting and multi-component ultra-performance liquid chromatography with tandem mass spectrometry technology for quality control of Jiawei Huoxiang Zhengqi Pill 基于超高效液相色谱-二极管阵列检测器指纹图谱和多组分超高效液相色谱-串联质谱技术的藿香正气丸质量控制综合策略。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-09 DOI: 10.1002/jssc.202400308

Yang Lu, Ting Lei, Xinyang Chen, Na Ning, Qiuling Huang, Xu Wu, Shengpeng Wang, Peng Li, Li Wan, Jiliang Cao

{"title":"A comprehensive strategy based on ultra-high performance liquid chromatography with diode array detector fingerprinting and multi-component ultra-performance liquid chromatography with tandem mass spectrometry technology for quality control of Jiawei Huoxiang Zhengqi Pill","authors":"Yang Lu, Ting Lei, Xinyang Chen, Na Ning, Qiuling Huang, Xu Wu, Shengpeng Wang, Peng Li, Li Wan, Jiliang Cao","doi":"10.1002/jssc.202400308","DOIUrl":"10.1002/jssc.202400308","url":null,"abstract":"Jiawei Huoxiang Zhengqi Pill (JHZP) is a commonly used Chinese patent medicine for the clinical treatment of headache, dizziness, chest tightness as well as abdominal distension, and pain caused by wind-cold flu. In this study, a comprehensive strategy combining ultra-high performance liquid chromatography with diode array detector (UHPLC-DAD) fingerprinting and multi-component quantitative analysis was established and validated for quality evaluation of JHZP. A total of 49 characteristic common peaks were selected in a chromatographic fingerprinting study to assess the similarity of 15 batches of JHZP. Furthermore, 109 compounds were identified or preliminarily identified from JHZP by coupling with an advanced hybrid linear ion trap-Orbitrap mass spectrometer. For quantification, the optimized ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was employed for the simultaneous determination of 13 target compounds within 12 min. The sensitivity, precision, reproducibility, and accuracy of the method were satisfactory. This validated UPLC-MS/MS method was successfully applied to analyzing 15 batches of JHZP. The proposed comprehensive strategy combining UHPLC-DAD fingerprinting and multi-component UPLC-MS/MS analysis proved to be highly efficient, accurate, and reliable for the quality evaluation of JHZP, which can be considered as a reference for the overall quality evaluation of other Chinese herbal formulations.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis 层流剖面横向扩散方法在毛细管电泳毛细管内反应中的非典型应用。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-09 DOI: 10.1002/jssc.202400157

Taťána Bržezická, Lenka Kohútová, Zdeněk Glatz

{"title":"Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis","authors":"Taťána Bržezická, Lenka Kohútová, Zdeněk Glatz","doi":"10.1002/jssc.202400157","DOIUrl":"10.1002/jssc.202400157","url":null,"abstract":"Capillary electrophoresis (CE) is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterized by many well-known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked. It is the possibility to perform chemical and biochemical reactions at the nL scale inside the separation capillary. There are two basic formats applicable for this purpose, heterogeneous and homogeneous. In the former, one reactant is immobilized onto a particle or monolithic support or directly on the capillary wall, and the other is injected. In the latter, the reactant mixing inside a capillary is based on electromigration or diffusion. One of the diffusion-based methodologies, termed Transverse Diffusion of Laminar Flow Profiles, is the subject of this review. Since most studies utilizing in-capillary reactions in CE focus on enzymes, which are being continuously and exhaustively reviewed, this review covers the atypical applications of this methodology, but still in the bioanalytical field. As can be seen from the demonstrated applications, they are not limited to reactions, but can also be utilized for other biochemical systems.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of amino acids secreted by yeast cells by hydrophilic interaction liquid chromatography-tandem mass spectrometry 利用亲水相互作用液相色谱-串联质谱法对酵母细胞分泌的氨基酸进行定量。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-09 DOI: 10.1002/jssc.202400318

Xiongwei Yin, Luís Santos Sousa, Bruno André, Erwin Adams, Ann Van Schepdael

{"title":"Quantification of amino acids secreted by yeast cells by hydrophilic interaction liquid chromatography-tandem mass spectrometry","authors":"Xiongwei Yin, Luís Santos Sousa, Bruno André, Erwin Adams, Ann Van Schepdael","doi":"10.1002/jssc.202400318","DOIUrl":"10.1002/jssc.202400318","url":null,"abstract":"Monitoring the levels of amino acids (AAs) in biological cell cultures provides key information to understand the regulation of cell growth and metabolism. Saccharomyces cerevisiae can naturally excrete AAs, making accurate detection and determination of amino acid levels within the cultivation medium pivotal for gaining insights into this still poorly known process. Given that most AAs lack ultraviolet (UV) chromophores or fluorophores necessary for UV and fluorescence detection, derivatization is commonly utilized to enhance amino acid detectability via UV absorption. Unfortunately, this can lead to drawbacks such as derivative instability, labor intensiveness, and poor reproducibility. Hence, this study aimed to develop an accurate and stable hydrophilic interaction liquid chromatography-tandem mass spectrometry analytical method for the separation of all 20 AAs within a short 17-min run time. The method provides satisfactory linearity and sensitivity for all analytes. The method has been validated for intra- and inter-day precision, accuracy, recovery, matrix effect, and stability. It has been successfully applied to quantify 20 AAs in samples of yeast cultivation medium. This endeavor seeks to enhance our comprehension of amino acid profiles in the context of cell growth and metabolism within yeast cultivation media.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress for chiral stationary phases based on chiral porous materials in high-performance liquid chromatography and gas chromatography separation 基于手性多孔材料的手性固定相在高效液相色谱和气相色谱分离领域的最新进展。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-05 DOI: 10.1002/jssc.202400073

Zhen Wang, Wei Wang, Ai-Qin Luo, Li-Ming Yuan

引用次数: 0

One-step enrichment and stepwise elution of glycoproteins and phosphoproteins by hydrophilic Ti4+-immobilized dendrimer poly(glycidyl methacrylate) microparticles functionalized with polyethylenimine and phytic acid 用聚乙烯亚胺和植酸官能化的亲水性 Ti4+-immobilized 树枝状聚（甲基丙烯酸缩水甘油酯）微粒一步富集和逐步洗脱糖蛋白和磷蛋白。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-07-01 DOI: 10.1002/jssc.202400154

Hao Wang, Simin Li, Xinyan Wang, Xinyue Li, Minzhi Jiang, Shiying Chen, Zaiqi Hu, Haitian Li, Yuzhen Xu, Lei Jin

{"title":"One-step enrichment and stepwise elution of glycoproteins and phosphoproteins by hydrophilic Ti4+-immobilized dendrimer poly(glycidyl methacrylate) microparticles functionalized with polyethylenimine and phytic acid","authors":"Hao Wang, Simin Li, Xinyan Wang, Xinyue Li, Minzhi Jiang, Shiying Chen, Zaiqi Hu, Haitian Li, Yuzhen Xu, Lei Jin","doi":"10.1002/jssc.202400154","DOIUrl":"10.1002/jssc.202400154","url":null,"abstract":"Glycosylation and phosphorylation rank as paramount post-translational modifications, and their analysis heavily relies on enrichment techniques. In this work, a facile approach was developed for the one-step simultaneous enrichment and stepwise elution of glycoproteins and phosphoproteins. The core of this approach was the application of the novel titanium (IV) ion immobilized poly(glycidyl methacrylate) microparticles functionalized with dendrimer polyethylenimine and phytic acid. The microparticles possessed dual enrichment capabilities due to their abundant titanium ions and hydroxyl groups on the surface. They demonstrate rapid adsorption equilibrium (within 30 min) and exceptional adsorption capacity for β-casein (1107.7 mg/g) and horseradish peroxidase (438.6 mg/g), surpassing that of bovine serum albumin (91.7 mg/g). Furthermore, sodium dodecyl sulfate-polyacrylamide gel electrophoresis was conducted to validate the enrichment capability. Experimental results across various biological samples, including standard protein mixtures, non-fat milk, and human serum, demonstrated the remarkable ability of these microparticles to enrich low-abundance glycoproteins and phosphoproteins from biological samples.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring separation mechanisms and lipophilicity in hydrophilic interaction chromatography conditions by thin-layer chromatography of anesthetics and adjuvant drugs as polar model compounds 以极性模型化合物麻醉剂和辅助药物的薄层色谱法探索亲水相互作用色谱条件下的分离机制和亲油性。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-06-27 DOI: 10.1002/jssc.202400099

Aleksandra Radoičić, Sandra Šegan, Dušanka Milojković-Opsenica

{"title":"Exploring separation mechanisms and lipophilicity in hydrophilic interaction chromatography conditions by thin-layer chromatography of anesthetics and adjuvant drugs as polar model compounds","authors":"Aleksandra Radoičić, Sandra Šegan, Dušanka Milojković-Opsenica","doi":"10.1002/jssc.202400099","DOIUrl":"10.1002/jssc.202400099","url":null,"abstract":"The chromatographic behavior of the selected compounds was studied under conditions of hydrophilic interaction liquid chromatography (HILIC). The effect of mobile phase composition on the retention in different chromatographic systems was systematically examined using high-performance thin-layer chromatography. The sorbents of different polarity and adsorption characteristics were selected and mixtures of water and organic solvents of various compositions, from pure water to pure organic solvent were used as mobile phases. Increasing the amount of water in the mobile phase leads to a conversion of the separation mechanism, and the retention curves have a characteristic “U” shape. The conversion between the adsorption and partition mechanisms is most likely continuous and depends on the chemical nature of separated substances, the stationary phase as well as on organic component of the mobile phase. Silica gel can be considered the most suitable stationary phase for the systematic investigation of the chromatographic behavior of the test compounds, whereas acetonitrile was the most suitable solvent. The obtained results contribute to the understanding of the dominant separation mechanism, the type, and the intensity of the interactions between separated substances with both stationary and mobile phases. Besides, the lipophilicity parameters obtained under HILIC conditions were evaluated and correlated with the calculated values.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0