Journal of separation science最新文献_第8页

Robust In Situ Growth of Epitaxially Oriented Titanium Dioxide Microplates on Titanium Fiber for Selective and Efficient Solid-Phase Microextraction of Polycyclic Aromatic Hydrocarbons From Water Samples Prior to HPLC-UV 外延定向二氧化钛微孔板在钛纤维上的原位生长，用于高效固相萃取水样中的多环芳烃

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-22 DOI: 10.1002/jssc.70196

Jiayu Li, Huirong Li, Jinxin Guo, Hua Zhou, Xuemei Wang, Xinzhen Du

{"title":"Robust In Situ Growth of Epitaxially Oriented Titanium Dioxide Microplates on Titanium Fiber for Selective and Efficient Solid-Phase Microextraction of Polycyclic Aromatic Hydrocarbons From Water Samples Prior to HPLC-UV","authors":"Jiayu Li, Huirong Li, Jinxin Guo, Hua Zhou, Xuemei Wang, Xinzhen Du","doi":"10.1002/jssc.70196","DOIUrl":"https://doi.org/10.1002/jssc.70196","url":null,"abstract":"<div>\u0000 \u0000 <p>Well-defined TiO<sub>2</sub> microplates (TiO<sub>2</sub>MPs) were directly achieved on a titanium fiber by the hydrothermal treatment in alkaline solution. The hydrothermal in situ growth of TiO<sub>2</sub>MPs greatly depended on the alkali concentration and hydrothermal temperature. The results proved the formation of epitaxially oriented TiO<sub>2</sub>MPs with rutile structure. The Ti@TiO<sub>2</sub>MPs fiber exhibited improved adsorption selectivity and enhanced adsorption capability for PAHs. After optimizing adsorption conditions, the developed method with Ti@TiO<sub>2</sub>MPs fiber presented good linearity of PAHs in the ranges of 0.05–200 ng/mL with LODs of 0.012–0.161 ng/mL and LOQs of 0.039–0.537 ng/mL. The intra-day and inter-day RSDs for the developed method with a single fiber were 2.59%–4.32% and 3.29%–6.31%, respectively. Moreover, RSDs were found to be 5.48%–8.35% for the developed method with five pieces of fibers fabricated in different batches. Finally, the developed method was applied to selective preconcentration and sensitive determination of target PAHs in environmental water samples. The relative recoveries of target PAHs in real water samples spiked at the concentrations of 5.0 and 10.0 ng/mL were in the ranges of 87.20%–110.6% with RSDs lower than 7.16%. Furthermore, the Ti@TiO<sub>2</sub>MPs fiber was highly stable without loss of adsorption efficiency after 200 adsorption and desorption cycles.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic Molecularly Imprinted Polymers for Extraction of Four Amphetamine-Type Stimulants From Sewage and Urine 磁性分子印迹聚合物从污水和尿液中提取四种安非他明类兴奋剂

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-22 DOI: 10.1002/jssc.70197

Haocheng Fan, Dongqin Tan, Yue Wang, Yanying Li, Degao Wang

{"title":"Magnetic Molecularly Imprinted Polymers for Extraction of Four Amphetamine-Type Stimulants From Sewage and Urine","authors":"Haocheng Fan, Dongqin Tan, Yue Wang, Yanying Li, Degao Wang","doi":"10.1002/jssc.70197","DOIUrl":"https://doi.org/10.1002/jssc.70197","url":null,"abstract":"<div>\u0000 \u0000 <p>Magnetic molecularly imprinted polymers (MMIPs) were synthesized by bulk polymerization using N-methylphenethylamine as a dummy template for selective extraction of four amphetamine-type stimulants (ATSs) in sewage and urine. The MMIPs were characterized by scanning electron microscopy, vibrating sample magnetometer, Fourier transform infrared spectroscopy and thermogravimetric analysis, revealing a porous structure (35–60 µm) with a saturation magnetization of 1.8 emu/g, enabling rapid magnetic separation within 1 min. Adsorption kinetics followed a pseudo-second-order model, achieving over 80% adsorption within 10 min and an imprinting factor of 2.4–3.3, demonstrating high selectivity for ATSs over structural analogs. Optimized magnetic solid-phase extraction (MSPE) conditions yielded recoveries over 81% with relative standard deviation (RSD) below 5.5%. Coupled with high performance liquid chromatography-tandem mass spectrometry, the proposed MMIPs–MSPE method exhibited a linear range of 1–100 ng/mL, low detection limits (5.2–19 ng/L), and quantification limits (17.0–65.0 ng/L). The pre-concentration factor of ATSs was 19.3–19.9. Successful application to real sewage and urine samples confirmed robust matrix interference resistance and reliability (recoveries: 82.1%–116%, RSD < 6.4%). This study provides a sensitive, reusable, and environmentally friendly approach for monitoring ATSs in environmental and forensic matrices.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of the High-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Creatine, L-Carnitine, and Creatinine in Tears 高效液相色谱-串联质谱法测定泪液中肌酸、左旋肉碱和肌酐的建立

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-11 DOI: 10.1002/jssc.70193

Helena Jurdáková, Michaela Šimurdová, Renáta Górová

{"title":"Development of the High-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Creatine, L-Carnitine, and Creatinine in Tears","authors":"Helena Jurdáková, Michaela Šimurdová, Renáta Górová","doi":"10.1002/jssc.70193","DOIUrl":"https://doi.org/10.1002/jssc.70193","url":null,"abstract":"<div>\u0000 \u0000 <p>The aim of this work was to develop the HPLC-MS/MS method for the determination of L-carnitine and creatine in tears, as these molecules can be used as biomarkers of several diseases and health conditions with neurological manifestation, simultaneously with the determination of creatinine as normalization factor.</p>\u0000 <p>Tears were collected using a microtube during crying or using Schirmer strip by inserting it under the lower eyelid. Due to the low stability of creatine, only a few hours at room temperature, it is advisable to freeze the samples immediately after collection. Different methanol to water contents in the extraction solution were tested (0%–100%). Extraction recovery from Schirmer strips, matrix effect, and overall process efficiency were evaluated for 0.3 and 1 mL of extraction solution used. Finally, the extraction into 1 mL of 80% methanol containing 0.1% formic acid, and subsequent HPLC-MS/MS separation at isocratic conditions (methanol:water 5:95% with 0.1% formic acid and flow rate of 0.25 mL/min) using a Synergi 4 μ Polar-RP column (150 × 2 mm) and detection in the positive ion mode was used. The limits of quantifications for L-carnitine, creatine, and creatinine in tears were 0.5, 1.0, and 1.0 µmol/L, respectively. Intraday and interday precisions were up to 4.4% and bias did not exceed 12% for all analytes. Linearity was tested and approved in the range of 0.23–30 µmol/L for L-carnitine and 0.78–100 µmol/L for creatine and creatinine. Measured values of L-carnitine, creatine, and creatinine in real tear samples of healthy individuals (<i>n</i> = 7) were within the range of 1.1–8.2, 4.8–46.3, and 8–44.7 µmol/L, respectively. Obtained results were compared with published results in tears if available (L-carnitine and creatinine) or with other biological matrices. As our knowledge, this publication is the first that reports the content of creatine in tears.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of the High-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Creatine, L-Carnitine, and Creatinine in Tears 高效液相色谱-串联质谱法测定泪液中肌酸、左旋肉碱和肌酐的建立

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-11 DOI: 10.1002/jssc.70193

Helena Jurdáková, Michaela Šimurdová, Renáta Górová

{"title":"Development of the High-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Creatine, L-Carnitine, and Creatinine in Tears","authors":"Helena Jurdáková, Michaela Šimurdová, Renáta Górová","doi":"10.1002/jssc.70193","DOIUrl":"https://doi.org/10.1002/jssc.70193","url":null,"abstract":"<div>\u0000 \u0000 <p>The aim of this work was to develop the HPLC-MS/MS method for the determination of L-carnitine and creatine in tears, as these molecules can be used as biomarkers of several diseases and health conditions with neurological manifestation, simultaneously with the determination of creatinine as normalization factor.</p>\u0000 <p>Tears were collected using a microtube during crying or using Schirmer strip by inserting it under the lower eyelid. Due to the low stability of creatine, only a few hours at room temperature, it is advisable to freeze the samples immediately after collection. Different methanol to water contents in the extraction solution were tested (0%–100%). Extraction recovery from Schirmer strips, matrix effect, and overall process efficiency were evaluated for 0.3 and 1 mL of extraction solution used. Finally, the extraction into 1 mL of 80% methanol containing 0.1% formic acid, and subsequent HPLC-MS/MS separation at isocratic conditions (methanol:water 5:95% with 0.1% formic acid and flow rate of 0.25 mL/min) using a Synergi 4 μ Polar-RP column (150 × 2 mm) and detection in the positive ion mode was used. The limits of quantifications for L-carnitine, creatine, and creatinine in tears were 0.5, 1.0, and 1.0 µmol/L, respectively. Intraday and interday precisions were up to 4.4% and bias did not exceed 12% for all analytes. Linearity was tested and approved in the range of 0.23–30 µmol/L for L-carnitine and 0.78–100 µmol/L for creatine and creatinine. Measured values of L-carnitine, creatine, and creatinine in real tear samples of healthy individuals (<i>n</i> = 7) were within the range of 1.1–8.2, 4.8–46.3, and 8–44.7 µmol/L, respectively. Obtained results were compared with published results in tears if available (L-carnitine and creatinine) or with other biological matrices. As our knowledge, this publication is the first that reports the content of creatine in tears.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Temperature Liquid Chromatography of Isomeric Organochlorine Herbicides and Their Degradation Products 异构体有机氯除草剂及其降解产物的高温液相色谱分析

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-10 DOI: 10.1002/jssc.70191

Michal Kašpar, Petra Peroutková, Jiří Palarčík, Petr Česla

{"title":"High-Temperature Liquid Chromatography of Isomeric Organochlorine Herbicides and Their Degradation Products","authors":"Michal Kašpar, Petra Peroutková, Jiří Palarčík, Petr Česla","doi":"10.1002/jssc.70191","DOIUrl":"https://doi.org/10.1002/jssc.70191","url":null,"abstract":"<div>\u0000 \u0000 <p>Alachlor, acetochlor, and metolachlor are organochlorine herbicides extensively used for weed control in agricultural practices, yet their use is prohibited within the European Union due to environmental and health concerns. These compounds and their degradation products exhibit high environmental persistence, necessitating reliable and rapid analytical methods for monitoring. Conventional reversed-phase liquid chromatography employing C18 columns is commonly utilized for their analysis. However, alkyl silica columns, and especially C18 columns, are not suitable for the effective separation of isomers, necessitating the integration of tandem mass spectrometry for enhanced analytical accuracy. This study explores the application of a porous graphitic carbon-based column to overcome these limitations. High-temperature chromatography enabled improved efficiency and selectivity in separating the target compounds. The influence of temperature, flow rate, pH, and mobile phase composition on the retention of alachlor, acetochlor, metolachlor, and their degradation products was systematically evaluated. A method suitable for separation and quantification of the herbicides was developed, highlighting the potential of porous graphitic carbon columns for robust and precise analysis in complex environmental matrices.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LC-MS/MS of Permethylated O-Glycans, Free Oligosaccharides, and Glycosphingolipid Glycans Using Mesoporous Graphitized Carbon Column 介孔石墨化碳柱对过甲基化o -聚糖、游离低聚糖和鞘脂聚糖的LC-MS/MS分析

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-10 DOI: 10.1002/jssc.70187

Oluwatosin Daramola, Sakshi Gautam, Andrew I. Bennett, Mona Goli, Cristian D. Guiterrez-Reyes, Junyao Wang, Yehia Mechref

{"title":"LC-MS/MS of Permethylated O-Glycans, Free Oligosaccharides, and Glycosphingolipid Glycans Using Mesoporous Graphitized Carbon Column","authors":"Oluwatosin Daramola, Sakshi Gautam, Andrew I. Bennett, Mona Goli, Cristian D. Guiterrez-Reyes, Junyao Wang, Yehia Mechref","doi":"10.1002/jssc.70187","DOIUrl":"https://doi.org/10.1002/jssc.70187","url":null,"abstract":"<div>\u0000 \u0000 <p>Glycosylation, a common post-translational modification, is crucial in various biological processes and diseases. While <i>N</i>-linked glycans have been widely studied, <i>O</i>-linked glycans and other small glycan structures, such as glycosphingolipid (GSL) glycans and free oligosaccharides, have been less explored due to their structural complexity and low abundance. To address the challenge of isomeric separation for these small glycans, we employed a mesoporous graphitized carbon (MGC) column, which offers an effective alternative to the nano-porous graphitized carbon (PGC) column. Previously, the MGC column demonstrated efficient isomeric separation of <i>N-</i>glycans and glycopeptides. In this study, we optimized chromatographic conditions using standard samples to extend the application of the MGC column to <i>O</i>-glycans, free oligosaccharides, and GSL glycans. These optimizations resulted in improved retention and isomeric separation. The method was validated through successful isomeric separation of <i>O</i>-glycans, free oligosaccharides, and GSL glycans derived from human milk, showcasing the MGC column's potential for comprehensive glycomics analysis of biological samples. This study underscores the robustness and reproducibility of the MGC column, highlighting its suitability for advanced glycomics, particularly in biological samples where <i>O</i>-glycans, GSL glycans, or free oligosaccharides play significant roles in disease progression.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LC-MS/MS of Permethylated O-Glycans, Free Oligosaccharides, and Glycosphingolipid Glycans Using Mesoporous Graphitized Carbon Column 介孔石墨化碳柱对过甲基化o -聚糖、游离低聚糖和鞘脂聚糖的LC-MS/MS分析

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-10 DOI: 10.1002/jssc.70187

Oluwatosin Daramola, Sakshi Gautam, Andrew I. Bennett, Mona Goli, Cristian D. Guiterrez-Reyes, Junyao Wang, Yehia Mechref

{"title":"LC-MS/MS of Permethylated O-Glycans, Free Oligosaccharides, and Glycosphingolipid Glycans Using Mesoporous Graphitized Carbon Column","authors":"Oluwatosin Daramola, Sakshi Gautam, Andrew I. Bennett, Mona Goli, Cristian D. Guiterrez-Reyes, Junyao Wang, Yehia Mechref","doi":"10.1002/jssc.70187","DOIUrl":"https://doi.org/10.1002/jssc.70187","url":null,"abstract":"<div>\u0000 \u0000 <p>Glycosylation, a common post-translational modification, is crucial in various biological processes and diseases. While <i>N</i>-linked glycans have been widely studied, <i>O</i>-linked glycans and other small glycan structures, such as glycosphingolipid (GSL) glycans and free oligosaccharides, have been less explored due to their structural complexity and low abundance. To address the challenge of isomeric separation for these small glycans, we employed a mesoporous graphitized carbon (MGC) column, which offers an effective alternative to the nano-porous graphitized carbon (PGC) column. Previously, the MGC column demonstrated efficient isomeric separation of <i>N-</i>glycans and glycopeptides. In this study, we optimized chromatographic conditions using standard samples to extend the application of the MGC column to <i>O</i>-glycans, free oligosaccharides, and GSL glycans. These optimizations resulted in improved retention and isomeric separation. The method was validated through successful isomeric separation of <i>O</i>-glycans, free oligosaccharides, and GSL glycans derived from human milk, showcasing the MGC column's potential for comprehensive glycomics analysis of biological samples. This study underscores the robustness and reproducibility of the MGC column, highlighting its suitability for advanced glycomics, particularly in biological samples where <i>O</i>-glycans, GSL glycans, or free oligosaccharides play significant roles in disease progression.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Temperature Liquid Chromatography of Isomeric Organochlorine Herbicides and Their Degradation Products 异构体有机氯除草剂及其降解产物的高温液相色谱分析

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-10 DOI: 10.1002/jssc.70191

Michal Kašpar, Petra Peroutková, Jiří Palarčík, Petr Česla

{"title":"High-Temperature Liquid Chromatography of Isomeric Organochlorine Herbicides and Their Degradation Products","authors":"Michal Kašpar, Petra Peroutková, Jiří Palarčík, Petr Česla","doi":"10.1002/jssc.70191","DOIUrl":"https://doi.org/10.1002/jssc.70191","url":null,"abstract":"<div>\u0000 \u0000 <p>Alachlor, acetochlor, and metolachlor are organochlorine herbicides extensively used for weed control in agricultural practices, yet their use is prohibited within the European Union due to environmental and health concerns. These compounds and their degradation products exhibit high environmental persistence, necessitating reliable and rapid analytical methods for monitoring. Conventional reversed-phase liquid chromatography employing C18 columns is commonly utilized for their analysis. However, alkyl silica columns, and especially C18 columns, are not suitable for the effective separation of isomers, necessitating the integration of tandem mass spectrometry for enhanced analytical accuracy. This study explores the application of a porous graphitic carbon-based column to overcome these limitations. High-temperature chromatography enabled improved efficiency and selectivity in separating the target compounds. The influence of temperature, flow rate, pH, and mobile phase composition on the retention of alachlor, acetochlor, metolachlor, and their degradation products was systematically evaluated. A method suitable for separation and quantification of the herbicides was developed, highlighting the potential of porous graphitic carbon columns for robust and precise analysis in complex environmental matrices.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the (lipo)Phenolic Composition of Olive Oil By-Products and Biomass From Three Different Olea Europaea Cultivars by Liquid Chromatography Coupled to Photodiode Array and Mass Spectrometry 液相色谱-光电二极管阵列和质谱联用分析三种不同欧洲油橄榄品种橄榄油副产物和生物量的脂质酚成分

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-10 DOI: 10.1002/jssc.70189

Francesco Cacciola, Wadir Mario Valentino Marchesiello, Micaela Galletta, Carmelo Coppolino, Marina Russo, Antonella Buccafuri, Alessia Fazio, Pierluigi Plastina, Maurizio Quinto, Paola Dugo, Luigi Mondello

{"title":"Elucidating the (lipo)Phenolic Composition of Olive Oil By-Products and Biomass From Three Different Olea Europaea Cultivars by Liquid Chromatography Coupled to Photodiode Array and Mass Spectrometry","authors":"Francesco Cacciola, Wadir Mario Valentino Marchesiello, Micaela Galletta, Carmelo Coppolino, Marina Russo, Antonella Buccafuri, Alessia Fazio, Pierluigi Plastina, Maurizio Quinto, Paola Dugo, Luigi Mondello","doi":"10.1002/jssc.70189","DOIUrl":"https://doi.org/10.1002/jssc.70189","url":null,"abstract":"<p>The olive oil industry is currently experiencing a period of significant growth, largely as a result of the high value placed on olive oil as a high-nutritional food product on a global scale. Industrial olive oil production generates significant quantities of solid waste and dark liquid effluents, involving olive leaves (OLs), olive pomace (OP), and olive mill wastewaters (OMWWs). Particular attention was given to the recycling and valorization of by-products and biomass from the olive oil industry due to their richness in bioactive compounds, including phenolic compounds with antioxidant properties. The aim of this study was the sustainable recovery and characterization of valuable biocompounds, notably phenols, with particular emphasis on lipophenols, from different matrices (OMWWs, OP, and OLs) of three different <i>Olea europaea</i> cultivars using LC coupled with photodiode array (PDA) and MS detection. Method validation demonstrated satisfactory linearity (<i>R</i><sup>2</sup> > 0.9806), repeatability (lower than 9.82%) and recovery (over 79.07%) for all samples investigated. Before quantification, the matrix effect was also evaluated with values ranging from 71.99% to 139.70%.The results highlight that OLs, OP, and OMWWs might be employed for several applications, including nutraceuticals, cosmeceuticals, food, and animal feed.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the (lipo)Phenolic Composition of Olive Oil By-Products and Biomass From Three Different Olea Europaea Cultivars by Liquid Chromatography Coupled to Photodiode Array and Mass Spectrometry 液相色谱-光电二极管阵列和质谱联用分析三种不同欧洲油橄榄品种橄榄油副产物和生物量的脂质酚成分

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-06-10 DOI: 10.1002/jssc.70189

Francesco Cacciola, Wadir Mario Valentino Marchesiello, Micaela Galletta, Carmelo Coppolino, Marina Russo, Antonella Buccafuri, Alessia Fazio, Pierluigi Plastina, Maurizio Quinto, Paola Dugo, Luigi Mondello

{"title":"Elucidating the (lipo)Phenolic Composition of Olive Oil By-Products and Biomass From Three Different Olea Europaea Cultivars by Liquid Chromatography Coupled to Photodiode Array and Mass Spectrometry","authors":"Francesco Cacciola, Wadir Mario Valentino Marchesiello, Micaela Galletta, Carmelo Coppolino, Marina Russo, Antonella Buccafuri, Alessia Fazio, Pierluigi Plastina, Maurizio Quinto, Paola Dugo, Luigi Mondello","doi":"10.1002/jssc.70189","DOIUrl":"https://doi.org/10.1002/jssc.70189","url":null,"abstract":"<p>The olive oil industry is currently experiencing a period of significant growth, largely as a result of the high value placed on olive oil as a high-nutritional food product on a global scale. Industrial olive oil production generates significant quantities of solid waste and dark liquid effluents, involving olive leaves (OLs), olive pomace (OP), and olive mill wastewaters (OMWWs). Particular attention was given to the recycling and valorization of by-products and biomass from the olive oil industry due to their richness in bioactive compounds, including phenolic compounds with antioxidant properties. The aim of this study was the sustainable recovery and characterization of valuable biocompounds, notably phenols, with particular emphasis on lipophenols, from different matrices (OMWWs, OP, and OLs) of three different <i>Olea europaea</i> cultivars using LC coupled with photodiode array (PDA) and MS detection. Method validation demonstrated satisfactory linearity (<i>R</i><sup>2</sup> > 0.9806), repeatability (lower than 9.82%) and recovery (over 79.07%) for all samples investigated. Before quantification, the matrix effect was also evaluated with values ranging from 71.99% to 139.70%.The results highlight that OLs, OP, and OMWWs might be employed for several applications, including nutraceuticals, cosmeceuticals, food, and animal feed.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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