Journal of separation science最新文献

{"title":"Targeting the Antibody Fab Region Using Light-Induced Indole-3-Butyric Acid Functionalized Magnetic Microspheres","authors":"Yu Yi,&nbsp;Yao Li,&nbsp;Sa Wang,&nbsp;Yuting Liang,&nbsp;Jianfeng Mei,&nbsp;Guoqing Ying","doi":"10.1002/jssc.70086","DOIUrl":"https://doi.org/10.1002/jssc.70086","url":null,"abstract":"<div>\u0000 \u0000 <p>A novel light-controlled adsorption system for direct targeting of antibody Fab fragments was developed by utilizing indole-3-butyric acid functionalized magnetic microspheres. Indole-3-butyric acid, serving as a specific small molecule ligand, was successfully conjugated to amine-functionalized magnetic microspheres via a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/<i>N</i>-hydroxysuccinimide activation strategy. Under illumination at a particular wavelength, the indole-3-butyric acid ligand generated reactive radicals that interacted with the nucleotide-binding sites of antibody Fab fragments, enabling effective affinity adsorption. Static adsorption experiments demonstrated that the system's adsorption behavior obeys the Langmuir model (<i>K</i>_F = 0.122, <i>R</i>² = 0.996), indicating a homogeneous adsorption process. Kinetic studies further revealed that the adsorption process follows a second-order kinetic model (<i>k</i>₂ = 0.0257, <i>R</i>² = 0.989). When compared with conventional antibody adsorption systems, this new system exhibited specific targeting of Fab fragments, enhanced selectivity, and adjustable properties. In particular, at pH 7.0, effective elution was achieved by increasing the salt concentration, with the eluted product retaining antigen-binding activity. The purification recovery rate exceeded 98%, and the system maintained effective adsorption and elution of Fab fragments across various pH conditions. Besides, even after 10 reuse cycles, the system retained more than 96% of its efficiency, thus presenting excellent regenerability and reusability. In summary, the developed light-controlled antibody Fab region adsorption system offers a highly efficient, stable, and cost-effective approach. It is also expected to become one of the most effective methods for antibody Fab purification in the future.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation and Purification of Ginsenosides and Flavonoids in From the Leaves and Stems of Panax quinquefolium by High-Speed Countercurrent Chromatography and Online-Storage Inner-Recycling Countercurrent Chromatography","authors":"Chuangchuang Wang,&nbsp;Jinqian Yu,&nbsp;Yingjian Guo,&nbsp;Min Jiang,&nbsp;Kai Zhong,&nbsp;Xiao Wang","doi":"10.1002/jssc.70073","DOIUrl":"https://doi.org/10.1002/jssc.70073","url":null,"abstract":"<div>\u0000 \u0000 <p>Study aimed to isolate and purify compounds from the stems and leaves of <i>Panax quinquefolius</i>. By employing a highly innovative separation technique that combined multistage countercurrent chromatography (MRCC), high-speed countercurrent chromatography (HSCCC), and an advanced online-storage inner-recycling countercurrent chromatography (OS-IRCCC) mode for the first time, 12 compounds were successfully isolated, including 10 ginsenosides and 2 flavonoids. First, the crude extract was fractionated into five parts using D101 MRCC, with HPLC analysis revealing that 40% and 60% ethanol eluate contained the highest compound diversity. Overall, 40% ethanol eluate was separated using the solvent system of EtOAc/<i>n</i>-BuOH/H₂O (2:1:3, v/v), whereas 60% ethanol eluate underwent traditional countercurrent chromatography coupled with OS-IRCCC separation using the solvent system of methyl tert-butyl ether (MTBE)/<i>n</i>-BuOH/ACN/H₂O (4:2:3:8, v/v). Ultimately, various compounds were obtained, including kaempferol 3-<i>O</i>-<i>β</i>-<span>d</span>-glucopyranosyl-(1 → 2)-<i>β</i>-<span>d</span>-galactopyranosyl-7-<i>O</i>-<i>α</i>-<span>l</span>-rhamnopyranoside (13.2 mg), ginsenoside Rc (7.4 mg), 20(R)-ginsenoside Rh₁ (7.2 mg), ginsenoside Re (12.3 mg), kaempferol 3-<i>O</i>-<i>β</i>-<span>d</span>-glucopyranosyl-(1 → 2)-<i>β</i>-<span>d</span>-galactopyranoside (14.1 mg), ginsenoside Rb₁ (8.2 mg), ginsenoside Rb₂ (17.5 mg), ginsenoside Rb₃ (27.3 mg), ginsenoside Rg₁ (13.3 mg), ginsenoside Rg₂ (9.7 mg), ginsenoside Rd (11.4 mg), and pseudo-ginsenoside F₁₁ (16.7 mg). This research highlights the efficacy of the novel separation technique in isolating and purifying valuable compounds from <i>P. quinquefolius</i> stems and leaves.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Consistency Evaluation of Chemical Composition and Pharmacology of Pheretima aspergillum Dispensing Granules and Traditional Decoction Based on HPLC, GC-IMS, and Animal Experiments","authors":"Han Li,&nbsp;Xiaoming Wu,&nbsp;Yan Miao,&nbsp;Fengyu Dong,&nbsp;Xinjing Gui,&nbsp;Panpan Wang,&nbsp;Xuelin Li,&nbsp;Ruixin Liu","doi":"10.1002/jssc.70090","DOIUrl":"https://doi.org/10.1002/jssc.70090","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigates the consistency of quality between the traditional decoction (TD) of <i>Pheretima aspergillum</i> and its dispensing granule decoction (DGD) by examining their chemical composition and antithrombotic efficacy. We first determined the nucleoside components, protein content, and volatile compounds in 10 batches of TD and 9 batches of DGD sourced from three manufacturers using high-performance liquid chromatography (HPLC), UV–vis spectrophotometry, and gas chromatography ion mobility spectrometry (GC-IMS). Next, we assessed antithrombotic efficacy by measuring the relative length of tail thrombosis and calculating the thrombus inhibition rates at 24 and 48 h. Our comprehensive analysis revealed that the concentrations of hypoxanthine, uridine, inosine, adenosine, and proteins in TD were significantly higher than those in DGD. Moreover, the types and concentrations of volatile compounds in TD and DGD exhibited substantial differences, with 19 volatile compounds identified as differentially present between the two decoctions. Despite these compositional differences, both decoction types demonstrated equivalent antithrombotic efficacy. Overall, the quality of DGD from manufacturers A and B aligned closely with that of TD. However, TD exhibited a higher overall quality compared to DGD, while both decoctions maintained consistent antithrombotic efficacy.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting the Antibody Fab Region Using Light-Induced Indole-3-Butyric Acid Functionalized Magnetic Microspheres","authors":"Yu Yi,&nbsp;Yao Li,&nbsp;Sa Wang,&nbsp;Yuting Liang,&nbsp;Jianfeng Mei,&nbsp;Guoqing Ying","doi":"10.1002/jssc.70086","DOIUrl":"https://doi.org/10.1002/jssc.70086","url":null,"abstract":"<div>\u0000 \u0000 <p>A novel light-controlled adsorption system for direct targeting of antibody Fab fragments was developed by utilizing indole-3-butyric acid functionalized magnetic microspheres. Indole-3-butyric acid, serving as a specific small molecule ligand, was successfully conjugated to amine-functionalized magnetic microspheres via a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/<i>N</i>-hydroxysuccinimide activation strategy. Under illumination at a particular wavelength, the indole-3-butyric acid ligand generated reactive radicals that interacted with the nucleotide-binding sites of antibody Fab fragments, enabling effective affinity adsorption. Static adsorption experiments demonstrated that the system's adsorption behavior obeys the Langmuir model (<i>K</i>_F = 0.122, <i>R</i>² = 0.996), indicating a homogeneous adsorption process. Kinetic studies further revealed that the adsorption process follows a second-order kinetic model (<i>k</i>₂ = 0.0257, <i>R</i>² = 0.989). When compared with conventional antibody adsorption systems, this new system exhibited specific targeting of Fab fragments, enhanced selectivity, and adjustable properties. In particular, at pH 7.0, effective elution was achieved by increasing the salt concentration, with the eluted product retaining antigen-binding activity. The purification recovery rate exceeded 98%, and the system maintained effective adsorption and elution of Fab fragments across various pH conditions. Besides, even after 10 reuse cycles, the system retained more than 96% of its efficiency, thus presenting excellent regenerability and reusability. In summary, the developed light-controlled antibody Fab region adsorption system offers a highly efficient, stable, and cost-effective approach. It is also expected to become one of the most effective methods for antibody Fab purification in the future.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Consistency Evaluation of Chemical Composition and Pharmacology of Pheretima aspergillum Dispensing Granules and Traditional Decoction Based on HPLC, GC-IMS, and Animal Experiments","authors":"Han Li,&nbsp;Xiaoming Wu,&nbsp;Yan Miao,&nbsp;Fengyu Dong,&nbsp;Xinjing Gui,&nbsp;Panpan Wang,&nbsp;Xuelin Li,&nbsp;Ruixin Liu","doi":"10.1002/jssc.70090","DOIUrl":"https://doi.org/10.1002/jssc.70090","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigates the consistency of quality between the traditional decoction (TD) of <i>Pheretima aspergillum</i> and its dispensing granule decoction (DGD) by examining their chemical composition and antithrombotic efficacy. We first determined the nucleoside components, protein content, and volatile compounds in 10 batches of TD and 9 batches of DGD sourced from three manufacturers using high-performance liquid chromatography (HPLC), UV–vis spectrophotometry, and gas chromatography ion mobility spectrometry (GC-IMS). Next, we assessed antithrombotic efficacy by measuring the relative length of tail thrombosis and calculating the thrombus inhibition rates at 24 and 48 h. Our comprehensive analysis revealed that the concentrations of hypoxanthine, uridine, inosine, adenosine, and proteins in TD were significantly higher than those in DGD. Moreover, the types and concentrations of volatile compounds in TD and DGD exhibited substantial differences, with 19 volatile compounds identified as differentially present between the two decoctions. Despite these compositional differences, both decoction types demonstrated equivalent antithrombotic efficacy. Overall, the quality of DGD from manufacturers A and B aligned closely with that of TD. However, TD exhibited a higher overall quality compared to DGD, while both decoctions maintained consistent antithrombotic efficacy.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation and Purification of Ginsenosides and Flavonoids in From the Leaves and Stems of Panax quinquefolium by High-Speed Countercurrent Chromatography and Online-Storage Inner-Recycling Countercurrent Chromatography","authors":"Chuangchuang Wang,&nbsp;Jinqian Yu,&nbsp;Yingjian Guo,&nbsp;Min Jiang,&nbsp;Kai Zhong,&nbsp;Xiao Wang","doi":"10.1002/jssc.70073","DOIUrl":"https://doi.org/10.1002/jssc.70073","url":null,"abstract":"<div>\u0000 \u0000 <p>Study aimed to isolate and purify compounds from the stems and leaves of <i>Panax quinquefolius</i>. By employing a highly innovative separation technique that combined multistage countercurrent chromatography (MRCC), high-speed countercurrent chromatography (HSCCC), and an advanced online-storage inner-recycling countercurrent chromatography (OS-IRCCC) mode for the first time, 12 compounds were successfully isolated, including 10 ginsenosides and 2 flavonoids. First, the crude extract was fractionated into five parts using D101 MRCC, with HPLC analysis revealing that 40% and 60% ethanol eluate contained the highest compound diversity. Overall, 40% ethanol eluate was separated using the solvent system of EtOAc/<i>n</i>-BuOH/H₂O (2:1:3, v/v), whereas 60% ethanol eluate underwent traditional countercurrent chromatography coupled with OS-IRCCC separation using the solvent system of methyl tert-butyl ether (MTBE)/<i>n</i>-BuOH/ACN/H₂O (4:2:3:8, v/v). Ultimately, various compounds were obtained, including kaempferol 3-<i>O</i>-<i>β</i>-<span>d</span>-glucopyranosyl-(1 → 2)-<i>β</i>-<span>d</span>-galactopyranosyl-7-<i>O</i>-<i>α</i>-<span>l</span>-rhamnopyranoside (13.2 mg), ginsenoside Rc (7.4 mg), 20(R)-ginsenoside Rh₁ (7.2 mg), ginsenoside Re (12.3 mg), kaempferol 3-<i>O</i>-<i>β</i>-<span>d</span>-glucopyranosyl-(1 → 2)-<i>β</i>-<span>d</span>-galactopyranoside (14.1 mg), ginsenoside Rb₁ (8.2 mg), ginsenoside Rb₂ (17.5 mg), ginsenoside Rb₃ (27.3 mg), ginsenoside Rg₁ (13.3 mg), ginsenoside Rg₂ (9.7 mg), ginsenoside Rd (11.4 mg), and pseudo-ginsenoside F₁₁ (16.7 mg). This research highlights the efficacy of the novel separation technique in isolating and purifying valuable compounds from <i>P. quinquefolius</i> stems and leaves.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocol for Oligonucleotides Characterization Using Hydrophilic Interaction Chromatography","authors":"Davy Guillarme,&nbsp;Szabolcs Fekete,&nbsp;Sylwia Studzińska","doi":"10.1002/jssc.70088","DOIUrl":"10.1002/jssc.70088","url":null,"abstract":"<div>\u0000 \u0000 <p>Oligonucleotides (ONs) are an increasingly popular category of molecules in the pharmaceutical landscape, particularly attractive for the treatment of genetic and rare diseases. However, analyzing these molecules presents significant challenges, due to their highly hydrophilic nature, multiple negative charges, and the presence of closely related impurities resulting from the complex solid-phase synthesis process. Ion pairing reverse-phase liquid chromatography (IP-RPLC) is the preferred technique for ONs analysis but is not ideal for mass spectrometry (MS) coupling. Consequently, there is a growing interest in exploring alternative strategies with hydrophilic interaction chromatography (HILIC) emerging as one of the most promising options. As HILIC is not yet fully established for the analysis of ONs, we have prepared this protocol paper to facilitate entry into this field. It not only provides best practices, opportunities, and potential advantages but also caveats and other important considerations for using HILIC to characterize ONs. The paper addresses the selection of stationary and mobile phases, optimization of gradient conditions, MS coupling, and key aspects to consider when manipulating ON samples. We hope this protocol will help establish HILIC as a more universal solution for ONs analysis.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Synthesis of Polyaniline@Nano Zinc Oxide/Modified Multiwalled Carbon Nanotubes Nanocomposite Coating for Solid Phase Microextraction of Some Polycyclic Aromatic Hydrocarbons in Coffee and Grilled Meat","authors":"Rezvan Askari Baduee,&nbsp;Maryam Kazemipour,&nbsp;Mehdi Ansari,&nbsp;Mohammad Mehdipour,&nbsp;Neda Mohamadi","doi":"10.1002/jssc.70080","DOIUrl":"10.1002/jssc.70080","url":null,"abstract":"<div>\u0000 \u0000 <p>Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic compounds resulting from incomplete burning of organic materials. This work describes the successful layer-by-layer fabrication of a novel zinc oxide nanocomposite made of zinc oxide nanoparticles, aniline, sodium dodecyl sulfate, and modified multi-walled carbon nanotubes on a stainless steel wire by electrodeposition. The coating and extraction conditions were screened, optimized, and validated using factorial design and central composite design, respectively. The prepared nanocomposites were characterized by the Fourier-transform infrared spectroscopy, field-emission scanning electron microscopy, X-ray diffraction analysis, and Brunauer-Emmett-Teller techniques. It featured a porous structure, excellent heat stability, and a high extraction capacity. It also adhered well to the steel surface. Extracting PAHs was optimized through experimental design. The optimal conditions obtained for extraction were 60 min, 30%, and 30°C for extraction time, amount of salt, and extraction temperature, respectively. The validated method generally showed a linear range between 0.01 to 1.50 µg/mL with a linearity of (R²) of 0.9902–0.9992, limits of detection less than 0.003 µg/mL, limit of quantification lower than 0.010 µg/mL, and relative standard deviation percent less than 45. Recovery values of the analytes in food samples varied between 53.4% and 110.1%. The proposed method was employed in the extraction and determination of some PAHs in some food samples while naphthalene, anthracene, phenanthrene, benzo[a]pyrene, and benz[a]anthracene were detected in certain food samples. Overall, our research suggests a novel nanocomposite as a potential fiber coating that enables high-capacity PAH extraction.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyrin-Based Covalent Organic Framework Reinforced Hollow Fiber for Solid-Phase Microextraction of Tebuconazole and Propiconazole","authors":"Marzieh Kavian,&nbsp;Milad Ghani,&nbsp;Jahan Bakhsh Raoof","doi":"10.1002/jssc.70077","DOIUrl":"10.1002/jssc.70077","url":null,"abstract":"<div>\u0000 \u0000 <p>Herein, an amino-functionalized covalent organic framework was synthesized and accommodated in the pores of porous hollow fiber. In this context, tetra (4-aminophenyl) porphyrin was synthesized for preparing the desired covalent organic framework as the extracting sorbent and employed for hollow fiber solid-phase microextraction of tebuconazole and propiconazole. With respect to the amino groups of the as-synthesized porphyrin-based covalent organic framework, the extracting device has the ability of establishing a hydrogen bond with the selected model analytes. Under the optimum condition, the linear range of the method for both analytes were estimated in the range of 0.5–200 µg L⁻¹ (the coefficient of determination of 0.9962 for tebuconazole and 0.9990 for propiconazole). The limits of detection of the method for tebuconazole and propiconazole were calculated to 0.02 µg g⁻¹ and 0.03 µg g⁻¹, respectively. The limits of quantification of the method were also estimated for two analytes equal to 0.07 and 0.08 µg g⁻¹, respectively. The intra- and inter-day relative standard deviations, which fell between 1.8% and 4.7%, were computed to assess the accuracy of the suggested approach. The proposed method was used for the extraction and determination of tebuconazole and propiconazole in tomato, cucumber, apple, cabbage, and carrot, which the obtained results showed the success of the method in extracting and determining of these analytes from the target samples.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Polyaniline Modified Cellulose Filter Paper and Its Application in Detecting 23 Per- and Polyfluoroalkyl Substances","authors":"Hong-Yu You,&nbsp;Hong-Yi Yin,&nbsp;Jia-Hui Zhao,&nbsp;Zhou-Yang Xiang,&nbsp;Bo Liu,&nbsp;Guo-Wei Hu,&nbsp;Zhong-Hui Sun,&nbsp;Hua-Jie Lai","doi":"10.1002/jssc.70076","DOIUrl":"10.1002/jssc.70076","url":null,"abstract":"<div>\u0000 \u0000 <p>Per- and polyfluoroalkyl substances (PFAS) are a widely used class of synthetic chemicals that pose a significant global environmental and health threat due to their persistent and bioaccumulation toxicity caused by strong C-F bonds in their structures. PFAS usually exist in trace concentrations in environmental water bodies, which poses great challenges for environmental analysis. In this study, environmentally friendly cellulose was modified with polyaniline through in situ oxidative polymerization, and used as the filter paper for solid-phase extracting 23 PFAS in water. Characterization techniques such as scanning electron microscope, Fourier transform infrared spectroscopy, X-ray photoelectron spectrometer, and thermogravimetric analysis indicated the successful synthesis of structurally stable polyaniline-modified cellulose filter paper (PANI/CFP). Then, a high-performance liquid chromatograph-triple quadrupole mass spectrometry determination method was established for 23 PFAS from water using PANI/CFP extraction. The optimal adsorption occurred at a solution pH of 3.0–5.0, and the best elution efficiency can be achieved using methanol with 2% added ammonia water. The adsorption mechanism of 23 PFAS by PANI/CFP can be considered as the result of synergistic effects between physical and chemical adsorption. The recovery rates for 23 PFAS in groundwater, surface water, and wastewater ranged from 65.8% to 105%, with limits of detection and quantification from 0.44 to 36.5 ng/L. After being reused five times, PANI/CFP can still maintain a good extraction recovery rate for PFAS. This study successfully prepared PANI/CFP and established an experimental method flow for its application in the extraction and detection of 23 PFAS in water. The PANI/CFP has the characteristics of stable structure and properties, and easy operation.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}