Journal of separation science最新文献

{"title":"Rapid Purification Strategies for Monoclonal Antibodies: A Case Study","authors":"Qian Li,&nbsp;Xiaoying Liang,&nbsp;Hongyang Zhao,&nbsp;Qingquan He,&nbsp;Guozhu Li,&nbsp;Zicheng Wang,&nbsp;Guohong Qin,&nbsp;Dan Xu","doi":"10.1002/jssc.70232","DOIUrl":"10.1002/jssc.70232","url":null,"abstract":"<div>\u0000 \u0000 <p>Monoclonal antibodies (mAbs) are widely used in drug development for their precise targeting and lower adverse effects. Market competition drives demand for more efficient mAb production processes. We developed a mAbs purification platform integrating protein A membrane chromatography with sequential anion/cation exchange. Compared with conventional protein A column chromatography (HiTrap MabSelect PrismA), protein A membrane chromatography (HiTrap Fibro PrismA) achieved a 96% reduction in process time (7.5 vs. 190 min) with similar product quality. A tandem system (Eshmuno CP-FT + Natrix Q Micro) showed robust performance at high loads (up to 1000 g/L). Specifically, this system reduced high molecular weight impurities from 1.9% to 0.2% and host cell protein levels from 630.4 to 19.77 ppm, while maintaining a recovery yield exceeding 90%. Combined rapid affinity membrane and high-capacity tandem exchange steps reduced medium costs by 11.8%. This platform demonstrates high efficiency for mAbs purification workflows.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Stable Cinchona Chiral Stationary Phase Based on Mercaptopropylsilane Modified Polydivinylbenzene Microspheres","authors":"Mingming Cao,&nbsp;Changyu Cai,&nbsp;Yang Yang,&nbsp;Yanxiong Ke","doi":"10.1002/jssc.70236","DOIUrl":"10.1002/jssc.70236","url":null,"abstract":"<div>\u0000 \u0000 <p>In this work, the preparation of a low-swelling mercaptopropyltrimethoxysilane (MPTMS)-modified polydivinylbenzene micropheres matrix for cinchona chiral stationary phases is reported. It was found that MPTMS preferentially deposits within the smaller pores of PDVB particles during the sol–gel process, leading to an increased average pore size and a narrower pore size distribution. The swelling resistance of the particles increased in proportion to the amount of MPTMS that was loaded. The measured swelling propensity (SP) value for PDVB-5SH-QN in methanol and acetonitrile are 0.097 and 0.226, respectively, which are considerably lower than the values of 0.759 and 1.142 obtained for polydivinylbenzene microspheres. The performance of the chiral column was evaluated using a sample set containing 20 FMOC-amino acids. The results indicated that the modification of organosilane led to enhancements in column efficiency. The mass transfer resistance coefficient in the Van Deemter equation for PDVB-5SH-QN (<i>C</i> = 1.86 µs; standard error = 0.11) is comparable to that for SiO₂-QN (<i>C</i> = 2.74 µs; standard error = 0.13). The enantioselectivity exhibited by the column is analogous to that observed in SiO₂-QN. Under alkaline mobile phase conditions, the polymer-based stationary exhibited excellent stability and reproducibility.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Enhanced Separation Techniques for Oligonucleotides Utilizing Mixed-Mode Chromatography and 2D-LC/UV/MS Analysis","authors":"Jakob Haglöf,&nbsp;Jenny M. Nilsson,&nbsp;Jufang Wu Ludvigsson","doi":"10.1002/jssc.70238","DOIUrl":"10.1002/jssc.70238","url":null,"abstract":"<p>Oligonucleotides are traditionally analyzed using ion-pair reversed-phase liquid chromatography. In this project, we explored an alternative approach utilizing a reversed phase-weak anion exchange mixed-mode column, which combines separation based on both charge and hydrophobicity. Our findings indicate that the mixed-mode column provided stronger retention and superior separation compared to the C18 column, eliminating the need for ion-pairing reagents. Additionally, selectivity can be adjusted using gradient pH and buffer concentration as well as acetonitrile content. However, the use of a phosphate buffer was necessary to ensure adequate retention and separation, despite its incompatibility with MS detection. To address this issue, a 2DLC-UV-qTOF setup with multiple loops was established, where the second-dimension LC, connected to the MS detector, required only an MS-compatible mobile phase. Using a HILIC-type LUNA Omega Sugar column in the second-dimension LC, the MS intensity of the heart-cut from the first dimension was increased 100-fold, allowing for the characterization of the main peak.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70238","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Difference Analysis of Raw and Salted-Steamed Products of Morinda officinalis Based on Color and Component Correlation","authors":"Yuan Zhang,&nbsp;Kexu Dong,&nbsp;Guoshun Shan,&nbsp;Jing Lian,&nbsp;Ji Shi,&nbsp;Li Jiang,&nbsp;Tianzhu Jia","doi":"10.1002/jssc.70239","DOIUrl":"10.1002/jssc.70239","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Morinda officinalis</i> (MO) is a commonly used traditional Chinese medicine for tonifying kidney yang and contains a large number of oligosaccharide components. Its salt-steamed products are widely used in clinical practice. However, the current standards are not very clear in distinguishing between the raw and processed products in terms of appearance and quality. In this study, ultra-high performance liquid chromatography (UHPLC) combined with mass spectrometry (MS) was used to qualitatively and quantitatively analyze MO and salt-steamed MO (SSMO). Three differential components were screened out by chemometrics, and the variation patterns of oligosaccharides during the steaming process were clarified. A colorimeter was used to evaluate the overall color of MO during the salt-steaming process. The correlation analysis indicated a notable connection between color metrics and chemical components. According to the results of this study, color values can be used as an effective tool to predict the content of components in raw and salt-processed MO. In conclusion, this research offers novel concepts and approaches for the identification and quality assessment of MO processed products.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measuring Solvation Interactions of Deep Eutectic Solvents Formed by Metal Chlorides and an Imidazolium Salt by Inverse Gas Chromatography","authors":"Siyuan Liu,&nbsp;Jared L. Anderson","doi":"10.1002/jssc.70231","DOIUrl":"10.1002/jssc.70231","url":null,"abstract":"<p>Deep eutectic solvents (DESs) represent a class of solvents that offer a number of advantages including minimal toxicity, affordability, low vapor pressure, and simple, environmentally friendly preparation methods. They have found utility in areas, such as gas absorption, metal plating, and extractions. However, the relationship between their solvation properties and chemical composition remains poorly understood. In this study, a broad range of Type I DESs composed of metal chlorides and an imidazolium salt were prepared, employed as gas chromatographic stationary phases, and characterized using the Abraham solvation parameter model by inverse gas chromatography. The Abraham solvation parameter model allows for the study of DES solvation properties and the effects of varying structural components on system constants using a linear-free energy relationship. The DESs were investigated by systematically varying their composition, including the type of metal chloride and the molar ratio between the metal chloride and the imidazolium salt. The results show that hydrogen bond acidity, hydrogen bond basicity, and dipolarity/polarizability interactions are strongly influenced by the type of metal chloride within the DES and the ratio of metal chloride to imidazolium salt in the eutectic mixture. Furthermore, a column pretreatment procedure is presented that enables the effective coating of highly polar DESs onto open tubular capillary gas chromatography columns.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Difference Analysis of Raw and Salted-Steamed Products of Morinda officinalis Based on Color and Component Correlation","authors":"Yuan Zhang,&nbsp;Kexu Dong,&nbsp;Guoshun Shan,&nbsp;Jing Lian,&nbsp;Ji Shi,&nbsp;Li Jiang,&nbsp;Tianzhu Jia","doi":"10.1002/jssc.70239","DOIUrl":"10.1002/jssc.70239","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Morinda officinalis</i> (MO) is a commonly used traditional Chinese medicine for tonifying kidney yang and contains a large number of oligosaccharide components. Its salt-steamed products are widely used in clinical practice. However, the current standards are not very clear in distinguishing between the raw and processed products in terms of appearance and quality. In this study, ultra-high performance liquid chromatography (UHPLC) combined with mass spectrometry (MS) was used to qualitatively and quantitatively analyze MO and salt-steamed MO (SSMO). Three differential components were screened out by chemometrics, and the variation patterns of oligosaccharides during the steaming process were clarified. A colorimeter was used to evaluate the overall color of MO during the salt-steaming process. The correlation analysis indicated a notable connection between color metrics and chemical components. According to the results of this study, color values can be used as an effective tool to predict the content of components in raw and salt-processed MO. In conclusion, this research offers novel concepts and approaches for the identification and quality assessment of MO processed products.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chain Length-Dependent Reverse Micelle Extraction of Prolamins From Baijiu Jiuzao: Synergistic Effects of Ethanol, Ionic Strength, and Chaotropic Agent","authors":"TingTing Yu,&nbsp;FuRong Yang,&nbsp;Fang Wang,&nbsp;YongMei Chen,&nbsp;Yi Liu,&nbsp;Nan Hu","doi":"10.1002/jssc.70242","DOIUrl":"10.1002/jssc.70242","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigated the chain length-dependent extraction efficiency of two cationic surfactants—dodecyltrimethylammonium bromide (C12, 12-carbon chain) and cetyltrimethylammonium bromide (C16, 16-carbon chain)—for reverse micelle extraction of prolamins from Baijiu Jiuzao (Chinese Baijiu distillers' grains). Although pH variations (4.0–9.0) had negligible effects, ethanol, ionic strength, and chaotropic agent demonstrated distinct chain length-dependent behaviors: ethanol increased C12's forward extraction efficiency by 10.6% (absolute) while enhancing C16's backward efficiency by 8.7%. Potassium chloride enhanced C16's forward efficiency by 4.7%, while potassium bromide significantly improved C12's backward efficiency by 15.9%. Guanidine hydrochloride consistently promoted C16 in both extraction phases. Dynamic light scattering demonstrated significant differences (<i>p</i> &lt; 0.05) in reverse micelle sizes: C12 formed smaller empty reverse micelles (1.5 ± 0.2 nm) compared to C16 (2.5 ± 0.5 nm). During forward extraction, reverse micelles expanded significantly to 217.9 ± 6.3 nm (C12) and 311.2 ± 43.2 nm (C16). Backward extraction released prolamin particles of 449.0 ± 16.2 nm (C12) and 650.4 ± 29.7 nm (C16), confirming the size-exclusion-driven release mechanism. The more compact C12 reverse micelles correlated with lower total prolamin yield (26.6 ± 0.1% vs. 32.2 ± 0.1% for C16, <i>p</i> &lt; 0.01) and substantially diminished bovine serum albumin recovery (5.2 ± 0.2% vs. 45.3 ± 2.5%, <i>p</i> &lt; 0.01). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis corroborated these findings, further demonstrating that reverse micelle-extracted prolamins formed thermally stable dimers resistant to reductive cleavage. Combined with dynamic light scattering data (particle size range: 400–700 nm in the backward-extraction aqueous phase), these findings demonstrate that prolamin aggregates after reverse micelle extraction. These findings demonstrate that surfactant chain length as a key parameter for optimizing reverse micellar systems, where shorter-chain surfactants facilitate selective extraction, whereas longer chains enhance yields.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chain Length-Dependent Reverse Micelle Extraction of Prolamins From Baijiu Jiuzao: Synergistic Effects of Ethanol, Ionic Strength, and Chaotropic Agent","authors":"TingTing Yu,&nbsp;FuRong Yang,&nbsp;Fang Wang,&nbsp;YongMei Chen,&nbsp;Yi Liu,&nbsp;Nan Hu","doi":"10.1002/jssc.70242","DOIUrl":"10.1002/jssc.70242","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigated the chain length-dependent extraction efficiency of two cationic surfactants—dodecyltrimethylammonium bromide (C12, 12-carbon chain) and cetyltrimethylammonium bromide (C16, 16-carbon chain)—for reverse micelle extraction of prolamins from Baijiu Jiuzao (Chinese Baijiu distillers' grains). Although pH variations (4.0–9.0) had negligible effects, ethanol, ionic strength, and chaotropic agent demonstrated distinct chain length-dependent behaviors: ethanol increased C12's forward extraction efficiency by 10.6% (absolute) while enhancing C16's backward efficiency by 8.7%. Potassium chloride enhanced C16's forward efficiency by 4.7%, while potassium bromide significantly improved C12's backward efficiency by 15.9%. Guanidine hydrochloride consistently promoted C16 in both extraction phases. Dynamic light scattering demonstrated significant differences (<i>p</i> &lt; 0.05) in reverse micelle sizes: C12 formed smaller empty reverse micelles (1.5 ± 0.2 nm) compared to C16 (2.5 ± 0.5 nm). During forward extraction, reverse micelles expanded significantly to 217.9 ± 6.3 nm (C12) and 311.2 ± 43.2 nm (C16). Backward extraction released prolamin particles of 449.0 ± 16.2 nm (C12) and 650.4 ± 29.7 nm (C16), confirming the size-exclusion-driven release mechanism. The more compact C12 reverse micelles correlated with lower total prolamin yield (26.6 ± 0.1% vs. 32.2 ± 0.1% for C16, <i>p</i> &lt; 0.01) and substantially diminished bovine serum albumin recovery (5.2 ± 0.2% vs. 45.3 ± 2.5%, <i>p</i> &lt; 0.01). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis corroborated these findings, further demonstrating that reverse micelle-extracted prolamins formed thermally stable dimers resistant to reductive cleavage. Combined with dynamic light scattering data (particle size range: 400–700 nm in the backward-extraction aqueous phase), these findings demonstrate that prolamin aggregates after reverse micelle extraction. These findings demonstrate that surfactant chain length as a key parameter for optimizing reverse micellar systems, where shorter-chain surfactants facilitate selective extraction, whereas longer chains enhance yields.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measuring Solvation Interactions of Deep Eutectic Solvents Formed by Metal Chlorides and an Imidazolium Salt by Inverse Gas Chromatography","authors":"Siyuan Liu,&nbsp;Jared L. Anderson","doi":"10.1002/jssc.70231","DOIUrl":"10.1002/jssc.70231","url":null,"abstract":"<p>Deep eutectic solvents (DESs) represent a class of solvents that offer a number of advantages including minimal toxicity, affordability, low vapor pressure, and simple, environmentally friendly preparation methods. They have found utility in areas, such as gas absorption, metal plating, and extractions. However, the relationship between their solvation properties and chemical composition remains poorly understood. In this study, a broad range of Type I DESs composed of metal chlorides and an imidazolium salt were prepared, employed as gas chromatographic stationary phases, and characterized using the Abraham solvation parameter model by inverse gas chromatography. The Abraham solvation parameter model allows for the study of DES solvation properties and the effects of varying structural components on system constants using a linear-free energy relationship. The DESs were investigated by systematically varying their composition, including the type of metal chloride and the molar ratio between the metal chloride and the imidazolium salt. The results show that hydrogen bond acidity, hydrogen bond basicity, and dipolarity/polarizability interactions are strongly influenced by the type of metal chloride within the DES and the ratio of metal chloride to imidazolium salt in the eutectic mixture. Furthermore, a column pretreatment procedure is presented that enables the effective coating of highly polar DESs onto open tubular capillary gas chromatography columns.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Last 10 Years of Research in the Enantioseparation of Pharmacologically Relevant Phytocannabinoids: An Updated Review","authors":"Ina Varfaj,&nbsp;Federica Ianni,&nbsp;Ghaid W. A. Abualzulof,&nbsp;Anna Migni,&nbsp;Laura Mercolini,&nbsp;Sandra Furlanetto,&nbsp;Andrea Carotti,&nbsp;Roccaldo Sardella","doi":"10.1002/jssc.70234","DOIUrl":"10.1002/jssc.70234","url":null,"abstract":"<div>\u0000 \u0000 <p>As research on <i>Cannabis sativa</i> L. and its derivatives expands, an increasing number of novel phytocannabinoids are being identified. Many of these compounds exhibit molecular chirality due to the presence of one or more stereogenic centers in their structures. Given the pharmacological and toxicological relevance of chiral molecules, regulatory authorities are expected to demand more stringent analytical characterization of the enantiomeric composition of phytocannabinoids in <i>C. sativa</i> L. extracts. The growing availability and enhanced performance of advanced analytical technologies, particularly chiral chromatographic systems, have renewed interest in the stereochemical profiling of these natural products. Chiral separation techniques have become central to research efforts aimed at determining enantiomeric purity and understanding stereoisomeric distributions in complex plant matrices. Among the limited studies addressing chiral chromatographic methods for separating phytocannabinoid enantiomers and diastereomers, two techniques are predominant: high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC). Method development in both approaches typically relies on two major classes of chiral stationary phases (CSPs). The first comprises Pirkle-type CSPs, which utilize the “Inverted Chirality Columns Approach” (ICCA) and are based on rational design principles for enantioselective interactions. The second group includes polysaccharide-based CSPs employing cellulose or amylose derivatives, recognized for their broad applicability in resolving diverse phytocannabinoid enantiomers. This work will review a selection of recent, representative studies, highlighting key analytical strategies for chiral separation, methodological challenges, and implications for both scientific research and regulatory frameworks.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}