Journal of separation science最新文献

{"title":"Development and Validation of AAV Capsids Separation on Specimen Columns for Reproducibility Evaluation of Large-Scale Chromatographic Monoliths","authors":"Rok Miklavčič,&nbsp;Tina Simčič,&nbsp;Sara Rotar,&nbsp;Polona Komel,&nbsp;Rok Žigon,&nbsp;Dona Pavlovič,&nbsp;Ines Bergoč,&nbsp;Domen Ipavec,&nbsp;Ana Simčič Zuljan,&nbsp;Ažbe Žnidaršič,&nbsp;Dolores Kukanja,&nbsp;Jana Vidič,&nbsp;Aleš Štrancar,&nbsp;Urh Černigoj","doi":"10.1002/jssc.70114","DOIUrl":"https://doi.org/10.1002/jssc.70114","url":null,"abstract":"<p>One of the key challenges in adeno-associated virus (AAV) viral vector manufacturing is the effective and consistent separation of full (F) AAV capsids from undesired non-functional (empty = E, partially filled, etc.) capsids. Typically, at least one chromatography step is used for this purpose in AAV manufacturing. Due to the complexity of viral capsids separation, even a small change in the chromatographic process is reflected in unreproducible results. One solution for robust polishing of full AAV capsids is the highly reproducible (HR) design of chromatographic columns used in this step. Implementation of such columns requires the development of control tests, which efficiently predict column performance for AAV separation. In this paper, the methodology for reproducible separation of empty and full recombinant AAV2/8 (E/F rAAV2/8) capsids was defined using quaternary amine (QA) chromatographic monoliths in a linear potassium chloride (KCl) gradient. The scalability of the procedure was experimentally confirmed on 1, 80, and 800 mL CIMmultus QA columns, where empty capsids eluted at a KCl concentration range of 89.4–91.4 mM. A sampling of the monolith material from the 800 mL CIMmultus QA column and testing it for E/F rAAV2/8 capsid separation in the form of a 200 µL column resulted in a highly comparable elution pattern as obtained with the parent 800 mL column. The principle of sampling material by cutting the parent monolith, packing it in 200 µL columns (specimens) and testing them for E/F rAAV2/8 capsid separation was further developed to demonstrate intra-column homogeneity; batch-to-batch homogeneity; and scalability of CIM QA monoliths. Finally, specimens testing using a validated E/F rAAV2/8 separation method was used to monitor 28 CIMmultus QA production batches (bed volumes between 1 and 8000 mL). E rAAV2/8 capsids eluted at KCl concentration between 89.3 and 95.3 mM for 28 batches, paving the way for commercialization of highly reproducible preparative QA chromatographic monoliths (CIMmultus QA HR).</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic Solid Phase Extraction of Lead (II) and Cadmium (II) From Water Samples Using ZnO@Fe3O4 Nanoparticles Combined With Flame Atomic Absorption Spectrometry Determination","authors":"Şeyma Korkmaz,&nbsp;Elvan Hasanoğlu Özkan,&nbsp;Demet Uzun,&nbsp;Nurdan Kurnaz Yetim,&nbsp;Cemile Özcan","doi":"10.1002/jssc.70115","DOIUrl":"https://doi.org/10.1002/jssc.70115","url":null,"abstract":"<p>A new magnetic solid phase extraction method was developed utilizing magnetically enhanced zinc oxide (ZnO@Fe₃O₄) nanoparticles for the separation and enrichment of lead (II) and cadmium (II) before sample determination through flame atomic absorption spectrometry. The ZnO@Fe₃O₄ nanoparticles synthesized by the hydrothermal method were characterized by x-ray diffraction, scanning electron microscopy, and energy-dispersive x-ray spectroscopy. The experimental factors like pH (6.0), eluent type and concentration (1 M nitric acid for lead (II), 1 M hydrochloric acid for cadmium (II)), adsorbent amount (150 mg for lead (II), and 200 mg for cadmium (II)), sample volume (5 mL), and extraction time (5 min for lead (II), and 10 min for cadmium (II)) were optimized. Under the optimization conditions, limits of detection of 2.36 and 7.86 µg/L for lead (II) and cadmium (II) were obtained with a wide concentration range from 50 to 5000 µg/L, respectively. The presented method was successfully applied to real water samples with spiked recoveries in the range of 99.2%–101% for lead (II) and 99.6%–100% for cadmium (II).</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Hybrid Molecularly Imprinted Polymers Based on 3-Triethoxysilylpropyl Methacrylic Amide for Solid-Phase Extraction of Gatifloxacin From Lake Water","authors":"Meihong Chai,&nbsp;Chang Liu,&nbsp;Liping Zhang,&nbsp;Yi Wang,&nbsp;Xueyan Zhen,&nbsp;Yi Yang,&nbsp;Yanping Huang,&nbsp;Zhaosheng Liu","doi":"10.1002/jssc.70122","DOIUrl":"https://doi.org/10.1002/jssc.70122","url":null,"abstract":"<div>\u0000 \u0000 <p>A novel molecularly imprinted polymer (MIP) against gatifloxacin based on a hybrid monomer (3-triethoxysilylpropyl methacrylic amide, APTES-MAA) was reported. In this study, the imprinted monolith was synthesized with gatifloxacin as template, ethylene glycol dimethacrylate (EGDMA) as cross-linker, and APTES-MAA as functional monomer in a mixture of acetonitrile and isooctane as porogen via an optimization based on density functional theory (DFT). The polymers were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and mercury porosimetry. The resulting hybrid MIPs (HMIPs) showed high affinity and selectivity to the template molecule with an imprinted factor of 16.28 ± 0.53, which was 6.1 times higher than that of the traditional methacrylic acid–based MIP. The MIP was used as an adsorbent in solid-phase extraction of lake water with gatifloxacin. The recovery was 96.52 ± 2.69%. The method exhibits the advantageous features of cost-effectiveness and high sensitivity, rendering it a promising approach for analyzing organic pollutants in water.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Chemical Composition of Long-Chain Linear Alkylbenzenes Using High-Performance Liquid Chromatography Coupled with Microwave Plasma Torch Mass Spectrometry","authors":"Yuwen He,&nbsp;Jing Luo,&nbsp;Pengcheng Hu,&nbsp;Xingmei Song,&nbsp;Min Zhao,&nbsp;Guozhou Wang,&nbsp;Fengjian Chu,&nbsp;Hongru Feng,&nbsp;Yuanjiang Pan","doi":"10.1002/jssc.70123","DOIUrl":"https://doi.org/10.1002/jssc.70123","url":null,"abstract":"<div>\u0000 \u0000 <p>Long-chain linear alkylbenzenes (LABs) are essential precursors to produce alkylbenzene sulfonates, which have high surfactant properties and are readily biodegradable, widely used in various detergents. However, an effective and rapid method for the direct analysis and quality control of LAB chemical composition still needs to be improved. This study uses high-performance liquid chromatography coupled with microwave plasma torch mass spectrometry (HPLC-MPT-MS) to analyze the composition of long-chain LABs and quantify nine of these components. The results show that the main components in long-chain LAB samples are eicosylbenzene, docosylbenzene, and tetracosylbenzene, primarily including four structures, three of which have been structurally identified. This study successfully separates and analyzes LAB directly via HPLC-MPT-MS, providing a valuable and reliable method for the quality evaluation of LAB.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides","authors":"Filip Dusa,&nbsp;Marcelina Rusin,&nbsp;Denisa Smolkova,&nbsp;Jozef Sestak,&nbsp;Justyna Dobrowolska-Iwanek,&nbsp;Michał Woźniakiewicz,&nbsp;Jana Lavicka","doi":"10.1002/jssc.70112","DOIUrl":"https://doi.org/10.1002/jssc.70112","url":null,"abstract":"<p>The high-sensitivity capabilities of laser-induced fluorescence (LIF) detection continuously promote the development of various labels with different fluorescence properties. However, this strategy also requires the adaptation of existing detection systems to suit the excitation and emission characteristics of novel fluorescent tags. In this study, we adapted the LIF detector of the commercial capillary electrophoresis instrument to the specific fluorescence spectra of 2-aminoacridone labeled human milk oligosaccharides. An external solid-state laser with a wavelength of 405 nm was connected to the commercial capillary electrophoresis instrument via a simple 3D-printed laser-to-light-guide adapter, and different optical filter setups were compared based on the signal-to-noise ratio. The optimized setup provided detection limits as low as 0.27 to 0.34 nM, corresponding to injection of 3.4 to 4.6 attomoles of 2-aminoacridone labeled oligosaccharides. These findings show that the optimized laser and filter configuration can enhance the sensitivity of electrophoretic separation by several orders of magnitude. In addition, the presented setup can be utilized as a guide for coupling different lasers to the commercial instrument.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic Solid-Phase Extraction of Benzoic Acids in Fruit Juices Using Hydrophilic Metal-Organic Framework MIL-101(Cr) Coated with Magnetic Nanoparticles","authors":"Yu Jiang,&nbsp;Hong Pan,&nbsp;Menghong Xie,&nbsp;Huifeng Deng,&nbsp;Guihua Ruan,&nbsp;Yipeng Huang","doi":"10.1002/jssc.70118","DOIUrl":"https://doi.org/10.1002/jssc.70118","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, magnetic Fe₃O₄-coated metal-organic framework composites, CONH₂-MIL-101(Cr)/Fe₃O₄, were synthesized by depositing Fe₃O₄ particles on the surface of amidated MIL-101(Cr). The scanning electron microscope images show that the MIL-101(Cr) crystals were uniformly coated by Fe₃O₄ particles. The magnetic hysteresis curve of CONH₂-MIL-101(Cr)/Fe₃O₄ reveals a saturated magnetization value of 23.47 emu/g. The water contact angle decreases from 61.00° to 12.88° after amidation and Fe₃O₄ coating. The magnetic solid-phase extraction (MSPE) efficiency of CONH₂-MIL-101(Cr)/Fe₃O₄ for benzoic acid derivatives was studied. Under optimal conditions, the adsorption and desorption processes can be completed within 5 min, and the maximum adsorption capacity to protocatechuic acid (PCA) and vanillic acid (VA) is 10.31 and 17.30 mg/g. Coupling the MSPE with high-performance liquid chromatography-ultraviolet detection, the detection limit for PCA and VA was 0.29 and 0.58 ng/mL, respectively. The mean recoveries of PCA and VA in spiked samples ranged from 82.85%–91.27% with relative standard deviations lower than 0.33%–5.33%. This study provides inspiration for the fabrication of novel magnetic sorbents, and a reliable method for the adsorption and analysis of benzoic acid derivatives.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-Site Quantification of Transformation Products of Rocket Fuel Unsymmetrical Dimethylhydrazine in Air Using Solid-Phase Microextraction","authors":"Bauyrzhan Bukenov,&nbsp;Nurbi Karimkyzy,&nbsp;Aruzhan Smail,&nbsp;Tolkyn Kurmanbayeva,&nbsp;Bulat Kenessov","doi":"10.1002/jssc.70113","DOIUrl":"https://doi.org/10.1002/jssc.70113","url":null,"abstract":"<div>\u0000 \u0000 <p>Despite the high importance of the monitoring of air pollution with transformation products (TPs) of rocket fuel unsymmetrical dimethylhydrazine (UDMH) in the regions of UDMH spills, there is a lack of simple, fast, and accurate methods for quantitation of UDMH TPs in air. This study was aimed at the development of the new method for on-site quantitation of UDMH TPs in air using solid-phase microextraction (SPME) and a portable gas chromatograph with nitrogen–phosphorus detector (GC-NPD). When using a known approach based on SPME from 20-mL vials filled with air, limits of detection (LODs) with a portable GC-NPD ranged from 4 to 10 µg/m³ that is about one order of magnitude higher than with a stationary GC–MS and can be insufficient for detecting UDMH TPs in real air samples. For achieving lower LODs, the sample volume has been increased to 250 mL. According to the modeling and experimental results, the optimal extraction time from 250-mL gas sampling bulbs was 15 min. Increase in the sample volume to 250 mL and extraction time to 15 min provided LODs 0.3–2 µg/m³. To enhance on-site measurements, preliminary screening using 24-h SPME from the liner (internal diameter 4.6 mm) exposed to the open air has been implemented. According to the modeling, such extraction parameters should provide 2.0–3.5 times greater amounts of analytes in the fiber coating. After screening, using this approach on the recent fall place of the first stage of the “Proton-M” carrier rocket, no analytes have been detected.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionic Liquid-Based Polarity-Adjustable Deep Eutectic Solvent Extraction Followed by High-Performance Liquid Chromatography-Diode-Array Detection for the Determination of Liposoluble Anthraquinones in Salvia miltiorrhiza Bge. Root","authors":"Jinghui Li,&nbsp;Yan Kang,&nbsp;Ying Wang,&nbsp;Jiaxin Liu,&nbsp;Yingting Wang,&nbsp;Sitong Liu,&nbsp;Yunxi Bu,&nbsp;Xiangqun Li,&nbsp;Jiahan Xie,&nbsp;Zhibing Wang","doi":"10.1002/jssc.70116","DOIUrl":"https://doi.org/10.1002/jssc.70116","url":null,"abstract":"<div>\u0000 \u0000 <p>A simple, green, and efficient ultrasound-assisted deep eutectic solvents (DESs) extraction followed by high-performance liquid chromatography-diode array detector was established to detect four liposoluble anthraquinones in <i>Salvia miltiorrhiza</i> Bge. root. In this study, a series of polarity-adjustable DESs were prepared using <i>n</i>-hexanol and tetrabutylammonium chloride, and their structure, density, viscosity, pH, and thermal stability were determined. The conditions for extraction were optimized through single-factor experiments and response surface methodology. The kinetic and thermodynamic properties associated with the ultrasound-assisted extraction of anthraquinones were investigated. The results showed that the standard curves for anthraquinones showed excellent linearity from 0.018 to 150 µg mL⁻¹ (<i>r</i> &gt; 0.9998). The detection and quantification limits varied from 5.04 to 5.60 ng mL⁻¹ and 16.81 to 18.67 ng mL⁻¹, respectively. The recoveries were measured as 84.1%–95.1%, and the relative standard deviations were not exceeded 4.6%.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a Simplex Search Framework for the Development of a New Specific and Robust Stability-Indicating Reversed-Phase High-Performance Liquid Chromatography Assay: Application for the Stability of a Peptide-Based Telomerase Vaccine","authors":"Claire Andre,&nbsp;Lydie Lethier,&nbsp;Yves Claude Guillaume","doi":"10.1002/jssc.70111","DOIUrl":"https://doi.org/10.1002/jssc.70111","url":null,"abstract":"<div>\u0000 \u0000 <p>In this work, a novel mathematical algorithm was described for the development of a high-performance liquid chromatography (HPLC) stability-indicating assay. This approach was applied for the stability study of a universal cancer peptide-based telomerase vaccine constituted of a mixture of two peptides. With only 18 experiments, the optimum HPLC gradient conditions were determined using a computer-assisted design of experiments—simplex algorithm software. In addition, the separation on the chromatogram of the isoaspartate peptide degradation product with the active pharmaceutical ingredients was achieved. This HPLC method was validated as per the International Committee on Harmonization guidelines. It was shown that this vaccine was physicochemical stable for 2 months when stored in borosilicate glass vials at −20°C and no formation of isoaspartate peptide was detected. In addition, this vaccine was stable for up to 10 h at 4°C or room temperature after thawing the samples. No impact of potential temperature excursion on the vaccine concentration in the borosilicate glass vial was observed.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Determination of Hormones in Botanical Dietary Supplements With Supramolecular Solvent-Based Extraction by HPLC-MS/MS","authors":"Yasen Qiao,&nbsp;Shuyan Sun,&nbsp;Chuanfeng Huang,&nbsp;Huijun Liu,&nbsp;Yalei Dong,&nbsp;Haiyan Wang,&nbsp;Lei Sun","doi":"10.1002/jssc.70110","DOIUrl":"https://doi.org/10.1002/jssc.70110","url":null,"abstract":"<div>\u0000 \u0000 <p>Dietary supplements typically contain either natural products or synthetic chemicals. While botanical dietary supplements are often thought to be more natural and safer, they could still contain potentially harmful ingredients. Despite the detection of sex hormones in plants and soil, it is still worth examining if products made from plants contain them. In this study, simultaneous determination of 14 kinds of sex hormones in botanical dietary supplements was conducted by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). A suitable sample pretreatment method is expected to extract and purify the sample in a single step when detected by HPLC-MS/MS, enabling it to be directly injected into the instrument. Herein, supramolecular solvent (SUPRAS), composed of <i>n</i>-hexanol, tetrahydrofuran (THF), and water, was employed to extract hormones from botanical dietary supplements. The effect of various parameters, including the composition of the supramolecular solvents, their dosage, and vortex time, on extraction efficiency was investigated. The results demonstrated that the 14 compounds exhibited excellent linearity over the concentration range of 0.5–100 µg·L⁻¹, with determination coefficients exceeding 0.99. Recoveries were assessed at three levels using spiked samples, ranging from 75.6% to 115.8%, with relative standard deviations between 0.8% and 9.7%. This method was successfully applied to determine 14 sex hormones in botanical dietary supplements. Progesterone was detected in one sample at a concentration of 64 µg·kg⁻¹. The SUPRAS method coupled with HPLC-MS/MS, was proved to be efficient, easy-to-operate, and sensitive for determining sex hormones in botanical dietary supplements.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}