Journal of separation science最新文献_第7页

Prediction model for retention volumes of the three-phase solvent system in high-speed countercurrent chromatography 高速逆流色谱中三相溶剂系统保留体积的预测模型

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-31 DOI: 10.1002/jssc.202400385

Jules Muhire, Fu-Xin Zhang, Xiao Sun, Xing-Cui Wang, Dong Pei, Xin-Yi Huang, Duo-Long Di

{"title":"Prediction model for retention volumes of the three-phase solvent system in high-speed countercurrent chromatography","authors":"Jules Muhire, Fu-Xin Zhang, Xiao Sun, Xing-Cui Wang, Dong Pei, Xin-Yi Huang, Duo-Long Di","doi":"10.1002/jssc.202400385","DOIUrl":"https://doi.org/10.1002/jssc.202400385","url":null,"abstract":"Owing to its ability to separate substances with a broad scope of polarities, exploring the three-phase solvent systems (TPSSs) with high-speed countercurrent chromatography is a topic of interest in separation science, and their retention volumes should be more concerned. This study primarily investigates the behavior of retention volumes while examining the isolation abilities of the TPSS in the technique above. We took standard compounds, including sophoricoside, Sudan red 7B, and rotenone, which have a broad range of polarity, for investigation in this study and separated them using different four-liquid TPSSs made up of water, acetonitrile, methyl acetate, and n-hexane (WAMH). Our findings show that the retention volumes gradually alter in response to changes in phase polarity within the proposed solvent systems. With TPSSs, we preliminarily studied compound isolation and the promising formula of their retention volumes. The proposed solvent systems WAMH in different ratios showed high correlations and adjusted correlation coefficients above 0.9978 and 0.9913 for the actual and calculated retention volumes. This study will be particularly beneficial for researchers focusing on countercurrent chromatography with TPSSs, as it offers valuable time-saving insights.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 17","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomorphic synthesis of pore size-tunable mesoporous silica spherical particles and their application for the fraction of low-molecular-weight heparin 孔径可调介孔二氧化硅球形颗粒的拟态合成及其在低分子量肝素分馏中的应用

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-29 DOI: 10.1002/jssc.202400367

Yang Zhao, Jie Li, Yang Yang, Yujie Bi, Changyu Cai, Yanxiong Ke

{"title":"Pseudomorphic synthesis of pore size-tunable mesoporous silica spherical particles and their application for the fraction of low-molecular-weight heparin","authors":"Yang Zhao, Jie Li, Yang Yang, Yujie Bi, Changyu Cai, Yanxiong Ke","doi":"10.1002/jssc.202400367","DOIUrl":"https://doi.org/10.1002/jssc.202400367","url":null,"abstract":"In this study, spherical silica with pore size varied from 30 to 200 Å was synthesized by pseudomorphic transformation at atmospheric pressure. 40–80 Å silica particles with a narrow pore distribution were obtained by using quaternary amine cationic surfactants and different kinds of swelling agents, including polypropylene glycol, 1,3,5-trimethylbenzene, alkanes, and alkanols. Alkyl imidazolium ionic liquid surfactants were used to synthesize large pore size distribution silica spheres with pore sizes in the range of 110–200 Å. All these silica particles can be synthesized under mild conditions within 12 h, which provides a facile synthesis method for the preparation of a chromatographic matrix with tunable pore size. The method is reproducible and the relative standard deviation of silica sphere pore structure parameters in scaled-up preparations is less than 6%. The pore size on the fraction of low-molecular-weight heparin (LMWH) was investigated in size exclusion chromatography. Matrixes with different pore size distributions have various size exclusion regions. By using UPS-60-Diol columns in a twin-column recirculation separation process, LMWH with >85% heparin with molecular weight within the range of 3000–8000 Da were separated in five-column volumes.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 17","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomorphic synthesis of pore size-tunable mesoporous silica spherical particles and their application for the fraction of low-molecular-weight heparin 孔径可调介孔二氧化硅球形颗粒的拟态合成及其在低分子量肝素分馏中的应用

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-29 DOI: 10.1002/jssc.202400367

Yang Zhao, Jie Li, Yang Yang, Yujie Bi, Changyu Cai, Yanxiong Ke

{"title":"Pseudomorphic synthesis of pore size-tunable mesoporous silica spherical particles and their application for the fraction of low-molecular-weight heparin","authors":"Yang Zhao, Jie Li, Yang Yang, Yujie Bi, Changyu Cai, Yanxiong Ke","doi":"10.1002/jssc.202400367","DOIUrl":"https://doi.org/10.1002/jssc.202400367","url":null,"abstract":"In this study, spherical silica with pore size varied from 30 to 200 Å was synthesized by pseudomorphic transformation at atmospheric pressure. 40–80 Å silica particles with a narrow pore distribution were obtained by using quaternary amine cationic surfactants and different kinds of swelling agents, including polypropylene glycol, 1,3,5-trimethylbenzene, alkanes, and alkanols. Alkyl imidazolium ionic liquid surfactants were used to synthesize large pore size distribution silica spheres with pore sizes in the range of 110–200 Å. All these silica particles can be synthesized under mild conditions within 12 h, which provides a facile synthesis method for the preparation of a chromatographic matrix with tunable pore size. The method is reproducible and the relative standard deviation of silica sphere pore structure parameters in scaled-up preparations is less than 6%. The pore size on the fraction of low-molecular-weight heparin (LMWH) was investigated in size exclusion chromatography. Matrixes with different pore size distributions have various size exclusion regions. By using UPS-60-Diol columns in a twin-column recirculation separation process, LMWH with >85% heparin with molecular weight within the range of 3000–8000 Da were separated in five-column volumes.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 17","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of amino acids and peptides without their pre-column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview 采用毛细管电泳法测定氨基酸和肽，无需柱前衍生，紫外检测和非接触电导检测。概述。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-27 DOI: 10.1002/jssc.202400352

Liudmila Kartsova, Anastasiia Maliushevska

引用次数: 0

Characterization of a mixed mode fluorocarbon/weak anion exchange sorbent for the separation of perfluoroalkyl substances 用于分离全氟烷基物质的碳氟化合物/弱阴离子交换混合模式吸附剂的特性。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-27 DOI: 10.1002/jssc.202400413

Aghogho A. Olomukoro, Ruichao Xie, Fabiola X. Fernandez Paucar, Charlotte DeRosa, Neil D. Danielson, Emanuela Gionfriddo

{"title":"Characterization of a mixed mode fluorocarbon/weak anion exchange sorbent for the separation of perfluoroalkyl substances","authors":"Aghogho A. Olomukoro, Ruichao Xie, Fabiola X. Fernandez Paucar, Charlotte DeRosa, Neil D. Danielson, Emanuela Gionfriddo","doi":"10.1002/jssc.202400413","DOIUrl":"10.1002/jssc.202400413","url":null,"abstract":"The ubiquitous presence and persistence of per- and polyfluoroalkyl substances (PFAS) in the environment have raised concerns in the scientific community. Current research efforts are prioritizing effective PFAS remediation through novel sorbents with orthogonal interaction mechanisms. Recognized sorption mechanisms between PFAS and sorbents include hydrophobic, electrostatic, and fluorine-fluorine interaction. The interplay of these mechanisms contributes significantly to improved sorption capacity and selectivity in PFAS separations. In this study, a primary/secondary amine-functionalized polystyrene-divinylbenzene (Sepra-WAX) polymer was modified to create a fluorinated WAX resin (Sepra-WAX-KelF-PEI). The synthesis intermediate (Sepra-WAX-KelF) was also tested to assess the improvement of the final product (Sepra-WAX-KelF-PEI). The adsorption capacity of Sepra-WAX, Sepra-WAX-KelF, and Sepra-WAX-KelF-PEI, and their interactions with PFAS were evaluated. The effect of pH, ionic strength, and organic solvents on PFAS sorption in aqueous solution was also investigated. The sorbents showed varied adsorption capacities for perfluorooctanoic acid, perfluoropentanoic acid, perfluoro-n-decanoic acid, and hexafluoropropylene oxide dimer acid, with the average extraction capacity of the four analytes being Sepra-WAX-KelF-PEI (523 mg/g) > Sepra-WAX (353 mg/g) > Sepra-WAX-KelF (220 mg/g). Sepra-WAX-KelF-PEI provided the highest adsorption capacity for all analytes tested, proving that the combination of electrostatic and hydrophobic/fluorophilic interactions is crucial for the effective preconcentration of PFAS and its future applications for PFAS remediation from aqueous solutions.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 16","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of fluorous affinity using fluoroalkyl-modified silica gel and selective separation of poly-fluoroalkyl substances in organic solvents 评估使用氟烷基改性硅胶的氟亲和性以及有机溶剂中多氟烷基物质的选择性分离。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-27 DOI: 10.1002/jssc.202400121

Atsuya Tadano, Yoshiyuki Watabe, Tetsuya Tanigawa, Sayaka Konishi-Yamada, Takuya Kubo

{"title":"Evaluation of fluorous affinity using fluoroalkyl-modified silica gel and selective separation of poly-fluoroalkyl substances in organic solvents","authors":"Atsuya Tadano, Yoshiyuki Watabe, Tetsuya Tanigawa, Sayaka Konishi-Yamada, Takuya Kubo","doi":"10.1002/jssc.202400121","DOIUrl":"10.1002/jssc.202400121","url":null,"abstract":"In this study, we focused on the fluorous affinity acting among fluorine compounds, and then developed a new separation medium and evaluated their performance. We prepared the stationary phases for a column using silica gel-modified alkyl fluoride and investigated the characteristics of fluorous affinity by comparing them with a typical stationary phase, which does not contain fluorine, using high-performance liquid chromatography (HPLC). In HPLC measurements, we confirmed that while all non-fluorine compounds were not retained, retention of fluorine compounds increased as the number of fluorine increased with the stationary phase. It also revealed that the strength of fluorous affinity changes depending on the types of the organic solvent; the more polar the solvent, the stronger the effect. Additionally, the stationary phase was employed to compare the efficiency of our column with that of a commercially available column, Fluofix-II. The retention selectivity was almost the same, but the absolute retention strength was slightly higher on our column, indicating that the column is available for practical use.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 16","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on pretreatment technology for the analysis of amphetamine biological samples 苯丙胺生物样本分析预处理技术的研究进展。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-27 DOI: 10.1002/jssc.202400337

Zebin Cao, Jianping Zhou

{"title":"Research progress on pretreatment technology for the analysis of amphetamine biological samples","authors":"Zebin Cao, Jianping Zhou","doi":"10.1002/jssc.202400337","DOIUrl":"10.1002/jssc.202400337","url":null,"abstract":"Sample pretreatment technology is crucial for drug analysis and detection, because the effect of sample pretreatment directly determinates the final analysis results. In recent years, with the continuous innovation of microextraction and other technologies like material preparation technologies and assistant technologies for extraction, the sample pretreatment techniques in the process of drug analysis have become more and more mature and diverse. This article takes amphetamine (AM) or methamphetamine as an example to review the recent development of pretreatment methods for AM-containing biological samples from the perspectives of extraction techniques, extraction media and auxiliary technologies. Extraction techniques are summarized with the categories of contact microextraction, separate microextraction and membrane-based microextraction for better guidance of application according to their features. Prevailing and innovative extraction media including carbon-based material, silicon-based material, metal organic framework, molecularly selective materials, supramolecular solvents and ionic liquids are reviewed. Auxiliary technologies like magnetic field, electric field, microwave, ultrasound and so on which can enhance extraction efficiency and accuracy are also reviewed. In the last, prospects of the future development of pretreatment technology for the analysis of AM biological samples are provided.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 16","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generalized flow calculation of the gas flow in a network of capillaries used in gas chromatography 气相色谱法所用毛细管网络中气体流量的通用流量计算。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-23 DOI: 10.1002/jssc.202400419

Jan Leppert, Tillman Brehmer, Peter Boeker, Matthias Wüst

{"title":"Generalized flow calculation of the gas flow in a network of capillaries used in gas chromatography","authors":"Jan Leppert, Tillman Brehmer, Peter Boeker, Matthias Wüst","doi":"10.1002/jssc.202400419","DOIUrl":"10.1002/jssc.202400419","url":null,"abstract":"A general method for the calculation of the flow and pressure of a gas in a network of cylindrical capillaries is presented. This method is used specifically for gas chromatographic systems in this work. With this approach, it is possible to easily calculate flow and pressures in complex gas chromatographic systems, like flow-modulated or thermal-modulated multidimensional gas chromatographic systems, or systems with multiple outlets at different pressures. A mathematic abstraction using graph theory is used to represent the system of capillaries. With this graph, the flow balance equations at the connections of the capillaries can easily be set up. Using a computer algebra system, the system of flow balance equations can be solved for the pressures at the connection points. For simple systems, this approach is presented, and calculated flows, pressures, and hold-up times are compared with measured values. In addition, two complex systems (4-Way-Splitter, Deans Switch system) of capillaries are presented with calculations only. For these systems, certain conditions were formulated, that is, a certain difference in hold-up times and a defined split ratio between different paths of these systems. Using a numeric non-linear solver, configurations of these systems were found, that fulfill these conditions.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 16","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substantial benefits of an inert biphenyl column for the analysis of steroids and their phase II metabolites in biological samples 惰性联苯色谱柱在分析生物样品中类固醇及其第二阶段代谢物方面的巨大优势。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-21 DOI: 10.1002/jssc.202400436

Mathieu Galmiche, Marie-Anaïs Monat, Diego A. Lopez, Cyrille Lamboley, Paul Connolly, Sergey Girel, Davy Guillarme, Isabel Meister, Serge Rudaz

{"title":"Substantial benefits of an inert biphenyl column for the analysis of steroids and their phase II metabolites in biological samples","authors":"Mathieu Galmiche, Marie-Anaïs Monat, Diego A. Lopez, Cyrille Lamboley, Paul Connolly, Sergey Girel, Davy Guillarme, Isabel Meister, Serge Rudaz","doi":"10.1002/jssc.202400436","DOIUrl":"10.1002/jssc.202400436","url":null,"abstract":"Steroids can be used as biomarkers in clinical metabolomics and other fields related to human toxicology. This chemical group is known for its complexity, considering its number of isobaric compounds and the wide variety of phases I and II metabolic pathways that parent compounds can undergo. For a successful analysis of steroids in biological samples, liquid chromatography separation must be finely tuned. It is especially challenging for glucuronidated and sulfated steroids derivatives that bear polar heads and can be affected by non-specific adsorption. The benefits of a biphenyl stationary phase chemistry for the selectivity of the separation of steroids and their phase II metabolites and the extent to which nonspecific adsorption phenomena could degrade chromatographic performance were investigated. Replacing a conventional hardware by a passivated hardware allowed to considerably reduce peaks width and asymmetry of sulfated species. The addition of weak ion pairing agents in the mobile phase could also help to reduce non-specific adsorption but are detrimental to mass spectrometry detection. As confirmed by the successful detection of 52 steroids in plasma, the use of a biphenyl stationary phase complemented by a passivated column hardware is of great help for a successful biomedical analysis of steroids and their phase II metabolites.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 16","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of xanthophylls in egg yolk using a combination of spectrophotometric and diol phase high-performance liquid chromatography methods 采用分光光度法和二元醇相高效液相色谱法测定蛋黄中的黄绿素。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-08-20 DOI: 10.1002/jssc.202400125

Anh Van Nguyen, Victor Ivanovic Deineka, T. G. Burzhinskaya, Irina Petrovna Blinova, Lyudmila Aleksandrovna Deineka, Anh Thi Ngoc Vu

{"title":"Determination of xanthophylls in egg yolk using a combination of spectrophotometric and diol phase high-performance liquid chromatography methods","authors":"Anh Van Nguyen, Victor Ivanovic Deineka, T. G. Burzhinskaya, Irina Petrovna Blinova, Lyudmila Aleksandrovna Deineka, Anh Thi Ngoc Vu","doi":"10.1002/jssc.202400125","DOIUrl":"10.1002/jssc.202400125","url":null,"abstract":"Normal-phase (NP) liquid chromatography is one of the most effective methods for separating isomers with sensitive structural features, including xanthophyll isomers. In this work, reverse-phase (RP) and NP liquid chromatography (LC), with silica gel and diol phase, respectively, were evaluated for the separation of xanthophyll isomers. The results showed that RP LC with monomeric C18 phase not only poorly separate all xanthophyll isomers in egg yolk but also requires additional sample preparation to eliminate triacylglycerols in egg yolk. The diol phase of NP-LC provided the highest efficiency for separating lutein, zeaxanthin, and their cis-isomers with isocratic separation using mobile phases consisting of n-hexane and polar modifiers (such as acetone, methyl tert-butyl ether, or ethyl acetate). To determine the xanthophyll content, peak areas from LC and total absorbance from spectrophotometry measurements were used. The approach was applied to analyze the xanthophylls of nine commercial egg samples. The results revealed that five out of nine analyzed samples contained a high level of canthaxanthin, which contributes to color enhancement but not to prevent age-related macular degeneration. Together, it shows that NP LC with diol phase combined with spectrophotometry is a powerful tool to monitor xanthophylls in eggs.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 16","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0