Journal of separation science最新文献_第7页

Evaporation-free Supercritical Fluid Chromatography—Internal Standard Correction—Solvent Suppression—Quantitative Nuclear Magnetic Resonance for Chiral Separation and Quantification of Quinine 无蒸发超临界液相色谱-内标校正-溶剂抑制-核磁共振手性分离定量研究

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-27 DOI: 10.1002/jssc.70518

Jiaxin Wang, Huan Yao, Ting Huang, Wei Zhang, Zhaoming Ran, Ping Su, Yi Yang

{"title":"Evaporation-free Supercritical Fluid Chromatography—Internal Standard Correction—Solvent Suppression—Quantitative Nuclear Magnetic Resonance for Chiral Separation and Quantification of Quinine","authors":"Jiaxin Wang, Huan Yao, Ting Huang, Wei Zhang, Zhaoming Ran, Ping Su, Yi Yang","doi":"10.1002/jssc.70518","DOIUrl":"https://doi.org/10.1002/jssc.70518","url":null,"abstract":"<div>\u0000 \u0000 <p>Accurate quantification of structurally similar impurities in chiral drugs remains a critical challenge in pharmaceutical quality control. Herein, we established supercritical fluid chromatography—internal standard correction—solvent suppression—quantitative nuclear magnetic resonance (SFC—ISC—SS—qNMR) for the rapid and accurate purity assessment of low-purity quinine (QN). Baseline separation of QN from its chiral isomer quinidine was achieved within 10 min using SFC. The collected eluate, without any nitrogen blowing or freeze-drying, was directly mixed with an IS and a trace amount of deuterium oxide for <sup>1</sup>H qNMR analysis, reducing the total sample preparation time to less than 7 min. An excitation sculpting pulse sequence (zgesgp) was employed to effectively suppress the methanol solvent peak, yielding a flat baseline and an excellent signal-to-noise ratio (S/N > 5000:1). Internal standard correction based on SFC peak area ratios before and after purification eliminated losses and errors during sample preparation. Method validation showed that the determined purity of QN (95.40% ± 0.17%) was in agreement with results obtained by two independent direct qNMR strategies (95.61% ± 0.11% and 95.44% ± 0.23%). Compared with previously reported ISC—high-performance liquid chromatography—qNMR and SFC—ISC—qNMR methods, the proposed approach significantly reduced the sample preparation time, completely avoided the nitrogen blowing or freeze-drying steps, and achieved seamless hyphenation of SFC and qNMR. This strategy provides an efficient, green, and generalizable paradigm for the accurate quantification of low-purity organic compounds in chiral drugs and complex matrices.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaporation-free Supercritical Fluid Chromatography—Internal Standard Correction—Solvent Suppression—Quantitative Nuclear Magnetic Resonance for Chiral Separation and Quantification of Quinine 无蒸发超临界液相色谱-内标校正-溶剂抑制-核磁共振手性分离定量研究

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-27 DOI: 10.1002/jssc.70518

Jiaxin Wang, Huan Yao, Ting Huang, Wei Zhang, Zhaoming Ran, Ping Su, Yi Yang

{"title":"Evaporation-free Supercritical Fluid Chromatography—Internal Standard Correction—Solvent Suppression—Quantitative Nuclear Magnetic Resonance for Chiral Separation and Quantification of Quinine","authors":"Jiaxin Wang, Huan Yao, Ting Huang, Wei Zhang, Zhaoming Ran, Ping Su, Yi Yang","doi":"10.1002/jssc.70518","DOIUrl":"https://doi.org/10.1002/jssc.70518","url":null,"abstract":"<div>\u0000 \u0000 <p>Accurate quantification of structurally similar impurities in chiral drugs remains a critical challenge in pharmaceutical quality control. Herein, we established supercritical fluid chromatography—internal standard correction—solvent suppression—quantitative nuclear magnetic resonance (SFC—ISC—SS—qNMR) for the rapid and accurate purity assessment of low-purity quinine (QN). Baseline separation of QN from its chiral isomer quinidine was achieved within 10 min using SFC. The collected eluate, without any nitrogen blowing or freeze-drying, was directly mixed with an IS and a trace amount of deuterium oxide for <sup>1</sup>H qNMR analysis, reducing the total sample preparation time to less than 7 min. An excitation sculpting pulse sequence (zgesgp) was employed to effectively suppress the methanol solvent peak, yielding a flat baseline and an excellent signal-to-noise ratio (S/N > 5000:1). Internal standard correction based on SFC peak area ratios before and after purification eliminated losses and errors during sample preparation. Method validation showed that the determined purity of QN (95.40% ± 0.17%) was in agreement with results obtained by two independent direct qNMR strategies (95.61% ± 0.11% and 95.44% ± 0.23%). Compared with previously reported ISC—high-performance liquid chromatography—qNMR and SFC—ISC—qNMR methods, the proposed approach significantly reduced the sample preparation time, completely avoided the nitrogen blowing or freeze-drying steps, and achieved seamless hyphenation of SFC and qNMR. This strategy provides an efficient, green, and generalizable paradigm for the accurate quantification of low-purity organic compounds in chiral drugs and complex matrices.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Standards-Aided LC-MS Technologies Uncover 30 “Unreported” Compounds in Natural Bear Bile Powder to Add a New Pharmacopoeia Quality-Marker 两种标准辅助LC-MS技术揭示了天然熊胆粉中30种“未报道”的化合物，增加了一个新的药典质量标记。

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-25 DOI: 10.1002/jssc.70513

Ziqing Li, Haoyu Huang, Heping Zheng, Gongchang Zheng, Xi Zhao, Yongbai Liang, Rongxin Cai, Hanxiao Chai, Hongwei Song, Xican Li

{"title":"Two Standards-Aided LC-MS Technologies Uncover 30 “Unreported” Compounds in Natural Bear Bile Powder to Add a New Pharmacopoeia Quality-Marker","authors":"Ziqing Li, Haoyu Huang, Heping Zheng, Gongchang Zheng, Xi Zhao, Yongbai Liang, Rongxin Cai, Hanxiao Chai, Hongwei Song, Xican Li","doi":"10.1002/jssc.70513","DOIUrl":"10.1002/jssc.70513","url":null,"abstract":"<div>\u0000 \u0000 <p>Natural bear bile powder is a precious traditional Chinese medicine that has a long history of use in clinical practice. The current study analyzed four batches of natural bear bile powder samples (<b>B-1∼B-4</b>) using standards-aided ultra-high-performance liquid chromatography-quadrupole-Orbitrap-mass spectrometry and ultra-performance liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry. A total of 37 compounds (<b>1-37</b>) were accurately identified, while 30 “unreported” compounds were found in natural bear bile powder for the first time. In particular, two “unreported” isomers (2'-hydroxydaidzein and genistein) were strictly distinguished by means of ΔR.T. matching in chromatographic stick-peak and MS/MS peak matching. The quantification work indicated that taurochenodeoxycholic acid (T<span>C</span>DCA) and its epimer tauroursodeoxycholic acid (T<span>U</span>DCA) had the highest contents in natural bear bile powder. However, T<span>C</span>DCA showed different binding energy towards pregnane X receptor from T<span>U</span>DCA in the molecular docking simulation. Based on the analytical chemistry, molecular docking simulation, and documentary evidence, T<span>C</span>DCA was recommended as a potential Q-marker for pharmacopoeia. The new Q-marker was validated to be feasible by analysis of 11 batches of natural bear bile powder samples (<b>B-5∼B-15</b>), and the specific ratio of T<span>C</span>DCA/T<span>U</span>DCA (0.58∼0.98) can distinguish natural BBP from artificial BBP and other animal biles. All these findings not only lay a foundation for manufacturing technique improvement of artificial natural bear bile powder, but also promote pharmacopoeia to update the quality-control method.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 8","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing a Robust High Performance Liquid Chromatography−Ultraviolet Detection Technique for Nitrite Quantification in Pharmaceutical Excipients and Active Pharmaceutical Ingredients 建立高效液相色谱-紫外检测技术定量测定药用辅料和活性药物成分中的亚硝酸盐

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-21 DOI: 10.1002/jssc.70512

Kelly Blundin, Syamantak Roy, Edward Mularz, Junyong Jo

{"title":"Establishing a Robust High Performance Liquid Chromatography−Ultraviolet Detection Technique for Nitrite Quantification in Pharmaceutical Excipients and Active Pharmaceutical Ingredients","authors":"Kelly Blundin, Syamantak Roy, Edward Mularz, Junyong Jo","doi":"10.1002/jssc.70512","DOIUrl":"https://doi.org/10.1002/jssc.70512","url":null,"abstract":"<div>\u0000 \u0000 <p>Nitrite impurities are frequently present in pharmaceutical excipients as a result of raw material sources and manufacturing processes. These impurities are of regulatory concern because nitrite can react with secondary amines to form nitrosamines, a class of potentially mutagenic compounds, making effective control of nitrite critical to ensuring the safety of pharmaceutical products. Current industrial methods for nitrite detection and quantification, such as ion chromatography and related techniques, are often limited by aqueous solvent compatibility, matrix dependence, or restricted instrument availability, which can hinder routine implementation across diverse pharmaceutical materials. In this study, a fast and sensitive analytical approach was developed to address these limitations by enabling nitrite quantification across excipients spanning a broad range of hydrophilicity to hydrophobicity. The method employs a newly developed nitrite isolation strategy from solid pharmaceutical excipient matrices, followed by rapid derivatization to form a UV-active species that can be selectively quantified using high-performance liquid chromatography with ultraviolet detection (HPLC-UV). The resulting workflow demonstrates robust performance for low-level nitrite detection while maintaining compatibility with standard HPLC instrumentation across the diverse ranges of tested pharmaceutical ingredients. The widespread availability of HPLC systems, combined with the method's rapid response, selectivity, and low detection limits for nitrite, supports its adoption for routine analytical control. This approach, therefore, provides a practical tool for nitrite monitoring and mitigation of nitrosamine formation risk in pharmaceutical formulations.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 8","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Characterization of Chemical Constituents in Bamboo Juice Oral Liquid by UHPLC-Q-Exactive Orbitrap MS Combined With Molecular Networking uhplc - q - Orbitrap - MS结合分子网络快速表征竹汁口服液中的化学成分

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-21 DOI: 10.1002/jssc.70511

Yanping Wang, Jieyao Ma, Ci Yang, Yi Zhu, Mingze Xia, Wei Cai

{"title":"Rapid Characterization of Chemical Constituents in Bamboo Juice Oral Liquid by UHPLC-Q-Exactive Orbitrap MS Combined With Molecular Networking","authors":"Yanping Wang, Jieyao Ma, Ci Yang, Yi Zhu, Mingze Xia, Wei Cai","doi":"10.1002/jssc.70511","DOIUrl":"https://doi.org/10.1002/jssc.70511","url":null,"abstract":"<div>\u0000 \u0000 <p>Bamboo juice is a traditional Chinese herbal medicine and drink, and bamboo juice oral liquid (BJOL) is widely marketed in China for clearing away heat, resolving phlegm, and relieving cough. However, the chemical composition has not been fully characterized. This study employed UHPLC-Q-Exactive Orbitrap MS combined with molecular networking to comprehensively characterize the chemical constituents of BJOL. Mass spectrometric data were acquired in both positive and negative ion modes, and the raw data were uploaded to Global Natural Products Social Molecular Networking to construct molecular networking based on the similarity of MS<sup>2</sup> fragmentation patterns. Compound identification was further achieved by comparison with reference standards, database searching, literature verification, and analysis of fragmentation pathways. A total of 121 compounds were identified, including 48 phenylpropanoids, 12 flavonoids, 28 organic acids, 9 amino acids and nucleosides, and 24 other constituents. Among them, 44 compounds were reported in BJOL for the first time. Thus, the UHPLC-Q-Exactive Orbitrap MS strategy established in this study can be applied to the chemical characterization of BJOL, and also provides a reference for investigating its pharmacodynamic components and analyzing constituents of other traditional Chinese medicine preparations.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 8","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Characterization of Chemical Constituents in Bamboo Juice Oral Liquid by UHPLC-Q-Exactive Orbitrap MS Combined With Molecular Networking uhplc - q - Orbitrap - MS结合分子网络快速表征竹汁口服液中的化学成分

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-21 DOI: 10.1002/jssc.70511

Yanping Wang, Jieyao Ma, Ci Yang, Yi Zhu, Mingze Xia, Wei Cai

{"title":"Rapid Characterization of Chemical Constituents in Bamboo Juice Oral Liquid by UHPLC-Q-Exactive Orbitrap MS Combined With Molecular Networking","authors":"Yanping Wang, Jieyao Ma, Ci Yang, Yi Zhu, Mingze Xia, Wei Cai","doi":"10.1002/jssc.70511","DOIUrl":"https://doi.org/10.1002/jssc.70511","url":null,"abstract":"<div>\u0000 \u0000 <p>Bamboo juice is a traditional Chinese herbal medicine and drink, and bamboo juice oral liquid (BJOL) is widely marketed in China for clearing away heat, resolving phlegm, and relieving cough. However, the chemical composition has not been fully characterized. This study employed UHPLC-Q-Exactive Orbitrap MS combined with molecular networking to comprehensively characterize the chemical constituents of BJOL. Mass spectrometric data were acquired in both positive and negative ion modes, and the raw data were uploaded to Global Natural Products Social Molecular Networking to construct molecular networking based on the similarity of MS<sup>2</sup> fragmentation patterns. Compound identification was further achieved by comparison with reference standards, database searching, literature verification, and analysis of fragmentation pathways. A total of 121 compounds were identified, including 48 phenylpropanoids, 12 flavonoids, 28 organic acids, 9 amino acids and nucleosides, and 24 other constituents. Among them, 44 compounds were reported in BJOL for the first time. Thus, the UHPLC-Q-Exactive Orbitrap MS strategy established in this study can be applied to the chemical characterization of BJOL, and also provides a reference for investigating its pharmacodynamic components and analyzing constituents of other traditional Chinese medicine preparations.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 8","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing a Robust High Performance Liquid Chromatography−Ultraviolet Detection Technique for Nitrite Quantification in Pharmaceutical Excipients and Active Pharmaceutical Ingredients 建立高效液相色谱-紫外检测技术定量测定药用辅料和活性药物成分中的亚硝酸盐

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-21 DOI: 10.1002/jssc.70512

Kelly Blundin, Syamantak Roy, Edward Mularz, Junyong Jo

{"title":"Establishing a Robust High Performance Liquid Chromatography−Ultraviolet Detection Technique for Nitrite Quantification in Pharmaceutical Excipients and Active Pharmaceutical Ingredients","authors":"Kelly Blundin, Syamantak Roy, Edward Mularz, Junyong Jo","doi":"10.1002/jssc.70512","DOIUrl":"https://doi.org/10.1002/jssc.70512","url":null,"abstract":"<div>\u0000 \u0000 <p>Nitrite impurities are frequently present in pharmaceutical excipients as a result of raw material sources and manufacturing processes. These impurities are of regulatory concern because nitrite can react with secondary amines to form nitrosamines, a class of potentially mutagenic compounds, making effective control of nitrite critical to ensuring the safety of pharmaceutical products. Current industrial methods for nitrite detection and quantification, such as ion chromatography and related techniques, are often limited by aqueous solvent compatibility, matrix dependence, or restricted instrument availability, which can hinder routine implementation across diverse pharmaceutical materials. In this study, a fast and sensitive analytical approach was developed to address these limitations by enabling nitrite quantification across excipients spanning a broad range of hydrophilicity to hydrophobicity. The method employs a newly developed nitrite isolation strategy from solid pharmaceutical excipient matrices, followed by rapid derivatization to form a UV-active species that can be selectively quantified using high-performance liquid chromatography with ultraviolet detection (HPLC-UV). The resulting workflow demonstrates robust performance for low-level nitrite detection while maintaining compatibility with standard HPLC instrumentation across the diverse ranges of tested pharmaceutical ingredients. The widespread availability of HPLC systems, combined with the method's rapid response, selectivity, and low detection limits for nitrite, supports its adoption for routine analytical control. This approach, therefore, provides a practical tool for nitrite monitoring and mitigation of nitrosamine formation risk in pharmaceutical formulations.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 8","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable Extraction of Mangiferin From Mango Leaves Using Taguchi and Box-Behnken Optimization: Biochemical Characterization and Antioxidant Profiling 田口优化和Box-Behnken优化从芒果叶中可持续提取芒果苷：生化表征和抗氧化分析

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-20 DOI: 10.1002/jssc.70500

Astrid Corrales, Vitor de Pádua Alves, Júlia Naelly Machado Silva, Felipe Rebello Lourenço, Massuo Jorge Kato, André Moreni Lopes

{"title":"Sustainable Extraction of Mangiferin From Mango Leaves Using Taguchi and Box-Behnken Optimization: Biochemical Characterization and Antioxidant Profiling","authors":"Astrid Corrales, Vitor de Pádua Alves, Júlia Naelly Machado Silva, Felipe Rebello Lourenço, Massuo Jorge Kato, André Moreni Lopes","doi":"10.1002/jssc.70500","DOIUrl":"https://doi.org/10.1002/jssc.70500","url":null,"abstract":"<p>Mangiferin (MGF), a bioactive C-glucosylxanthone predominantly found in <i>Mangifera indica</i> L., has attracted increasing interest due to its recognized antioxidant and pharmacological properties. In this study, an aqueous ethanolic maceration process was systematically optimized for MGF recovery from mango plant biomass using a Taguchi experimental design followed by Box-Behnken response surface methodology. The optimized conditions of 39.9°C, 8 h, and 80% v/v ethanol yielded 41.60 mg/g dry weight of MGF, confirming the robustness of the predictive model. Biochemical profiling of the optimized extract revealed 4.36 mg gallic acid equivalents/g dry weight of total phenolics, 73.12 mg/g of reducing sugars, and low protein content, indicating a phytochemical matrix enriched in glycosylated phenolics. Antioxidant evaluation by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay demonstrated 87.74% radical inhibition at 400 µg/mL and a 50% inhibitory concentration of 225 µg/mL, reflecting strong radical scavenging capacity. Greenness assessment using the Analytical GREEnness metric approach software yielded a score of 0.72, validating the environmental compatibility of the proposed method. The results demonstrate that statistically guided aqueous ethanolic maceration represents an efficient, scalable, and sustainable platform for the recovery of MGF-rich extracts with potential pharmaceutical, nutraceutical, and cosmetic applications.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 8","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70500","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable Extraction of Mangiferin From Mango Leaves Using Taguchi and Box-Behnken Optimization: Biochemical Characterization and Antioxidant Profiling 田口优化和Box-Behnken优化从芒果叶中可持续提取芒果苷：生化表征和抗氧化分析

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-20 DOI: 10.1002/jssc.70500

Astrid Corrales, Vitor de Pádua Alves, Júlia Naelly Machado Silva, Felipe Rebello Lourenço, Massuo Jorge Kato, André Moreni Lopes

{"title":"Sustainable Extraction of Mangiferin From Mango Leaves Using Taguchi and Box-Behnken Optimization: Biochemical Characterization and Antioxidant Profiling","authors":"Astrid Corrales, Vitor de Pádua Alves, Júlia Naelly Machado Silva, Felipe Rebello Lourenço, Massuo Jorge Kato, André Moreni Lopes","doi":"10.1002/jssc.70500","DOIUrl":"https://doi.org/10.1002/jssc.70500","url":null,"abstract":"<p>Mangiferin (MGF), a bioactive C-glucosylxanthone predominantly found in <i>Mangifera indica</i> L., has attracted increasing interest due to its recognized antioxidant and pharmacological properties. In this study, an aqueous ethanolic maceration process was systematically optimized for MGF recovery from mango plant biomass using a Taguchi experimental design followed by Box-Behnken response surface methodology. The optimized conditions of 39.9°C, 8 h, and 80% v/v ethanol yielded 41.60 mg/g dry weight of MGF, confirming the robustness of the predictive model. Biochemical profiling of the optimized extract revealed 4.36 mg gallic acid equivalents/g dry weight of total phenolics, 73.12 mg/g of reducing sugars, and low protein content, indicating a phytochemical matrix enriched in glycosylated phenolics. Antioxidant evaluation by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay demonstrated 87.74% radical inhibition at 400 µg/mL and a 50% inhibitory concentration of 225 µg/mL, reflecting strong radical scavenging capacity. Greenness assessment using the Analytical GREEnness metric approach software yielded a score of 0.72, validating the environmental compatibility of the proposed method. The results demonstrate that statistically guided aqueous ethanolic maceration represents an efficient, scalable, and sustainable platform for the recovery of MGF-rich extracts with potential pharmaceutical, nutraceutical, and cosmetic applications.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 8","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70500","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and Selective Extraction and Detection of Baicalin From Scutellariae Radix Using Magnetic Molecularly Imprinted Polymers 磁性分子印迹聚合物快速、选择性提取黄芩中的黄芩苷

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-08-19 DOI: 10.1002/jssc.70507

Jing Li, Yihan Sun, Jialin Guo, Wei Zhao, Xia Liu

{"title":"Rapid and Selective Extraction and Detection of Baicalin From Scutellariae Radix Using Magnetic Molecularly Imprinted Polymers","authors":"Jing Li, Yihan Sun, Jialin Guo, Wei Zhao, Xia Liu","doi":"10.1002/jssc.70507","DOIUrl":"https://doi.org/10.1002/jssc.70507","url":null,"abstract":"<div>\u0000 \u0000 <p>Baicalin (BA), a key flavonoid isolated from <i>Scutellariae Radix</i> (<i>SR</i>), possesses diverse pharmacological activities encompassing anticancer, anti-inflammatory, and hepatoprotective effects. In order to achieve rapid separation and enrichment of BA from complex matrix samples, core–shell magnetic molecularly imprinted polymers (MMIPs) were fabricated using BA as the template molecule and Fe<sub>3</sub>O<sub>4</sub> nanoparticles as the magnetic core. Subsequently, key preparation parameters, including the ratio of functional monomers to cross-linking agents, imprinting time, and the dosage of BA were systematically optimized. Under the optimized conditions, the prepared MMIPs exhibited rapid binding kinetics and a remarkable maximum adsorption capacity of 18.21 mg/g, accompanied by an imprinting factor (IF) of 3.919. Furthermore, the developed MMIPs were successfully employed for the selective extraction and determination of BA from <i>SR</i>. Specifically, the average recoveries of BA at three spiked concentrations (5.00, 11.20, and 20.00 µg/mL) were 107.0%, 92.7%, and 105.2%, respectively, with corresponding relative standard deviations (RSDs) of 5.31%, 1.65%, and 3.28% (<i>n</i> = 3). Overall, this work provides a novel and efficient adsorbent for trace flavonoids enrichment from complex herbal samples, offering a promising pretreatment strategy for active ingredient analysis in traditional Chinese medicine research.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 8","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0