Journal of separation science最新文献_第4页

Preparation of Hybrid Molecularly Imprinted Polymers Based on 3-Triethoxysilylpropyl Methacrylic Amide for Solid-Phase Extraction of Gatifloxacin From Lake Water

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-16 DOI: 10.1002/jssc.70122

Meihong Chai, Chang Liu, Liping Zhang, Yi Wang, Xueyan Zhen, Yi Yang, Yanping Huang, Zhaosheng Liu

{"title":"Preparation of Hybrid Molecularly Imprinted Polymers Based on 3-Triethoxysilylpropyl Methacrylic Amide for Solid-Phase Extraction of Gatifloxacin From Lake Water","authors":"Meihong Chai, Chang Liu, Liping Zhang, Yi Wang, Xueyan Zhen, Yi Yang, Yanping Huang, Zhaosheng Liu","doi":"10.1002/jssc.70122","DOIUrl":"https://doi.org/10.1002/jssc.70122","url":null,"abstract":"<div>\u0000 \u0000 A novel molecularly imprinted polymer (MIP) against gatifloxacin based on a hybrid monomer (3-triethoxysilylpropyl methacrylic amide, APTES-MAA) was reported. In this study, the imprinted monolith was synthesized with gatifloxacin as template, ethylene glycol dimethacrylate (EGDMA) as cross-linker, and APTES-MAA as functional monomer in a mixture of acetonitrile and isooctane as porogen via an optimization based on density functional theory (DFT). The polymers were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and mercury porosimetry. The resulting hybrid MIPs (HMIPs) showed high affinity and selectivity to the template molecule with an imprinted factor of 16.28 ± 0.53, which was 6.1 times higher than that of the traditional methacrylic acid–based MIP. The MIP was used as an adsorbent in solid-phase extraction of lake water with gatifloxacin. The recovery was 96.52 ± 2.69%. The method exhibits the advantageous features of cost-effectiveness and high sensitivity, rendering it a promising approach for analyzing organic pollutants in water.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Chemical Composition of Long-Chain Linear Alkylbenzenes Using High-Performance Liquid Chromatography Coupled with Microwave Plasma Torch Mass Spectrometry

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-16 DOI: 10.1002/jssc.70123

Yuwen He, Jing Luo, Pengcheng Hu, Xingmei Song, Min Zhao, Guozhou Wang, Fengjian Chu, Hongru Feng, Yuanjiang Pan

{"title":"Analysis of Chemical Composition of Long-Chain Linear Alkylbenzenes Using High-Performance Liquid Chromatography Coupled with Microwave Plasma Torch Mass Spectrometry","authors":"Yuwen He, Jing Luo, Pengcheng Hu, Xingmei Song, Min Zhao, Guozhou Wang, Fengjian Chu, Hongru Feng, Yuanjiang Pan","doi":"10.1002/jssc.70123","DOIUrl":"https://doi.org/10.1002/jssc.70123","url":null,"abstract":"<div>\u0000 \u0000 Long-chain linear alkylbenzenes (LABs) are essential precursors to produce alkylbenzene sulfonates, which have high surfactant properties and are readily biodegradable, widely used in various detergents. However, an effective and rapid method for the direct analysis and quality control of LAB chemical composition still needs to be improved. This study uses high-performance liquid chromatography coupled with microwave plasma torch mass spectrometry (HPLC-MPT-MS) to analyze the composition of long-chain LABs and quantify nine of these components. The results show that the main components in long-chain LAB samples are eicosylbenzene, docosylbenzene, and tetracosylbenzene, primarily including four structures, three of which have been structurally identified. This study successfully separates and analyzes LAB directly via HPLC-MPT-MS, providing a valuable and reliable method for the quality evaluation of LAB.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-16 DOI: 10.1002/jssc.70112

Filip Dusa, Marcelina Rusin, Denisa Smolkova, Jozef Sestak, Justyna Dobrowolska-Iwanek, Michał Woźniakiewicz, Jana Lavicka

{"title":"Adapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides","authors":"Filip Dusa, Marcelina Rusin, Denisa Smolkova, Jozef Sestak, Justyna Dobrowolska-Iwanek, Michał Woźniakiewicz, Jana Lavicka","doi":"10.1002/jssc.70112","DOIUrl":"https://doi.org/10.1002/jssc.70112","url":null,"abstract":"The high-sensitivity capabilities of laser-induced fluorescence (LIF) detection continuously promote the development of various labels with different fluorescence properties. However, this strategy also requires the adaptation of existing detection systems to suit the excitation and emission characteristics of novel fluorescent tags. In this study, we adapted the LIF detector of the commercial capillary electrophoresis instrument to the specific fluorescence spectra of 2-aminoacridone labeled human milk oligosaccharides. An external solid-state laser with a wavelength of 405 nm was connected to the commercial capillary electrophoresis instrument via a simple 3D-printed laser-to-light-guide adapter, and different optical filter setups were compared based on the signal-to-noise ratio. The optimized setup provided detection limits as low as 0.27 to 0.34 nM, corresponding to injection of 3.4 to 4.6 attomoles of 2-aminoacridone labeled oligosaccharides. These findings show that the optimized laser and filter configuration can enhance the sensitivity of electrophoretic separation by several orders of magnitude. In addition, the presented setup can be utilized as a guide for coupling different lasers to the commercial instrument.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic Solid-Phase Extraction of Benzoic Acids in Fruit Juices Using Hydrophilic Metal-Organic Framework MIL-101(Cr) Coated with Magnetic Nanoparticles

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-11 DOI: 10.1002/jssc.70118

Yu Jiang, Hong Pan, Menghong Xie, Huifeng Deng, Guihua Ruan, Yipeng Huang

{"title":"Magnetic Solid-Phase Extraction of Benzoic Acids in Fruit Juices Using Hydrophilic Metal-Organic Framework MIL-101(Cr) Coated with Magnetic Nanoparticles","authors":"Yu Jiang, Hong Pan, Menghong Xie, Huifeng Deng, Guihua Ruan, Yipeng Huang","doi":"10.1002/jssc.70118","DOIUrl":"https://doi.org/10.1002/jssc.70118","url":null,"abstract":"<div>\u0000 \u0000 In this study, magnetic Fe3O4-coated metal-organic framework composites, CONH2-MIL-101(Cr)/Fe3O4, were synthesized by depositing Fe3O4 particles on the surface of amidated MIL-101(Cr). The scanning electron microscope images show that the MIL-101(Cr) crystals were uniformly coated by Fe3O4 particles. The magnetic hysteresis curve of CONH2-MIL-101(Cr)/Fe3O4 reveals a saturated magnetization value of 23.47 emu/g. The water contact angle decreases from 61.00° to 12.88° after amidation and Fe3O4 coating. The magnetic solid-phase extraction (MSPE) efficiency of CONH2-MIL-101(Cr)/Fe3O4 for benzoic acid derivatives was studied. Under optimal conditions, the adsorption and desorption processes can be completed within 5 min, and the maximum adsorption capacity to protocatechuic acid (PCA) and vanillic acid (VA) is 10.31 and 17.30 mg/g. Coupling the MSPE with high-performance liquid chromatography-ultraviolet detection, the detection limit for PCA and VA was 0.29 and 0.58 ng/mL, respectively. The mean recoveries of PCA and VA in spiked samples ranged from 82.85%–91.27% with relative standard deviations lower than 0.33%–5.33%. This study provides inspiration for the fabrication of novel magnetic sorbents, and a reliable method for the adsorption and analysis of benzoic acid derivatives.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On-Site Quantification of Transformation Products of Rocket Fuel Unsymmetrical Dimethylhydrazine in Air Using Solid-Phase Microextraction

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-08 DOI: 10.1002/jssc.70113

Bauyrzhan Bukenov, Nurbi Karimkyzy, Aruzhan Smail, Tolkyn Kurmanbayeva, Bulat Kenessov

{"title":"On-Site Quantification of Transformation Products of Rocket Fuel Unsymmetrical Dimethylhydrazine in Air Using Solid-Phase Microextraction","authors":"Bauyrzhan Bukenov, Nurbi Karimkyzy, Aruzhan Smail, Tolkyn Kurmanbayeva, Bulat Kenessov","doi":"10.1002/jssc.70113","DOIUrl":"https://doi.org/10.1002/jssc.70113","url":null,"abstract":"<div>\u0000 \u0000 Despite the high importance of the monitoring of air pollution with transformation products (TPs) of rocket fuel unsymmetrical dimethylhydrazine (UDMH) in the regions of UDMH spills, there is a lack of simple, fast, and accurate methods for quantitation of UDMH TPs in air. This study was aimed at the development of the new method for on-site quantitation of UDMH TPs in air using solid-phase microextraction (SPME) and a portable gas chromatograph with nitrogen–phosphorus detector (GC-NPD). When using a known approach based on SPME from 20-mL vials filled with air, limits of detection (LODs) with a portable GC-NPD ranged from 4 to 10 µg/m3 that is about one order of magnitude higher than with a stationary GC–MS and can be insufficient for detecting UDMH TPs in real air samples. For achieving lower LODs, the sample volume has been increased to 250 mL. According to the modeling and experimental results, the optimal extraction time from 250-mL gas sampling bulbs was 15 min. Increase in the sample volume to 250 mL and extraction time to 15 min provided LODs 0.3–2 µg/m3. To enhance on-site measurements, preliminary screening using 24-h SPME from the liner (internal diameter 4.6 mm) exposed to the open air has been implemented. According to the modeling, such extraction parameters should provide 2.0–3.5 times greater amounts of analytes in the fiber coating. After screening, using this approach on the recent fall place of the first stage of the “Proton-M” carrier rocket, no analytes have been detected.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ionic Liquid-Based Polarity-Adjustable Deep Eutectic Solvent Extraction Followed by High-Performance Liquid Chromatography-Diode-Array Detection for the Determination of Liposoluble Anthraquinones in Salvia miltiorrhiza Bge. Root

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-08 DOI: 10.1002/jssc.70116

Jinghui Li, Yan Kang, Ying Wang, Jiaxin Liu, Yingting Wang, Sitong Liu, Yunxi Bu, Xiangqun Li, Jiahan Xie, Zhibing Wang

{"title":"Ionic Liquid-Based Polarity-Adjustable Deep Eutectic Solvent Extraction Followed by High-Performance Liquid Chromatography-Diode-Array Detection for the Determination of Liposoluble Anthraquinones in Salvia miltiorrhiza Bge. Root","authors":"Jinghui Li, Yan Kang, Ying Wang, Jiaxin Liu, Yingting Wang, Sitong Liu, Yunxi Bu, Xiangqun Li, Jiahan Xie, Zhibing Wang","doi":"10.1002/jssc.70116","DOIUrl":"https://doi.org/10.1002/jssc.70116","url":null,"abstract":"<div>\u0000 \u0000 A simple, green, and efficient ultrasound-assisted deep eutectic solvents (DESs) extraction followed by high-performance liquid chromatography-diode array detector was established to detect four liposoluble anthraquinones in Salvia miltiorrhiza Bge. root. In this study, a series of polarity-adjustable DESs were prepared using n-hexanol and tetrabutylammonium chloride, and their structure, density, viscosity, pH, and thermal stability were determined. The conditions for extraction were optimized through single-factor experiments and response surface methodology. The kinetic and thermodynamic properties associated with the ultrasound-assisted extraction of anthraquinones were investigated. The results showed that the standard curves for anthraquinones showed excellent linearity from 0.018 to 150 µg mL−1 (r > 0.9998). The detection and quantification limits varied from 5.04 to 5.60 ng mL−1 and 16.81 to 18.67 ng mL−1, respectively. The recoveries were measured as 84.1%–95.1%, and the relative standard deviations were not exceeded 4.6%.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of a Simplex Search Framework for the Development of a New Specific and Robust Stability-Indicating Reversed-Phase High-Performance Liquid Chromatography Assay: Application for the Stability of a Peptide-Based Telomerase Vaccine

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-08 DOI: 10.1002/jssc.70111

Claire Andre, Lydie Lethier, Yves Claude Guillaume

{"title":"Construction of a Simplex Search Framework for the Development of a New Specific and Robust Stability-Indicating Reversed-Phase High-Performance Liquid Chromatography Assay: Application for the Stability of a Peptide-Based Telomerase Vaccine","authors":"Claire Andre, Lydie Lethier, Yves Claude Guillaume","doi":"10.1002/jssc.70111","DOIUrl":"https://doi.org/10.1002/jssc.70111","url":null,"abstract":"<div>\u0000 \u0000 In this work, a novel mathematical algorithm was described for the development of a high-performance liquid chromatography (HPLC) stability-indicating assay. This approach was applied for the stability study of a universal cancer peptide-based telomerase vaccine constituted of a mixture of two peptides. With only 18 experiments, the optimum HPLC gradient conditions were determined using a computer-assisted design of experiments—simplex algorithm software. In addition, the separation on the chromatogram of the isoaspartate peptide degradation product with the active pharmaceutical ingredients was achieved. This HPLC method was validated as per the International Committee on Harmonization guidelines. It was shown that this vaccine was physicochemical stable for 2 months when stored in borosilicate glass vials at −20°C and no formation of isoaspartate peptide was detected. In addition, this vaccine was stable for up to 10 h at 4°C or room temperature after thawing the samples. No impact of potential temperature excursion on the vaccine concentration in the borosilicate glass vial was observed.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Determination of Hormones in Botanical Dietary Supplements With Supramolecular Solvent-Based Extraction by HPLC-MS/MS

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-06 DOI: 10.1002/jssc.70110

Yasen Qiao, Shuyan Sun, Chuanfeng Huang, Huijun Liu, Yalei Dong, Haiyan Wang, Lei Sun

{"title":"Simultaneous Determination of Hormones in Botanical Dietary Supplements With Supramolecular Solvent-Based Extraction by HPLC-MS/MS","authors":"Yasen Qiao, Shuyan Sun, Chuanfeng Huang, Huijun Liu, Yalei Dong, Haiyan Wang, Lei Sun","doi":"10.1002/jssc.70110","DOIUrl":"https://doi.org/10.1002/jssc.70110","url":null,"abstract":"<div>\u0000 \u0000 Dietary supplements typically contain either natural products or synthetic chemicals. While botanical dietary supplements are often thought to be more natural and safer, they could still contain potentially harmful ingredients. Despite the detection of sex hormones in plants and soil, it is still worth examining if products made from plants contain them. In this study, simultaneous determination of 14 kinds of sex hormones in botanical dietary supplements was conducted by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). A suitable sample pretreatment method is expected to extract and purify the sample in a single step when detected by HPLC-MS/MS, enabling it to be directly injected into the instrument. Herein, supramolecular solvent (SUPRAS), composed of n-hexanol, tetrahydrofuran (THF), and water, was employed to extract hormones from botanical dietary supplements. The effect of various parameters, including the composition of the supramolecular solvents, their dosage, and vortex time, on extraction efficiency was investigated. The results demonstrated that the 14 compounds exhibited excellent linearity over the concentration range of 0.5–100 µg·L−1, with determination coefficients exceeding 0.99. Recoveries were assessed at three levels using spiked samples, ranging from 75.6% to 115.8%, with relative standard deviations between 0.8% and 9.7%. This method was successfully applied to determine 14 sex hormones in botanical dietary supplements. Progesterone was detected in one sample at a concentration of 64 µg·kg−1. The SUPRAS method coupled with HPLC-MS/MS, was proved to be efficient, easy-to-operate, and sensitive for determining sex hormones in botanical dietary supplements.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Rapid, Detection and Separation of Eugenol and Its Isomers Isolated From Polianthes tuberosa (Linn.) Flower by Supercritical Fluid Chromatography - Photodiode Array-Mass Spectrometry - A Method Development, Validation, and Stability Studies

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-05 DOI: 10.1002/jssc.70109

Kaveesha Srinivasa Suryakoppa, Vivek Hamse Kameshwar, Ramesh Appadurai, Moodgere Habeebulla Moinuddin Khan

{"title":"A Rapid, Detection and Separation of Eugenol and Its Isomers Isolated From Polianthes tuberosa (Linn.) Flower by Supercritical Fluid Chromatography - Photodiode Array-Mass Spectrometry - A Method Development, Validation, and Stability Studies","authors":"Kaveesha Srinivasa Suryakoppa, Vivek Hamse Kameshwar, Ramesh Appadurai, Moodgere Habeebulla Moinuddin Khan","doi":"10.1002/jssc.70109","DOIUrl":"https://doi.org/10.1002/jssc.70109","url":null,"abstract":"<div>\u0000 \u0000 Supercritical fluid chromatography (SFC) is widely recommended as an alternative to normal-phase liquid chromatography in the emerging field of green analytical chemistry. SFC separations are quick, efficient, and environmentally friendly separation of chiral and achiral molecules. Due to this, SFC is economically feasible in separation science technology. The identification and separation of chiral compounds is crucial in understanding their pharmacological significance. Polianthes tuberosa (Linn.) is traditionally considered an ornamental and medicinal plant worldwide. For the separation of eugenol and its derivatives from the P. tuberosa flower, a rapid and highly efficient/SFC approach was developed and implemented in this study. However, no research into the SFC, method validation, and absolute measurement of eugenol has been published from the P. tuberosa flower. We used SFC coupled with mass spectrometry (SFC-MS) to develop a quick and effective approach for characterizing and quantifying eugenol in this investigation. Further, molecular docking studies and molecular dynamic simulations (MDSs) have been performed for the potent isomer of eugenol. This research has uncovered important insights of SFC in drug discovery. Additionally, molecular docking and molecular dynamics simulation studies have provided clarity on how the compound interacts with different biological targets.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Chemical Profiling and Quality Control of Gegen–Danshen Herb Pair Using Advanced Online Two-Dimensional Liquid Chromatography Coupled With Hybrid Linear Ion Trap Orbitrap Mass Spectrometry

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-03-03 DOI: 10.1002/jssc.70107

Xiaohui Deng, Yang Lu, Juan Pan, Qiubing Qin, Shuqi Deng, Qiu Li, Li Wan, Jiliang Cao

{"title":"Comprehensive Chemical Profiling and Quality Control of Gegen–Danshen Herb Pair Using Advanced Online Two-Dimensional Liquid Chromatography Coupled With Hybrid Linear Ion Trap Orbitrap Mass Spectrometry","authors":"Xiaohui Deng, Yang Lu, Juan Pan, Qiubing Qin, Shuqi Deng, Qiu Li, Li Wan, Jiliang Cao","doi":"10.1002/jssc.70107","DOIUrl":"https://doi.org/10.1002/jssc.70107","url":null,"abstract":"<div>\u0000 \u0000 The Gegen–Danshen (GD) herb pair is extensively employed in the treatment of cardiovascular diseases, attributed to its wide spectrum of bioactive constituents. This study presented a comprehensive chemical analysis of GD using an advanced analytical platform, which integrated online two-dimensional liquid chromatography (LC × LC) coupled with diode array detector (DAD) and hybrid linear ion trap orbitrap mass spectrometry (LTQ-orbitrap). In this setup, a Hypersil GOLD CN column (150 mm × 2.1 mm, 3 µm) was employed in the first dimension, while an Accucore PFP column (50 mm × 4.6 mm, 2.6 µm) was used in the second dimension. Under optimized conditions, the online LC × LC-DAD-ESI/HRMS/MSn system exhibited high orthogonality (90.20%) and 102 compounds were identified or tentatively characterized based on high-resolution accurate mass and MSn fragmentation data, acquired in both positive and negative ion modes. Furthermore, a quantitative analysis method was developed and validated using LC × LC-DAD contour plots, targeting 26 bioactive components in GD herb pair. This method demonstrated excellent linearity (R2 ≥ 0.9970) and precision with relative standard deviation (RSD) values below 4.95%. The established analytical approach offers robust separation, reliable identification, and precise quantification of chemical constituents in GD herb pair, providing a valuable tool for the study of bioactive compounds in complex herbal matrices.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0