Journal of separation science最新文献_第4页

Rapid Screening of 25 Environmental Hormones in Aquatic Products Using QuEChERS-Combined With Dispersive Liquid–Liquid Microextraction Based on Solidification of Floating Organic Droplet Coupled With UHPLC-Q/Orbitrap HRMS 基于悬浮有机液滴固化- UHPLC-Q/Orbitrap HRMS联合quechers -分散液-液微萃取快速筛选水产品中25种环境激素

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70206

Jiwang Tang, Yong Xiao, Hongqing Zhao, Can Li, Xiong Hu, Wenbin He

{"title":"Rapid Screening of 25 Environmental Hormones in Aquatic Products Using QuEChERS-Combined With Dispersive Liquid–Liquid Microextraction Based on Solidification of Floating Organic Droplet Coupled With UHPLC-Q/Orbitrap HRMS","authors":"Jiwang Tang, Yong Xiao, Hongqing Zhao, Can Li, Xiong Hu, Wenbin He","doi":"10.1002/jssc.70206","DOIUrl":"https://doi.org/10.1002/jssc.70206","url":null,"abstract":"<div>\u0000 \u0000 An innovative analytical method integrating QuEChERS with dispersive liquid–liquid microextraction using solidified floating organic droplets (DLLME-SFO) and ultra-high-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry (UHPLC-Q/Orbitrap HRMS) was developed for the rapid screening of 25 environmental hormones (EHs) in aquatic products. The method systematically optimized key parameters, including extraction solvent selection (1-undecanol), water volume (5 mL), pH (native pH 6.5), adsorbent combination (C18/PSA, 20 mg each), and salt addition (0% NaCl), achieving high sensitivity with detection limits of 0.1–0.4 µg/kg and recoveries of 68.2%–112.8%. The method demonstrated excellent linearity (R2 > 0.995) across a concentration range of 0.4–40 µg/kg, with precision (intra- and inter-day RSDs < 15%) and manageable matrix effects (−19.5% to 10.9%). The environmental impact assessment, conducted using the Analytical GREEnness Metric for Sample Preparation (AGREEprep score: 0.59) and Blue Applicability Grade Index (BAGI score: 80), confirmed that the proposed method satisfies both sustainability and practicality criteria for EHs analysis. Enhanced matrix purification and dual-polarity HRMS detection enabled accurate identification and quantification of structurally diverse EHs, including pesticides and pharmaceuticals. Validated in 60 commercial samples, the method detected prometryn residues in 11.7% of cases, demonstrating its practicality for monitoring EH contamination in complex aquatic matrices. This approach represents a significant advancement in multi-residue analysis, combining efficiency, sensitivity, and environmental compatibility.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Proteomic and Peptidomic Analysis of Venom From Three Viper Taxa: Evaluating Software-Specific Protein and Peptide Profiles 三种毒蛇类群毒液的比较蛋白质组学和肽组学分析：评估软件特异性蛋白质和肽谱

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70213

Bruno Malheiro, Mert Karış, Bayram Göçmen, Ayse Nalbantsoy, Rui Vitorino

{"title":"Comparative Proteomic and Peptidomic Analysis of Venom From Three Viper Taxa: Evaluating Software-Specific Protein and Peptide Profiles","authors":"Bruno Malheiro, Mert Karış, Bayram Göçmen, Ayse Nalbantsoy, Rui Vitorino","doi":"10.1002/jssc.70213","DOIUrl":"https://doi.org/10.1002/jssc.70213","url":null,"abstract":"<div>\u0000 \u0000 Snake venom is increasingly recognized in biomedical research as a potential source of relevant proteins that are still relatively unknown in various species. In this experiment, we performed proteomic and peptidomic quantification and identification of the venomic profile of three viper taxa: Montivipera blugardaghica subsp. bulgardaghica (MB), Vipera ammodytes subsp. montandoni (VA), and Vipera kaznakovi (VK); and compared the performance of three peptide identification software: PEAKS, MaxQuant, and Proteome Discoverer. Overall, PEAKS identified 19 unique proteins (19 in MB, 11 in VA, and 19 for KV) and 125 unique peptides (55 in MB, 35 in VA, and 63 for VK); MaxQuant identified 577 unique proteins (234 in MB, 275 in VA, and 297 for VK) and 1233 unique peptides (518 in MB, 647 in VA, and 642 for KV); Proteome Discoverer identified 621 unique proteins (310 in MB, 248 for VA, and 346 for VK) and 1657 unique peptides (894 in MB, 830 in VA, and 1041 for VK). The three software shared five identified proteins and 67 peptides; PEAKS shared six proteins and 69 peptides with MaxQuant and six proteins and 79 peptides with Proteome Discoverer; MaxQuant shared 139 proteins and 781 peptides with Proteome Discoverer. All identified proteins were categorized into families for each taxon and then compared with the existing literature. This revealed significant discrepancies in the results between the software and the reviewed literature. Overall, PEAKS performed very poorly, while MaxQuant and Proteome Discoverer performed best for both protein and peptide identification, with the latter software being particularly noteworthy.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graphene Oxide/MXene Nanocomposite Cross-Linked by Urea as a Sorbent for Thin-Film Microextraction of Fipronil and Permethrin and Their Quantification via High-Performance Liquid Chromatography-Ultraviolet Detection 尿素交联氧化石墨烯/MXene纳米复合材料薄膜微萃取氟虫腈和氯菊酯及其高效液相色谱-紫外检测定量研究

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70202

Seyedeh Bentolhoda Hosseinian, Milad Ghani, Jahan Bakhsh Raoof

{"title":"Graphene Oxide/MXene Nanocomposite Cross-Linked by Urea as a Sorbent for Thin-Film Microextraction of Fipronil and Permethrin and Their Quantification via High-Performance Liquid Chromatography-Ultraviolet Detection","authors":"Seyedeh Bentolhoda Hosseinian, Milad Ghani, Jahan Bakhsh Raoof","doi":"10.1002/jssc.70202","DOIUrl":"https://doi.org/10.1002/jssc.70202","url":null,"abstract":"<div>\u0000 \u0000 Herein, a nanocomposite containing graphene oxide/MXene was prepared and cross-linked with urea as a novel sorbent. The sorbent was coated on the surface of a cellulosic paper to prepare a thin film for the thin-film microextraction process. The developed method was applied for the extraction of fipronil and permethrin (as model analytes), followed by separation and quantification using high-performance liquid chromatography-ultraviolet detection. Under optimal condition, the linear dynamic range of the method varied from 0.20 to 100.00 µg L−1, with a correlation coefficient (r2) higher than 0.9967. The limits of detection and limits of quantification were determined to be in the ranges of 0.05–0.06 µg L−1 and 0.17–0.19 µg L−1, respectively. The relative standard deviations (%RSDs) ranged from 2.7% to 5.7%. Additionally, the enrichment factors (EFs) of the studied analytes were 72.00 and 83.00. To evaluate the applicability of the method for real sample analysis, cucumber, tomato, watermelon, and pear were selected and analyzed. The obtained recovery results (90%–116%) confirmed that the method is suitable for accurate analysis.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Molecular Dynamic Simulation in the Enantiorecognition Mechanism of the Pharmaceutically Relevant Leu-Phe Dipeptides With Four Zwitterionic Chiral Stationary Phases 分子动力学模拟在具有四个两性离子手性固定相的药物相关亮氨酸二肽对映体识别机制中的应用

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70220

Ina Varfaj, Roccaldo Sardella, Yana A. Klimova, Leonid D. Asnin, Michael Kohout, Andrea Carotti

{"title":"Application of Molecular Dynamic Simulation in the Enantiorecognition Mechanism of the Pharmaceutically Relevant Leu-Phe Dipeptides With Four Zwitterionic Chiral Stationary Phases","authors":"Ina Varfaj, Roccaldo Sardella, Yana A. Klimova, Leonid D. Asnin, Michael Kohout, Andrea Carotti","doi":"10.1002/jssc.70220","DOIUrl":"https://doi.org/10.1002/jssc.70220","url":null,"abstract":"In order to broaden the applicability of the molecular dynamics technique and to further validate the efficacy of a computational protocol recently developed in our laboratory, the present study aims to elucidate the enantiorecognition mechanisms involving four zwitterionic Cinchona alkaloid-based CSPs under reversed-phase (RP) conditions. In this study, we use the enantiomeric dipeptides D-leucine-D-phenylalanine and L-leucine-L-phenylalanine as probes to investigate the properties of CHIRALPAK ZWIX(+) and ZWIX(-), as well as ZWIX(+A) and ZWIX (−A). The Leu-Phe dipeptide has considerable potential in the pharmaceutical field due to its potential applications in drug delivery, therapeutics and as a building block for peptidomimetics. Furthermore, Leu-Phe is one of the few uncapped dipeptides composed of natural amino acids capable of forming stable hydrogels.The in silico protocol was successfully optimized by setting the simulation box size, run time, and number of frames to record to generate molecular dynamics trajectories as informative as possible. Importantly, the analyses were in complete agreement with the experimental EO, providing insights into the driving forces involved in the enantiorecognition mechanism. In particular, salt bridges and hydrogen bonds were confirmed as the primary interactions, while π–π and π–cation interactions were identified as complementary to facilitate the SO–SA association.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70220","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Proteomic and Peptidomic Analysis of Venom From Three Viper Taxa: Evaluating Software-Specific Protein and Peptide Profiles 三种毒蛇类群毒液的比较蛋白质组学和肽组学分析：评估软件特异性蛋白质和肽谱

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70213

Bruno Malheiro, Mert Karış, Bayram Göçmen, Ayse Nalbantsoy, Rui Vitorino

{"title":"Comparative Proteomic and Peptidomic Analysis of Venom From Three Viper Taxa: Evaluating Software-Specific Protein and Peptide Profiles","authors":"Bruno Malheiro, Mert Karış, Bayram Göçmen, Ayse Nalbantsoy, Rui Vitorino","doi":"10.1002/jssc.70213","DOIUrl":"https://doi.org/10.1002/jssc.70213","url":null,"abstract":"<div>\u0000 \u0000 Snake venom is increasingly recognized in biomedical research as a potential source of relevant proteins that are still relatively unknown in various species. In this experiment, we performed proteomic and peptidomic quantification and identification of the venomic profile of three viper taxa: Montivipera blugardaghica subsp. bulgardaghica (MB), Vipera ammodytes subsp. montandoni (VA), and Vipera kaznakovi (VK); and compared the performance of three peptide identification software: PEAKS, MaxQuant, and Proteome Discoverer. Overall, PEAKS identified 19 unique proteins (19 in MB, 11 in VA, and 19 for KV) and 125 unique peptides (55 in MB, 35 in VA, and 63 for VK); MaxQuant identified 577 unique proteins (234 in MB, 275 in VA, and 297 for VK) and 1233 unique peptides (518 in MB, 647 in VA, and 642 for KV); Proteome Discoverer identified 621 unique proteins (310 in MB, 248 for VA, and 346 for VK) and 1657 unique peptides (894 in MB, 830 in VA, and 1041 for VK). The three software shared five identified proteins and 67 peptides; PEAKS shared six proteins and 69 peptides with MaxQuant and six proteins and 79 peptides with Proteome Discoverer; MaxQuant shared 139 proteins and 781 peptides with Proteome Discoverer. All identified proteins were categorized into families for each taxon and then compared with the existing literature. This revealed significant discrepancies in the results between the software and the reviewed literature. Overall, PEAKS performed very poorly, while MaxQuant and Proteome Discoverer performed best for both protein and peptide identification, with the latter software being particularly noteworthy.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in Dried Microsampling for MS-Based Protein Analysis 质谱法蛋白质分析的干燥微采样研究进展

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70222

Trine Grønhaug Halvorsen, Léon Reubsaet

{"title":"Progress in Dried Microsampling for MS-Based Protein Analysis","authors":"Trine Grønhaug Halvorsen, Léon Reubsaet","doi":"10.1002/jssc.70222","DOIUrl":"https://doi.org/10.1002/jssc.70222","url":null,"abstract":"<div>\u0000 \u0000 The current review provides an overview of the recent progress (2020–January 2025) in dried microsampling for mass spectrometry (MS) based protein analysis. A systematic search was performed to find the literature. Most of the applications are clinical, primarily focusing on targeted biomarker determination. This includes several multiplex methods, allowing simultaneous biomarker evaluation, offering significant advantages over traditional assays. Microsampling is also described in non-targeted proteomics screenings, aiming to discover new biomarkers across different application areas including disease diagnostics and lifestyle monitoring. Especially for the latter, the noninvasive nature is convenient as it enables more frequent sampling. The review discusses the use of microsampling in analysis of samples from animals, the quality of dried microsamples, as well as alternative sampling matrices and sampling materials. Although microsampling is more widely used, challenges such as long response time and tedious sample preparation remain, prompting innovations in the field. One example of this is smart sampling, where enzymatic or antibody functionalities are integrated directly on sampling media, simplifying sample preparation and enhancing analytical precision. As technology advances, dried microsampling in MS based protein analysis is expected to become increasingly important in clinical and research settings. Future innovations are anticipated to expand the utility and extend the adoption of dried microsamples in MS-based protein analyses.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Molecular Dynamic Simulation in the Enantiorecognition Mechanism of the Pharmaceutically Relevant Leu-Phe Dipeptides With Four Zwitterionic Chiral Stationary Phases 分子动力学模拟在具有四个两性离子手性固定相的药物相关亮氨酸二肽对映体识别机制中的应用

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70220

Ina Varfaj, Roccaldo Sardella, Yana A. Klimova, Leonid D. Asnin, Michael Kohout, Andrea Carotti

{"title":"Application of Molecular Dynamic Simulation in the Enantiorecognition Mechanism of the Pharmaceutically Relevant Leu-Phe Dipeptides With Four Zwitterionic Chiral Stationary Phases","authors":"Ina Varfaj, Roccaldo Sardella, Yana A. Klimova, Leonid D. Asnin, Michael Kohout, Andrea Carotti","doi":"10.1002/jssc.70220","DOIUrl":"https://doi.org/10.1002/jssc.70220","url":null,"abstract":"In order to broaden the applicability of the molecular dynamics technique and to further validate the efficacy of a computational protocol recently developed in our laboratory, the present study aims to elucidate the enantiorecognition mechanisms involving four zwitterionic Cinchona alkaloid-based CSPs under reversed-phase (RP) conditions. In this study, we use the enantiomeric dipeptides D-leucine-D-phenylalanine and L-leucine-L-phenylalanine as probes to investigate the properties of CHIRALPAK ZWIX(+) and ZWIX(-), as well as ZWIX(+A) and ZWIX (−A). The Leu-Phe dipeptide has considerable potential in the pharmaceutical field due to its potential applications in drug delivery, therapeutics and as a building block for peptidomimetics. Furthermore, Leu-Phe is one of the few uncapped dipeptides composed of natural amino acids capable of forming stable hydrogels.The in silico protocol was successfully optimized by setting the simulation box size, run time, and number of frames to record to generate molecular dynamics trajectories as informative as possible. Importantly, the analyses were in complete agreement with the experimental EO, providing insights into the driving forces involved in the enantiorecognition mechanism. In particular, salt bridges and hydrogen bonds were confirmed as the primary interactions, while π–π and π–cation interactions were identified as complementary to facilitate the SO–SA association.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70220","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144581968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolutionizing Curcumin Extraction: New Insights From Non-Conventional Methods—A Comparative Analysis of the Last Decade 革命性的姜黄素提取：非常规方法的新见解-近十年的比较分析

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70198

Isabelle O. Torquato, Astrid Corrales, Cassamo U. Mussagy, Jorge F. B. Pereira, André M. Lopes

{"title":"Revolutionizing Curcumin Extraction: New Insights From Non-Conventional Methods—A Comparative Analysis of the Last Decade","authors":"Isabelle O. Torquato, Astrid Corrales, Cassamo U. Mussagy, Jorge F. B. Pereira, André M. Lopes","doi":"10.1002/jssc.70198","DOIUrl":"https://doi.org/10.1002/jssc.70198","url":null,"abstract":"Curcumin (CCM), derived from Curcuma longa L. rhizomes, holds significant pharmaceutical and biotechnological potential, driving extensive scientific research. Beyond its potent biological properties, the extraction of CCM has attracted considerable attention. Although numerous review articles have explored CCM extraction, a gap remains in the identification and comparison of methods aimed at improving these processes. This review seeks to address this gap by discussing efficient extraction methods that align with the principles of sustainability. It is essential to recognize that the effectiveness of both conventional and non-conventional extraction methods depends not only on the techniques themselves but also on the optimal combination of these methods with suitable solvents. Conventional solid–liquid extraction methods often rely on volatile organic compounds which, despite their widespread industrial use, present several limitations, including low-to-moderate extraction yields, prolonged extraction times, the risk of thermal degradation or chemical alteration of the target molecule, and significant environmental impact. In contrast, non-conventional, eco-friendly methods, such as ultrasound-, microwave-, and enzyme-assisted extraction, and supercritical fluid extraction, have garnered increasing attention. When combined with sustainable, neoteric solvents, these methods offer enhanced efficiency, selectivity, and environmental advantages. Future research should focus on optimizing and integrating these innovative extraction methods with green solvents to improve CCM yield, reduce environmental impact, and enhance scalability, ensuring alignment with sustainable practices in bioindustries.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70198","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graphene Oxide/MXene Nanocomposite Cross-Linked by Urea as a Sorbent for Thin-Film Microextraction of Fipronil and Permethrin and Their Quantification via High-Performance Liquid Chromatography-Ultraviolet Detection 尿素交联氧化石墨烯/MXene纳米复合材料薄膜微萃取氟虫腈和氯菊酯及其高效液相色谱-紫外检测定量研究

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70202

Seyedeh Bentolhoda Hosseinian, Milad Ghani, Jahan Bakhsh Raoof

{"title":"Graphene Oxide/MXene Nanocomposite Cross-Linked by Urea as a Sorbent for Thin-Film Microextraction of Fipronil and Permethrin and Their Quantification via High-Performance Liquid Chromatography-Ultraviolet Detection","authors":"Seyedeh Bentolhoda Hosseinian, Milad Ghani, Jahan Bakhsh Raoof","doi":"10.1002/jssc.70202","DOIUrl":"https://doi.org/10.1002/jssc.70202","url":null,"abstract":"<div>\u0000 \u0000 Herein, a nanocomposite containing graphene oxide/MXene was prepared and cross-linked with urea as a novel sorbent. The sorbent was coated on the surface of a cellulosic paper to prepare a thin film for the thin-film microextraction process. The developed method was applied for the extraction of fipronil and permethrin (as model analytes), followed by separation and quantification using high-performance liquid chromatography-ultraviolet detection. Under optimal condition, the linear dynamic range of the method varied from 0.20 to 100.00 µg L−1, with a correlation coefficient (r2) higher than 0.9967. The limits of detection and limits of quantification were determined to be in the ranges of 0.05–0.06 µg L−1 and 0.17–0.19 µg L−1, respectively. The relative standard deviations (%RSDs) ranged from 2.7% to 5.7%. Additionally, the enrichment factors (EFs) of the studied analytes were 72.00 and 83.00. To evaluate the applicability of the method for real sample analysis, cucumber, tomato, watermelon, and pear were selected and analyzed. The obtained recovery results (90%–116%) confirmed that the method is suitable for accurate analysis.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolutionizing Curcumin Extraction: New Insights From Non-Conventional Methods—A Comparative Analysis of the Last Decade 革命性的姜黄素提取：非常规方法的新见解-近十年的比较分析

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-07-08 DOI: 10.1002/jssc.70198

Isabelle O. Torquato, Astrid Corrales, Cassamo U. Mussagy, Jorge F. B. Pereira, André M. Lopes

{"title":"Revolutionizing Curcumin Extraction: New Insights From Non-Conventional Methods—A Comparative Analysis of the Last Decade","authors":"Isabelle O. Torquato, Astrid Corrales, Cassamo U. Mussagy, Jorge F. B. Pereira, André M. Lopes","doi":"10.1002/jssc.70198","DOIUrl":"https://doi.org/10.1002/jssc.70198","url":null,"abstract":"Curcumin (CCM), derived from Curcuma longa L. rhizomes, holds significant pharmaceutical and biotechnological potential, driving extensive scientific research. Beyond its potent biological properties, the extraction of CCM has attracted considerable attention. Although numerous review articles have explored CCM extraction, a gap remains in the identification and comparison of methods aimed at improving these processes. This review seeks to address this gap by discussing efficient extraction methods that align with the principles of sustainability. It is essential to recognize that the effectiveness of both conventional and non-conventional extraction methods depends not only on the techniques themselves but also on the optimal combination of these methods with suitable solvents. Conventional solid–liquid extraction methods often rely on volatile organic compounds which, despite their widespread industrial use, present several limitations, including low-to-moderate extraction yields, prolonged extraction times, the risk of thermal degradation or chemical alteration of the target molecule, and significant environmental impact. In contrast, non-conventional, eco-friendly methods, such as ultrasound-, microwave-, and enzyme-assisted extraction, and supercritical fluid extraction, have garnered increasing attention. When combined with sustainable, neoteric solvents, these methods offer enhanced efficiency, selectivity, and environmental advantages. Future research should focus on optimizing and integrating these innovative extraction methods with green solvents to improve CCM yield, reduce environmental impact, and enhance scalability, ensuring alignment with sustainable practices in bioindustries.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70198","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0