Journal of separation science最新文献_第5页

Construction of α1A-Adrenoceptor-Based Stationary Phase Triggered by the Ultrahigh Affinity Protein Pair Colicin E7 Dnase /IM7 for Targeted Bioactive Compound Screening in Herba leonuri 超亲和蛋白对Colicin E7 Dnase /IM7触发α 1a -肾上腺素受体固定相的构建及其在益母草靶向活性化合物筛选中的应用

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-07 DOI: 10.1002/jssc.70524

Xiaofang Qiao, Haosen Zhang, Yue Zhang, Qian Liu, Jia Guo, Jing Wang

{"title":"Construction of α1A-Adrenoceptor-Based Stationary Phase Triggered by the Ultrahigh Affinity Protein Pair Colicin E7 Dnase /IM7 for Targeted Bioactive Compound Screening in Herba leonuri","authors":"Xiaofang Qiao, Haosen Zhang, Yue Zhang, Qian Liu, Jia Guo, Jing Wang","doi":"10.1002/jssc.70524","DOIUrl":"10.1002/jssc.70524","url":null,"abstract":"<div>\u0000 \u0000 Immobilization of functional proteins onto solid surfaces is pivotal to propel the advancement of biomaterials in both biomedical and industrial technologies. However, the process has been hindered when it comes to G protein-coupled receptors due to the homogeneity of the seven transmembrane proteins. Herein, we exploited the efficient ultrahigh affinity of colicin E7 (CL7) and its cognate immunity protein IM7 for conjugation of functional protein onto macro-porous silica gel and established a robust chromatographic system for separation of compounds from complex matrices. Benefiting from the ultrahigh affinity nature of the protein pair, conjugation of α1A-adrenoceptor (α1A-AR) was achieved at ambient conditions in less than 20 min with high specificity and stability. Feasibility of the platform was tested by determining the binding parameters between drugs and receptor under various conditions (different pHs and concentration of methanol). Under the desired condition, carvedilol gave the association constant of (11.56±0.7) × 105 M−1 indicating immobilization of α1A-AR via this method still enhances its binding activity to drugs. Furthermore, stachydrine and leonurine were obtained as the bioactive compounds targeting the immobilized α1A-AR. Our study is expected to offer a versatile platform to achieve effective and specific immobilization of other functional proteins, thus enabling easy separation of the target compound from complex systems.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly Imprinted Polymers for Steroid Hormone Analysis: Design Strategies, Recognition Mechanisms, and Detection Applications 用于类固醇激素分析的分子印迹聚合物：设计策略、识别机制和检测应用。

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-03 DOI: 10.1002/jssc.70522

Yan Wang, Yuxin Guo, Xiaotong Fu, Hui Li, Wei Wu

{"title":"Molecularly Imprinted Polymers for Steroid Hormone Analysis: Design Strategies, Recognition Mechanisms, and Detection Applications","authors":"Yan Wang, Yuxin Guo, Xiaotong Fu, Hui Li, Wei Wu","doi":"10.1002/jssc.70522","DOIUrl":"10.1002/jssc.70522","url":null,"abstract":"<div>\u0000 \u0000 Steroid hormones are important trace bioactive compounds found in food, environmental, biological, and regulatory samples. However, their accurate analysis remains challenging due to their low concentration, strong hydrophobicity, highly similar structures, and susceptibility to interference from complex matrices. Molecularly imprinted polymers contain binding sites designed to complement target molecules in size, shape, and functional-group arrangement and have been applied to the selective enrichment and detection of steroid hormones. This review examines steroid hormone molecularly imprinted polymers with emphasis on hormone structure, template and monomer selection, cross-linkers, porogens, and surface or magnetic imprinting strategies. Furthermore, it summarizes the integrated applications of molecularly imprinted polymers with electrochemistry, optics, quartz crystal microbalances, surface plasmon resonance, photonic crystals, and wearable sensing platforms, and reviews their current applications in environmental monitoring, food safety analysis, clinical testing, and biomonitoring. Key priorities are rational imprint design, matrix-compatible interfaces, and standardized performance evaluation to support the use of steroid hormone molecularly imprinted polymers in complex samples.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Networking-Based Annotation of Tuberostemonine Isomers Using MS/MS Spectral Similarity. 基于分子网络的MS/MS光谱相似度标注薯蓣皂苷异构体

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-01 DOI: 10.1002/jssc.70536

Xue-Ting Peng, Bo-Hua Li, Chang Su, Chen Zhang

引用次数: 0

Online Enrichment and Separation of Phthalides and Phenolic Acids From Chuanxiong Rhizoma by Reversed Migration Cyclodextrin-Modified Micellar Electrokinetic Chromatography Combined With a Three-Step Enrichment Technique. 反迁移环糊精修饰胶束电动色谱-三步富集技术在线富集分离川芎中邻苯酞和酚酸类物质。

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-01 DOI: 10.1002/jssc.70534

Liping Huang, Wenping Liu, Jin Li, Xiaoyan Wang, Yaqi Yao, Yanxu Chang

{"title":"Online Enrichment and Separation of Phthalides and Phenolic Acids From Chuanxiong Rhizoma by Reversed Migration Cyclodextrin-Modified Micellar Electrokinetic Chromatography Combined With a Three-Step Enrichment Technique.","authors":"Liping Huang, Wenping Liu, Jin Li, Xiaoyan Wang, Yaqi Yao, Yanxu Chang","doi":"10.1002/jssc.70534","DOIUrl":"10.1002/jssc.70534","url":null,"abstract":"A sensitive three-step online enrichment strategy including large-volume sample stacking, dynamic pH junction, and sweeping was successfully developed and validated for the simultaneous online enrichment and separation of phthalides and phenolic acids in Chuanxiong Rhizoma (CX) by coupling reversed migration cyclodextrin-modified micellar electrokinetic chromatography (RM-CD-MEKC) with a diode array detector. The samples were prepared in a low-conductivity, high-pH matrix consisting of 3 mM borax buffer at pH 7.5. The background electrolyte was a high-conductivity, low-pH buffer containing 10 mg·mL-1 sulfobutylether-β-cyclodextrin, 80 mM sodium dodecyl sulfate, and 25 mM sodium dihydrogen phosphate in 20% methanol (pH 3.5). Under the optimized conditions, four major active components (senkyunolide A, senkyunolide I, ferulic acid, and chlorogenic acid) of Ligusticum chuanxiong Hort. were efficiently separated within 17 min, achieving good resolution and 10- to 44-fold enrichment. The limits of detection ranged from 0.09 to 0.25 µg·mL-1 while the limits of quantification ranged from 0.31 to 2.24 µg·mL-1. The relative standard deviations for intra-day and inter-day precision were all below 3.45%, demonstrating good precision and method stability. Overall, the proposed RM-CD-MEKC combined with a three-step enrichment technique provides a simple, rapid, and environmentally friendly enrichment approach for the simultaneous determination of phthalides and phenolic acids in CX samples.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":"e70534"},"PeriodicalIF":3.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13584848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "Development and validation of high-performance liquid chromatography method for determination of clarithromycin in pharmaceutical tablets". 修正了“高效液相色谱法测定片剂中克拉霉素含量的建立与验证”。

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-01 DOI: 10.1002/jssc.70519

引用次数: 0

Mechanistic Origins of Pressure Reduction, Apparent Efficiency Gain, and Enhanced Detector Response in Radial Flow Stream Splitting Chromatography. 径向流分裂色谱中压力降低、表观效率增益和检测器响应增强的机理起源。

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-01 DOI: 10.1002/jssc.70532

Jake A Cravino, Ming Zhao, Torgny Fornstedt, Jorgen Samuelsson, Arianne Soliven, Richard Henry, Ross Andrew Shalliker

{"title":"Mechanistic Origins of Pressure Reduction, Apparent Efficiency Gain, and Enhanced Detector Response in Radial Flow Stream Splitting Chromatography.","authors":"Jake A Cravino, Ming Zhao, Torgny Fornstedt, Jorgen Samuelsson, Arianne Soliven, Richard Henry, Ross Andrew Shalliker","doi":"10.1002/jssc.70532","DOIUrl":"10.1002/jssc.70532","url":null,"abstract":"Radial flow stream (RFS) splitting has been reported to reduce operating pressure and apparent theoretical plate height (increasing column efficiency) while increasing detector response relative to conventional high-performance liquid chromatography column operation. However, the mechanisms responsible for these effects have remained unclear. Here, pressure measurements, computational fluid dynamics, and reduced-order outlet models were used to examine how radial outlet partitioning in the new end fitting alters column behavior. Most of the measured pressure reduction arose from post-column effects, although a smaller in-column contribution remained. Simulations showed that the radial outlet disrupted the conventional outlet-flow funnel and preferentially captured wall-adjacent flow. When the outlet models were applied to a published model of radial column heterogeneity, apparent column plate height decreased by approximately 19% and peak height increased by approximately 18%, consistent with typical experimental results. Experimental sensitivity improvements also persisted under detector conditions that were independent of flow rate. These results indicate that the reported benefits under RFS splitting mode arise from a combination of post-column pressure effects and selective sampling of the radial solute distribution.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":"e70532"},"PeriodicalIF":3.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13583410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Dual Approach Combining Metal-Organic Frameworks and Commercial Solid-Phase Microextraction Coatings for Monitoring Volatiles in Passion Fruit. 金属有机框架和商用固相微萃取涂层相结合的双方法监测百香果中挥发物。

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-01 DOI: 10.1002/jssc.70543

Montse Saura-Cayuela, Martina Rosso, Cecilia Cagliero, Jorge Pasán, Juan H Ayala, Verónica Pino, María J Trujillo-Rodríguez

{"title":"A Dual Approach Combining Metal-Organic Frameworks and Commercial Solid-Phase Microextraction Coatings for Monitoring Volatiles in Passion Fruit.","authors":"Montse Saura-Cayuela, Martina Rosso, Cecilia Cagliero, Jorge Pasán, Juan H Ayala, Verónica Pino, María J Trujillo-Rodríguez","doi":"10.1002/jssc.70543","DOIUrl":"10.1002/jssc.70543","url":null,"abstract":"This study presents the applicability of a solid-phase microextraction (SPME) coating based on the CIM-80(Al) metal-organic framework (MOF), prepared by in situ solvothermal growth onto braided silver wires, for the untargeted analysis of passion fruit juices. After the characterization of the MOF-coated fiber by Fourier-transform infrared spectroscopy, scanning electron microscopy, and powder X-ray diffraction, it was applied in both headspace (HS)- and direct immersion (DI)- SPME in combination with gas chromatography-mass spectrometry. The studies were carried out in comparison with three commercial SPME fibers. Considering all the fibers studied, up to 52 and 26 compounds were tentatively identified via HS- and DI-SPME, respectively. Besides, all fibers presented adequate reproducibility and similar lifetime, even in the DI mode and despite the sample complexity. In HS-SPME, a total of 25 compounds were tentatively identified with the MOF-coated fiber. From these analytes, 10 compounds (∼42%) were exclusively detected with the MOF-coated fiber. In DI-SPME, 10 compounds were tentatively identified with the MOF-coated fiber, and four of these compounds (∼50%) were exclusively detected with this fiber. Overall, the results indicate that the MOF-coated fiber offers a superior selectivity for heavier, less volatile, and less polar analytes, therefore acting as a complementary tool for commercial coatings when intending metabolomic studies for food analysis. The dual approach with MOF-coated fiber and commercial SPME coatings provides more comprehensive information for food analysis.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":"e70543"},"PeriodicalIF":3.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic Covalent Organic Framework for the Simultaneous Extraction of Eight Sulfonamide Antibiotics in Fish and Chicken Coupled With Capillary Electrophoresis Analysis. 磁性共价有机框架同时提取鱼和鸡中8种磺胺类抗生素及毛细管电泳分析。

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-01 DOI: 10.1002/jssc.70542

Ailing Zhu, Mingfang Wu, Ruichengwen Tao, Huige Zhang, Hongying Du, Hongli Chen

{"title":"Magnetic Covalent Organic Framework for the Simultaneous Extraction of Eight Sulfonamide Antibiotics in Fish and Chicken Coupled With Capillary Electrophoresis Analysis.","authors":"Ailing Zhu, Mingfang Wu, Ruichengwen Tao, Huige Zhang, Hongying Du, Hongli Chen","doi":"10.1002/jssc.70542","DOIUrl":"https://doi.org/10.1002/jssc.70542","url":null,"abstract":"As a kind of adsorption material with excellent properties, covalent organic frameworks (COFs) have a broad application prospect in the field of sample pretreatment. Herein, a magnetic COF was constructed by combining magnetic nanospheres with a stable azine-bonded COF via the monomer-mediated in-situ growth strategy for the enrichment and determination of sulfonamide antibiotics. The magnetic COF has good magnetic properties, an appropriate specific surface area, and high stability, and is an excellent adsorbent for magnetic solid-phase extraction. The adsorption mechanism study showed that the large conjugated system and the rich N element of the azine-bonded COF provided abundant π-π interactions and hydrogen-bonding sites, which were beneficial to its interaction with sulfonamide antibiotics. An analytical method for the detection of eight sulfonamides in fish and chicken was developed by combining magnetic extraction with capillary electrophoresis analysis. Under the optimal conditions, the proposed method showed good linearity between the peak areas and the concentrations of eight sulfonamides from 0.1 to 5.0 µg/mL, with correlation coefficients greater than 0.98 and limits of detection from 0.008 to 0.057 µg/mL. This work provides an alternative method to capture and detect sulfonamide antibiotics that is highly efficient, rapid, and sensitive.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":"e70542"},"PeriodicalIF":3.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149061884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of Polar-Organic HPLC, Reversed-Phase HPLC, and Supercritical Fluid Chromatography for Enantioseparation and Gram-Scale Purification of Meclizine and Buclizine. 极性有机高效液相色谱法、反相高效液相色谱法和超临界液相色谱法在美甲利嗪和布利嗪对映体分离和克级纯化中的比较评价。

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-01 DOI: 10.1002/jssc.70538

Srikanth Mannem, Srinuvasu Nakka, Rupak Raja, Nagaraju Devunuri

{"title":"Comparative Evaluation of Polar-Organic HPLC, Reversed-Phase HPLC, and Supercritical Fluid Chromatography for Enantioseparation and Gram-Scale Purification of Meclizine and Buclizine.","authors":"Srikanth Mannem, Srinuvasu Nakka, Rupak Raja, Nagaraju Devunuri","doi":"10.1002/jssc.70538","DOIUrl":"https://doi.org/10.1002/jssc.70538","url":null,"abstract":"Rapid enantioseparation and scalable purification processes of chiral drug molecules are crucial to drug development and quality control (QC) but still challenging. Supercritical fluid chromatography is one of the emerging approaches for quicker turnaround times and high throughput to solve these issues. In this study, we report an organized comparison of polar organic HPLC (PO-HPLC), conventional reversed-phase HPLC (RP-HPLC), and supercritical fluid chromatography (SFC) to perform the enantioseparation and gram-scale purification of antihistamine drugs Meclizine (I) and Buclizine (II) with a common polysaccharide-based chiral stationary phase (Chiralpak IJ column). Analytical performance was assessed by examining the retention behavior, resolution, peak symmetry, runtime, and solvent consumption. Effective baseline separations were obtained with polar-organic and reversed-phase HPLC, but longer times for analysis and more organic solvent usage were needed. Chiral SFC, on the other hand, provided quick analyses (5-6 min) with higher resolution (Rs > 2.2), improved peak shapes, and significantly lower organic solvent consumption. Preparative separations of all three techniques were demonstrated at the 1 g scale. Chiral SFC provided the highest overall productivity, with >99% enantiomeric excess and >90% recovery in less than 3 h, while greatly reducing post run work up. The analytical chiral SFC method validation was done according to ICH Q2 (R2) requirements, confirming its specificity, linearity, precision, accuracy, robustness, and solution stability. Taken together, the results illustrate chiral SFC as the most efficient and sustainable platform for analytical and preparative enantioseparation of meclizine and buclizine, with complementary roles for polar organic and reversed phase HPLC.","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":"e70538"},"PeriodicalIF":3.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149061881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Metabolism-Oriented Strategy for Systematic Analysis of the Pharmacological Material Basis of Classic Traditional Chinese Medicine Formulas: Taking Wuao Sanzi Decoction as an Example 以代谢学为导向的中药经典方剂药理物质基础系统分析——以五奥三子汤为例

IF 3.6 3区工程技术

Journal of separation science Pub Date : 2026-09-01 DOI: 10.1002/jssc.70521

Jin-Jin Liao, Yang-Dan Liu, Lan-Ying Gao, Gui-Zhong Xin, Yuan Cao, Zi-Qi Shi

{"title":"A Metabolism-Oriented Strategy for Systematic Analysis of the Pharmacological Material Basis of Classic Traditional Chinese Medicine Formulas: Taking Wuao Sanzi Decoction as an Example","authors":"Jin-Jin Liao, Yang-Dan Liu, Lan-Ying Gao, Gui-Zhong Xin, Yuan Cao, Zi-Qi Shi","doi":"10.1002/jssc.70521","DOIUrl":"10.1002/jssc.70521","url":null,"abstract":"<div>\u0000 \u0000 Deciphering the pharmacodynamic material basis of classical traditional Chinese medicine (TCM) formulas remains a critical challenge, owing to their multicomponent nature, multitarget interactions, and intricate in vivo metabolic fates. To address this issue, a metabolism-oriented strategy was established and applied to Wuao Sanzi Decoction (WASZD), a classic TCM formula for respiratory diseases such as asthma. Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was used for systematic analysis of the chemicalome, in vivo prototypes, metabolites, and tissue distribution of WASZD. A total of 200 chemical components were identified in vitro, including 102 flavonoids, 29 triterpenoids, 10 steroids, 14 organic acids, and 45 other compounds. Further, 91 prototypes and 212 metabolites in vivo were characterized in biological samples of rats. Key metabolic pathways involved phase I reactions (reduction, oxidation, and demethylation), phase II reactions (glucuronidation, methylation, and sulfation), and gut microbiota-mediated ring cleavage of flavonoids, which generated bioactive small-molecule phenolic acids. Tissue distribution analysis revealed that prototypes and metabolites were predominantly enriched in the ileum, colon, liver, kidney, and lung, with flavonoids and their metabolites identified as the most abundant components, suggesting they are the core bioactive candidates of WASZD. This study not only putatively clarifies the pharmacodynamic material basis of WASZD but also establishes a reproducible technical framework for decoding the complex in vivo behavior of multi-component TCM formulas, facilitating their modernization and rational clinical application.\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"49 9","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0