Journal of separation science最新文献_第5页

Purification, Source Exploration, and Bioactivity Evaluation of Oligosaccharides in Sijunzi Decoction 四君子汤中低聚糖的纯化、探源及生物活性评价

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-28 DOI: 10.1002/jssc.202400407

Xiaonan Chen, Zongjin Pu, Yixin Liu, Bangjian Dong, Xiaobo Li

{"title":"Purification, Source Exploration, and Bioactivity Evaluation of Oligosaccharides in Sijunzi Decoction","authors":"Xiaonan Chen, Zongjin Pu, Yixin Liu, Bangjian Dong, Xiaobo Li","doi":"10.1002/jssc.202400407","DOIUrl":"10.1002/jssc.202400407","url":null,"abstract":"<div>\u0000 \u0000 <p>Sijunzi decoction (SJZD) has been widely used to treat splenic deficiency syndrome. Previous studies confirmed that polysaccharides and non-polysaccharides (NPS) are the main active components of SJZD. This study aimed to investigate the composition and activity of oligosaccharides in NPS. In this study, the oligosaccharide component (named SJZD-OGS), consisting of several different oligosaccharides, was separated and purified from non-polysaccharides of SJZD (SJZD_NPS). Ultra-high-performance liquid chromatography–ion mobility spectrometry–quadrupole time-of-flight mass spectrometry (UHPLC–IMS–QTOF/MS), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) were used to analyze SJZD-OGS. SJZD-OGS, with a molecular weight of 2178 Da, was composed of glucose and fructose, and its chemical composition mainly included fructo-oligosaccharides (FOS) with a degree of polymerization of 3–14 (DP 3–14). The results of oligosaccharide source exploration demonstrated that the chemical composition of SJZD-OGS was different from that of the oligosaccharides of each herb in SJZD. In addition, SJZD-OGS showed good anti-inflammatory activity on macrophage cells and intestinal epithelial cell protective activity. The present work provides experimental evidence for elucidating the pharmaceutical components of SJZD and presents an effective strategy for the research of oligosaccharides in traditional Chinese medicine (TCM).</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 19","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A Polymer-Based Polar Stationary Phase Grafted With Modified Lysine for Hydrophilic Interaction Chromatography 接枝了改性赖氨酸的聚合物型极性固定相，用于亲水相互作用色谱法

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-25 DOI: 10.1002/jssc.202400521

Yufeng Shen, Rui Jin, Feifang Zhang, Bingcheng Yang

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Facile Synthesis of Aptamer-Functionalized Polydopamine-Coated Magnetic Graphene Oxide Nanocomposites for Highly Efficient Purification of His-Tagged Proteins 用于高效纯化 His 标记蛋白质的 Aptamer 功能化聚多巴胺包覆磁性石墨烯氧化物纳米复合材料的简便合成方法

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-25 DOI: 10.1002/jssc.202400471

Qian Qin, Xiaolong Liu, Xun Wang, Lina Zhou, Huihui Wan, Qingxin Yin, Di Chen

{"title":"Facile Synthesis of Aptamer-Functionalized Polydopamine-Coated Magnetic Graphene Oxide Nanocomposites for Highly Efficient Purification of His-Tagged Proteins","authors":"Qian Qin, Xiaolong Liu, Xun Wang, Lina Zhou, Huihui Wan, Qingxin Yin, Di Chen","doi":"10.1002/jssc.202400471","DOIUrl":"https://doi.org/10.1002/jssc.202400471","url":null,"abstract":"<div>\u0000 \u0000 <p>Recombinant proteins hold significant importance in numerous disciplines. As the demand for expressing and purifying these proteins grows, the scientific community is in dire need of a simple yet versatile methodology that can efficiently purify these proteins. Aptamers as synthetic nucleic acid–based ligands with high affinity have shown promise in this regard, as they can capture targets through molecular recognition. In this study, novel aptamer-functionalized polydopamine-coated magnetic graphene oxide nanocomposites were facilely prepared, achieving an impressive average aptamer coverage density (45 nmol/mg). These nanocomposites exhibited a uniform structure and robust magnetic responsiveness. The findings indicated that they possess several advantages, such as rapid adsorption, substantial capacity (171.4 mg/g), and excellent reusability. Notably, due to the inherent properties of nucleic acids, the immobilized aptamer-magnetic beads can be utilized repeatedly with high purification efficiency. Finally, the nanocomposites were further employed to purify His-tagged proteins from actual samples. Remarkably, they were able to selectively and efficiently isolate His-tagged retinoid X receptor alpha protein from complex <i>Escherichia coli</i> lysate. The purified His-tagged retinoid X receptor alpha protein was analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. This confirmed the efficacy of developed nanocomposites, reinforcing their vast potential for purification of His-tagged recombinant proteins.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Characterization of a Soft Ionization by Chemical Reaction in Transfer Ion Source Hyphenated With Supercritical Fluid Chromatography-Tandem Mass Spectrometry 超临界流体色谱-串联质谱联用转移离子源中化学反应软电离的表征

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-25 DOI: 10.1002/jssc.202400500

Niray Bhakta, Alexander S. Kaplitz, Destini Black, Kevin A. Schug

{"title":"Characterization of a Soft Ionization by Chemical Reaction in Transfer Ion Source Hyphenated With Supercritical Fluid Chromatography-Tandem Mass Spectrometry","authors":"Niray Bhakta, Alexander S. Kaplitz, Destini Black, Kevin A. Schug","doi":"10.1002/jssc.202400500","DOIUrl":"https://doi.org/10.1002/jssc.202400500","url":null,"abstract":"<div>\u0000 \u0000 <p>A commercially available dielectric barrier discharge ionization (DBDI) source was tested with supercritical fluid chromatography-mass spectrometry (SFC-MS). The compound mixture investigated comprised caffeine, theobromine, theophylline, uracil, testosterone, and pyrene, diluted in methanol. Dynamic response ranges were evaluated with multiple injections at different concentrations. Precision studies demonstrated the robustness and sensitivity of the ionization source across a concentration range of 10–1000 ng/mL. Results from this experiment showed linear regression of 0.99 or greater for all analytes tested over the range with a relative standard deviation (RSD) of less than 10% down to 10 ng/mL for all analytes except theobromine, which had an RSD of less than 10% down to 25 ng/mL. Notably, this study marks the first investigation of sensitivity for coupling a commercial DBDI source with SFC; a limit of detection less than 1 ng/mL was achieved for all compounds. This study demonstrates chromatographic separation by SFC and MS analysis for compounds that ionize poorly using traditional atmospheric pressure ionization, such as polycyclic aromatic hydrocarbons. Combining SFC with the DBDI source opens promising avenues for analyzing compounds that were previously challenging to characterize with standard atmospheric pressure ionization techniques.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Advancements in Multiple-Step On-Line Preconcentration Techniques for Enhanced Sensitivity in Capillary Electrophoresis 提高毛细管电泳灵敏度的多步在线预浓缩技术的进展

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-20 DOI: 10.1002/jssc.202400519

Aemi Syazwani Abdul Keyon, NyukTing Ng, Michael Charles Breadmore

{"title":"Advancements in Multiple-Step On-Line Preconcentration Techniques for Enhanced Sensitivity in Capillary Electrophoresis","authors":"Aemi Syazwani Abdul Keyon, NyukTing Ng, Michael Charles Breadmore","doi":"10.1002/jssc.202400519","DOIUrl":"10.1002/jssc.202400519","url":null,"abstract":"<div>\u0000 \u0000 <p>Multiple-step on-line preconcentration, a combination of at least two stacking techniques has been developed to increase the sensitivity in capillary electrophoresis (CE) for analytes in various samples. It is usually conducted sequentially, or in some cases, synergistically, where different stacking modes occur simultaneously. Multiple-step techniques allow simultaneous preconcentration and separation of various kinds of analytes in different complex samples in a single CE run. This review aims to provide recent advances in multiple-step on-line preconcentration techniques in CE. We critically review technical papers published for the last 7 years up until July 2024, subsequently organized according to the combination of the main stacking techniques, that is, field amplification, large volume sample stacking, transient isotachophoresis, micelle to solvent or micelle to cyclodextrin stacking, and others. The procedures, fundamental mechanism, analytical figures of merits achieved, and their feasibility for complicated sample matrices are reviewed.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142273169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Thermo-Assisted Deep Eutectic Solvent Based on Dispersive Liquid-Liquid Microextraction for Preconcentration of Phthalate Esters in Water Samples and Determination by Gas Chromatography With Flame Ionization Detection 基于分散液-液微萃取技术的热助深共晶溶剂用于预富集水样中的邻苯二甲酸酯并通过火焰离子化检测气相色谱法进行测定

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-20 DOI: 10.1002/jssc.202300878

Khosrou Abdi, Maryam Ezoddin, Venous Novasari, Navid Lamei

{"title":"Thermo-Assisted Deep Eutectic Solvent Based on Dispersive Liquid-Liquid Microextraction for Preconcentration of Phthalate Esters in Water Samples and Determination by Gas Chromatography With Flame Ionization Detection","authors":"Khosrou Abdi, Maryam Ezoddin, Venous Novasari, Navid Lamei","doi":"10.1002/jssc.202300878","DOIUrl":"10.1002/jssc.202300878","url":null,"abstract":"<div>\u0000 \u0000 <p>A thermo-assisted deep eutectic solvent (DES) based on dispersive liquid-liquid microextraction followed by gas chromatography with flame ionization detection was developed for the analysis of five phthalate esters in different water samples. In the procedure involved, a DES composed of lidocaine, an amphiphilic amine, and oleic acid, was mixed with the sample assisted by ultrasound, and phase separation was achieved with increasing temperature. The heating of the extraction system induced the change of acid-base properties of the DES components. Thus, the formation of microdroplets of DES in the sample was provided, and two phases were separated. The structure of the upper hydrophobic layer was characterized by Fourier-transform infrared spectroscopy. Also, the amount of water in the DES phase was analyzed by mass spectrometer and Karl Fischer titration. Some critical variables on the extraction yield were assessed. The proposed method achieved 1.2–1.3 and 4.1–4.3 µg/L for limits of detection and limits of quantification, respectively. The intra-day and inter-day percentage relative standard deviations (<i>n</i> = 5) were determined to be in the range of 4.2–6.2% and 5.1–7.2%, respectively. Ultimately, this method analyzed the five phthalate esters in different water samples with high recoveries.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142273170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A Dual Membrane Electrodialytic Phosphoric Acid Eluent Generator for Ion Exclusion Chromatography—A Gas-Free Electrodialytic Phosphoric Acid Generator 用于离子排阻色谱法的双膜电解磷酸流体发生器--无气电解磷酸发生器

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-20 DOI: 10.1002/jssc.202400536

Xiaolu Sun, Jingwen Tang, Weiqing Chen, Feifang Zhang, Bingcheng Yang

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Boric Acid Functionalized Hypercrosslinked Polymers for Selective Extraction of Trace Catecholamines and Their Metabolites in Rat Serum 用于选择性萃取大鼠血清中痕量儿茶酚胺及其代谢物的硼酸功能化超交联聚合物

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-20 DOI: 10.1002/jssc.202400418

Zhiyu Zhang, Cheng Yang, Di Zhao, Yuanqing Zhao, Lixin Li, Zhonghua Li, Zhenqiang Zhang, Kai Hu

{"title":"Boric Acid Functionalized Hypercrosslinked Polymers for Selective Extraction of Trace Catecholamines and Their Metabolites in Rat Serum","authors":"Zhiyu Zhang, Cheng Yang, Di Zhao, Yuanqing Zhao, Lixin Li, Zhonghua Li, Zhenqiang Zhang, Kai Hu","doi":"10.1002/jssc.202400418","DOIUrl":"10.1002/jssc.202400418","url":null,"abstract":"<div>\u0000 \u0000 <p>Abnormal levels of catecholamine (CA) neurotransmitters and their metabolites in biological fluids can lead to various neurological disorders. Herein, a boric acid-functionalized hypercrosslinked polymer was prepared and utilized as a sorbent for the dispersive solid-phase extraction of CAs and their metabolites in rat serum. By combination with a high-performance liquid chromatography-fluorescence detector, the extraction parameters for the seven target analytes were optimized. Under the optimal extraction condition, the methodology for the quantitative analysis of CAs and their metabolites in rat serum samples was established. The limits of detection and limits of quantification were found to be in the ranges of 0.010–0.015 and 0.033–0.050 ng/mL, respectively. The results demonstrated satisfactory recoveries, with values ranging from 88.02% to 113.27%, accompanied by relative standard deviations within the range of 2.69%–9.59%. In addition, the method showed good anti-interference ability (matrix effect ranged from 2.64% to 18.07%). The developed method was validated for the determination of CAs and their metabolites in normal and Alzheimer's disease model rats’ serum, which proved the promising application of the method for CAs neurotransmitter analysis in biological samples.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142273171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Recent advances in liquid chromatography-tandem mass spectrometry for the detection of thyroid hormones and thyroglobulin in clinical samples: A review 液相色谱-串联质谱法检测临床样本中甲状腺激素和甲状腺球蛋白的最新进展：综述

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-18 DOI: 10.1002/jssc.202400466

Yuting Jin, Taiyu Zhai, Ying Wang, Jiuyan Li, Tingting Wang, Jing Huang

{"title":"Recent advances in liquid chromatography-tandem mass spectrometry for the detection of thyroid hormones and thyroglobulin in clinical samples: A review","authors":"Yuting Jin, Taiyu Zhai, Ying Wang, Jiuyan Li, Tingting Wang, Jing Huang","doi":"10.1002/jssc.202400466","DOIUrl":"https://doi.org/10.1002/jssc.202400466","url":null,"abstract":"<p>Thyroid hormones (THs), including triiodothyronine (T3), thyroxine (T4), and their metabolites, are essential for regulating development, growth, and energy metabolism. Thyroglobulin (Tg) produced by thyroid follicular cells acts as an essential substrate for TH synthesis. The combination of THs with Tg is a widely used serological laboratory test for thyroid function assessment. Early detection and timely intervention are significant for preventing and managing thyroid disease. In recent years, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has emerged as a powerful tool for the precise detection of small molecular analytes and steroid hormones in clinical practice as a result of its high sensitivity and specificity. While LC-MS/MS has been increasingly used for detecting THs and Tg recently, its application in clinical practice is still in its early stages. Recent advances in the assessment of thyroid metabolism using LC-MS/MS in clinical samples published during 2004–2023 were reviewed, with a special focus on the use of this technique for quantifying molecules involved in thyroid diseases.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142273000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-tube solid-phase microextraction of polycyclic aromatic hydrocarbons from refinery water samples using UiO-66/polyacrylonitrile electrospun nanofibers followed by high-performance liquid chromatography-ultraviolet detection 使用 UiO-66/ 聚丙烯腈电纺纳米纤维对炼油厂水样中的多环芳烃进行管内固相微萃取，然后进行高效液相色谱-紫外检测

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2024-09-14 DOI: 10.1002/jssc.202400296

Seyedeh Sara Nasrollahi, Yadollah Yamini

{"title":"In-tube solid-phase microextraction of polycyclic aromatic hydrocarbons from refinery water samples using UiO-66/polyacrylonitrile electrospun nanofibers followed by high-performance liquid chromatography-ultraviolet detection","authors":"Seyedeh Sara Nasrollahi, Yadollah Yamini","doi":"10.1002/jssc.202400296","DOIUrl":"10.1002/jssc.202400296","url":null,"abstract":"<p>A simple and quick fiber-in-tube solid-phase microextraction (FIT-SPME) was introduced for the extraction and determination of nine polycyclic aromatic hydrocarbons followed by a high-performance liquid chromatography-ultraviolet detector in refinery water samples. For this purpose, a water-resistant metal-organic framework with a high surface area called UiO-66 has been applied in the form of an electrospun coating on stainless steel wires. After that, all the fibers were packed in the lumen of a stainless-steel tube to make the extraction phase. Both one variable at a time and experimental design methods have been used to optimize effective parameters on FIT-SPME. Under optimum conditions, the method demonstrated good linearity between 0.5 and 1000.0 µg/L with a coefficient of determination greater than 0.9906. Furthermore, the limits of detection values ranged from 0.2 to 1.5 µg/L. The intra-day and inter-day relative standard deviations were < 8.4% and < 9.7%, respectively. Lastly, the proposed method was applied to extract and determine analytes in four refinery water samples as well as surface water containing high total dissolved solids, and well waters where satisfactory results have been obtained.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"47 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142233182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0