Journal of separation science最新文献_第5页

Chlorophylls and β-carotene in Spirulina platensis Dietary Supplements: Nano Liquid Chromatography Versus Diffuse Reflectance Spectroscopy for Establishing Composition, Quality Control, and Comparison of Commercial Products 螺旋藻膳食补充剂中的叶绿素和β-胡萝卜素：纳米液相色谱与漫反射光谱建立成分、质量控制和商业产品比较。

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-09-10 DOI: 10.1002/jssc.70260

C. Soto, R. Herráez-Hernández, P. Campíns-Falcó

{"title":"Chlorophylls and β-carotene in Spirulina platensis Dietary Supplements: Nano Liquid Chromatography Versus Diffuse Reflectance Spectroscopy for Establishing Composition, Quality Control, and Comparison of Commercial Products","authors":"C. Soto, R. Herráez-Hernández, P. Campíns-Falcó","doi":"10.1002/jssc.70260","DOIUrl":"10.1002/jssc.70260","url":null,"abstract":"<p>Spirulina is considered a superfood due to its chlorophylls. Two new methods for the determination of chlorophylls and β-carotene were developed here, one based on in-tube solid-phase microextraction (IT-SPME) coupled online to nanoliquid chromatography (nanoLC) with diode array detection (DAD), and the other on ultraviolet-visible diffuse reflectance spectroscopy (UV-vis DRS). A protocol to extract the pigments from spirulina was proposed using ethanol (1.5 mL). The aim is to provide fast and environmentally friendly techniques for both the extraction and measurement of pigments, generating little waste and low energy expenditure. IT-SPME-nanoLC-DAD and UV-vis DRS showed good linearity up to 300 or 600 µg/L and 50 mg/L, with instrumental detection limits between 10 and 50 µg/L and 2 and 5 mg/L, respectively. The % intraday and interday relative standard deviation were between 2 and 9. The HEXAGON tool is used for assessing greenness and sustainability for three scenarios: establishing composition, quality control, and comparison of seven dietary supplements</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70260","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green Synthetic Amide-Modified Hyper-Cross-Linked Adsorption Resin for Efficiently Separating Chlorogenic Acid From Eucommia ulmoides Extracts 绿色合成酰胺改性超交联吸附树脂高效分离杜仲提取物中的绿原酸

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-09-08 DOI: 10.1002/jssc.70256

Xuefang Chen, Zhijie Shen, Haifeng Chen, Hairong Chen, Tessafa Abrham Ashagrie, Hailong Li, Hairong Zhang, Lian Xiong, Xinde Chen

{"title":"Green Synthetic Amide-Modified Hyper-Cross-Linked Adsorption Resin for Efficiently Separating Chlorogenic Acid From Eucommia ulmoides Extracts","authors":"Xuefang Chen, Zhijie Shen, Haifeng Chen, Hairong Chen, Tessafa Abrham Ashagrie, Hailong Li, Hairong Zhang, Lian Xiong, Xinde Chen","doi":"10.1002/jssc.70256","DOIUrl":"10.1002/jssc.70256","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Eucommia ulmoides Oliver</i> leaf is rich in chlorogenic acid, which has antioxidant, antiviral, and anti-inflammatory activities. In this work, a new and green strategy for functional hyper-crosslinked adsorption resin based on Friedel–Crafts reaction of pendant vinyl groups in divinylbenzene with anhydrous ethanol and acrylamide grafting polymerization was developed, and the obtained HCREt-AM resin had excellent performance on chlorogenic acid separation from <i>Eucommia ulmoides Oliver</i> leaf extract. Adsorption isotherm and kinetics study showed the adsorption process fitted by Langmuir adsorption isotherm and pseudo-second-order kinetic equation. The dynamic saturated adsorption capacity of 88.0 mg·g<sup>−1</sup> was obtained, and chlorogenic acid content was raised from 7.91% to 48.27%. The pH-controlled ionization of chlorogenic acid determined the adsorption process, and π-π stacking, hydrophobic interactions, hydrogen bonding, and electrostatic interactions are the main adsorption mechanisms.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green Synthetic Amide-Modified Hyper-Cross-Linked Adsorption Resin for Efficiently Separating Chlorogenic Acid From Eucommia ulmoides Extracts 绿色合成酰胺改性超交联吸附树脂高效分离杜仲提取物中的绿原酸

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-09-08 DOI: 10.1002/jssc.70256

Xuefang Chen, Zhijie Shen, Haifeng Chen, Hairong Chen, Tessafa Abrham Ashagrie, Hailong Li, Hairong Zhang, Lian Xiong, Xinde Chen

{"title":"Green Synthetic Amide-Modified Hyper-Cross-Linked Adsorption Resin for Efficiently Separating Chlorogenic Acid From Eucommia ulmoides Extracts","authors":"Xuefang Chen, Zhijie Shen, Haifeng Chen, Hairong Chen, Tessafa Abrham Ashagrie, Hailong Li, Hairong Zhang, Lian Xiong, Xinde Chen","doi":"10.1002/jssc.70256","DOIUrl":"10.1002/jssc.70256","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Eucommia ulmoides Oliver</i> leaf is rich in chlorogenic acid, which has antioxidant, antiviral, and anti-inflammatory activities. In this work, a new and green strategy for functional hyper-crosslinked adsorption resin based on Friedel–Crafts reaction of pendant vinyl groups in divinylbenzene with anhydrous ethanol and acrylamide grafting polymerization was developed, and the obtained HCREt-AM resin had excellent performance on chlorogenic acid separation from <i>Eucommia ulmoides Oliver</i> leaf extract. Adsorption isotherm and kinetics study showed the adsorption process fitted by Langmuir adsorption isotherm and pseudo-second-order kinetic equation. The dynamic saturated adsorption capacity of 88.0 mg·g<sup>−1</sup> was obtained, and chlorogenic acid content was raised from 7.91% to 48.27%. The pH-controlled ionization of chlorogenic acid determined the adsorption process, and π-π stacking, hydrophobic interactions, hydrogen bonding, and electrostatic interactions are the main adsorption mechanisms.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enantioselective Properties of Neglected Drug Nifurtimox on Polysaccharide and Macrocyclic Glycopeptide Chiral Stationary Phases by Green HPLC Separation Methods 被忽视药物尼呋替莫对多糖和大环糖肽手性固定相的对映选择性

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-09-08 DOI: 10.1002/jssc.70258

Amanda Mohr, Érika Segala, Gustavo Machado das Neves, Vera Lucia Eifler-Lima, Martin Steppe

{"title":"Enantioselective Properties of Neglected Drug Nifurtimox on Polysaccharide and Macrocyclic Glycopeptide Chiral Stationary Phases by Green HPLC Separation Methods","authors":"Amanda Mohr, Érika Segala, Gustavo Machado das Neves, Vera Lucia Eifler-Lima, Martin Steppe","doi":"10.1002/jssc.70258","DOIUrl":"10.1002/jssc.70258","url":null,"abstract":"<p>Nifurtimox (NFX) is a chiral drug used for the treatment of Chagas Disease. Little attention has been paid to the enantioselective properties of chiral drugs used for neglected tropical diseases, highlighting the need for further studies in this area. In this work, the enantioselective properties of NFX were carefully investigated by HPLC using different chiral stationary phases (CSPs) and chromatographic modes. Two enantioseparation HPLC methods were successfully developed and validated. Polar ionic, polar organic, and RP modes were tested, and the polar organic mode proved to be suitable for the enantioseparation. Chromatographic resolution was achieved using the polysaccharide-based Chiralpak AD CSP and the macrocyclic glycopeptide-based Chirobiotic V CSP. Both methods employed ethanol as a mobile phase, contributing to greener analytical practices. The influence of the CSPs temperature on the retention of the enantiomers was investigated, and the analysis temperatures were set at 25°C. Thermodynamic parameters were also studied, and the enantioseparation process was found to be enthalpy-driven. Furthermore, molecular docking studies were conducted to identify the interactions of NFX with the CSPs constituents and to predict the elution order of the enantiomers. The elution order of the enantiomers was determined by comparing the experimental electronic circular dichroism (ECD) spectra with the theoretical ECD spectra, which aligned with the predictions made through molecular docking. The environmental impact of the developed methodologies was evaluated using the green metrics MoGAPI and AGREE, confirming their eco-friendly nature. The methods can be employed in future enantioselectivity studies and drug analysis.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70258","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enantioselective Properties of Neglected Drug Nifurtimox on Polysaccharide and Macrocyclic Glycopeptide Chiral Stationary Phases by Green HPLC Separation Methods 被忽视药物尼呋替莫对多糖和大环糖肽手性固定相的对映选择性

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-09-08 DOI: 10.1002/jssc.70258

Amanda Mohr, Érika Segala, Gustavo Machado das Neves, Vera Lucia Eifler-Lima, Martin Steppe

{"title":"Enantioselective Properties of Neglected Drug Nifurtimox on Polysaccharide and Macrocyclic Glycopeptide Chiral Stationary Phases by Green HPLC Separation Methods","authors":"Amanda Mohr, Érika Segala, Gustavo Machado das Neves, Vera Lucia Eifler-Lima, Martin Steppe","doi":"10.1002/jssc.70258","DOIUrl":"10.1002/jssc.70258","url":null,"abstract":"<p>Nifurtimox (NFX) is a chiral drug used for the treatment of Chagas Disease. Little attention has been paid to the enantioselective properties of chiral drugs used for neglected tropical diseases, highlighting the need for further studies in this area. In this work, the enantioselective properties of NFX were carefully investigated by HPLC using different chiral stationary phases (CSPs) and chromatographic modes. Two enantioseparation HPLC methods were successfully developed and validated. Polar ionic, polar organic, and RP modes were tested, and the polar organic mode proved to be suitable for the enantioseparation. Chromatographic resolution was achieved using the polysaccharide-based Chiralpak AD CSP and the macrocyclic glycopeptide-based Chirobiotic V CSP. Both methods employed ethanol as a mobile phase, contributing to greener analytical practices. The influence of the CSPs temperature on the retention of the enantiomers was investigated, and the analysis temperatures were set at 25°C. Thermodynamic parameters were also studied, and the enantioseparation process was found to be enthalpy-driven. Furthermore, molecular docking studies were conducted to identify the interactions of NFX with the CSPs constituents and to predict the elution order of the enantiomers. The elution order of the enantiomers was determined by comparing the experimental electronic circular dichroism (ECD) spectra with the theoretical ECD spectra, which aligned with the predictions made through molecular docking. The environmental impact of the developed methodologies was evaluated using the green metrics MoGAPI and AGREE, confirming their eco-friendly nature. The methods can be employed in future enantioselectivity studies and drug analysis.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70258","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolite Profiling of Guipi Pill Using UHPLC-Q-Orbitrap-HRMS and Determination of the Pharmacokinetics of its Major Prototype Components 桂皮丸代谢产物的UHPLC-Q-Orbitrap-HRMS分析及主要原型成分的药动学测定

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-09-03 DOI: 10.1002/jssc.70257

Huafang Bai, Hongjin Wang, Nannan Yuan, Hui Zhang, Qi Sun, Mengru Wang, Jinwei Gao

{"title":"Metabolite Profiling of Guipi Pill Using UHPLC-Q-Orbitrap-HRMS and Determination of the Pharmacokinetics of its Major Prototype Components","authors":"Huafang Bai, Hongjin Wang, Nannan Yuan, Hui Zhang, Qi Sun, Mengru Wang, Jinwei Gao","doi":"10.1002/jssc.70257","DOIUrl":"10.1002/jssc.70257","url":null,"abstract":"<div>\u0000 \u0000 <p>Guipi Pill (GPP), a classic traditional formula used to treat anxiety disorders, demonstrates significant pharmacological activity. However, research on its pharmacodynamic material basis remains limited. UHPLC-Q-Orbitrap-HRMS was used to systematically analyze the metabolites of GPP in the blood, urine, bile, and feces of rats after oral administration and elucidate its in vivo metabolic processes. The pharmacokinetic characteristics of the major prototype components were determined using UHPLC-MS/MS. A total of 106 compounds (48 prototype components and 58 metabolites) were identified in GPP, which included the following six structural classes: phenylpropanoids, flavonoids and their glycosides, terpenoids and their glycosides, volatile oils, organic acids, and acetylenic glycosides. Nine of the metabolites were presumed to be novel compounds, which were previously unreported. Metabolic pathways involved Phase I reactions (hydrolysis, methylation, oxidation) and Phase II reactions (glucuronidation or sulfation of Phase I metabolites). Pharmacokinetic analysis revealed flavonoid glycosides (e.g., liquiritin) to exhibit a short time to attain maximum concentration (<i>T</i><sub>max</sub>), short elimination half-life (<i>t</i><sub>1/2</sub>), and short mean residence time (MRT), consistent with their structural features, whereas terpenoids (e.g., costunolide, atractylenolide I, dehydrocostus lactone, glycyrrhetinic acid) showed slow absorption, slow elimination, and a long MRT. The volatile oil, ligustilide, demonstrated rapid absorption and a long <i>t</i><sub>1/2</sub>. This study systematically delineates the metabolic characteristics and pharmacokinetic profiles of the major constituents of GPP in rats, providing a scientific basis for the further elucidation of its pharmacodynamic material basis and mechanisms of metabolic regulation.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144929806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Commodity Crop Byproducts Into Carbon-Based Adsorbents for Flavonoid Recovery: A Review on Key Pyrolysis Parameters, Chemical Activation, and Adsorption Mechanisms 将商品作物副产品转化为碳基吸附剂用于类黄酮的回收：关键热解参数、化学活化和吸附机理综述

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-08-30 DOI: 10.1002/jssc.70252

Chandrika Visallane Chandiran, Mohamad Shazeli Che Zain

{"title":"Transforming Commodity Crop Byproducts Into Carbon-Based Adsorbents for Flavonoid Recovery: A Review on Key Pyrolysis Parameters, Chemical Activation, and Adsorption Mechanisms","authors":"Chandrika Visallane Chandiran, Mohamad Shazeli Che Zain","doi":"10.1002/jssc.70252","DOIUrl":"10.1002/jssc.70252","url":null,"abstract":"<div>\u0000 \u0000 <p>The development of carbon-based adsorbents for flavonoid purification has gained significant attention due to their high efficiency, sustainability, and cost-effectiveness. Derived from agricultural waste, such as fruit peels, husks, and stems, bio-adsorbents offer an eco-friendly approach to valorizing biomass. These materials are rich in carbon content and can be transformed into highly porous activated carbon through pyrolysis and chemical activation. Chemical activation methods, employing agents like phosphoric acid, zinc chloride, or potassium hydroxide, enhance the surface area, pore volume, and functional groups of the adsorbent, optimizing its adsorption capacity. Advanced carbon-based adsorption techniques leverage these properties to selectively bind flavonoids, benefiting from the high specificity and stability of the adsorbent. Adsorption mechanisms, including hydrogen bonding and van der Waals forces, play a crucial role in the effective capture of flavonoids. These processes are influenced by factors, such as surface chemistry, pore structure, and adsorbate properties, making carbon-based adsorbents a versatile and sustainable solution for purifying valuable bioactive compounds in pharmaceutical and nutraceutical industries.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Commodity Crop Byproducts Into Carbon-Based Adsorbents for Flavonoid Recovery: A Review on Key Pyrolysis Parameters, Chemical Activation, and Adsorption Mechanisms 将商品作物副产品转化为碳基吸附剂用于类黄酮的回收：关键热解参数、化学活化和吸附机理综述

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-08-30 DOI: 10.1002/jssc.70252

Chandrika Visallane Chandiran, Mohamad Shazeli Che Zain

{"title":"Transforming Commodity Crop Byproducts Into Carbon-Based Adsorbents for Flavonoid Recovery: A Review on Key Pyrolysis Parameters, Chemical Activation, and Adsorption Mechanisms","authors":"Chandrika Visallane Chandiran, Mohamad Shazeli Che Zain","doi":"10.1002/jssc.70252","DOIUrl":"10.1002/jssc.70252","url":null,"abstract":"<div>\u0000 \u0000 <p>The development of carbon-based adsorbents for flavonoid purification has gained significant attention due to their high efficiency, sustainability, and cost-effectiveness. Derived from agricultural waste, such as fruit peels, husks, and stems, bio-adsorbents offer an eco-friendly approach to valorizing biomass. These materials are rich in carbon content and can be transformed into highly porous activated carbon through pyrolysis and chemical activation. Chemical activation methods, employing agents like phosphoric acid, zinc chloride, or potassium hydroxide, enhance the surface area, pore volume, and functional groups of the adsorbent, optimizing its adsorption capacity. Advanced carbon-based adsorption techniques leverage these properties to selectively bind flavonoids, benefiting from the high specificity and stability of the adsorbent. Adsorption mechanisms, including hydrogen bonding and van der Waals forces, play a crucial role in the effective capture of flavonoids. These processes are influenced by factors, such as surface chemistry, pore structure, and adsorbate properties, making carbon-based adsorbents a versatile and sustainable solution for purifying valuable bioactive compounds in pharmaceutical and nutraceutical industries.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emulsifier-Free Emulsion Polymerization-Based Copper-Mediated Molecularly Imprinted Polymer for Vitamin B1 Extraction 基于无乳化剂乳液聚合的铜介导分子印迹聚合物用于维生素B1的提取

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-08-23 DOI: 10.1002/jssc.70255

Le Wu, Yumeng Liu, Ziying Zhang, Liang Pan, Xinzhu Dong, Shun Feng, Chungu Zhang

{"title":"Emulsifier-Free Emulsion Polymerization-Based Copper-Mediated Molecularly Imprinted Polymer for Vitamin B1 Extraction","authors":"Le Wu, Yumeng Liu, Ziying Zhang, Liang Pan, Xinzhu Dong, Shun Feng, Chungu Zhang","doi":"10.1002/jssc.70255","DOIUrl":"10.1002/jssc.70255","url":null,"abstract":"<div>\u0000 \u0000 <p>Here, we present a copper-mediated magnetic molecularly imprinted polymer (Cu-mMIP) as dispersive solid-phase extracting material (dSPE) for fast, selective, and specific extraction of a metabolic biomarker vitamin B1 (VB1) in complex biological matrices. With emulsifier-free emulsion polymerization using styrene and itaconic acid as functional co-monomers, Cu<sup>2+</sup> as central atom, and Fe<sub>3</sub>O<sub>4</sub> nanoparticles (NPs) as core, the resulted Cu-mMIP addresses VB1's structural challenges (conformational flexibility, hydrophilicity) while enabling rapid magnetic separation (< 10 s). The Cu-mMIP demonstrates exceptional specificity for VB1, achieving an imprinting factor of 5.63 and a maximum adsorption capacity of 48.75 mg/g, 2.36-fold higher than non-copper counterparts. Adsorption equilibrium is attained within 20 min, driven by chemisorption via pseudo-second-order kinetics. Competitive binding assays reveal twofold selectivity for VB1 over its structural analog thiamine pyrophosphate and negligible interference from other B vitamins (VB2, VB9, and VB12). When applied to simulated human plasma, the method achieved a detection limit of 9 ng/mL (<i>S</i>/<i>N</i> = 3) with recoveries of 84.3%–90.5% at three spiking levels (RSD < 5%, <i>n</i> = 3), effectively eliminating matrix interferences. This work establishes a scalable, high-efficiency platform for clinical nutrient analysis, combining molecular imprinting precision with metal coordination robustness, and advances separation science by addressing critical challenges in biomarker enrichment and high-throughput sample pretreatment.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emulsifier-Free Emulsion Polymerization-Based Copper-Mediated Molecularly Imprinted Polymer for Vitamin B1 Extraction 基于无乳化剂乳液聚合的铜介导分子印迹聚合物用于维生素B1的提取

IF 2.8 3区工程技术

Journal of separation science Pub Date : 2025-08-23 DOI: 10.1002/jssc.70255

Le Wu, Yumeng Liu, Ziying Zhang, Liang Pan, Xinzhu Dong, Shun Feng, Chungu Zhang

{"title":"Emulsifier-Free Emulsion Polymerization-Based Copper-Mediated Molecularly Imprinted Polymer for Vitamin B1 Extraction","authors":"Le Wu, Yumeng Liu, Ziying Zhang, Liang Pan, Xinzhu Dong, Shun Feng, Chungu Zhang","doi":"10.1002/jssc.70255","DOIUrl":"10.1002/jssc.70255","url":null,"abstract":"<div>\u0000 \u0000 <p>Here, we present a copper-mediated magnetic molecularly imprinted polymer (Cu-mMIP) as dispersive solid-phase extracting material (dSPE) for fast, selective, and specific extraction of a metabolic biomarker vitamin B1 (VB1) in complex biological matrices. With emulsifier-free emulsion polymerization using styrene and itaconic acid as functional co-monomers, Cu<sup>2+</sup> as central atom, and Fe<sub>3</sub>O<sub>4</sub> nanoparticles (NPs) as core, the resulted Cu-mMIP addresses VB1's structural challenges (conformational flexibility, hydrophilicity) while enabling rapid magnetic separation (< 10 s). The Cu-mMIP demonstrates exceptional specificity for VB1, achieving an imprinting factor of 5.63 and a maximum adsorption capacity of 48.75 mg/g, 2.36-fold higher than non-copper counterparts. Adsorption equilibrium is attained within 20 min, driven by chemisorption via pseudo-second-order kinetics. Competitive binding assays reveal twofold selectivity for VB1 over its structural analog thiamine pyrophosphate and negligible interference from other B vitamins (VB2, VB9, and VB12). When applied to simulated human plasma, the method achieved a detection limit of 9 ng/mL (<i>S</i>/<i>N</i> = 3) with recoveries of 84.3%–90.5% at three spiking levels (RSD < 5%, <i>n</i> = 3), effectively eliminating matrix interferences. This work establishes a scalable, high-efficiency platform for clinical nutrient analysis, combining molecular imprinting precision with metal coordination robustness, and advances separation science by addressing critical challenges in biomarker enrichment and high-throughput sample pretreatment.</p>\u0000 </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0