Journal of Phycology最新文献_第8页

Variability in spectral absorption within cryptophyte phycobiliprotein types 隐花植物藻体蛋白类型的光谱吸收差异。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-03-08 DOI: 10.1111/jpy.13439

Kristiaän A. Merritt, Tammi L. Richardson

{"title":"Variability in spectral absorption within cryptophyte phycobiliprotein types","authors":"Kristiaän A. Merritt, Tammi L. Richardson","doi":"10.1111/jpy.13439","DOIUrl":"10.1111/jpy.13439","url":null,"abstract":"Cryptophytes are known to vary widely in coloration among species. These differences in color arise primarily from the presence of phycobiliprotein accessory pigments. There are nine defined cryptophyte phycobiliprotein (Cr-PBP) types, named for their wavelength of maximal absorbance. Because Cr-PBP type has traditionally been regarded as a categorical trait, there is a paucity of information about how spectral absorption characteristics of Cr-PBPs vary among species. We investigated variability in primary and secondary peak absorbance wavelengths and full width at half max (FWHM) values of spectra of Cr-PBPs extracted from 75 cryptophyte strains (55 species) grown under full spectrum irradiance. We show that there may be substantial differences in spectral shapes within Cr-PBP types, with Cr-Phycoerythrin (Cr-PE) 545 showing the greatest variability with two, possibly three, subtypes, while Cr-PE 566 spectra were the least variable, with only ±1 nm of variance around the mean absorbance maximum of 565 nm. We provide additional criteria for classification in cases where the wavelength of maximum absorbance alone is not definitive. Variations in spectral characteristics among strains containing the same presumed Cr-PBP type may indicate differing chromophore composition and/or the presence of more than one Cr-PBP in a single cryptophyte species.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13439","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140059715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous 24-epibrassinolide (EBL) facilitates cell growth of Chlorella pyrenoidosa under high temperatures by enhancing the photosynthetic energy utilization and alleviating oxidative damage 外源 24-epibrassinolide (EBL) 通过提高光合作用能量利用率和减轻氧化损伤，促进高温条件下拟肾小球藻细胞的生长。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-03-07 DOI: 10.1111/jpy.13440

Fang Su, Yongfu Li

{"title":"Exogenous 24-epibrassinolide (EBL) facilitates cell growth of Chlorella pyrenoidosa under high temperatures by enhancing the photosynthetic energy utilization and alleviating oxidative damage","authors":"Fang Su, Yongfu Li","doi":"10.1111/jpy.13440","DOIUrl":"10.1111/jpy.13440","url":null,"abstract":"The microalga Chlorella pyrenoidosa is cultivated extensively for its constituents, which are of significant economic worth. Large-scale growth of C. pyrenoidosa in outdoor environments is subject to various stressors such as elevated temperature. The purpose of this study was to assess the protective effects of exogenous 24-epibrassinolide (EBL) on C. pyrenoidosa under high-temperature conditions. Compared to a temperature of 30°C, increasing the temperature to 43°C reduced the enzymatic capacity for carbon assimilation and resulted in the buildup of reactive oxygen species (ROS), thus reducing photosynthesis and proliferation. It was observed that exogenous EBL protected C. pyrenoidosa cells against high temperatures, with an optimal EBL concentration of 100 nM, resulting in enhanced capacity for photosynthetic carbon assimilation with a notable reduction in the imbalance between the absorption of light and energy used under high-temperature conditions. The addition of 100 nM EBL resulted in a 25.4% increase in cell density when exposed to elevated temperatures for 7 days. In addition, exogenous EBL reduced ROS production and increased the activities of critical antioxidant enzymes. This, in turn, mitigated heat-induced oxidative damage, resulting in advantageous outcomes in terms of cellular development and maintenance.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140059714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional responses to salinity-induced changes in cell wall morphology of the euryhaline diatom Pleurosira laevis 盐度诱导海水硅藻 Pleurosira laevis 细胞壁形态变化的转录反应。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-03-06 DOI: 10.1111/jpy.13437

Shiho Kamakura, Gust Bilcke, Shinya Sato

{"title":"Transcriptional responses to salinity-induced changes in cell wall morphology of the euryhaline diatom Pleurosira laevis","authors":"Shiho Kamakura, Gust Bilcke, Shinya Sato","doi":"10.1111/jpy.13437","DOIUrl":"10.1111/jpy.13437","url":null,"abstract":"Diatoms are unicellular algae with morphologically diverse silica cell walls, which are called frustules. The mechanism of frustule morphogenesis has attracted attention in biology and nanomaterials engineering. However, the genetic regulation of the morphology remains unclear. We therefore used transcriptome sequencing to search for genes involved in frustule morphology in the centric diatom Pleurosira laevis, which exhibits morphological plasticity between flat and domed valve faces in salinity 2 and 7, respectively. We observed differential expression of transposable elements (TEs) and transporters, likely due to osmotic response. Up-regulation of mechanosensitive ion channels and down-regulation of Ca2+-ATPases in cells with flat valves suggested that cytosolic Ca2+ levels were changed between the morphologies. Calcium signaling could be a mechanism for detecting osmotic pressure changes and triggering morphological shifts. We also observed an up-regulation of ARPC1 and annexin, involved in the regulation of actin filament dynamics known to affect frustule morphology, as well as the up-regulation of genes encoding frustule-related proteins such as BacSETs and frustulin. Taken together, we propose a model in which salinity-induced morphogenetic changes are driven by upstream responses, such as the regulation of cytosolic Ca2+ levels, and downstream responses, such as Ca2+-dependent regulation of actin dynamics and frustule-related proteins. This study highlights the sensitivity of euryhaline diatoms to environmental salinity and the role of active cellular processes in controlling gross valve morphology under different osmotic pressures.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13437","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140039617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rosetta gen. nov. (Chlorophyta): Resolving the identity of red snow algal rosettes Rosetta gen：解决红雪藻莲座的身份问题。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-03-04 DOI: 10.1111/jpy.13438

Casey B. Engstrom, Breanna B. Raymond, Joud Albeitshawish, Anastasia Bogdanovic, Lynne M. Quarmby

{"title":"Rosetta gen. nov. (Chlorophyta): Resolving the identity of red snow algal rosettes","authors":"Casey B. Engstrom, Breanna B. Raymond, Joud Albeitshawish, Anastasia Bogdanovic, Lynne M. Quarmby","doi":"10.1111/jpy.13438","DOIUrl":"10.1111/jpy.13438","url":null,"abstract":"Thick-walled rosette-like snow algae were long thought to be a life stage of various other species of snow algae. Rosette-like cells have not been cultured, but by manually isolating cells from 38 field samples in southern British Columbia, we assigned a variety of rosette morphologies to DNA sequence. Phylogenetic analysis of Rubisco large-subunit (rbcL) gene, ribosomal internal transcribed spacer 2 (ITS2) rRNA region, and 18S rRNA gene revealed that the rosette-like cells form a new clade within the phylogroup Chloromonadinia. Based on these data, we designate a new genus, Rosetta, which comprises five novel species: R. castellata, R. floranivea, R. stellaria, R. rubriterra, and R. papavera. In a survey of 762 snow samples from British Columbia, we observed R. floranivea exclusively on snow overlying high-elevation glaciers, whereas R. castellata was observed at lower elevations, near the tree line. The other three species were rarely observed. Spherical red cells enveloped in a thin translucent sac were conspecific with Rosetta, possibly a developmental stage. These results highlight the unexplored diversity among snow algae and emphasize the utility of single-cell isolation to advance the centuries-old problem of disentangling life stages and cryptic species.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140028275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The chloroplast and mitochondrial genomes of two Gomphonema parvulum (Bacillariophyta) environmental isolates from South Carolina (United States) and Virginia (United States) 来自南卡罗来纳州（美国）和弗吉尼亚州（美国）的两个 Gomphonema parvulum（Bacillariophyta）环境分离物的叶绿体和线粒体基因组。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-03-03 DOI: 10.1111/jpy.13415

A. Bruce Cahoon, Katherine M. Johnson, Sydney M. Brown, Kalina M. Manoylov, James A. Nienow

{"title":"The chloroplast and mitochondrial genomes of two Gomphonema parvulum (Bacillariophyta) environmental isolates from South Carolina (United States) and Virginia (United States)","authors":"A. Bruce Cahoon, Katherine M. Johnson, Sydney M. Brown, Kalina M. Manoylov, James A. Nienow","doi":"10.1111/jpy.13415","DOIUrl":"10.1111/jpy.13415","url":null,"abstract":"Gomphonema parvulum is a cosmopolitan freshwater diatom that is used as an indicator in water quality biomonitoring. In this study, we report the culturing of two geographically separated isolates from southeastern North America, their morphology, and the sequencing and assembly of their mitochondrial and chloroplast genomes. Morphologically, both strains fit G. parvulum sensu lato, but the frustules from a protected habitat in South Carolina were smaller than those cited in the historic data of this species from the same location as well as a second culture from Virginia. Phylogenetic analyses using the rbcL gene placed both within a clade with G. parvulum. Genetic markers, including full chloroplast and mitochondrial genomes and the nuclear small subunit rRNA gene region were assembled from each isolate. The organellar genomes of the two strains varied slightly in size due to small differences in intergenic regions with chloroplast genomes of 121,035 bp and 121,482 bp and mitochondrial genomes of 34,639 bp and 34,654 bp. The intraspecific pairwise identities of the chloroplast and mitochondrial genomes of these two isolates were 97.9% and 95.4%, respectively. Multigene phylogenetic analysis demonstrated a close relationship between G. parvulum, Gomphoneis minuta, and Didymosphenia geminata.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13415","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The endemic kelp Lessonia corrugata is being pushed above its thermal limits in an ocean warming hotspot 在海洋变暖的热点地区，当地特有的海藻 Lessonia corrugata 正被推到热极限之上。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-03-01 DOI: 10.1111/jpy.13434

Cody James, Cayne Layton, Catriona L. Hurd, Damon Britton

{"title":"The endemic kelp Lessonia corrugata is being pushed above its thermal limits in an ocean warming hotspot","authors":"Cody James, Cayne Layton, Catriona L. Hurd, Damon Britton","doi":"10.1111/jpy.13434","DOIUrl":"10.1111/jpy.13434","url":null,"abstract":"Kelps are in global decline due to climate change, which includes ocean warming. To identify vulnerable species, we need to identify their tolerances to increasing temperatures and determine whether tolerances are altered by co-occurring drivers such as inorganic nutrient levels. This is particularly important for those species with restricted distributions, which may already be experiencing thermal stress. To identify thermal tolerance of the range-restricted kelp Lessonia corrugata, we conducted a laboratory experiment on juvenile sporophytes to measure performance (growth, photosynthesis) across its thermal range (4–22°C). We determined the upper thermal limit for growth and photosynthesis to be ~22–23°C, with a thermal optimum of ~16°C. To determine if elevated inorganic nitrogen availability could enhance thermal tolerance, we compared the performance of juveniles under low (4.5 μmol · d−1) and high (90 μmol · d−1) nitrate conditions at and above the thermal optimum (16–23.5°C). Nitrate enrichment did not enhance thermal performance at temperatures above the optimum but did lead to elevated growth rates at the thermal optimum. Our results indicate L. corrugata is likely to be extremely susceptible to moderate ocean warming and marine heatwaves. Peak sea surface temperatures during summer in eastern and northeastern Tasmania can reach up to 20–21°C, and climate projections suggest that L. corrugata's thermal limit will be regularly exceeded by 2050 as southeastern Australia is a global ocean-warming hotspot. By identifying the upper thermal limit of L. corrugata, we have taken a critical step in predicting the future of the species in a warming climate.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13434","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139996553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tropical bloom-forming mesoalgae Cladophoropsis sp. and Laurencia sp.—responses to ammonium enrichment and a simulated heatwave 热带开花中生藻类 Cladophoropsis sp.

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-02-25 DOI: 10.1111/jpy.13435

Anna Fricke, Felix Bast, Agustín Moreira-Saporiti, Giovanni Martins Bussanello, Flower E. Msuya, Mirta Teichberg

{"title":"Tropical bloom-forming mesoalgae Cladophoropsis sp. and Laurencia sp.—responses to ammonium enrichment and a simulated heatwave","authors":"Anna Fricke, Felix Bast, Agustín Moreira-Saporiti, Giovanni Martins Bussanello, Flower E. Msuya, Mirta Teichberg","doi":"10.1111/jpy.13435","DOIUrl":"10.1111/jpy.13435","url":null,"abstract":"Algal blooms are increasing worldwide, driven by elevated nutrient inputs. However, it is still unknown how tropical benthic algae will respond to heatwaves, which are expected to be more frequent under global warming. In the present study, a multifactorial experiment was carried out to investigate the potential synergistic effects of increased ammonium inputs (25 μM, control at 2.5 μM) and a heatwave (31°C, control at 25°C) on the growth and physiology (e.g., ammonium uptake, nutrient assimilation, photosynthetic performance, and pigment concentrations) of two bloom-forming algal species, Cladophoropsis sp. and Laurencia sp. Both algae positively responded to elevated ammonium concentrations with higher growth and chlorophyll a and lutein concentrations. Increased temperature was generally a less important driver, interacting with elevated ammonium by decreasing the algaes' %N content and N:P ratios. Interestingly, this stress response was not captured by the photosynthetic yield (Fv/Fm) nor by the carbon assimilation (%C), which increased for both algae at higher temperatures. The negative effects of higher temperature were, however, buffered by nutrient inputs, showing an antagonistic response in the combined treatment for the concentration of VAZ (violaxanthin, antheraxanthin, zeaxanthin) and thalli growth. Ammonium uptake was initially higher for Cladophoropsis sp. and increased for Laurencia sp. over experimental time, showing an acclimation capacity even in a short time interval. This experiment shows that both algae benefited from increased ammonium pulses and were able to overcome the otherwise detrimental stress of increasingly emerging temperature anomalies, which provide them a strong competitive advantage and might support their further expansions in tropical marine systems.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13435","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139944234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecophysiological and genomic approaches to cyanobacterial hardening for restoration 蓝藻硬化修复的生态生理学和基因组学方法。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-02-19 DOI: 10.1111/jpy.13436

Beatriz Roncero-Ramos, Valentina Savaglia, Benoit Durieu, Isabelle Van de Vreken, Aurore Richel, Annick Wilmotte

{"title":"Ecophysiological and genomic approaches to cyanobacterial hardening for restoration","authors":"Beatriz Roncero-Ramos, Valentina Savaglia, Benoit Durieu, Isabelle Van de Vreken, Aurore Richel, Annick Wilmotte","doi":"10.1111/jpy.13436","DOIUrl":"10.1111/jpy.13436","url":null,"abstract":"Cyanobacteria inhabit extreme environments, including drylands, providing multiple benefits to the ecosystem. Soil degradation in warm drylands is increasing due to land use intensification. Restoration methods adapted to the extreme stress in drylands are being developed, such as cyanobacteria inoculation to recover biocrusts. For this type of restoration method to be a success, it is crucial to optimize the survival of inoculated cyanobacteria in the field. One strategy is to harden them to be acclimated to stressful conditions after laboratory culturing. Here, we analyzed the genome and ecophysiological response to osmotic desiccation and UVR stresses of an Antarctic cyanobacterium, Stenomitos frigidus ULC029, which is closely related to other cyanobacteria from warm and cold dryland soils. Chlorophyll a concentrations showed that preculturing ULC029 under moderate osmotic stress improved its survival during an assay of desiccation plus rehydration under UVR. Additionally, its sequential exposure to these stress factors increased the production of exopolysaccharides, carotenoids, and scytonemin. Desiccation, but not osmotic stress, increased the concentrations of the osmoprotectants trehalose and sucrose. However, osmotic stress might induce the production of other osmoprotectants, for which the complete pathways were observed in the ULC029 genome. In total, 140 genes known to be involved in stress resistance were annotated. Here, we confirm that the sequential application of moderate osmotic stress and dehydration could improve cyanobacterial hardening for soil restoration by inducing several resistance mechanisms. We provide a high-quality genome of ULC029 and a description of the main resistance mechanisms (i.e., production of exopolysaccharides, osmoprotectants, chlorophyll, and carotenoids; DNA repair; and oxidative stress protection).","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139900087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the track of unknown algae 在未知藻类的轨道上

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-02-16 DOI: 10.1111/jpy.13428

Christine Maggs

引用次数: 0

A new combined measurement of single cell periplasmic oxygen and carbonate chemistry revealed the rule enforcing diatom adaptation of seawater bicarbonate utilization 一种新的单细胞周质氧和碳酸盐化学综合测量方法揭示了硅藻适应海水碳酸氢盐利用的规则。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-02-16 DOI: 10.1111/jpy.13429

Yusuke Matsuda

引用次数: 0