Journal of Phycology最新文献

{"title":"Optimization of ultrasound-assisted extraction of phenolic compounds from Sargassum carpophyllum using response surface methodology","authors":"Xin Liu,&nbsp;Qunjian Yin,&nbsp;Xuyang Chen,&nbsp;Enge Xie,&nbsp;Feng Jiang,&nbsp;Ying Liu","doi":"10.1111/jpy.70175","DOIUrl":"10.1111/jpy.70175","url":null,"abstract":"<p><i>Sargassum</i> spp. has been used in traditional Chinese medicine for nearly 2000 years to treat a variety of diseases due to its pharmacologically active components such as phenolics. In this work, response surface methodology (RSM) was employed to optimize the ultrasound-assisted extraction (UAE) procedures to obtain phenolic compounds from <i>Sargassum carpophyllum</i>, a brown alga widely growing on the coastline of Asian countries. The effects of four extraction variables including ethanol concentration (EtOH%) in the solvent, liquid–solid ratio, ultrasonic power, and duty cycle ratio (DCR) were evaluated using a central composite design (CCD), with the total phenol content (TPC) and antioxidant activity as the two responses. Two quadratic regression models were generated, and the optimum extraction conditions for the highest TPC and antioxidant activity were 50% ethanol, a 40 mL · g⁻¹ of liquid–solid ratio, 260 W, and a 5/6 DCR. The corresponding predicted TPC and antioxidant activity were 2.884 mg phloroglucinol (PHG) · g⁻¹ algae and 3.188 mg Trolox · g⁻¹ algae, respectively, which were in close agreement with the experimental validation values of 2.907 mg PHG · g⁻¹ algae and 3.267 mg Trolox · g⁻¹ algae, respectively. The results suggested that the two regression models could sufficiently describe the UAE procedure and optimize the TPC and antioxidant activity. Results of the Fourier Transform Infrared Spectrometer (FTIR) spectra revealed that phenolic compounds were the major composition of the optimized extract. This work provides essential knowledge in phenolic compounds extraction from <i>S. carpophyllum</i> through mathematical modeling and could be a useful reference for developing upscale UAE procedures.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"855-867"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147963969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of Gracilaria vermiculophylla invasion along the east coast of the United States—Current knowledge and future directions","authors":"Leah M. Nelson,&nbsp;Sophie J. McCoy","doi":"10.1111/jpy.70164","DOIUrl":"10.1111/jpy.70164","url":null,"abstract":"<p><i>Gracilaria vermiculophylla</i>, a red macroalga native to the Northwest Pacific, is now widespread in coastal estuarine ecosystems along the East Coast of the United States. Its broad environmental tolerance, high capacity for asexual reproduction via fragmentation, and ability to alter habitat structure have allowed it to persist and thrive, often outcompeting native macrophytes. This review synthesizes 48 studies on <i>G. vermiculophylla</i> from the East Coast of the United States, categorizing them into nine research areas: <i>(1) Habitat Formation and Alteration; (2) Trophic Dynamics and Grazer Interactions; (3) Impacts on Nutrient Cycling and Biogeochemical Processes; (4) Microbial and Microalgal Interactions; (5) Responses to Climate Change; (6) Population Genetics; (7) Chemical Ecology; (8) Economic Impacts; and (9) Restoration and Management Implications</i>. Although ecological and physiological studies dominate the literature, applied research on management, climate change impacts, and socio-economic outcomes remains limited. Addressing these knowledge gaps will require interdisciplinary approaches that link ecological, social, and economic dimensions to better develop effective management strategies. Effective management of <i>G. vermiculophylla</i> on the U.S. East Coast will require adaptive, context-dependent strategies that account for local habitat conditions, reproductive mode, and trade-offs between localized functions and the loss of native ecosystem services.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"757-780"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A unique sessile loricate euglenid, Lepocinclis loricata sp. nov. (Euglenophyta, Phacaceae), from South Africa: Evolutionary implications","authors":"Bożena Zakryś,&nbsp;Sanet Janse van Vuuren,&nbsp;Aleksandra Kluczek,&nbsp;Maja Łukomska-Kowalczyk","doi":"10.1111/jpy.70163","DOIUrl":"10.1111/jpy.70163","url":null,"abstract":"<p>In this paper, we describe <i>Lepocinclis loricata</i> sp. nov. (Phacaceae), a sessile loricate euglenid that differs from all known loricate taxa by its unique combination of the presence of a lorica, monad morphology, sessile habit, and phylogenetic position within the Phacaceae. Populations of this species inhabit two turbid, silt-rich waterbodies, namely an artificial impoundment in the Western Cape Province and a natural, small river in the Free State Province, South Africa. The cylindrical, transparent loricae of <i>L. loricata</i> sp. nov. are attached to fine clay and silt particles suspended in the water column. Each lorica encloses a rigid, spindle-shaped monad containing numerous chloroplasts without pyrenoids and two large, rod-like paramylon grains. Phylogenetic analyses place <i>L. loricata</i> sp. nov. within the <i>Lepocinclis</i> clade, representing a loricate euglenid identified in the family Phacaceae. This discovery provides new insight into the diversification and adaptive evolution of photosynthetic euglenids and the independent emergence of sessility within the group.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"846-854"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein acetylation regulates the wound-induced asexual spore formation in Pyropia yezoensis","authors":"Zehao Zhang,&nbsp;Wuxin You,&nbsp;Xiaowei Guan,&nbsp;Fugeng Tang,&nbsp;Ansgar Poetsch,&nbsp;Dongmei Wang","doi":"10.1111/jpy.70171","DOIUrl":"10.1111/jpy.70171","url":null,"abstract":"<p>Lysine acetylation (LysAc) of proteins plays critical regulatory roles in a wide range of biological processes in both prokaryotes and eukaryotes. However, characteristics of LysAc and related proteins in red algae have not been investigated. In a marine aquaculture species <i>Pyropia yezoensis</i>, thallus cells form and release asexual spores under wound stress, and this process provides an essential source of seedlings in aquaculture. In order to elucidate the underlying regulatory mechanisms during spore formation, we performed a global acetylproteome analysis in both intact and wounded thalli. A total of 4647 LysAc sites were identified on 1398 proteins. Among them, 361 proteins exhibited differential acetylations (DAPs) at the time of sporangia formation and 382 at the time of spore maturation. Functional classification of all the DAPs revealed that they were primarily associated with central metabolic pathways, highlighting the importance of lysine acetylation in asexual spore formation. Particularly, &gt;30 proteins related to photosynthesis and carbon fixation showed coordinated decline, consistent with the repressed photosynthetic efficiency after wounding. Starch was accumulated and the LysAc on the catalytic domain of starch synthase significantly increased during spore formation. Proteins related to cytoskeleton remodeling also had variations in LysAc, aligning with the observed depolymerization of microfilaments in spores. Our study provides not only fundamental information in protein acetylation in <i>Pyropia</i> as a prominent example for red seaweeds but also valuable insights on the post-translational regulation in wound-induced spore formation.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"1002-1015"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecophysiological study of green microalgae isolated from the grit crust of the Atacama Desert","authors":"K. Glaser,&nbsp;F. Kuschel,&nbsp;L. R. Prelle,&nbsp;P. Jung,&nbsp;U. Karsten","doi":"10.1111/jpy.70182","DOIUrl":"10.1111/jpy.70182","url":null,"abstract":"<p>Terrestrial algae play a critical, yet often overlooked, role in the functioning of biological soil crusts (biocrusts), which are considered microecosystems, particularly in extreme environments. This study investigated the ecophysiological traits of green algae isolated from the grit crust—a unique biocrust type reported a few years ago in the coastal Atacama Desert (National Park Pan de Azúcar), Chile. We assessed photosynthetic performance, temperature tolerance, and desiccation recovery in four strains: <i>Pseudochlorella signiensis</i> strain C11, <i>Lobosphaera incisa</i> strain LC2, and two strains of <i>Klebsormidium elegans</i>, C12 and C14. The results revealed significant interspecific variation, with <i>P. signiensis</i> exhibiting the highest photosynthetic capacity and both <i>Klebsormidium</i> strains demonstrating efficient light utilization. Desiccation recovery at very low relative air humidity was limited across all species, potentially reflecting the influence of frequent fog events in the coastal region of the Atacama, which result in a moderate air humidity. This study fills a critical knowledge gap regarding terrestrial algal ecology in South America and highlights the physiological traits enabling algal survival in one of the world's most extreme environments, providing insights into the resilience of biocrust communities in the face of environmental change.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"1031-1043"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70182","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dominance and toxicity without lethality: Exploring biomass, cyanometabolites, and Daphnia responses across Cyanobacterial strains","authors":"Łukasz Wejnerowski,&nbsp;Tamara Dulić,&nbsp;Sultana Akter,&nbsp;Michał Rybak,&nbsp;Agnieszka Piasecka,&nbsp;Jakub Szymkowiak,&nbsp;Oskar Kamiński,&nbsp;Anna Czerepska,&nbsp;Filip Pniewski,&nbsp;Zorica Svirčev,&nbsp;Ewa Poniecka,&nbsp;Krzysztof Zawierucha,&nbsp;Katarzyna Taylor,&nbsp;Marcin Krzysztof Dziuba,&nbsp;Jussi Meriluoto","doi":"10.1111/jpy.70173","DOIUrl":"10.1111/jpy.70173","url":null,"abstract":"<p>Cyanobacteria are cosmopolitan, important components of biomass with a remarkable ability to synthesize a diverse array of bioactive compounds, some of which may have toxic effects on other organisms and ecosystems. In this study, filamentous cyanobacterial strains isolated predominantly from temperate European freshwaters (<i>Aphanizomenon</i>, <i>Chrysosporum</i>, <i>Cuspidothrix</i>, <i>Dolichospermum</i>, <i>Planktothrix</i>, <i>Raphidiopsis</i>), with a subset from Arctic waters (<i>Microcoleus</i>, <i>Phormidesmis</i>), were qualitatively screened for commonly studied cyanometabolites, using immunoassays and chromatographic techniques. We also assessed the effects of culture extracts and filtrates on the survival of <i>Daphnia magna</i> and <i>Daphnia pulicaria</i>. The results indicated a broad capacity among tested strains to produce toxic and bioactive compounds. Specifically, we detected anabaenopeptins, anatoxin-a, cylindrospermopsin, microcystins, and saxitoxins. Although numerous strains showed the potential to produce a variety of toxic and bioactive compounds and reached high biomass after 40 days of incubation, their extracts and filtrates frequently had negligible negative effects on the survival of <i>Daphnia</i>. In contrast, significant lethality was observed in response to planktic <i>Limnothrix</i> sp. and glacial <i>Microcoelus autumnalis</i> that tested negative for the studied compounds, as well as for an <i>Aphanizomenon klebahnii</i> strain in which only anabaenopeptins were detected. We also observed a positive relationship between the biomass used to prepare cyanobacterial extracts and <i>Daphnia</i> survival, and this result raises the question of whether there is a trade-off between the growth rates of strains and their toxicities. Our study extends the current knowledge of the geographical distribution of toxic cyanobacteria and suggests that some of them may also produce other, yet unidentified toxic compounds.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"801-817"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interaction between PII and NAGK regulates arginine biosynthesis in the green microalga Haematococcus pluvialis","authors":"Ruijuan Ma,&nbsp;Ziyue Chen,&nbsp;Junjie Liu,&nbsp;Xing Meng,&nbsp;Xinyi Tao,&nbsp;Yucheng Chen,&nbsp;Chunxiao Zhang,&nbsp;Ling Wang,&nbsp;Kangle Lu,&nbsp;Xueshan Li,&nbsp;Kai Song,&nbsp;Jianfeng Chen,&nbsp;Youping Xie","doi":"10.1111/jpy.70165","DOIUrl":"10.1111/jpy.70165","url":null,"abstract":"<p>PII protein is widely acknowledged to regulate intracellular nitrogen and carbon metabolism by interacting with several crucial proteins. N-acetyl-L-glutamate kinase (NAGK), a rate-limiting enzyme for arginine biosynthesis, is regarded as a potential target of PII protein. Nevertheless, the regulatory function remains ambiguous in green algae and has not been investigated in <i>Haematococcus pluvialis</i>. In this study, the NAGK enzyme and PII protein of <i>H. pluvialis</i> (designated as HpNAGK and HpPII, respectively) and their interaction relationships were characterized. The results indicated that HpNAGK showed high similarity with the same enzyme in the green algae. A subcellular localization assay indicated that both HpPII and HpNAGK were located in the chloroplasts. Yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays distinctly verified the interaction between HpPII and HpNAGK, which occurs in the chloroplasts. The structure of the HpPII-HpNAGK complex was predicted through docking analysis. Moreover, the HpNAGK activity was significantly enhanced by HpPII in the presence of glutamine in vitro. Under nitrogen starvation, HpNAGK activity declined in vivo, concomitant with a reduction in arginine accumulation. The regulatory function of HpPII on HpNAGK activity aligned with that in <i>Chlamydomonas reinhardtii</i> but differed from that in <i>Dunaliella salina</i>, suggesting species specificity among green algae. These findings provide insights into the regulatory function of PII protein in green algae and help to unveil the response mechanisms of <i>H. pluvialis</i> to different nitrogen statuses.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"868-882"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147717068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling coralline diversity of mesophotic rhodoliths in subtropical Japan, including new species of Sporolithon, emended genera Orientalilithon and Roseolithon (Corallinophycidae, Rhodophyta)","authors":"Min-Khant-Kyaw,&nbsp;Masasuke Baba,&nbsp;Ryuta Terada,&nbsp;Aki Kato","doi":"10.1111/jpy.70176","DOIUrl":"10.1111/jpy.70176","url":null,"abstract":"<p>The mesophotic zone off Tanegashima Island (approximately 30–40 m deep) is a marine macroalgal hotspot in subtropical Japan; however, the taxonomic diversity of calcifying red algal community remains poorly understood. Our phylogenetic analyses based on <i>psb</i>A, <i>rbc</i>L, and COI-5P gene markers disclosed the presence of at least 12 rhodolith-forming non-geniculate coralline algae from five genera across three orders. The confirmation of previously described <i>Lithophyllum neo</i>-<i>okamurae</i>, <i>L</i>. <i>okamurae</i> (Corallinales), and <i>Orientalilithon confluens</i> (Hapalidiales) herein extended their vertical distributional range. The remaining nine species represent novelty, with four newly described and five unnamed taxa: <i>Crustaphytum</i> sp., <i>Roseolithon aggregatum</i> sp. nov., <i>R. sparsituberculatum</i> sp. nov., <i>Orientalilithon compactum</i> sp. nov., <i>Orientalilithon</i> sp. 2, Hapalidiales sp. (Hapalidiales), <i>Sporolithon variotuberculatum</i> sp. nov., <i>Sporolithon</i> sp. 1, and <i>Sporolithon</i> sp. 2 (Sporolithales). <i>Roseolithon aggregatum</i> barely differs from <i>R. sparsituberculatum</i> by external growth form and length of protuberances. These two mesophotic species can be separated from the shallow-water <i>R</i>. <i>littorale</i> and <i>R</i>. <i>sabulosum</i> (1–6 m depth) in Japan based on depth-stratified distribution. <i>Orientalilithon compactum</i> and <i>O</i>. <i>confluens</i> can, at present, be identified by DNA sequences and to some degree by spatial distribution. <i>Sporolithon variotuberculatum</i> is a genetically verified Japanese species of <i>Sporolithon</i> and appears to be related to <i>S</i>. <i>molle</i> from Egypt. They morpho-anatomically differed by the number of cells in paraphyses, the diameter of sporangial pore openings, and the size of stalk cells subtending tetra-/bisporangia. This study underscores the remarkable species diversity of Japanese rhodoliths in subtropical mesophotic ecosystems that deserve further investigation.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"1044-1070"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70176","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147930650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How do I publish the name of a new species of algae? A basic guide","authors":"Michael D. Guiry","doi":"10.1111/jpy.70172","DOIUrl":"10.1111/jpy.70172","url":null,"abstract":"<p>A basic guide to the requirements of the International Code of Nomenclature for algae, fungi, and plants for the publication of a valid name of new species (and infraspecific taxa) of living algae is provided. It is intended to aid phycologists so that an accurate inventory of the world's algal diversity is achieved. A checklist of actions necessary when preparing for publication of algae names is included.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"749-756"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70172","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to 'Assessment of rhodolith-forming species diversity in British Columbia uncovers novel cryptic diversity in the genera Boreolithothamnion and Rhodolithia gen. nov. (Florideophyceae, Rhodophyta) and the occurrence of hybrid rhodoliths'.","authors":"","doi":"10.1111/jpy.70146","DOIUrl":"10.1111/jpy.70146","url":null,"abstract":"","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":"1124"},"PeriodicalIF":4.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147369316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}