Journal of Phycology最新文献_第10页

Characterization of polyphosphate dynamics in the widespread freshwater diatom Achnanthidium minutissimum under varying phosphorus supplies 不同磷供应条件下广泛分布的淡水硅藻 Achnanthidium minutissimum 的多磷酸盐动力学特征

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2024-01-01 DOI: 10.1111/jpy.13423

Adrien Lapointe, Mustafa Kocademir, Paavo Bergman, Imaiyan Chitra Ragupathy, Michael Laumann, Graham J. C. Underwood, Andreas Zumbusch, Dieter Spiteller, Peter G. Kroth

{"title":"Characterization of polyphosphate dynamics in the widespread freshwater diatom Achnanthidium minutissimum under varying phosphorus supplies","authors":"Adrien Lapointe, Mustafa Kocademir, Paavo Bergman, Imaiyan Chitra Ragupathy, Michael Laumann, Graham J. C. Underwood, Andreas Zumbusch, Dieter Spiteller, Peter G. Kroth","doi":"10.1111/jpy.13423","DOIUrl":"10.1111/jpy.13423","url":null,"abstract":"Polyphosphates (polyP) are ubiquitous biomolecules that play a multitude of physiological roles in many cells. We have studied the presence and role of polyP in a unicellular alga, the freshwater diatom Achnanthidium minutissimum. This diatom stores up to 2.0 pg·cell−1 of polyP, with chain lengths ranging from 130 to 500 inorganic phosphate units (Pi). We applied energy dispersive X-ray spectroscopy, Raman/fluorescence microscopy, and biochemical assays to localize and characterize the intracellular polyP granules that were present in large apical vacuoles. We investigated the fate of polyP in axenic A. minutissimum cells grown under phosphorus (P), replete (P(+)), or P deplete (P(−)) cultivation conditions and observed that in the absence of exogenous P, A. minutissimum rapidly utilizes their internal polyP reserves, maintaining their intrinsic growth rates for up to 8 days. PolyP-depleted A. minutissimum cells rapidly took up exogenous P a few hours after Pi resupply and generated polyP three times faster than cells that were not initially subjected to P limitation. Accordingly, we propose that A. minutissimum deploys a succession of acclimation strategies regarding polyP dynamics where the production or consumption of polyP plays a central role in the homeostasis of the diatom.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13423","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct regulation of two flagella by calcium during chemotaxis of male gametes in the brown alga Mutimo cylindricus (Cutleriaceae, Tilopteridales) 褐藻 Mutimo cylindricus（Cutleriaceae, Tilopteridales）雄性配子趋化过程中钙质对两条鞭毛的不同调节作用

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2023-12-30 DOI: 10.1111/jpy.13422

Nana Kinoshita-Terauchi, Kogiku Shiba, Taiki Umezawa, Kazuo Inaba

{"title":"Distinct regulation of two flagella by calcium during chemotaxis of male gametes in the brown alga Mutimo cylindricus (Cutleriaceae, Tilopteridales)","authors":"Nana Kinoshita-Terauchi, Kogiku Shiba, Taiki Umezawa, Kazuo Inaba","doi":"10.1111/jpy.13422","DOIUrl":"10.1111/jpy.13422","url":null,"abstract":"Brown algal male gametes show chemotaxis to the sex pheromone that is released from female gametes. The chemotactic behavior of the male gametes is controlled by the changes in the beating of two flagella known as the anterior and posterior flagellum. Our previous study using Mutimo cylindricus showed that the sex pheromone induced an increment in both the deflection angle of the anterior flagellum and sustained unilateral bend of the posterior flagellum, but the mechanisms regulating these two flagellar waveforms were not fully revealed. In this study, we analyzed the changes in swimming path and flagellar waveforms with a high-speed recording system under different calcium conditions. The extracellular Ca2+ concentration at 10−3 M caused an increment in the deflection angle of the anterior flagellum only when ionomycin was absent. No sustained unilateral bend of the posterior flagellum was induced either in the absence or presence of ionomycin in extracellular Ca2+ concentrations below 10−2 M. Real-time Ca2+ imaging revealed that there is a spot near the basal part of anterior flagellum showing higher Ca2+ than in the other parts of the cell. The intensity of the spot slightly decreased when male gametes were treated with the sex pheromone. These results suggest that Ca2+-dependent changes in the anterior and posterior flagellum are regulated by distinct mechanisms and that the increase in the anterior flagellar deflection angle and sustained unilateral bend of the posterior flagellum may not be primarily induced by the Ca2+ concentration.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the chaotropic tolerance of the desert cyanobacterium Chroococcidiopsis sp. 029 (Chroococcidiopsales, Cyanobacteria) 对沙漠蓝藻 Chroococcidiopsis sp.

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2023-12-29 DOI: 10.1111/jpy.13414

Claudia Fagliarone, Beatriz Gallego Fernandez, Giorgia Di Stefano, Claudia Mosca, Daniela Billi

{"title":"Insights into the chaotropic tolerance of the desert cyanobacterium Chroococcidiopsis sp. 029 (Chroococcidiopsales, Cyanobacteria)","authors":"Claudia Fagliarone, Beatriz Gallego Fernandez, Giorgia Di Stefano, Claudia Mosca, Daniela Billi","doi":"10.1111/jpy.13414","DOIUrl":"10.1111/jpy.13414","url":null,"abstract":"The mechanism of perchlorate resistance of the desert cyanobacterium Chroococcidiopsis sp. CCMEE 029 was investigated by assessing whether the pathways associated with its desiccation tolerance might play a role against the destabilizing effects of this chaotropic agent. During 3 weeks of growth in the presence of 2.4 mM perchlorate, an upregulation of trehalose and sucrose biosynthetic pathways was detected. This suggested that in response to the water stress triggered by perchlorate salts, these two compatible solutes play a role in the stabilization of macromolecules and membranes as they do in response to dehydration. During the perchlorate exposure, the production of oxidizing species was observed by using an oxidant-sensing fluorochrome and determining the expression of the antioxidant defense genes, namely superoxide dismutases and catalases, while the presence of oxidative DNA damage was highlighted by the over-expression of genes of the base excision repair. The involvement of desiccation-tolerance mechanisms in the perchlorate resistance of this desert cyanobacterium is interesting since, so far, chaotropic-tolerant bacteria have been identified among halophiles. Hence, it is anticipated that desert microorganisms might possess an unrevealed capability of adapting to perchlorate concentrations exceeding those naturally occurring in dry environments. Furthermore, in the endeavor of supporting future human outposts on Mars, the identified mechanisms might contribute to enhance the perchlorate resistance of microorganisms relevant for biologically driven utilization of the perchlorate-rich soil of the red planet.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of reproductive sex-biased gene expression in Asparagopsis taxiformis (lineage 6) gametophytes 鉴定 Asparagopsis taxiformis（6 系）配子体中生殖性别基因的表达

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2023-12-29 DOI: 10.1111/jpy.13419

Zubaida Parveen Patwary, Min Zhao, Nicholas A. Paul, Scott F. Cummins

{"title":"Identification of reproductive sex-biased gene expression in Asparagopsis taxiformis (lineage 6) gametophytes","authors":"Zubaida Parveen Patwary, Min Zhao, Nicholas A. Paul, Scott F. Cummins","doi":"10.1111/jpy.13419","DOIUrl":"10.1111/jpy.13419","url":null,"abstract":"The sub-tropical red seaweed Asparagopsis taxiformis is of significant interest due to its ability to store halogenated compounds, including bromoform, which can mitigate methane production in ruminants. Significant scale-up of aquaculture production of this seaweed is required; however, relatively little is known about the molecular mechanisms that control fundamental physiological processes, including the regulatory factors that determine sexual dimorphism in gametophytes. In this study, we used comparative RNA-sequencing analysis between different morphological parts of mature male and female A. taxiformis (lineage 6) gametophytes that resulted in greater number of sex-biased gene expression in tips (containing the reproductive structures for both sexes), compared with the somatic main axis and rhizomes. Further comparative RNA-seq against immature tips was used to identify 62 reproductive sex-biased genes (59 male-biased, 3 female-biased). Of the reproductive male-biased genes, 46% had an unknown function, while others were predicted to be regulatory factors and enzymes involved in signaling. We found that bromoform content obtained from female samples (8.5 ± 1.0 mg·g−1 dry weight) was ~10% higher on average than that of male samples (6.5 ± 1.0 mg·g−1 dry weight), although no significant difference was observed (p > 0.05). There was also no significant difference in the marine bromoform biosynthesis locus gene expression. In summary, our comparative RNA-sequencing analysis provides a first insight into the potential molecular factors relevant to gametogenesis and sexual differentiation in A. taxiformis, with potential benefits for identification of sex-specific markers.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kelp and sea urchin settlement mediated by biotic interactions with benthic coralline algal species 海带和海胆通过与底栖珊瑚藻类的生物相互作用而沉降。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2023-12-26 DOI: 10.1111/jpy.13420

Brenton A. Twist, Florent Mazel, Stefanie Zaklan Duff, Matthew A. Lemay, Christopher M. Pearce, Patrick T. Martone

{"title":"Kelp and sea urchin settlement mediated by biotic interactions with benthic coralline algal species","authors":"Brenton A. Twist, Florent Mazel, Stefanie Zaklan Duff, Matthew A. Lemay, Christopher M. Pearce, Patrick T. Martone","doi":"10.1111/jpy.13420","DOIUrl":"10.1111/jpy.13420","url":null,"abstract":"Species interactions can influence key ecological processes that support community assembly and composition. For example, coralline algae encompass extensive diversity and may play a major role in regime shifts from kelp forests to urchin-dominated barrens through their role in inducing invertebrate larval metamorphosis and influencing kelp spore settlement. In a series of laboratory experiments, we tested the hypothesis that different coralline communities facilitate the maintenance of either ecosystem state by either promoting or inhibiting early recruitment of kelps or urchins. Coralline algae significantly increased red urchin metamorphosis compared with a control, while they had varying effects on kelp settlement. Urchin metamorphosis and density of juvenile canopy kelps did not differ significantly across coralline species abundant in both kelp forests and urchin barrens, suggesting that recruitment of urchin and canopy kelps does not depend on specific corallines. Non-calcified fleshy red algal crusts promoted the highest mean urchin metamorphosis percentage and showed some of the lowest canopy kelp settlement. In contrast, settlement of one subcanopy kelp species was reduced on crustose corallines, but elevated on articulated corallines, suggesting that articulated corallines, typically absent in urchin barrens, may need to recover before this subcanopy kelp could return. Coralline species differed in surface bacterial microbiome composition; however, urchin metamorphosis was not significantly different when microbiomes were removed with antibiotics. Our results clarify the role played by coralline algal species in kelp forest community assembly and could have important implications for kelp forest recovery.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13420","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139040151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environment-dependent metabolic investments in the mixotrophic chrysophyte Ochromonas 混养蛹虫草中依赖环境的新陈代谢投资。

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2023-12-23 DOI: 10.1111/jpy.13418

Gina S. Barbaglia, Christopher Paight, Meredith Honig, Matthew D. Johnson, Ryan Marczak, Michelle Lepori-Bui, Holly V. Moeller

{"title":"Environment-dependent metabolic investments in the mixotrophic chrysophyte Ochromonas","authors":"Gina S. Barbaglia, Christopher Paight, Meredith Honig, Matthew D. Johnson, Ryan Marczak, Michelle Lepori-Bui, Holly V. Moeller","doi":"10.1111/jpy.13418","DOIUrl":"10.1111/jpy.13418","url":null,"abstract":"Mixotrophic protists combine photosynthesis and phagotrophy to obtain energy and nutrients. Because mixotrophs can act as either primary producers or consumers, they have a complex role in marine food webs and biogeochemical cycles. Many mixotrophs are also phenotypically plastic and can adjust their metabolic investments in response to resource availability. Thus, a single species's ecological role may vary with environmental conditions. Here, we quantified how light and food availability impacted the growth rates, energy acquisition rates, and metabolic investment strategies of eight strains of the mixotrophic chrysophyte, Ochromonas. All eight Ochromonas strains photoacclimated by decreasing chlorophyll content as light intensity increased. Some strains were obligate phototrophs that required light for growth, while other strains showed stronger metabolic responses to prey availability. When prey availability was high, all eight strains exhibited accelerated growth rates and decreased their investments in both photosynthesis and phagotrophy. Photosynthesis and phagotrophy generally produced additive benefits: In low-prey environments, Ochromonas growth rates increased to maximum, light-saturated rates with increasing light but increased further with the addition of abundant bacterial prey. The additive benefits observed between photosynthesis and phagotrophy in Ochromonas suggest that the two metabolic modes provide nonsubstitutable resources, which may explain why a tradeoff between phagotrophic and phototrophic investments emerged in some but not all strains.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13418","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138885270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The requirement for external carbonic anhydrase in diatoms is influenced by the supply and demand for dissolved inorganic carbon 硅藻对外部碳酸酐酶的需求受溶解无机碳供需关系的影响

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2023-12-21 DOI: 10.1111/jpy.13416

Matthew Keys, Brian Hopkinson, Andrea Highfield, Abdul Chrachri, Colin Brownlee, Glen L. Wheeler

{"title":"The requirement for external carbonic anhydrase in diatoms is influenced by the supply and demand for dissolved inorganic carbon","authors":"Matthew Keys, Brian Hopkinson, Andrea Highfield, Abdul Chrachri, Colin Brownlee, Glen L. Wheeler","doi":"10.1111/jpy.13416","DOIUrl":"10.1111/jpy.13416","url":null,"abstract":"Photosynthesis by marine diatoms contributes significantly to the global carbon cycle. Due to the low concentration of CO2 in seawater, many diatoms use extracellular carbonic anhydrase (eCA) to enhance the supply of CO2 to the cell surface. While much research has investigated how the requirement for eCA is influenced by changes in CO2 availability, little is known about how eCA contributes to CO2 supply following changes in the demand for carbon. We therefore examined how changes in photosynthetic rate influence the requirement for eCA in three centric diatoms. Modeling of cell surface carbonate chemistry indicated that diffusive CO2 supply to the cell surface was greatly reduced in large diatoms at higher photosynthetic rates. Laboratory experiments demonstrated a trend of an increasing requirement for eCA with increasing photosynthetic rate that was most pronounced in the larger species, supporting the findings of the cellular modeling. Microelectrode measurements of cell surface pH and O2 demonstrated that individual cells exhibited an increased contribution of eCA to photosynthesis at higher irradiances. Our data demonstrate that changes in carbon demand strongly influence the requirement for eCA in diatoms. Cell size and photosynthetic rate will therefore be key determinants of the mode of dissolved inorganic carbon uptake.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13416","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138826256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kelps on demand: Closed-system protocols for culturing large bull kelp sporophytes for research and restoration 海带随需应变：为研究和恢复目的培养大型公牛海带孢子体的封闭系统规程

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2023-12-15 DOI: 10.1111/jpy.13413

Varoon P. Supratya, Patrick T. Martone

{"title":"Kelps on demand: Closed-system protocols for culturing large bull kelp sporophytes for research and restoration","authors":"Varoon P. Supratya, Patrick T. Martone","doi":"10.1111/jpy.13413","DOIUrl":"10.1111/jpy.13413","url":null,"abstract":"Culturing kelps for commercial, conservation, and scientific purposes is becoming increasingly widespread. However, kelp aquaculture methods are typically designed for ocean-based farms, and these methods may not be applicable for smaller scale cultivation efforts common in research and restoration. Growing kelps in closed, recirculating culture systems may address many of these constraints, yet closed system approaches have remained largely undescribed. Extensive declines of the bull kelp (Nereocystis luetkeana), an ecologically important canopy species in the Northeast Pacific, have received widespread attention and prompted numerous research and conservation initiatives. Here, we detail two approaches for cultivating N. luetkeana sporophytes in closed recirculating systems. Nereocystis luetkeana were reared as attached thalli in custom seaweed growth flumes and also free-floating in tumble culture tanks. Careful control of stocking density, water motion, aeration, and nutrient levels allowed for rapid growth and normal morphogenesis of laboratory-grown kelp. Culture systems reached up to 3 kg · m−3, and individual thalli attained lengths of up to 6 m before the trials were terminated. Our results demonstrate the potential of recirculating, closed culture systems to overcome limitations associated with traditional culture methods. Recirculating systems enable the precise control of culture conditions, improving biosecurity and facilitating cultivar development and other research. Kelps can be grown away from the ocean or outside their native ranges, and seasonal or annual species can be produced year-round without seasonal constraints.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13413","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138714925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antagonistic actions of Paucibacter aquatile B51 and its lasso peptide paucinodin toward cyanobacterial bloom-forming Microcystis aeruginosa PCC7806 挥发性贫杆菌 B51 及其套索肽 paucinodin 对蓝藻藻华形成微囊藻 PCC7806 的拮抗作用

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2023-12-10 DOI: 10.1111/jpy.13412

Yeji Cha, Wonjae Kim, Yerim Park, Minkyung Kim, Yongjun Son, Woojun Park

{"title":"Antagonistic actions of Paucibacter aquatile B51 and its lasso peptide paucinodin toward cyanobacterial bloom-forming Microcystis aeruginosa PCC7806","authors":"Yeji Cha, Wonjae Kim, Yerim Park, Minkyung Kim, Yongjun Son, Woojun Park","doi":"10.1111/jpy.13412","DOIUrl":"10.1111/jpy.13412","url":null,"abstract":"Superior antagonistic activity against axenic Microcystis aeruginosa PCC7806 was observed with Paucibacter sp. B51 isolated from cyanobacterial bloom samples among 43 tested freshwater bacterial species. Complete genome sequencing, analyzing average nucleotide identity and digital DNA–DNA hybridization, designated the B51 strain as Paucibacter aquatile. Electron and fluorescence microscopic image analyses revealed the presence of the B51 strain in the vicinity of M. aeruginosa cells, which might provoke direct inhibition of the photosynthetic activity of the PCC7806 cells, leading to perturbation of cellular metabolisms and consequent cell death. Our speculation was supported by the findings that growth failure of the PCC7806 cells led to low pH conditions with fewer chlorophylls and down-regulation of photosystem genes (e.g., psbD and psaB) during their 48-h co-culture condition. Interestingly, the concentrated ethyl acetate extracts obtained from B51-grown supernatant exhibited a growth-inhibitory effect on PCC7806. The physical separation of both strains by a filter system led to no inhibitory activity of the B51 cells, suggesting that contact-mediated anti-cyanobacterial compounds might also be responsible for hampering the growth of the PCC7806 cells. Bioinformatic tools identified 12 gene clusters that possibly produce secondary metabolites, including a class II lasso peptide in the B51 genome. Further chemical analysis demonstrated anti-cyanobacterial activity from fractionated samples having a rubrivinodin-like lasso peptide, named paucinodin. Taken together, both contact-mediated inhibition of photosynthesis and the lasso peptide secretion of the B51 strain are responsible for the anti-cyanobacterial activity of P. aquatile B51.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of six new cyanobacterial species from soil biocrusts on San Nicolas Island, California, in three genera previously restricted to Brazil 描述加利福尼亚州圣尼古拉斯岛土壤生物簇中的 6 个蓝藻新物种，其中有 3 个属以前仅限于在巴西发现

IF 2.9 3区生物学

Journal of Phycology Pub Date : 2023-12-09 DOI: 10.1111/jpy.13411

Brian M. Jusko, Jeffrey R. Johansen

{"title":"Description of six new cyanobacterial species from soil biocrusts on San Nicolas Island, California, in three genera previously restricted to Brazil","authors":"Brian M. Jusko, Jeffrey R. Johansen","doi":"10.1111/jpy.13411","DOIUrl":"10.1111/jpy.13411","url":null,"abstract":"As the taxonomic knowledge of cyanobacteria from terrestrial environments increases, it remains important to analyze biodiversity in areas that have been understudied to fully understand global and endemic diversity. This study was completed as part of a larger algal biodiversity study of the soil biocrusts of San Nicholas Island, California, USA. Among the taxa isolated were several new species in three genera (Atlanticothrix, Pycnacronema, and Konicacronema) which were described from, and previously restricted to, Brazil. New taxa are described herein using a polyphasic approach to cyanobacterial taxonomy that considers morphological, molecular, ecological, and biogeographical factors. Morphological data corroborated by molecular analysis including sequencing of the 16S rRNA gene, and the associated 16S–23S ITS rRNA region was used to delineate three new species of Atlanticothrix, two species of Pycnacronema, and one species of Konicacronema. The overlap of genera from San Nicolas Island and Brazil suggests that cyanobacterial genera may be widely distributed across global hemispheres, whereas the presence of distinct lineages may indicate that this is not true at the species level. Our data suggest that based upon global wind patterns, cyanobacteria in both Northern and Southern hemispheres of the Americas may have a more recent common ancestor in Northern Africa, but this common ancestry is distant enough that speciation has occurred since transatlantic dispersal.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138573258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0