Journal of Phycology最新文献

Critical taxonomic revision of Korean Dictyoteae describing three new species and honoring Haenyeo culture.

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-04-05 DOI: 10.1111/jpy.70010

Christophe Vieira, Jeong Chan Kang, Manon Daudinet, Shingo Akita, Myung Sook Kim

{"title":"Critical taxonomic revision of Korean Dictyoteae describing three new species and honoring Haenyeo culture.","authors":"Christophe Vieira, Jeong Chan Kang, Manon Daudinet, Shingo Akita, Myung Sook Kim","doi":"10.1111/jpy.70010","DOIUrl":"https://doi.org/10.1111/jpy.70010","url":null,"abstract":"This study re-evaluates the species diversity and taxonomy of the genera Canistrocarpus, Dictyota, and Rugulopteryx in Korea using an integrative approach combining molecular data and morphological observations. Phylogenetic analyses based on psbA, cox1, and rbcL gene sequences identified nine lineages including eight Dictyota and one Rugulopteryx. Morphological assessments corroborated these molecular findings. Of the seven species listed in recent national checklists, only two were confirmed, while the remaining species were misidentifications. Three novel species are described: Dictyota haenyeosa sp. nov., Dictyota sumbisoria sp. nov., and Dictyota taewakia sp. nov. Additionally, molecular evidence confirmed that the species historically identified as D. dichotoma in Korea corresponds to D. spathulata, a species described from Japan. This revision of Dictyota taxonomy highlights the importance of molecular tools in resolving long-standing misidentifications. The updated checklist for Korean Dictyota and Rugulopteryx includes D. bartayresiana, D. coriacea, D. haenyeosa sp. nov., D. pfaffii, D. spathulata, D. sumbisoria sp. nov., D. taewakia sp. nov., and R. okamurae. The genus Canistrocarpus is absent from the Korean flora. The Korean Dictyota and Rugulopteryx flora is predominantly endemic to the Eastern Asian region (Korea and Japan), with species such as D. coriacea, D. haenyeosa sp. nov., D. spathulata, D. sumbisoria sp. nov., D. taewakia sp. nov., and R. okamurae. Exceptions include the widely distributed, potentially introduced species D. pfaffii and D. bartayresiana, both originally described from the Caribbean. Rugulopteryx okamurae, while endemic to Korea and Japan, has also been introduced to the Atlantic and Mediterranean regions over the past two decades.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moderate levels of dissolved iron stimulate cellular growth and increase lipid storage in Symbiodinium sp.

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-03-30 DOI: 10.1111/jpy.70002

Walter Dellisanti, Swathi Murthy, Elena Bollati, Sara Prehn Sandberg, Michael Kühl

{"title":"Moderate levels of dissolved iron stimulate cellular growth and increase lipid storage in Symbiodinium sp.","authors":"Walter Dellisanti, Swathi Murthy, Elena Bollati, Sara Prehn Sandberg, Michael Kühl","doi":"10.1111/jpy.70002","DOIUrl":"https://doi.org/10.1111/jpy.70002","url":null,"abstract":"Dinoflagellates in the family Symbiodiniaceae are fundamental in coral reef ecosystems and facilitate essential processes such as photosynthesis, nutrient cycling, and calcium carbonate production. Iron (Fe) is an essential element for the physiological processes of Symbiodiniaceae, yet its role remains poorly understood in the context of cellular development and metabolic health. Here, we investigated the effect of iron availability-0-100 nM Fe(III)-on Symbiodinium sp. ITS2 type A1 cultures and quantified cellular content using flow cytometry and holotomography. Moderate levels of dissolved Fe (50 nM) enhanced growth rates and cellular content development in Symbiodinium sp., including lipids and proteins. We observed distinct growth patterns, pigment concentrations, and cellular morphology under increasing Fe concentrations, indicating the influence of iron availability on cellular physiology. Nondestructive, label-free holotomographic microscopy enabled single-cell in vivo imaging, revealing higher intracellular lipid accumulation (+57%) in response to 50 nM Fe(III) enrichment. Our findings contribute to a deeper understanding of the relationship between iron availability and Symbiodinium sp. growth and cellular development, with potential implications for coral health and reef resilience in the face of environmental stressors.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward understanding the mechanisms of Raphidiopsis raciborskii response to chill/light stress.

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-03-25 DOI: 10.1111/jpy.70008

Nada Tokodi, Ewa Latkowska, Barbara Klimczak, Kinga Kłodawska, Przemysław Malec, Anusuya Willis, Mikołaj Kokociński, Adam Antosiak, Dariusz Dziga

{"title":"Toward understanding the mechanisms of Raphidiopsis raciborskii response to chill/light stress.","authors":"Nada Tokodi, Ewa Latkowska, Barbara Klimczak, Kinga Kłodawska, Przemysław Malec, Anusuya Willis, Mikołaj Kokociński, Adam Antosiak, Dariusz Dziga","doi":"10.1111/jpy.70008","DOIUrl":"https://doi.org/10.1111/jpy.70008","url":null,"abstract":"Cyanobacteria, particularly Raphidiopsis raciborskii, are known for their harmful blooms, posing significant ecological and economic challenges in aquatic ecosystems. This study investigates the strain-specific adaptive mechanisms of five R. raciborskii strains (CS-505, CS-506, UAM/DH-KmRr, UAM/DH-BiRr, and UAM/DH-MRr) under chill/light stress (low temperature and high light intensity), focusing on growth, pigment composition, photosynthetic activity, gene expression, and toxin production. Results revealed that growth rates were significantly reduced under stress for all strains, with the Polish strain UAM/DH-MRr showing the highest resilience. Stress conditions caused a reduced chlorophyll a level and increased carotenoid/Chl a ratio, suggesting photoprotective adaptations. Photosynthetic quantum efficiency (Fv/Fm) declined during chill/light but was recovered post-stress, while electron transport rates (rETR) were not significantly altered. Enhanced respiration and increased photosynthetic oxygen evolution were observed, particularly in CS-506 and UAM/DH-MRr. Principal component analysis (PCA) highlighted pigment content and parameters related to photosynthetic electron transport as key factors influencing response; no variable-specific groupings were observed among strains regarding the broad range of parameters. Gene expression analyses revealed divergent transcriptional regulation of genes involved in photosynthesis and cylindrospermopsin (CYN) biosynthesis, with toxic CS-505 upregulating cyr genes under stress, while CS-506 down-regulated them. Intracellular CYN levels decreased under stress, yet cell quotas increased in CS-505, which warrants further investigation. Our results indicate the complexity of strain-specific responses to chill/light stress and highlight the importance of understanding physiological mechanisms for predicting and managing cyanobacterial blooms in diverse climates.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microcystin production is important for toxic Microcystis to survive long-term nitrogen starvation.

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-03-22 DOI: 10.1111/jpy.70009

Xiao-Ya Lian, Guo-Wei Qiu, Wen-Can Zheng, Jin-Long Shang, Hai-Feng Xu, Guo-Zheng Dai, Nan-Qin Gan, Zhong-Chun Zhang, Bao-Sheng Qiu

{"title":"Microcystin production is important for toxic Microcystis to survive long-term nitrogen starvation.","authors":"Xiao-Ya Lian, Guo-Wei Qiu, Wen-Can Zheng, Jin-Long Shang, Hai-Feng Xu, Guo-Zheng Dai, Nan-Qin Gan, Zhong-Chun Zhang, Bao-Sheng Qiu","doi":"10.1111/jpy.70009","DOIUrl":"https://doi.org/10.1111/jpy.70009","url":null,"abstract":"Toxic cyanobacterial blooms have expanded and intensified on a global scale. Although microcystins are known as the most abundant cyanotoxins released during cyanobacterial blooms, the physiological role of these toxic secondary metabolites has not been fully resolved. Here, we show that microcystin production is important for toxic Microcystis to maintain carbon metabolism under long-term nitrogen starvation and subsequent recovery. Compared to carbon metabolism in the nonmicrocystin-producing strains, toxic Microcystis could accumulate more carbon reserves under nitrogen limitation, which is important for the survival of cells under stressful conditions. Transcriptomic analysis revealed that the genes involved in microcystin synthesis were significantly up-regulated at the initial recovery phase, indicating their essential role in strengthening glycogen catabolism and fueling recovery. Flow cytometry analysis showed that compared to nontoxic strains, microcystin-producing Microcystis exhibited a higher survival and recovery rate after prolonged nitrogen starvation, which is consistent with the dominance of these species at the early stage of cyanobacterial blooms. The close genetic traits between Microcystis strains suggest that the strategies observed here might be highly conserved. Our results imply that toxic Microcystis establishes a competitive advantage over nontoxic species and provides insights into the seasonal succession of natural Microcystis populations.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic review of Tryblionella with special reference to the Apiculatae group-New characters of genus Tryblionella sensu stricto (Bacillariaceae). Tryblionella 分类学综述，特别关注 Apiculatae 群--严格意义上的 Tryblionella 属（Bacillariaceae）的新特征。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-03-18 DOI: 10.1111/jpy.70004

Rafał M Olszyński, Ewa Górecka, Rosa Trobajo, Romain Gastineau, Matt Ashworth, David G Mann

{"title":"Taxonomic review of Tryblionella with special reference to the Apiculatae group-New characters of genus Tryblionella sensu stricto (Bacillariaceae).","authors":"Rafał M Olszyński, Ewa Górecka, Rosa Trobajo, Romain Gastineau, Matt Ashworth, David G Mann","doi":"10.1111/jpy.70004","DOIUrl":"https://doi.org/10.1111/jpy.70004","url":null,"abstract":"Tryblionella (Bacillariaceae) was described by Smith in 1853, and for many years, the diagnosis of this genus was questionable. Recent molecular analysis based on the rbcL gene marker suggests that Tryblionella is a polyphyletic genus with T. apiculata, T. hungarica, and T. gaoana forming a distinct group from other Tryblionella representatives. Therefore, this study aimed to clarify the diagnosis of Tryblionella as a genus. The focus of this study was a selected group of species previously categorized within Grunow's section Apiculatae, which includes the type species T. acuminata. This classification serves as a foundation for conducting morphological and molecular comparisons with taxa of Tryblionella sensu lato, which are likely to represent distinct and highly diverse genera. Our review includes a detailed examination of frustule ultrastructure and ontogeny combined with a new molecular phylogenetic analysis derived from a three-gene concatenated dataset. The results of our research indicated that among Tryblionella, several monophyletic groups of taxa can be distinguished, including Tryblionella sensu stricto (s.s.), by three key characters: a porose valve cross-section; longitudinal valve undulation, where the peak of the undulation is located on the proximal side of the valve; and the presence of an axial sternum with thickened and relief virgae. Tryblionella s.s. taxa share a similar girdle structure: The girdle is graded, except that the first band bears a single row of poroids in the pars exterior and a crenulate margin on the side corresponding to the distal valve mantle.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of environmental DNA monitoring for seaweed reproductive phenology: A case study with giant kelp (Macrocystis pyrifera). 环境 DNA 监测在海藻生殖物候学中的应用：巨型海带（Macrocystis pyrifera）案例研究。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-03-18 DOI: 10.1111/jpy.70000

Madeline R Ward, Christopher P Burridge, Sharee McCammon, Adam Smolenski, Catriona L Hurd, Wouter Visch

{"title":"Applications of environmental DNA monitoring for seaweed reproductive phenology: A case study with giant kelp (Macrocystis pyrifera).","authors":"Madeline R Ward, Christopher P Burridge, Sharee McCammon, Adam Smolenski, Catriona L Hurd, Wouter Visch","doi":"10.1111/jpy.70000","DOIUrl":"https://doi.org/10.1111/jpy.70000","url":null,"abstract":"Monitoring the seasonal reproductive cycles of seaweeds is crucial for effective population and ecosystem management, as well as mariculture seedstock collection. Traditional methods, such as visual monitoring by SCUBA diving or snorkeling, are costly, labor-intensive, and limited in temporal and spatial coverage. This study explores substituting these methods with environmental DNA (eDNA) techniques for giant kelp (Macrocystis pyrifera, order Laminariales). This laboratory study aimed to determine the minimum detectable concentration of zoospores and sporophyte tissue needed for detecting the reproductive phenology of M. pyrifera and to assess the ability and sensitivity to discriminate between life stages. The study involved syringe-filtering seawater samples through 0.45-μm pore-size filters before quantitative polymerase chain reaction (qPCR) analysis with species-specific primers. There was a strong positive correlation between zoospore concentration and eDNA copies per μL (ρ = 0.982, p < 0.001), and a weak correlation for sporophyte wet weight (ρ = 0.367, p = 0.134). There was a significant difference between zoospore and zoospore + sporophyte treatments (p = 0.010), indicating the substantial influence of sporophyte tissue on detected eDNA quantity. Sporophyte tissue obscures the zoospore signal, especially at lower zoospore concentrations (<37 zoospores · mL-1), highlighting that eDNA analysis is suitable for monitoring reproductive peaks and broader patterns in seasonal reproduction cycles of giant kelp when zoospore concentrations are high.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KEIO knockout collection reveals metabolomic crosstalk in Chlorella spp.-Escherichia coli co-cultures.

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-03-12 DOI: 10.1111/jpy.70001

Cheah Yi Tong, Hiroya Tomita, Kentaro Miyazaki, Chan Juinn Chieh Derek, Kohsuke Honda

{"title":"KEIO knockout collection reveals metabolomic crosstalk in Chlorella spp.-Escherichia coli co-cultures.","authors":"Cheah Yi Tong, Hiroya Tomita, Kentaro Miyazaki, Chan Juinn Chieh Derek, Kohsuke Honda","doi":"10.1111/jpy.70001","DOIUrl":"https://doi.org/10.1111/jpy.70001","url":null,"abstract":"The interdependence between microalgae and bacteria has sparked scientific interest over years, primarily driven by the practical applications of microalgal-bacteria consortia in wastewater treatment and algal biofuel production. Although adequate studies have focused on the broad interactions and general behavior between the two entities, there remains a scarcity of study on the metabolic role of symbiotic bacteria in promoting microalgal growth. Here, we use the KEIO Knockout Collection, an Escherichia coli gene knockout mutant library, to systematically screen for genes involved in the interdependence of Chlorella sorokiniana and E. coli. By co-cultivating C. sorokiniana and E. coli knockout mutants in 96-well microplates (200 μL medium per well) under white light at 25°C, 31 potential algal growth-promoting and 56 growth-inhibiting genes out of 3985 genes were identified that enhanced (≥1.25-fold) and diminished (≤0.8-fold) the production of algal chlorophyll-a content, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) mapping of these growth-regulating genes suggested a metabolic symbiosis involving bacteria-derived cobalamin (cobU, cobC), biotin (bioB, bioF, bioC, bioD, fabF, fabH), riboflavin (fbp, guaB, gnd, guaA, zwf, purA), and 2,3-butanediol (fumB, adhE, mdh, frdB, pta, sdhC). The effects of these metabolites were further validated by supplementing the agents into the axenic algal cultures; Dose-dependent trends were observed for each metabolite, with a maximum four-fold increase in algal biomass productivity over the control. The specific growth rate of algae was increased by ≥1.27-fold and doubling time was shortened by ≥22.5%. The present results, obtained through genome-wide analyses of interdependence between microalgae and bacteria, reveals multiple interactions between organisms via metabolites.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the Pyropia katadae plastid genome and comparative analysis within Bangiales species.

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-03-10 DOI: 10.1111/jpy.70007

Xianghai Tang, Xinzi Yu, Xinyu Zhu, Ka Bian, Chengzhen Meng, Yunxiang Mao

{"title":"Characterization of the Pyropia katadae plastid genome and comparative analysis within Bangiales species.","authors":"Xianghai Tang, Xinzi Yu, Xinyu Zhu, Ka Bian, Chengzhen Meng, Yunxiang Mao","doi":"10.1111/jpy.70007","DOIUrl":"https://doi.org/10.1111/jpy.70007","url":null,"abstract":"Bangiales species live in intertidal regions and suffer from stresses from a variable environment, making them suitable research objects for studying how plants adapt to nature. In this study, the plastid genome of Pyropia katadae was sequenced and compared with those of 11 previously reported Bangiales species. The Py. katadae plastid genome was 193,531 bp long and contained a single-copy region (LSC) of 149,821 bp, a small single-copy region (SSC) of 34,732 bp, and two direct repeats (DRs) 4489 bp long in between. Furthermore, we compared the plastid genomes of 12 Bangiales species. Among the 12 Bangiales plastid genomes, Bangia fuscopurpurea harbored the largest plastid genome size (196,913 bp), and Py. perforata harbored the smallest (189,789 bp). Phylogenetic analyses of shared genes indicated that Py. katadae clustered with Py. yezoensis into a single clade with a high bootstrap value. An overall high degree of similarity in gene content and arrangement among the Bangiales plastid genomes was observed. The size of the plastid genomes and that of the repeats of Pyropia were positively correlated, demonstrating that the repeats were essential for changes in the plastid genome size over a short evolutionary time. The presence of DR or approximate DR regions in most Bangiales plastid genomes indicates the existence of DR regions in their last common ancestor. The different shortened lengths of identical DR regions showed that each species experienced species-specific evolutionary events, which might cause variations in the sequences and the loss of genes. The two steps of fragment reversal could generate the DRs of Bangiales species from an ancestor in common with Florideophyceae. We identified positive selection sites in eight genes that appeared to be essential for Bangiales species to adapt to diverse environments. Our results provide essential genetic data for an in-depth understanding of the evolution and phylogeny of Bangiales species.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptic diversity within the Gonyaulax spinifera species complex, its relation to the cyst-defined species Spiniferites bentorii, S. mirabilis and S. membranaceus, with the description of Gonyaulax carbonell-mooreae sp. nov. (Gonyaulacales, Dinophyceae).

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-03-10 DOI: 10.1111/jpy.70005

Shuning Huang, Kenneth Neil Mertens, Lam Nguyen-Ngoc, Hai Doan-Nhu, Bernd Krock, Zhun Li, Doc Quang Luong, Gwenael Bilien, Vera Pospelova, Hyeon Ho Shin, Sascha Plewe, Haifeng Gu

{"title":"Cryptic diversity within the Gonyaulax spinifera species complex, its relation to the cyst-defined species Spiniferites bentorii, S. mirabilis and S. membranaceus, with the description of Gonyaulax carbonell-mooreae sp. nov. (Gonyaulacales, Dinophyceae).","authors":"Shuning Huang, Kenneth Neil Mertens, Lam Nguyen-Ngoc, Hai Doan-Nhu, Bernd Krock, Zhun Li, Doc Quang Luong, Gwenael Bilien, Vera Pospelova, Hyeon Ho Shin, Sascha Plewe, Haifeng Gu","doi":"10.1111/jpy.70005","DOIUrl":"https://doi.org/10.1111/jpy.70005","url":null,"abstract":"The fossil dinoflagellates Spiniferites bentorii, S. mirabilis, and S. membranaceus are known to inhabit recent sediments and are often used to reconstruct past sea-surface conditions. However, information on their corresponding motile cells has been rare. We isolated single cysts resembling these fossil species from China and France to yield Gonyaulax spinifera-like cells. Gonyaulax strains were also established from Viet Nam and South Korea by isolating single cells. Both cysts and cells were examined by light and scanning electron microscopy, and their LSU rRNA genes were sequenced. A new Gonyaulax species, G. carbonell-mooreae, was obtained from S. bentorii-like cysts and considered the equivalent of Spiniferites bullatus, dating back to the Campanian. Gonyaulax kunsanensis was related to S. mirabilis-like cysts. A typical S. membranaceus cyst from France yielded cells resembling G. lewisiae but shared only 75% similarity in LSU rRNA gene sequence with those from South Korea. Molecular phylogeny revealed that the pronounced apical boss is systematically significant, whereas the presence of intergonal processes is insignificant. Two ASVs of 18S rRNA V4 region were respectively identified as G. kunsanensis and G. lewisiae from the Tara Oceans metabarcoding data. Gonyaulax kunsanensis has a wide distribution in the Pacific, Indian, and Atlantic Oceans, but G. lewisiae has a restricted distribution. One strain of G. kunsanensis was examined for yessotoxin content using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), but no detectable amounts of toxins were observed. Our results uncover the hidden diversity within the G. spinifera species complex and stress the significance of cyst morphology in the taxonomy of Gonyaulax.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison of calcification mechanisms in haploid and diploid cells of the coccolithophore Calcidiscus leptoporus (Murray & Blackman 1898). 卵石藻钙华虫（Calcidiscus leptoporus）（Murray 和 Blackman，1898 年）单倍体和二倍体细胞钙化机制的比较。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-03-10 DOI: 10.1111/jpy.70006

Erin M Meyer, Alison R Taylor

{"title":"A comparison of calcification mechanisms in haploid and diploid cells of the coccolithophore Calcidiscus leptoporus (Murray & Blackman 1898).","authors":"Erin M Meyer, Alison R Taylor","doi":"10.1111/jpy.70006","DOIUrl":"https://doi.org/10.1111/jpy.70006","url":null,"abstract":"Coccolithophores are prominent marine pelagic calcifiers due to their production of calcite coccoliths. Diploid coccolithophores produce heterococcoliths intracellularly, with an organic cellulose baseplate scale acting as a nucleating substrate. However, coccolith production in the haploid life phase has not been extensively studied. Most haploid coccolithophores produce distinct holococcoliths that were previously thought to be produced in an extracellular space. However, we recently observed intracellular holococcolith production in haploid Coccolithus braarudii. We therefore compared the calcification mechanisms of the 2 N and N phases of Calcidiscus leptoporus, an understudied yet ecologically important species, to better understand ultrastructure features related to calcification and explore aspects of the calcification pathway in each life-history phase. We show that both life phases of C. leptoporus produce coccoliths intracellularly and that holococcoliths are likely nucleating on body scales in a dilated vacuole space rather than within a tightly associated coccolith vesicle (CV) as in diploid heterococcolith production. Both life phases were sensitive to the SERCA Ca2+ATPase inhibitor thapsigargin, suggesting conservation in the transcellular Ca2+ transport pathways utilized for intracellular calcification, namely through calcium accumulation in the endoplasmic reticulum. However, the Si analogue germanium led to severe disruption of the CV and coccolith morphogenesis only in the diploid life phase. This work provides strong evidence to support a model of intracellular holococcolith production and clearly demonstrates that calcification in both life phases shares a common cellular pathway. Nevertheless, differences in the spatial arrangement of the CV membrane exert significant control over crystal growth that has profound consequences for coccolith morphology.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0