Journal of Phycology最新文献

{"title":"Dive surveys and eDNA detect contrasting macroalgal community composition in Nova Scotia, Canada","authors":"Cody M. Brooks,&nbsp;Claudio DiBacco,&nbsp;Meghan C. McBride,&nbsp;Kira A. Krumhansl","doi":"10.1111/jpy.70208","DOIUrl":"10.1111/jpy.70208","url":null,"abstract":"<p>Morphological identification of macroalgae can be time-consuming and species may be difficult to observe, collect, or identify, preventing detailed study on the distribution and ecology of many species. The use of eDNA in species surveillance and biodiversity assessments has gained considerable traction in studying macroalgae, as it provides an opportunity for increased sampling efficiency and a reduced reliance on taxonomic expertise for morphological identification. However, it is crucial to understand how eDNA tools may bias results of species diversity and detection and how these biases differ from traditional morphological surveys. The present study compared species composition and detection rates of dive quadrat collections with a combination of morphological identification and DNA barcoding (i.e., traditional survey methods) to two types of eDNA tools: quantitative polymerase chain reaction (qPCR) using four species-specific qPCR assays and metabarcoding using two metabarcoding markers. Metabarcoding detected nearly two times more species than traditional surveys; however, species overlap was as low as 19.2%, and some large conspicuous and ecologically important macroalgae were not detected by metabarcoding. This result suggests that metabarcoding uncovers a host of taxa largely distinct from dive surveys. Metabarcoding and qPCR proved capable of detecting target species even at very low abundances, but both showed a high incidence of false negatives. Results presented here support a combined approach leveraging the considerable strengths of eDNA in concert with species validation and abundance metrics provided by tradtional surveys.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1510-1524"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70208","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional analysis of class 3 lipases associated with triacylglycerol metabolism in Nannochloropsis oceanica (Eustigmatophyceae)","authors":"Masako Iwai,&nbsp;Koichi Hori,&nbsp;Tomokazu Kurita,&nbsp;Kumiko Okazaki,&nbsp;Shinichiro Maeda,&nbsp;Akihide Takami,&nbsp;Takashi Yamamoto,&nbsp;Atsushi Sakamoto,&nbsp;Hiroyuki Ohta,&nbsp;Mie Shimojima","doi":"10.1111/jpy.70203","DOIUrl":"10.1111/jpy.70203","url":null,"abstract":"<p>Microalgae accumulate triacylglycerols (TAGs) as storage lipids in response to nutrient limitation, yet the mechanisms governing TAG turnover remain incompletely understood. In the oleaginous microalga <i>Nannochloropsis oceanica</i>, functional studies of TAG degradation have focused on lipases containing patatin domains, whereas the roles of other lipase families remain poorly characterized. To address this shortcoming, we focused on class 3 lipases, a group distinct from patatin-domain TAG lipases, and examined their roles in TAG metabolism in <i>N. oceanica</i>. Based on genome sequencing information and domain-based screening, 23 putative class 3 lipases were identified and classified into two major orthogroups, including a lineage-specific group unique to <i>Nannochloropsis</i>. Transcriptomic data obtained under nutrient-deficient conditions were used to prioritize candidate genes for functional analysis. Targeted gene-disruption mutants were examined under phosphate-deficient conditions, which induce TAG accumulation while causing only relatively mild growth inhibition. Disruption of the <i>No3LIP6</i> or <i>No3LIP14</i> gene was consistently associated with increased TAG accumulation without severe growth defects, whereas other class 3 lipase mutants exhibited growth-linked or condition-dependent effects on TAG accumulation. In addition, disruption of selected class 3 lipases resulted in opposing changes in TAG-associated fatty-acid composition, suggesting differential contributions to TAG remodeling rather than uniform effects on TAG hydrolysis. Together, these results indicate that TAG turnover in <i>N. oceanica</i> grown under phosphate-deficient conditions is associated with lipases beyond the patatin-domain family, with a specific subset of class 3 lipases implicated in this process. This study highlights the functional relevance of non-patatin TAG lipases in microalgal TAG metabolism.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1478-1493"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148411713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative plastome analyses and genomic resource development for five Neoporphyra haitanensis and two Neopyropia yezoensis cultivars based on genome skimming data","authors":"Congcong Zhang,&nbsp;Zhicong Gao,&nbsp;Haotian Wang,&nbsp;Wei Zhou,&nbsp;Yinyin Deng,&nbsp;Rui Yang,&nbsp;Haimin Chen,&nbsp;Juanjuan Chen,&nbsp;Shanshan Zhu","doi":"10.1111/jpy.70205","DOIUrl":"10.1111/jpy.70205","url":null,"abstract":"<p><i>Neoporphyra haitanensis</i> and <i>Neopyropia yezoensis</i> are two economically important seaweeds in Asia, yet their germplasm identification is often hindered by high phenotypic plasticity and limited molecular resources. To address this, we utilized a genome skimming strategy on five <i>No. haitanensis</i> and two <i>Ny. yezoensis</i> cultivars to retrieve their plastome information (including whole plastome sequences, plastome-divergent hotspots, and plastome-derived SSRs) and to identify genome-wide polymorphic nuclear SSRs. The plastomes of <i>No. haitanensis</i> (201,110–201,310 bp) were markedly larger than those of <i>Ny. yezoensis</i> (191,974 bp), a difference largely driven by expansions in intergenic regions. Gene annotation identified 253 genes in <i>No. haitanensis</i> and 256 in <i>Ny. yezoensis</i>, with the latter uniquely containing two additional hypothetical ORFs (ORF33 and ORF35) and an extra rRNA gene (<i>rrf</i>B). Further comparative analysis revealed seven hypervariable regions (π &gt; 0.1), five of which—<i>apc</i>E<i>-tat</i>C, <i>psb</i>W<i>-trn</i>R, <i>psb</i>X<i>-acc</i>D, <i>trn</i>W<i>-rpl</i>11, and <i>ycf</i>32<i>-rpl</i>32—are intergenic spacers with strong potential as species-discriminatory barcodes. We also characterized 26–30 chloroplast-derived SSRs and identified notable interspecific variations in their repeat-type profiles. Importantly, we developed 230 and 505 high-quality polymorphic nuclear SSRs for <i>No. haitanensis</i> and <i>Ny. yezoensis</i>, respectively, predominantly trinucleotide repeats, offering a robust toolset for fine-scale genetic analyses. Phylogenetic reconstruction using 201 shared plastid protein-coding genes clearly separated the two species into distinct clades, supporting their independent evolutionary trajectories, and further revealed intraspecific genetic differentiation, particularly with the cultivar ZD-1 forming a distinct branch within <i>No. haitanensis</i>. This study provides comprehensive genomic resources—including complete plastomes, hypervariable regions, and polymorphic SSR markers—that will facilitate germplasm identification, phylogenetic studies, and molecular breeding in <i>Porphyra</i> sensu lato.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1525-1535"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overlooked components of lipid metabolism: Odd-chain polyunsaturated fatty acids in photosynthetic microorganisms","authors":"Linda Nedbalová,&nbsp;Milada Vítová,&nbsp;Tomáš Řezanka","doi":"10.1111/jpy.70211","DOIUrl":"10.1111/jpy.70211","url":null,"abstract":"<p>This mini-review examines odd-carbon polyunsaturated fatty acids (odd-PUFAs), a rare but biologically significant group of fatty acids in photosynthetic microorganisms. Odd-PUFAs are straight-chain fatty acids containing an odd number of carbon atoms and two or more methylene-interrupted double bonds. They typically account for less than 1% of total fatty acids in microalgae and cyanobacteria. Current evidence indicates that their biosynthesis primarily originates from propionyl-CoA-initiated fatty acid synthesis, followed by conventional elongation and desaturation reactions. The review focuses on three major themes. First, it addresses analytical challenges in odd-PUFA identification, emphasizing the limitations of gas chromatography with a flame ionization detector (GC-FID) retention-time assignments and the need for complementary tandem mass spectrometry (MS/MS)-based approaches and derivatization techniques for reliable structural characterization. Second, it summarizes the current knowledge of odd-PUFA biosynthesis, including the metabolic origins of propionyl-CoA and its incorporation into fatty acid synthase pathways. Third, it surveys the occurrence of odd-PUFAs across photosynthetic microorganisms, ranging from trace constituents in cyanobacteria and chlorophytes to the characteristic profiles of <i>Euglena gracilis</i> and the very-long-chain odd-PUFAs in dinoflagellates. The primary emphasis is on photosynthetic microorganisms, although thraustochytrids are included as a comparative group because they represent the best characterized microbial system for odd-chain PUFA biosynthesis. Higher trophic organisms are discussed only briefly to illustrate the transfer of odd-PUFA algal through aquatic food webs. Advances in lipidomics and the growing interest in odd-chain fatty acids highlight the need for more research, including analytical standardization, broader taxonomic surveys, and metabolic engineering approaches for biotechnological production.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1428-1439"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148592257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved primary production and gross respiration measurements using the H218O technique with membrane inlet mass spectrometry and introduction of a small volume–uniform irradiance incubation chamber","authors":"Bruna F. Sobrinho,&nbsp;Nayani K. Vidyarathna,&nbsp;Todd M. Kana,&nbsp;Patricia M. Glibert","doi":"10.1111/jpy.70234","DOIUrl":"10.1111/jpy.70234","url":null,"abstract":"<p>An improvement in the H₂¹⁸O technique for phytoplankton gross primary productivity (GPP), net primary productivity (NPP), and gross respiration (GR) is described that reduces cost, labor, and time over the traditional isotope ratio mass spectrometer (IRMS) method. Membrane inlet mass spectrometry (MIMS) was optimized to measure small (3 mL) samples with low isotope enrichment (1% ¹⁸O) and at high analytical precision (~1 per mil). A photosynthetron was used to measure up to 10-point light curves of GPP, NPP, and GR using a total of 50 mL of sample water with an isotope cost of &lt;$15 per experiment. A new incubation device is described, a Coronatron, that allows up to 12 samples with nutrient manipulation under uniform irradiance. Example light curves from highly contrasting natural water samples are presented that show elevated GPP relative to NPP and substantial light-stimulated GR. To highlight the improved method, examples nutrient manipulation curves are also presented showing GPP, NPP, and GR as a function of changes in ambient nitrate concentrations and different temperatures under constant light.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1440-1450"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70234","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do we need to sequence all algal type specimens?","authors":"Olivier De Clerck,&nbsp;Frederik Leliaert,&nbsp;Silke Bouckenooghe,&nbsp;Heroen Verbruggen","doi":"10.1111/jpy.70222","DOIUrl":"10.1111/jpy.70222","url":null,"abstract":"<p>Recent studies sequencing algal type specimens, particularly in the green seaweed genus <i>Ulva</i>, have resolved longstanding nomenclatural uncertainties but have also triggered extensive name changes through the strict application of the principle of priority. Because the identity of a taxon name, for example, the name of a species, is ultimately determined by its nomenclatural type, sequencing type specimens can provide a powerful means to connect historical names with contemporary molecular species concepts. However, we argue that comprehensive type sequencing is unlikely to provide a practical or lasting route to nomenclatural stability across algae. The large backlog of historical names, the continuing description of new taxa, changing species concepts, and substantial technical and logistical challenges associated with sequencing historical material suggest that a complete sequence-based nomenclatural framework will remain difficult to achieve. In addition, repeated nomenclatural changes may reduce the accessibility and usability of accumulated biological knowledge for both specialists and non-specialists. Here, we invite the phycological community to reflect on the balance of taxonomic accuracy, scientific progress, and nomenclatural stability. We discuss the benefits and drawbacks of alternative mechanisms already available under the <i>International Code of Nomenclature</i>, including epitypification and conservation of names, and highlight the concept of protection, currently available for fungi, as potential approaches to promote nomenclatural stability in algae.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1423-1427"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148723859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluctuating asymmetry based on fractal geometry does not track environmental stress in an intertidal habitat-forming fucoid","authors":"Antonio Flores-Moya,&nbsp;Ignacio J. Melero-Jiménez,&nbsp;María J. García-Sánchez,&nbsp;Elena Bañares-España","doi":"10.1111/jpy.70221","DOIUrl":"10.1111/jpy.70221","url":null,"abstract":"<p>The availability of easy-to-measure and inexpensive biomarkers is a challenge for monitoring environmental stress in organisms. Although it has been proposed that non-directional deviations from a symmetrical pattern in morphological traits (called fluctuating asymmetry. FA) may be directly related to environmental stress, this postulate has been questioned. Self-similarity symmetry (fractal) is one possible approach to addressing FA analysis, but there are very few studies with this approach, and the results have been inconclusive. Here, we evaluate whether fractal descriptors of thallus morphology in the habitat-forming fucoid <i>Fucus limitaneus</i> reflect environmental stress across multiple contexts, including seasonal variation, vertical gradients in the intertidal zone, and the possible long-term impact of an invasive species. Our results show that fractal metrics did not vary consistently across these contrasting environmental conditions, suggesting that in this system, FA based on fractal geometry does not reliably track environmental stress. This is possibly because stress is expressed through alternative ecological and biomechanical processes rather than through modifications of thallus self-similarity.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1597-1605"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70221","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148723290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pan-genome perspective uncovers the core genetic basis and evolutionary adaptation of lipid synthesis and vesicular transport in Nannochloropsis","authors":"Pengjuan Zhang,&nbsp;Lijun Miao,&nbsp;Hua Wang,&nbsp;Jing Wang,&nbsp;Ge Zhang","doi":"10.1111/jpy.70218","DOIUrl":"10.1111/jpy.70218","url":null,"abstract":"<p><i>Nannochloropsis</i> microalgae are widely recognized as sustainable cell factories for producing nutritional oils and biofuels due to their high-lipid content. However, a comprehensive understanding of the genetic basis of their oleaginous traits across diverse species has been limited. Here, we constructed a pan-genome of 17 <i>Nannochloropsis</i> species comprising 14,851 gene families. Our analysis defined a distinct genetic architecture for lipid metabolism: Gene families associated with vesicular transport formed a conserved core functional module, whereas the genetic collection for lipid metabolism showed greater plasticity and was primarily classified as part of the soft-core genome. This finding establishes a genetic blueprint for the coevolution between a stable cellular “logistics network” and an adaptable “biosynthetic factory.” Evolutionary analysis further indicated that the DGAT and fatty acid desaturase families have species-specific expansions in <i>Nannochloropsis</i>, suggesting a potential role in enhancing lipid accumulation. By integrating 231 transcriptome datasets, we identified key genes (ACP2 and DGAT2) that were highly upregulated under nitrogen deprivation and pinpointed a set of core genes with high expression levels involved in vesicular transport. This “Infrastructure–Toolkit” model provides both genetic targets for strain improvement and a broader framework for understanding lipid accumulation in oleaginous microorganisms.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1656-1670"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Euglena sp. and its associated prokaryotic community degrade light crude oil","authors":"Michelle Kulbatzki,&nbsp;Hilal Kaya,&nbsp;Sabina Marks,&nbsp;Lisa Voskuhl","doi":"10.1111/jpy.70227","DOIUrl":"10.1111/jpy.70227","url":null,"abstract":"<p>Oil enters the environment through natural and anthropogenic sources and poses a serious threat to water and soil. We hypothesized that algae and their associated prokaryotic communities from natural oil seeps are robust systems capable of surviving and degrading anthropogenic oil spills. We isolated <i>Euglena</i> sp. together with its closely associated prokaryotes from a natural oil seep in Germany and tested its oil degradation potential in laboratory cultivation experiments. Oil mineralization was monitored using reverse stable isotope labeling (RSIL), and the associated microbiome was characterized by 16S rRNA gene amplicon sequencing over a 76-day incubation. Dark incubations of <i>Euglena</i> sp. showed significantly higher oil degradation than day/night light cycle incubations. The RSIL data and microbial community dynamics indicated that oil degradation was primarily driven by <i>Euglena</i> sp. itself rather than by the associated prokaryotes. We conclude that the <i>Euglena</i> sp. culture SLN082 is a promising model for studying algal oil degradation and has potential for bioremediation applications. Comprehensive information on <i>Euglena</i> sp. isolation and experimental protocols, such as cultivation, RSIL measurements, DNA extraction and 16S rRNA gene amplicon sequencing, and subsequent bioinformatic analyses, is available in the supplementary text.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1417-1422"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70227","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148906368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitogenome structure and phylogenomics of the order Thoreales (Nemaliophycidae, Rhodophyta)","authors":"Monica O. Paiano,&nbsp;Morgan L. Vis,&nbsp;Nadia M. Lemes da Silva,&nbsp;John A. West,&nbsp;Shiva K. Rai,&nbsp;Javier Carmona-Jiménez,&nbsp;Orlando Necchi Jr","doi":"10.1111/jpy.70207","DOIUrl":"10.1111/jpy.70207","url":null,"abstract":"<p>Organelle genomes offer a powerful tool for red algal evolutionary studies. Although the number of Rhodophyta genomes has increased substantially over the past 2 decades, only two mitochondrial genomes have been reported for the red algal order Thoreales. In this study, we generated 10 new complete mitochondrial genomes of Thoreales, representing the order's two genera and eight species, which, combined with additional mitogenomes of related orders of the subclass Nemaliophycidae, resulted in a comprehensive dataset of 20 mitogenomes. Thoreales mitogenomes exhibited little variation, ranging in length from 25,022 bp to 26,369 bp, with GC content from 25.6% to 28.3%, gene numbers from 46 to 54, and protein-coding genes from 21 to 25. Four genes were missing in one or more species (<i>atp</i>4, <i>rpl</i>20, <i>sdh</i>2, and <i>sdh</i>4), and no introns were detected. Synteny among members of Thoreales was highly conserved and similar to other orders of Nemaliophycidae. Phylogenomic analyses strongly supported the monophyly of Thoreales within Nemaliophycidae. Within the order, <i>Nemalionopsis</i> and <i>Thorea</i> as well as the relationship among the species received full support. <i>Nemalionopsis shawii</i> and <i>N. parkeri</i> formed an early-diverging clade that was sister to <i>Thorea</i>, which had species that resolved into two distinct lineages lacking evident geographic or morphological differentiation. By expanding the available dataset from two to 10 mitogenomes, this study provides a broad organellar perspective on Thoreales evolution, contributing to new insights into the systematics and diversification of freshwater red algae and, potentially, for clarifying inter-ordinal relationships within Nemaliophycidae.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 5","pages":"1451-1460"},"PeriodicalIF":4.3,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70207","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}