牛海带(褐海带科)种质的一种简单有效的低温保存方法。

IF 2.8 3区 生物学 Q1 MARINE & FRESHWATER BIOLOGY
Liam J M Coleman, Silven Read, Amnit K Sokhey, Sherryl Bisgrove
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引用次数: 0

摘要

海带是一种巨大的棕色海藻,可以形成三维的水下森林,为各种海洋生物提供食物和栖息地。它们还为人类提供了丰富的生态系统服务,并可能通过蓝碳帮助应对气候变化。然而,目前世界上许多地方的海带数量正在减少,很可能是由于海洋温度上升,需要迅速采取保护行动来保护海带的生物多样性。一个需要进一步发展的海带保护策略是生物银行,即生物材料的储存。特别是,低温保存方案的发展将使在稳定条件下更容易地储存大量海带种质。在本文中,我们比较了不同的冷冻保护剂(化学物质减轻冷冻对活组织的破坏作用)在牛海带配子体组织冷冻保存中的效果。我们观察到,当在10%乙二醇+ 9%山梨醇的溶液中低温保存时,Nereocystis两性配子体在低温条件下移除6周后表现出良好的存活率。尽管海带低温保存技术仍需进一步研究,但我们相信这些方法在海带生物库的改进和扩展方面具有很大的潜力,我们将鼓励更多海带物种的低温保存技术的发展,并在现有海带生物库的基础上更广泛地采用低温保存技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A simple and effective protocol for cryopreservation of germplasm of the bull kelp, Nereocystis luetkeana (Phaeophyceae).

Kelps are large brown seaweeds that can form three-dimensional underwater forests that provide food and habitat for a wide diversity of marine organisms. They also provide a wealth of ecosystem services to humans and may be able to help combat climate change through blue carbon. However, kelps are currently in decline in many parts of the world, most likely due to rising ocean temperatures, and conservation action is needed quickly to preserve kelp biodiversity. One kelp conservation strategy that needs further development is biobanking, the storage of biological material. In particular, the development of cryopreservation protocols would permit easier storage of large quantities of kelp germplasm under stable conditions. In this paper, we compare the effectiveness of different cryoprotective agents-chemicals that mitigate the damaging effects of freezing on living tissue-for use in cryopreservation of gametophyte tissue of the bull kelp, Nereocystis luetkeana. We observed that when cryopreserved in a solution of 10% ethylene glycol + 9% sorbitol, Nereocystis gametophytes of both sexes showed excellent survivorship 6 weeks after removal from cryogenic conditions. Although kelp cryopreservation protocols still need to be further researched, we believe that these methods have great potential to improve and expand kelp biobanking, and we would encourage the development of protocols for more kelp species as well as more widespread adoption of cryopreservation by existing kelp biobanking efforts.

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来源期刊
Journal of Phycology
Journal of Phycology 生物-海洋与淡水生物学
CiteScore
6.50
自引率
3.40%
发文量
69
审稿时长
2 months
期刊介绍: The Journal of Phycology was founded in 1965 by the Phycological Society of America. All aspects of basic and applied research on algae are included to provide a common medium for the ecologist, physiologist, cell biologist, molecular biologist, morphologist, oceanographer, taxonomist, geneticist, and biochemist. The Journal also welcomes research that emphasizes algal interactions with other organisms and the roles of algae as components of natural ecosystems. All aspects of basic and applied research on algae are included to provide a common medium for the ecologist, physiologist, cell biologist, molecular biologist, morphologist, oceanographer, acquaculturist, systematist, geneticist, and biochemist. The Journal also welcomes research that emphasizes algal interactions with other organisms and the roles of algae as components of natural ecosystems.
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