Journal of Phycology最新文献

{"title":"Novel Hydrurus species (Chrysophyceae) and their adaptations to high-altitude European and Arctic snowfields","authors":"Lenka Procházková,&nbsp;Robert A. Andersen,&nbsp;Thomas Leya,&nbsp;Tomáš Řezanka,&nbsp;Martin Lukeš,&nbsp;Linda Nedbalová,&nbsp;Daniel Remias","doi":"10.1111/jpy.70162","DOIUrl":"10.1111/jpy.70162","url":null,"abstract":"<p>Colored snow caused by green algae (Chlorophyceae) is well known, but melting snowpacks can also harbor golden-brown blooms consisting of Chrysophyceae. We collected 14 samples of cryoflora in the Austrian and Swiss Alps, the High Tatras in Slovakia, and in Arctic Svalbard. Eight laboratory unicellular flagellated strains were established from eight sites and phylogenetic analyses (18S rRNA and <i>rbc</i>L gene sequences) revealed new taxa belonging to the order Hydrurales (Chrysophyceae). Some formed tetrahedral swarmers; others were capsoid or amoeboid forms. Characteristics of vegetative cells and molecular markers, including the ITS2 rRNA region, supported the description of eight species: <i>Hydrurus novisii</i> sp. nov., <i>H. klavenessii</i> sp. nov., <i>H. tatrae</i> sp. nov., <i>H. pulcher</i> sp. nov., <i>H. pascheri</i> sp. nov., <i>H. svalbardensis</i> sp. nov., <i>H. nivalis</i> sp. nov. and <i>H. nemcovae</i> sp. nov. Pulse-amplitude-modulate (PAM) fluorometry indicated that the photosystem II of Arctic populations was adapted to high light conditions. Abundant polyunsaturated fatty acids supported cell survival at temperatures around 0°C, and the composition of these acids differed among species. The cells contained compatible solutes that could act as antifreeze agents. The main carotenoid fucoxanthin caused the overall golden-brown pigmentation. The closest relatives of the new species were reported from snow and cold mountain streams and lakes, indicating that these Hydruralian microalgae prefer low temperatures and elevated irradiation. The large number of new species discovered during this limited sampling campaign suggests the underestimated diversity of phototrophic microbes in melting snow. Consequently, the genus <i>Hydrurus</i> shows a similar high relevance for snow algae blooms as <i>Chloromonas</i> does within the green algae.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"818-845"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal succession and niche differentiation in Skeletonema species driven by temperature and salinity in inner Tokyo Bay","authors":"Toshiya Katano,&nbsp;Tomohiro Suematsu,&nbsp;Ayane Tanaka,&nbsp;Saori Yasui-Tamura,&nbsp;Fuminori Hashihama","doi":"10.1111/jpy.70168","DOIUrl":"10.1111/jpy.70168","url":null,"abstract":"<p>The genus <i>Skeletonema</i> is a dominant diatom in coastal waters worldwide, frequently causing blooms, and it includes several cryptic species. To elucidate the occurrence patterns and niche differentiation among <i>Skeletonema</i> species in Tokyo Bay, Japan, sampling was conducted between June 2021 and February 2023. Species composition and abundance were determined using species-specific quantitative polymerase chain reaction (qPCR) and were analyzed using an environmental framework in temperature, salinity, and nutrient concentrations. Niche characteristics were determined using outlying mean index (OMI) analysis, focusing on five frequently detected species: <i>S. ardens</i>, <i>S. marinoi–dohrnii</i> complex, <i>S. menzelii</i>, <i>S. potamos</i>, and <i>S. japonicum</i>. Seasonal succession was evident, wherein <i>S. japonicum</i> predominated in winter, followed by the <i>S. marinoi–dohrnii</i> complex in spring and <i>S. menzelii</i>, <i>S. ardens</i>, and <i>S. potamos</i> in summer. Principal component analysis indicated that <i>S. japonicum</i> was associated with low temperature and high salinity, whereas other frequently detected species were positively correlated with temperature. The OMI analysis revealed that niche breadth differed among species, with <i>S. potamos</i>, <i>S. marinoi–dohrnii</i> complex, and <i>S. menzelii</i> showing a wide niche breadth and <i>S. ardens</i> and <i>S. japonicum</i> being specialists. Marginality was highest for <i>S. potamos</i>, indicating an association with low salinity, but it was lowest for <i>S. japonicum</i> due to a high frequency of high-salinity conditions. This study demonstrated that (1) seasonal succession in the bay is primarily structured by associations with temperature and salinity but (2) niche partitioning is not always clear. When the environment was highly disturbed in the summer rainy season, more than two <i>Skeletonema</i> species frequently co-occurred, suggesting potential competition for nutrients.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"931-942"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70168","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and secondary metabolites of the marine cyanobacterium Capilliphycus salinus ALCB114379","authors":"Gabriel Schimmelpfeng Passos,&nbsp;Rafael Barty Dextro,&nbsp;Mauricio Junior Machado,&nbsp;Anderson Miguel Teixeira Feitosa,&nbsp;Núbia Pereira Silva,&nbsp;Renata Beatriz Cruz,&nbsp;Ana Paula Dini Andreote,&nbsp;Ernani Pinto,&nbsp;Marli Fatima Fiore","doi":"10.1111/jpy.70174","DOIUrl":"10.1111/jpy.70174","url":null,"abstract":"<p>Assembling high-quality genomes from underexplored environments can be helpful for understanding microbial diversity and identifying novel species. The Cyanobacterium type strain <i>Capilliphycus salinus</i> ALCB114379 is a representative of Oscillatoriales order isolated from a supralittoral zone of the south Atlantic Ocean in Brazil, an ecotone characterized by significant environmental fluctuations due to its transitional nature between the tidal and terrestrial environments. Here, we present its genome assembled into a single contig and circularized, with a total size of 7.7 Mb and 99.67% completeness. The genome has 6204 protein-coding genes, including several involved in the biosynthesis of secondary metabolites. Despite being a homocytous genus, <i>C. salinus</i> ALCB114379 possesses a gene cluster for the biosynthesis of molybdenum-type nitrogenase, indicating a potential for N₂ fixation. Additionally, the genome contains other gene clusters related to the biosynthesis of biotechnologically relevant compounds, such as microcyclamide (<i>mca</i>), a cytotoxic cyanopeptide, and mycosporine-like amino acids (MAAs), with photoprotective and antioxidant functions. High-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) analysis showed the detectable production of three MAAs, with shinorine being the most abundantly produced compared with porphyra-334 and palythine. This work provides insights into the Cyanobacteria phylogenomics and metabolism, highlighting the potential of <i>C. salinus</i> ALCB114379 as a source of specific metabolites for biotechnological applications.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"904-916"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoplankton growth and potential cyanotoxin production differ in response to nitrogen and phosphorus amendments in late summer communities from Kabetogama Lake (Minnesota, United States)","authors":"James H. Larson,&nbsp;Ryan P. Maki,&nbsp;Sean W. Bailey,&nbsp;Victoria G. Christensen,&nbsp;Keith A. Loftin,&nbsp;Erin A. Stelzer,&nbsp;James C. Smith,&nbsp;Seth A. McWhorter","doi":"10.1111/jpy.70166","DOIUrl":"10.1111/jpy.70166","url":null,"abstract":"<p>Cyanotoxins such as microcystin (MC), cylindrospermopsin, and saxitoxin are secondary metabolites that are rich in nitrogen (N). Most cyanobacteria grow best on reduced inorganic N (ammonium, NH₄), but when NH₄ is absent, cyanobacteria can activate physiological pathways to process other N forms (e.g., nitrate; NO₃). Studies on some cyanobacterial cultures have indicated that expression of N stress response genes is associated with MC gene expression. If accurate, NH₄ additions could reduce N stress and therefore cyanotoxin production. Lakes experiencing changes in N form and supply could thus experience changes in cyanobacterial toxicity independent of shifts in abundance. We performed nutrient amendments on phytoplankton communities from Kabetogama Lake (Minnesota, United States) over 2 years (four experiments total), to assess the role of N and phosphorus amendments on growth and toxicity of phytoplankton. Natural communities were collected during cyanobacterial blooms and exposed to short laboratory experiments with amendments of NH₄, NO₃, orthophosphate, or a combination of all three. In three of four experiments, biomass responses were consistent with N-limitation based on chlorophyll <i>a</i> or biovolume estimates. In experiments with evidence for N-limited growth, MC gene expression was lower in NH₄ treatments than in the control in 2021 but higher than in the control in 2022. The proportion of heterocytes (specialized cells for N fixation) was positively correlated with MC gene expression. These experiments reinforce the strong connection between N physiology and MC gene expression, but variation in taxonomic composition within genera or even species remains a possible cause of inconsistency in whole-community responses.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"883-903"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70166","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are integrative systematic tools efficient toward unraveling species diversity with the genus Jania (Corallinaceae, Rhodophyta)?","authors":"Clio Maridakis,&nbsp;Viviana Peña Freire,&nbsp;Gilberto Marani,&nbsp;Maria Tarin Sancho,&nbsp;Line Le Gall,&nbsp;Florence Rousseau","doi":"10.1111/jpy.70167","DOIUrl":"10.1111/jpy.70167","url":null,"abstract":"<p>The articulated genus <i>Jania</i> currently comprises 54 accepted species, making it the fourth most speciose genus among corallines, following <i>Lithophyllum</i>, <i>Amphiroa</i>, and <i>Lithothamnion</i>. Unlike these other genera, <i>Jania</i> is relatively easy to identify at a generic rank. However, morpho-anatomical characters are insufficiently discriminant for species identification, making DNA sequences essential for reliable species delimitation. We evaluated species diversity within <i>Jania</i> using the most comprehensive sampling to date, spanning a broad geographic range with a focus on the Mediterranean and Caribbean regions. Our data set comprised 186 specimens from the National Herbarium of the Muséum national d'Histoire naturelle (PC), including four type specimens with the basionyms <i>Jania micrarthrodia</i>, <i>Corallina polydactyla</i>, <i>Corallina mauritiana</i>, and <i>Jania digitata</i>. We also incorporated publicly available sequences from GenBank and Barcode of Life Data System (BOLD) to delimit species and infer their phylogenetic relationships using the <i>psb</i>A and COI genes. Through an integrative taxonomic approach, combining morpho-anatomical traits, molecular systematics, and biogeography, we delineated 39 putative species—28% fewer than the currently accepted number. Most of the putative species had a restricted distribution, whereas five were widely distributed. We determined seven species from the European Atlantic and Mediterranean seas and six from the Caribbean. Furthermore, we demonstrated that intergenicular morphometry is an unreliable trait for species identification, highlighting the morphological plasticity of <i>Jania</i>. Many putative species remained unidentified (67%), while some of those putative species included specimens with different identifications. Additional sequences of type specimens would be crucial for further resolving taxonomy and bridging the gap between type-bearing name and putative species delineated based on molecular data.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"781-800"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth and development of Griffithsia heteroclada under varying light intensity and cell type conditions","authors":"Jie Lyu,&nbsp;Peng Zhao,&nbsp;Jie Liu,&nbsp;Jihae Park,&nbsp;Hojun Lee,&nbsp;Yaohui Wang,&nbsp;Taejun Han","doi":"10.1111/jpy.70183","DOIUrl":"10.1111/jpy.70183","url":null,"abstract":"<p>To investigate the effects of internal and external factors on the growth and development of <i>Griffithsia heteroclada</i>, the optimal culture medium and conditions were first established. Subsequently, individual apical cell (Ca), rhizoid cell (Cr), shoot cell (Cs), and nodal cell (Cn) were cultured under varying light intensities. The findings indicated that the most suitable culture media for <i>G. heteroclada</i> are Provasoli-enriched seawater and Tropic Marin, with optimal culture conditions comprising an irradiance of 60 μmol photons · m⁻² · s⁻¹, a salinity of 25, a temperature of 25°C, and a pH of 8. Any single cell could regenerate into a new plant, but regeneration varied by cell type, with the promoting effect being most pronounced in the order of Cn = Cs &gt; Cr ≥ Ca. High light intensity inhibited energy transfer from accessory pigments to reaction centers in Ca and Cr, thereby diminishing their light capture capacity and photosynthetic efficiency. Fresh weight is deemed an appropriate biomass indicator for <i>G. heteroclada</i>, and the cell enlargement rate is considered a suitable growth indicator. Developmental indicators include cell number, division rate, branch number, and branch initiation time, while cell length, branching rate, elongation rate, and area are not suitable.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"917-930"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148175818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoscale elemental mapping reveals the effect of light and temperature on Gephyrocapsa huxleyi coccoliths","authors":"Cory Flavin,&nbsp;Thomas Marchitto,&nbsp;Ajith Pattammattel,&nbsp;Yong S. Chu,&nbsp;Anushree Chatterjee,&nbsp;Michael F. Toney","doi":"10.1111/jpy.70178","DOIUrl":"10.1111/jpy.70178","url":null,"abstract":"<p>Coccolithophores produce vast quantities of stable calcium carbonate coccoliths, occupying the distinctive position as one of the most productive calcifying organisms on Earth. <i>Gephyrocapsa huxleyi</i>, the most abundant species of coccolithophores across modern oceans, plays an important role in the biological carbon pump and may serve as inspiration for nature-based carbon capture and sequestration technologies. Despite intensive study into <i>G. huxleyi</i>, the biological mechanisms of coccolith synthesis remain unclear. Here, we employ synchrotron X-ray fluorescence to quantitatively map elemental incorporation (calcium, Ca; strontium, Sr; sulfur, S; iron, Fe; and silicon, Si) into <i>G. huxleyi</i> coccoliths at 40-nanometer resolution. Trace elements play a role in determining the thermodynamic stability of coccolith carbonate sequestration and may provide insight into unknown mechanisms of coccolithogenesis. Although the coccolith Sr/Ca ratio has been widely applied as a paleoceanographic proxy, understanding of the Sr/Ca phenomena is limited. Our results confirm that Sr/Ca is temperature dependent but challenge the hypothesis that growth rate is a primary determinant. We find that increased light irradiance led to an ~30% decrease in calcium incorporated into the coccoliths. Surprisingly, X-ray fluorescence spectra and mapping revealed evidence of sulfur, at approximately three times the concentration of Sr, embedded in and around the calcite matrix, suggesting that <i>G. huxleyi</i> coccoliths may play an expanded role in the global sulfur cycle. Based on our elemental maps, we postulate that sulfur is active in the intracellular coccolith vesicle and plays a direct role in formation of <i>G. huxleyi</i> coccoliths.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"959-973"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148101613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conspecific alarm cues induce chemical but not morphological defenses in Pseudo-nitzschia","authors":"Shuwen Zhang,&nbsp;Tingting Zheng,&nbsp;Muyao Zhou,&nbsp;Xin Guo,&nbsp;Stephen S. Bates,&nbsp;Guilin Liu,&nbsp;Huijun Zeng,&nbsp;Jiaqi Lei,&nbsp;Yang Li","doi":"10.1111/jpy.70179","DOIUrl":"10.1111/jpy.70179","url":null,"abstract":"<p>This study investigates the chemical and morphological responses of two domoic acid (DA)-producing diatoms, <i>Pseudo-nitzschia multiseries</i> and <i>P. fukuyoi</i>, to conspecific chemical cues released from mechanically damaged cells. Cell homogenate filtrate (CHF) was added to batch cultures via single or repeated doses to simulate acute and sustained cell disruption associated with grazing. Results showed that CHF exposure did not affect growth rate, cell abundance, and morphological traits, including chain density, average chain length, and frustule silicification, in either species. In contrast, cellular DA content increased significantly in response to CHF addition, reaching up to 2.16-fold higher in <i>P. multiseries</i> and 1.44-fold in <i>P. fukuyoi</i>, particularly during the decline phase. The magnitude of these toxicogenic responses depended on species identity, growth stage, and cue delivery regime, with repeated or persistent cue exposure eliciting stronger responses. Algal physiological parameters and environmental conditions (pH, nutrients, bacterial abundance) remained largely unchanged, indicating that toxin induction was primarily driven by direct sensing of algal-derived infochemicals rather than indirect environmental effects. Notably, enhanced DA production occurred without detectable reductions in growth or cell abundance. These findings demonstrate that <i>Pseudo-nitzschia</i> can deploy efficient, chemically mediated defenses in response to conspecific alarm cues, with important implications for toxin regulation, grazer-prey interactions, and the ecological dynamics of harmful algal blooms.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"1016-1030"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epitypification of Merismopedia tranquilla (Cyanobacteria) prompts phylogenetic reassessment of the genus and the description of two morphologically similar genera","authors":"Jan Pokorný,&nbsp;Jan Kaštovský,&nbsp;Lenka Marešová Štenclová","doi":"10.1111/jpy.70169","DOIUrl":"10.1111/jpy.70169","url":null,"abstract":"<p>The genus <i>Merismopedia</i> is morphologically characterized by its distinctive colony arrangement yet exhibits considerable variability among species. Recent studies suggested that <i>Merismopedia</i> is polyphyletic, and its most likely true lineage was provisionally placed within the Microcystaceae. However, the available molecular data for most of the extant species were scarce, and notably, its type species, <i>Me. tranquilla</i>, lacked contemporary distributional and molecular data. In the present study, we sampled multiple localities to capture the potential morphological and genetic variability within <i>Merismopedia</i>. Using culture-dependent and -independent methods, we collected an extensive dataset of 16S rRNA gene and 16S–23S ITS rRNA region sequences. This dataset included the type locality of <i>Me. tranquilla</i>, from which we successfully recovered morphologically authentic isolates and established an epitype. Subsequent phylogenetic analyses of <i>Merismopedia-</i> and <i>Microcrocis</i>-like taxa revealed the placement of <i>Merismopedia</i> sensu stricto within the Geminocystaceae, herein reinstated to Merismopediaceae and further confirmed extensive polyphyly. Based on these findings, we propose two new genera (<i>Neomerismopedia</i> and <i>Planktopedia</i>), and a new species, <i>Me. sepulta</i>. Additionally, we transfer <i>Microcrocis gigas</i> to <i>Merismopedia</i>. We proposed the provisional transfer of <i>Me. hyalina</i> to <i>Synechocystis</i>; however, further revision is needed. These taxonomic revisions provide a foundation for a comprehensive reevaluation of this widespread and morphologically diverse group of Cyanobacteria.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"974-1001"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of ultrasound-assisted extraction of phenolic compounds from Sargassum carpophyllum using response surface methodology","authors":"Xin Liu,&nbsp;Qunjian Yin,&nbsp;Xuyang Chen,&nbsp;Enge Xie,&nbsp;Feng Jiang,&nbsp;Ying Liu","doi":"10.1111/jpy.70175","DOIUrl":"10.1111/jpy.70175","url":null,"abstract":"<p><i>Sargassum</i> spp. has been used in traditional Chinese medicine for nearly 2000 years to treat a variety of diseases due to its pharmacologically active components such as phenolics. In this work, response surface methodology (RSM) was employed to optimize the ultrasound-assisted extraction (UAE) procedures to obtain phenolic compounds from <i>Sargassum carpophyllum</i>, a brown alga widely growing on the coastline of Asian countries. The effects of four extraction variables including ethanol concentration (EtOH%) in the solvent, liquid–solid ratio, ultrasonic power, and duty cycle ratio (DCR) were evaluated using a central composite design (CCD), with the total phenol content (TPC) and antioxidant activity as the two responses. Two quadratic regression models were generated, and the optimum extraction conditions for the highest TPC and antioxidant activity were 50% ethanol, a 40 mL · g⁻¹ of liquid–solid ratio, 260 W, and a 5/6 DCR. The corresponding predicted TPC and antioxidant activity were 2.884 mg phloroglucinol (PHG) · g⁻¹ algae and 3.188 mg Trolox · g⁻¹ algae, respectively, which were in close agreement with the experimental validation values of 2.907 mg PHG · g⁻¹ algae and 3.267 mg Trolox · g⁻¹ algae, respectively. The results suggested that the two regression models could sufficiently describe the UAE procedure and optimize the TPC and antioxidant activity. Results of the Fourier Transform Infrared Spectrometer (FTIR) spectra revealed that phenolic compounds were the major composition of the optimized extract. This work provides essential knowledge in phenolic compounds extraction from <i>S. carpophyllum</i> through mathematical modeling and could be a useful reference for developing upscale UAE procedures.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"855-867"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147963969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}