Journal of Phycology最新文献_第6页

Seasonality and interannual stability in the population genetic structure of Batrachospermum gelatinosum (Rhodophyta) 红藻种群遗传结构的季节性和年际稳定性。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-02-15 DOI: 10.1111/jpy.13539

Sarah Shainker-Connelly, Solenn Stoeckel, Morgan L. Vis, Roseanna M. Crowell, Stacy A. Krueger-Hadfield

{"title":"Seasonality and interannual stability in the population genetic structure of Batrachospermum gelatinosum (Rhodophyta)","authors":"Sarah Shainker-Connelly, Solenn Stoeckel, Morgan L. Vis, Roseanna M. Crowell, Stacy A. Krueger-Hadfield","doi":"10.1111/jpy.13539","DOIUrl":"10.1111/jpy.13539","url":null,"abstract":"Temporal population genetic studies have investigated evolutionary processes, but few have characterized reproductive system variation. Yet, temporal sampling may improve our understanding of reproductive system evolution through the assessment of the relative rates of selfing, outcrossing, and clonality. In this study, we focused on the monoicous, haploid-diploid freshwater red alga Batrachospermum gelatinosum. This species has a perennial, microscopic diploid phase (chantransia) that produces an ephemeral, macroscopic haploid phase (gametophyte). Recent work focusing on single-time point genotyping suggested high rates of intragametophytic selfing, although there was variation among sites. We expand on this work by genotyping 191 gametophytes sampled from four sites that had reproductive system variation based on single-snapshot genotyping. For this study, we sampled at multiple time points within and among years. Results from intra-annual data suggested shifts in gametophytic genotypes throughout the season. We hypothesize that this pattern is likely due to the seasonality of the life cycle and the timing of meiosis among the chantransia. Interannual patterns were characterized by consistent genotypic and genetic composition, indicating stability in the prevailing reproductive system through time. Yet, our study identified limits by which available theoretical predictions and analytical tools can resolve reproductive system variation using haploid data. There is a need to develop new analytical tools to understand the evolution of sex by expanding our ability to characterize the spatiotemporal variation in reproductive systems across diverse life cycles.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 1","pages":"172-193"},"PeriodicalIF":2.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13539","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Algal perspectives: The algal phyla 藻类视角：藻类门。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-02-14 DOI: 10.1111/jpy.13546

Charles F. Delwiche

{"title":"Algal perspectives: The algal phyla","authors":"Charles F. Delwiche","doi":"10.1111/jpy.13546","DOIUrl":"10.1111/jpy.13546","url":null,"abstract":"What are algae? United States Supreme Court Justice Potter Stewart said of pornography, “I shall not today attempt further to define [it]… but I know it when I see it” (Jacobellis v. Ohio, 1964). Something similar could be said of algae. They are not one kind of thing but are a diverse assemblage of (mostly) oxygenic phototrophs, excluding embryophytes (which wags call the “drier algae”). The term is a form classification, not a phylogenetic one, and yet can be useful. There are commonalities of gross appearance, habitat, ecological role, and economic import or potential. Nonetheless, it can be a challenge to understand the full diversity of organisms within our purview as phycologists (algologists), and there are far more algae to know than there are scientists studying them (Figure 1).In his 2012 and 2024 Perspectives, Guiry identified 14 phyla and 63 classes of algae and noted that ongoing discovery continues to expand the list (Guiry, 2012, 2024). The 2012 article has received 8151 full-text views and 696 citations, and the 2024 update has already had 5586 full-text views. This high level of engagement illustrates the hunger for accurate, authoritative, and digestible fundamental information about these organisms. To respond to that interest, I am working with the Journal of Phycology to launch a new series of Perspectives. Each will consist of a short synopsis of a single phylum or class by a leading scholar. They will be intended to be accessible to a broad scientific audience but still be interesting to experts. Of course, these articles will have to be as diverse as the lineages that they are describing, and we anticipate that over time the scope of the series will grow and change. It is our hope that when compiled, these Perspectives will serve as an introduction to the current state of knowledge of algae and provide inspiration for the work that lies ahead.CFD envisioned and wrote the manuscript, took all of the photographs and micrographs in the figure, and prepared the figure and caption.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 1","pages":"1-2"},"PeriodicalIF":2.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13546","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three reference genomes for freshwater diatom ecology and evolution 淡水硅藻生态学和进化的三个参考基因组。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-02-10 DOI: 10.1111/jpy.13545

Wade R. Roberts, Andrew J. Alverson

{"title":"Three reference genomes for freshwater diatom ecology and evolution","authors":"Wade R. Roberts, Andrew J. Alverson","doi":"10.1111/jpy.13545","DOIUrl":"10.1111/jpy.13545","url":null,"abstract":"Diatoms are an important component of marine and freshwater ecosystems. Although the majority of described diatom species live in freshwater systems, genome sequencing efforts have focused primarily on marine species. Genomic resources for freshwater species have the potential to improve our understanding of diatom ecology and evolution, particularly in the context of major environmental shifts. We used long- and short-read sequencing platforms to assemble reference genomes for three freshwater diatom species, all in the order Thalalassiosirales, which are abundant in the plankton of oceans, lakes, reservoirs, and rivers worldwide. We targeted three species that cover the breadth of phylogenetic diversity in the cyclostephanoid clade of Thalassiosirales: Cyclostephanos tholiformis (JALLPB020000000), Discostella pseudostelligera (JALLBG020000000), and Praestephanos triporus (JALLAZ020000000). The reference genome for D. pseudostelligera was considerably smaller (39 Mb) than those of both P. triporus (73 Mb) and C. tholiformis (177 Mb). Long-read sequencing allowed for the assembly of scaffold-level genomes, including regions rich in repetitive DNA. Compared to short-read assemblies, long-read assemblies increased the contig N50 length as much as 37-fold and reduced the number of contigs by more than 88%. Transcriptome-guided annotation of the protein-coding genes identified between 10,000 and 12,000 genes. This work provides further demonstration of the value of long-read sequencing and provides novel genomic resources for understanding the ecology and evolution of freshwater diatoms.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 2","pages":"267-274"},"PeriodicalIF":2.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13545","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking phenotypic variation to patterns of genetic isolation along a speciation continuum 将表型变异与沿物种形成连续体的遗传隔离模式联系起来。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-02-07 DOI: 10.1111/jpy.13529

Sabrina Heiser, Charles D. Amsler, Andrew J. Shilling, Hazel M. Higginbotham, Margaret O. Amsler, Solenn Stoeckel, James B. McClintock, Bill J. Baker, Stacy A. Krueger-Hadfield

{"title":"Linking phenotypic variation to patterns of genetic isolation along a speciation continuum","authors":"Sabrina Heiser, Charles D. Amsler, Andrew J. Shilling, Hazel M. Higginbotham, Margaret O. Amsler, Solenn Stoeckel, James B. McClintock, Bill J. Baker, Stacy A. Krueger-Hadfield","doi":"10.1111/jpy.13529","DOIUrl":"10.1111/jpy.13529","url":null,"abstract":"Investigating taxa at varying stages of divergence can shed light on the evolutionary forces that lead to reproductive isolation and eventual speciation. The forces promoting isolation vary in space and time, which makes it difficult to reconstruct the trajectory that resulted in the divergence observed among species today. The red macroalgal genus Plocamium is known worldwide for its cryptic genetic and chemical diversity. Previous work on the genus Plocamium in Antarctica observed two haplotypes along the western Antarctic Peninsula that have been treated as the same species. Using 10 microsatellite loci, we observed that these two haplotypes correspond to two highly divergent, co-occurring genetic entities in Antarctic Plocamium, which are located within close vicinity of each other at the same sites. The morphology of the reproductive structures, a feature commonly used to identify cryptic species in Plocamium, as well as the timing of reproduction, differed significantly between the two genetic entities. Altogether, this suggests that two Antarctic Plocamium species exist on the western Antarctic Peninsula. We observed evidence for high levels of selfing in both genetic entities, which likely exacerbated the lack of gene flow between them. In addition, we identified concomitant chemodiversity that generates compelling evidence of early evolutionary divergence within one of these entities. This chemodiversity has ecological consequences for its main grazer, which alludes to one putative evolutionary driver of divergence. Antarctic Plocamium spp. provide a promising model system for investigating the eco-evolutionary forces that initiate and maintain species boundaries.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 1","pages":"3-19"},"PeriodicalIF":2.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring cyanobacteria from diverse habitats of the Konkan region of India, unveiling novel species of the genera Desikacharya, Pseudoaliinostoc, and Chlorogloeopsis using a polyphasic approach 探索来自印度康坎地区不同栖息地的蓝藻，使用多相方法揭示Desikacharya， Pseudoaliinostoc和Chlorogloeopsis属的新种。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-01-24 DOI: 10.1111/jpy.13542

Harsh Pant, Naresh Kumar, Sagarika Pal, Prashant Singh

{"title":"Exploring cyanobacteria from diverse habitats of the Konkan region of India, unveiling novel species of the genera Desikacharya, Pseudoaliinostoc, and Chlorogloeopsis using a polyphasic approach","authors":"Harsh Pant, Naresh Kumar, Sagarika Pal, Prashant Singh","doi":"10.1111/jpy.13542","DOIUrl":"10.1111/jpy.13542","url":null,"abstract":"The Indian subcontinent has emerged as a natural habitat to several cyanobacterial taxa which have been explored and described in the past few years using a polyphasic approach. Various new genera and species of Nostoc morphotypes, heteropolar unbranched as well as branched heterocytous cyanobacteria, have been described from various parts of India such as the central mainland, temperate hill stations of extreme northern India, and the biodiversity hotspots of northeast India. Konkan, a small strip of land bounded by Arabian sea on the west and Sahyadri mountains on the east, has various habitats such as coastal beds, old monuments, freshwater lakes, and rivers; however, this region has been less charted in modern cyanobacterial systematics, relative to others. The region has a tropical climate with heavy monsoon showers owing to its location on the windward side of the northern Western Ghats, a global biodiversity hotspot. Through this study, several districts of the Konkan region of Maharashtra and Goa were explored for cyanobacterial diversity and evaluated through a polyphasic approach with three novel species of the genus Desikacharya, two novel species of the genus Pseudoaliinostoc and one new species of the monotypic genus Chlorogloeopsis being described in accordance with the International Code of Nomenclature for algae, fungi and plants (ICN).","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 1","pages":"194-217"},"PeriodicalIF":2.8,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Function of N-acetyltransferase in the biotransformation of aniline in the green alga Chlamydomonas reinhardtii n -乙酰转移酶在莱茵衣藻苯胺生物转化中的作用。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-01-23 DOI: 10.1111/jpy.13543

Taoxing Dong, Xiaofeng Liu, Yingli Yang, Jun Xiong, Xuedong Wang, Shao Yang

{"title":"Function of N-acetyltransferase in the biotransformation of aniline in the green alga Chlamydomonas reinhardtii","authors":"Taoxing Dong, Xiaofeng Liu, Yingli Yang, Jun Xiong, Xuedong Wang, Shao Yang","doi":"10.1111/jpy.13543","DOIUrl":"10.1111/jpy.13543","url":null,"abstract":"Phytoplankton plays a crucial role in the fate of pollutants in aquatic ecosystems by biotransformation and bioaccumulation. Aniline was listed in priority pollutants due to its toxicity and widespread distribution in the aquatic environment. This study focused on investigating the capacity and mechanism of eukaryotic alga Chlamydomonas reinhardtii in transforming aniline. Results showed that the total removal percentage of aniline was 56% within 8 days at an initial concentration of 10 mg · L−1. The percentage of the biotransformation by C. reinhardtii was 23.4%. The biotransformation product was identified as acetanilide, indicating that acetylation was the primary biotransformation pathway. To reveal the key enzyme of the biotransformation process, the N-acetyltransferase (NAT) gene was cloned from the C. reinhardtii genome, and the NAT protein was obtained through heterologous expression. Aniline was significantly transformed by the purified NAT protein in vitro, and the product was also acetanilide. The characteristics of C. reinhardtii NAT in biotransformation of aniline was analyzed by bioinformatics methods. The binding sites in C. reinhardtii NAT for ligands (aniline and acetyl-CoA) were identified. Three highly reserved valine residues (Glu85-Asp86-Val87-Val88-Val89) as well as GLU131 and Cys122 were the indispensable amino acid residues for the catalysis from aniline to acetanilide. These results demonstrated the capacity of C. reinhardtii in the biotransformation of aniline, and the transformation process was primarily through N-acetylation of aniline to acetanilide catalyzed by NAT enzyme. This study provides novel insights into the biotransformation mechanism of aniline in eukaryotic green alga C. reinhardtii, facilitating the evaluation of the fate of aniline within aquatic ecosystems.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 1","pages":"231-240"},"PeriodicalIF":2.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asynchronous shifts in the demographics of two wave-swept kelp species (Laminariales) after nearly four decades 近四十年来，两种被海浪席卷的海带物种（海带）的人口结构发生了不同步的变化。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-01-23 DOI: 10.1111/jpy.13544

Samuel Starko, Alyssa Allchurch, Christopher Neufeld

{"title":"Asynchronous shifts in the demographics of two wave-swept kelp species (Laminariales) after nearly four decades","authors":"Samuel Starko, Alyssa Allchurch, Christopher Neufeld","doi":"10.1111/jpy.13544","DOIUrl":"10.1111/jpy.13544","url":null,"abstract":"Kelp forests are among the most abundant and productive marine ecosystems but are under threat from climate change and other anthropogenic stressors. Although knowledge is growing about how the abundance and distribution of kelp forests are changing, much less is known about the “non-lethal” effects that global change is having on the performance and health of kelp populations in areas where they persist. Here we assessed the age distribution of two common stipitate kelp species, Laminaria setchelli and Pterygophora californica, at Wizard Islet in Barkley Sound, British Columbia, Canada, and compared these data to historical demographic data collected by De Wreede (1984) and Klinger and DeWreede (1988) from the same site between 1981 and 1983. We observed that L. setchelli populations in 2020 were younger and less evenly aged than the same populations sampled nearly four decades prior, while the P. californica population was composed of older individuals on average than at the historical time point. Although the drivers of these demographic changes remain unclear, Barkley Sound has experienced substantial changes in the physical and biological environment over the past decade that could be responsible for these patterns. Given that the size of an individual and its probability of reproduction increases with age, shifting demographics may impact the reproductive output of each population, potentially altering the competitive relationships between co-occurring species. Changes in size distribution may also influence ecosystem-level processes such as habitat complexity or productivity.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 1","pages":"250-254"},"PeriodicalIF":2.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13544","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Bryocladia scopulorum complex from the Southwestern Atlantic Ocean with the description of three new species of Bryocladia (Rhodomelaceae, Rhodophyta) 西南大西洋东莨菪苔藓虫复合体的揭示与3个苔藓虫新种的描述（红藓科，红藓门）。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-01-15 DOI: 10.1111/jpy.13535

Jhullyrson Osman Ferreira de Brito, Valéria Cassano

{"title":"Unraveling the Bryocladia scopulorum complex from the Southwestern Atlantic Ocean with the description of three new species of Bryocladia (Rhodomelaceae, Rhodophyta)","authors":"Jhullyrson Osman Ferreira de Brito, Valéria Cassano","doi":"10.1111/jpy.13535","DOIUrl":"10.1111/jpy.13535","url":null,"abstract":"The filamentous red algal genus Bryocladia was recently deeply revised based on molecular and morphological data. However, data from the Southwestern Atlantic Ocean are scarce. Here, we provide a phylogenetic study of Bryocladia representatives from the Brazilian coast with new additions to the genus. Our samples were collected from the north to southeastern Brazilian coast in marine and estuarine areas. We carried out a morphological and molecular study based on COI-5P and rbcL gene sequences. Our phylogenetic analyses confirmed the presence of seven species on the Brazilian coast, four of which were previously known: B. cuspidata, B. subtilissima, B. thyrsigera, and B. villum. Three new species are described here, B. itaipensis sp. nov., B. oceanica sp. nov., and B. mucuripensis sp. nov., all morphologically included in the B. scopulorum complex. Bryocladia subtilissima was observed only in Brazilian estuarine areas. However, it was split into two subclades, which require further investigation. The three new species expand the known diversity of the B. scopulorum complex from 12 to 15 species. Bryocladia itaipensis is closely related to B. canariensis and B. caespitosa, whereas B. oceanica and B. mucuripensis formed distinct lineages with unsolved relationships. All new species are restricted to their type localities in Brazilian waters, reinforcing the need for further study of tiny species, especially from the tribes Polysiphonieae and Streblocladieae.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 1","pages":"61-76"},"PeriodicalIF":2.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional consistency of macroalgae across a sea ice cover gradient along the Western Antarctic Peninsula 南极半岛西部海冰覆盖梯度上大型藻类的营养一致性。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-01-12 DOI: 10.1111/jpy.13541

Hannah E. Oswalt, Margaret O. Amsler, Charles D. Amsler, Katrin Iken, James B. McClintock, Andrew G. Klein, Aaron W. E. Galloway

{"title":"Nutritional consistency of macroalgae across a sea ice cover gradient along the Western Antarctic Peninsula","authors":"Hannah E. Oswalt, Margaret O. Amsler, Charles D. Amsler, Katrin Iken, James B. McClintock, Andrew G. Klein, Aaron W. E. Galloway","doi":"10.1111/jpy.13541","DOIUrl":"10.1111/jpy.13541","url":null,"abstract":"Sea ice can profoundly influence photosynthetic organisms by altering subsurface irradiance, but it is susceptible to changes in the climate. The patterns and timing of sea ice cover can vary on a monthly to annual timescale in small sub-regions of the Western Antarctic Peninsula (WAP). During the latter part of the 20th century, sea ice coverage significantly decreased in the WAP, a trend that aligns with warming in this area. Macroalgal biochemical components are impacted by light availability, often showing a close relationship between photosynthesis and biochemical compositions. We used satellite imagery of annual sea ice duration and extent as well as water turbidity during ice-free periods to identify 14 study sites that differed dramatically in sea ice coverage but were similar in terms of turbidity along the central WAP between 68° S and 64° S. The common macroalgal species Desmarestia menziesii, Himantothallus grandifolius, Sarcopeltis antarctica, and Iridaea sp. were collected by scuba divers between 5 m and 35 m depth at each site where they occurred, for later biochemical analyses. Overall percentages of major biochemical components as well as carbon and nitrogen percentages and C:N were determined and correlated with four different sea ice indices. Surprisingly, most of the chemical components were not significantly correlated with sea ice cover. The few significant correlations varied between species and chemical components. This indicates that although patterns of sea ice coverage have major implications for macroalgal abundance, on a per-biomass basis, sea ice coverage does not impact the nutritional contributions of macroalgae to food webs.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 1","pages":"218-230"},"PeriodicalIF":2.8,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural analysis of a membrane-associated delta-6 fatty acid desaturase from Prorocentrum micans micans原中膜相关δ -6脂肪酸去饱和酶的结构分析。

IF 2.8 3区生物学

Journal of Phycology Pub Date : 2025-01-07 DOI: 10.1111/jpy.13540

Zhihong Du, Kaiwen Sun, Shangyi Xu, Xiao Qiu

{"title":"Structural analysis of a membrane-associated delta-6 fatty acid desaturase from Prorocentrum micans","authors":"Zhihong Du, Kaiwen Sun, Shangyi Xu, Xiao Qiu","doi":"10.1111/jpy.13540","DOIUrl":"10.1111/jpy.13540","url":null,"abstract":"Delta-6 fatty acid desaturases, which play key roles in the biosynthesis of polyunsaturated fatty acids (PUFAs), are membrane-associated enzymes that present significant challenges for isolation and purification, complicating their structural characterization. Here we report the identification and structure–function analysis of a novel Δ6 fatty acid desaturase (PmD6) from the marine alga Prorocentrum micans with substrate preference to α-linolenic acid (18:3n-3). Structural modeling revealed a mushroom-like structure of PmD6 formed by four transmembrane α-helices as a stem and three cytoplasmic domains as a cap. Structural alignment identified several key residues positioned around the substrate tunnel and catalytic center in PmD6. Functional analysis of these residues by site-directed mutagenesis showed that Tyr226, Trp235, Phe345, and Ser349, facing the middle region of the substrate tunnel of PmD6, played critical roles in defining the structure for acceptance of substrates. Thr200, Leu391, and Met389, surrounding the end of the substrate tunnel, had roles in interaction with the methyl end of substrates. Asp255, close to a metal iron in the catalytic center, was essential for catalytic reaction by supporting the regional structure. These results have provided mechanistic insights into the structure–function relationship of membrane-bound front-end fatty acid desaturases.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"61 1","pages":"161-171"},"PeriodicalIF":2.8,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0