Journal of Phycology最新文献

{"title":"Impact of the invasive diatom species Cymbella janischii on riverine microbial biofilm communities and a potential role of bacterially produced zeatin","authors":"Eldrin D. L. R. Arguelles,&nbsp;Kana Mugikura,&nbsp;Shinya Sato","doi":"10.1111/jpy.70195","DOIUrl":"10.1111/jpy.70195","url":null,"abstract":"<p>The diatom <i>Cymbella janischii</i> is an invasive species in Japan, causing nuisance blooms by forming thick mats in rivers. To date, there are no documented studies on the microbiome associations in <i>C. janischii</i> mats or the processes that drive bloom formation. This study used metabarcoding of diatoms, bacteria, and fungi to identify key species and assess the effects of <i>C. janischii</i> blooms on the benthic microbial communities. <i>C. janischii</i> blooms reduced diatom and bacterial species diversity, while fungal diversity remained stable. In addition, the diatom <i>Nitzschia paleacea</i> and the bacterium <i>Flavobacterium</i> sp. were observed to co-occur and vary in abundance, indicating a possible ecological link that may affect mat structure or function. Metagenomic predictions of bacterial functions showed that compared to benthic stones without visible <i>C. janischii</i> mats, mat-associated bacteria had enriched pathways related to the metabolism of carbohydrates, nucleotides, and amino acids, along with zeatin biosynthesis. Zeatin is a cytokinin phytohormone that stimulates plant growth and development. In vitro exposure of <i>C. janischii</i> to varying zeatin concentrations confirmed its growth-promoting effects, inducing cell proliferation and stalk formation. This study shows that zeatin stimulates the growth of <i>C. janischii</i>. The findings of this study provide new insights into microbiome diversity, identifying key taxa associated with <i>C. janischii</i> mats to help better understand bloom formation.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 4","pages":"1221-1239"},"PeriodicalIF":4.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148352249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lines of dehiscence as a biomechanical strategy for controlling damage during blade development in the bull kelp Nereocystis luetkeana","authors":"Alana Breitkreutz,&nbsp;Patrick T. Martone","doi":"10.1111/jpy.70198","DOIUrl":"10.1111/jpy.70198","url":null,"abstract":"<p>Although tearing is most often considered mechanical damage, it plays a central role in blade development in the bull kelp <i>Nereocystis luetkeana</i>. Although developmental tearing has been described in other macrophytes, the presence of a line of dehiscence that directs fracture apart from reproduction or abscission remains rare and poorly understood. This study shows that lines of dehiscence in <i>Nereocystis</i> promote clean, longitudinal blade splits by reducing tearing strength and facilitating crack propagation in flow. These effects are especially pronounced in juvenile blades, in which anisotropic tearing properties have not yet fully developed. By combining structural weakening with flow-driven crack extension, <i>Nereocystis</i> generates new blades through a mixed strategy of physiological patterning and passive fracture, revealing a previously underrecognized biomechanical strategy for controlling damage and adjusting morphology.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 4","pages":"1356-1366"},"PeriodicalIF":4.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70198","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148411683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redescription and neotypification of Alexandrium minutum Halim—the type species of Alexandrium—from its type locality in Alexandria, Egypt, with insights into ribotype biogeography and toxicity","authors":"Shuning Huang,&nbsp;Haifeng Gu,&nbsp;Bernd Krock,&nbsp;Nihal G. Shams El-Din,&nbsp;Øjvind Moestrup,&nbsp;Ewenn Cochet,&nbsp;Kenneth Mertens","doi":"10.1111/jpy.70190","DOIUrl":"10.1111/jpy.70190","url":null,"abstract":"<p><i>Alexandrium minutum</i> Halim, the generitype species of the toxigenic <i>Alexandrium,</i> was described from a 1958 bloom in the Eastern Harbour, Alexandria (Egypt), but has never been re-examined with modern methods from the type locality. Here, we establish a monoclonal strain (TIO1796) by cyst germination from Alexandria's port sediment and a second strain (TIO1816) from plankton samples from the Gulf of Suez. Cell morphology of both cultured strains and plankton samples was examined using scanning electron and light microscopy. The plate formula of <i>A. minutum</i> was identified as Po, *4′, 6′′, 6C, 9S, 5′′′, 2p, 1′′′′. Our results confirmed the consistent presence of a ventral pore and a variable connection between Po and 1′. Molecular phylogenetic analyses based on LSU rRNA gene and ITS rRNA region sequences revealed four ribotypes (A–D) of <i>A. minutum</i>. The LSU rRNA gene and ITS rRNA region sequences of both strains were identical and belong to the widespread ribotype A (“Global/Mediterranean clade”). The other ribotypes are restricted to the Asia-Pacific region. Strain TIO1796 (type locality) was non-toxic regarding paralytic shellfish toxin (PST &lt; 0.024 pg · cell⁻¹), whereas TIO1816 produced GTX1/4-dominated toxins (0.48 pg PST · cell⁻¹). These results (i) anchor the name <i>A. minutum</i> to ribotype A via neotypification of strain TIO1796, (ii) document a PST-producing <i>A. minutum</i> in the Red Sea, and (iii) provide evidence supporting that <i>A. minutum</i> constitutes a cryptic species complex analogous to the <i>A. tamarense</i> species complex. The historical decline of <i>A. minutum</i> blooms in Alexandria since the 1990s correlates with near-absent cyst banks in recent sediments.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 4","pages":"1311-1326"},"PeriodicalIF":4.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148258493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chloroplast and mitochondrial genomes of Sphaerosorus coelastroides Pascher (Xanthophyceae) from Central Appalachia, Clinch River, Virginia, United States","authors":"Gavin S. Collins,&nbsp;Robin A. Matthews,&nbsp;A. Bruce Cahoon","doi":"10.1111/jpy.70185","DOIUrl":"10.1111/jpy.70185","url":null,"abstract":"<p>This report describes the isolation and culture of <i>Sphaerosorus coelastroides</i> from the plankton of a freshwater river in the central Appalachian mountain chain. This alga was first observed in a dry freshwater riverbed in Central Europe in 1908 and, since then, has been included in Xanthophyceae surveys from nearly all continents with temperate climates. Differential interference microscopic analysis of our isolate revealed cell sizes ranging from 10.2 to 19 μm in diameter occurring in groups of two to 32 cells with a mucilage layer that changed thickness depending upon the phase of the life cycle. Chloroplasts were light green, and confocal fluorescence microscopy revealed three or more per cell. Genetic markers were sequenced, annotated, and deposited into GenBank, and include the 18S nuclear rRNA gene (PX102079) and completed chloroplast (PV826133) and mitochondrial (PV826132) genomes. Phylogenetic analyses using 18S and <i>rbc</i>L gene sequences revealed that this isolate was closely related to <i>S. composita</i>, the only other known species from this genus. This isolate was deposited into the UTEX Culture Collection of Algae, making it the only strain of this alga available for phycological study at this time.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 4","pages":"1185-1192"},"PeriodicalIF":4.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental constraints on freshwater red algae in Estonian streams: Insights from long-term observations and machine-learning models","authors":"Kai Piirsoo,&nbsp;Sirje Vilbaste,&nbsp;Peeter Pall,&nbsp;Kalle Olli","doi":"10.1111/jpy.70188","DOIUrl":"10.1111/jpy.70188","url":null,"abstract":"<p>The division Rhodophyta (red algae) includes an inconspicuous and often overlooked component of benthic freshwater biota. In many cases, freshwater red algae are considered indicators of good ecological status, yet their environmental preferences remain poorly understood. Species richness is low globally, and the distribution of many taxa remains poorly known. Here, we report the occurrence of 12 taxa of benthic red algae in 272 Estonian streams, based on 734 sampling sites and 1263 sampling occasions. The aim of this study is to identify the key environmental drivers of freshwater red algae occurrence and to evaluate their potential as indicators of water quality. Red algae were recorded in 20% of the samples. The influence of 37 environmental variables on the occurrence of red algae was assessed using machine-learning algorithms (random forest and gradient boosting trees). Hydrological and hydrochemical variables were by far the most important variable groups, followed by bottom substrate, watershed land cover and land use, and geological bedrock type. Among the hydrochemical variables, biological oxygen demand was the single most important predictor, indicating a high sensitivity of freshwater red algae to labile organic pollution. In contrast, the effect sizes of mineral and total nutrients were lower and more nuanced, suggesting that freshwater red algae are not particularly sensitive indicators of eutrophication. Some division-level responses may have been obscured by contrasting species-specific patterns. Species-level effects were often difficult to verify using machine-learning approaches due to low occurrence frequencies.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 4","pages":"1169-1184"},"PeriodicalIF":4.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148308708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative taxonomy of Harveylithon (Corallinales, Rhodophyta) reveals three new species from Korea and expanded diversity in the northwestern Pacific","authors":"Gwan Woung Kim,&nbsp;So Young Jeong,&nbsp;Boo Yeon Won,&nbsp;Suzanne Fredericq,&nbsp;Tae Oh Cho","doi":"10.1111/jpy.70202","DOIUrl":"10.1111/jpy.70202","url":null,"abstract":"<p>Non-geniculate coralline genus <i>Harveylithon</i> includes 12 species worldwide. In this study, we propose three new species, <i>Ha. koreanum</i> sp. nov., <i>Ha. longiforme</i> sp. nov., and <i>Ha. planiforme</i> sp. nov., based on integrative molecular and morphological analyses. These three new species share the diagnostic morphological characters of <i>Harveylithon</i>, including a monomerous, non-coaxial thallus construction with perithallial cells oriented perpendicularly to the surface; a hypothallial layer composed of rectangular cells aligned parallel to the substratum; and the presence of trichocytes occurring singly, in pairs, or in clusters. Our phylogenetic analyses of a concatenated four-gene data set (COI + <i>rbc</i>L + <i>psb</i>A + SSU rRNA) clearly resolved the three new species within <i>Harveylithon</i>, each forming a well-supported and distinct lineage. The topologies of the individual-gene phylogenies based on the <i>psb</i>A and the SSU rRNA genes were largely congruent and supported these relationships, whereas phylogenetic trees based on the <i>rbc</i>L and COI genes did not recover <i>Harveylithon</i> as monophyletic, with <i>Dawsoniolithon</i> species nested within the <i>Harveylithon</i> clade. Interspecific sequence divergences between new species and their congeners were 6.3%–13.7% for the COI gene, 0.8%–9.8% for the <i>psb</i>A gene, and 1.8%–14.6% for the <i>rbc</i>L gene. <i>Harveylithon koreanum</i> sp. nov. and <i>Ha. planiforme</i> sp. nov. are currently known from only Jeju Island in Korea, whereas <i>Ha. longiforme</i> sp. nov. was identified from both Jeju Island, Korea, and India, indicating that it may have a broad Indo-Pacific distribution. In addition, we newly generated the <i>psb</i>A gene sequence from the generitype, <i>Ha. rupestre</i> (TRH A3-149) and incorporated it into our molecular data set to stabilize the phylogenetic circumscription of the genus.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 4","pages":"1392-1411"},"PeriodicalIF":4.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70202","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting the life cycle of Margalefidinium polykrikoides Group III","authors":"Eduardo Pérez-Vega,&nbsp;Kenneth N. Mertens,&nbsp;Pjotr Meyvisch,&nbsp;Margaret R. Mulholland","doi":"10.1111/jpy.70201","DOIUrl":"10.1111/jpy.70201","url":null,"abstract":"<p>Dinoflagellates produce cysts as a strategy to withstand environmental stressors, with nutrient depletion generally considered a key trigger for cyst production. Resting cysts are thick-walled, typically composed of one to several layers, and characterized by a prolonged dormancy period. In contrast, pellicle cysts possess a thin, single wall and exhibit no dormancy or a markedly shorter dormancy than resting cysts of the same species. <i>Margalefidinium polykrikoides</i> produces pellicle and resting cysts, whereas its congener, <i>M. fulvescens</i>, has been shown to produce pumpkin-like structures. Using phase-contrast microscopy, time-lapse microscopy, FlowCam, and attenuated total reflection Fourier transform infrared microspectroscopy (ATR μ-FTIR), we investigated the environmental triggers of sac production, the role of the sacs in the life cycle of the <i>M. polykrikoides</i> American/Malaysian ribotype (Group III) strain, and the chemical composition of the sacs. The sacs observed here differed from pellicle cysts described in <i>M. polykrikoides</i> Group III NY and Group I Korea strains but were indistinguishable from the pumpkin-like structures of <i>M. fulvescens</i>. Phosphate depletion of the culture medium was determined as the main trigger for sac production. Sacs were produced through both sexual and asexual processes. We report the presence of sac transporters—diploid cells that facilitate sac movement. The ATR μ-FTIR data indicated that the sacs were primarily composed of sulfated polysaccharides, closely resembling the extracellular polymeric substances produced by <i>M. polykrikoides</i> (Japan strain) and by <i>Gymnodinium</i> sp. Based on their single thin wall and absence of dormancy, these sacs are considered pellicle cysts.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 4","pages":"1367-1382"},"PeriodicalIF":4.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148396998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient disturbance in a shallow aquaculture pond impacts Microcystis gene expression but does not impact bacterial community function during bloom conditions","authors":"Matthew F. Gladfelter,&nbsp;Hunter R. Baylous,&nbsp;Alan E. Wilson,&nbsp;Morgan M. Steffen","doi":"10.1111/jpy.70197","DOIUrl":"10.1111/jpy.70197","url":null,"abstract":"<p>Cyanobacterial harmful algal blooms (cHABs) are worldwide issues. Reduced nitrogen forms (ammonium and urea) have recently been measured in freshwater systems at concentrations not previously recorded. These reduced nitrogen forms have been shown to favor the proliferation of harmful cyanobacteria. These blooms are comprised of a diverse community of microbes that contribute to nutrient cycling and other ecosystem functions. To measure the response of the <i>Microcystis</i> bloom microbiome, a field experiment was conducted to examine the transcriptional responses of <i>Microcystis</i>, a common bloom-forming cyanobacterium, as well as the co-occurring bacteria associated with the bloom. Limnocorrals were fertilized with either nitrate, ammonium, or urea, and samples were collected across a 24-h time series after nutrient additions to track changes in <i>Microcystis</i> gene expression along with bacterial function and composition using metatranscriptomics. <i>Microcystis</i> spp. dominated experimental enclosures throughout the experiment (&gt;70% of total bacterial reads). This stability was also reflected in the community structure and function of the co-occurring bacteria, which had no substantial changes over the 24-h after nutrient additions. Nutrient additions drove immediate differential expression responses for <i>Microcystis</i>, and the response was nitrogen form dependent. Key gene groups including core metabolite-related genes and carbon acquisition genes had pronounced differences among treatments, while toxin-related gene expression (<i>mcy</i>ABCDEFGHIJ) was not impacted by nitrogen treatments. Results support previous lab and field-based experiments that have suggested that reduced nitrogen forms impact cHAB molecular physiology, even during peak bloom and elevated nutrient conditions in shallow systems frequently impacted by agricultural runoff and/or aquacultural input.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 4","pages":"1297-1310"},"PeriodicalIF":4.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.70197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective grazing in the heterotrophic dinoflagellate Oxyrrhis marina: Effects of prey type, size, temperature, and nutrient limitation.","authors":"Dayong Liang, Ying Zhang, Huanxin Li, Jianping Sun, Zeyu Li, Hua Xiang","doi":"10.1111/jpy.70192","DOIUrl":"https://doi.org/10.1111/jpy.70192","url":null,"abstract":"<p><p>Protists are the main consumers of phytoplankton in the marine environment, playing pivotal roles in the carbon cycle and nutrient regeneration. Heterotrophic dinoflagellates, which have been extensively studied, are the linking hubs for microbial food loops and classical food chains. In particular, Oxyrrhis marina has been used as a model predator in laboratory studies. Designed experiments were conducted to explore the effects of biological factors (prey type, particle size) and abiotic factors (temperature, nutrient limitation) on its growth and ingestion. Oxyrrhis marina had the ability to consume various algae, including Chromalveolata, Haptophyta, Chlorophyta, Bacillariophyta, and Ochrophyta. When prey had diameters ranging from 7.4 to 13 μm, O. marina exhibited the highest ingestion rates, growth rates, and cell volume. With increases in temperature, the ingestion rate, growth rate, and productivity of O. marina increased significantly, but the cell volume decreased dramatically. Compared to ingesting phosphorus-restricted prey, ingesting nitrogen-restricted prey significantly increased the ingestion rate, growth rate, and cell volume of O. marina. Nitrogen-restricted (phosphorus-restricted) O. marina preferred to select prey that contained more nitrogen (phosphorus) elements to regulate nutritional imbalance. The purpose of this study is to provide a theoretical foundation for understanding the selective ingestion of the heterotrophic dinoflagellate O. marina and its impact on the community structure of plankton.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148352177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms, metabolic remodeling, and energy reallocation underlying calcification in the coccolithophore Gephyrocapsa huxleyi","authors":"Cong Wang,&nbsp;Yan Liu,&nbsp;Guanjia Lin,&nbsp;Xinyu Deng,&nbsp;Senjie Lin,&nbsp;Nianzhi Jiao","doi":"10.1111/jpy.70170","DOIUrl":"10.1111/jpy.70170","url":null,"abstract":"<p>Coccolithophores play a dual role in the marine carbon cycle, serving as CO₂ sinks through photosynthesis while simultaneously emitting CO₂ via calcification, resulting in uncertainty regarding their net carbon sequestration potential. In addition, their calcite coccoliths (CaCO₃) can increase the carbon export efficiency by functioning as ballasts for organic matter. Although biogeochemically significant, the molecular mechanisms governing calcification and associated metabolic adaptations in coccolithophores remain poorly characterized, impeding accurate predictions of their responses to climate change. Through comparative multiomics analyses of calcified (RCC1266) and noncalcified (PML B92/11) <i>Gephyrocapsa huxleyi</i> strains, as well as chemically induced decalcified and recalcified states, we screened several ion transport genes, which potentially facilitate Ca²⁺ and HCO₃⁻ uptake/transport coupled with H⁺ extrusion during calcification in the calcified strain, along with their associated proteins, including signal molecules and chaperones. Furthermore, an energy-intensive process was observed in calcifying cells, and this process was principally sustained by enhanced photosynthetic efficiency, supplemented by glucose accumulation as an energy reserve and COX6B translational upregulation, providing nocturnal energy. Notably, calcifying cells employed an energy conservation strategy characterized by transcriptional downregulation yet translational maintenance of photosynthesis and carbon metabolism genes while simultaneously upregulating protein biosynthesis and trafficking pathways to probably meet calcification demands, a process potentially facilitated by increased glutamine biosynthesis. Through multiomic technology, our findings provide insights into the molecular adaptations in the calcified coccolithophorid cells, revealing critical physiological trade-offs, carbon metabolism, and energy allocation that can inform predictions of their acclimation capacity under changing oceanic conditions.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":"62 3","pages":"943-958"},"PeriodicalIF":4.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}