Journal of Phycology最新文献

{"title":"Ca2+-mediated reactive oxygen species signaling regulates cell repair after mechanical wounding in the red alga Griffithsia monilis","authors":"Chan Young Hong,&nbsp;Ji Ho Yun,&nbsp;Gwang Hoon Kim","doi":"10.1111/jpy.13476","DOIUrl":"10.1111/jpy.13476","url":null,"abstract":"<p>Mechanical damage to a cell can be fatal, and the cell must reseal its membrane and restore homeostasis to survive. Plant cell repair involves additional steps such as rebuilding vacuoles, rearranging chloroplasts, and remodeling the cell wall. When we pierced a <i>Griffithsia monilis</i> cell with a glass needle, a large amount of intracellular contents was released, but the cell membrane resealed in less than a second. The turgor of the vacuole was quickly restored, and the punctured cell returned to its original shape within an hour. Organelles such as chloroplasts and nuclei migrated to the wound site for 12 h and then dispersed throughout the cell after the wound was covered by a new cell wall. Using fluorescent probes, high levels of reactive oxygen species (ROS) and calcium were detected at the wound site from 3 h after wounding, which disappeared when cell repair was complete. Wounding in a solution containing ROS scavengers inhibited cellular repair, and inhibiting nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity or blocking calcium influx reversibly inhibited cell repair. Oryzalin reversibly inhibited both chloroplast movement and ROS production during cell repair. Our results show that cell repair in <i>G. monilis</i> is regulated by calcium-mediated ROS signaling and that microtubules serve as mechanical effectors.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13476","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted extraction of phenolics from Sargassum carpophyllum – A kinetics study","authors":"Xin Liu,&nbsp;Qunjian Yin,&nbsp;Xuyang Chen,&nbsp;Pengfei Sun,&nbsp;Ying Liu","doi":"10.1111/jpy.13477","DOIUrl":"10.1111/jpy.13477","url":null,"abstract":"<p>The species of the brown macroalgal genus <i>Sargassum</i> are distributed globally and contain many bioactive compounds. In this study, ultrasound-assisted extraction (UAE) was applied to obtain phenolic compounds with strong antioxidant activity from <i>Sargassum carpophyllum</i> collected along the coastline of Weizhou Island in the South China Sea. The influence of different variables such as the solid–liquid ratio (1:5–1:30 g · mL⁻¹), ultrasonic power (160–280 W), duty circle ratio (DCR, 1/3–1/1), and ethanol concentration (30% to ~90%) were studied using a single factor design. The extraction kinetics were investigated using the Peleg model and second-order kinetics model, and the second-order model described the extraction procedure better than the Peleg model. Total phenol content (TPC) values of 3.316, 2.964, 2.741, and 3.665 mg phloroglucinol (PHG) · g⁻¹ algae were achieved at a higher solid–liquid ratio (1:30 g · mL⁻¹), higher ultrasonic power (280 W), a higher DCR (1/1), and a moderate ethanol concentration (50%), respectively. However, a slightly different result was observed in the extract obtained, with total phenol contents (TPC_extract) of 52.99, 65.00, 46.22, and 55.10 mg PHG · g⁻¹ extract and DPPH radical scavenging activity (IC50) of 0.096, 0.066, 0.131, and 0.136 mg extract · mL⁻¹ observed at 50% ethanol, 1:5 g m· mL⁻¹, 2/3 DCR, and 200 W respectively. All variables studied influenced the extraction kinetics by altering the extraction rate and the TPC at equilibrium. As for the bioactivities in the extract, a larger solid–liquid ratio and greater ultrasonic power may not contribute because of their ability to extract non-phenolic components simultaneously, leading to reduced overall bioactivities. The results of the present study provide essential information for future UAE process design and optimization for extracting phenolics from <i>S. carpophyllum</i> through mathematical modeling and could be regarded as important reference for obtaining value-added products from other macroalgae species.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population genomics resolves cryptic species of the ecologically flexible genus Laspinema (Cyanobacteria)","authors":"Petr Dvořák,&nbsp;Svatopluk Skoupý,&nbsp;Hana Jarošová,&nbsp;Kateřina Páleníčková,&nbsp;Aleksandar Stanojković","doi":"10.1111/jpy.13475","DOIUrl":"10.1111/jpy.13475","url":null,"abstract":"<p>Cyanobacterial taxonomy is entering the genomic era, but only a few taxonomic studies have employed population genomics, which provides a framework and a multitude of tools to understand species boundaries. Phylogenomic and population genomic analyses previously suggested that several cryptic lineages emerged within the genus <i>Laspinema</i>. Here, we apply population genomics to define boundaries between these lineages and propose two new cryptic species, <i>Laspinema olomoucense</i> and <i>L. palackyanum</i>. Moreover, we sampled soil and puddles across Central Europe and sequenced the 16S rRNA gene and 16S-23S ITS region of the isolated <i>Laspinema</i> strains. Together with database mining of 16S rRNA gene sequences, we determined that the genus <i>Laspinema</i> has a cosmopolitan distribution and inhabits a wide variety of habitats, including freshwater, saline water, mangroves, soil crusts, soils, puddles, and the human body.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13475","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta)","authors":"Yujie Li,&nbsp;Jiali Yang,&nbsp;Zhenjie Sun,&nbsp;Jianfeng Niu,&nbsp;Guangce Wang","doi":"10.1111/jpy.13474","DOIUrl":"10.1111/jpy.13474","url":null,"abstract":"<p>The northward shift of <i>Pyropia yezoensis</i> aquaculture required the breeding of germplasms with tolerance to the oxidative stress due to the high light conditions of the North Yellow Sea area. The MPV17/PMP22 family proteins were identified as a molecule related to reactive oxygen species (ROS) metabolism. Here, one of the <i>MPV17</i> homolog genes designated as <i>PyM-LP2</i> was selected for functional identification by introducing the encoding sequence region/reverse complementary fragment into the <i>Py. yezoensis</i> genome. Although the photosynthetic activity, the respiratory rate, and the ROS level in wild type (WT) and different gene-transformed algal strains showed similar levels under normal conditions, the overexpression (OE) strain exhibited higher values of photosynthesis, respiration, and reducing equivalents pool size but lower intracellular ROS production under stress conditions compared with the WT. Conversely, all the above parameters showed opposite variation trends in RNAi strain as those in the OE strain. This implied that the PyM-LP2 protein was involved in the mitigation of the oxidative stress. Sequence analysis revealed that this PyM-LP2 protein was assorted to peroxisomes and might serve as a poring channel for transferring malate (Mal) to peroxisomes. By overexpressing <i>PyM-LP2</i>, the transfer of Mal from chloroplasts to peroxisomes was enhanced under stress conditions, which promoted photorespiration and ultimately alleviated excessive reduction of the photosynthetic electron chain. This research lays the groundwork for the breeding of algae with enhanced resistance to oxidative stresses.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Putting together the polyphosphate puzzle for microalgae","authors":"Sonya T. Dyhrman","doi":"10.1111/jpy.13460","DOIUrl":"10.1111/jpy.13460","url":null,"abstract":"","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141300880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic activity of deep living cyanobacteria: Photosynthesis versus respiration","authors":"Tomasz Lenard","doi":"10.1111/jpy.13458","DOIUrl":"10.1111/jpy.13458","url":null,"abstract":"","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141300879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A revision of the genus Wrangelia (Wrangeliaceae, Ceramiales) in Bermuda resolves six new species including W. ryancraigii from the mesophotic zone","authors":"Walter M. Jongbloed,&nbsp;Craig W. Schneider,&nbsp;Christopher E. Lane,&nbsp;Margaret M. Cassidy,&nbsp;Gary W. Saunders","doi":"10.1111/jpy.13466","DOIUrl":"10.1111/jpy.13466","url":null,"abstract":"<p>Four species of the genus <i>Wrangelia</i> are presently known from the western Atlantic Ocean: <i>W. argus</i>, <i>W. bicuspidata</i>, <i>W. penicillata,</i> and <i>W. gordoniae</i>, with the first three historically being reported from Bermuda. Morphological and molecular barcode (COI-5P) and phylogenetic analyses used in this study (SSU, LSU, <i>rbc</i>L) indicated eight species groupings of <i>Wrangelia</i> in Bermuda, excluding two of the historically recognized species, retaining only <i>W. argus</i> while adding seven new species, of which six are formally described. What had been historically reported as <i>W. penicillata</i> from Bermuda was shown to be distinct from Mediterranean Sea specimens (type locality) and was shown to be a mixture of <i>W. hesperia</i> sp. nov. and <i>W. incrassata</i> sp. nov. Along with these two, three other new species (<i>W. laxa</i> sp. nov., <i>W. ryancraigii</i> sp. nov., and <i>W. secundiramea</i> sp. nov.) have complete rhizoidal cortication tightly covering axial cells of indeterminate axes below the apices, distinguishing them from the two local incompletely corticated congeners <i>W. argus</i> and <i>W. abscondita</i> sp. nov., the latter a morphologically cryptic sister species with <i>W. bicuspidata</i> from the Caribbean Sea. Only one of the new species, <i>W. ryancraigii</i>, has thus far been observed in the mesophotic zone off the Bermuda platform, and it is morphologically cryptic with the euphotic zone's <i>W. laxa</i>.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface currents shape protist community structure across the Indo-Pacific","authors":"Erin M. Borbee,&nbsp;Inna Ayu Puspa,&nbsp;Ester Restiana Endang Gelis,&nbsp;Fahkrizal Setiawan,&nbsp;Hawis Maduppa,&nbsp;Austin T. Humphries,&nbsp;Christopher E. Lane","doi":"10.1111/jpy.13465","DOIUrl":"10.1111/jpy.13465","url":null,"abstract":"<p>Biogeographic structure in marine protist communities is shaped by a combination of dispersal potential and environmental selection. High-throughput sequencing and global sampling efforts have helped better resolve the composition and functions of these communities in the world's oceans using both molecular and visual methods. However, molecular barcoding data are critically lacking across the Indo-Pacific, a region widely considered the epicenter of marine biodiversity. To fill this gap, we characterized protist communities in four sampling regions across Indonesia that represent the latitudinal, longitudinal, and human population gradients of the region: Lombok, Wakatobi, Misool, and Waigeo. We show high spatial structuring in marine protist communities across Indonesia, and biotic factors appear to play little role in driving this observed structure. Our results appear to be driven by abiotic factors linked to surface current patterns across the Indo-Pacific as a result of: (1) a choke point in circulation at the Indonesian Throughflow leading to low diatom diversity in Lombok, Wakatobi, and Misool; (2) an increase in nutrient availability at the edge of the Halmahera Eddy in Waigeo, leading to an increase in diatom diversity; and/or (3) seasonal variations in protist communities in line with shifts in velocity of the Indonesian Throughflow. Overall, our results highlight the importance of abiotic factors in shaping protist communities on broad geographic scales over biotic, top-down pressures, such as grazing from higher trophic levels.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diatom species responses along gradients of dissolved inorganic carbon, total phosphorus, and lake depth from lakes across Canada","authors":"Katherine Griffiths,&nbsp;Matthew P. Duda,&nbsp;Dermot Antoniades,&nbsp;John P. Smol,&nbsp;Irene Gregory-Eaves","doi":"10.1111/jpy.13464","DOIUrl":"10.1111/jpy.13464","url":null,"abstract":"<p>Diatoms are key components of freshwater ecosystems and are regularly used for paleolimnological reconstructions, in which defining species optima and tolerances is fundamental for interpreting assemblage shifts in a sediment record. Here, we examined responses of diatoms across three major environmental gradients—dissolved inorganic carbon (range: 0.1–230.5 mg · L⁻¹), total phosphorus (range: 3–326 μg · L⁻¹), and maximum lake depth (range: 0.9–55.0 m)—taken from 158 lakes from across Canada. The lakes were sampled as part of the LakePulse Network, which conducted a standardized sampling of lakes spanning 12 Canadian ecozones. Hierarchical logistic regression was used to model the species responses of 37 common taxa, and species optima and tolerances were calculated with weighted average modeling. The most common response detected was the symmetrical unimodal model, suggesting we likely captured the full environmental ranges for many species, although skewed unimodal responses were also common. Indicator species analyses identified taxa with high predictive values and fidelities to particular ecozones, with high-nutrient-adapted taxa such as <i>Stephanodiscus</i> spp. and <i>Cyclotella meneghiniana</i> characteristic of the agriculturally productive Prairie region. The Prairies stood out in the dataset as the region with the most unique flora from the local contribution to beta diversity analysis. Overall, the autecological data provided by our study will allow for improved interpretations of paleolimnological records and other biomonitoring efforts, addressing management concerns and contributing to a better understanding of our changing environment.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13464","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sinking rates, orientation, and behavior of pennate diatoms","authors":"M. Sourisseau,&nbsp;J. Font-Muñoz,&nbsp;S. Bellouche,&nbsp;O. Fauvarque,&nbsp;J. Rouxel,&nbsp;M. Tardivel,&nbsp;A. Sauvey","doi":"10.1111/jpy.13463","DOIUrl":"10.1111/jpy.13463","url":null,"abstract":"<p>Phytoplankton cells are now recognized as dynamic entities rather than as passive and isolated particles because they can actively modulate impacts of selection factors (nutrients, light, turbidity, and mixing) through a wide range of adaptations. Cell shape and/or chain length modulation is one of these processes but has predominantly been studied as an adaptation or an acclimatation to a specific growth limitation (light, nutrients, predation, etc.). In this study we have demonstrated that cell shape and size may have greater roles than previously known in phytoplankton ecology and species adaptation by permitting cell-to-cell signaling and more complex ecological processes that result from it. By exploring microscale biophysical interactions that lead to specific cell reorientation processes, we demonstrated that cell geometry not only modulates cell sinking rates but can also provide fast sensor responses to the cells' environment. Although gyrotaxis has been described in detail for motile phytoplankton cells, our findings illustrate that the reorientation process described here can occur even in non-motile cells within their natural environment. An additional consistent behavior was also recently described for a diatom species (<i>Pseudo-nitzschia delicatessima</i>), and with this study, we extend this observation to <i>Pseudo-nitzschia pungens</i> and <i>Pseudo-nitzschia fraudulenta</i>. Our observations emphasize the generality of this process, which adds a new level of complexity to our understanding of cellular interactions and their network of sensors.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jpy.13463","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}