Journal of Plant Research最新文献

{"title":"Regulatory responses to phosphorus deficiency in pecan (Carya illinoinensis): transcriptomic and microscopic approaches.","authors":"Siyuan Qian, Jianhang Xu, Xinyu Shen, Shuzhe Wang, Chenchen Zhuang, Caiyun Li, Zhengfu Yang, Kean-Jin Lim, Zhengjia Wang","doi":"10.1007/s10265-026-01744-x","DOIUrl":"10.1007/s10265-026-01744-x","url":null,"abstract":"<p><p>Phosphorus (P) deficiency in the soil significantly limits pecan growth due to its low availability and mobility. However, the molecular mechanisms underlying these responses to P deficiency are unclear. Therefore, this study aimed to describe adaptive strategies in pecan under varying P levels using microscopic and transcriptomic approaches to investigate pecans' adaptive mechanisms under three different P levels: normal phosphorus control (CK), low phosphorus (LP), and no-phosphorus (NP) deficiency. Compared with CK, we obtained 804 and 2,044 differentially expressed genes (DEGs) for LP and NP deficiency. The hierarchical clustering analysis categorized these DEGs into seven modules with distinct expression profiles. Functional enrichment analyses revealed that glycolysis and starch metabolism-related DEGs were consistently down-regulated, indicating a common response to LP and NP. Conversely, polysaccharide synthesis DEGs were considerably up-regulated under NP stress. The expression patterns of related DEGs were further validated using reverse transcription quantitative PCR (RT-qPCR). Our microscopic observations were consistent with these findings, showing increased root tip starch granules under P deficiency stress. Together, these findings suggest that pecan seedlings showed two coordinated responses to P deficiency: reduced expression of catabolic pathways and increased polysaccharide biosynthesis under NP, accompanied by higher root-tip starch granules and enhanced root architecture. This study elucidates the response of pecan to P deficiency and highlights potential targets for improving phosphorus use efficiency in woody oil crops.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"897-910"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HOMEODOMAIN GLABROUS 4 promotes DNA damage-induced stem cell death in Arabidopsis thaliana roots.","authors":"Toshiki Wada, Mihiro Nakajima, Naoki Takahashi","doi":"10.1007/s10265-026-01741-0","DOIUrl":"10.1007/s10265-026-01741-0","url":null,"abstract":"<p><p>Maintenance of genomic integrity is essential for sustained cell proliferation in plant meristems. In Arabidopsis thaliana, the DNA damage response is controlled by the transcription factor SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1), which regulates the expression of genes involved in DNA repair, cell cycle arrest, and stem cell death. However, the mechanisms underlying the correlation between DNA damage signaling and the regulation of stem cell death remain incompletely understood. In this study, we identified HOMEODOMAIN GLABROUS 4 (HDG4), a member of the homeodomain leucine zipper class IV transcription factor family, as a factor promoting DNA damage-induced stem cell death in the root meristem. Reporter analyses revealed that HDG4 expression is strongly induced in the root meristem following DNA damage. Moreover, genetic analysis revealed that DNA damage-induced stem cell death was significantly suppressed in the hdg4 mutant. The DNA damage-associated repression of auxin efflux carriers, PIN-FORMED (PIN), was partially alleviated in the hdg4 mutant. Consistent with this observation, the reduction in the auxin response in the root tip after DNA damage was attenuated in the hdg4 mutant. These results indicate that HDG4 contributes to the suppression of auxin transport toward the root tip during DNA damage response, and reveal a transcriptional mechanism linking genome integrity surveillance with hormonal regulation of stem cell death.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"885-895"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2026 Awards in the Journal of Plant Research.","authors":"Noriko Inada","doi":"10.1007/s10265-026-01752-x","DOIUrl":"https://doi.org/10.1007/s10265-026-01752-x","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Intricate intracellular kinase network regulates the Spodoptera litura-derived elicitor response signaling in Arabidopsis.","authors":"Yoshitake Desaki, Tasuku Kato, Keiichirou Nemoto, Akira Nozawa, Takuya Uemura, Naoya Ninomiya, Tatsuya Sawasaki, Gen-Ichiro Arimura","doi":"10.1007/s10265-026-01736-x","DOIUrl":"https://doi.org/10.1007/s10265-026-01736-x","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148818603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbarium specimens in the age of artificial intelligence: from herbarium image identification to Integrative Taxonomic AI.","authors":"Atsuko Takano, Hyeonji Moon, Masato Shirai, Shuichiro Tagane, Diego Tavares Vasques, Chung-Kun Lee, Amjad Khan, Jeongwoo Seo, Sujeong Han, Chanho Park, Jaesung Lee, Takashi Akihiro, Sangtae Kim","doi":"10.1007/s10265-026-01743-y","DOIUrl":"https://doi.org/10.1007/s10265-026-01743-y","url":null,"abstract":"<p><p>Herbarium specimens are physical, verifiable records that form the basis of taxonomic knowledge and biodiversity research. Their large-scale digitization has produced extensive collections of high-resolution images and associated specimen metadata, creating conditions in which artificial intelligence (AI) can play an important role in plant taxonomy, collection management, and ecological research. Early AI applications have primarily focused on automated species identification based on individual specimen images. Although increasingly accurate, such approaches remain limited by their emphasis on single-specimen label prediction and by treating identification outputs as final analytical decisions. Recent methodological advances-including segmentation-based preprocessing, automated trait extraction, structured extraction of label data, detection of potentially misidentified specimens, and multimodal integration of visual, textual, and genetic information-extend AI applications beyond species identification toward broader analytical frameworks, encompassing taxonomic interpretation as well as ecological and biodiversity research. In these approaches, specimens are placed within a shared analytical space, and identification results are used to support comparisons across multiple specimens rather than being treated as final decisions for single individuals. This multi-specimen perspective enables quantitative examination of species boundaries, morphological variation, data inconsistencies, and taxonomic stability within curated collections. In this context, AI serves not as an ultimate decision-maker but as a decision-support tool embedded in expert-guided workflows and biodiversity knowledge infrastructures. These developments can be summarized as Integrative Taxonomic AI, an approach that employs learned morphospaces to interpret and refine taxonomic categories by integrating multimodal evidence and curated specimen data under expert guidance.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148818516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat susceptibility evaluation among 100 mango (Mangifera indica) genotypes: a comparative study on leaf cell membrane stability at high temperatures using electrolyte leakage.","authors":"Hiroshi Matsuda, Sayo Shimizu, Hiroo Takaragawa, Hirokazu Higuchi","doi":"10.1007/s10265-026-01735-y","DOIUrl":"https://doi.org/10.1007/s10265-026-01735-y","url":null,"abstract":"<p><p>High-temperature stress induced by global warming can hinder the production of commercial cultivars in mangoes (Mangifera indica L.). Selecting cultivars with high-temperature tolerance is essential. We evaluated damage to leaf cell membranes as an indicator of high-temperature tolerance in mangoes. An evaluation method using leaf-electrolyte leakage was established by comparing the temperature responses of several genotypes, and then applied to 100 genotypes, including major cultivars worldwide. Genotypic differences in high-temperature tolerance were verified based on the climate of selection and production areas. Logistic regression analysis was performed on datasets from two different years for each genotype, with temperature (40-55 °C) as the explanatory variable, and the calculated Injury Index as the target variable. The obtained regression equations were highly accurate (R² > 0.9; RMSE < 10) for each genotype and year. Furthermore, a strong positive correlation (Spearman's rank correlation coefficient > 0.8) was obtained between the pair of temperature conditions and the maximum curvature on the regression curve (C_max) for two different years for each genotype, confirming the validity of the evaluation method. Up to ≈ 6 °C genotypic difference was observed in the mean C_max values. Cultivars from warm and humid Southeast Asia tended to have a higher cell membrane tolerance than those from dry India. Florida cultivars, mostly pedigreed from Southeast Asia and India, were widely allocated from high to low. We ranked 100 mango genotypes based on the high-temperature tolerance of leaf cell membranes, demonstrating reasonable consistency with the climatic conditions of their selection and production areas.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148818543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of the HDAC gene family in Pyrus betulifolia and functional validation of PcHDAC5 and PcHDT1.2 in Pyrus calleryana seed dormancy release.","authors":"Xiao-Xiong Kong, Ze-Kun Liu, Tao Chen, Jing Zhang, Xiao Liu, Zhi-Ping Zhang, Chun-Lei Wang","doi":"10.1007/s10265-026-01749-6","DOIUrl":"https://doi.org/10.1007/s10265-026-01749-6","url":null,"abstract":"<p><p>Histone deacetylation, catalyzed by histone deacetylases (HDACs), is a core epigenetic regulatory mechanism in plants. However, no systematic characterization of the HDAC gene family has been reported in pear (Pyrus spp.), a globally important temperate fruit crop. In this study, we performed genome-wide identification and functional characterization of HDAC genes in pear, with a specific focus on their regulatory roles in seed dormancy release. We identified 22 PbHDAC genes from the genome of Pyrus betulifolia Bunge, which were classified into three conserved subfamilies. Evolutionary analysis confirmed that all PbHDAC genes have undergone strong purifying selection. Transcriptome profiling of Pyrus calleryana Decne seeds identified seven PcHDAC genes differentially expressed during seed dormancy release, and quantitative real-time PCR (qRT-PCR) verified that only PcHDAC5 and PcHDT1.2 showed consistent significant differential expression throughout this process. Using an optimized antisense oligonucleotide (as-ODN)-mediated silencing system tailored for hard-coated pear seeds, we demonstrated that PcHDT1.2 and PcHDAC5 act as negative and positive regulators of seed dormancy release, respectively. Further experiments revealed that knockdown of these two genes altered the contents of abscisic acid (ABA) and indole-3-acetic acid (IAA). Collectively, this study not only fills the long-standing gap in systematic HDAC family research in pear, but also provides a robust technical workflow for functional gene validation in hard-coated seeds, with important implications for pear rootstock breeding and seed germination regulation.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive honeybees exacerbate the low reproductive success of a bat-pollinated tree.","authors":"Víctor Rosas-Guerrero, Rodrigo Lucas-García, Lorena Alemán-Figueroa, Mariana Ceballos-Torres","doi":"10.1007/s10265-026-01746-9","DOIUrl":"https://doi.org/10.1007/s10265-026-01746-9","url":null,"abstract":"<p><p>Some bat species play a crucial role as the main effective pollinators of many flowering plants. Nevertheless, other floral visitors may act as antagonists, affecting the reproductive success of these plants. In this study, we investigated some potential causes of the extremely low production of fruits of Crescentia alata, a neotropical tree supposedly pollinated by bats that is heavily visited by the introduced honeybee Apis mellifera in Guerrero, southern Mexico. Our results indicate that C. alata had an extremely low natural fruit set (0.2%) and total pollen limitation, probably due to multiple intrinsic factors, including a complete self-incompatible system, low herkogamy, and incomplete dichogamy; as well as extrinsic factors such as the high pollen removal associated with the numerous visits of the invasive honeybees before the arrival of bats, which were the most effective pollinators of this plant. Nectarivorous bat conservation through forest restoration, as well as a decrease of managed beehives and the plantation of genetically diverse individuals, are proposed as strategies to minimize the low reproduction of this neotropical tree of great importance to humans and to the maintenance of tropical dry forests.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Knockout of a single aquaporin, OsPIP2;4, decreases root water permeability in rice.","authors":"Aya Onishi, Tomoaki Horie, Ryo Ishitsuka, Shizuka Sasano, Rie Horie, Yunosuke Mito, Shigeko Utsugi, Junko Ishikawa, Majid Mahdieh, Maki Katsuhara","doi":"10.1007/s10265-026-01713-4","DOIUrl":"10.1007/s10265-026-01713-4","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"681"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148005627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATP-dependent binding cassette transporter ClABCI6 promotes the expression of citrus yellow vein clearing virus via inhibiting salicylic acid pathway.","authors":"Yaqin Song, Wen Ge, Cuiyun Lei, Yijie Li, Yang Han, Jiedan Xiao, Xinghua Qiao, Shixue Hu, Yan Zhou, Binghai Lou","doi":"10.1007/s10265-026-01719-y","DOIUrl":"10.1007/s10265-026-01719-y","url":null,"abstract":"<p><p>ATP-dependent binding cassette (ABC) transporters constitute a class of proteins that utilize ATP hydrolysis to actively transport diverse substrates across cellular membranes and play crucial roles in various physiological processes in plants. However, the specific function of ABC transporters in plant antiviral defense remains poorly characterized. In this study, the homologous ABCI6 proteins originating from Citrus L. spp. showed high evolutionary conservation (higher than 75.82%). The expression of ClABCI6, the ABCI6 homologous gene in Eureka lemon (Citrus limon (L.) Osbeck), was significantly upregulated following infection with citrus yellow vein clearing virus (CYVCV). Further study showed that ClABCI6 localized specifically to the chloroplast and physically interacted with the RNA-dependent RNA polymerase domain (He-RdRp) of CYVCV using yeasts two-hybrid assays. In addition, ClABCI6 promoted the expression of He-RdRp and CYVCV, while concurrently suppressing the expression of salicylic acid (SA) pathway genes in lemon, thus facilitating viral proliferation. This study provides novel insights into the evolution and molecular function of ClABCI6, and reveals its response patterns to CYVCV-infection.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"655-665"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147973243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}