Journal of Plant Research最新文献_第4页

Detection of reproductive interference between closely related Salvia species with small-scale separated distributions by multifaceted pollination and molecular analyses. 通过多方面的授粉和分子分析，检测小范围内分布分离的近缘丹参物种之间的生殖干扰。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-30 DOI: 10.1007/s10265-024-01577-6

Sachiko Nishida, Atsuko Takano, Yoshihisa Suyama, Satoshi Kakishima

{"title":"Detection of reproductive interference between closely related Salvia species with small-scale separated distributions by multifaceted pollination and molecular analyses.","authors":"Sachiko Nishida, Atsuko Takano, Yoshihisa Suyama, Satoshi Kakishima","doi":"10.1007/s10265-024-01577-6","DOIUrl":"https://doi.org/10.1007/s10265-024-01577-6","url":null,"abstract":"Reproductive interference, an interspecific interaction in reproductive process that exerts an adverse effect, has gained attention as a contributing factor in promoting exclusive distributions between closely related species. However, detailed studies on the possibility of reproductive interference between native plants are still lacking, presumably because strong reproductive interference can rapidly realize exclusive distributions, leaving the two species apparently independent. Salvia japonica and S. lutescens are found in separate localities at a small scale, although their distributions overlap at a large scale. We investigated the possibility of reproductive interference between them through field surveys, hand-pollination experiments, evaluation of hybrid fertility, cpDNA and nrDNA genotyping, and genome-wide DNA analysis. The field survey results did not reveal apparent negative interaction in competition for pollinator services. Mixed pollination with conspecific pollen and counterpart pollen reduced seed set in S. japonica, and hybrid progeny produced by mixed pollination were less than 20% as fertile compared to the pure species. The DNA genotyping results suggested the possibility of hybridization where their distributions overlap, and the genome-wide DNA analysis results showed clear genetic differentiation between the two species as well as the existence of hybrids. These results suggest that bi-directional reproductive interference between S. japonica and S. lutescens may have led to their present separated distributions at a small scale.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of seed germination, seedling growth and physiological characteristics to alkali stress in halophyte Suaeda liaotungensis. 盐生植物 Suaeda liaotungensis 的种子萌发、幼苗生长和生理特征对碱胁迫的响应。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-28 DOI: 10.1007/s10265-024-01568-7

Jieqiong Song, Lantong Zhao, Yiming Ma, Xiaoqi Cao, Ruixuan An, Jingying Zhao, Haoran Ding, Hongfei Wang, Changping Li, Qiuli Li

{"title":"Response of seed germination, seedling growth and physiological characteristics to alkali stress in halophyte Suaeda liaotungensis.","authors":"Jieqiong Song, Lantong Zhao, Yiming Ma, Xiaoqi Cao, Ruixuan An, Jingying Zhao, Haoran Ding, Hongfei Wang, Changping Li, Qiuli Li","doi":"10.1007/s10265-024-01568-7","DOIUrl":"https://doi.org/10.1007/s10265-024-01568-7","url":null,"abstract":"Soil salinization has been considered as a major environmental threat to plant growth. Different types of salt in saline soil have different effects on germination and seedling growth. Effect of NaCl on germination and seedling establishment in Suaeda liaotungensis have been reported, but its response to alkali stress remains unclear. Our results showed that brown seeds had higher germination rate, however, black seeds had higher germination recovery percentage under alkali stress. Na2CO3 had stronger inhibitory effect on germination and seedling growth than NaHCO3. As the concentration of alkali stress increased, the ROS level of brown seeds gradually ascended, while that of black seeds decreased first and then ascended. MDA content of dimorphic seeds significantly increased under alkali stress. The trend of SOD, POD and CAT activity between dimorphic seeds was similar under the same type of alkali stress. Alkali stress enhanced proline content of dimorphic seeds, and dimorphic seeds in NaHCO3 solution had higher proline content than Na2CO3 solution. Moreover, radicle and shoot tolerance indexes of seedlings in NaHCO3 solution were significantly higher than that of Na2CO3 solution. Under strong alkali stress, seedlings in NaHCO3 solution had significantly lower ROS level and MDA content as well as higher antioxidant enzyme activity than Na2CO3 solution. This study comprehensively compared the morphological and physiological characteristics in germination and seedlings to better reveal the saline-alkali tolerance mechanisms in S. liaotungensis.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142093552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic positions of Thai members of Gymnema, Gymnemopsis and Sarcolobus (Apocynaceae, Asclepiadoideae, Marsdenieae), and two new Sarcolobus species uncovered by morpho-molecular evidence. Gymnema、Gymnemopsis 和 Sarcolobus（Apocynaceae，Asclepiadoideae，Marsdenieae）泰国成员的系统发育位置，以及通过形态分子证据发现的两个 Sarcolobus 新种。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-27 DOI: 10.1007/s10265-024-01562-z

Manit Kidyoo, Aroonrat Kidyoo, Doyle McKey

{"title":"Phylogenetic positions of Thai members of Gymnema, Gymnemopsis and Sarcolobus (Apocynaceae, Asclepiadoideae, Marsdenieae), and two new Sarcolobus species uncovered by morpho-molecular evidence.","authors":"Manit Kidyoo, Aroonrat Kidyoo, Doyle McKey","doi":"10.1007/s10265-024-01562-z","DOIUrl":"10.1007/s10265-024-01562-z","url":null,"abstract":"The present study assesses the phylogenetic position of certain Thai members of Gymnema, Gymnemopsis, and Sarcolobus in relation to other known Marsdenieae species. Fifteen accessions newly sequenced from Thailand were added to the dataset of the homologous sequences of 125 accessions of Marsdenieae downloaded from GenBank. In our molecular phylogeny, almost all the delimited major clades and their relationships are largely congruent with those revealed in previous studies. The monophyly of Gymnema (including the former Jasminanthes species) and that of Sarcolobus, as presently circumscribed, are confirmed. The new accessions of these two genera from Thailand are well grouped with the members of their respective genera. Our analyses provide the first molecular evidence for recognition of Gymnemopsis, a small Asian genus that has never been included in the previous phylogenetic studies, as a distinct genus. All elements of Gymnemopsis are retrieved as a well-supported monophyletic group that is strongly supported as sister to Lygisma, another small Asian genus that most closely resembles it in growth habit, color of latex, indumentum on plant parts, corona structure and follicle traits. Combined molecular phylogenetic, morphological and ecological data also support recognition of two new Sarcolobus species from Thailand, Sarcolobus busbanianus sp. nov. and S. flavus sp. nov. Similarities and differences between these new species and their close relative, S. carinatus, are discussed. In addition, this study also reveals the first record for Thailand of Gymnema lacei. Keys to the species of Gymnemopsis (for all members of the genus), Gymnema and Sarcolobus (for Thai members of these genera) are provided.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A resilient mutualistic interaction between cucumber mosaic virus and its natural host to adapt to an excess zinc environment and drought stress. 黄瓜花叶病毒与其自然宿主之间为适应过量锌环境和干旱胁迫而产生的一种顽强的互利互动关系。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-27 DOI: 10.1007/s10265-024-01573-w

Midori Tabara, Shimpei Uraguchi, Masako Kiyono, Izumi Watanabe, Atsushi Takeda, Hideki Takahashi, Toshiyuki Fukuhara

{"title":"A resilient mutualistic interaction between cucumber mosaic virus and its natural host to adapt to an excess zinc environment and drought stress.","authors":"Midori Tabara, Shimpei Uraguchi, Masako Kiyono, Izumi Watanabe, Atsushi Takeda, Hideki Takahashi, Toshiyuki Fukuhara","doi":"10.1007/s10265-024-01573-w","DOIUrl":"https://doi.org/10.1007/s10265-024-01573-w","url":null,"abstract":"A perennial pseudometallophyte Arabidopsis halleri is frequently infected with cucumber mosaic virus (CMV) in its natural habitat. The purpose of this study was to characterize the effect of CMV infection on the environmental adaptation of its natural host A. halleri. The CMV(Ho) strain isolated from A. halleri was inoculated into clonal virus-free A. halleri plants, and a unique plant-virus system consisting of CMV(Ho) and its natural wild plant host was established. In a control environment with ambient zinc supplementation, CMV(Ho) infection retarded growth in the above-ground part of host plants but conferred strong drought tolerance. On the other hand, in an excess zinc environment, simulating a natural edaphic environment of A halleri, host plants hyperaccumulated zinc and CMV(Ho) infection did not cause any symptoms to host plants while conferring mild drought tolerance. We also demonstrated in Nicotiana benthamiana as another host that similar effects were induced by the combination of excess zinc and CMV(Ho) infection. Transcriptomic analysis indicated that the host plant recognized CMV(Ho) as a mutualistic symbiont rather than a parasitic pathogen. These results suggest a resilient mutualistic interaction between CMV(Ho) and its natural host A. halleri in its natural habitat.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Network analyses show horizontal and vertical distribution of vascular epiphytes on their hosts in a fragment of cloud forest in Central Mexico. 网络分析显示了墨西哥中部云雾森林片段中维管束附生植物在其寄主上的水平和垂直分布。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-24 DOI: 10.1007/s10265-024-01569-6

Elizabeth Victoriano-Romero, Dulce María Figueroa-Castro, Jonas Morales-Linares

{"title":"Network analyses show horizontal and vertical distribution of vascular epiphytes on their hosts in a fragment of cloud forest in Central Mexico.","authors":"Elizabeth Victoriano-Romero, Dulce María Figueroa-Castro, Jonas Morales-Linares","doi":"10.1007/s10265-024-01569-6","DOIUrl":"https://doi.org/10.1007/s10265-024-01569-6","url":null,"abstract":"Cloud forests figure as one of the most diverse ecosystems, accounting not only for a high number of plant species but also with a great variety of interactions among them. A common interaction in these forests is the one between vascular epiphytes and their hosts. However, few studies have used the network approach to analyze them. Here, we analyze the horizontal and vertical structure of the vascular epiphyte - host network in a cloud forest in central Mexico. We quantified the number of epiphyte stands on each host both total and per-stratum. Complete network, group, and species metrics were estimated at both levels of analysis. The host - epiphyte networks had relatively low network size but were highly connected; moderately nested, with low specialization, and modularity; but higher vulnerability than generality, and high niche overlap. The community was composed by a high number of generalist species. To our knowledge this is the first study in which network analyses are conducted with standardized data and including all host and epiphyte species in the community. The analyses suggest that the networks are robust, and that functional redundancy might be probable, two advantageous characteristics in a very fragmented and threatened cloud forest.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

East Asian forest-steppe outpost in the Khanka Lowland (Russia) and its conservation. 俄罗斯汉卡低地的东亚森林草原前哨及其保护。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-24 DOI: 10.1007/s10265-024-01570-z

Elena A Marchuk, Anastasiya K Kvitchenko, Lyubov A Kameneva, Aleksandra A Yuferova, Dmitry E Kislov

{"title":"East Asian forest-steppe outpost in the Khanka Lowland (Russia) and its conservation.","authors":"Elena A Marchuk, Anastasiya K Kvitchenko, Lyubov A Kameneva, Aleksandra A Yuferova, Dmitry E Kislov","doi":"10.1007/s10265-024-01570-z","DOIUrl":"https://doi.org/10.1007/s10265-024-01570-z","url":null,"abstract":"The Khanka Lowland forest-steppe is the most eastern outpost of the Eurasian steppe biome. It includes unique grassland plant communities with rare steppe species. These coenosis have changed under the influence of anthropogenic activity, especially during the last 100 years and included both typical steppe species and nemoral mesophytic species. To distinguish these ecological groups of plants the random forest method with three datasets of environmental variables was applied. Specifically, a model of classification with the most important bioindices to predict a mesophytic ecological group of plants with a sensitivity greater than 80% was constructed. The data demonstrated the presence of steppe species that arrived at different times in the Primorye Territory. Most of these species are associated with the Mongolian-Daurian relict steppe complex and habit in the Khanka Lowland. Other species occur only in mountains in Primorye Territory and do not persist in the Khanka Lowland. These findings emphasize the presence of relict steppe communities with a complex of true steppe species in the Khanka Lowland. Steppe communities exhibit features of anthropogenic influence definitely through the long land use period but are not anthropogenic in origin. The most steppe species are located at the eastern border of distribution in the Khanka Lowlands and are valuable in terms of conservation and sources of information about steppe species origin and the emergence of the steppe biome as a whole.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light promotes asexual reproduction and mediates transcriptomic changes in Pediastrum duplex. 光能促进无性繁殖，并介导双壳贝母的转录组变化。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-20 DOI: 10.1007/s10265-024-01567-8

Akari Masaki, Narumi Miyamoto, Sridharan Harshavardhini, Noriko Nagata, Yuki Tsuchikane, Hiroyuki Sekimoto, Yutaka Kodama, Tomohiro Suzuki, Tomoko Shinomura

引用次数: 0

Chemotaxonomy compared to morphological and anatomical taxonomy of five Hibiscus species. 将化学分类法与五个芙蓉品种的形态和解剖分类法进行比较。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-15 DOI: 10.1007/s10265-024-01566-9

Hala M E Abdelfattah, Hussein A Hussein, Samir S Teleb, Marwa M El-Demerdash, Nelly M George

{"title":"Chemotaxonomy compared to morphological and anatomical taxonomy of five Hibiscus species.","authors":"Hala M E Abdelfattah, Hussein A Hussein, Samir S Teleb, Marwa M El-Demerdash, Nelly M George","doi":"10.1007/s10265-024-01566-9","DOIUrl":"https://doi.org/10.1007/s10265-024-01566-9","url":null,"abstract":"Hibiscus is a charismatic genus of the Malvaceae family that is noted for its diversity, lacking identifiable characteristics for distinguishing its various species. Therefore, there is an urgent need to develop authentication methods for genus delimitation and species delineation. The present study aims to discern the taxonomic relationships between the well-known, globally familiar, and economically important five Hibiscus species, namely: H. × rosa-sinensis, H. sabdariffa, H. schizopetalus, H. syriacus and H. tiliaceus based on traditional morphological and anatomical characteristics compared to the contemporary chemotaxonomy. In this context, the leaf-based methanolic extracts of the studied species were analyzed by Gas Chromatography-Mass Spectrometer (GC-MS) to estimate their secondary metabolites similarity. In addition, selected qualitative morphological and anatomical traits including leaf venation patterns, epidermal micromorphology, stomata types and trichomes diversity, petiole serial sectioning (outline, adaxial groove features, vasculature traces arrangement), and midrib characteristics of the studied species were investigated. The results of both chemotaxonomy and traditional taxonomy exhibited a remarkable agreement in the delineation of the five studied species. Specifically, the chemotaxonomy-based dendrogram separates the studied species into two main clusters with the H. sabdariffa as an outlier species in a single cluster and the remaining four species as another cluster with variant distances in its similarity indices. Similarly, the traditional morphological and anatomical characteristics revealed distinct traits for H. sabdariffa compared to the remaining four species. The findings of this study highlight the significance of integrating the structural features with phytochemicals profiling as a potential approach that could be harnessed for the delineation of the taxonomically challenging Hibiscus genus.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotype identification and genome-wide association study of ear-internode vascular bundles in maize (Zea mays). 玉米（Zea mays）穗间维管束的表型鉴定和全基因组关联研究。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-07 DOI: 10.1007/s10265-024-01565-w

Huan Zhao, Ying Zhang, Xianju Lu, Yanxin Zhao, Chuanyu Wang, Weiliang Wen, Minxiao Duan, Shuaihao Zhao, Jinglu Wang, Xinyu Guo

{"title":"Phenotype identification and genome-wide association study of ear-internode vascular bundles in maize (Zea mays).","authors":"Huan Zhao, Ying Zhang, Xianju Lu, Yanxin Zhao, Chuanyu Wang, Weiliang Wen, Minxiao Duan, Shuaihao Zhao, Jinglu Wang, Xinyu Guo","doi":"10.1007/s10265-024-01565-w","DOIUrl":"https://doi.org/10.1007/s10265-024-01565-w","url":null,"abstract":"The vascular bundle in the ear-internode of maize is a key conduit for transporting photosynthetic materials between \"source\" and \"sink\", making it critically important to examine its micro-phenotypes and genetic architecture to identify advantageous characteristics and cultivate high-yielding and high-quality varieties. Unfortunately, the limited observation methods and scope of study precludes any comprehensive and systematic investigations into the microscopic phenotypes and genetic mechanisms of vascular bundle in maize ear-internode. In this study, 47 phenotypic traits were extracted in 495 maize inbred lines using micro computed tomography (Micro-CT) scanning technology and a deep learning-based phenotype acquisition method for stem vascular bundle, which included stem slice-related, epidermis zone-related, periphery zone-related, inner zone-related and vascular bundles-related traits. Phenotypic analysis indicated that there was extensive phenotypic variation of vascular bundle traits in ear-internode, especially that in the inner zone. Of these, 30 phenotypic traits with heritability greater than 0.70 were conducted for GWAS, and a total of 4,225 significant SNPs and 416 candidate genes with detailed functional annotations were identified. Furthermore, 20 genes were highly expressed in stem-related tissues, especially in maize internodes. Functional analysis of candidate genes indicated that the pathways obtained for candidate genes of different trait groups were distinct, mainly involved in vitamin synthesis and metabolism, transport of substances, carbohydrate derivative catabolic process, protein transport and localization, and anatomical structure development. The results of this study will help to further understand the phenotypic traits of stem vascular bundles and provide a reference for revealing the genetic mechanism of maize ear-internode vascular bundles.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SENSITIVE TO FREEZING2 is crucial for growth of Marchantia polymorpha under acidic conditions. SENSITIVE TO FREEZING2 对多孔菌在酸性条件下的生长至关重要。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-08-04 DOI: 10.1007/s10265-024-01564-x

Shinsuke Shimizu, Koichi Hori, Kimitsune Ishizaki, Hiroyuki Ohta, Mie Shimojima

{"title":"SENSITIVE TO FREEZING2 is crucial for growth of Marchantia polymorpha under acidic conditions.","authors":"Shinsuke Shimizu, Koichi Hori, Kimitsune Ishizaki, Hiroyuki Ohta, Mie Shimojima","doi":"10.1007/s10265-024-01564-x","DOIUrl":"https://doi.org/10.1007/s10265-024-01564-x","url":null,"abstract":"Land plants have evolved many systems to adapt to a wide range of environmental stresses. In seed plants, oligogalactolipid synthesis is involved in tolerance to freezing and dehydration, but it has not been analyzed in non-vascular plants. Here we analyzed trigalactosyldiacylglycerol (TGDG) synthesis in Marchantia polymorpha. TGDG is synthesized by galactolipid: galactolipid galactosyltransferase [GGGT; SENSITIVE TO FREEZING2 (SFR2) in Arabidopsis]. We analyzed the subcellular localization and GGGT activity of two M. polymorpha SFR2 homologs (MpGGGT1 and MpGGGT2, each as a GFP-fusion protein) using a transient expression system in Nicotiana benthamiana leaves and found that MpGGGT1-GFP localized in the chloroplast envelope membrane. We produced mutants Mpgggt1 and Mpgggt2 and found that TGDG did not accumulate in Mpgggt1 upon treatment of the thallus with acetic acid. Moreover, growth of Mpgggt1 mutants was impaired by acetic acid treatment. Microscopy revealed that the acetic acid treatment of M. polymorpha plants damaged intracellular membranes. The fact that the effect was similar for wild-type and Mpgggt1 plants suggested that MpGGGT has a role in recovery from damage. These results indicate that MpGGGT plays a crucial role in M. polymorpha growth under conditions of acid stress, which may have been encountered during the ancient terrestrial colonization of plants.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0