Journal of Plant Research最新文献_第4页

Enzymatic and quantitative properties of Rubisco in some conifers and lycopods. 一些针叶树和石松中Rubisco的酶学和定量性质。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-14 DOI: 10.1007/s10265-024-01606-4

Sakiko Sugawara, Kana Ito, Shin-Ichi Miyazawa, Amane Makino, Yuji Suzuki

{"title":"Enzymatic and quantitative properties of Rubisco in some conifers and lycopods.","authors":"Sakiko Sugawara, Kana Ito, Shin-Ichi Miyazawa, Amane Makino, Yuji Suzuki","doi":"10.1007/s10265-024-01606-4","DOIUrl":"https://doi.org/10.1007/s10265-024-01606-4","url":null,"abstract":"Information on the kinetic properties of Rubisco, a key enzyme for photosynthesis, is scarce in land plants that emerged early during the evolutionary process. This study examined the carboxylase activity and abundance of Rubisco in five conifers, two lycopods, and three control C3 crops. The turnover rates of Rubisco carboxylation (kcatc) under saturated-CO2 conditions in conifers and lycopods were comparable to those in the control C3 crops. Rubisco carboxylase activity under CO2-unsaturated conditions (vcu) was also measured using reaction mixtures saturated with a N2 gas containing CO2 and O2 at present atmospheric levels to predict the Rubisco CO2 affinity from the percentage of vcu in kcatc. The predicted CO2 affinity in conifers and lycopods tended to be lower than that in the control C3 crops. When the control C3 crops and two previously examined C4 crops were analyzed together, the kcatc of Rubisco with a low CO2 affinity tended to be high. N allocation to Rubisco with a low kcatc tended to be high in these plants. In conifers and lycopods, the kcatc was lower than that expected on the basis of predicted Rubisco CO2 affinity, unlike in the control crops. N allocation to Rubisco also tended to be lower than that expected on the basis of kcatc. These results indicate that Rubisco in the examined conifers and lycopods is not superior in terms of both kcatc and CO2 affinity and that the abundance of Rubisco is not necessarily closely related to its kinetic properties. The reason for these phenomena is discussed in terms of the molecular evolution of Rubisco.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATML1 and PDF2 regulate cuticle formation and protect the plant body from environmental stresses in Arabidopsis thaliana seedlings. ATML1和PDF2调控拟南芥幼苗角质层形成，保护植物体免受环境胁迫。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-10 DOI: 10.1007/s10265-024-01604-6

Kenji Nagata, Ichiro Maekawa, Taku Takahashi, Mitsutomo Abe

{"title":"ATML1 and PDF2 regulate cuticle formation and protect the plant body from environmental stresses in Arabidopsis thaliana seedlings.","authors":"Kenji Nagata, Ichiro Maekawa, Taku Takahashi, Mitsutomo Abe","doi":"10.1007/s10265-024-01604-6","DOIUrl":"https://doi.org/10.1007/s10265-024-01604-6","url":null,"abstract":"A sessile lifestyle compels plants to endure an array of environmental stressors in the location where they grow. To cope with environmental stresses, plants have developed specialized cell wall structures called cuticles at the interface between the plant and the environment. In Arabidopsis thaliana seedlings, cuticles cover and protect aerial organs and young roots. However, the precise assembly of the molecular machinery required for cuticle formation on the surface of distinct organs that exhibit entirely different functions and developmental contexts remains unknown. Here, we demonstrate that a paralogous gene pair, ARABIDOPSIS THALIANA MERISTEM LAYER1 (ATML1) and PROTODERMAL FACTOR2 (PDF2), regulates precise cuticle formation in Arabidopsis thaliana seedlings. We found that the expression of ATML1 and PDF2 spatially overlapped with cuticle deposition in Arabidopsis thaliana seedlings. Furthermore, the loss of ATML1 and PDF2 activity resulted in a significant downregulation of the expression of genes required for cuticle formation and compromised cuticle formation in different organs. Seedlings with impaired activities of ATML1 and PDF2 exhibited higher susceptibility to environmental stress. In particular, PDF2 plays a predominant role in tolerance to environmental stress rather than ATML1 in the roots. Collectively, our study provides new insights into the regulatory mechanisms of cuticle formation and the developmental strategies plants use to protect their bodies from environmental stresses.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormone functions in adventitious root formation during cutting propagation of woody plants. 木本植物扦插繁殖过程中激素在不定根形成中的作用。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-10 DOI: 10.1007/s10265-024-01602-8

Shujing Liu, Xinghui Li, Lin Xu, Guifang Zhang

引用次数: 0

Cell wall thickness spectrum of photosynthetic cells in herbaceous C₃, C₄, and crassulacean acid metabolism plants. 草本C3、C4和天竺葵酸代谢植物光合细胞的细胞壁厚度谱。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-10 DOI: 10.1007/s10265-024-01603-7

Osamu Ueno

{"title":"Cell wall thickness spectrum of photosynthetic cells in herbaceous C3, C4, and crassulacean acid metabolism plants.","authors":"Osamu Ueno","doi":"10.1007/s10265-024-01603-7","DOIUrl":"https://doi.org/10.1007/s10265-024-01603-7","url":null,"abstract":"Higher plants are divided into three major photosynthetic groups known as C3, C4, and crassulacean acid metabolism (CAM) plants. It is considered that cell wall thickness (TCW) affects diffusion and leakiness of CO2 within leaves, but it is unclear whether TCW of photosynthetic cells differs among these groups. This study investigated TCW of photosynthetic cells in herbaceous C3, C4, and CAM species under an electron microscope. Among 75 species of monocots and eudicots grown in a growth chamber in the same environment, the TCW of mesophyll cells (MCs) was much higher in CAM species than in C3 and C4 species. However, when TCW was compared between C3 and C4 species of grasses and eudicots, TCW of MCs tended to be lower in C4 species than in C3 species; the opposite trend was observed for TCW of bundle sheath cells (BSCs). TCW of MCs and BSCs almost did not differ among the C4 decarboxylation types (NADP-ME, NAD-ME, and PCK). In plants grown outdoors (51 species), similar trends of TCW were also found among photosynthetic groups, but their TCW was generally higher than that of growth-chamber plants. This study provides the TCW spectrum of photosynthetic cells in herbaceous C3, C4, and CAM species. The results obtained would be valuable for our understanding of the diffusion and leakage of CO2 in the leaves of different photosynthetic groups.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of reproductive interference between closely related Salvia species with small-scale separated distributions by multifaceted pollination and molecular analyses. 通过多方面的授粉和分子分析，检测小范围内分布分离的近缘丹参物种之间的生殖干扰。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-30 DOI: 10.1007/s10265-024-01577-6

Sachiko Nishida, Atsuko Takano, Yoshihisa Suyama, Satoshi Kakishima

{"title":"Detection of reproductive interference between closely related Salvia species with small-scale separated distributions by multifaceted pollination and molecular analyses.","authors":"Sachiko Nishida, Atsuko Takano, Yoshihisa Suyama, Satoshi Kakishima","doi":"10.1007/s10265-024-01577-6","DOIUrl":"10.1007/s10265-024-01577-6","url":null,"abstract":"Reproductive interference, an interspecific interaction in reproductive process that exerts an adverse effect, has gained attention as a contributing factor in promoting exclusive distributions between closely related species. However, detailed studies on the possibility of reproductive interference between native plants are still lacking, presumably because strong reproductive interference can rapidly realize exclusive distributions, leaving the two species apparently independent. Salvia japonica and S. lutescens are found in separate localities at a small scale, although their distributions overlap at a large scale. We investigated the possibility of reproductive interference between them through field surveys, hand-pollination experiments, evaluation of hybrid fertility, cpDNA and nrDNA genotyping, and genome-wide DNA analysis. The field survey results did not reveal apparent negative interaction in competition for pollinator services. Mixed pollination with conspecific pollen and counterpart pollen reduced seed set in S. japonica, and hybrid progeny produced by mixed pollination were less than 20% as fertile compared to the pure species. The DNA genotyping results suggested the possibility of hybridization where their distributions overlap, and the genome-wide DNA analysis results showed clear genetic differentiation between the two species as well as the existence of hybrids. These results suggest that bi-directional reproductive interference between S. japonica and S. lutescens may have led to their present separated distributions at a small scale.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1033-1047"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Athyrium yokoscense, a cadmium-hypertolerant fern, exhibits two cadmium stress mitigation strategies in its roots and aerial parts. Athyrium yokoscense是一种耐镉蕨类植物，在其根部和气生部分表现出两种镉胁迫缓解策略。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.1007/s10265-024-01574-9

Yuko Ukai, Hiroki Taoka, Manaka Kamada, Yuko Wakui, Fumiyuki Goto, Kazuyoshi Kitazaki, Tomoko Abe, Akiko Hokura, Toshihiro Yoshihara, Hiroaki Shimada

{"title":"Athyrium yokoscense, a cadmium-hypertolerant fern, exhibits two cadmium stress mitigation strategies in its roots and aerial parts.","authors":"Yuko Ukai, Hiroki Taoka, Manaka Kamada, Yuko Wakui, Fumiyuki Goto, Kazuyoshi Kitazaki, Tomoko Abe, Akiko Hokura, Toshihiro Yoshihara, Hiroaki Shimada","doi":"10.1007/s10265-024-01574-9","DOIUrl":"10.1007/s10265-024-01574-9","url":null,"abstract":"Athyrium yokoscense is hypertolerant to cadmium (Cd) and can grow normally under a high Cd concentration despite Cd being a highly toxic heavy metal. To mitigate Cd stress in general plant species, Cd is promptly chelated with a thiol compound and is isolated into vacuoles. Generated active oxygen species (ROS) in the cytoplasm are removed by reduced glutathione. However, we found many differences in the countermeasures in A. yokoscense. Thiol compounds accumulated in the stele of the roots, although a long-term Cd exposure induced Cd accumulation in the aerial parts. Synchrotron radiation-based X-ray fluorescence (SR-XRF) analysis indicated that a large amount of Cd was localized in the cell walls of the roots. Overexpression of AyNramp5a, encoding a representative Fe and Mn transporter of A. yokoscense, increased both Cd uptake and Fe and Mn uptake in rice calli under the Cd exposure conditions. Organic acids are known to play a key role in reducing Cd availability to the plants by forming chelation and preventing its entry in free form into the roots. In A. yokoscense roots, Organic acids were abundantly detected. Investigating the chemical forms of the Cd molecules by X-ray absorption fine structure (XAFS) analysis detected many compounds with Cd-oxygen (Cd-O) binding in A. yokoscense roots, whereas in the aerial parts, the ratio of the compounds with Cd-sulfur (Cd-S) binding was increased. Together, our results imply that the strong Cd tolerance of A. yokoscense is an attribute of the following two mechanisms: Cd-O compound formation in the cell wall is a barrier to reduce Cd uptake into aerial parts. Thiol compounds in the region of root stele are involved in detoxication of Cd by formation of Cd-S compounds.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1019-1031"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemotaxonomy compared to morphological and anatomical taxonomy of five Hibiscus species. 将化学分类法与五个芙蓉品种的形态和解剖分类法进行比较。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-15 DOI: 10.1007/s10265-024-01566-9

Hala M E Abdelfattah, Hussein A Hussein, Samir S Teleb, Marwa M El-Demerdash, Nelly M George

{"title":"Chemotaxonomy compared to morphological and anatomical taxonomy of five Hibiscus species.","authors":"Hala M E Abdelfattah, Hussein A Hussein, Samir S Teleb, Marwa M El-Demerdash, Nelly M George","doi":"10.1007/s10265-024-01566-9","DOIUrl":"10.1007/s10265-024-01566-9","url":null,"abstract":"Hibiscus is a charismatic genus of the Malvaceae family that is noted for its diversity, lacking identifiable characteristics for distinguishing its various species. Therefore, there is an urgent need to develop authentication methods for genus delimitation and species delineation. The present study aims to discern the taxonomic relationships between the well-known, globally familiar, and economically important five Hibiscus species, namely: H. × rosa-sinensis, H. sabdariffa, H. schizopetalus, H. syriacus and H. tiliaceus based on traditional morphological and anatomical characteristics compared to the contemporary chemotaxonomy. In this context, the leaf-based methanolic extracts of the studied species were analyzed by Gas Chromatography-Mass Spectrometer (GC-MS) to estimate their secondary metabolites similarity. In addition, selected qualitative morphological and anatomical traits including leaf venation patterns, epidermal micromorphology, stomata types and trichomes diversity, petiole serial sectioning (outline, adaxial groove features, vasculature traces arrangement), and midrib characteristics of the studied species were investigated. The results of both chemotaxonomy and traditional taxonomy exhibited a remarkable agreement in the delineation of the five studied species. Specifically, the chemotaxonomy-based dendrogram separates the studied species into two main clusters with the H. sabdariffa as an outlier species in a single cluster and the remaining four species as another cluster with variant distances in its similarity indices. Similarly, the traditional morphological and anatomical characteristics revealed distinct traits for H. sabdariffa compared to the remaining four species. The findings of this study highlight the significance of integrating the structural features with phytochemicals profiling as a potential approach that could be harnessed for the delineation of the taxonomically challenging Hibiscus genus.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"967-984"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Network analyses show horizontal and vertical distribution of vascular epiphytes on their hosts in a fragment of cloud forest in Central Mexico. 网络分析显示了墨西哥中部云雾森林片段中维管束附生植物在其寄主上的水平和垂直分布。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-24 DOI: 10.1007/s10265-024-01569-6

Elizabeth Victoriano-Romero, Dulce María Figueroa-Castro, Jonas Morales-Linares

{"title":"Network analyses show horizontal and vertical distribution of vascular epiphytes on their hosts in a fragment of cloud forest in Central Mexico.","authors":"Elizabeth Victoriano-Romero, Dulce María Figueroa-Castro, Jonas Morales-Linares","doi":"10.1007/s10265-024-01569-6","DOIUrl":"10.1007/s10265-024-01569-6","url":null,"abstract":"Cloud forests figure as one of the most diverse ecosystems, accounting not only for a high number of plant species but also with a great variety of interactions among them. A common interaction in these forests is the one between vascular epiphytes and their hosts. However, few studies have used the network approach to analyze them. Here, we analyze the horizontal and vertical structure of the vascular epiphyte - host network in a cloud forest in central Mexico. We quantified the number of epiphyte stands on each host both total and per-stratum. Complete network, group, and species metrics were estimated at both levels of analysis. The host - epiphyte networks had relatively low network size but were highly connected; moderately nested, with low specialization, and modularity; but higher vulnerability than generality, and high niche overlap. The community was composed by a high number of generalist species. To our knowledge this is the first study in which network analyses are conducted with standardized data and including all host and epiphyte species in the community. The analyses suggest that the networks are robust, and that functional redundancy might be probable, two advantageous characteristics in a very fragmented and threatened cloud forest.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"985-995"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of laccase and ascorbate oxidase affects lignin composition in Arabidopsis thaliana stems. 漆酶和抗坏血酸氧化酶的表达会影响拟南芥茎中木质素的组成。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-10-07 DOI: 10.1007/s10265-024-01585-6

Konan Ishida, Senri Yamamoto, Takashi Makino, Yuki Tobimatsu

{"title":"Expression of laccase and ascorbate oxidase affects lignin composition in Arabidopsis thaliana stems.","authors":"Konan Ishida, Senri Yamamoto, Takashi Makino, Yuki Tobimatsu","doi":"10.1007/s10265-024-01585-6","DOIUrl":"10.1007/s10265-024-01585-6","url":null,"abstract":"Lignin is a phenolic polymer that is a major source of biomass. Oxidative enzymes, such as laccase and peroxidase, are required for lignin polymerisation. Laccase is a member of the multicopper oxidase family and has a high amino acid sequence similarity with ascorbate oxidase. However, the process of functional differentiation between the two enzymes remains poorly understood. In this study, the common ancestry sequence of laccase and ascorbate oxidase (AncMCO) was predicted via phylogenetic reconstruction, and its in vivo effect on lignin biosynthesis in Arabidopsis thaliana was assessed. The estimated AncMCO sequence conserved key residues that coordinate with copper ions, implying that the electron transfer system is likely to be conserved in AncMCO. However, multiple insertions/deletions corresponding to protein surface structures have been found between laccase, ascorbate oxidase, and AncMCO. The overexpression of canonical laccase (AtLAC4) and ascorbate oxidase (AtAAO1) in A. thaliana resulted in notable increases of syringyl/guaiacyl lignin unit ratio in stems, whereas, in contrast, the overexpression of AncMCO did not show any detectable change in lignin deposition. Transcriptomic analysis revealed that the AtAAO1-overexpressing line exhibited significant changes in the expression of a wide range of cell wall biosynthesis genes. These results highlight the importance of the molecular evolution of multicopper oxidase, which drives lignin biosynthesis during plant evolution.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1177-1187"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgement. 致谢。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 DOI: 10.1007/s10265-024-01589-2

引用次数: 0