Journal of Plant Research最新文献

{"title":"Floral and inflorescence ontogeny in the Brownea clade (Fabaceae: Detarioideae):insights into a morphologically diverse lineage of legumes.","authors":"Ana Tereza Novaes Parga Rodrigues, Marcus José de Azevedo Falcão, Vidal de Freitas Mansano","doi":"10.1007/s10265-026-01723-2","DOIUrl":"10.1007/s10265-026-01723-2","url":null,"abstract":"<p><p>The subfamily Detarioideae stands out among legumes as a clade with diverse floral morphology. Within it, the seven genera of the Brownea clade exhibit significant variability, especially in the merism of corolla and androecium, inflorescence architecture, and pollination strategies. This study aims to expand the understanding of the morphology and development of inflorescences and flowers of six species within the clade: Brownea longipedicellata, Brownea leucantha, Heterostemon mimosoides, Macrolobium suaveolens, Paloue paraensis and Paloue speciosa. We also provided an anatomical analysis of the nectariferous hypanthium of four of these species. We discuss the roles of heterochrony and mechanical pressures in floral development and how these factors lead to the observed morphological diversity. The study reveals several ontogenetic novelties for the Brownea clade, such as the basipetal development of B. longipedicellata racemes, the erratic organogenesis of the flowers of B. leucantha due to mechanical pressure from adjacent flowers in the congested inflorescences, the heteromorphic development of the corolla in H. mimosoides, and the development of massive bracteoles of M. suaveolens impacting the development of subsequent whorls. We provide new insights on the meristic reduction in different taxa within Detarioideae. In addition, we observed that in the species of the clade Heterostemon+Macrolobium+Paloue, the adaxial petal surrounds the carpel in the bud, a previously undescribed feature in Detarioideae, and possibly a new synapomorphy of this clade. We describe the inflorescence development in the group, which varies from simple racemes in most taxa to large capituliform racemes in Browneopsis and in some species of Brownea and Paloue. We compare this inflorescence and floral diversity, including the anatomy of the hypanthium with different pollination syndromes, including entomophily, ornithophily, and chiropterophily.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"805-840"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stylar curvature and its influence on the reproductive assurance in Passiflora foetida.","authors":"Sani Das, Marami Mili, Suman Paul, Badal Kumar Datta","doi":"10.1007/s10265-026-01745-w","DOIUrl":"10.1007/s10265-026-01745-w","url":null,"abstract":"<p><p>Reproductive assurance represents a key adaptive strategy in flowering plants, and many floral traits have evolved or been modified to ensure successful reproduction under environmental uncertainty. In Passiflora foetida L., styles exhibit varying degrees of curvature, giving rise to three distinct (fully curved, semi-curved, and erect) flower types during anthesis, each characterized by a different potential to ensure reproductive success. In this study, for the first time, the relationships among stylar curvature, pollen deposition, pollinator visitation, fruit set, and seed set were quantified under natural and controlled pollination treatments. The results showed that fully curved styles received the highest stigmatic pollen loads and exhibited the greatest fruit and seed set. Semi-curved flowers displayed intermediate reproductive success, whereas erect flowers recorded the lowest values across all reproductive parameters. The major pollinators, carpenter bees (Xylocopa spp.) and the giant honey bee (Apis dorsata), differed in visitation rates and single-visit effectiveness. However, pollination success depended strongly on stylar position. Stylar position played a decisive role in determining whether these visits resulted in successful fertilization. Notably, only fully curved flowers were capable of setting fruit through autonomous self-pollination, highlighting that stylar curvature serves as a mechanism of reproductive assurance under conditions of pollinator scarcity or unreliability.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"791-803"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From cavities to canals: evidence of developmental continuity in Myrsine guianensis (Primulaceae).","authors":"Silvia Rodrigues Machado, Zoraide Valério, Karla Bianca de Deus Bento, Yve Canaveze","doi":"10.1007/s10265-026-01732-1","DOIUrl":"10.1007/s10265-026-01732-1","url":null,"abstract":"<p><p>Secretory cavities and canals are traditionally treated as distinct anatomical categories and widely employed as diagnostic characters in taxonomic studies. However, their coexistence or the presence of intermediate forms in the same individual or organ points to a more intricate developmental relationship. The mechanisms underlying their formation and structural differentiation, particularly the boundaries between cavities and canals, remain insufficiently understood. In this study, we selected Myrsine guianensis (Aubl.) Kuntze (Primulaceae) because it displays globose and elliptical cavities alongside linear, canal-like secretory spaces. This condition raises a fundamental question: do these structures represent two discrete types of secretory spaces, or are they transitional forms along a developmental continuum? To address this question, we investigated the secretory spaces from a developmental point of view using light and transmission electron microscopy. Secretory cavities originated from the fundamental meristem through a schizolysigenous process, giving rise to a lumen lined by a uniseriate secretory epithelium and surrounded by one or two layers of sheath cells. Epithelial cells exhibited ultrastructural features indicative of intense metabolic activity associated with oil-resin synthesis. Secretion involved pronounced cell-wall remodeling, transient periplasmic spaces, and the replacement of senescent epithelial cells. A shift from resin- and phenolic-rich secretion to predominantly oil-rich secretion coincided with epithelial senescence and the recruitment of sheath cells, thereby sustaining secretory activity. The fusion of adjacent cavities, epithelial reorganization, and progressive lumen expansion produced transitional forms between globose and elongated structures, supporting the existence of a developmental cavity-canal continuum. This structural plasticity challenges the view of secretory spaces as discrete anatomical entities and suggests that spatial constraints during morphogenesis contribute to their diversification.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"841-854"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GsIRT3: a zinc-iron transporter gene from Glycine soja, enhancing alkali stress tolerance in Arabidopsis thaliana.","authors":"Deqiang Ding, Chengbo Zhang, Zaib-Un Nisa, Liankun Xu, Naila Ali, Anis Ali Shah, Xiaoxia Jin, Asfa Batool, Chao Chen, Mansour K Gatasheh","doi":"10.1007/s10265-026-01734-z","DOIUrl":"10.1007/s10265-026-01734-z","url":null,"abstract":"<p><p>ZIP family genes are known to play important roles in the transport of divalent metal ions such as zinc and iron. However, their roles in alkali stress have not been well characterized so far. In this study, a ZIP (Zrt, Irt-like Protein) cDNA was isolated from wild soybean (Glycine soja) by reverse transcription PCR (RT-PCR) and named as GsIRT3. GsIRT3 displayed differential gene expression levels at different time points under alkali treatment in both roots and leaves. Heterologous expression of GsIRT3 in yeast (pYES2-GsIRT3) conferred enhanced tolerance to both iron- and zinc-deficiency stress conditions. In addition, GsIRT3 overexpression lines (OX) of Arabidopsis thaliana were created by floral dip method for functional characterization of GsIRT3 under stress treatments. The results displayed that GsIRT3 OX lines performed better under alkali stress than wild-type (WT) plants in terms of higher root lengths and fresh biomass. Physiological indicator assays showed that OX lines appeared with higher chlorophyll, lower malondialdehyde (MDA), and H₂O₂ contents than WT plants under alkali stress. Further, catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) activities increased in OX lines under alkali stress. Superoxide radical accumulation was further evaluated by nitro-blue tetrazolium (NBT) staining, where WT plants exhibited more intense staining than the overexpression (OX) lines, indicating higher superoxide levels in WT. To further verify the role of GsIRT3 in stress mechanisms, expression levels of stress responsive marker genes (RD29A, COR15, KIN1, and H⁺-ATPase) were determined by qRT-PCR (Quantitative Real‑time PCR) analysis and all marker genes showed high transcript expression in OX lines after stress application compared to WT. Finally, functional characterization of GsIRT3 overexpression in soybean displayed better hairy root growth and increased fresh weight in OX lines compared to the soybean K599 line. These results clearly suggest the positive roles of GsIRT3 in alkali stress tolerance mechanisms.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"871-883"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Can floral preference of insect visitors be applied to species identification of the Japanese Arisaema (Araceae)?","authors":"Tetsuya K Matsumoto, Satoshi Kakishima, Tomiki Kobayashi, Nanako Maekawa, Kotone Tatano, Junichi Ohno, Muneto Hirobe, Masahiro Sueyoshi, Shungo Kariyama, Yudai Okuyama, Yuko Miyazaki","doi":"10.1007/s10265-026-01729-w","DOIUrl":"10.1007/s10265-026-01729-w","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"931"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of root microbiomes in four Swertia species from Taiwan.","authors":"Po-Yi Chen, Tsai-Wen Hsu, Tzen-Yuh Chiang, Chao-Li Huang","doi":"10.1007/s10265-026-01742-z","DOIUrl":"10.1007/s10265-026-01742-z","url":null,"abstract":"<p><p>Swertia (Gentianaceae) comprises four species endemic to Taiwan that possess significant medicinal potential. While root microbiomes are known to promote plant adaptation, the microbial ecology of Taiwanese Swertia remains largely unexplored. We investigated the rhizosphere and root endosphere microbiomes of these species using 16S rRNA gene sequencing and predictive functional profiling, integrated with host phylogenetic data. Our results revealed that rhizosphere bacterial communities were significantly more diverse than those in the root endosphere. PERMANOVA indicated that host species and plant compartment significantly influenced bacterial communities, but the high residual variance suggests that much of the community variation remains unexplained by the variables measured in this study. Phylogenetic analysis indicated that the root endosphere is more strongly influenced by host phylogeny, with closely related species harboring more similar communities. Functional profiling further demonstrated that the rhizosphere is predicted to be enriched in pathways related to nitrogen fixation and organic matter degradation, whereas the endosphere harbors bacterial taxa potentially associated with pathogen suppression. These findings underscore the multifaceted roles of the root microbiome in supporting the development, stress adaptation, and ecosystem sustainability of Swertia species in Taiwan's unique altitudinal gradients.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"771-790"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148604311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Habitat explains intraspecific leaf morphological and physiological variation in the facultative epiphyte Nephrolepis cordifolia.","authors":"Rui-Hong Hsu, J Aaron Hogan, Chiao-Ping Wang, Teng-Chiu Lin","doi":"10.1007/s10265-026-01737-w","DOIUrl":"10.1007/s10265-026-01737-w","url":null,"abstract":"<p><p>Epiphytic and terrestrial habitats differ markedly in water, nutrient, and light availability and variability, yet few facultative epiphytes successfully grow in both habitats. As a facultative epiphyte, Nephrolepis cordifolia provides a valuable opportunity to investigate how individuals of the same species adjust morphological and physiological traits along contrasting microhabitats. We measured frond structure, physiology, and tissue nutrient concentrations for 20 epiphytic and 20 terrestrial N. cordifolia individuals to evaluate intraspecific variation in resource-use strategies by growth habitat. Our findings show that not all Leaf Economic Spectrum traits covary in this species. Structurally, while specific leaf area, leaf dry matter content, and stomatal morphology were conserved across habitats, epiphytic individuals exhibited greater leaf thickness, a trait consistent with conservative leaf economics strategies. Functionally, epiphytes exhibited more enriched δ¹³C, indicating higher intrinsic water-use efficiency and tighter stomatal regulation. Terrestrial individuals displayed significantly higher maximum quantum yield of PSII (F_v/F_m) and greater mass-based chlorophyll concentrations, reflecting higher potential photosynthetic capacity. In terms of leaf tissue chemistry, terrestrial individuals had higher nitrogen and magnesium concentrations, while epiphytes showed higher leaf carbon content and significantly elevated C/N ratios. These findings highlight the physiological plasticity of N. cordifolia, which enables adjustment to contrasting microhabitats through adjustments in water-use efficiency, photosynthetic capacity, and leaf chemistry. Our results support the idea that epiphytic plants generally exhibit a more conservative physiological profile, with reduced F_v/F_m and enriched δ¹³C, showing how they may function under chronically constrained physiological margins. This vulnerability underscores the potential risks epiphytes face under intensifying drought and irradiance associated with climate change, raising important questions about the resilience of facultative epiphytes in increasingly variable environments.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"745-756"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unique variegation characteristics of camellia cultivar 'Kumagai', in which chloroplasts disappear only in palisade tissue.","authors":"Kento Terada, Chiyomi Uematsu, Toshihiro Yamada, Tomofumi Mochizuki, Riichi Oguchi","doi":"10.1007/s10265-026-01740-1","DOIUrl":"10.1007/s10265-026-01740-1","url":null,"abstract":"<p><p>Previous studies reported that some types of variegation are caused by reduction of chlorophyll and infection of pathogens, and the variegated leaves showed lower photosynthetic capacity. To elucidate the mechanisms that control the variegation, we observed a variegated individual of camellia cultivar 'Kumagai' in which leaves are variegated only on the adaxial side. We found for the first time in seed plants that the amount of chloroplasts was decreased only in palisade tissue while healthy chloroplasts were maintained in spongy tissue. Variegated parts of leaves of variegated plant of 'Kumagai' maintained high Fv/Fm value in spite of lacking chloroplasts in palisade tissue. It should be also noted that highly variegated leaves were concentrated on specific branches. We also confirmed that this variegated 'Kumagai' was infected by Camellia japonica-associated betaflexivirus (CJaBV), camellia ringspot associated virus (CRSaV) and camellia yellow ringspot virus (CaYRV). These unique characteristics of variegation in 'Kumagai' will provide good opportunities to study the physiological and molecular mechanisms of variegation in future research.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"757-770"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-flow liquid chromatography-mass spectrometry for highly sensitive quantification of indole-3-acetic acid from small tissue sections prepared by laser micro dissection.","authors":"Hiromi Suzuki, Yumiko Takebayashi, Shunsuke Watanabe, Noriko Takeda-Kamiya, Kiminori Toyooka, Masami Yokota Hirai, Mitsunori Seo","doi":"10.1007/s10265-026-01739-8","DOIUrl":"10.1007/s10265-026-01739-8","url":null,"abstract":"<p><p>Plant hormones play critical roles in many aspects of plant life cycles including development, growth, reproduction and responses to environmental stimuli. These processes are often associated with changes in endogenous plant hormone levels and locations. Therefore, to understand the modes of action of plant hormones, it is important to accurately quantify these chemical compounds in a high-definition tissue map. In this study, we developed a system to quantify indole-3-acetic acid (IAA), the major endogenous auxin, from small tissue samples using laser microdissection (LMD) coupled with nano-flow liquid chromatography (nano-LC)-mass spectrometry (MS), which improved detection limits, allowing quantification of IAA from a single 10 μm cryosection of maize coleoptile. Our results reveal that IAA is actively synthesized in the apical 400 μm region of the coleoptiles and is preferentially accumulated in vascular tissues. This technique can provide a precise view of the spatiotemporal distribution of plant hormones and their significance in regulating physiological responses at tissue or cellular levels.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"921-929"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PUCHI delimits the spatial domain of nodule organogenesis associated with auxin patterning and NIN-dependent transcription in Lotus japonicus.","authors":"Takashi Soyano, Yosuke Umehara, Makoto Hayashi, Masayoshi Kawaguchi","doi":"10.1007/s10265-026-01748-7","DOIUrl":"10.1007/s10265-026-01748-7","url":null,"abstract":"<p><p>Root nodule organogenesis requires the activation of symbiotic developmental programs within a spatially restricted region of the root. Although many regulators of nodule initiation have been identified, the mechanisms that delimit the domain of organogenic cell proliferation remain poorly understood. In this study, we show that the AP2/ERF transcription factors PUCHI1 and PUCHI2 function in spatially restricting cortical cell division during nodulation in Lotus japonicus. The loss of PUCHI function increased infection thread formation and nodule primordium initiation; however, it did not increase the number of mature nodules. Instead, puchi1 and puchi2 mutants frequently formed clustered nodules accompanied by ectopic cortical cell divisions surrounding developing primordia. Constitutive activation of CCaMK induced broadened spontaneous proliferative structures in the mutant background even in the absence of rhizobia, indicating that this phenotype was not simply a consequence of enhanced infection. PUCHI1 expression was induced during early symbiotic signalling downstream of the NODULE INCEPTION (NIN)-associated transcriptional network; both PUCHI genes were preferentially expressed in the basal region of developing primordia, corresponding to sites of ectopic proliferation in the mutant. Transcriptome and reporter analyses suggested that PUCHI regulated auxin-response patterning, potentially via STY1-, YUCCA11-, TAR2-, and Forked1-like-associated pathways. Additionally, PUCHI1 promoted NIN expression via the cytokinin-responsive CE region of the distal promoter independently of LHK1-mediated cytokinin perception. These findings indicate that PUCHI genes define the spatial domain of organogenic cell proliferation during nodulation; they coordinate auxin-related patterning with the NIN transcriptional module to ensure localised nodule formation.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"855-870"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}