Journal of Plant Research最新文献_第3页

Light promotes asexual reproduction and mediates transcriptomic changes in Pediastrum duplex. 光能促进无性繁殖，并介导双壳贝母的转录组变化。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-20 DOI: 10.1007/s10265-024-01567-8

Akari Masaki, Narumi Miyamoto, Sridharan Harshavardhini, Noriko Nagata, Yuki Tsuchikane, Hiroyuki Sekimoto, Yutaka Kodama, Tomohiro Suzuki, Tomoko Shinomura

引用次数: 0

Impaired inosine monophosphate dehydrogenase leads to plant-specific ribosomal stress responses in Arabidopsis thaliana. 单磷酸肌苷脱氢酶受损导致拟南芥植物特有的核糖体应激反应

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-09-05 DOI: 10.1007/s10265-024-01578-5

Shugo Maekawa, Ikuto Nishikawa, Gorou Horiguchi

{"title":"Impaired inosine monophosphate dehydrogenase leads to plant-specific ribosomal stress responses in Arabidopsis thaliana.","authors":"Shugo Maekawa, Ikuto Nishikawa, Gorou Horiguchi","doi":"10.1007/s10265-024-01578-5","DOIUrl":"10.1007/s10265-024-01578-5","url":null,"abstract":"Nucleotides are the building blocks of living organisms and their biosynthesis must be tightly regulated. Inosine monophosphate dehydrogenase (IMPDH) is a rate-limiting enzyme in GTP synthesis that is essential for biological activities, such as RNA synthesis. In animals, the suppression of IMPDH function causes ribosomal stress (also known as nucleolar stress), a disorder in ribosome biogenesis that results in cell proliferation defects and apoptosis. Despite its importance, plant IMPDH has not been analyzed in detail. Therefore, we analyzed the phenotypes of mutants of the two IMPDH genes in Arabidopsis thaliana and investigated their relationship with ribosomal stress. Double mutants of IMPDH1 and IMPDH2 were lethal, and only the impdh2 mutants showed growth defects and transient chlorophyll deficiency. These results suggested that IMPDH1 and IMPDH2 are redundant and essential, whereas IMPDH2 has a crucial role. In addition, the impdh2 mutants showed a reduction in nucleolus size and resistance to several translation inhibitors, which is a known response to ribosomal stress. Furthermore, the IMPDH1/impdh1 impdh2 mutants showed more severe growth defects and phenotypes such as reduced plastid rRNA levels and abnormal processing patterns than the impdh2 mutants. Finally, multiple mutations of impdh with as2, which has abnormal leaf polarity, caused the development of needle-like leaves because of the enhancement of the as2 phenotype, which is a typical effect observed in mutants of genes involved in ribosome biogenesis. These results indicated that IMPDH is closely related to ribosome biogenesis, and that mutations in the genes lead to not only known responses to ribosomal stress, but also plant-specific responses.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1091-1104"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of photosystems II and I depending on N partitioning to Rubisco in rice leaves: a study using Rubisco-antisense transgenic plants. 光系统 II 和 I 的调节取决于水稻叶片中 Rubisco 的氮分配：利用 Rubisco-antisense 转基因植物进行的研究。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-09-26 DOI: 10.1007/s10265-024-01582-9

Yuta Nakamura, Shinya Wada, Chikahiro Miyake, Amane Makino, Yuji Suzuki

{"title":"Regulation of photosystems II and I depending on N partitioning to Rubisco in rice leaves: a study using Rubisco-antisense transgenic plants.","authors":"Yuta Nakamura, Shinya Wada, Chikahiro Miyake, Amane Makino, Yuji Suzuki","doi":"10.1007/s10265-024-01582-9","DOIUrl":"10.1007/s10265-024-01582-9","url":null,"abstract":"We have previously suggested that in rice (Oryza sativa L.) leaves of different ages and N nutrition statuses, photosystems II and I (PSII and PSI, respectively) are regulated depending on N partitioning to Rubisco, which can determine the magnitude of unutilized light energy. The robustness of this mechanism was tested using Rubisco-antisense transgenic rice plants, in which reduced N partitioning to Rubisco markedly increases unutilized light energy. In wild-type plants, N partitioning to Rubisco tended to be smaller in the leaves at lower positions owing to leaf senescence. In the transgenic plants, N partitioning to Rubisco was generally smaller than in the wild-type plants and was relatively constant among leaf positions. The quantum efficiency of PSII [Y(II)] and quantum yield of non-photochemical quenching [Y(NPQ)] correlated positively and negatively, respectively, with N partitioning to Rubisco irrespective of leaf position or genotype. The oxidation levels of the reaction center chlorophyll of PSI (P700) [Y(ND)] negatively correlated with N partitioning to Rubisco. However, in mature and early senescent leaves of the transgenic plants, Y(ND) was markedly lower than expected from N partitioning to Rubisco. These results suggest that in the transgenic plants, the regulation depending on N partitioning to Rubisco is robust for PSII but fails for PSI in mature and early senescing leaves. In these leaves, the magnitudes of P700 oxidation were found to be less than expected from the Y(II) and Y(NPQ) values. The mechanistic reasons and physiological implications of these phenomena are discussed.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1165-1175"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

East Asian forest-steppe outpost in the Khanka Lowland (Russia) and its conservation. 俄罗斯汉卡低地的东亚森林草原前哨及其保护。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-24 DOI: 10.1007/s10265-024-01570-z

Elena A Marchuk, Anastasiya K Kvitchenko, Lyubov A Kameneva, Aleksandra A Yuferova, Dmitry E Kislov

{"title":"East Asian forest-steppe outpost in the Khanka Lowland (Russia) and its conservation.","authors":"Elena A Marchuk, Anastasiya K Kvitchenko, Lyubov A Kameneva, Aleksandra A Yuferova, Dmitry E Kislov","doi":"10.1007/s10265-024-01570-z","DOIUrl":"10.1007/s10265-024-01570-z","url":null,"abstract":"The Khanka Lowland forest-steppe is the most eastern outpost of the Eurasian steppe biome. It includes unique grassland plant communities with rare steppe species. These coenosis have changed under the influence of anthropogenic activity, especially during the last 100 years and included both typical steppe species and nemoral mesophytic species. To distinguish these ecological groups of plants the random forest method with three datasets of environmental variables was applied. Specifically, a model of classification with the most important bioindices to predict a mesophytic ecological group of plants with a sensitivity greater than 80% was constructed. The data demonstrated the presence of steppe species that arrived at different times in the Primorye Territory. Most of these species are associated with the Mongolian-Daurian relict steppe complex and habit in the Khanka Lowland. Other species occur only in mountains in Primorye Territory and do not persist in the Khanka Lowland. These findings emphasize the presence of relict steppe communities with a complex of true steppe species in the Khanka Lowland. Steppe communities exhibit features of anthropogenic influence definitely through the long land use period but are not anthropogenic in origin. The most steppe species are located at the eastern border of distribution in the Khanka Lowlands and are valuable in terms of conservation and sources of information about steppe species origin and the emergence of the steppe biome as a whole.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"997-1018"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A resilient mutualistic interaction between cucumber mosaic virus and its natural host to adapt to an excess zinc environment and drought stress. 黄瓜花叶病毒与其自然宿主之间为适应过量锌环境和干旱胁迫而产生的一种顽强的互利互动关系。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-27 DOI: 10.1007/s10265-024-01573-w

Midori Tabara, Shimpei Uraguchi, Masako Kiyono, Izumi Watanabe, Atsushi Takeda, Hideki Takahashi, Toshiyuki Fukuhara

{"title":"A resilient mutualistic interaction between cucumber mosaic virus and its natural host to adapt to an excess zinc environment and drought stress.","authors":"Midori Tabara, Shimpei Uraguchi, Masako Kiyono, Izumi Watanabe, Atsushi Takeda, Hideki Takahashi, Toshiyuki Fukuhara","doi":"10.1007/s10265-024-01573-w","DOIUrl":"10.1007/s10265-024-01573-w","url":null,"abstract":"A perennial pseudometallophyte Arabidopsis halleri is frequently infected with cucumber mosaic virus (CMV) in its natural habitat. The purpose of this study was to characterize the effect of CMV infection on the environmental adaptation of its natural host A. halleri. The CMV(Ho) strain isolated from A. halleri was inoculated into clonal virus-free A. halleri plants, and a unique plant-virus system consisting of CMV(Ho) and its natural wild plant host was established. In a control environment with ambient zinc supplementation, CMV(Ho) infection retarded growth in the above-ground part of host plants but conferred strong drought tolerance. On the other hand, in an excess zinc environment, simulating a natural edaphic environment of A halleri, host plants hyperaccumulated zinc and CMV(Ho) infection did not cause any symptoms to host plants while conferring mild drought tolerance. We also demonstrated in Nicotiana benthamiana as another host that similar effects were induced by the combination of excess zinc and CMV(Ho) infection. Transcriptomic analysis indicated that the host plant recognized CMV(Ho) as a mutualistic symbiont rather than a parasitic pathogen. These results suggest a resilient mutualistic interaction between CMV(Ho) and its natural host A. halleri in its natural habitat.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1151-1164"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of seed germination, seedling growth and physiological characteristics to alkali stress in halophyte Suaeda liaotungensis. 盐生植物 Suaeda liaotungensis 的种子萌发、幼苗生长和生理特征对碱胁迫的响应。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-28 DOI: 10.1007/s10265-024-01568-7

Jieqiong Song, Lantong Zhao, Yiming Ma, Xiaoqi Cao, Ruixuan An, Jingying Zhao, Haoran Ding, Hongfei Wang, Changping Li, Qiuli Li

{"title":"Response of seed germination, seedling growth and physiological characteristics to alkali stress in halophyte Suaeda liaotungensis.","authors":"Jieqiong Song, Lantong Zhao, Yiming Ma, Xiaoqi Cao, Ruixuan An, Jingying Zhao, Haoran Ding, Hongfei Wang, Changping Li, Qiuli Li","doi":"10.1007/s10265-024-01568-7","DOIUrl":"10.1007/s10265-024-01568-7","url":null,"abstract":"Soil salinization has been considered as a major environmental threat to plant growth. Different types of salt in saline soil have different effects on germination and seedling growth. Effect of NaCl on germination and seedling establishment in Suaeda liaotungensis have been reported, but its response to alkali stress remains unclear. Our results showed that brown seeds had higher germination rate, however, black seeds had higher germination recovery percentage under alkali stress. Na2CO3 had stronger inhibitory effect on germination and seedling growth than NaHCO3. As the concentration of alkali stress increased, the ROS level of brown seeds gradually ascended, while that of black seeds decreased first and then ascended. MDA content of dimorphic seeds significantly increased under alkali stress. The trend of SOD, POD and CAT activity between dimorphic seeds was similar under the same type of alkali stress. Alkali stress enhanced proline content of dimorphic seeds, and dimorphic seeds in NaHCO3 solution had higher proline content than Na2CO3 solution. Moreover, radicle and shoot tolerance indexes of seedlings in NaHCO3 solution were significantly higher than that of Na2CO3 solution. Under strong alkali stress, seedlings in NaHCO3 solution had significantly lower ROS level and MDA content as well as higher antioxidant enzyme activity than Na2CO3 solution. This study comprehensively compared the morphological and physiological characteristics in germination and seedlings to better reveal the saline-alkali tolerance mechanisms in S. liaotungensis.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1137-1149"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142093552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Critical stages in pea photosynthesis impaired by tetracycline as an environmental contaminant. 环境污染物四环素影响豌豆光合作用的关键阶段

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-09-21 DOI: 10.1007/s10265-024-01580-x

Magdalena Krupka, Dariusz J Michalczyk, Agnieszka I Piotrowicz-Cieślak

{"title":"Critical stages in pea photosynthesis impaired by tetracycline as an environmental contaminant.","authors":"Magdalena Krupka, Dariusz J Michalczyk, Agnieszka I Piotrowicz-Cieślak","doi":"10.1007/s10265-024-01580-x","DOIUrl":"10.1007/s10265-024-01580-x","url":null,"abstract":"The widespread use of antibiotics in intensive animal husbandry, and the agricultural utilization of manure from such farms, imposes a significant burden on the environment. Consequently, the effects of antibiotics should be studied not only in animals and humans but also in all components of biocenoses and agrocenoses. In our study, we analyze the impact of four different concentrations of tetracycline present in soil (0, 5, 50, and 500 mg/kg of soil) on the growth and key photosynthesis parameters of pea seedlings: chlorophyll concentration, aminolevulinic acid concentration, aminolevulinic acid dehydrogenase activity, and ribulose bisphosphate carboxylase-oxygenase (RuBisCO) activity. At the lowest tetracycline concentration, chlorophyll content decreased by 13% compared to the control (0 tetracycline), while at the highest antibiotic concentration, it decreased by as much as 27%. Similarly, the decrease in aminolevulinic acid (a chlorophyll precursor) concentration was significant, amounting to 34%. However, the activity of the dehydrogenase enzyme, which consumes this precursor, decreased even more drastically by 51%, indicating significant disturbances in the light phase of photosynthesis. However, the activity of RuBisCO in pea plants subjected to tetracycline was even more severely affected, dropping by 58%, 69%, and 70% in soils with increasing concentrations of tetracycline. The reduction in enzyme activity could only partially be explained by a less pronounced decrease in the quantity of RuBisCO (large subunit) protein, which amounted to 6.5%, 11%, and 35% for tetracycline concentrations of 5, 50, and 500 mg/kg of soil, respectively.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1049-1059"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotype identification and genome-wide association study of ear-internode vascular bundles in maize (Zea mays). 玉米（Zea mays）穗间维管束的表型鉴定和全基因组关联研究。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-07 DOI: 10.1007/s10265-024-01565-w

Huan Zhao, Ying Zhang, Xianju Lu, Yanxin Zhao, Chuanyu Wang, Weiliang Wen, Minxiao Duan, Shuaihao Zhao, Jinglu Wang, Xinyu Guo

{"title":"Phenotype identification and genome-wide association study of ear-internode vascular bundles in maize (Zea mays).","authors":"Huan Zhao, Ying Zhang, Xianju Lu, Yanxin Zhao, Chuanyu Wang, Weiliang Wen, Minxiao Duan, Shuaihao Zhao, Jinglu Wang, Xinyu Guo","doi":"10.1007/s10265-024-01565-w","DOIUrl":"10.1007/s10265-024-01565-w","url":null,"abstract":"The vascular bundle in the ear-internode of maize is a key conduit for transporting photosynthetic materials between \"source\" and \"sink\", making it critically important to examine its micro-phenotypes and genetic architecture to identify advantageous characteristics and cultivate high-yielding and high-quality varieties. Unfortunately, the limited observation methods and scope of study precludes any comprehensive and systematic investigations into the microscopic phenotypes and genetic mechanisms of vascular bundle in maize ear-internode. In this study, 47 phenotypic traits were extracted in 495 maize inbred lines using micro computed tomography (Micro-CT) scanning technology and a deep learning-based phenotype acquisition method for stem vascular bundle, which included stem slice-related, epidermis zone-related, periphery zone-related, inner zone-related and vascular bundles-related traits. Phenotypic analysis indicated that there was extensive phenotypic variation of vascular bundle traits in ear-internode, especially that in the inner zone. Of these, 30 phenotypic traits with heritability greater than 0.70 were conducted for GWAS, and a total of 4,225 significant SNPs and 416 candidate genes with detailed functional annotations were identified. Furthermore, 20 genes were highly expressed in stem-related tissues, especially in maize internodes. Functional analysis of candidate genes indicated that the pathways obtained for candidate genes of different trait groups were distinct, mainly involved in vitamin synthesis and metabolism, transport of substances, carbohydrate derivative catabolic process, protein transport and localization, and anatomical structure development. The results of this study will help to further understand the phenotypic traits of stem vascular bundles and provide a reference for revealing the genetic mechanism of maize ear-internode vascular bundles.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1073-1090"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RAL6 encodes a seed allergenic protein that positively regulates grain weight and seed germination. RAL6 编码一种种子致敏蛋白，它对谷物重量和种子萌发有积极的调节作用。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.1007/s10265-024-01581-w

Xin Yan, Wei Zhou, Xirui Huang, Jiexiu Ouyang, Shaobo Li, Jiadong Gao, Xin Wang

{"title":"RAL6 encodes a seed allergenic protein that positively regulates grain weight and seed germination.","authors":"Xin Yan, Wei Zhou, Xirui Huang, Jiexiu Ouyang, Shaobo Li, Jiadong Gao, Xin Wang","doi":"10.1007/s10265-024-01581-w","DOIUrl":"10.1007/s10265-024-01581-w","url":null,"abstract":"The rice albumin (RAG) gene family belongs to the Tryp_alpha_amyl family. RAG2, specifically expressed in 14-21 DAP (days after pollination) seeds, regulates grain yield and quality. In this study, we identified another RAG family gene, RAL6, which exhibits specific expression in developing seeds, particularly in 7, 10, and 15 DAP seeds. Employing the CRISPR/Cas9 system, we analyzed functions of RAL6 and found that the ral6 lines (ral6-1, ral6-2, ral6-3, and ral6-4) displayed thinner seeds with significantly decreased 1000-grain weight and grain thickness compared to ZH11. Additionally, the cell width of spikelet cells, total protein and glutelin contents were significantly reduced in ral6. The germination assay and 1% TTC staining revealed a significant decrease in seed vigor among the ral6 lines. The alpha-amylase activity in ral6 mutant seeds was also markedly lower than in ZH11 seeds after 2 days of imbibition. Furthermore, co-expression analysis and GO annotation showed that co-expressed genes were involved in immune response, oligopeptide transport, and the glucan biosynthetic process. Collectively, our findings suggest that RAL6 plays a coordinating role in regulating grain weight and seed germination in rice.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1105-1114"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SENSITIVE TO FREEZING2 is crucial for growth of Marchantia polymorpha under acidic conditions. SENSITIVE TO FREEZING2 对多孔菌在酸性条件下的生长至关重要。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-04 DOI: 10.1007/s10265-024-01564-x

Shinsuke Shimizu, Koichi Hori, Kimitsune Ishizaki, Hiroyuki Ohta, Mie Shimojima

{"title":"SENSITIVE TO FREEZING2 is crucial for growth of Marchantia polymorpha under acidic conditions.","authors":"Shinsuke Shimizu, Koichi Hori, Kimitsune Ishizaki, Hiroyuki Ohta, Mie Shimojima","doi":"10.1007/s10265-024-01564-x","DOIUrl":"10.1007/s10265-024-01564-x","url":null,"abstract":"Land plants have evolved many systems to adapt to a wide range of environmental stresses. In seed plants, oligogalactolipid synthesis is involved in tolerance to freezing and dehydration, but it has not been analyzed in non-vascular plants. Here we analyzed trigalactosyldiacylglycerol (TGDG) synthesis in Marchantia polymorpha. TGDG is synthesized by galactolipid: galactolipid galactosyltransferase [GGGT; SENSITIVE TO FREEZING2 (SFR2) in Arabidopsis]. We analyzed the subcellular localization and GGGT activity of two M. polymorpha SFR2 homologs (MpGGGT1 and MpGGGT2, each as a GFP-fusion protein) using a transient expression system in Nicotiana benthamiana leaves and found that MpGGGT1-GFP localized in the chloroplast envelope membrane. We produced mutants Mpgggt1 and Mpgggt2 and found that TGDG did not accumulate in Mpgggt1 upon treatment of the thallus with acetic acid. Moreover, growth of Mpgggt1 mutants was impaired by acetic acid treatment. Microscopy revealed that the acetic acid treatment of M. polymorpha plants damaged intracellular membranes. The fact that the effect was similar for wild-type and Mpgggt1 plants suggested that MpGGGT has a role in recovery from damage. These results indicate that MpGGGT plays a crucial role in M. polymorpha growth under conditions of acid stress, which may have been encountered during the ancient terrestrial colonization of plants.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1115-1126"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0