Journal of Plant Research最新文献_第3页

New insights into the evolution and function of the UMAMIT (USUALLY MULTIPLE ACIDS MOVE IN AND OUT TRANSPORTER) gene family. 对 UMAMIT（USUALLY MULTIPLE ACIDS MOVE IN AND OUT TRANSPORTER）基因家族的进化和功能的新认识。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-12 DOI: 10.1007/s10265-024-01596-3

Chenhao Cao, Xinbao Qiu, Zhongnan Yang, Yue Jin

{"title":"New insights into the evolution and function of the UMAMIT (USUALLY MULTIPLE ACIDS MOVE IN AND OUT TRANSPORTER) gene family.","authors":"Chenhao Cao, Xinbao Qiu, Zhongnan Yang, Yue Jin","doi":"10.1007/s10265-024-01596-3","DOIUrl":"https://doi.org/10.1007/s10265-024-01596-3","url":null,"abstract":"UMAMIT proteins have been known as key players in amino acid transport. In Arabidopsis, functions of several UMAMITs have been characterized, but their precise mechanism, evolutionary history and functional divergence remain elusive. In this study, we conducted phylogenetic analysis of the UMAMIT gene family across key species in the evolutionary history of plants, ranging from algae to angiosperms. Our findings indicate that UMAMIT proteins underwent a substantial expansion from algae to angiosperms, accompanied by the stabilization of the EamA (the main domain of UMAMIT) structure. Phylogenetic studies suggest that UMAMITs may have originated from green algae and be divided into four subfamilies. These proteins first diversified in bryophytes and subsequently experienced gene duplication events in seed plants. Subfamily I was potentially associated with amino acid transport in seeds. Regarding subcellular localization, UMAMITs were predominantly localized in the plasma membrane and chloroplasts. However, members from clade 8 in subfamily III exhibited specific localization in the tonoplast. These members may have multiple functions, such as plant disease resistance and root development. Furthermore, our protein structure prediction revealed that the four-helix bundle motif is crucial in controlling the UMAMIT switch for exporting amino acid. We hypothesize that the specific amino acids in the amino acid binding region determine the type of amino acids being transported. Additionally, subfamily II contains genes that are specifically expressed in reproductive organs and roots in angiosperms, suggesting neofunctionalization. Our study highlights the evolutionary complexity of UMAMITs and underscores their crucial role in the adaptation and diversification of seed plants.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Why can Palhinhaea cernua (lycophyte) grow closer to fumaroles in highly acidic solfatara fields? 在高酸性的溶岩田中，为什么蕨类植物 Palhinhaea cernua（狼尾草属植物）可以生长在离熔岩更近的地方？

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-11 DOI: 10.1007/s10265-024-01587-4

Toshihiro Watanabe, Nozomi Imai, Syuntaro Hiradate, Hayato Maruyama, Jun Wasaki

{"title":"Why can Palhinhaea cernua (lycophyte) grow closer to fumaroles in highly acidic solfatara fields?","authors":"Toshihiro Watanabe, Nozomi Imai, Syuntaro Hiradate, Hayato Maruyama, Jun Wasaki","doi":"10.1007/s10265-024-01587-4","DOIUrl":"https://doi.org/10.1007/s10265-024-01587-4","url":null,"abstract":"Palhinhaea cernua, a lycophyte, and Dicranopteris linearis, a fern, are commonly observed in solfatara fields in Kyushu, Japan, but their distribution trends are different. The aim of this study was to determine why P. cernua is more abundant in areas closer to fumaroles from both a soil and plant perspective. Samples of P. cernua and D. linearis, as well as their respective growing soils, were collected, and the mineral properties, including the concentration of various mineral elements and inorganic anions and δ15N, were determined. P. cernua was better adapted to soil with lower pH, higher soluble aluminum concentrations, and poorer calcium and phosphorus concentrations than D. linearis. A positive correlation was observed between shoot nitrogen concentration and both shoot sulfur concentration and soil water-soluble sulfur concentration in P. cernua, implying the involvement of sulfur in nitrogen acquisition in P. cernua. The results also suggested that D. linearis mainly uses soil NO3-N, while P. cernua uses NH4-N, which is predominant and excessive in the solfatara fields, particularly near the fumaroles. This high preference for NH4-N in P. cernua was confirmed through a cultivation experiment. While D. linearis prefers NO3-N and distributes further from fumaroles, P. cernua may have survived in the solfatara fields by utilizing NH4-N and sulfur, which are abundant near fumaroles where competition from other plant species is minimal.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil properties, climate, and topography jointly determine plant community characteristics in marsh wetlands. 土壤特性、气候和地形共同决定了沼泽湿地的植物群落特征。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-08 DOI: 10.1007/s10265-024-01593-6

Lin Yuan, Jingzhi Wang, Rong Liu, Yuqi Tang, Di Wu, Ri Jin, Weihong Zhu

{"title":"Soil properties, climate, and topography jointly determine plant community characteristics in marsh wetlands.","authors":"Lin Yuan, Jingzhi Wang, Rong Liu, Yuqi Tang, Di Wu, Ri Jin, Weihong Zhu","doi":"10.1007/s10265-024-01593-6","DOIUrl":"https://doi.org/10.1007/s10265-024-01593-6","url":null,"abstract":"Various environmental conditions influence the characteristics of plant communities within wetlands. Although the influence of key environmental factors on plant community traits within specific types of wetland ecosystems has been studied extensively, how they regulate plant communities across marsh wetland types remains poorly understood. We examined how environmental conditions influence plant communities in marsh wetlands along the lower Tumen River in northeastern China. We collected and analyzed data on the plant community characteristics (species, height, and coverage), soil physicochemical properties (organic carbon, inorganic nitrogen, and sulfur), and climatic and topographic factors (temperature, precipitation, and elevation) of 56 distinct marsh plots (29 herbaceous, 14 shrub, and 13 forested marshes) to understand how these variables correlate with plant community characteristics across marsh types. The wetland plant diversity varied, with the lowest, intermediate, and highest diversity occurring in herbaceous, shrub, and forested marshes, respectively. Climate, topography, and soil properties had crucial influences on plant diversity and biomass. Structural equation modeling showed that, in herbaceous marshes, plant biomass was primarily determined by soil and plant diversity, with climate exerting an indirect effect. In shrub marshes, soil, climate, and plant diversity directly influenced biomass. In forest marshes, soil and plant diversity directly affected biomass, whereas climate and topography had indirect effects. These findings highlight the complex interactions among environmental factors across marsh ecosystems and their influence mechanisms on biomass, aiding in formulating effective conservation and restoration strategies for marsh wetland ecosystems.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical genetics analysis suggests the involvement of Aurora kinase and MAPKs in aluminum-induced malate secretion in Arabidopsis. 化学遗传学分析表明，拟南芥中的极光激酶和 MAPK 参与了铝诱导的苹果酸盐分泌。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-06 DOI: 10.1007/s10265-024-01594-5

Liujie Wu, Liuying Lai, Weijun Wu, Yongzhuang Wang, Ganhui Mo, Yuriko Kobayashi, Naohisa Ogo, Hiroyuki Koyama

{"title":"Chemical genetics analysis suggests the involvement of Aurora kinase and MAPKs in aluminum-induced malate secretion in Arabidopsis.","authors":"Liujie Wu, Liuying Lai, Weijun Wu, Yongzhuang Wang, Ganhui Mo, Yuriko Kobayashi, Naohisa Ogo, Hiroyuki Koyama","doi":"10.1007/s10265-024-01594-5","DOIUrl":"https://doi.org/10.1007/s10265-024-01594-5","url":null,"abstract":"Chemical genetics is a multidisciplinary research method. In this study, it is used to screen compounds that promote aluminum-induced malate secretion in Arabidopsis thaliana. Inhibition of p38 mitogen-activated protein kinase (p38 MAPK; LY2228820) significantly increased the transcription of Arabidopsis thaliana aluminum-activated malate transporter 1 (AtALMT1) and sensitive to proton rhizotoxicity 1 (STOP1)-regulated genes, multidrug and toxic compound extrusion and aluminum sensitive 3, but not AtSTOP1 and the Al-biomarker genes At3g28510, At5g13320, suggesting that LY2228820 increased the early expression of STOP1-regulated genes without affecting AtSTOP1 expression. Inhibition of p38 MAPK (LY2228820) and Aurora A (MLN8237) increased aluminum-activated malate transport via AtALMT1, suggesting that both MLN8237 and LY2228820 interfere with AtALMT1 activity. An increase in root elongation was also observed in Arabidopsis after applying compounds LY2228820 and MLN8237. Thus, both LY2228820 and MLN8237 may play important roles in alleviating the inhibitory effects of aluminum on roots.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of reproductive interference between closely related Salvia species with small-scale separated distributions by multifaceted pollination and molecular analyses. 通过多方面的授粉和分子分析，检测小范围内分布分离的近缘丹参物种之间的生殖干扰。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-30 DOI: 10.1007/s10265-024-01577-6

Sachiko Nishida, Atsuko Takano, Yoshihisa Suyama, Satoshi Kakishima

{"title":"Detection of reproductive interference between closely related Salvia species with small-scale separated distributions by multifaceted pollination and molecular analyses.","authors":"Sachiko Nishida, Atsuko Takano, Yoshihisa Suyama, Satoshi Kakishima","doi":"10.1007/s10265-024-01577-6","DOIUrl":"10.1007/s10265-024-01577-6","url":null,"abstract":"Reproductive interference, an interspecific interaction in reproductive process that exerts an adverse effect, has gained attention as a contributing factor in promoting exclusive distributions between closely related species. However, detailed studies on the possibility of reproductive interference between native plants are still lacking, presumably because strong reproductive interference can rapidly realize exclusive distributions, leaving the two species apparently independent. Salvia japonica and S. lutescens are found in separate localities at a small scale, although their distributions overlap at a large scale. We investigated the possibility of reproductive interference between them through field surveys, hand-pollination experiments, evaluation of hybrid fertility, cpDNA and nrDNA genotyping, and genome-wide DNA analysis. The field survey results did not reveal apparent negative interaction in competition for pollinator services. Mixed pollination with conspecific pollen and counterpart pollen reduced seed set in S. japonica, and hybrid progeny produced by mixed pollination were less than 20% as fertile compared to the pure species. The DNA genotyping results suggested the possibility of hybridization where their distributions overlap, and the genome-wide DNA analysis results showed clear genetic differentiation between the two species as well as the existence of hybrids. These results suggest that bi-directional reproductive interference between S. japonica and S. lutescens may have led to their present separated distributions at a small scale.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1033-1047"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Athyrium yokoscense, a cadmium-hypertolerant fern, exhibits two cadmium stress mitigation strategies in its roots and aerial parts. Athyrium yokoscense是一种耐镉蕨类植物，在其根部和气生部分表现出两种镉胁迫缓解策略。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.1007/s10265-024-01574-9

Yuko Ukai, Hiroki Taoka, Manaka Kamada, Yuko Wakui, Fumiyuki Goto, Kazuyoshi Kitazaki, Tomoko Abe, Akiko Hokura, Toshihiro Yoshihara, Hiroaki Shimada

{"title":"Athyrium yokoscense, a cadmium-hypertolerant fern, exhibits two cadmium stress mitigation strategies in its roots and aerial parts.","authors":"Yuko Ukai, Hiroki Taoka, Manaka Kamada, Yuko Wakui, Fumiyuki Goto, Kazuyoshi Kitazaki, Tomoko Abe, Akiko Hokura, Toshihiro Yoshihara, Hiroaki Shimada","doi":"10.1007/s10265-024-01574-9","DOIUrl":"10.1007/s10265-024-01574-9","url":null,"abstract":"Athyrium yokoscense is hypertolerant to cadmium (Cd) and can grow normally under a high Cd concentration despite Cd being a highly toxic heavy metal. To mitigate Cd stress in general plant species, Cd is promptly chelated with a thiol compound and is isolated into vacuoles. Generated active oxygen species (ROS) in the cytoplasm are removed by reduced glutathione. However, we found many differences in the countermeasures in A. yokoscense. Thiol compounds accumulated in the stele of the roots, although a long-term Cd exposure induced Cd accumulation in the aerial parts. Synchrotron radiation-based X-ray fluorescence (SR-XRF) analysis indicated that a large amount of Cd was localized in the cell walls of the roots. Overexpression of AyNramp5a, encoding a representative Fe and Mn transporter of A. yokoscense, increased both Cd uptake and Fe and Mn uptake in rice calli under the Cd exposure conditions. Organic acids are known to play a key role in reducing Cd availability to the plants by forming chelation and preventing its entry in free form into the roots. In A. yokoscense roots, Organic acids were abundantly detected. Investigating the chemical forms of the Cd molecules by X-ray absorption fine structure (XAFS) analysis detected many compounds with Cd-oxygen (Cd-O) binding in A. yokoscense roots, whereas in the aerial parts, the ratio of the compounds with Cd-sulfur (Cd-S) binding was increased. Together, our results imply that the strong Cd tolerance of A. yokoscense is an attribute of the following two mechanisms: Cd-O compound formation in the cell wall is a barrier to reduce Cd uptake into aerial parts. Thiol compounds in the region of root stele are involved in detoxication of Cd by formation of Cd-S compounds.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1019-1031"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemotaxonomy compared to morphological and anatomical taxonomy of five Hibiscus species. 将化学分类法与五个芙蓉品种的形态和解剖分类法进行比较。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-15 DOI: 10.1007/s10265-024-01566-9

Hala M E Abdelfattah, Hussein A Hussein, Samir S Teleb, Marwa M El-Demerdash, Nelly M George

{"title":"Chemotaxonomy compared to morphological and anatomical taxonomy of five Hibiscus species.","authors":"Hala M E Abdelfattah, Hussein A Hussein, Samir S Teleb, Marwa M El-Demerdash, Nelly M George","doi":"10.1007/s10265-024-01566-9","DOIUrl":"10.1007/s10265-024-01566-9","url":null,"abstract":"Hibiscus is a charismatic genus of the Malvaceae family that is noted for its diversity, lacking identifiable characteristics for distinguishing its various species. Therefore, there is an urgent need to develop authentication methods for genus delimitation and species delineation. The present study aims to discern the taxonomic relationships between the well-known, globally familiar, and economically important five Hibiscus species, namely: H. × rosa-sinensis, H. sabdariffa, H. schizopetalus, H. syriacus and H. tiliaceus based on traditional morphological and anatomical characteristics compared to the contemporary chemotaxonomy. In this context, the leaf-based methanolic extracts of the studied species were analyzed by Gas Chromatography-Mass Spectrometer (GC-MS) to estimate their secondary metabolites similarity. In addition, selected qualitative morphological and anatomical traits including leaf venation patterns, epidermal micromorphology, stomata types and trichomes diversity, petiole serial sectioning (outline, adaxial groove features, vasculature traces arrangement), and midrib characteristics of the studied species were investigated. The results of both chemotaxonomy and traditional taxonomy exhibited a remarkable agreement in the delineation of the five studied species. Specifically, the chemotaxonomy-based dendrogram separates the studied species into two main clusters with the H. sabdariffa as an outlier species in a single cluster and the remaining four species as another cluster with variant distances in its similarity indices. Similarly, the traditional morphological and anatomical characteristics revealed distinct traits for H. sabdariffa compared to the remaining four species. The findings of this study highlight the significance of integrating the structural features with phytochemicals profiling as a potential approach that could be harnessed for the delineation of the taxonomically challenging Hibiscus genus.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"967-984"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Network analyses show horizontal and vertical distribution of vascular epiphytes on their hosts in a fragment of cloud forest in Central Mexico. 网络分析显示了墨西哥中部云雾森林片段中维管束附生植物在其寄主上的水平和垂直分布。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-08-24 DOI: 10.1007/s10265-024-01569-6

Elizabeth Victoriano-Romero, Dulce María Figueroa-Castro, Jonas Morales-Linares

{"title":"Network analyses show horizontal and vertical distribution of vascular epiphytes on their hosts in a fragment of cloud forest in Central Mexico.","authors":"Elizabeth Victoriano-Romero, Dulce María Figueroa-Castro, Jonas Morales-Linares","doi":"10.1007/s10265-024-01569-6","DOIUrl":"10.1007/s10265-024-01569-6","url":null,"abstract":"Cloud forests figure as one of the most diverse ecosystems, accounting not only for a high number of plant species but also with a great variety of interactions among them. A common interaction in these forests is the one between vascular epiphytes and their hosts. However, few studies have used the network approach to analyze them. Here, we analyze the horizontal and vertical structure of the vascular epiphyte - host network in a cloud forest in central Mexico. We quantified the number of epiphyte stands on each host both total and per-stratum. Complete network, group, and species metrics were estimated at both levels of analysis. The host - epiphyte networks had relatively low network size but were highly connected; moderately nested, with low specialization, and modularity; but higher vulnerability than generality, and high niche overlap. The community was composed by a high number of generalist species. To our knowledge this is the first study in which network analyses are conducted with standardized data and including all host and epiphyte species in the community. The analyses suggest that the networks are robust, and that functional redundancy might be probable, two advantageous characteristics in a very fragmented and threatened cloud forest.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"985-995"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of laccase and ascorbate oxidase affects lignin composition in Arabidopsis thaliana stems. 漆酶和抗坏血酸氧化酶的表达会影响拟南芥茎中木质素的组成。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 Epub Date: 2024-10-07 DOI: 10.1007/s10265-024-01585-6

Konan Ishida, Senri Yamamoto, Takashi Makino, Yuki Tobimatsu

{"title":"Expression of laccase and ascorbate oxidase affects lignin composition in Arabidopsis thaliana stems.","authors":"Konan Ishida, Senri Yamamoto, Takashi Makino, Yuki Tobimatsu","doi":"10.1007/s10265-024-01585-6","DOIUrl":"10.1007/s10265-024-01585-6","url":null,"abstract":"Lignin is a phenolic polymer that is a major source of biomass. Oxidative enzymes, such as laccase and peroxidase, are required for lignin polymerisation. Laccase is a member of the multicopper oxidase family and has a high amino acid sequence similarity with ascorbate oxidase. However, the process of functional differentiation between the two enzymes remains poorly understood. In this study, the common ancestry sequence of laccase and ascorbate oxidase (AncMCO) was predicted via phylogenetic reconstruction, and its in vivo effect on lignin biosynthesis in Arabidopsis thaliana was assessed. The estimated AncMCO sequence conserved key residues that coordinate with copper ions, implying that the electron transfer system is likely to be conserved in AncMCO. However, multiple insertions/deletions corresponding to protein surface structures have been found between laccase, ascorbate oxidase, and AncMCO. The overexpression of canonical laccase (AtLAC4) and ascorbate oxidase (AtAAO1) in A. thaliana resulted in notable increases of syringyl/guaiacyl lignin unit ratio in stems, whereas, in contrast, the overexpression of AncMCO did not show any detectable change in lignin deposition. Transcriptomic analysis revealed that the AtAAO1-overexpressing line exhibited significant changes in the expression of a wide range of cell wall biosynthesis genes. These results highlight the importance of the molecular evolution of multicopper oxidase, which drives lignin biosynthesis during plant evolution.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"1177-1187"},"PeriodicalIF":2.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgement. 致谢。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-11-01 DOI: 10.1007/s10265-024-01589-2

引用次数: 0