Journal of Plant Research最新文献_第2页

Identification of candidate genes for grain size in barley through combined GWAS and transcriptome analysis. 结合GWAS和转录组分析鉴定大麦籽粒大小候选基因。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-09-01 Epub Date: 2025-07-18 DOI: 10.1007/s10265-025-01657-1

Haiye Luan, Jiajia Gao, Yuhao Wu, Jinghan Yang, Yang Shen, Menglin Sun, Fangfang Liu, Meng Xu, Xiao Xu, Miao Sun, Gongneng Feng, Yinghu Zhang, Jincheng Xing

{"title":"Identification of candidate genes for grain size in barley through combined GWAS and transcriptome analysis.","authors":"Haiye Luan, Jiajia Gao, Yuhao Wu, Jinghan Yang, Yang Shen, Menglin Sun, Fangfang Liu, Meng Xu, Xiao Xu, Miao Sun, Gongneng Feng, Yinghu Zhang, Jincheng Xing","doi":"10.1007/s10265-025-01657-1","DOIUrl":"10.1007/s10265-025-01657-1","url":null,"abstract":"Barley (Hordeum vulgare L.) is an important cereal crop with diverse uses, including animal feed, beer brewing, and food production. Grain size plays a crucial role in determining grain weight and quality, which is one of the key breeding objectives in response to market and industry demands. However, the molecular mechanisms of grain size in barley are still poorly understood. In this study, 250 barley accessions were evaluated for key grain size traits, including thousand grain weight (TGW), grain length (GL), grain width (GW), grain length to width ratio (GLWR), grain area (GA) and grain perimeter (GP) across a two-year period. A total of 369 significant Single Nucleotide Polymorphisms (SNPs) significantly associated with the six grain traits were detected. Among these SNPs, 30 were continuously detected in different years. Additionally, two accessions (ZQ25 and GK5) whose grain size significantly differed were selected for transcriptome analysis. Differentially expressed genes (DEGs) were identified at two time points: 3,733 at 21 days post anthesis (DPA) and 4,396 at 28 DPA. These DEGs were enriched mainly in photosynthesis-antenna proteins, phenylpropanoid biosynthesis and flavonoid biosynthesis. Furthermore, by integrating a genome-wide association study (GWAS) and RNA-seq, we identified 25 candidate genes involved primarily in various transcription factors, phytohormones, and sugar metabolism pathways. These results provide valuable information for grain size-related gene cloning and abundant molecular data for the breeding of new high yield varieties of barley in the future.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"789-806"},"PeriodicalIF":2.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel insight into structural and functional features of the receptacle glands of Pseudobombax longiflorum (Malvaceae). 锦葵科长花假bombax longiflorum花托腺结构和功能特征的新认识。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-09-01 Epub Date: 2025-05-29 DOI: 10.1007/s10265-025-01648-2

Yve Canaveze, Elza Guimarães, Silvia Rodrigues Machado

{"title":"Novel insight into structural and functional features of the receptacle glands of Pseudobombax longiflorum (Malvaceae).","authors":"Yve Canaveze, Elza Guimarães, Silvia Rodrigues Machado","doi":"10.1007/s10265-025-01648-2","DOIUrl":"10.1007/s10265-025-01648-2","url":null,"abstract":"Floral glands, which produce diverse metabolites, have become the focus of increasing interest because of their important roles in plant-animal interactions. The glands in the receptacle of Pseudobombax longiflorum (Malvaceae) are widely accepted as nectaries. Using light, scanning, and transmission electron microscopy, we studied the anatomy, histochemistry, ultrastructure, and secretory dynamics of the receptacle glands in this species. The glands were active in secretion from young flower buds to immature fruits. Trigona spinipes was the most abundant and frequent visitor. The glands were composed of a uniseriate epidermis covered with a thick cuticle and a multilayered parenchyma, exhibiting two subregions with abundant phloem in the innermost region. These glands produced sugars, resins, oils, and phenolic compounds. Glandular cells exhibited large nuclei with nucleoli, polymorphic plastids with lipophilic inclusions, numerous mitochondria, endoplasmic reticulum profiles, cytoplasmic oil bodies, and vacuoles containing phenolic compounds. Plasmodesmata indicate that secretory components are transported from cell to cell via the symplast. There is also evidence for granulocrine and eccrine secretion mechanisms. The accumulated secretions form a bulge in the central region of the gland. The bulged epidermis detached from the parenchyma cells, and the rupture of the epidermal cells released the accumulated hydrophilic components. Residual lipophilic secretions were embedded in the epidermal cell wall and cuticle. The receptacle glands of P. longiflorum are not typical nectaries but mixed glands that produce both hydrophilic and lipophilic secretions during floral development until young fruits.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"775-787"},"PeriodicalIF":2.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and functional identification of Jasmonte resistant 1, a Jasmonic acid isoleucine-conjugating enzyme in Catharanthus roseus. 长春花茉莉酸异亮氨酸偶联酶茉莉抗性1的分离与功能鉴定。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-09-01 Epub Date: 2025-06-06 DOI: 10.1007/s10265-025-01646-4

Aikun Pan, Haiqiao Gao, Ying Lin, Bingrun Yang, Chunhao Chang, Zhiwen Liu, Yanyan Wang, Zhaoxia Jin, Fang Yu

{"title":"Isolation and functional identification of Jasmonte resistant 1, a Jasmonic acid isoleucine-conjugating enzyme in Catharanthus roseus.","authors":"Aikun Pan, Haiqiao Gao, Ying Lin, Bingrun Yang, Chunhao Chang, Zhiwen Liu, Yanyan Wang, Zhaoxia Jin, Fang Yu","doi":"10.1007/s10265-025-01646-4","DOIUrl":"10.1007/s10265-025-01646-4","url":null,"abstract":"Jasmonate resistant 1 (JAR1), which encodes a jasmonate-amino synthase, mediates the biosynthesis of jasmonoyl-isoleucine (JA-Ile), the bioactive form of jasmonate involved in JA signal transduction pathway. Although JAR1 has been characterized in several plant species, its regulatory function in mediating JA signaling to modulate the biosynthesis of terpenoid indole alkaloids (TIAs) in Catharanthus roseus, a medicinal and ornamental plant, have not been previously investigated. In this study, we have identified and cloned CrJAR1, a member of the Gretchen Hagen3 (GH3) gene family, from C. roseus and confirmed its enzymatic activity in JA-Ile formation via heterologous expression in E. coli. Functional analyses using transient overexpression and virus-induced gene silencing (VIGS) in C. roseus leaves revealed that CrJAR1 positively regulates the accumulation of catharanthine and vindoline. Moreover, we demonstrated that CrCOI1, the jasmonate receptor, functions synergistically with CrJAR1 in modulating TIA biosynthesis. Co-silencing of CrCOI1 and CrJAR1 resulted in a lower sensitivity to exogenous JA treatment compared to silencing of CrJAR1 alone, indicating distinct yet complementary roles in jasmonate-mediated metabolic regulation. These results advance our understanding the function of CrJAR1 and offer new strategies for engineering specialized metabolite biosynthesis in plants.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"825-837"},"PeriodicalIF":2.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of two G-type half-size ABC transporter genes from the lipid-secreting medicinal plant Lithospermum erythrorhizon. 产脂药用植物红紫草中两个g型半大小ABC转运蛋白基因的研究。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-09-01 Epub Date: 2025-06-16 DOI: 10.1007/s10265-025-01651-7

Takuji Ichino, Kanade Tatsumi, Yuka Munakata, Ai Tsuboyama, Eiko Moriyoshi, Masaru Nakayasu, Kojiro Takanashi, Koichiro Shimomura, Kazufumi Yazaki

引用次数: 0

Cellulose synthase machinery in thickened primary cell walls of celery collenchyma via prizm of transcriptome analysis. 纤维素合酶机制在芹菜厚壁增厚初代细胞壁中的转录组分析。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-09-01 Epub Date: 2025-07-31 DOI: 10.1007/s10265-025-01662-4

Mokshina Natalia

引用次数: 0

Have you ever seen the rain? The life cycle of Crotalaria retusa (Fabaceae) reveals reproductive resilience under drought conditions. 你见过雨吗？豆科Crotalaria retusa （Fabaceae）的生命周期揭示了干旱条件下的生殖弹性。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-09-01 Epub Date: 2025-07-18 DOI: 10.1007/s10265-025-01658-0

Cristianne Santana Santos, Bruno de Santana Santos, Marcos Vinicius Meiado

{"title":"Have you ever seen the rain? The life cycle of Crotalaria retusa (Fabaceae) reveals reproductive resilience under drought conditions.","authors":"Cristianne Santana Santos, Bruno de Santana Santos, Marcos Vinicius Meiado","doi":"10.1007/s10265-025-01658-0","DOIUrl":"10.1007/s10265-025-01658-0","url":null,"abstract":"Anthropogenic climate change is altering precipitation patterns, increasing the length of the dry season and rainfall variability. In this context, the present study aimed to evaluate the influence of drought on the life cycle and reproductive success of the invasive Crotalaria retusa L. (Fabaceae). For this, the seeds were sown in plastic pots containing 3 kg of soil from the collection area. The experiment consisted of 10 treatments with field capacity ranging from 10 to 100%. Germination traits were assessed until seedling emergence and morphological and reproductive parameters were analyzed every 10 days until the end of the reproductive phase, totaling 190 days. Reduced water availability significantly affected plant growth, reducing stem length size, stem diameter and number of leaves at the lowest field capacities evaluated. The reproductive phase was also affected by the low availability of water in the soil. Flowering had a reduction in the number of flower buds and flowers with a reduction in water content. The size and width of the fruits under 50% and 40% field capacity were smaller than in the other treatments. C. retusa is tolerant of low soil water content and can establish itself in field capacities below 30%, but has not produced flowers, fruits, and seeds. The population of the species studied shows a reduction in its growth appears to be resist severe drought and remain in the environment. This may enhance the invasive potential of the species in semi-arid environments.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"761-773"},"PeriodicalIF":2.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Local adaptation mosaic to leaf herbivores in the annual herb Datura stramonium. 一年生草本植物曼陀罗叶片食草动物的局部适应马赛克。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-08-29 DOI: 10.1007/s10265-025-01664-2

Guillermo Castillo, Adán Miranda-Pérez, Ken Oyama, Juan Núñez-Farfán

{"title":"Local adaptation mosaic to leaf herbivores in the annual herb Datura stramonium.","authors":"Guillermo Castillo, Adán Miranda-Pérez, Ken Oyama, Juan Núñez-Farfán","doi":"10.1007/s10265-025-01664-2","DOIUrl":"https://doi.org/10.1007/s10265-025-01664-2","url":null,"abstract":"Local adaptation is a central evolutionary process for creating/maintaining variation of traits mediating antagonistic interactions. However, few studies have evaluated the local adaptation of plants to their biological counterparts such as herbivores across the plants' distribution. Most studies evaluating local adaptation to herbivores have focused on specialist systems, where local adaptation is likely to occur. However, there is less evidence regarding the existence of local adaptation on generalist systems, where local adaptation is not theoretically expected. We conducted a reciprocal transplant experiment involving four local populations aimed to detect whether local adaptation in the annual herb Datura stramonium to its specialist herbivore Lema daturaphila and the generalist herbivore Sphenarium purpurascens occur. We also explored whether leaf trichome density, a putative defensive trait of D. stramonium, is mediating local adaptation to herbivores through its association with plant fitness. We found that certain D. stramonium populations were locally adapted to both herbivores but others were not, regardless of whether these are preyed upon by generalist or specialist herbivores. Leaf trichome density had a significant effect on individual fruit production, although this effect was variable across locations (origin × site interaction) and unrelated to the observed local adaptation pattern. The results support a view of a local adaptation mosaic of D. stramonium to generalist and specialist herbivores in central Mexico.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144958341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MpCAFA gene encodes a ciliary protein required for spermatozoid motility in the liverwort Marchantia polymorpha. MpCAFA基因编码一种毛毛蛋白，这种蛋白是多形地茅类精子运动所必需的。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-08-21 DOI: 10.1007/s10265-025-01666-0

Mizuki Morita, Katsuyuki T Yamato

{"title":"The MpCAFA gene encodes a ciliary protein required for spermatozoid motility in the liverwort Marchantia polymorpha.","authors":"Mizuki Morita, Katsuyuki T Yamato","doi":"10.1007/s10265-025-01666-0","DOIUrl":"https://doi.org/10.1007/s10265-025-01666-0","url":null,"abstract":"Bryophytes, pteridophytes, and some gymnosperm species produce motile ciliated spermatozoids that navigate to the egg by regulating ciliary motility in response to a concentration gradient of attractants released from the egg and/or the surrounding cells. However, the structural components of spermatozoid cilia in land plants remain largely unknown. In this study, we investigated MpCAFA (combined calcyphosine [CAPS] with flagellar-associated protein 115 [FAP115]; Mp1g04120) in the liverwort Marchantia polymorpha. The N-terminal and near C-terminal regions of MpCAFA showed similarity to CAPS, a mammalian EF-hand protein, and FAP115, a ciliary protein of the green alga Chlamydomonas reinhardtii, respectively. MpCAFA was expressed specifically in antheridia and its orthologs were found in some algae, bryophytes, pteridophytes, and some gymnosperm species, but not in most seed plants. Spermatozoids from mutants lacking functional MpCAFA exhibited a significant decrease in swimming speed. Notably, these mutants showed no obvious morphological defects, including a 9 + 2 axoneme arrangement, and retained chemotactic capability and fertility, forming normal spores. This suggests that MpCAFA is required for spermatozoid motility, but not for sperm chemotaxis or subsequent reproductive processes. The introduction of MpCAFApro:MpCAFA-mCitrine fully complemented the mutant phenotype and revealed that MpCAFA-mCitrine was localized along the lengths of the two spermatozoid cilia. Both the CAPS-like and FAP115-like domains were essential for MpCAFA function and subcellular localization in spermatozoid, whereas the C-terminal proline-rich region was required only for function. These findings indicate that MpCAFA is a major ciliary protein in land plants and can serve as a marker for visualizing spermatozoid ciliary movements.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144958416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analysis highlights the conservation significance of Torenia concolor (Linderniaceae) from the periphery of its distribution range. 基因组分析从其分布范围的外围突出了Torenia concolor （Linderniaceae）的保护意义。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-08-19 DOI: 10.1007/s10265-025-01659-z

Yuji Isagi, Taiga Shimizu, Yukihiro Kobayashi, Yoshihisa Suyama, Chinatsu Tokuhiro, Goro Kokubugata, Takuro Ito, Kuo-Fang Chung, Atsushi Abe, Takashi Makino, Michimasa Yamasaki

{"title":"Genomic analysis highlights the conservation significance of Torenia concolor (Linderniaceae) from the periphery of its distribution range.","authors":"Yuji Isagi, Taiga Shimizu, Yukihiro Kobayashi, Yoshihisa Suyama, Chinatsu Tokuhiro, Goro Kokubugata, Takuro Ito, Kuo-Fang Chung, Atsushi Abe, Takashi Makino, Michimasa Yamasaki","doi":"10.1007/s10265-025-01659-z","DOIUrl":"10.1007/s10265-025-01659-z","url":null,"abstract":"Biodiversity status assessments are typically conducted on a regional basis. Consequently, there are numerous species that are rare in one region but ubiquitously present in another country or administrative region. Correctly assessing the conservation status and value of such \"endangered species\" is essential to achieve better biodiversity conservation through the appropriate and efficient use of socioeconomic resources. A comparative genomic analysis was conducted on Torenia concolor, which is widely distributed in Southeast and East Asia, but has a limited population in Japan, specifically on Amami Oshima Island. This population has not yet been established as a conservation priority due to the possibility that it may have originated from cultivated plants. We hypothesized that the population was not due to a human-induced distribution; indeed, our findings indicate that the Amami Oshima population is derived from a natural distribution and is phylogenetically unique, retaining comparable genetic diversity with more abundant populations and exhibiting no increase in deleterious variations in their genome. These findings highlight the unique conservation significance of the Amami Oshima population. Furthermore, the findings suggest that this population, being genetically robust, may be sustainably conserved through minimal intervention strategies, such as maintaining current habitat conditions and monitoring population size, as the accumulation of deleterious mutations is comparable to that of the Taiwanese population. This study highlights the importance of accurate assessment of genomic status and contributes to a broader understanding of conservation strategies for regionally rare species.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory helix deletion in glutamate decarboxylase reduces GABA and enhances Agrobacterium-mediated transient expression in lettuce. 谷氨酸脱羧酶的调节螺旋缺失减少GABA并增强农杆菌介导的生菜瞬时表达。

IF 2.3 3区生物学

Journal of Plant Research Pub Date : 2025-08-02 DOI: 10.1007/s10265-025-01663-3

Grace Zi Hao Tan, Kanchan Sheoshankar Maurya, Shalini Krishnamoorthi, Kulaporn Boonyaves, Daisuke Urano

{"title":"Regulatory helix deletion in glutamate decarboxylase reduces GABA and enhances Agrobacterium-mediated transient expression in lettuce.","authors":"Grace Zi Hao Tan, Kanchan Sheoshankar Maurya, Shalini Krishnamoorthi, Kulaporn Boonyaves, Daisuke Urano","doi":"10.1007/s10265-025-01663-3","DOIUrl":"https://doi.org/10.1007/s10265-025-01663-3","url":null,"abstract":"Gamma-aminobutyric acid (GABA) is a metabolite involved in plant growth and stress responses, with its synthesis regulated by glutamate decarboxylase (GAD). Plant GAD enzymes have an autoinhibitory α-helix at the C-terminus, which calmodulin (CaM) binding typically relieves. Eliminating this C-terminal motif usually increases GABA levels in crops. In this case study, we generated a CRISPR/Cas9-edited lettuce line with a 14-amino acid deletion in the C-terminal helix of LsGAD2, the isozyme primarily expressed in most tissues. This targeted truncation removes CaM-binding residues while retaining the key Lys cluster (Lys489, Lys490, Lys491) responsible for autoinhibition, resulting in a significant reduction in GABA content without affecting growth. The LsGAD1/2-ΔC line showed a transcriptomic profile resembling stress responses in the wildtype under unstressed conditions. Reduced GABA levels appeared to upregulate genes involved in stress perception, signalling, and defense-related metabolic and hormonal changes, potentially mediated by WRKY-family transcription factors. Likely due to lower GABA levels and altered defense responses, LsGAD1/2-ΔC plants showed increased Agrobacterium-mediated transient expression of β-glucuronidase. Overall, our study suggests that targeted genetic manipulation of the C-terminal helix of GAD enzymes can reduce GABA levels while enhancing transformation efficiency in lettuce, thus presenting a means for engineering for such purposes.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0