Journal of Plant Research最新文献

{"title":"Allelopathic effects and phytochemical analysis of Coleanthus subtilis as possible factors involved in its colonization success.","authors":"Agnieszka Klink, Ludmiła Polechońska, Michał Gleńsk, Edyta Magdalena Gola, Zygmunt Dajdok","doi":"10.1007/s10265-026-01758-5","DOIUrl":"https://doi.org/10.1007/s10265-026-01758-5","url":null,"abstract":"<p><p>Coleanthus subtilis is a globally rare therophyte growing on exposed bottoms of water reservoirs and riverbanks. Despite its small size and ephemeral nature, it shows a potential to conquer new areas and can dominate phytocoenoses of small wetland therophytes and pioneer the land interface zones of lakes, pools and ponds. However, features enabling its spread and dominance in patches remain poorly understood. Also, the composition of biologically active compounds of the species has not been characterized in detail so far. Therefore, we assessed the species allelopathic potential as referred to the presence of biologically active compounds and ecotoxicity. Several natural products belonging to benzoxazinone derivatives, phenolic compounds, and carboxylic acids were identified in this species, which are likely to have allelopathic potential. The results of phytotoxic tests showed that water extract of C. subtilis induced a delay in germination of the model plant Sinapis alba, and, at concentrations higher than 4%, caused a significant reduction in the percentage of germinated seeds. In addition, root growth of S. alba was inhibited at concentrations of the extract greater than 4%. Oppositely, the growth of model plant hypocotyl was stimulated by low concentrations (< 4%) of the C. subtilis water extract. By showing the allelopathic potential, our results shed new light on the C. subtilis performance and absolute dominance in some patches of muddy vegetation.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149008725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trans-2-hexenal priming supports HSFA2-independent thermotolerance in Arabidopsis thaliana.","authors":"Baibhav R Barbaruah, Koso Oshima, Bhanu Rekha Vaddi, Hidetaka Ito","doi":"10.1007/s10265-026-01755-8","DOIUrl":"https://doi.org/10.1007/s10265-026-01755-8","url":null,"abstract":"<p><p>HSFA2 is a key regulator of acquired thermotolerance after mild-heat priming, but whether volatile-triggered priming depends on this pathway remains unclear. Here, we examined thermotolerance induced by the reactive green leaf volatile trans-2-hexenal (T2H) in Arabidopsis thaliana Col-0 and an hsfa2 T-DNA insertion mutant. Seedlings were primed with mild heat or a 30-min T2H pulse, recovered for 2 days, and challenged with lethal heat. T2H pretreatment improved survival in both genotypes. Survival analysis and image-based quantification of projected green area showed that T2H-supported recovery was retained in hsfa2, whereas recovery after mild-heat priming was reduced. Total chlorophyll content showed the same genotype-dependent pattern: it was lower in hsfa2 than in Col-0 after mild-heat priming but did not differ between genotypes after T2H. Early reverse-transcription quantitative PCR (RT-qPCR) analysis showed that transcript levels of HSFA1A, HSFA1B, HSFA1D, HSFA1E, and HSP101 did not correlate with the observed survival response. In a separate recovery-period analysis, HSP101 transcript abundance in hsfa2 was significantly lower than in Col-0 after both priming treatments, with the larger reduction after T2H despite strong mutant protection. RNA sequencing (RNA-seq) revealed distinct transcriptional responses between heat and volatile priming: mild heat induced broad transcriptome remodeling, whereas T2H induced a more selective stress-associated response enriched in detoxification- and redox-associated, immune-associated, and hypoxia-like transcript categories. In hsfa2, T2H was also associated with increased transcript abundance of WRKY- and MAPK-associated stress-signaling genes relative to heat priming. These findings suggest that reactive volatile priming may support thermotolerance through HSFA2-independent stress-signaling pathways.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149008731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Overexpression of the tomato SlLEA_2-26 gene enhances the tolerance to drought and salt stresses in Arabidopsis thaliana.","authors":"Zhehua Yan, Yu Lei, Xuan Zou, Sijie Wang, Yanxin Yang, Dongjing Yang, Lingzhi Li, Wenbin Wang","doi":"10.1007/s10265-026-01750-z","DOIUrl":"https://doi.org/10.1007/s10265-026-01750-z","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Exploring genetic diversity and selective signatures, a journey through Colombian cassava's landscape.","authors":"Rubén Eduardo Mora Moreno, Juan Sebastián Sánchez Ferro, Emili Ishikawa Garcia, Andrés David Jiménez Maldonado, Paula Alejandra Diaz Tatis, Camilo Ernesto López Carrascal, Johana Carolina Soto Sedano","doi":"10.1007/s10265-026-01720-5","DOIUrl":"https://doi.org/10.1007/s10265-026-01720-5","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AspAT orchestrates metabolic and transcriptional networks for drought adaptation in poplar.","authors":"Mei Han, Yujia Si, Shuang Liu, Yuqi Zhu, Yizhen Sun, Tao Su","doi":"10.1007/s10265-026-01753-w","DOIUrl":"https://doi.org/10.1007/s10265-026-01753-w","url":null,"abstract":"<p><p>Escalating climate-induced drought events are increasingly pushing terrestrial plants toward their physiological limits, challenging their growth and ecological acclimation. Metabolic reprogramming represents a critical adaptive strategy under water deficit, in which aspartate aminotransferase (AspAT) serves as a key node by modulating carbon-nitrogen flux, energy metabolism, and amino acid biosynthesis. Although AspAT genes have been implicated in abiotic stress responses in herbaceous species, their roles in woody plants remain poorly characterized. Here, we identified multiple drought-responsive AspAT family members in poplar and further characterized the function of AspAT10. Transgenic poplars overexpressing AspAT10 (OE) exhibited higher drought tolerance than wild-type (WT) controls, maintaining higher growth rates, greater biomass accumulation, and improved leaf water status under drought conditions. Consistent with these phenotypes, OE lines displayed elevated relative water content, photosynthetic performance, and lignin accumulation relative to WT plants. Transcriptome analysis revealed that AspAT10 overexpression was associated with the upregulation of genes involved in amino acid metabolism, as well as pathways related to cuticular wax formation. These findings suggest that AspAT10 may contribute to drought responses through coordinated regulation of metabolic and transcriptional processes, thereby supporting plant growth under drought stress.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional analysis of MeJA-responsive bHLH family genes in Taraxacum kok-saghyz.","authors":"Fengqi Luo, Changping Zhang, Jialong Sun, Zihan Guo, Yuya Geng, Xifeng Chen, Jie Yan","doi":"10.1007/s10265-026-01751-y","DOIUrl":"https://doi.org/10.1007/s10265-026-01751-y","url":null,"abstract":"<p><p>Taraxacum kok-saghyz (T. kok-saghyz) is considered a highly promising alternative source of natural rubber (NR), as its roots synthesize high-molecular-weight NR comparable to that produced by Hevea brasiliensis. The basic helix-loop-helix (bHLH) family of transcription factors (TFs) plays crucial roles in plant organogenesis, hormonal signal transduction, and the regulation of secondary metabolism. This study aimed to systematically identify TkbHLH family members and to elucidate their potential functions in responding to methyl jasmonate (MeJA) and regulating root development. Based on the T. kok-saghyz genome, 172 TkbHLH members were identified and phylogenetically classified into 16 subfamilies. Among these, 37 genes were selected due to their significant induction by MeJA. Sequence analysis confirmed all encoded proteins contain the conserved bHLH domain. Subcellular localization verified nuclear localization of five core TkbHLH proteins. Interactions were shown by yeast two-hybrid and bimolecular fluorescence complementation, revealing these proteins form homodimers and heterodimers. Notably, a specific interaction was detected between TkbHLH162 and TkHMGS1, a key enzyme in the mevalonate (MVA) pathway, suggesting a potential molecular link between JA signaling and the rubber biosynthesis precursor pathway. Functional characterization via overexpression assays showed that selected TkbHLH genes significantly either promoted or inhibited root elongation. In summary, this study presents the first systematic characterization of the bHLH TF family in T. kok-saghyz, elucidating its involvement in JA signal response, protein interaction networks, and root development regulation. These findings provide a crucial foundation for further investigation into the molecular mechanisms by which TkbHLH TFs influence root morphogenesis and NR biosynthesis in T. kok-saghyz.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny and taxonomic revisions of Piper (Piperaceae) in Taiwan.","authors":"Po-Hao Chen, Chia-Hao Chang, Kuan-Ning Kung, Hung-Chih Lin, An-Ching Chung, Chia-Ying Ou, Shih-Hui Liu","doi":"10.1007/s10265-026-01733-0","DOIUrl":"10.1007/s10265-026-01733-0","url":null,"abstract":"<p><p>Taiwan, located at the northernmost extent of Huxley's Line, marks the furthest point along this biogeographic boundary where exchanges of flora and fauna occur. The genus Piper, a globally distributed and taxonomically complex group of both economic and evolutionary importance, has a convoluted taxonomic history in this transitional zone that requires comprehensive study. Here, we integrate morphological, molecular, and distributional data to revise the taxonomy of Taiwanese Piper, resolve their evolutionary relationships, and infer their biogeographic history. Our revision recognizes 11 species in Taiwan, including seven native taxa, of which three are endemic. The present analysis establishes the first comprehensive phylogenetic framework for Taiwanese Piper and reveals that native species arose from lineages on both sides of Wallace's Line. Collectively, these findings provide the first evidence of bidirectional expansion within a genus in Taiwan and position Piper as an exemplary system for examining phylogeographic dynamics in transitional biogeographic zones.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"685-716"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LRS1 phosphorylated in response to blue light regulates stomatal opening.","authors":"Kohei Fukatsu, Yuki Hayashi, Keiko Kuwata, Toshinori Kinoshita","doi":"10.1007/s10265-026-01747-8","DOIUrl":"10.1007/s10265-026-01747-8","url":null,"abstract":"<p><p>Blue light receptor phototropin is a protein kinase that initiates various blue light signaling pathways, including the phosphorylation and activation of plasma membrane (PM) H⁺-ATPase for stomatal opening. Activation of PM H⁺-ATPase drives K⁺ uptake via K⁺_in channels. This influx of K⁺ is electrically balanced by intracellular anions such as Cl⁻ and malate^2-, increasing cellular osmotic potential and driving water influx and stomatal opening. Hypothesizing that unidentified phosphorylation events regulate this process, we performed phosphoproteomic analysis to elucidate the link between blue light signaling and stomatal opening. We found that blue light induces phosphorylation of Ser395 in Vicia faba LATERAL ROOT STIMULATOR 1 (VfLRS1) in guard cell protoplasts. VfLRS1 shows high similarity to Arabidopsis thaliana LATERAL ROOT STIMULATOR 1 (LRS1, AT3G05090), and the phosphorylation site is conserved as Ser393 in A. thaliana LRS1. Therefore, we investigated stomatal phenotype using a T-DNA-inserted knockout mutant of A. thaliana LRS1, lrs1. The lrs1 mutant exhibited normal blue light-induced stomatal opening and normal blue light-induced phosphorylation of Thr948 of PM H⁺-ATPase (numbering according to AHA1) in guard cells. The lrs1 mutant showed reduced starch accumulation during dark and red light illumination. Blue light-induced starch degradation was also severely suppressed in the lrs1 mutant. The reduced starch accumulation in lrs1 guard cells may have been associated with reduced levels of starch-derived metabolites, such as malate. We then examined blue light-induced stomatal opening in potassium gluconate (KGlu) buffer, in which most Cl^- was replaced by the poorly membrane-permeable gluconate anion. As expected, the lrs1 mutant showed significantly reduced blue light-induced stomatal opening under this condition. Taken together, our results indicate that LRS1 plays important roles in starch dynamics and contributes to stomatal opening.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"911-919"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Origin of the Eleusine indica population on the active volcanic Nishinoshima Island, Japan.","authors":"Koutaro Maeda, Takaki Aihara, Kentaro Uchiyama, Mengying Cai, Widiyatno, Takashi Kamijo, Yoshihiko Tsumura","doi":"10.1007/s10265-026-01738-9","DOIUrl":"10.1007/s10265-026-01738-9","url":null,"abstract":"<p><p>Nishinoshima Island, located approximately 1,000 km south of mainland Japan and 130 km from the Ogasawara Islands, emerged as a new island due to volcanic activity in 1973. On this isolated island, grasses such as Eleusine indica and Portulaca oleracea have been observed growing, though their routes of invasion remain unknown. Numerous seabirds have been spotted inhabiting and breeding around Nishinoshima Island, potentially contributing to the spread of these plants without any possibility of anthropogenic influence. The aim of this study was to trace the origin of the E. indica population on Nishinoshima Island using genetic methods. We collected samples of E. indica from Nishinoshima Island, mainland Japan, the Ryukyu Islands, the Ogasawara Islands, and other locations. In this study, we utilized two types of DNA with different inheritance modes: chloroplast DNA (cpDNA) and nuclear DNA. For cpDNA, five polymorphic regions were analyzed, while genetic diversity and structure were also assessed for nuclear DNA using MIG-seq (Multiplexed inter-simple sequence repeat (ISSR) genotyping by sequencing), a method for detecting nucleotide polymorphisms with next-generation sequencing technology. Genetic diversity analysis revealed high genetic diversity in the mainland Japan population. Additionally, cpDNA analysis confirmed the presence of the Nishinoshima haplotype on several islands, including Ogasawara Island. Nuclear DNA analysis showed a close relationship between the Nishinoshima population and those on Ogasawara, Hachijojima, Okinawa and Indonesia. Given instances of seabirds carrying tiny seeds over long distances, it is plausible that E. indica seeds were transported from distant locations by migrating birds. Based on the genetic diversity of E. indica on Nishinoshima, it was considered that this population originated from a single or a few seed as a result of an extremely rare seed dispersal event.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"717-730"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148684670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reconstructing the survival history of relict herbs endemic to the heavy snowfall regions of Japan during the Pleistocene.","authors":"Watanabe Yoichi, Momoka Gozono, Hinata Koide, Hotaka Ishida, Koki Nagasawa, Kazutoshi Masuda, Shota Sakaguchi","doi":"10.1007/s10265-026-01727-y","DOIUrl":"10.1007/s10265-026-01727-y","url":null,"abstract":"<p><p>The effects of past environmental changes on the demography and genetic diversity of relict species remain poorly understood. Such studies have rarely been conducted across multiple phylogenetically distant species, largely because of their rarity. Here, we investigated impacts of long-term climatic changes on the evolution and survival of three herbaceous Tertiary relict species endemic to heavy snowfall regions of Japan. Using population genomic analyses based on plastome sequences, we reconstructed phylogeographic patterns, including demographic histories, and explored potential drivers of similarities and differences among the species. Divergence times from close relatives were estimated at ca. 64 Ma for Glaucidium palmatum, 23 Ma for Ranzania japonica, and 12 Ma for Paris japonica, all of which predate the Pleistocene. The time to the most recent common ancestor (tMRCA) for each species ranged from approximately 0.03 to 3 Ma. Effective population sizes of species and regional groups expanded rapidly between approximately 100,000 and 10,000 years before present. Patterns of genetic diversity and geographic structure among the three relict species appear to reflect differences in dispersal ability and the locations of climatic refugia. Despite variation in tMRCAs among the three species, demographic expansions consistently occurred during the late Pleistocene and were likely associated with the development of heavy snowfall climate.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"731-743"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148295552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}