Journal of Plant Research最新文献

{"title":"Insights into chloroplast genome structure and phylogenetic position of the Lacquer tree Toxicodendron trichocarpum (Anacardiaceae: Rhoideae).","authors":"Yujie Xu, Liya Niu, M James C Crabbe, Xu Su, Zhumei Ren","doi":"10.1007/s10265-025-01661-5","DOIUrl":"https://doi.org/10.1007/s10265-025-01661-5","url":null,"abstract":"<p><p>The lacquer tree Toxicodendron trichocarpum (Miq) Kuntze (Anacardiaceae: Rhoideae), which is known for its abundance of urushiol, produces a high-quality raw lacquer. There have been five complete chloroplast genomes reported in the genus Toxicodendron, while, only two T. trichocarpum genes were sequenced and used for phylogenetic and biogeographic analysis. In this study, we sequenced the T. trichocarpum whole genome on the Illumina HiSeq 4000 platform and investigated its complete chloroplast genome characteristics. Our results showed that the length of the chloroplast genome of T. trichocarpum was 159,959 bp with a GC content of 37.9%. The genome was found to comprise a large single-copy region (LSC) spanning 87,964 bp, a small single-copy region (SSC) of 18,979 bp, and two inverted repeat regions (IRa/IRb) of equal length, each measuring 26,508 bp. A total of 134 genes were annotated, consisting of 89 protein-coding genes, 37 tRNA genes and eight rRNA genes. Altogether, 84 chloroplast simple sequence repeats (SSRs) were identified, predominantly composed of single nucleotides (A/T), with a clear preference for A/T. By comparing the chloroplast genomes of the genus Toxicodendron, six relatively highly variable regions were identified for future molecular marker development. Phylogenetic analyses conducted within the tribe Rhoideae strongly supported the monophyly of the genus Toxicodendron. The species T. trichocarpum was sister to T. diversilobum, with a high level of support at 99%, forming a clade with T. griffithii and T. vernicifluum. Divergence time estimation suggested that Toxicodendron was originated at 42.47 mya (95% HPD: 41.99-42.99 mya) and T. trichocarpum occurred concomitantly with the sister species T. diversilobum at approximately 16.33 mya. We first reported the complete chloroplast genome of T. trichocarpum and comparative analysis of the Toxicodendron species, which will provide valuable genomic information for accurate classification and evolutionary process of this important taxa in the future.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of candidate genes for grain size in barley through combined GWAS and transcriptome analysis.","authors":"Haiye Luan, Jiajia Gao, Yuhao Wu, Jinghan Yang, Yang Shen, Menglin Sun, Fangfang Liu, Meng Xu, Xiao Xu, Miao Sun, Gongneng Feng, Yinghu Zhang, Jincheng Xing","doi":"10.1007/s10265-025-01657-1","DOIUrl":"https://doi.org/10.1007/s10265-025-01657-1","url":null,"abstract":"<p><p>Barley (Hordeum vulgare L.) is an important cereal crop with diverse uses, including animal feed, beer brewing, and food production. Grain size plays a crucial role in determining grain weight and quality, which is one of the key breeding objectives in response to market and industry demands. However, the molecular mechanisms of grain size in barley are still poorly understood. In this study, 250 barley accessions were evaluated for key grain size traits, including thousand grain weight (TGW), grain length (GL), grain width (GW), grain length to width ratio (GLWR), grain area (GA) and grain perimeter (GP) across a two-year period. A total of 369 significant Single Nucleotide Polymorphisms (SNPs) significantly associated with the six grain traits were detected. Among these SNPs, 30 were continuously detected in different years. Additionally, two accessions (ZQ25 and GK5) whose grain size significantly differed were selected for transcriptome analysis. Differentially expressed genes (DEGs) were identified at two time points: 3,733 at 21 days post anthesis (DPA) and 4,396 at 28 DPA. These DEGs were enriched mainly in photosynthesis-antenna proteins, phenylpropanoid biosynthesis and flavonoid biosynthesis. Furthermore, by integrating a genome-wide association study (GWAS) and RNA-seq, we identified 25 candidate genes involved primarily in various transcription factors, phytohormones, and sugar metabolism pathways. These results provide valuable information for grain size-related gene cloning and abundant molecular data for the breeding of new high yield varieties of barley in the future.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Have you ever seen the rain? The life cycle of Crotalaria retusa (Fabaceae) reveals reproductive resilience under drought conditions.","authors":"Cristianne Santana Santos, Bruno de Santana Santos, Marcos Vinicius Meiado","doi":"10.1007/s10265-025-01658-0","DOIUrl":"https://doi.org/10.1007/s10265-025-01658-0","url":null,"abstract":"<p><p>Anthropogenic climate change is altering precipitation patterns, increasing the length of the dry season and rainfall variability. In this context, the present study aimed to evaluate the influence of drought on the life cycle and reproductive success of the invasive Crotalaria retusa L. (Fabaceae). For this, the seeds were sown in plastic pots containing 3 kg of soil from the collection area. The experiment consisted of 10 treatments with field capacity ranging from 10 to 100%. Germination traits were assessed until seedling emergence and morphological and reproductive parameters were analyzed every 10 days until the end of the reproductive phase, totaling 190 days. Reduced water availability significantly affected plant growth, reducing stem length size, stem diameter and number of leaves at the lowest field capacities evaluated. The reproductive phase was also affected by the low availability of water in the soil. Flowering had a reduction in the number of flower buds and flowers with a reduction in water content. The size and width of the fruits under 50% and 40% field capacity were smaller than in the other treatments. C. retusa is tolerant of low soil water content and can establish itself in field capacities below 30%, but has not produced flowers, fruits, and seeds. The population of the species studied shows a reduction in its growth appears to be resist severe drought and remain in the environment. This may enhance the invasive potential of the species in semi-arid environments.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A natural 294-bp indel in the VviHKT1;1 proximal promoter influences the salt resistance of VviHKT1;1-overexpressing Arabidopsis.","authors":"Fengjie Wang, Xiaoying Gong, Xuenan Zhang, Rong Zhao, Ning Han, Zhen Yang","doi":"10.1007/s10265-025-01656-2","DOIUrl":"https://doi.org/10.1007/s10265-025-01656-2","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of water availability on floral traits and pollinators in a columnar cactus endemic to the Atacama Desert, Chile.","authors":"Daniel Piñones-Tapia, Patricia C Guerra, Antoine Touret, Gerardo O Gutiérrez, Jaime Pizarro-Araya, Ernesto Gianoli","doi":"10.1007/s10265-025-01655-3","DOIUrl":"https://doi.org/10.1007/s10265-025-01655-3","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of single nucleus RNA-seq and bulk RNA-seq reveals gene regulatory networks for vascular connection between parasitic plants and host plants.","authors":"Mengqi Cui, Dongbo Shi, Momoko Yamaji, Kie Kumaishi, Yasunori Ichihashi, Ayako Kawamura, Keiko Sugimoto, Satoko Yoshida","doi":"10.1007/s10265-025-01654-4","DOIUrl":"https://doi.org/10.1007/s10265-025-01654-4","url":null,"abstract":"<p><p>The facultative parasitic plant Phtheirospermum japonicum forms a specialized organ, the haustorium, to invade its host, Arabidopsis thaliana, establishing a vascular connection via the formation of a xylem bridge. This connection depends on coordinated interactions between the vascular systems of both plants, yet the molecular dynamics of these interactions within the haustorium and the host roots remain elusive. This study aimed to unravel the transcriptomic heterogeneity of haustoria and gene regulatory networks involved in this process by integrating single nucleus RNA sequencing (snRNA-seq) and bulk RNA sequencing (bulk RNA-seq). snRNA-seq identified a total of 7 P. japonicum cell clusters and 4 A. thaliana cell clusters, each with cluster-specific marker genes, allowing for a distinct characterization of vascular cells within the haustorium. Differential gene expression analyses revealed up-regulation of genes associated with xylem formation and auxin transport in both parasites and hosts, suggesting the presence of shared molecular mechanisms facilitating vascular connection. Further gene network analysis combining snRNA-seq and bulk RNA-seq identified conserved homologous genes across both species, indicating potential molecular interactions of vascular-related genes from hosts and parasites. Our study reveals the high heterogeneity of haustorium cells, characterizing the expression profiles of each cell type in haustoria and host roots during haustorium development at single-cell resolution. These findings provide insights into the molecular interactions between parasitic plants and hosts, presenting potential targets for disrupting these interactions to manage parasitic plant infestations in crops.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Over-compensation of the native herb Qiai (Artemisia argyi var. argyi cv. Qiai) to infection with alien field dodder (Cuscuta campestris).","authors":"Xin Yu, Tong Wu, Wei-Bin Wang, Tian-Yu Ma, Qiu-Yu Ma, Jia-Ying Zhang, Jia-Liang Zhang","doi":"10.1007/s10265-025-01639-3","DOIUrl":"10.1007/s10265-025-01639-3","url":null,"abstract":"<p><p>Compensatory growth of crops is argued to alleviate detrimental parasitic effects. Cuscuta campestris causes severe crop losses worldwide, and is the most important parasitic weed. In a two-year consecutive field investigation, C. campestris was found on Artemisia argyi var. argyi cv. Qiai, one traditional medicinal herb with a long cultivation history in Qizhou, China. In 2020, anatomical validation was conducted on haustorial connection, and competition between carbon (C) and nitrogen (N) in this parasitic system was quantified with isotope signature (δ¹³C, δ¹⁵N) at five sites in 2021. Across sites, leaf senescence of low canopy of infected Qiai was greatly accelerated. Enhancement of resource remobilization from aging leaves (emerging source) mainly supported growth of the parasite (additional sink) also host stem, Qiai successfully abstained intense resource competition with C. campetris, and both species had similar δ¹³C and δ¹⁵N. A new source-sink balance re-established between the parasite and host. Infected Qiai held relatively steady canopy C fixation (constant photosynthetic N use efficiency, biomass and N concentration of green leaves), and branched in top canopy markedly, increasing light access. Together, Qiai appeared to enhance canopy C gain in response to infection. Increase in δ¹³C in this parasite-host association was mild. Finally, this parasite evidently over-stimulated the aboveground biomass of its host, irrespective of infection density and load, and was left behind. This study first reported C. campestris on Qiai, and the latter species execute over-compensation in the field.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"575-586"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bud structure, time of budbreak and crown architecture in woody species from Cerrado and seasonal forests of Brazil.","authors":"Gabriel Tadeu Teodoro da Cruz, Gabriela Brito Costa, Nayara Magry Jesus Melo, Javier Guido Puntieri, João Paulo Souza","doi":"10.1007/s10265-025-01642-8","DOIUrl":"10.1007/s10265-025-01642-8","url":null,"abstract":"<p><p>Different light intensities in cerrado stricto sensu (CSS) and semideciduous seasonal forests (SSF) can result in distinct morphological responses among woody species. This research evaluated the size and bud composition, budbreak time, and crown architecture of woody species in response to precipitation and varying light intensities in these two environments. The study was conducted in CSS (19°57'29″ S and 44°25'29″ W) and an SSF fragment (19°53'84″ S and 44°25'56″ W) in Minas Gerais, Brazil. The research focused on four species: Miconia albicans and Xylopia aromatica, which occur in both environments, as well as Bauhinia cfr. ungulata (CSS) and B. cfr. rufa (SSF). Shoots from the main axis were manually dissected, and budbreak times were recorded. Crown architecture was evaluated based on diagrams of the vegetative above-ground structure, excluding leaves. Light intensities was measured with a luxmeter at the crown's apex, interior, and base. Shoots were larger and had more preformed organs in CSS than in SSF trees. Trichomes were observed on shoots of all CSS and SSF trees. The number of cataphylls varied: B. cfr. ungulata had one, B. cfr. rufa had 1-3, while compound buds of X. aromatica averaged 5.4 in CSS and 3.7 in SSF. Simple buds of X. aromatica and all M. albicans buds lacked cataphylls. Budbreak occurred in September for M. albicans (CSS and SSF), October for B. cfr. ungulata, Juy-October for X. aromatica and August-October for B. cfr. rufa. A positive correlation between budbreak and rainfall was recorded only for B. cfr. ungulata. Despite differences in bud size and composition between environment, these didn't result in distinct crown architectures. The findings highlight that tropical woody species with cataphyll-protected buds are as common as in temperate regions. Further research is needed to explore phylogenetic traits and the ecological role of cataphylls in tropical species.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"587-601"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abscisic acid enhances non-photochemical quenching through SnRK2 and ABI3 in Physcomitrium patens.","authors":"Chang-Hyun Maeng, Takuya Fujita, Junko Kishimoto, Ryouichi Tanaka, Atsushi Takabayashi, Tomomichi Fujita","doi":"10.1007/s10265-025-01627-7","DOIUrl":"10.1007/s10265-025-01627-7","url":null,"abstract":"<p><p>The transition of plants in the green lineage from aquatic to terrestrial environments during the bryophyte stage marked a pivotal point in evolution. Successful terrestrialization required evolutionary adaptations to harsh and fluctuating light conditions, where direct irradiation is stronger than in aquatic environments. To cope with these challenges, plants evolved regulatory mechanisms to control cellular activities. One such acclimation is rapidly reversible, energy-dependent non-photochemical quenching (NPQ), which dissipates excess light energy as heat to protect the photosynthetic apparatus. Another critical innovation is abscisic acid (ABA) signaling, believed to have first emerged in bryophytes. Here, we reveal a potential link between these two key acclimations in bryophytes. We demonstrate that exogenous ABA induces NPQ in the moss Physcomitrium patens, increasing the levels of LHCSR, a key NPQ regulator, while concurrently decreasing PsbS. Exogenous ABA also enhances the xanthophyll cycle pigments, contributing to NPQ. In mutants deficient in ABA signaling components, including SNF1-related kinase 2 (SnRK2) and the transcription factor, Abscisic Acid-Insensitive 3 (ABI3), ABA-induced NPQ, LHCSR and PsbS expression, and xanthophyll cycle pigment accumulation were significantly reduced. These findings suggest that exogenous ABA enhances NPQ through the SnRK2 and ABI3-mediated signaling pathway by promoting LHCSR expression and xanthophyll cycle pigment production. We propose that the integration of ABA signaling and NPQ represent a critical evolutionary milestone, enabling early land plants to adapt and thrive in terrestrial environments.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"625-636"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetracentron (Trochodendraceae) in the Paleocene and Miocene of western North America.","authors":"Steven R Manchester","doi":"10.1007/s10265-025-01636-6","DOIUrl":"10.1007/s10265-025-01636-6","url":null,"abstract":"<p><p>Although Tetracentron (Trochodendraceae) is endemic to eastern Asia today, the fossil record indicates that it was formerly more widespread across the northern hemisphere. Infructescences from the Paleocene of Wyoming, USA, documented herein, represent the oldest known occurrence of the genus. Details of the morphology, including spikes of sessile, tetracarpellate, apically dehiscent capsules with styles emerging from the lower part of the fruit, and a nectary bulge beneath each style, are revealed by micro-CT scanning of specimens preserved as molds and casts in siltstone. The discovery of Tetracentron linchensis sp. nov. indicates that Tetracentron and Trochodendron had already diverged by about 60 million years ago and were sympatric, along with the extinct relative, Eotrochion, in the Paleocene of Wyoming. North American fossil occurrences of Trochodendraceae, including extinct Eocene and Miocene genera, as well as both extant genera, highlight a former diversity and geographic spread that is no longer evident in the surviving Asian Trochodendraceae.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"555-561"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}