Journal of Plant Research最新文献

Application of ultra-weak photon emission imaging in plant stress assessment.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-01-05 DOI: 10.1007/s10265-024-01600-w

Ankush Prasad, Eliška Mihačová, Renuka Ramalingam Manoharan, Pavel Pospíšil

引用次数: 0

New Year's greetings 2025 from the Journal of Plant Research.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-01-02 DOI: 10.1007/s10265-024-01605-5

Maki Katsuhara

引用次数: 0

Synergistic effect of alkane and membrane lipid alteration in Synechococcus elongatus PCC 7942 under salt and light stresses.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-31 DOI: 10.1007/s10265-024-01613-5

Arif Agung Wibowo, Koichiro Awai

{"title":"Synergistic effect of alkane and membrane lipid alteration in Synechococcus elongatus PCC 7942 under salt and light stresses.","authors":"Arif Agung Wibowo, Koichiro Awai","doi":"10.1007/s10265-024-01613-5","DOIUrl":"https://doi.org/10.1007/s10265-024-01613-5","url":null,"abstract":"Salinity and light markedly influence cyanobacterial viability. High salinity disrupts the osmotic balance, while excess light energy affects redox potential in the cells. Regulating the ratio of saturated and unsaturated alka(e)ne and fatty acids in cyanobacteria is thought to have crucial roles in coping with these stresses by regulating membrane fluidity. In Synechococcus elongatus PCC 7942 (Syn7942), alkane is produced from fatty acid metabolites using acyl-acyl carrier protein reductase (Aar) and aldehyde-deformylating oxygenase (Ado) enzymes. However, the role of alka(e)nes and their correlation with fatty acid-related compounds, especially under salinity stress, is not yet fully understood. This study explored the significance of the natural alka(e)ne biosynthesis pathway using Syn7942. The role of alka(e)ne was assessed using single and double knockout mutants of the aar and/or ado genes in this biosynthetic process. The alka(e)ne levels and membrane lipid content exhibited an inverse relationship, correlating with cell fluidity under high-salinity and high-light conditions. The absence of alka(e)ne resulted in a severe growth phenotype of Δado and Δaar/Δado under high-salinity conditions and less severe under high-light conditions. In addition, feeding with C15:0 and/or C17:0 alkanes complemented the growth phenotype with different accumulation profiles. The Δaar mutant exhibited higher resistance to high salinity than the Syn7942 WT, indicating the importance of Ado for survival at high salinity. Overall, lipid-related compounds, especially alka(e)nes, markedly contribute to cell integrity maintenance under high-salinity conditions by regulating membrane rigidity and fluidity.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a novel photoperiod-insensitive allele Ppd-B1a.3 and its effect on heading date in Chinese wheat (Triticum aestivum) cultivar Qingchun 37.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-31 DOI: 10.1007/s10265-024-01609-1

Tianqi Song, Caiyin Shi, Yukun Wang, Sihai Guo, Weijun Zhang, Xiaoxing Wang, Jianfei Zhou, Yaning Bu, Siyi Li, Qiru Fan, Fan Wei, Jishan Xiang, Dongsheng Chen, Xiaoke Zhang

{"title":"Molecular characterization of a novel photoperiod-insensitive allele Ppd-B1a.3 and its effect on heading date in Chinese wheat (Triticum aestivum) cultivar Qingchun 37.","authors":"Tianqi Song, Caiyin Shi, Yukun Wang, Sihai Guo, Weijun Zhang, Xiaoxing Wang, Jianfei Zhou, Yaning Bu, Siyi Li, Qiru Fan, Fan Wei, Jishan Xiang, Dongsheng Chen, Xiaoke Zhang","doi":"10.1007/s10265-024-01609-1","DOIUrl":"https://doi.org/10.1007/s10265-024-01609-1","url":null,"abstract":"Breeders adjust wheat heading dates to improve regional adaptability and reduce or mitigate yield losses caused by meteorological disasters, pests and diseases. The Ppd-1 genes play a crucial role in determining wheat sensitivity to changes in day-length and serve as key regulators of heading dates once the vernalization requirement is satisfied. In this study, we identified a new allelic variant of the promoter region, Ppd-B1a.3, in the Chinese wheat cultivar Qingchun 37. Compared to the Ppd-B1b.1 (carried by Chihokukomugi), the main mutation sites in Ppd-B1a.3 include a substitution of C with G at the -505-bp, a T base insertion at the -625-bp, a mutation of TCG to GGT at the -632 to -634-bp, and a 163-bp insertion at the -691 bp. Analysis of F2 populations indicated that Ppd-B1a.3 promotes heading and flowering (approximately 6 days earlier in population 1 and 17 days in population 2) under short-day conditions in a greenhouse. However, the evaluation of Ppd-B1a.3's effect under field conditions may be influenced by the copy number of the Ppd-B1 locus inherited from the other parent in the F2 populations. Ppd-B1a.3 disrupts circadian rhythm expression and exhibits a stronger effect on heading and flowering than the three-copy Ppd-B1 allele carried by Jing 411. Origin analysis suggests that Ppd-B1a.3 may have derived from non-native germplasm. These results deepen our understanding of wheat photoperiod genes and provide useful genetic resources for fine-tuning wheat heading dates during breeding.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Floral developmental insights into two species of Erythrina (Fabaceae: Papilionoideae: Phaseoleae) pollinated by hummingbirds and passerines.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-27 DOI: 10.1007/s10265-024-01610-8

Lukas Gabriel Macedo Pessanha de Souza, Marcus José de Azevedo Falcão, João Paulo Basso-Alves, Vidal de Freitas Mansano

{"title":"Floral developmental insights into two species of Erythrina (Fabaceae: Papilionoideae: Phaseoleae) pollinated by hummingbirds and passerines.","authors":"Lukas Gabriel Macedo Pessanha de Souza, Marcus José de Azevedo Falcão, João Paulo Basso-Alves, Vidal de Freitas Mansano","doi":"10.1007/s10265-024-01610-8","DOIUrl":"https://doi.org/10.1007/s10265-024-01610-8","url":null,"abstract":"Erythrina is a Pantropical bird-pollinated genus of Fabaceae. Thus, its flowers are usually large, showy, red or yellowish, offering nectar as the principal resource. There are two main interaction systems with birds in Erythrina: in one, the inflorescences are erect and the flowers are horizontal, offering no landing platform; in the other, the inflorescences are horizontal and the flower parts are more exposed. Erythrina speciosa is pollinated by hummingbirds and E. poeppigiana is pollinated by passerines. Despite their structural variation, little is known about how species of the same genus diverge ontogenetically to form flowers adapted to pollinators with different beak morphology and feeding behaviors. Therefore, this study aimed to investigate floral development in two species according to their pollination system. Flowers and buds were collected and fixed for analysis using scanning electron microscopy and light microscopy. Some characteristics are common to both species: the formation of a pseudoracemose inflorescence, the unidirectional emergence of floral organs, and the formation of a short staminal sheath involving nine of the ten stamens (diadelphous androecium). Other characteristics, notably those related to the late stages of floral development, gradually diverged. Among them are inflorescence formation pattern; the formation of reduced and free keel petals in E. speciosa, while in E. poeppigiana they are longer and postgenitally united by their lower margins; and the participation of the standard in the floral display. The studied species share several traits common to other Papilionoideae, but some similarities between the species studied may not be phylogenetically related and reveal the potential ontogenetic pathways of functional convergence that flowers have experienced throughout evolution in the genus.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chlorophyll fluorescence responses to CO₂ availability reveal crassulacean acid metabolism in epiphytic orchids.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-24 DOI: 10.1007/s10265-024-01608-2

Sae Bekki, Kenji Suetsugu, Koichi Kobayashi

{"title":"Chlorophyll fluorescence responses to CO2 availability reveal crassulacean acid metabolism in epiphytic orchids.","authors":"Sae Bekki, Kenji Suetsugu, Koichi Kobayashi","doi":"10.1007/s10265-024-01608-2","DOIUrl":"https://doi.org/10.1007/s10265-024-01608-2","url":null,"abstract":"Crassulacean acid metabolism (CAM), a specialized mode of photosynthetic carbon assimilation characterized by nocturnal fixation of atmospheric CO2 and vacuolar malic acid storage, is found in a wide variety of vascular plant species, mainly those inhabiting water-limited environments. Identifying and characterizing diverse CAM species enhances our understanding of the physiological, ecological, and evolutionary significance of CAM photosynthesis. In this study, we examined the effect of CO2 elimination on chlorophyll fluorescence-based photosynthetic parameters in two constitutive CAM Kalanchoe species and six orchids. In CAM-performing Kalanchoe species, the effective quantum yield of photosystem II showed no change in response to CO2 elimination during the daytime but decreased with CO2 elimination at dusk. We applied this method to reveal the photosynthetic mode of epiphytic orchids and found that Gastrochilus japonicus, Oberonia japonica, and Bulbophyllum inconspicuum, but not B. drymoglossum, are constitutive CAM plants, which were also confirmed by malate determination. Our data propose a novel approach to identify and characterize CAM plants without labor-intensive experimental procedures. Although B. drymoglossum leaves had relatively high malate content, they did not depend on it to perform photosynthesis even under water-deficient or increased light conditions. Anatomical comparisons revealed a notable difference in leaf structure between B. drymoglossum and B. inconspicuum; B. drymoglossum leaves possess large water storage tissue internally, unlike B. inconspicuum leaves, which develop pseudobulbs. Our findings suggest different evolutionary adaptations to water deficit between closely related B. drymoglossum and B. inconspicuum.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plastidial thioredoxin-like proteins are essential for normal embryogenesis and seed development in Arabidopsis thaliana.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-21 DOI: 10.1007/s10265-024-01611-7

Yuka Fukushi, Yuichi Yokochi, Toru Hisabori, Keisuke Yoshida

{"title":"Plastidial thioredoxin-like proteins are essential for normal embryogenesis and seed development in Arabidopsis thaliana.","authors":"Yuka Fukushi, Yuichi Yokochi, Toru Hisabori, Keisuke Yoshida","doi":"10.1007/s10265-024-01611-7","DOIUrl":"https://doi.org/10.1007/s10265-024-01611-7","url":null,"abstract":"Thiol/disulfide-based redox regulation is a key mechanism for modulating protein functions in response to changes in cellular redox status. Two thioredoxin (Trx)-like proteins [atypical Cys His-rich Trx (ACHT) and Trx-like2 (TrxL2)] have been identified as crucial for oxidizing and deactivating several chloroplast enzymes during light-to-dark transitions; however, their roles remain to be fully understood. In this study, we investigated the functions of Trx-like proteins in seed development. Using the CRISPR/Cas9 system, we generated an Arabidopsis quadruple mutant defective in ACHT1, ACHT2, TrxL2.1, and TrxL2.2 (acht/trxl2). This mutant showed increased seed lethality prior to maturation, with embryogenesis impaired primarily during the heart and torpedo stages, which are critical phases for plastid differentiation into chloroplasts. Using transgenic plants expressing EGFP-fused proteins, we confirmed that ACHT and TrxL2 are localized in plastids during embryogenesis. Additionally, seed development in the acht/trxl2 mutant was further impaired under extended darkness and could not be recovered through complementation with variants of ACHT or TrxL2 lacking the redox-active Cys residue (replaced by Ser). These findings indicate that the protein-oxidation functions of ACHT and TrxL2 are important for plastid differentiation into chloroplasts, embryogenesis, and seed development.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal changes in pigment content in overwintering and current-year leaves of Sasa senanensis from snowmelt to before leaf-fall of canopy deciduous trees.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-17 DOI: 10.1007/s10265-024-01607-3

Kiyomi Ono, Megumi Hashiguchi, Ryouichi Tanaka, Toshihiko Hara

{"title":"Seasonal changes in pigment content in overwintering and current-year leaves of Sasa senanensis from snowmelt to before leaf-fall of canopy deciduous trees.","authors":"Kiyomi Ono, Megumi Hashiguchi, Ryouichi Tanaka, Toshihiko Hara","doi":"10.1007/s10265-024-01607-3","DOIUrl":"https://doi.org/10.1007/s10265-024-01607-3","url":null,"abstract":"Sasa senanensis (a dwarf bamboo), an evergreen herbaceous plant native to the cool temperate regions of eastern Asia, endures seasonal temperature fluctuations and significant variations in light intensity typical for understory plants. Following snowmelt in early spring, the light intensity received by Sasa leaves surges, then diminishes as the canopy of upper deciduous trees develops. The current-year leaves of S. senanensis unfold under these shaded conditions, rendering the preservation of overwintering leaves vital for maintaining photosynthetic productivity in early spring. This study investigated the adaptations of overwintering leaves of S. senanensis to the low temperatures and elevated light conditions typical of early spring, examining whether these leaves dissipate absorbed light energy as heat and/or reduce their antenna size in response to increased light levels. Comprehensive analyses of Fv/Fm and photosynthetic pigment compositions were conducted throughout the spring to autumn seasons from 2014 to 2017. Our results indicate that Fv/Fm in overwintering leaves was initially low in early spring but increased gradually before the onset of shading, maintaining high levels under shaded conditions across all examined years. The chlorophyll a/b ratio increased post-snowmelt and decreased with intensified shading annually, with the exception of 2015, suggesting that reductions in antenna size are not essential for Fv/Fm recovery. Furthermore, the quantities and de-epoxidation state of xanthophyll cycle pigments increased after snowmelt despite rising temperatures, then decreased with progressive shading each year, indicating that overwintering leaves adapt to early spring conditions by modulating their xanthophyll cycle pigments. This study demonstrates that the overwintering leaves of S. senanensis exhibit a flexible response in photosystem pigments to variations in the light environment.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142835904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic and quantitative properties of Rubisco in some conifers and lycopods.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-14 DOI: 10.1007/s10265-024-01606-4

Sakiko Sugawara, Kana Ito, Shin-Ichi Miyazawa, Amane Makino, Yuji Suzuki

{"title":"Enzymatic and quantitative properties of Rubisco in some conifers and lycopods.","authors":"Sakiko Sugawara, Kana Ito, Shin-Ichi Miyazawa, Amane Makino, Yuji Suzuki","doi":"10.1007/s10265-024-01606-4","DOIUrl":"https://doi.org/10.1007/s10265-024-01606-4","url":null,"abstract":"Information on the kinetic properties of Rubisco, a key enzyme for photosynthesis, is scarce in land plants that emerged early during the evolutionary process. This study examined the carboxylase activity and abundance of Rubisco in five conifers, two lycopods, and three control C3 crops. The turnover rates of Rubisco carboxylation (kcatc) under saturated-CO2 conditions in conifers and lycopods were comparable to those in the control C3 crops. Rubisco carboxylase activity under CO2-unsaturated conditions (vcu) was also measured using reaction mixtures saturated with a N2 gas containing CO2 and O2 at present atmospheric levels to predict the Rubisco CO2 affinity from the percentage of vcu in kcatc. The predicted CO2 affinity in conifers and lycopods tended to be lower than that in the control C3 crops. When the control C3 crops and two previously examined C4 crops were analyzed together, the kcatc of Rubisco with a low CO2 affinity tended to be high. N allocation to Rubisco with a low kcatc tended to be high in these plants. In conifers and lycopods, the kcatc was lower than that expected on the basis of predicted Rubisco CO2 affinity, unlike in the control crops. N allocation to Rubisco also tended to be lower than that expected on the basis of kcatc. These results indicate that Rubisco in the examined conifers and lycopods is not superior in terms of both kcatc and CO2 affinity and that the abundance of Rubisco is not necessarily closely related to its kinetic properties. The reason for these phenomena is discussed in terms of the molecular evolution of Rubisco.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATML1 and PDF2 regulate cuticle formation and protect the plant body from environmental stresses in Arabidopsis thaliana seedlings.

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2024-12-10 DOI: 10.1007/s10265-024-01604-6

Kenji Nagata, Ichiro Maekawa, Taku Takahashi, Mitsutomo Abe

{"title":"ATML1 and PDF2 regulate cuticle formation and protect the plant body from environmental stresses in Arabidopsis thaliana seedlings.","authors":"Kenji Nagata, Ichiro Maekawa, Taku Takahashi, Mitsutomo Abe","doi":"10.1007/s10265-024-01604-6","DOIUrl":"https://doi.org/10.1007/s10265-024-01604-6","url":null,"abstract":"A sessile lifestyle compels plants to endure an array of environmental stressors in the location where they grow. To cope with environmental stresses, plants have developed specialized cell wall structures called cuticles at the interface between the plant and the environment. In Arabidopsis thaliana seedlings, cuticles cover and protect aerial organs and young roots. However, the precise assembly of the molecular machinery required for cuticle formation on the surface of distinct organs that exhibit entirely different functions and developmental contexts remains unknown. Here, we demonstrate that a paralogous gene pair, ARABIDOPSIS THALIANA MERISTEM LAYER1 (ATML1) and PROTODERMAL FACTOR2 (PDF2), regulates precise cuticle formation in Arabidopsis thaliana seedlings. We found that the expression of ATML1 and PDF2 spatially overlapped with cuticle deposition in Arabidopsis thaliana seedlings. Furthermore, the loss of ATML1 and PDF2 activity resulted in a significant downregulation of the expression of genes required for cuticle formation and compromised cuticle formation in different organs. Seedlings with impaired activities of ATML1 and PDF2 exhibited higher susceptibility to environmental stress. In particular, PDF2 plays a predominant role in tolerance to environmental stress rather than ATML1 in the roots. Collectively, our study provides new insights into the regulatory mechanisms of cuticle formation and the developmental strategies plants use to protect their bodies from environmental stresses.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0