Journal of Plant Research最新文献

{"title":"Equisetum praealtum and E. hyemale have abundant Rubisco with a high catalytic turnover rate and low CO2 affinity","authors":"Kana Ito, Sakiko Sugawara, Sota Kageyama, Naoki Sawaguchi, Takuro Hyotani, Shin-Ichi Miyazawa, Amane Makino, Yuji Suzuki","doi":"10.1007/s10265-023-01514-z","DOIUrl":"https://doi.org/10.1007/s10265-023-01514-z","url":null,"abstract":"<p>The kinetic properties of Rubisco, a key enzyme for photosynthesis, have been examined in numerous plant species. However, this information on some plant groups, such as ferns, is scarce. This study examined Rubisco carboxylase activity and leaf Rubisco levels in seven ferns, including four <i>Equisetum</i> plants (<i>E. arvense</i>, <i>E. hyemale</i>, <i>E. praealtum</i>, and <i>E. variegatum</i>), considered living fossils. The turnover rates of Rubisco carboxylation (<i>k</i>_cat^c) in <i>E. praealtum</i> and <i>E. hyemale</i> were comparable to those in the C₄ plants maize (<i>Zea mays</i>) and sorghum (<i>Sorghum bicolor</i>), whose <i>k</i>_cat^c values are high. Rubisco CO₂ affinity, estimated from the percentage of Rubisco carboxylase activity under CO₂ unsaturated conditions in <i>k</i>_cat^c in these <i>Equisetum</i> plants, was low and also comparable to that in maize and sorghum. In contrast, <i>k</i>_cat^c and CO₂ affinities of Rubisco in other ferns, including <i>E. arvense</i> and <i>E. variegatum</i> were comparable with those in C₃ plants. The N allocation to Rubisco in the ferns examined was comparable to that in the C₃ plants. These results indicate that <i>E. praealtum</i> and <i>E. hyemale</i> have abundant Rubisco with high <i>k</i>_cat^c and low CO₂ affinity, whereas the carboxylase activity and abundance of Rubisco in other ferns were similar to those in C₃ plants. Herein, the Rubisco properties of <i>E. praealtum</i> and <i>E. hyemale</i> were discussed regarding their evolution and physiological implications.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138740851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive interference between alien species in Veronica","authors":"Sachiko Nishida, Naoko Tamakoshi, Ko-Ichi Takakura, Yuta Watanabe, Masahiro M. Kanaoka","doi":"10.1007/s10265-023-01510-3","DOIUrl":"https://doi.org/10.1007/s10265-023-01510-3","url":null,"abstract":"<p>Reproductive interference can lead to the exclusive distribution of species. Reports on reproductive interference between alien species are scarce, although alien species are becoming more abundant and evaluations of the interference between aliens and its influence on community structure are essential. We therefore investigated the presence of interference in two alien species of <i>Veronica</i>, <i>V. cymbalaria</i> and <i>V. persica</i>. Hand-pollination experiments revealed that heterospecific pollen adversely affected both species, but asymmetrically: in <i>V. cymbalaria</i>, it reduced the number of seeds both before and after conspecific pollination, but in <i>V. persica</i> it reduced the number of seeds only when it occurred before conspecific pollination. Field observation suggested that the number of seeds produced by <i>V. cymbalaria</i> was more influenced by the number of conspecific flowers than by the frequency of surrounding <i>V. persica</i> flowers. Pot experiments did not show a negative effect of heterospecific individuals on the reproductive success of focal species in either species. Observations of visiting pollinators revealed no pollinator preference between the plant species. Although we demonstrated asymmetrical reproductive interference between <i>V. cymbalaria</i> and <i>V. persica</i>, its effect in the field seemed limited. An essential factor mitigating the adverse effect of reproductive interference might be a shortage of pollinators that could facilitate interspecific pollination. We predict that further invasion of <i>V. cymbalaria</i> into areas where <i>V. persica</i> prevails is unlikely, although swift displacement of <i>V. cymbalaria</i> by <i>V. persica</i> in areas where <i>V. cymbalaria</i> is already established is also unlikely.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138714531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative floral development in Mimosa (Fabaceae: Caesalpinioideae) brings new insights into merism lability in the mimosoid clade","authors":"Bruno Cesar Ferreira Gonçalves, Vidal de Freitas Mansano, Renan Siqueira de Moraes, Juliana Villela Paulino","doi":"10.1007/s10265-023-01507-y","DOIUrl":"https://doi.org/10.1007/s10265-023-01507-y","url":null,"abstract":"<p>The genus <i>Mimosa</i> L. (Leguminosae; Caesalpinioideae; mimosoid clade), comprising more than 500 species, is an intriguing genus because, like other members of the mimosoid clade, it presents an enormous variation in floral characteristics and high merism lability. Thus, this study aimed to elucidate the floral development and identify which ontogenetic pathways give rise to merism variation and andromonoecy in <i>Mimosa caesalpiniifolia, M. pudica, M. bimucronata</i>, and <i>M. candollei</i>. Floral buds at various stages of development and flowers were collected, fixed, and processed for surface analysis (SEM). The development of the buds is synchronous in the inflorescences. Sepals appear simultaneously as individualized primordia in <i>M. caesalpiniifolia</i> and in reversed unidirectional order in <i>M. bimucronata</i>, with union and formation of an early ring-like calyx. Petal primordia appear in unidirectional order, with a noticeably elliptical shape in <i>M. caesalpiniifolia</i>. The wide merism variation in <i>Mimosa</i> results from the absence of organs from inception in the perianth and androecium whorls: in dimerous, trimerous, or tetramerous flowers, the additional organs primordia to compose the expected pentamerous flowers are not initiated. The haplostemonous androecium of <i>M. pudica</i> results from the absence of antepetalous stamens from inception. In the case of intraspecific variations (instabilities), there is no initiation and subsequent abortion of organs in the events of reduction in merosity. In addition, extra primordia are initiated in supernumerary cases. On the other hand, staminate flowers originate from the abortion of the carpel. <i>Mimosa</i> proved to be an excellent model for studying merism variation. The lability is associated with actinomorphic and rather congested flowers in the inflorescences. Our data, in association with others of previous studies, suggest that the high lability in merism appeared in clades that diverged later in the mimosoid clade. Thus, phylogenetic reconstruction studies are needed for more robust evolutionary inferences. The present investigation of ontogenetic processes was relevant to expand our understanding of floral evolution in the genus <i>Mimosa</i> and shed light on the unstable merism in the mimosoid clade.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138561646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Heterologous gene expression system for the production of hydrolyzable tannin intermediates in herbaceous model plants.","authors":"Chihiro Oda-Yamamizo,&nbsp;Nobutaka Mitsuda,&nbsp;Carsten Milkowski,&nbsp;Hideyuki Ito,&nbsp;Kentaro Ezura,&nbsp;Ko Tahara","doi":"10.1007/s10265-023-01488-y","DOIUrl":"10.1007/s10265-023-01488-y","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10014833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolome expression in Eucryphia cordifolia populations: Role of seasonality and ecological niche centrality hypothesis.","authors":"Camila Andrea Fuica-Carrasco,&nbsp;Óscar Toro-Núñez,&nbsp;Andrés Lira-Noriega,&nbsp;Andy J Pérez,&nbsp;Víctor Hernández","doi":"10.1007/s10265-023-01483-3","DOIUrl":"10.1007/s10265-023-01483-3","url":null,"abstract":"<p><p>The ecological niche centrality hypothesis states that population abundance is determined by the position in the ecological niche, expecting higher abundances towards the center of the niche and lower at the periphery. However, the variations in the conditions that favor the persistence of populations between the center and the periphery of the niche can be a surrogate of stress factors that are reflected in the production of metabolites in plants. In this study we tested if metabolomic similarity and diversity in populations of the tree species Eucryphia cordifolia Cav. vary according to their position with respect to the structure of the ecological niche. We hypothesize that populations growing near the centroid should exhibit lower metabolites diversity than plants growing at the periphery of the niche. The ecological niche of the species was modeled using correlative approaches and bioclimatic variables to define central and peripheral localities from which we chose four populations to obtain their metabolomic information using UHPLC-DAD-QTOF-MS. We observed that populations farther away from the centroid tend to have higher metabolome diversity, thus supporting our expectation of the niche centrality hypothesis. Nonetheless, the Shannon index showed a marked variation in metabolome diversity at the seasonal level, with summer and autumn being the periods with higher metabolite diversity compared to winter and spring. We conclude that both the environmental variation throughout the year in combination with the structure of the ecological niche are relevant to understand the variation in expression of metabolites in plants.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10155928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous gene expression system for the production of hydrolyzable tannin intermediates in herbaceous model plants.","authors":"Chihiro Oda-Yamamizo,&nbsp;Nobutaka Mitsuda,&nbsp;Carsten Milkowski,&nbsp;Hideyuki Ito,&nbsp;Kentaro Ezura,&nbsp;Ko Tahara","doi":"10.1007/s10265-023-01484-2","DOIUrl":"10.1007/s10265-023-01484-2","url":null,"abstract":"<p><p>Aluminum toxicity is the main factor limiting the elongation of plant roots in acidic soil. The tree species Eucalyptus camaldulensis is considerably more resistant to aluminum than herbaceous model plants and crops. Hydrolyzable tannins (HTs) accumulating in E. camaldulensis roots can bind and detoxify the aluminum taken up by the roots. However, in herbaceous model plants, HTs do not accumulate and the genes involved in the HT biosynthetic pathway are largely unknown. The aim of this study was to establish a method for reconstituting the HT biosynthetic pathway in the HT non-accumulating model plant Nicotiana benthamiana. Four E. camaldulensis enzymes were transiently expressed in N. benthamiana leaves via Agrobacterium tumefaciens-mediated transformation. These enzymes included dehydroquinate dehydratase/shikimate dehydrogenases (EcDQD/SDH2 and EcDQD/SDH3), which catalyze the synthesis of gallic acid, the first intermediate of the HT biosynthetic pathway that branches off from the shikimate pathway. The others were UDP-glycosyltransferases (UGT84A25 and UGT84A26), which catalyze the conversion of gallic acid to β-glucogallin, the second intermediate. The co-expression of the EcDQD/SDHs in transgenic N. benthamiana leaf regions promoted the synthesis of gallic acid. Moreover, the co-expression of the UGT84As in addition to the EcDQD/SDHs resulted in the biosynthesis of β-glucogallin, the universal metabolic precursor of HTs. Thus, we successfully reconstituted a portion of the HT biosynthetic pathway in HT non-accumulating N. benthamiana plants. This heterologous gene expression system will be useful for co-expressing candidate genes involved in downstream reactions in the HT biosynthetic pathway and for clarifying their in planta functions.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10002446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of phosphorus stress on the growth and secondary metabolism of Artemisia argyi.","authors":"Zixin Wang,&nbsp;Lin Ma,&nbsp;Changjie Chen,&nbsp;Lujuan Guo,&nbsp;Lanping Guo,&nbsp;Tingting Zhao,&nbsp;Dahui Liu","doi":"10.1007/s10265-023-01479-z","DOIUrl":"10.1007/s10265-023-01479-z","url":null,"abstract":"<p><p>Phosphorus is essential in critical plant processes such as signaling, photosynthesis, energy metabolism, and enzyme activity during respiration. Phosphorus stress therefore has a significant impact on plant growth and metabolism. Here, we characterized the biochemical responses of Artemisia argyi Level. et Vant to low phosphorus (LP) and high phosphorus (HP) stress. Plants were treated with 0 g (LP), 1.5 g (control), or 3 g (HP) P per 10 kg of soil. The results demonstrated that CK encouraged the most plant growth, as quantified by leaf size and plant biomass. We also found that the total amounts of phenolic and flavonoid compounds (such as chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, cryptochlorogenic acid, neochlorogenic acid, hispidulin, jaceosidin, eupatilin, and casticin) were increased in the leaves of A. argyi plants exposed to LP stress compared to those raised under CK conditions. The levels of these compounds were inversely related to the amount of phosphorus added, and therefore peaked in plants treated with LP stress. Levels of terpenoids were also found to fluctuate under LP and HP stress compared to CK conditions. Furthermore, transcriptomic analyses showed up-regulation of several genes encoding key enzymes in the flavonoid and phenolic acid metabolic pathways under LP stress. There were also alterations in the expression levels of genes in the methylerythritol 4-phosphate and mevalonate pathways of terpene synthesis. This study contributes to a deeper understanding of the physiological and molecular mechanisms underlying phosphorus stress responses and their impacts on the growth and quality of the economically important species A. argyi.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9927522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycorrhizal specificity differences in epiphytic habitat: three epiphytic orchids harbor distinct ecological and physiological specificity.","authors":"Kento Rammitsu,&nbsp;Masaru Goto,&nbsp;Yumi Yamashita,&nbsp;Tomohisa Yukawa,&nbsp;Yuki Ogura-Tsujita","doi":"10.1007/s10265-023-01486-0","DOIUrl":"10.1007/s10265-023-01486-0","url":null,"abstract":"<p><p>Orchidaceae has diversified in tree canopies and accounts for 68% of vascular epiphytes. Differences in mycorrhizal communities among epiphytic orchids can reduce species competition for mycorrhizal fungi and contribute to niche partitioning, which may be a crucial driver of the unusual species diversification among orchids. Mycorrhizal specificity-the range of fungi allowing mycorrhizal partnerships-was evaluated by assessment of mycorrhizal communities in the field (ecological specificity) and symbiotic cultures in the laboratory (physiological specificity) for three epiphytic orchids inhabiting Japan. Mycorrhizal communities were assessed with co-existing individuals growing within 10 cm of each other, revealing that ecological specificity varied widely among the three species, ranging from dominance by a single Ceratobasidiaceae fungus to diverse mycobionts across the Ceratobasidiaceae and Tulasnellaceae. In vitro seed germination tests revealed clear differences in physiological specificity among the three orchids, and that the primary mycorrhizal partners contributed to seed germination. In vitro compatibility ranges of three orchids strongly reflect the mycorrhizal community composition of wild populations. This suggests that differences in in situ mycorrhizal communities are not strongly driven by environmental factors, but are primarily due to physiological differences among orchid species. This study shows that the symbiotic strategy among the epiphytic orchid species varies from specialized to generalized association, which may contribute to biotic niche partitioning.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10035828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of mycoheterotrophy on the growth of Gentiana zollingeri (Gentianaceae), as suggested by size variation, morphology, and ¹³C abundance of flowering shoots.","authors":"Masahide Yamato,&nbsp;Mai Yagita,&nbsp;Ryota Kusakabe,&nbsp;Keito Shimabukuro,&nbsp;Kohei Yamana,&nbsp;Kenji Suetsugu","doi":"10.1007/s10265-023-01496-y","DOIUrl":"10.1007/s10265-023-01496-y","url":null,"abstract":"<p><p>Gentiana zollingeri is an annual photosynthetic plant that employs a mycoheterotrophic growth strategy during its underground seedling stage (initial mycoheterotrophy). Notably, the morphological characteristics of its flowering shoots, such as shoot size, leaf size, and leaf color, are highly variable, and it was hypothesized that these variations may be linked to nutritional mode. The morphological characteristics of G. zollingeri individuals were thus investigated alongside environmental factors, ¹³C abundance, and diversity of colonizing arbuscular mycorrhizal (AM) fungi. The majority of G. zollingeri flowering individuals were found to exhibit a high affinity for the specific AM fungi that exclusively colonize roots of the mycoheterotrophic seedlings, while other phylogenetically diverse AM fungi could also be detected. The leaves to shoot dry weight ratio (leaf ratio) was negatively correlated with the canopy openness in the habitat, suggesting that leaf development is impeded in sunny conditions. Furthermore, the shoot weight of G. zollingeri was positively correlated with leaf ¹³C abundance. Given that ¹³C enrichment can provide indirect evidence of mycoheterotrophy in AM plants, the results suggest that the utilization of carbon obtained through mycoheterotrophy, at least during the underground seedling stage, is crucial for G. zollingeri.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10242800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Metabolome expression in Eucryphia cordifolia populations: role of seasonality and ecological niche centrality hypothesis.","authors":"Camila Fuica-Carrasco,&nbsp;Óscar Toro-Núñez,&nbsp;Andrés Lira-Noriega,&nbsp;Andy J Pérez,&nbsp;Víctor Hernández","doi":"10.1007/s10265-023-01489-x","DOIUrl":"10.1007/s10265-023-01489-x","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10113808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}