A unique phytochrome B gene in Cuscuta campestris and its responses during the initial stage of haustorium formation.

IF 2.7 3区 生物学 Q2 PLANT SCIENCES
Toshiya Yokoyama, Mariko Asaoka, Kazuhiko Nishitani
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引用次数: 0

Abstract

Cuscuta, a genus of stem-parasitic plants, requires specific light and touch stimuli for host recognition and haustorium formation. Blue and far-red light promote parasitism, while red light inhibits it, but the roles played by light-responsive genes in the early stages of Cuscuta parasitism remain largely unexplored. This study utilized an in vitro C. campestris haustorium induction system to investigate this issue. RNA-sequence analysis revealed a group of differentially expressed genes that responded to the initiation of haustorium formation. Genes associated with light-signaling pathways, including phytochromes (PHYs), phytochrome-interacting factors (PIFs), and homeodomain-leucine zipper I/II transcription factors, were specifically up or downregulated in plants exposed to light for 4 h. Phylogenetic analysis demonstrated that two distinct forms of the PHYB gene, CcPHYB1 and CcPHYB2, were present in all sequenced Cuscuta species. CcPHYB2 was not expressed under normal light conditions, but was specifically upregulated under light regimes inducing haustorium formation; by contrast, CcPHYB1 exhibited non-light-dependent expression. Furthermore, the induction of haustorium formation by far-red light was canceled by red light exposure but could be re-activated by subsequent re-exposure to far-red light. The levels of expression of CcPHYB2, CcPIF1, CcPIF4, CcHOMEOBOX FROM ARABIDOPSIS THALIANA 3 (CcHAT3), CcHOMEOBOX 2 (CcHB2) and CcHB13 varied under different haustorium-inducing light conditions. Expression of these genes altered significantly upon exposure to blue and far-red light, but these changes were reversed in plants exposed to red light. These findings highlight the pivotal role of light-responsive signaling during the early stages of parasitism, providing valuable insights into the molecular mechanisms and evolution of light-mediated host recognition and parasitism in Cuscuta species.

油菜中独特的光敏色素B基因及其在吸器形成初期的反应。
菟丝子是一种茎寄生植物,需要特定的光和触觉刺激来识别寄主和吸器的形成。蓝光和远红光促进寄主,而红光抑制寄主,但光反应基因在Cuscuta寄主早期阶段所起的作用仍未得到充分研究。本研究利用体外campestris吸器诱导系统对这一问题进行了研究。rna序列分析揭示了一组响应吸器形成起始的差异表达基因。与光信号通路相关的基因,包括光敏色素(phyys)、光敏色素相互作用因子(pif)和同源结构域亮氨酸zipper I/II转录因子,在暴露于光4小时的植物中特异性上调或下调。系统发育分析表明,所有测序的Cuscuta物种中都存在两种不同形式的PHYB基因,CcPHYB1和CcPHYB2。CcPHYB2在正常光照条件下不表达,但在诱导吸器形成的光照条件下特异性上调;相比之下,CcPHYB1表现出非光依赖性表达。此外,远红光对吸器形成的诱导作用被红光照射所抵消,但可以在随后的远红光照射下重新激活。拟南芥3 (CcHAT3)、CcHOMEOBOX 2 (CcHB2)和CcHB13中CcPHYB2、CcPIF1、CcPIF4、CcHOMEOBOX在不同吸器诱导光条件下的表达水平不同。这些基因的表达在暴露于蓝光和远红光时发生了显著变化,但这些变化在暴露于红光的植物中被逆转。这些发现强调了光响应信号在寄主早期阶段的关键作用,为光介导寄主识别和寄主在菟丝子物种中的分子机制和进化提供了宝贵的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Plant Research
Journal of Plant Research 生物-植物科学
CiteScore
5.40
自引率
3.60%
发文量
59
审稿时长
1 months
期刊介绍: The Journal of Plant Research is an international publication that gathers and disseminates fundamental knowledge in all areas of plant sciences. Coverage extends to every corner of the field, including such topics as evolutionary biology, phylogeography, phylogeny, taxonomy, genetics, ecology, morphology, physiology, developmental biology, cell biology, molecular biology, biochemistry, biophysics, bioinformatics, and systems biology. The journal presents full-length research articles that describe original and fundamental findings of significance that contribute to understanding of plants, as well as shorter communications reporting significant new findings, technical notes on new methodology, and invited review articles.
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