百合生殖细胞染色质结构和转录活性的动态变化。

IF 2.7 3区 生物学 Q2 PLANT SCIENCES
Mio K Shibuta, Tsugumi Aso, Yutsuki Okawa
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引用次数: 0

摘要

花粉是受精和相关的种子和果实生产所必需的,这对人类的营养很重要。对拟南芥(Arabidopsis thaliana)三细胞花粉的研究发现,精子细胞中的染色质高度凝聚,转录活性受到抑制。然而,尚未对双细胞花粉的生殖细胞进行全面的结构研究。在这项研究中,我们为其他产生双细胞花粉的被子植物提供了相关的见解。以长花百合为研究对象,对其花粉和花粉管的染色质结构和转录活性区进行了详细的分析。染色质被浓缩,形成在成熟的生殖细胞核中清晰可见的带状结构。此外,当花粉干燥时,转录活性区域仅限于染色质交叉处。虽然发现在花粉管生长过程中不需要从头转录,但在花粉管生长过程中,转录活性在生殖细胞分裂前暂时恢复。此外,从头转录的抑制影响了核形态的变化。本研究阐明了双细胞花粉生殖细胞核中不同的染色质结构和转录活性状态,这些数据有助于深入了解花粉成熟和花粉管生长的转录和其他调控机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dynamic changes in chromatin structure and transcriptional activity in the generative cells of Lilium longiflorum.

Pollen is required for fertilization and the associated production of seeds and fruits, which are important for human nutrition. Research on the tricellular pollen of Arabidopsis thaliana revealed that chromatin is highly condensed and transcriptional activity is suppressed in sperm cells. However, comprehensive structural investigations involving generative cells of bicellular pollen have not been conducted. In this study, we provide relevant insights into other angiosperms that produce bicellular pollen. Lilium longiflorum, which has large and easily observable nuclei, was used for a detailed analysis of the chromatin structure and transcriptionally active regions in pollen and pollen tubes. Chromatin was condensed, resulting in a ribbon-like structure that was clearly visible in mature generative cell nuclei. Additionally, transcriptionally active regions were restricted to the intersections of chromatin as pollen desiccated. Although de novo transcription was revealed to be unnecessary for pollen tube growth, transcriptional activity temporarily resumed before generative cell division during pollen tube growth. Moreover, the inhibition of de novo transcription influenced changes in nuclear morphology. In this study, the distinctive chromatin structures and transcriptional activity states in generative cell nuclei of bicellular pollen were elucidated, with the generated data contributing to a deeper understanding of transcription and other regulatory mechanisms involved in pollen maturation and pollen tube growth.

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来源期刊
Journal of Plant Research
Journal of Plant Research 生物-植物科学
CiteScore
5.40
自引率
3.60%
发文量
59
审稿时长
1 months
期刊介绍: The Journal of Plant Research is an international publication that gathers and disseminates fundamental knowledge in all areas of plant sciences. Coverage extends to every corner of the field, including such topics as evolutionary biology, phylogeography, phylogeny, taxonomy, genetics, ecology, morphology, physiology, developmental biology, cell biology, molecular biology, biochemistry, biophysics, bioinformatics, and systems biology. The journal presents full-length research articles that describe original and fundamental findings of significance that contribute to understanding of plants, as well as shorter communications reporting significant new findings, technical notes on new methodology, and invited review articles.
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