Journal of Plant Research最新文献_第5页

The assembly of a Malus sieversii regulatory network reveals gene resistance against Alternaria alternata f. sp. mali when colonized by Trichoderma biofertilizer. 海参调控网络的组装揭示了当木霉生物肥料定殖时对马利交替稻瘟菌的基因抗性。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-05-01 Epub Date: 2025-03-15 DOI: 10.1007/s10265-025-01622-y

Shida Ji, Dechen Li, Jin Yao, Bin Liu, Jing Han, Yucheng Wang, Zhihua Liu

{"title":"The assembly of a Malus sieversii regulatory network reveals gene resistance against Alternaria alternata f. sp. mali when colonized by Trichoderma biofertilizer.","authors":"Shida Ji, Dechen Li, Jin Yao, Bin Liu, Jing Han, Yucheng Wang, Zhihua Liu","doi":"10.1007/s10265-025-01622-y","DOIUrl":"10.1007/s10265-025-01622-y","url":null,"abstract":"Trichoderma spp., as excellent biocontrol agents, can induce systemic resistance to protect plants from phytopathogen attacks. In a previous study, Trichoderma biofertilizer activated the MsERF105 transcription factor (TF), which further enhanced the resistance of Malus sieversii against Alternaria alternata f. sp. mali, but how resistance signals are transmitted is still unknown. In this study, it was found that the MsERF105-centered disease-resistant regulatory network was induced by Trichoderma in M. sieversii. The TF-centered yeast one-hybrid indicated that WRKY33 and WRKY40 bound to WBOXATNPR1 elements and GT1 bound to GT1CONSENSUS elements in the promoter of MsERF105 to activate its expression. In addition, the proteins that interacted with MsERF105 were identified by yeast two-hybrid, including FUBP2 and HSP17.8. Furthermore, the candidate target genes of MsERF105 were screened using RNA-Seq, and yeast one-hybrid and tobacco transient transformation further showed MsERF105 bound to GCCBOX elements to regulate the expression of bHLH162, ERF017, NAC83 and NAC104; bound to CCAATBOX elements to regulate the expression of HSFs, HSP70s and HSP20; and bound to ERS elements to regulate the expression of DRPs. Finally, the Trichoderma-induced MsERF105-centered regulatory network of M. sieversii against A. alternata f. sp. mali was built, which provided reliable theoretical guidance for the application of Trichoderma and the disease-resistance breeding of M. sieversii.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"483-496"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Devastating impact of the seed-feeding fly Japanagromyza tokunagai on the endangered orchid Cephalanthera falcata. 食籽蝇对濒危兰花falcata的破坏性影响。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-05-01 Epub Date: 2025-03-03 DOI: 10.1007/s10265-025-01623-x

Kenji Suetsugu, Rikiya Kurashige, Shigeki Fukushima

{"title":"Devastating impact of the seed-feeding fly Japanagromyza tokunagai on the endangered orchid Cephalanthera falcata.","authors":"Kenji Suetsugu, Rikiya Kurashige, Shigeki Fukushima","doi":"10.1007/s10265-025-01623-x","DOIUrl":"10.1007/s10265-025-01623-x","url":null,"abstract":"Numerous studies have examined the reproductive systems of threatened orchids to develop effective conservation strategies. However, the detrimental effects of seed predators on seed production are often overlooked. In this study, we evaluated the impact of the seed-parasitic fly Japanagromyza tokunagai on the seed production of the endangered orchid Cephalanthera falcata, based on observations from five locations over one year and from a single location over four years in Chiba Prefecture, Japan. Our findings showed that J. tokunagai caused 100% capsule damage across all sites and years examined. Although some infested fruits still produced seeds in certain locations and years, the quantities were very low. Consequently, we observed a 99.1-100% reduction in seed production across all populations investigated. These results suggest that reduced seed production could limit generational turnover, potentially threatening the reproductive success and long-term survival of C. falcata, at least in the populations studied. This highlights the need to mitigate the negative impact of J. tokunagai on seed production in C. falcata. Combining artificial pollination with the bagging of individual inflorescences could be an effective approach, capable of increasing seed production by more than 100-fold. Given the fungal dependence and low germination rates of C. falcata, future work should also examine seedling recruitment to better understand the impacts of seed loss and improve estimates of long-term resilience.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"459-467"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights into pollen release and presentation in legumes: the case of Myroxylon peruiferum, a papilionoid with non-papilionaceous flowers. 豆科植物花粉释放和表现的新认识：以一种具有非凤蝶花的类凤蝶为例。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-05-01 Epub Date: 2025-03-28 DOI: 10.1007/s10265-025-01629-5

Viviane Gonçalves Leite, Vidal Freitas Mansano, Simone Pádua Teixeira

{"title":"New insights into pollen release and presentation in legumes: the case of Myroxylon peruiferum, a papilionoid with non-papilionaceous flowers.","authors":"Viviane Gonçalves Leite, Vidal Freitas Mansano, Simone Pádua Teixeira","doi":"10.1007/s10265-025-01629-5","DOIUrl":"10.1007/s10265-025-01629-5","url":null,"abstract":"The species-rich legume family displays diverse mechanisms for pollen presentation and release, including brush, piston, valvular, and explosive types, influenced by variations in floral architecture. Among papilionoids, a group characterized by flag-flowers, early-branching species often deviate from this typical pattern. This study investigates Myroxylon peruiferum L.f., an early-branching papilionoid legume native to the Brazilian Atlantic Forest, with non-papilionaceous flowers. Through detailed macromorphological, anatomical, histochemical, and ultrastructural analyses of floral organs, we reveal new insights into pollen release and presentation mechanisms in legumes. Pollen is released through an unusual process: the anther opens via an apical wide slit that gradually extends toward the base, releasing pollen in stages. Ducts in the sepals, petals, ovary, and anther secrete translucent oleoresin droplets that harden when released into the external environment. These serve multiple functions, including enhancing flower visibility, facilitating secondary pollen presentation by attaching pollen to the anther apex and petal tips, and acting as olfactory attractants due to their terpene content. M. peruiferum presents several unique traits not previously described in this subclade, including (a) oleoresin overflow through anther pores, (b) uncommon rimose anther dehiscence, and (c) a novel form of secondary pollen presentation via oleoresin drops. These findings provide important new insights into the reproductive strategy of this species and offer broader implications for legume biology.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"469-482"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The variation of summer heat resistance was associated with leaf transpiration rate in relatively large-leaf Rhododendron plants in southwest China. 西南地区大叶杜鹃花夏季耐热性的变化与叶片蒸腾速率有关。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-05-01 Epub Date: 2025-02-08 DOI: 10.1007/s10265-025-01620-0

Hai-Xia Zhang, Huie Li

{"title":"The variation of summer heat resistance was associated with leaf transpiration rate in relatively large-leaf Rhododendron plants in southwest China.","authors":"Hai-Xia Zhang, Huie Li","doi":"10.1007/s10265-025-01620-0","DOIUrl":"10.1007/s10265-025-01620-0","url":null,"abstract":"The summer heat is a vital factor limiting the introduction of relatively large-leaf Rhododendron plants to low-altitude areas, making it crucial to evaluate the resistance of different germplasm to summer heat. A pot experiment was conducted in 2023 to investigate the temporal changes in the photosynthetic characteristics, physiological and biochemical characteristics, and chlorophyll fluorescence characteristics of 14 representative relatively large-leaf Rhododendron germplasm. The results showed the R. irroratum and 'Hotspur Red' exhibited the highest heat damage index (HDI), while R. jiulongshanense and 'Moser Maroon' had the lowest HDI among the 14 Rhododendron germplasm. The photosynthesis rate and Fv/Fm (maximum photochemical efficiency) initially decreased and then recovered in all germplasm except R. irroratum. In contrast, the leaf transpiration rate, stomatal conductance, and chlorophyll content gradually increased. Hydrogen peroxide concentration first decreased and then increased, while malondialdehyde concentration initially increased and then decreased. Additionally, the superoxide anion content gradually increased. The activities of superoxide dismutase, peroxidase, and catalase (CAT) initially increased and then decreased. The HDI was positively correlated with CAT activity (r = 0.28, P < 0.05) but negatively correlated with photosynthesis rate (r = -0.26, P < 0.05), leaf transpiration rate (r = -0.27, P < 0.05), and Fv/Fm (r = -0.43, P < 0.001). Variation in summer heat resistance, as indicated by HDI, was observed among the 14 Rhododendron germplasm. This heat resistance was mainly associated with leaf transpiration rate and Fv/Fm. The indirect role of antioxidant enzymes in maintaining reactive oxygen species homeostasis in summer heat resistance was observed. The results provide a reference for introducing and cultivating relatively large-leaf Rhododendron plants to low-altitude areas.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"433-446"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wasp pollination and pollinator filtering by dense hairs at the floral tube entrance in Marsdenia tinctoria (Apocynaceae). 夹竹桃科花管入口处密毛对黄蜂授粉和传粉者的过滤作用。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-05-01 Epub Date: 2025-02-20 DOI: 10.1007/s10265-025-01621-z

Ko Mochizuki, Ayako Watanabe-Taneda

{"title":"Wasp pollination and pollinator filtering by dense hairs at the floral tube entrance in Marsdenia tinctoria (Apocynaceae).","authors":"Ko Mochizuki, Ayako Watanabe-Taneda","doi":"10.1007/s10265-025-01621-z","DOIUrl":"10.1007/s10265-025-01621-z","url":null,"abstract":"The physical filtering of pollinators is an important factor influencing pollination effectiveness. This study explored the potential functions of dense hairs that completely obstruct the entrance of floral tube in Marsdenia tinctoria by characterizing the flowers of this species, as well as its pollinators and their behavior. The corolla was white upon blooming in the morning, then turned yellow at night, and the flower finally dropped by the third morning. The hairs tended to be disheveled in yellow-petaled flowers. Pollination success increased with floral age. Direct observations of flowers in natural M. tinctoria populations over a period of 32 h recorded 126 visitors, of which 70% were wasps. We observed pollinia attached to the mouthparts of wasps, carpenter bees, and honeybees, but not to those of butterflies, moths, flies, or ants. Detailed examination of insect mouthparts and floral morphology indicated that insect visitors that acted as pollinators had mouthparts longer than the floral tubes, equipped with hairs to which pollinia could attach. The mouthparts of potter wasps were often covered with pollinaria, carrying on average 30-75 pollinia. The dense floral hairs were penetrated by large-bodied visitors, and blocked smaller visitors. Disturbance of these floral hairs allowed smaller insects to access nectar, suggesting that the hairs function in preventing nectar theft by smaller insects. This study presents the first case of wasp pollination in the genus Marsdenia and provides insights into the potential function of its dense floral hairs, a synapomorphy of this genus, in filtering floral visitors.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"447-457"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Needle/electrode insertion device for measuring plant electrical signals. 用于测量植物电信号的针/电极插入装置。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-05-01 Epub Date: 2025-03-04 DOI: 10.1007/s10265-025-01625-9

Miguel Ángel García Servín, Amanda Kim Rico Chávez, Magdalena Mendoza Sánchez, José Alfredo Acuña García, Ramón Gerardo Guevara González, Luis Miguel Contreras Medina

{"title":"Needle/electrode insertion device for measuring plant electrical signals.","authors":"Miguel Ángel García Servín, Amanda Kim Rico Chávez, Magdalena Mendoza Sánchez, José Alfredo Acuña García, Ramón Gerardo Guevara González, Luis Miguel Contreras Medina","doi":"10.1007/s10265-025-01625-9","DOIUrl":"10.1007/s10265-025-01625-9","url":null,"abstract":"Plants generate electrical signals in response to mild and severe environmental stimuli to transmit physiological information and ultimately trigger defensive responses during stressful events. It has been proposed that detecting and characterizing such signals could allow researchers to mimic specific electrical stimuli and provoke desirable responses in crops. Nevertheless, manually inserting electrodes in plant tissues leads to irregular data records due to a lack of uniformity across insertion events. For this reason, we manufactured a prototype of an electrode/needle insertion device built in aluminum and acrylic and used it to measure electrical signals in C. annuum plants. As a result, the device had more consistent insertion characteristics such as depth and alignment between electrodes and with plant stems. The device was also used to obtain electrical signals and compare them with the signals obtained using the traditional insertion technique, demonstrating that the use of the device promotes stability and repeatability in the captured signals.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"525-531"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Greetings from the new Editor-in-Chief of the Journal of Plant Research 2025. 《植物研究杂志2025》新任总编辑向您问好。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-04-07 DOI: 10.1007/s10265-025-01635-7

Noriko Inada

引用次数: 0

Synergistic effect of alkane and membrane lipid alteration in Synechococcus elongatus PCC 7942 under salt and light stresses. 盐和光胁迫下长聚球菌PCC 7942中烷烃和膜脂改变的协同效应

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-03-01 Epub Date: 2024-12-31 DOI: 10.1007/s10265-024-01613-5

Arif Agung Wibowo, Koichiro Awai

{"title":"Synergistic effect of alkane and membrane lipid alteration in Synechococcus elongatus PCC 7942 under salt and light stresses.","authors":"Arif Agung Wibowo, Koichiro Awai","doi":"10.1007/s10265-024-01613-5","DOIUrl":"10.1007/s10265-024-01613-5","url":null,"abstract":"Salinity and light markedly influence cyanobacterial viability. High salinity disrupts the osmotic balance, while excess light energy affects redox potential in the cells. Regulating the ratio of saturated and unsaturated alka(e)ne and fatty acids in cyanobacteria is thought to have crucial roles in coping with these stresses by regulating membrane fluidity. In Synechococcus elongatus PCC 7942 (Syn7942), alkane is produced from fatty acid metabolites using acyl-acyl carrier protein reductase (Aar) and aldehyde-deformylating oxygenase (Ado) enzymes. However, the role of alka(e)nes and their correlation with fatty acid-related compounds, especially under salinity stress, is not yet fully understood. This study explored the significance of the natural alka(e)ne biosynthesis pathway using Syn7942. The role of alka(e)ne was assessed using single and double knockout mutants of the aar and/or ado genes in this biosynthetic process. The alka(e)ne levels and membrane lipid content exhibited an inverse relationship, correlating with cell fluidity under high-salinity and high-light conditions. The absence of alka(e)ne resulted in a severe growth phenotype of Δado and Δaar/Δado under high-salinity conditions and less severe under high-light conditions. In addition, feeding with C15:0 and/or C17:0 alkanes complemented the growth phenotype with different accumulation profiles. The Δaar mutant exhibited higher resistance to high salinity than the Syn7942 WT, indicating the importance of Ado for survival at high salinity. Overall, lipid-related compounds, especially alka(e)nes, markedly contribute to cell integrity maintenance under high-salinity conditions by regulating membrane rigidity and fluidity.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"365-376"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal changes in pigment content in overwintering and current-year leaves of Sasa senanensis from snowmelt to before leaf-fall of canopy deciduous trees. 雪融雪期至冠层落叶树落叶前雪沙越冬叶和年叶色素含量的季节变化

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-03-01 Epub Date: 2024-12-17 DOI: 10.1007/s10265-024-01607-3

Kiyomi Ono, Megumi Hashiguchi, Ryouichi Tanaka, Toshihiko Hara

{"title":"Seasonal changes in pigment content in overwintering and current-year leaves of Sasa senanensis from snowmelt to before leaf-fall of canopy deciduous trees.","authors":"Kiyomi Ono, Megumi Hashiguchi, Ryouichi Tanaka, Toshihiko Hara","doi":"10.1007/s10265-024-01607-3","DOIUrl":"10.1007/s10265-024-01607-3","url":null,"abstract":"Sasa senanensis (a dwarf bamboo), an evergreen herbaceous plant native to the cool temperate regions of eastern Asia, endures seasonal temperature fluctuations and significant variations in light intensity typical for understory plants. Following snowmelt in early spring, the light intensity received by Sasa leaves surges, then diminishes as the canopy of upper deciduous trees develops. The current-year leaves of S. senanensis unfold under these shaded conditions, rendering the preservation of overwintering leaves vital for maintaining photosynthetic productivity in early spring. This study investigated the adaptations of overwintering leaves of S. senanensis to the low temperatures and elevated light conditions typical of early spring, examining whether these leaves dissipate absorbed light energy as heat and/or reduce their antenna size in response to increased light levels. Comprehensive analyses of Fv/Fm and photosynthetic pigment compositions were conducted throughout the spring to autumn seasons from 2014 to 2017. Our results indicate that Fv/Fm in overwintering leaves was initially low in early spring but increased gradually before the onset of shading, maintaining high levels under shaded conditions across all examined years. The chlorophyll a/b ratio increased post-snowmelt and decreased with intensified shading annually, with the exception of 2015, suggesting that reductions in antenna size are not essential for Fv/Fm recovery. Furthermore, the quantities and de-epoxidation state of xanthophyll cycle pigments increased after snowmelt despite rising temperatures, then decreased with progressive shading each year, indicating that overwintering leaves adapt to early spring conditions by modulating their xanthophyll cycle pigments. This study demonstrates that the overwintering leaves of S. senanensis exhibit a flexible response in photosystem pigments to variations in the light environment.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"215-230"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142835904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chlorophyll fluorescence responses to CO₂ availability reveal crassulacean acid metabolism in epiphytic orchids. 叶绿素荧光对CO2有效性的响应揭示了附生兰花的天冬酰胺酸代谢。

IF 2.7 3区生物学

Journal of Plant Research Pub Date : 2025-03-01 Epub Date: 2024-12-24 DOI: 10.1007/s10265-024-01608-2

Sae Bekki, Kenji Suetsugu, Koichi Kobayashi

{"title":"Chlorophyll fluorescence responses to CO2 availability reveal crassulacean acid metabolism in epiphytic orchids.","authors":"Sae Bekki, Kenji Suetsugu, Koichi Kobayashi","doi":"10.1007/s10265-024-01608-2","DOIUrl":"10.1007/s10265-024-01608-2","url":null,"abstract":"Crassulacean acid metabolism (CAM), a specialized mode of photosynthetic carbon assimilation characterized by nocturnal fixation of atmospheric CO2 and vacuolar malic acid storage, is found in a wide variety of vascular plant species, mainly those inhabiting water-limited environments. Identifying and characterizing diverse CAM species enhances our understanding of the physiological, ecological, and evolutionary significance of CAM photosynthesis. In this study, we examined the effect of CO2 elimination on chlorophyll fluorescence-based photosynthetic parameters in two constitutive CAM Kalanchoe species and six orchids. In CAM-performing Kalanchoe species, the effective quantum yield of photosystem II showed no change in response to CO2 elimination during the daytime but decreased with CO2 elimination at dusk. We applied this method to reveal the photosynthetic mode of epiphytic orchids and found that Gastrochilus japonicus, Oberonia japonica, and Bulbophyllum inconspicuum, but not B. drymoglossum, are constitutive CAM plants, which were also confirmed by malate determination. Our data propose a novel approach to identify and characterize CAM plants without labor-intensive experimental procedures. Although B. drymoglossum leaves had relatively high malate content, they did not depend on it to perform photosynthesis even under water-deficient or increased light conditions. Anatomical comparisons revealed a notable difference in leaf structure between B. drymoglossum and B. inconspicuum; B. drymoglossum leaves possess large water storage tissue internally, unlike B. inconspicuum leaves, which develop pseudobulbs. Our findings suggest different evolutionary adaptations to water deficit between closely related B. drymoglossum and B. inconspicuum.","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"323-336"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0