Journal of plant physiology最新文献_第3页

Barley root tip peroxidases convert DAF-FM and DAR-4M to an NO-independent fluorescent product using H2O2 derived from polyamine catabolism by polyamine oxidases 大麦根尖过氧化物酶利用多胺氧化酶分解代谢产生的H2O2将DAF-FM和DAR-4M转化为no独立的荧光产物

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-03-20 DOI: 10.1016/j.jplph.2025.154477

Katarína Valentovičová, Loriana Demecsová, Ľubica Liptáková, Veronika Zelinová, Ladislav Tamás

{"title":"Barley root tip peroxidases convert DAF-FM and DAR-4M to an NO-independent fluorescent product using H2O2 derived from polyamine catabolism by polyamine oxidases","authors":"Katarína Valentovičová, Loriana Demecsová, Ľubica Liptáková, Veronika Zelinová, Ladislav Tamás","doi":"10.1016/j.jplph.2025.154477","DOIUrl":"10.1016/j.jplph.2025.154477","url":null,"abstract":"<div><div>The aim of our study was to investigate the possible involvement of barley root tip peroxidases and polyamine oxidases in the conversion of DAF-FM or DAR-4M into an NO-independent fluorescent product after the exogenous application of polyamines. Application of spermidine or spermine into the incubation medium increased H<sub>2</sub>O<sub>2</sub> production by root tip segments in a dose-dependent manner. This spermidine- or spermine-induced increase in H<sub>2</sub>O<sub>2</sub> production was accompanied by intensified fluorescence of both DAF-FM and DAR-4M in a polyamine dose-dependent manner, similarly to exogenously added H<sub>2</sub>O<sub>2</sub>. On the contrary, exogenous putrescine neither evoked H<sub>2</sub>O<sub>2</sub> production nor increased DAF-FM or DAR-4M fluorescence. Application of guazatine, a polyamine oxidase inhibitor, into the incubation medium inhibited both H<sub>2</sub>O<sub>2</sub> production and DAF-FM or DAR-4M fluorescence. Spermidine- or spermine-induced DAF-FM or DAR-4M fluorescence decreased with an increasing amount of catalase or guaiacol, a competitive substrate for peroxidase, in the incubation medium. Exogenous application of indole-3-acetic acid, a well-known activator of NO generation in roots, but not of H<sub>2</sub>O<sub>2</sub>, spermidine or spermine, induces NO accumulation in the root tips. Exogenous application of spermidine or spermine to plant tissues with high polyamine oxidase and peroxidase activity, as are the barley root tips, generates an NO-independent fluorescence signal from either DAF-FM or DAR-4M, giving a false positive signal for NO emission.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154477"},"PeriodicalIF":4.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of the tomato nuclear-cytoplasmic shuttling bZIP transcription factor VSF-1 in Arabidopsis retards plant development under mannitol-stressed conditions 甘露醇胁迫下，拟南芥核胞质穿梭bZIP转录因子VSF-1的过度表达会延缓植株发育

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-03-17 DOI: 10.1016/j.jplph.2025.154476

Hyuk Sung Yoon , Daisuke Tsugama

{"title":"Overexpression of the tomato nuclear-cytoplasmic shuttling bZIP transcription factor VSF-1 in Arabidopsis retards plant development under mannitol-stressed conditions","authors":"Hyuk Sung Yoon , Daisuke Tsugama","doi":"10.1016/j.jplph.2025.154476","DOIUrl":"10.1016/j.jplph.2025.154476","url":null,"abstract":"<div><div>VASCULAR SPECIFICITY FACTOR 1 (VSF-1) is a basic leucine zipper transcription factor identified in tomato (<em>Solanum lycopersicum</em> L.). VSF-1 regulates vascular-specific gene expression and is homologous to an <em>Arabidopsis thaliana</em> mechanical stress regulator, VIP1, but physiological roles for VSF-1 remain unclear. Here, we demonstrate that VSF-1 shuttles between the nucleus and the cytoplasm in response to hypo-osmotic stress. In <em>Arabidopsis</em> plants overexpressing the VSF-1-GFP fusion protein, VSF-1-GFP was mainly detected in the cytoplasm under unstressed conditions but in the nucleus under hypo-osmotically stressed conditions. VSF-1 contains three serine residues within HXRXXS motifs, which can serve as its phosphorylation and 14-3-3 protein-binding sites. In a transient gene expression system in <em>Nicotiana benthamiana</em> leaves, GFP-fused VSF-1 variants where those serine residues were replaced with alanine exhibited nuclear accumulation even under unstressed conditions. GFP-fused VSF-1 variants lacking those HXRXXS motifs also exhibited such nuclear accumulation. The VSF-1 variants lacking those HXRXXS motifs failed to interact with 14-3-3 proteins in a yeast two-hybrid system. These findings suggest that the nuclear accumulation of VSF-1 is triggered by hypo-osmotic stress through its dissociation from 14-3-3 proteins, similar to that of VIP1. The <em>Arabidopsis</em> VSF-1-GFP-overexpressing lines exhibited retarded germination and growth in the presence of mannitol, which can induce hyper-osmotic stress and repress nuclear accumulation of VSF-1. These results are consistent with phenotypes from VIP1-GFP-overexpressing lines in a previous study, indicating a conserved role for VIP1 and VSF-1 in regulating osmotic stress responses.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154476"},"PeriodicalIF":4.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor CsPAT1 from tea plant (Camellia sinensis) is involved in drought tolerance by modulating phenylpropanoid biosynthesis 茶树转录因子CsPAT1通过调节苯丙素的生物合成参与了茶树的抗旱性

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-03-17 DOI: 10.1016/j.jplph.2025.154474

Jing-Wen Li , Ping Zhou , Zhi-Hang Hu , Ai-Sheng Xiong , Xing-Hui Li , Xuan Chen , Jing Zhuang

{"title":"The transcription factor CsPAT1 from tea plant (Camellia sinensis) is involved in drought tolerance by modulating phenylpropanoid biosynthesis","authors":"Jing-Wen Li , Ping Zhou , Zhi-Hang Hu , Ai-Sheng Xiong , Xing-Hui Li , Xuan Chen , Jing Zhuang","doi":"10.1016/j.jplph.2025.154474","DOIUrl":"10.1016/j.jplph.2025.154474","url":null,"abstract":"<div><div>Tea plants, in particular, leafy cash crops, prefer warm and humid climates. Our previous work identified CsPAT1 as a facilitator of lignin biosynthesis in tea plants. The specific role of CsPAT1 in tea plants’ abiotic stress response remains unclear. In this study, we found that the expression of <em>CsPAT1</em> in tea plants was induced under drought, cold, heat, and ABA treatments. <em>CsPAT1</em> transgenic <em>Arabidopsis</em> lines displayed enhanced drought tolerance compared with wild-type (WT) controls. The SOD and POD activities, proline content, and expression levels of drought-responsive genes were significantly increased in transgenic <em>Arabidopsis</em> under drought stress treatment. Transcriptome analysis revealed a significant enrichment of differentially expressed genes (DEGs) in the flavonoid biosynthesis pathway. Correspondingly, total flavonoid contents were significantly higher in the <em>CsPAT1</em> transgenic lines. Through UPLC–MS/MS-based flavonoid metabolome analysis, we identified and quantified 24 flavonoid metabolites. Notably, <em>CsPAT1</em> transgenic lines exhibited significantly lower levels of phenylpropanoids and hydroxycinnamic acids, key precursors in phenylpropanoid biosynthesis. Conversely, nine flavonoid compounds were significantly elevated in the transgenic lines, including apigenin, luteolin 7-<em>O</em>-glucoside, kaempferide, naringenin, butin, catechin, biochanin A, daidzin, and genistein. These findings suggest that CsPAT1 may enhance drought resistance by regulating the phenylpropanoid metabolic pathway. Our results provide insights for future breeding strategies to enhance drought tolerance in tea plants.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154474"},"PeriodicalIF":4.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth-defence carbon allocation is complementary for enhanced crop yield under drought and heat stress in tolerant chickpea genotypes 在干旱和热胁迫下，生长防御碳分配对耐鹰嘴豆基因型作物产量的提高是互补的

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-03-08 DOI: 10.1016/j.jplph.2025.154473

Samson B.M. Chimphango , Dunja MacAlister , John B.O. Ogola , A. Muthama Muasya

{"title":"Growth-defence carbon allocation is complementary for enhanced crop yield under drought and heat stress in tolerant chickpea genotypes","authors":"Samson B.M. Chimphango , Dunja MacAlister , John B.O. Ogola , A. Muthama Muasya","doi":"10.1016/j.jplph.2025.154473","DOIUrl":"10.1016/j.jplph.2025.154473","url":null,"abstract":"<div><div>Non-structural carbohydrates (NSC) are major substrates for primary and secondary plant metabolism with various functions including growth, storage of carbon (C) and energy, osmotic adjustment and synthesis of antioxidants for defence against biotic and abiotic stresses. The allocation of C to growth and defence molecules is labelled antagonistic because it is perceived that limited photosynthates produced under stress is allocated preferentially to defence molecules at the expense of growth, leading to the development of the growth-defence trade-off concept. Several studies and literature reviews have provided evidence both in support and against the growth-defence trade-off. Therefore, it remains unclear whether the allocation of NSC to storage and defence molecules is at the expense of plant growth, especially in annual or short-lived flowering plants. This article reviews literature on sugar and antioxidant metabolism in tolerant/desi and sensitive/kabuli genotypes of chickpea under drought and heat stress conditions. The results show that some of the desi genotypes and drought and heat stress tolerant genotypes accumulated greater NSC, proline or antioxidant enzymes and produced higher biomass and seed yield than kabuli and sensitive genotypes under stress. This is new evidence to support the view that plants accumulate NSC and secondary metabolites and grow at the same time under drought and heat stress conditions which implies complementary allocation of C to growth and defence metabolism. Understanding the growth-defence trade-off and its application is important as it affects plant growth, seed yield, and plant fitness in both natural ecosystems and crop improvement programmes in agriculture.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"307 ","pages":"Article 154473"},"PeriodicalIF":4.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide analysis of SUMO conjugation pathway members in broccoli and the involvement of BoSIZ1 in response to ABA 西兰花SUMO偶联通路成员的全基因组分析及BoSIZ1在ABA应答中的作用

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-03-04 DOI: 10.1016/j.jplph.2025.154472

Sibo Wang, Yukai Ji, Jing Han, Jingsong Guo, Xiaoxue Hu, Wei Ji

{"title":"Genome-wide analysis of SUMO conjugation pathway members in broccoli and the involvement of BoSIZ1 in response to ABA","authors":"Sibo Wang, Yukai Ji, Jing Han, Jingsong Guo, Xiaoxue Hu, Wei Ji","doi":"10.1016/j.jplph.2025.154472","DOIUrl":"10.1016/j.jplph.2025.154472","url":null,"abstract":"<div><div>The small ubiquitin-like protein modifier (SUMO) is a conserved protein that modifies target proteins by attaching to them, changing their functions, localizations, and interactions. However, there is limited knowledge regarding the process of SUMOylation in broccoli (<em>Brassica oleracea</em> var. <em>italica</em>), a highly nutritious vegetable that is widely consumed. In this study, a total of 40 genes including 6 families associated with the SUMOylation pathway were identified in the broccoli genome. Western blot analysis using AtSUMO1 antibody showed that SUMOylation levels increased as broccoli sprouts grew, peaking at 11 days when true leaves were fully developed. RT-qPCR analysis of 10 SUMO pathway genes showed that most of them were upregulated in response to high temperature, NaCl, and abscisic acid (ABA) stimuli within 24 h. Western blot analysis showed changes in SUMOylation dynamics in broccoli sprouts under abiotic stress conditions, regulating SUMOylated proteins. The nuclear localization of the SUMO E3 ligase BoSIZ1a was determined, along with its SUMOylation activity in vivo. Overexpression of <em>BoSIZ1a</em> in <em>Arabidopsis</em> resulted in reduced sensitivity to ABA and decreased expression of ABA-responsive genes (<em>AtABF3</em>, <em>AtADH</em>, <em>AtEm6</em>, <em>AtABI5</em>, <em>AtRAB18</em>, and <em>AtRD29A</em>). Collectively, this study reveals the organization of the broccoli SUMOylation system and highlights the crucial function of SUMOylation in broccoli's response to abiotic stress, as well as the significant contribution of <em>BoSIZ1a</em> in the plant's ABA response.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"307 ","pages":"Article 154472"},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sugar sensors in plants: Orchestrators of growth, stress tolerance, and hormonal crosstalk 植物中的糖传感器：生长、抗逆性和激素串扰的协调者

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-02-28 DOI: 10.1016/j.jplph.2025.154471

Laha Supriya , Deepika Dake , Nyanthanglo Woch , Prodosh Gupta, Kodetham Gopinath, Gudipalli Padmaja, Mehanathan Muthamilarasan

{"title":"Sugar sensors in plants: Orchestrators of growth, stress tolerance, and hormonal crosstalk","authors":"Laha Supriya , Deepika Dake , Nyanthanglo Woch , Prodosh Gupta, Kodetham Gopinath, Gudipalli Padmaja, Mehanathan Muthamilarasan","doi":"10.1016/j.jplph.2025.154471","DOIUrl":"10.1016/j.jplph.2025.154471","url":null,"abstract":"<div><div>Sugars, vital metabolites for cellular health, play a central role in regulating diverse intracellular pathways that control plant growth and development. They also enhance stress responses, enabling plants to endure adverse conditions. A few intracellular molecules involved in sensing the intracellular sugar content and concomitantly facilitating appropriate response (growth or survivability) are known as sugar sensors. Among the numerous sugar sensors identified in plants, this review focuses on four extensively studied sugar sensors, namely hexokinase (HXK), Sucrose non-fermenting 1-related kinase-1 (Snf1-related kinase-1 or SnRK1), Target of rapamycin (TOR), and trehalose 6-phosphate (T6P). This review explores the multifaceted functions of these sugar sensors, highlighting their critical role in balancing energy metabolism and coordinating physiological processes under optimal and adverse conditions. By analyzing their involvement in plant growth, development, and stress response, this review underscores the significance of these sensors throughout the plant life cycle. Furthermore, this review highlights the intricate interplay among these sugar sensors, demonstrating how their activities are finely tuned and interdependent. We also examined the crosstalk between these sugar sensors and phytohormones, fine-tuning plant responses to environmental stimuli. Altogether, this review elucidates the significance of sugar sensors as integrators of metabolic and hormonal signals, providing a comprehensive understanding of their pivotal roles in plant biology. This knowledge paves the way for potential agricultural innovations to enhance crop productivity and resilience in the face of climate change.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"307 ","pages":"Article 154471"},"PeriodicalIF":4.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic biology approaches to improve Rubisco carboxylation efficiency in C3 Plants: Direct and Indirect Strategies 提高C3植物Rubisco羧化效率的合成生物学方法：直接和间接策略

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-02-27 DOI: 10.1016/j.jplph.2025.154470

Chuwen Cui , Mengting Shang , Zhigang Li , Jianwei Xiao

{"title":"Synthetic biology approaches to improve Rubisco carboxylation efficiency in C3 Plants: Direct and Indirect Strategies","authors":"Chuwen Cui , Mengting Shang , Zhigang Li , Jianwei Xiao","doi":"10.1016/j.jplph.2025.154470","DOIUrl":"10.1016/j.jplph.2025.154470","url":null,"abstract":"<div><div>Food security remains a pressing issue due to the growing global population and climate change, including the global warming along with increased atmospheric CO<sub>2</sub> levels, which can negatively impact C<sub>3</sub> crop yields. A major limitation in C<sub>3</sub> plants is the inefficiency of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) due to its low carboxylation activity and competing oxygenase activity. Improving Rubisco efficiency in C<sub>3</sub> plants is thus essential for improving photosynthetic performance. Recent advances in synthetic biology have introduced promising strategies to overcome these limitations. This review highlights the latest synthetic biology and gene transformation techniques aimed at optimizing Rubsico carboxylation efficiency. Next, direct approaches such as engineering Rubisco subunits by replacing plant Rubisco with proteins from other organisms are discussed. Additionally, indirect strategies involve modifications of Rubisco-interacting proteins and adjustment of Rubisco environment. We explore CO<sub>2</sub>-concentrating mechanisms (CCMs) based on pyrenoids and carboxysomes, which increase local CO<sub>2</sub> concentrations around Rubisco thus favouring the carboxylation reaction. Lastly, photorespiratory bypasses are also covered in this review.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"307 ","pages":"Article 154470"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HmGST9, an anthocyanin-related glutathione S-transferase gene, is essential for sepals coloration in Hydrangea macrophylla HmGST9是花青素相关谷胱甘肽s -转移酶基因，在绣球萼片着色中起重要作用

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-02-25 DOI: 10.1016/j.jplph.2025.154466

Haixia Chen, Penghu Lei, Huijun Zhang, Yajing Wang, Xuan Li, Hui Jiang, Jiren Chen

{"title":"HmGST9, an anthocyanin-related glutathione S-transferase gene, is essential for sepals coloration in Hydrangea macrophylla","authors":"Haixia Chen, Penghu Lei, Huijun Zhang, Yajing Wang, Xuan Li, Hui Jiang, Jiren Chen","doi":"10.1016/j.jplph.2025.154466","DOIUrl":"10.1016/j.jplph.2025.154466","url":null,"abstract":"<div><div>Flower color is an important ornamental trait of <em>Hydrangea macrophylla</em>. The transport of anthocyanin from endoplasmic reticulum to vacuole for storage is the basis of flower color formation. Glutathione S-transferase (GST) plays an important role in this transport process. However, little is known about the <em>GST</em> genes involved in anthocyanin transport and their functions in <em>H. macrophylla</em>. In this study, the sepals of <em>H. macrophylla</em> ‘Blue Mom’ was used as the experimental material. The gene <em>HmGST9</em> that may be involved in anthocyanin accumulation was identified from the genome, and it was found to be located in the endoplasmic reticulum and tonoplast. Through <em>Arabidopsis tt19</em> mutant molecular complementation experiment, it was proved that <em>HmGST9</em> could restore anthocyanin accumulation in vegetative tissues of <em>Arabidopsis tt19</em> mutant, but could not restore the color of seed coat. In the sepals of <em>H. macrophylla</em>, virus-induced <em>HmGST9</em> gene silencing resulted in a significant decrease in anthocyanin content, and the structural genes and transcription factors in the anthocyanin biosynthesis pathway were also significantly down-regulated. In contrast, transient overexpression of <em>HmGST9</em> in sepals resulted in a significant increase in anthocyanin content and promoted the up-regulation of related structural genes and transport genes. These results indicate that <em>HmGST9</em> plays an important role in regulating the transport and accumulation of anthocyanins in <em>H. macrophylla</em>, and is of great significance for analyzing the molecular mechanism of flower color formation in <em>H. macrophylla</em>.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"307 ","pages":"Article 154466"},"PeriodicalIF":4.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of the potassium transporter TaHAK18 on wheat growth and potassium uptake under stressful K+ conditions 钾转运体TaHAK18对钾胁迫条件下小麦生长和钾吸收的影响

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-02-22 DOI: 10.1016/j.jplph.2025.154459

Tengfei Liu , Yanan Zhang , Yumin Xie , Ruipeng Yang , Mengying Yuan , Yanke Li , Haixia Xu , Xinli Zhu , Tengzhao Song , Xiyong Cheng

{"title":"Impact of the potassium transporter TaHAK18 on wheat growth and potassium uptake under stressful K+ conditions","authors":"Tengfei Liu , Yanan Zhang , Yumin Xie , Ruipeng Yang , Mengying Yuan , Yanke Li , Haixia Xu , Xinli Zhu , Tengzhao Song , Xiyong Cheng","doi":"10.1016/j.jplph.2025.154459","DOIUrl":"10.1016/j.jplph.2025.154459","url":null,"abstract":"<div><div>Potassium (K), an indispensable nutrient for plant growth and development, plays a crucial role in plant stress resistance. Within the K<sup>+</sup> regulatory network in plants, the HAK/KUP/KT gene family comprises a dominant group of K<sup>+</sup> transport proteins responsible for K<sup>+</sup> uptake and transport. This study functionally characterized the wheat gene <em>TaHAK18</em>, which encodes a putative K<sup>+</sup> transporter. Plasma membrane-localized TaHAK18 was significantly upregulated under low-K<sup>+</sup> conditions and showed tissue-specific expression, being most abundant in leaves. A functional analysis in yeast demonstrated that TaHAK18 complements K<sup>+</sup>-uptake deficiencies, confirming its role in K<sup>+</sup> transport. <em>Arabidopsis</em> plants overexpressing <em>TaHAK18</em> experienced enhanced growth under both low- and normal-K<sup>+</sup> conditions, with greater fresh weight, lateral root formation, and primary root length. Barley stripe mosaic virus-mediated gene silencing in wheat revealed that TaHAK18 is instrumental for K<sup>+</sup> accumulation and plant growth under low-K<sup>+</sup> stress. <em>TaHAK18</em> has the capacity to enhance the growth and the accumulation of K<sup>+</sup> in transgenic rice plants. These results indicated that TaHAK18 is a key regulator of K<sup>+</sup> uptake and homeostasis in wheat, with potential implications for improving plant tolerance to low-K<sup>+</sup> stress.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"307 ","pages":"Article 154459"},"PeriodicalIF":4.0,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of plant growth-promoting rhizobacteria in sustainable agriculture: Addressing environmental and biological challenges 植物生长促进根瘤菌在可持续农业中的作用：应对环境和生物挑战

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-02-19 DOI: 10.1016/j.jplph.2025.154455

Abdul Wahab , Farwa Batool , Gholamreza Abdi , Murad Muhammad , Shahid Ullah , Wajid Zaman

{"title":"Role of plant growth-promoting rhizobacteria in sustainable agriculture: Addressing environmental and biological challenges","authors":"Abdul Wahab , Farwa Batool , Gholamreza Abdi , Murad Muhammad , Shahid Ullah , Wajid Zaman","doi":"10.1016/j.jplph.2025.154455","DOIUrl":"10.1016/j.jplph.2025.154455","url":null,"abstract":"<div><div>This review underscores the importance of plant growth-promoting rhizobacteria (PGPR), fostering sustainability to address various environmental and biological issues. PGPR helps crops withstand salinity, nutrient deficiencies, and drought stress while tackling agricultural threats. Sustainable agriculture has emerged as a response to the social and economic problems farming practices face. Plants encounter obstacles from biotic stressors such as bacteria, viruses, nematodes, arachnids, and weeds that impede their growth. Furthermore, PGPR enhances plant growth through improved nutrient absorption and defense against pests. <em>Bacillus subtilis</em> utilizes indirect methods to combat diseases and protect plants from various diseases and pests. Additionally, PGPR acts as a bio-fertilizer that mitigates drought stress effects on crops in various regions worldwide. This review proposes strategies to boost productivity and improve bio-inoculant efficiency under real-world conditions. PGPR demonstrates its role in combating threats by influencing plant defense mechanisms, initiating systemic resistance responses, and regulating gene expression related to pathogen detection and defense signaling pathways. It maintains a balanced root microbiome by suppressing harmful microbial proliferation while promoting beneficial microbial interactions. Despite the challenges posed by technology and ethical considerations surrounding their modification, integrating PGPR into farming methods holds promise for sustainable agriculture. Given the increasing impact of climate change, PGPR plays a crucial role in improving crop resilience, enhancing soil quality, and reducing dependence on synthetic agricultural inputs.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"307 ","pages":"Article 154455"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143529804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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