Journal of plant physiology最新文献

{"title":"Evidence for a JA-responsive SNAC1–ASMT1 regulatory module contributing to melatonin-mediated salinity stress tolerance in Barley","authors":"Roohollah Shamloo-Dashtpagerdi ,&nbsp;Hadi Pirasteh-Anoshe ,&nbsp;Sirous Tahmasebi","doi":"10.1016/j.jplph.2025.154587","DOIUrl":"10.1016/j.jplph.2025.154587","url":null,"abstract":"<div><div>Melatonin has emerged as a crucial mediator in plant responses to abiotic stresses, with its regulatory effects closely dependent on its endogenous levels and biosynthetic dynamics. However, in barley (<em>Hordeum vulgare</em> L.), the upstream regulatory mechanisms of melatonin biosynthesis under salinity stress, and their connection to hormonal signaling, remain largely unknown. In this study, we explore the potential regulatory modules of the key melatonin biosynthesis gene, <em>Acetylserotonin O-Methyltransferase 1</em> (<em>ASMT1</em>), in barley. Promoter analysis identified putative Stress-responsive NAC1 (SNAC1) binding motifs within the <em>ASMT1</em> promoter region, and transcriptomic data showed the differential expression of <em>SNAC1</em> and <em>ASMT1</em> in response to salinity exposure. To further investigate this regulatory relationship, we performed a controlled greenhouse experiment with six treatments: control, Jasmonic acid (JA), DIECA (a JA biosynthesis inhibitor), salinity (S), JA + S, and DIECA + S. Exogenous JA significantly increased <em>SNAC1</em> and <em>ASMT1</em> expression, boosted melatonin levels, activated antioxidant enzymes (SOD, CAT, APX), and reduced oxidative damage and photosynthetic decline under salinity. In contrast, inhibition of JA biosynthesis by DIECA attenuated these responses, supporting the involvement of JA signaling in this pathway. Additionally, we observed a statistically significant correlation between gene expression profiles and melatonin content. While further functional validation is needed, our results support a model in which JA signaling contributes to salinity-induced melatonin biosynthesis, possibly through the <em>SNAC1–ASMT1</em> axis. These findings offer new insights into how hormones regulate melatonin during stress and provide a framework for future functional studies aimed at improving stress tolerance in barley.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"313 ","pages":"Article 154587"},"PeriodicalIF":4.1,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osmotic stress-induced CsRCI2E endosomal trafficking modulates CsPIP2 aquaporins at the plasma membrane in Camelina sativa","authors":"Jung-Ho Shin ,&nbsp;Hyun-Sung Kim ,&nbsp;Sehee Kim ,&nbsp;Won Park ,&nbsp;Sung-Ju Ahn","doi":"10.1016/j.jplph.2025.154586","DOIUrl":"10.1016/j.jplph.2025.154586","url":null,"abstract":"<div><div>Rare Cold Inducible 2s (RCI2s) are membrane-associated proteolipids dynamically trafficking between the plasma membrane (PM) and the endomembrane system. Their expression is upregulated in response to abiotic stresses, including cold, heat, drought, and salinity, contributing to plant stress tolerance. CsRCI2E interacts with the water transport protein CsPIP2; 1, reducing its abundance at the PM under NaCl-induced stress. Consequently, CsRCI2E is considered a potential regulator of CsPIP2 endocytosis involved in maintaining cellular homeostasis. However, its precise role in membrane trafficking remains unclear. Therefore, this study aims to investigate the rapid internalization of CsRCI2E and CsPIP2 under mannitol-induced and NaCl-induced osmotic stress using a sucrose density gradient. <em>CsRCI2E</em> transcription levels increased significantly 3 h posttreatment with mannitol or NaCl. <em>CsRCI2E</em> overexpression enhanced stress tolerance and reduced reactive oxygen species accumulation-induced cellular damage during <em>Camelina</em> germination. Despite no concurrent change in <em>CsRCI2E</em> gene expression, the subcellular distribution of CsRCI2E and CsPIP2s (CsPIP2; 1 and CsPIP2; 2) shifted rapidly from the PM to the endomembrane within 0.5 h following osmotic stress. Additionally, <em>CsRCI2E</em> overexpression induced internalization and subcellular redistribution of CsRCI2E and CsPIP2s under osmotic stress and non-stress conditions. These findings suggest that CsRCI2E internalization functions as a sensing mechanism during the initial phase of osmotic shocks. Furthermore, elevated CsRCI2E levels promote CsPIP2s membrane trafficking from the PM to the endomembrane system, supporting water homeostasis in <em>Camelina</em>.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"313 ","pages":"Article 154586"},"PeriodicalIF":4.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GmAIR12-5 governs soybean nodule development associated with phosphorus availability","authors":"Yuxiu Qin ,&nbsp;Huaikang Ruan ,&nbsp;Kang Chen ,&nbsp;Xing Lu ,&nbsp;Tianqi Wang ,&nbsp;Jiang Tian ,&nbsp;Cuiyue Liang","doi":"10.1016/j.jplph.2025.154585","DOIUrl":"10.1016/j.jplph.2025.154585","url":null,"abstract":"<div><div>Low phosphorus (P) bioavailability limits nitrogen (N) fixation in legume nodules. Although auxin-induced root 12 (AIR12) contributes to plant stress resistance, its role in regulating nodule adaptation to P deficiency remains elusive. In this study, a hydroponic experiment revealed that P deficiency restricted soybean (<em>Glycine max</em>) growth and decreased nodule development, especially in big nodules (diameter≥2 mm). Following analyzing the phylogenetic relationship and expression pattern, we identified that <em>GmAIR12-5</em> might be highly expressed in nodules and associated with nodule development. Overexpression of <em>GmAIR12-5</em> led to significant increases in plant growth and acquisition of N and P in soybean, particularly under low-P conditions. Conversely, suppression of <em>GmAIR12-5</em> reduced the plant growth and nutrients absorption of soybean. Significantly, under low-P levels, overexpression of <em>GmAIR12-5</em> increased the number and weight of big nodules by 67.7 % and 67.4 %, respectively, while nodule development was inhibited by <em>GmAIR12-5</em> suppression. In contrast, under high-P conditions, <em>GmAIR12-5</em> mutants only exhibited significant alterations in root architecture and nodule weight, while maintaining comparable shoot biomass and nodule number to wild type. Furthermore, the overexpression of <em>GmAIR12-5</em> significantly down-regulated the superoxide anion content and enhanced the number of infected cells under low P conditions. These results demonstrate that <em>GmAIR12-5</em> contributes to nodule development by avoiding reactive oxygen species accumulation. This finding enhances our understanding of the role of AIR12 in legume crops.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"313 ","pages":"Article 154585"},"PeriodicalIF":4.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic modelling: Insights into the machine room of plant metabolism","authors":"Tiago M. Machado,&nbsp;Nadine Töpfer,&nbsp;Fatemeh Soltani","doi":"10.1016/j.jplph.2025.154584","DOIUrl":"10.1016/j.jplph.2025.154584","url":null,"abstract":"<div><div>Plant growth, development, and environmental interactions is enabled through the coordinated activity of numerous biochemical reactions that constitute plant metabolic networks. The inherent complexity and interconnectivity within these networks underscore the importance of investigating plant metabolism from a network perspective. Metabolic modelling provides a holistic <em>in silico</em> representation of plant metabolism, enabling mechanistic insights into network-level processes. In this review, we consolidate recent trends in plant metabolic modelling, highlighting how these approaches can be exploited to study metabolism from subcellular to community and ecosystem levels. We discuss how the scope of plant metabolic modelling has broadened to represent diverse plant species, genotype- and context-specific metabolism as well as specialized metabolic pathways, and to capture spatiotemporal resolution and plant-microbe interactions. Moreover, we review machine learning and deep learning frameworks that assist model reconstruction, parameterization, and analysis, explore hybrid strategies that enhance mechanistic models, and address current challenges and future directions in the field of plant metabolic modelling.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"314 ","pages":"Article 154584"},"PeriodicalIF":4.1,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the role of the plasma membrane H+-ATPase in plant adaptation to phosphorus deficiency in rice under various nitrogen sources and rhizosphere pH","authors":"Maoxing Zhang ,&nbsp;Mengru Xie ,&nbsp;Ming Ding ,&nbsp;Liang Xiao ,&nbsp;Min Yu ,&nbsp;Lars H. Wegner ,&nbsp;Sergey Shabala ,&nbsp;Ting Pan ,&nbsp;Yiyong Zhu","doi":"10.1016/j.jplph.2025.154582","DOIUrl":"10.1016/j.jplph.2025.154582","url":null,"abstract":"<div><div>Soil pH is critical for the bioavailability of nutrients and their consequent uptake by plant roots. This is specifically true for N and P, two key macronutrients that are essential for all aspects of plant growth and development. Importantly, availability of one nutrient can affect acquisition and translocation of another, although the mechanistic basis of this process remains unexplored. In this work, we combined a physiological (growth; ionomics), molecular (RNAseq and qPCR), biochemical (enzymatic assays) and genetic (using gain-of-function mutants) approaches to investigate the effect of interplay between P availability, two forms of N supply (NO₃⁻ vs NH₄⁺) and rhizosphere pH (3.0 vs 6.5) on rice plants. In general, rice plants grown in the presence of NH₄⁺ performed better than those treated with NO₃⁻ and better at pH 6.5 than at pH 3. P deprivation significantly reduced N accumulation in leaves but increased N in roots under both NH₄⁺ and NO₃⁻ treatments. Transcriptome analysis revealed 8749 differently expressed genes (DEGs) in leaves and 6519 DEGs in roots under P deprivation at pH 6.5, related to membrane function, cellular response, metabolism, and cell signaling. Among the DEGs, the plasma membrane H⁺-ATPase genes were significantly induced by both P deprivation under NO₃⁻ and NH₄⁺ treatments, indicating a possible role of H⁺-ATPase in plant adaptive responses to P nutrition. The latter was confirmed in direct experiments combining ³³P radiotracers. Overexpression of <em>OSA1</em> encoding a H⁺-ATPase improved nutrient uptake and rice growth. Overall, these results suggest that PM H⁺-ATPase plays a crucial role in the regulation of N and P uptake and provide a new approach to develop crop varieties that are more efficient at absorbing and utilizing nutrients and, hence, capable to achieve optimal yields.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154582"},"PeriodicalIF":4.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of MADS-box genes in Chinese cherry and overexpression of CpMADS2 promoting early flowering in transgenic Arabidopsis","authors":"Yaan Wen,&nbsp;Qiandong Hou,&nbsp;Fali Chen,&nbsp;Chunqiong Shang,&nbsp;Yuxin Leng,&nbsp;Guang Qiao","doi":"10.1016/j.jplph.2025.154583","DOIUrl":"10.1016/j.jplph.2025.154583","url":null,"abstract":"<div><div>The Chinese cherry (<em>Cerasus pseudocerasus</em> Lindl.) cv. 'Manaohong', a distinctive cultivar indigenous to Guizhou Province, China, possesses significant nutritional and economic value. In our previous studies, we observed an abnormal flowering phenomenon in the 'Manaohong' cherry. The MADS-box gene family plays a pivotal role in regulating flowering time and the floral organs development in plants. In this study, we identified 51 <em>CpMADS</em> genes. Transcriptome analysis revealed that the <em>PI-like</em> gene <em>CpMADS2</em> was significantly differentially expressed between normal flowers (NF) and abnormal flowers (AF) in Chinese cherry. To further investigate the functional role of <em>CpMADS2</em>, it was cloned from the Chinese cherry. Overexpression of <em>CpMADS2</em> in <em>Arabidopsis thaliana</em> exhibited earlier flowering compared to wild-type. Yeast two-hybrid (Y2H) assay and luciferase complementation assay (LCA) confirmed that CpMADS2 interacts with the CpFT, suggesting that CpMADS2 may promotes flowering by interacting with CpFT. This study provides a theoretical foundation for further research on the role of <em>CpMADS2</em> in regulating abnormal flowering in Chinese cherry.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154583"},"PeriodicalIF":4.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-physics approach to probing plant responses: From calcium signaling to thigmonastic motion","authors":"Sabrina Gennis ,&nbsp;Matthew D. Biviano ,&nbsp;Kristoffer P. Lyngbirk ,&nbsp;Hannah R. Thomas ,&nbsp;Viktoriya Vasina ,&nbsp;Christine Faulkner ,&nbsp;Michael Knoblauch ,&nbsp;Kaare H. Jensen","doi":"10.1016/j.jplph.2025.154570","DOIUrl":"10.1016/j.jplph.2025.154570","url":null,"abstract":"<div><div>Plants respond to biotic and abiotic stresses through complex and dynamic mechanisms that integrate physical, chemical, and biological cues. Here, we present a multi-physics platform designed to systematically investigate these responses across scales. The platform combines a six-axis micromanipulator with interchangeable probes to deliver precise mechanical, electrostatic, optical, and chemical stimuli. Using this system, we explore calcium signaling in <em>Arabidopsis thaliana</em>, thigmonastic motion in <em>Mimosa pudica</em>, and chemical exchange via microinjection in <em>Rosmarinus officinalis L.</em> and <em>Ocimum basilicum</em>. Our findings highlight stimulus-specific and spatially dependent responses: mechanical and electrostatic stimuli elicit distinct calcium signaling patterns, while repeated electrostatic stimulation exhibited evidence of response fatigue. Thigmonastic responses in <em>Mimosa pudica</em> depend on the location of perturbation, highlighting the intricate bi-directional calcium signaling. Microinjection experiments successfully demonstrate targeted chemical perturbations in glandular trichomes, opening avenues for biochemical studies. This open-source platform provides a versatile tool for dissecting plant stress responses, bridging the gap between fundamental research and applied technologies in agriculture and bioengineering. By enabling precise, scalable, and reproducible studies of plant-environment interactions, this work offers new insights into the mechanisms underlying plant resilience and adaptability.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154570"},"PeriodicalIF":4.1,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SPOTLIGHT: Nitrate transporter come ABA receptor: the beguiling role of NRT1.1B in rice","authors":"Takuya Yoshida ,&nbsp;Alisdair R. Fernie","doi":"10.1016/j.jplph.2025.154579","DOIUrl":"10.1016/j.jplph.2025.154579","url":null,"abstract":"","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154579"},"PeriodicalIF":4.1,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spotlight: Evolution of Saline-tolerant Arabidopsis in the Cape Verde Islands","authors":"Alisdair R. Fernie","doi":"10.1016/j.jplph.2025.154580","DOIUrl":"10.1016/j.jplph.2025.154580","url":null,"abstract":"","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154580"},"PeriodicalIF":4.1,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autoimmunity and hyperactivation of immune responses by HAE-FLS2 chimera","authors":"Tian Zhao ,&nbsp;Yingying Wu ,&nbsp;Ahmed ElGamal ,&nbsp;Ruihan Zhang ,&nbsp;Shuying Yang ,&nbsp;Yiji Xia ,&nbsp;Weiwen Kong ,&nbsp;Jinglan Liu ,&nbsp;M.E.A. Aborehab ,&nbsp;Yiping Wang","doi":"10.1016/j.jplph.2025.154581","DOIUrl":"10.1016/j.jplph.2025.154581","url":null,"abstract":"<div><div>Plants employ cell surface receptors to perceive extracellular signals and initiate appropriate cellular responses, thereby regulating diverse physiological processes. The well-characterized leucine-rich repeat receptor-like kinases (LRR-RLK) type receptor FLAGELLIN-SENSITIVE 2 (FLS2) functions as a pattern recognition receptor (PRR) that specifically detects bacterial flagellin, activating downstream responses including MAPK signaling and ROS burst. Here, we report that engineered expression of a chimeric receptor combining the N-terminal extracellular and transmembrane domains of HAE with the cytosolic kinase domain of FLS2 (HAE-FLS2) leads to excessive activation of plant immune responses, as evidenced by dwarfism, enhanced flg22-induced ROS burst and MAPK activation, and upregulated expression of defense-related genes in transgenic plants. The chimeric transgenic plants exhibit enhanced disease resistance to bacterial and fungal pathogens. Notably, while the introduction of a kinase-dead mutation (D997N) in the FLS2 domain partially attenuated these immune responses, the persistence of the autoimmune phenotype suggests the existence of both D997-associated kinase activity-dependent and -independent signaling mechanisms. Genetic analysis revealed that HAE-FLS2-mediated immunity predominantly depends on BAK1/BKK1, as evidenced by substantial suppression of the autoimmune phenotype in the <em>bak1-5 bkk1</em> mutant background. These findings establish the fundamental basis for elucidating FLS2 activation mechanisms and provide a conceptual framework for engineering plant disease resistance toward different types of pathogens through strategic manipulation of RLK.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154581"},"PeriodicalIF":4.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144828191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}