Journal of plant physiology最新文献

{"title":"Regulation of iron homeostasis by IMA1 and bHLH104 under phosphate starvation in Arabidopsis","authors":"Lu Zheng ,&nbsp;Xiangxiang Meng ,&nbsp;Wenfeng Li ,&nbsp;Yinglong Chen ,&nbsp;Renfang Shen ,&nbsp;Ping Lan","doi":"10.1016/j.jplph.2025.154445","DOIUrl":"10.1016/j.jplph.2025.154445","url":null,"abstract":"<div><div>Phosphate (Pi) starvation disrupts iron (Fe) nutrition at phenotypic, physiological, and transcriptional levels. The alteration of Fe homeostasis plays an important role in the adaptive response to Pi starvation. However, utilizing the antagonistic mechanism between P and Fe nutrition to improve adaptation to Pi deficiency in plants still needs to be explored. Here, we constructed inducible and constitutive expression of Fe regulators <em>IMA1</em> and <em>bHLH104</em>, driven by the <em>CaMV 35S</em> promoter and the promoters of Pi-starvation responsive genes (<em>proIPS1</em> and <em>proPHT1;4</em>), respectively. The Fe regulators <em>bHLH104</em> and <em>IMA1</em> were successfully upregulated in a constitutive and inducible manner under Pi deficiency in these transgenic plants. Regardless of Pi condition, upregulation of <em>bHLH104</em> and <em>IMA1</em> had no significant influence on primary root length or root Fe distribution. Nevertheless, the upregulation of <em>bHLH104</em> and <em>IMA1</em> induced Fe accumulation in the shoots of transgenic plants, particularly under Pi deficiency. Correspondingly, shoot chlorophyll content increased under Fe deficiency in the transgenic plants. In addition, <em>in situ</em> Fe^III distribution revealed that <em>bHLH104</em> and <em>IMA1</em> likely interfere with Fe distribution through different pathways. The inducible upregulation of <em>IMA1</em> significantly led to shoot zinc (Zn) accumulation under Pi deficiency, while the inducible upregulation of <em>bHLH104</em> resulted in a decrease in shoot Zn and manganese (Mn) contents. The enhancement of Fe and Zn accumulation under the inducible expression of <em>IMA1</em> under Pi deficiency was attributed to the induction of high expression of key Fe-uptake genes <em>FRO2</em> and <em>IRT1</em>. The expression of the Zn and Mn uptake genes was also affected in these transgenic plants, which correlated with the changes in Zn and Mn contents. Overall, <em>IMA1</em> is an excellent candidate for enhancing plant Fe and Zn accumulation and can be specifically induced under conditions of Pi deficiency.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154445"},"PeriodicalIF":4.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and biochemical analyses of germination of cowpea (Vigna unguiculata L.) seeds inhibited by n-propyl gallate reveal a key role of alternative oxidase in germination Re-establishment","authors":"Lyndefania Melo de Sousa ,&nbsp;Thais Andrade Germano ,&nbsp;Shahid Aziz ,&nbsp;Matheus Finger Ramos de Oliveira ,&nbsp;Giovanna Magalhães Bastos Salvador ,&nbsp;Rafael de Souza Miranda ,&nbsp;Birgit Arnholdt-Schmitt ,&nbsp;Jose Helio Costa","doi":"10.1016/j.jplph.2025.154446","DOIUrl":"10.1016/j.jplph.2025.154446","url":null,"abstract":"<div><div><em>n-Propyl gallate</em> (PG) is a phenolic compound that influences enzymatic processes, mostly involving AOX, PTOX, LOX, POD, and PPO. Here, analyses of different PG concentrations (1, 2.5, and 5 mM) during cowpea seed germination at 16, 32, and 48h showed that 2.5 mM PG partially inhibited seed germination at 16 and/or 32h, but by 48h the germination re-established. Thus, this PG concentration was chosen to study the molecular and biochemical mechanisms linked to the PG inhibitory effects and germination recovery. PG inhibition was related to lower H₂O₂, higher antioxidant activity, and downregulation of genes linked to cell cycle progression, energy status, and the Krebs cycle at 16 and/or 32h, but these changes were reversed at 48h. In general, genes associated with detoxification, germination-related phytohormones, and NAD(P)H metabolism were highly up-regulated across the time points. <em>AOX1</em> and <em>Pgb1</em> were continuously up-regulated along the time points, and linked to <em>NR</em> transcript level increase only at 48h. These findings indicated that AOX and the phytoglobin cycle, both systems involved in NO levels regulation, worked efficiently in germination re-establishment. However, genes other than AOX associated with potential target enzymes of PG, such as LOX, POD, PTOX and PPO (except at 48h), were mostly unchanged or down-regulated. Genes linked to glycolysis (<em>PFK</em> and <em>PK</em>) and acetate synthesis (<em>PDC</em> and <em>ALDH</em>) connected with AOX via NAD(P)+ were up-regulated under PG mainly at 48h. The data are discussed in light of AOX's role in cell reprogramming to reverse PG-induced inhibition of germination in cowpea seeds.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154446"},"PeriodicalIF":4.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of OSCAs in wheat and over-expression of TaOSCA14D increased salt stress tolerance","authors":"Ruiping Su,&nbsp;Yuning Wang,&nbsp;Ping Cui,&nbsp;Geng Tian,&nbsp;Yuxiang Qin","doi":"10.1016/j.jplph.2025.154449","DOIUrl":"10.1016/j.jplph.2025.154449","url":null,"abstract":"<div><div>Salt stress is a major environmental factor that limits plant growth and productivity. In the early stage of salt stress, the intracellular Ca²⁺ concentration elevates, thereby triggering osmotic stress tolerance signaling pathway. <em>OSCAs</em> encode hyperosmotic gated calcium channels and function as osmotic sensors in <em>Arabidopsis</em>. But the functions of <em>OSCAs</em> in wheat responding to salt stress have not been elucidated. In this study, we identified 42 <em>TaOSCAs</em> and examined their expression pattern in 12 tissues and under salt stress. Further, the salt inducible <em>TaOSCA14D</em> was selected for functional study in response to salt stress. <em>TaOSCA14D</em> was induced by NaCl, PEG, exogenous ABA treatment. Over-expression of <em>TaOSCA14D</em> in <em>Arabidopsis</em> and wheat increased salt stress tolerance. Salt stress related marker genes <em>SnRK2s</em>, <em>ABFs</em>, <em>RD29B</em> were higher expressed in <em>TaOSCA14D</em> transgenic plants than in the wild type under NaCl treatment. Furthermore, the soluble sugar and proline content were higher in transgenic plants than in wild-type ones. Over-expression of <em>TaOSCA14D</em> promoted flowering, decreased spike length and the grain number of per spike. All these results shed some light on the function of <em>OSCAs</em> in tolerance to salt stress and its roles in agronomic trait in wheat.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154449"},"PeriodicalIF":4.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual role of cytosolic GSH in the ABA signaling pathway and plasma membrane ion channel regulation in guard cells of Vicia faba","authors":"Huifei Yin,&nbsp;Toshiyuki Nakamura,&nbsp;Yoshimasa Nakamura,&nbsp;Shintaro Munemasa,&nbsp;Yoshiyuki Murata","doi":"10.1016/j.jplph.2025.154447","DOIUrl":"10.1016/j.jplph.2025.154447","url":null,"abstract":"<div><div>Abscisic acid (ABA) induces stomatal closure in higher plants under drought stress. Glutathione (GSH) negatively regulates ABA-induced stomatal closure and reactive carbonyl species (RCS) play a role as signal mediators downstream of reactive oxygen species production in ABA signaling pathway in <em>Arabidopsis thaliana</em>. Activation of slow (S-type) anion channels and inhibition of inward-rectifying potassium ion (K_in⁺) channels in the plasma membrane are essential for ABA-induced stomatal closure. However, there is limited evidence regarding role of GSH in the activation of S-type anion channels and the inhibition of K_in⁺ channels. We used <em>Vicia faba</em> to clarify the regulation of these ion channels by GSH and RCS. Pretreatment of guard-cell protoplasts with the GSH-supplementing agent, glutathione monoethyl ester (GSHmee), suppressed the activation of S-type anion channels and the inactivation of K_in⁺ channels induced by ABA. The pretreatment with the RCS scavenger carnosine suppressed the activation of S-type anion channels and the inactivation of K_in⁺ channels by ABA. On patch clamping guard-cell protoplasts, the addition of GSH to the pipette (cytosolic) buffer decreased the S-type anion currents and increased the K_in⁺ currents. These results suggest that cytosolic GSH is involved in ABA-induced stomatal closure <em>via</em> negative regulation of ABA signaling and <em>via</em> direct regulation of ion channel activities in <em>V. faba</em>.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154447"},"PeriodicalIF":4.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive influence of selenium on the modulation of ascorbate-glutathione cycle in salt stressed Setaria italica L","authors":"Seerat Saleem ,&nbsp;Naveed Ul Mushtaq ,&nbsp;Inayatullah Tahir ,&nbsp;Chandra Shekhar Seth ,&nbsp;Reiaz Ul Rehman","doi":"10.1016/j.jplph.2025.154448","DOIUrl":"10.1016/j.jplph.2025.154448","url":null,"abstract":"<div><div>Soil salinity is a significant abiotic factor affecting crop yield and global distribution, hence selecting salt-tolerant crop species is crucial for food security. Foxtail millet is a resilient crop suitable for hilly, salinity, and drought-prone areas due to its ability to withstand environmental stressors. In this study, foxtail millet was subjected to high NaCl concentrations (150 mM and 200 mM) and selenium (1 μM, 5 μM, and 10 μM) as a stress mitigator. Increased salinity in foxtail plants hampered the growth with decreased pigment levels, increased H₂O₂ levels (153.6%), lipid peroxidation (32.1%), and electrolyte leakage (155.5%). The application of 1 μM Se positively influenced the root-to-shoot ratio (R) (59.2%), photosynthetic pigments, phenolic content (25.1%), flavonoid content (7%) and hence the antioxidant potential of the salt stressed plants there by decreasing the H₂O₂ levels (26.8%) and suggesting a greater ability to scavenge radicals. Both NaCl and Se induced the AsA-GSH pathway. Se supplementation significantly improved AsA-GSH pathway components such as AsA/DHA (40.8%) and GSH/GSSG ratios (39.6%) in salt-stressed foxtail millet, reducing oxidative stress and efficiently neutralizing H₂O₂. Gene expression validation confirmed that <em>SiAPX</em>, <em>SiDHAR</em>, <em>SiMDHAR</em>, and <em>SiGR</em> showed significant upregulation with 1 μM Se application in salt-stressed foxtail millet plants.</div><div>However, higher Se concentrations (5 μM and 10 μM) led to a reduced fresh weight along with R, increased the MDA and H₂O₂ levels, and did not positively contribute to osmolyte accumulation or improve the AsA/DHA and GSH/GSSG ratios. Elevated Se levels also led to a decreased antioxidant potential. Among the enzymes of the AsA-GSH cycle, higher Se concentrations negatively affected APX, DHAR, MDHAR, and GR activities, indicating stress aggravation rather than mitigation at elevated doses.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154448"},"PeriodicalIF":4.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Rht1 for plant height reduction in two wheat mutants and the effects on yield components","authors":"Xinshe Zhou ,&nbsp;Qingguo Wang ,&nbsp;Huijun Guo ,&nbsp;Yongdun Xie ,&nbsp;Linshu Zhao ,&nbsp;Jiayu Gu ,&nbsp;Huiyuan Li ,&nbsp;Shirong Zhao ,&nbsp;Yuping Ding ,&nbsp;Jie Guo ,&nbsp;Hongchun Xiong ,&nbsp;Luxiang Liu","doi":"10.1016/j.jplph.2025.154420","DOIUrl":"10.1016/j.jplph.2025.154420","url":null,"abstract":"<div><div>Plant height determines lodging resistance and is closely linked to yield stability in wheat. In this study, we identified two semi-dwarf wheat mutants, designated <em>je0370</em> and <em>je0344</em>, using the winter wheat cultivar Jing411 as the wild type (WT). Field experiments revealed that the plant height of these two mutants was significantly lower than that of the WT. In contrast, the thousand-grain weight was significantly higher in <em>je0370</em> but lower in <em>je0344</em> compared to the WT. Bulk Segregant Analysis (BSA) based on exome capture sequencing indicated that the gene responsible for height reduction is located on chromosome 4B. Further genetic linkage analysis mapped the dwarf gene to the interval of 29.26–48.61 Mb on chromosome 4B, corresponding to a genetic distance of 10.79 cM. This region encompasses the <em>Rht1</em> gene; we subsequently sequenced the <em>Rht1</em> gene in <em>je0370</em> and <em>je0344</em> and identified a C-T mutation at position 190 bp, resulting in a truncation of the DELLA domain in both mutants. Further analysis using Cleaved Amplified Polymorphic Sequences (CAPS) markers in F₂ populations demonstrated that plants with homozygous <em>Rht1</em> mutations exhibited significantly reduced plant height and thousand-grain weight, while heterozygous plants displayed intermediate effects. However, the mutation did not significantly affect spikelet number, effective spike number, or spike length. These findings conclusively demonstrate that the <em>Rht1</em> mutation is responsible for plant dwarfism and reduced grain weight, without substantial impacts on other yield components. This study provides invaluable insights into the utilization of <em>Rht1</em> in wheat breeding.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154420"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The difference of organophosphate esters (OPEs) uptake, translocation and accumulation mechanism between four varieties in Poaceae","authors":"Wenxuan Wang,&nbsp;Haiou Wang,&nbsp;Xiaoyu Ren","doi":"10.1016/j.jplph.2024.154418","DOIUrl":"10.1016/j.jplph.2024.154418","url":null,"abstract":"<div><div>To explore variation patterns of uptake, translocation, and accumulation processes responding to organophosphate esters (OPEs) among Poaceae plants, hydroponic and computer simulation experiments were executed. Plant growth, OPEs’ concentration, and bioinformation and transcript of lipid transporters in the three terrestrial barley, wheat, and maize and aquatic rice seedlings were studied after exposure to seven OPE congeners. Four types of plants could accumulate seven OPE congeners. OPEs could promote rice growth by upregulating <em>IAA27</em> hormone gene. However, maize growth was inhibited due to upregulating <em>IAA17</em> hormone gene. In general, OPEs with log <em>K</em>_<em>ow</em> &gt; 4 tended to accumulate in roots of the four types of plants. Furthermore, the uptake, translocation, and accumulation mechanism of OPEs in different plants showed species-specific, depending on chemical properties of OPEs and biological factors specifically referring to the binding ability and gene expression of lipid transporters. The uptake and accumulation of OPE in aquatic rice roots were mainly influenced by biological factors. On the contrary, terrestrial plants relied on log <em>K</em>_<em>ow</em> more than biological factors. Meanwhile, TIL of Poaceae plants could be a common and key protein that contributed to OPEs accumulation.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154418"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Cutinized and suberized barriers in leaves and roots: Similarities and differences” [J. Plant Physiol. 282 (2023) 153921]","authors":"Paul Grünhofer,&nbsp;Lukas Schreiber","doi":"10.1016/j.jplph.2024.154416","DOIUrl":"10.1016/j.jplph.2024.154416","url":null,"abstract":"","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154416"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wisdom comes after facts – An update on plants priming using phytohormones","authors":"Hubert Matkowski,&nbsp;Agata Daszkowska–Golec","doi":"10.1016/j.jplph.2024.154414","DOIUrl":"10.1016/j.jplph.2024.154414","url":null,"abstract":"<div><div>Currently, agriculture is facing the threat of climate change. Adaptation of plants to unfavorable growth conditions is undoubtedly a great challenge for scientists. A promising solution to this problem is priming, for which chemicals, microorganisms and phytohormones can be used. The use of priming not only affects the adaptation of plants to unfavorable environmental conditions caused by water deficiency, low temperatures, heat and soil pollution, but can also improve the quantity and quality of biomass. In this review, we focus on the role of plant phytohormones in inducing priming in crop plants. We took a closer look at hormones such as abscisic acid, salicylic acid, jasmonic acid and gibberellins. We focused not only on their physiological and morphological effects, but also on what changes at the molecular level are induced by priming with phytohormones. An interesting aspect of priming is the epigenetic changes induced by phytohormones, which influence better adaptation to unfavorable conditions, which is why we addressed this topic in this review.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154414"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trimeric tetrapeptide repeat protein TPR16 positively regulates salt stress in apple","authors":"Qing Wang ,&nbsp;Da-Ru Wang ,&nbsp;Xin Liu ,&nbsp;Guo-Lin Chen,&nbsp;He-Dan Li,&nbsp;Wen-Long Ji,&nbsp;Man-Shu Qu,&nbsp;Rui Yang,&nbsp;Chun-Xiang You","doi":"10.1016/j.jplph.2024.154415","DOIUrl":"10.1016/j.jplph.2024.154415","url":null,"abstract":"<div><div>Plants are vulnerable to various abiotic stresses in the natural growing environment, among which salt stress can seriously affect plant growth, development and yield. Protein families containing trimeric tetrapeptide repeat sequences have a crucial function in plant resilience to non-living factors and participate in multiple aspects of plant growth and development. For this investigation, we acquired the apple <em>MdTPR16</em> gene. The research demonstrated that ectopic expression of <em>MdTPR16</em> in <em>Arabidopsis</em> resulted in increased resistance to salt stress. This was observed by a drop in malondialdehyde (MDA) levels and a reduction in the buildup of reactive oxygen species (ROS) under salt stress conditions. Meanwhile, apple calli, apple seedlings and apple rooting seedlings overexpressing <em>MdTPR16</em> showed reduced sensitivity to salt stress. The results indicate that <em>MdTPR16</em> has a critical positive regulatory function under salt stress, which may lay the foundation for a deeper understanding of the molecular pathways of salt tolerance in apple.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154415"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}