Journal of plant physiology最新文献

{"title":"Metabolite profiling and histochemical localization of alkaloids in Hippeastrum papilio (Ravena) van Scheepen","authors":"Gabriela Haist ,&nbsp;Boriana Sidjimova ,&nbsp;Elina Yankova-Tsvetkova ,&nbsp;Milena Nikolova ,&nbsp;Rumen Denev ,&nbsp;Ivanka Semerdjieva ,&nbsp;Jaume Bastida ,&nbsp;Strahil Berkov","doi":"10.1016/j.jplph.2024.154223","DOIUrl":"10.1016/j.jplph.2024.154223","url":null,"abstract":"<div><p><em>Hippeastrum papilio</em> (Amaryllidaceae) is a promising new source of galanthamine - an alkaloid used for the cognitive treatment of Alzheimer's disease. The biosynthesis and accumulation of alkaloids are tissue - and organ-specific. In the present study, histochemical localization of alkaloids in <em>H. papilio</em>'s plant organs with Dragendorff's reagent, revealed their presence in all studied samples. Alkaloids were observed in vascular bundles, vacuoles, and intracellular spaces, while in other plant tissues and structures depended on the plant organ. The leaf parenchyma and the vascular bundles were indicated as alkaloid-rich structures which together with the high proportion of alkaloids in the phloem sap (49.3% of the Total Ion Current – TIC, measured by GC-MS) indicates the green tissues as a possible site of galanthamine biosynthesis. The bulbs and roots showed higher alkaloid content compared to the leaf parts. The highest alkaloid content was found in the inner bulb part. GC-MS metabolite profiling of <em>H. papilio</em>'s root, bulb, and leaves revealed about 82 metabolites (&gt;0.01% of TIC) in the apolar, polar, and phenolic acid fractions, including organic acids, fatty acids, sterols, sugars, amino acids, free phenolic acids, and conjugated phenolic acids. The most of organic and fatty acids were in the peak part of the root, while the outermost leaf was enriched with sterols. The outer and middle parts of the bulb had the highest amount of saccharides, while the peak part of the middle leaf had most of the amino acids, free and conjugated phenolic acids.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"296 ","pages":"Article 154223"},"PeriodicalIF":4.3,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140125501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of transcription factor genes responsive to MeJA and characterization of a LaMYC2 transcription factor positively regulates lycorine biosynthesis in Lycoris aurea","authors":"Zhe Zhou ,&nbsp;Mingzhu Wu ,&nbsp;Bin Sun ,&nbsp;Jie Li ,&nbsp;Junde Li ,&nbsp;Zhengtai Liu ,&nbsp;Meng Gao ,&nbsp;Lei Xue ,&nbsp;Sheng Xu ,&nbsp;Ren Wang","doi":"10.1016/j.jplph.2024.154218","DOIUrl":"10.1016/j.jplph.2024.154218","url":null,"abstract":"<div><p>Jasmonates (JAs) are among the main phytohormones, regulating plant growth and development, stress responses, and secondary metabolism. As the major regulator of the JA signaling pathway, MYC2 also plays an important role in plant secondary metabolite synthesis and accumulation. In this study, we performed a comparative transcriptome analysis of <em>Lycoris aurea</em> seedlings subjected to methyl jasmonate (MeJA) at different treatment times. A total of 31,193 differentially expressed genes (DEGs) were identified by RNA sequencing. Among them, 732 differentially expressed transcription factors (TFs) comprising 51 TF families were characterized. The most abundant TF family was WRKY proteins (80), followed by AP2/ERF-EFR (67), MYB (59), bHLH (52), and NAC protein (49) families. Subsequently, by calculating the Pearson's correlation coefficient (PCC) between the expression level of TF DEGs and the lycorine contents, 41 potential TF genes (|PCC| &gt;0.8) involved in lycorine accumulation were identified, including 36 positive regulators and 5 negative regulators. Moreover, a MeJA-inducible <em>MYC2</em> gene (namely <em>LaMYC2</em>) was cloned on the basis of transcriptome sequencing. Bioinformatic analyses revealed that LaMYC2 proteins contain the bHLH-MYC_N domain and bHLH-AtAIB_like motif. LaMYC2 protein is localized in the cell nucleus, and can partly rescue the <em>MYC2</em> mutant in <em>Arabidopsis thaliana</em>. LaMYC2 protein could interact with most LaJAZs (especially LaJAZ3 and LaJAZ4) identified previously. Transient overexpression of <em>LaMYC2</em> increased lycorine contents in <em>L. aurea</em> petals, which might be associated with the activation of the transcript levels of <em>tyrosine decarboxylase</em> (<em>TYDC</em>) and <em>phenylalanine ammonia lyase</em> (<em>PAL</em>) genes. By isolating the 887-bp-length promoter fragment upstream of the start codon (ATG) of <em>LaTYDC</em>, we found several different types of E-box motifs (CANNTG) in the promoter of <em>LaTYDC</em>. Further study demonstrated that LaMYC2 was indeed able to bind the E-box (CACATG) present in the <em>LaTYDC</em> promoter, verifying that the pathway genes involved in lycorine biosynthesis could be regulated by LaMYC2, and that LaMYC2 has positive roles in the regulation of lycorine biosynthesis. These findings demonstrate that LaMYC2 is a positive regulator of lycorine biosynthesis and may facilitate further functional research of the <em>LaMYC2</em> gene, especially its potential regulatory roles in Amaryllidaceae alkaloid accumulation in <em>L. aurea</em>.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"296 ","pages":"Article 154218"},"PeriodicalIF":4.3,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140125748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Searching for mechanisms driving root pressure in Zea mays—a transcriptomic approach","authors":"Sarah Tepler Drobnitch ,&nbsp;Joshua Wenz ,&nbsp;Sean M. Gleason ,&nbsp;Louise H. Comas","doi":"10.1016/j.jplph.2024.154209","DOIUrl":"10.1016/j.jplph.2024.154209","url":null,"abstract":"<div><p>While there are many theories and a variety of innovative datasets contributing to our understanding of the mechanism generating root pressure in vascular plants, we are still unable to produce a specific cellular mechanism for any species. To discover these mechanisms, we used RNA-Seq to explore differentially expressed genes in three different tissues between individual <em>Zea mays</em> plants expressing root pressure and those producing none. Working from the perspective that roots cells are utililizing a combination of osmotic exudation and hydraulic pressure mechanisms to generate positively-pressured flow of water into the xylem from the soil, we hypothesized that differential expression analysis would yield candidate genes coding for membrane transporters, ion channels, ATPases, and hormones with clear relevance to root pressure generation. In basal stem and coarse root tissue, we observed these classes of differentially expressed genes and more, including a strong cytoskeletal remodeling response. Fine roots displayed remarkably little differential expression relevant to root pressure, leading us to conclude that they either do not contribute to root pressure generation or are constitutively expressing root pressure mechanisms regardless of soil water content.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"296 ","pages":"Article 154209"},"PeriodicalIF":4.3,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel OsHB5-OsAPL-OsMADS27/OsWRKY102 regulatory module regulates grain size in rice","authors":"Han Zhang ,&nbsp;Meng Liu ,&nbsp;Kangqun Yin ,&nbsp;Huanhuan Liu ,&nbsp;Jianquan Liu ,&nbsp;Zhen Yan","doi":"10.1016/j.jplph.2024.154210","DOIUrl":"10.1016/j.jplph.2024.154210","url":null,"abstract":"<div><p>Grain size, a crucial trait that determines rice yield and quality, is typically regulated by multiple genes. Although numerous genes controlling grain size have been identified, the precise and dynamic regulatory network governing grain size is still not fully understood. In this study, we unveiled a novel regulatory module composed of OsHB5, OsAPL and <em>OsMADS27</em>/<em>OsWRKY102</em>, which plays a crucial role in modulating grain size in rice. As a positive regulator of grain size, OsAPL has been found to interact with OsHB5 both in vitro and in vivo. Through chromatin immunoprecipitation-sequencing, we successfully mapped two potential targets of OsAPL, namely <em>OsMADS27</em>, a positive regulator in grain size and <em>OsWRKY102</em>, a negative regulator in lignification that is also associated with grain size control. Further evidence from EMSA and chromatin immunoprecipitation-quantitative PCR experiments has shown that OsAPL acts as an upstream transcription factor that directly binds to the promoters of <em>OsMADS27</em> and <em>OsWRKY102</em>. Moreover, EMSA and dual-luciferase reporter assays have indicated that the interaction between OsAPL and OsHB5 enhances the repressive effect of OsAPL on <em>OsMADS27</em> and <em>OsWRKY102</em>. Collectively, our findings discovered a novel regulatory module, OsHB5-OsAPL-OsMADS27/OsWRKY102, which plays a significant role in controlling grain size in rice. These discoveries provide potential targets for breeding high-yield and high-quality rice varieties.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"295 ","pages":"Article 154210"},"PeriodicalIF":4.3,"publicationDate":"2024-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0176161724000415/pdfft?md5=023544e9fad82a4fbab5686ed9ed9e54&pid=1-s2.0-S0176161724000415-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the TOR pathway on plant growth via cell wall remodeling","authors":"Maria Juliana Calderan-Rodrigues ,&nbsp;Camila Caldana","doi":"10.1016/j.jplph.2024.154202","DOIUrl":"10.1016/j.jplph.2024.154202","url":null,"abstract":"<div><p>Plant growth is intimately linked to the availability of carbon and energy status. The Target of rapamycin (TOR) pathway is a highly relevant metabolic sensor and integrator of plant-assimilated C into development and growth. The cell wall accounts for around a third of the cell biomass, and the investment of C into this structure should be finely tuned for optimal growth. The plant C status plays a significant role in controlling the rate of cell wall synthesis. TOR signaling regulates cell growth and expansion, which are fundamental processes for plant development. The availability of nutrients and energy, sensed and integrated by TOR, influences cell division and elongation, ultimately impacting the synthesis and deposition of cell wall components. The plant cell wall is crucial in environmental adaptation and stress responses. TOR senses and internalizes various environmental cues, such as nutrient availability and stresses. These environmental factors influence TOR activity, which modulates cell wall remodeling to cope with changing conditions. Plant hormones, including auxins, gibberellins, and brassinosteroids, also regulate TOR signaling and cell wall-related processes. The connection between nutrients and cell wall pathways modulated by TOR are discussed.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"294 ","pages":"Article 154202"},"PeriodicalIF":4.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139945427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification candidate genes for salt resistance through quantitative trait loci-sequencing in Brassica napus L.","authors":"Yan Zhang ,&nbsp;Zhiting Guo ,&nbsp;Xiaoqin Chen ,&nbsp;Xinru Li ,&nbsp;Yiji Shi ,&nbsp;Liang Xu ,&nbsp;Chengyu Yu ,&nbsp;Bing Jing ,&nbsp;Weiwei Li ,&nbsp;Aixia Xu ,&nbsp;Xue Shi ,&nbsp;Keqi Li ,&nbsp;Zhen Huang","doi":"10.1016/j.jplph.2024.154187","DOIUrl":"10.1016/j.jplph.2024.154187","url":null,"abstract":"<div><p>Rapeseed (<em>Brassica napus</em> L.) is one of the most important oil crops worldwide. However, its yield is greatly limited by salt stress, one of the primary abiotic stresses. Identification of salt-tolerance genes and breeding salt-tolerant varieties is an effective approach to address this issue. Unfortunately, little is known about the salt-tolerance quantitative trait locus (QTL) and the identification of salt tolerance genes in rapeseed. In this study, high-throughput quantitative trait locus sequencing (QTL-seq) was applied to identifying salt-tolerant major QTLs based on two DNA pools from an F_2:3 population of a cross between rapeseed line 2205 (salt tolerant) and 1423 (salt sensitive). A total of twelve major QTLs related to the salt tolerance rating (STR) were detected on chromosomes A03, A08, C02, C03, C04, C06, C07 and C09. To further enhance our understanding, we integrated QTL-seq data with transcriptome analysis of the two parental rapeseed plants subjected to salt stress, through which ten candidate genes for salt tolerance were identified within the major QTLs by gene differential expression, variation and annotated functions analysis. The marker SNP820 linked to salt tolerance was successfully validated and would be useful as a diagnostic marker in marker-assisted breeding. These findings provide valuable insights for future breeding programs aimed at developing rapeseed cultivars resistant to salt stresses.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"294 ","pages":"Article 154187"},"PeriodicalIF":4.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139688826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic changes during wheat microspore embryogenesis induction using the highly responsive cultivar Svilena","authors":"Teresa Perez-Piñar ,&nbsp;Anja Hartmann ,&nbsp;Sandra Bössow ,&nbsp;Heike Gnad ,&nbsp;Hans-Peter Mock","doi":"10.1016/j.jplph.2024.154193","DOIUrl":"10.1016/j.jplph.2024.154193","url":null,"abstract":"<div><p>Androgenetically-derived haploids can be obtained by inducing embryogenesis in microspores. Thus, full homozygosity is achieved in a single generation, oppositely to conventional plant breeding programs. Here, the metabolite profile of embryogenic microspores of <em>Triticum aestivum</em> was acquired and integrated with transcriptomic existing data from the same samples in an effort to identify the key metabolic processes occurring during the early stages of microspore embryogenesis. Primary metabolites and transcription profiles were identified at three time points: prior to and immediately following a low temperature pre-treatment given to uninuclear microspores, and after the first nuclear division. This is the first time an integrative -omics analysis is reported in microspore embryogenesis in <em>T. aestivum</em>. The key findings were that the energy produced during the pre-treatment was obtained from the tricarboxylic acid (TCA) cycle and from starch degradation, while starch storage resumed after the first nuclear division. Intermediates of the TCA cycle were highly demanded from a very active amino acid metabolism. The transcription profiles of genes encoding enzymes involved in amino acid synthesis differed from the metabolite profiles. The abundance of glutamine synthetase was correlated with that of glutamine. Cytosolic glutamine synthetase isoform 1 was found predominantly after the nuclear division. Overall, energy production was shown to represent a major component of the de-differentiation process induced by the pre-treatment, supporting a highly active amino acid metabolism.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"294 ","pages":"Article 154193"},"PeriodicalIF":4.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0176161724000245/pdfft?md5=8d3b602f98b509d1e0922177d7d6deac&pid=1-s2.0-S0176161724000245-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139824004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of MsNIP2 improves salinity tolerance in Medicago sativa","authors":"Weiye Kong ,&nbsp;Haijun Huang ,&nbsp;Wenxuan Du ,&nbsp;Zhihu Jiang ,&nbsp;Yijing Luo ,&nbsp;Dengxia Yi ,&nbsp;Guofeng Yang ,&nbsp;Yongzhen Pang","doi":"10.1016/j.jplph.2024.154207","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154207","url":null,"abstract":"<div><p>Alfalfa (<em>Medicago sativa</em>) is one of the most widely cultivated forage crops in the world. However, alfalfa yield and quality are adversely affected by salinity stress. Nodulin 26-like intrinsic proteins (NIPs) play essential roles in water and small molecules transport and response to salt stress. Here, we isolated a salt stress responsive <em>MsNIP2</em> gene and demonstrated its functions by overexpression in alfalfa. The open reading frame of <em>MsNIP2</em> is 816 bp in length, and it encodes 272 amino acids. It has six transmembrane domains and two NPA motifs. MsNIP2 showed high identity to other known NIP proteins, and its tertiary model was similar to the crystal structure of OsNIP2-1 (7cjs) tetramer. Subcellular localization analysis showed that MsNIP2 protein fused with green fluorescent protein (GFP) was localized to the plasma membrane. Transgenic alfalfa lines overexpressing <em>MsNIP2</em> showed significantly higher height and branch number compared with the non-transgenic control. The POD and CAT activity of the transgenic alfalfa lines was significantly increased and their MDA content was notably reduced compared with the control group under the treatment of NaCl. The transgenic lines showed higher capability in scavenging oxygen radicals with lighter NBT staining than the control under salt stress. The transgenic lines showed relative lower water loss rate and electrolyte leakage, but relatively higher Na⁺ content than the control line under salt stress. The relative expression levels of abiotic-stress-related genes (<em>MsHSP23</em>, <em>MsCOR47</em>, <em>MsATPase</em>, and <em>MsRD2</em>) in three transgenic lines were compared with the control, among them, only the expression of <em>MsCOR47</em> was up-regulated. Consequently, this study offers a novel perspective for exploring the function of <em>MsNIP2</em> in improving salt tolerance of alfalfa.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"295 ","pages":"Article 154207"},"PeriodicalIF":4.3,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140000368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolism and development","authors":"Alisdair R. Fernie,&nbsp;Uwe Sonnewald,&nbsp;Arun Sampathkumar","doi":"10.1016/j.jplph.2024.154208","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154208","url":null,"abstract":"","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"295 ","pages":"Article 154208"},"PeriodicalIF":4.3,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140096062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic control of seed germination in legumes","authors":"Júlia de Paiva Gonçalves ,&nbsp;Karla Gasparini ,&nbsp;Edgard Augusto de Toledo Picoli ,&nbsp;Maximiller Dal-Bianco Lamas Costa ,&nbsp;Wagner Luiz Araujo ,&nbsp;Agustin Zsögön ,&nbsp;Dimas Mendes Ribeiro","doi":"10.1016/j.jplph.2024.154206","DOIUrl":"10.1016/j.jplph.2024.154206","url":null,"abstract":"<div><p>Seed development, dormancy, and germination are connected with changes in metabolite levels. Not surprisingly, a complex regulatory network modulates biosynthesis and accumulation of storage products. Seed development has been studied profusely in <em>Arabidopsis thaliana</em> and has provided valuable insights into the genetic control of embryo development. However, not every inference applies to crop legumes, as these have been domesticated and selected for high seed yield and specific metabolic profiles and fluxes. Given its enormous economic relevance, considerable work has contributed to shed light on the mechanisms that control legume seed growth and germination. Here, we summarize recent progress in the understanding of regulatory networks that coordinate seed metabolism and development in legumes.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"295 ","pages":"Article 154206"},"PeriodicalIF":4.3,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139945426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}