Journal of plant physiology最新文献

WEE1 homolog positively regulates salt stress tolerance in chrysanthemum WEE1同源物正调控菊花耐盐性

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-17 DOI: 10.1016/j.jplph.2025.154550

Yahui Wang , Fengze Sun , Zhilong Bao, Fangfang Ma

{"title":"WEE1 homolog positively regulates salt stress tolerance in chrysanthemum","authors":"Yahui Wang , Fengze Sun , Zhilong Bao, Fangfang Ma","doi":"10.1016/j.jplph.2025.154550","DOIUrl":"10.1016/j.jplph.2025.154550","url":null,"abstract":"<div><div>Salinity is a major abiotic stress that limits chrysanthemum yields worldwide. Salinity represses <em>Chrysanthemum lavandulifolium</em> plant growth and consequently reduces chrysanthemum commercial production. Salinity triggers DNA damage in root cells, leading to cell death and subsequent growth repression. <em>WEE1</em> plays an important role in regulating DNA repair, although its function in salt tolerance has not been studied in <em>C. lavandulifolium</em>. In this study, we identify <em>WEE1</em> homologous genes in <em>Chrysanthemum</em> species, and their expressions are induced in roots after salt stress treatment. We further investigate the function of <em>C. lavandulifolium</em> homolog <em>ClWEE1</em> in salt stress responses and find that <em>ClWEE1</em> plays a crucial role in cell cycle regulation and DNA damage repair under salt stress. Overexpressing <em>ClWEE1</em> in <em>C. lavandulifolium</em> or <em>Arabidopsis</em> significantly enhances their salt stress tolerance. Both flow cytometric analysis and comet assay reveal less DNA damage in <em>ClWEE1</em>-overexpression plants than in wild type. RT-qPCR analysis indicates that the stress-responsive genes <em>ClNHX</em>, <em>ClHKT</em>, <em>ClCBL</em>, and <em>ClDREB2A</em> may have higher expression in <em>ClWEE1</em>-overexpression plants than in wild type. Taken together, our study illustrates the positive role of <em>Chrysanthemum WEE1</em> in enhancing salt tolerance, providing insights for breeding salt-tolerant chrysanthemum varieties.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154550"},"PeriodicalIF":4.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of the journey of field crop phenotyping: From trait stamp collections and fancy robots to phenomics-informed crop performance predictions 回顾田间作物表型的历程：从性状邮票收集和花哨的机器人到基于表型的作物性能预测

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-13 DOI: 10.1016/j.jplph.2025.154542

Lukas Roth, Afef Marzougui, Achim Walter

{"title":"A review of the journey of field crop phenotyping: From trait stamp collections and fancy robots to phenomics-informed crop performance predictions","authors":"Lukas Roth, Afef Marzougui, Achim Walter","doi":"10.1016/j.jplph.2025.154542","DOIUrl":"10.1016/j.jplph.2025.154542","url":null,"abstract":"<div><div>Crop phenotyping encompasses methodologies for measuring plant growth, architecture, and composition with high precision across scales, from organs to canopies. Field-based phenotyping is pivotal in bridging genomic data with crop performance, offering a promising pathway for predictive modeling in diverse environments. This review traces the evolution of phenotyping from high-throughput sensor data for trait extraction to advanced modeling approaches that integrate multi-temporal data, latent space representations, and learned crop models. This evolution is exemplified mostly by morphology- and growth-related examples from the core expertise of the authors. High-throughput trait extraction, facilitated by advanced imaging and sensor technologies, has enabled rapid and accurate characterization of complex traits essential for crop improvement. Carrier platforms, such as drones, rovers, and gantries, have played a critical role in capturing high-resolution data across large fields, enhancing the spatial and temporal resolution of phenotypic data. Publicly available datasets have further accelerated research by providing standardized, high-quality data for benchmarking and model development beyond the realm of crop growth as for example in crop photosynthesis. These advancements are transforming phenotyping into a predictive science capable of informing breeding and management decisions. As phenotyping methodologies continue to evolve, the integration of machine learning and data-driven approaches offers new opportunities for enhancing prediction accuracy and understanding genotype-environment interactions. While challenges such as data heterogeneity, scalability, and cost remain, we highlight key gaps and propose solutions, underscoring phenotyping's critical role in future agricultural innovation.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154542"},"PeriodicalIF":4.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

●Dynamic changes of rice sheath non-structural carbohydrates and source-sink balance under elevated atmospheric CO2 concentration and temperature stresses ●大气CO2浓度升高和温度胁迫下水稻鞘非结构性碳水化合物及源库平衡的动态变化

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-12 DOI: 10.1016/j.jplph.2025.154547

Hao He , Jiabin Shi , Mingjie Chen , Jing Zhao , Kexi Qu , Man Li , Lin Zhao , Yuanyuan Wang , Zhenghua Hu , Qi Li

{"title":"●Dynamic changes of rice sheath non-structural carbohydrates and source-sink balance under elevated atmospheric CO2 concentration and temperature stresses","authors":"Hao He , Jiabin Shi , Mingjie Chen , Jing Zhao , Kexi Qu , Man Li , Lin Zhao , Yuanyuan Wang , Zhenghua Hu , Qi Li","doi":"10.1016/j.jplph.2025.154547","DOIUrl":"10.1016/j.jplph.2025.154547","url":null,"abstract":"<div><div>Rising CO<sub>2</sub> levels and temperatures significantly affect rice yield and quality by altering key physiological processes. As vital carbon reserves, non-structural carbohydrates (NSC) maintain the source-sink balance, directly influencing grain filling and food security. This study simulated high CO<sub>2</sub> and temperature conditions using open-top chambers with four treatment groups: control (ACT, ambient CO<sub>2</sub> at 415 ppm and temperature), elevated CO<sub>2</sub> (EC, 600 ppm CO<sub>2</sub>), elevated temperature (ET, ambient +2°C), and combined CO<sub>2</sub> and temperature elevation (ECT, 600 ppm CO<sub>2</sub> +2°C). NSC concentrations, along with key physiological indexes such as leaf nitrogen and antioxidant enzyme activity, and gene expression, was measured to assess climate impacts on <em>japonica</em> rice variety “Nanjing 9108” physiology and source-sink balance. This study found that EC enhanced NSC concentrations, increasing soluble sugars and starch by 6.33% and 9.86% at heading, raising stem sheath NSC by 9.30%. Conversely, ET reduced sugars and starch by 16.67% and 6.24%, leading to a 7.75% NSC decrease. Under ECT, NSC levels dropped by 1.07%. Nitrogen concentrations in leaves, stem sheaths, and panicles declined under both EC and ET, EC reduced leaf nitrogen by 16.26%, while ET lowered nitrogen in stem sheaths and panicles by 17.29% and 16.53%. EC upregulated <em>OsSUT1</em> and <em>OsSUT2</em> gene expression by 69.55% and 131.85%, boosting carbon transport, whereas ET suppressed those genes, reducing grain carbon supply. Overall, elevated CO<sub>2</sub> improves NSC accumulation and transport, enhancing yield potential, while elevated temperature hinders these processes. Managing NSC and nitrogen dynamics is crucial to ensure stable rice yields under climate change.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154547"},"PeriodicalIF":4.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Plant Alkaloid Biosynthesis under Drought Stress: Regulatory Mechanisms and Biotechnological Strategies 干旱胁迫下植物生物碱合成优化：调控机制与生物技术策略

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-10 DOI: 10.1016/j.jplph.2025.154545

Ziyi Guo , Sujing He , Xinying Zhong , Ning Yang , Delin Xu

{"title":"Optimizing Plant Alkaloid Biosynthesis under Drought Stress: Regulatory Mechanisms and Biotechnological Strategies","authors":"Ziyi Guo , Sujing He , Xinying Zhong , Ning Yang , Delin Xu","doi":"10.1016/j.jplph.2025.154545","DOIUrl":"10.1016/j.jplph.2025.154545","url":null,"abstract":"<div><div>Global climate change exacerbates drought stress, severely affecting plant growth, agricultural productivity, and the biosynthesis of secondary metabolites. Alkaloids, nitrogenous compounds with diverse biological activities, hold substantial medicinal value across various plant species. This review investigates the regulatory mechanisms through which drought stress influences alkaloid synthesis, focusing on key pathways such as abscisic acid (ABA) signaling and reactive oxygen species (ROS) responses that modulate gene expression and metabolic processes. Furthermore, we explore advanced biotechnological strategies—including genetic engineering, synthetic biology, and artificial intelligence (AI)—designed to optimizing alkaloid production under drought stress conditions. In agriculture, these strategies support the development of drought-resistant crops with enhanced alkaloid profiles, while in the pharmaceutical industry, sustainable production methods for valuable alkaloids are highlighted. The review also addresses critical challenges, such as balancing plant growth with metabolite production and ensuring field-level applicability of laboratory-developed strategies. By emphasizing interdisciplinary collaboration, this research provides comprehensive insights and practical guidance for enhancing crop resilience and maximizing alkaloid yields, thereby advancing sustainability in the medicinal plant industry.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154545"},"PeriodicalIF":4.0,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing microspore embryogenesis initiation by reducing ROS, autophagy, and cell death with novel small molecules in rapeseed and barley 通过减少活性氧、自噬和细胞死亡的新型小分子在油菜籽和大麦中促进小孢子胚胎发生

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-10 DOI: 10.1016/j.jplph.2025.154546

Cristina Rueda-Varela , Elena Carneros , Elena Caro , Yolanda Pérez-Pérez , Alfonso García-Rubia , Ana Martínez , Carmen Gil , Pilar S. Testillano

{"title":"Enhancing microspore embryogenesis initiation by reducing ROS, autophagy, and cell death with novel small molecules in rapeseed and barley","authors":"Cristina Rueda-Varela , Elena Carneros , Elena Caro , Yolanda Pérez-Pérez , Alfonso García-Rubia , Ana Martínez , Carmen Gil , Pilar S. Testillano","doi":"10.1016/j.jplph.2025.154546","DOIUrl":"10.1016/j.jplph.2025.154546","url":null,"abstract":"<div><div>When submitted to stress conditions, microspores cultured <em>in vitro</em> can be reprogrammed towards an embryogenic pathway, the so-called microspore embryogenesis, which constitutes a biotechnological tool to rapidly produce double-haploid plants for breeding programs. Nevertheless, not all cells succeed in switching their development and, as a consequence of the stress treatment applied, many of them undergo cell death, which causes a significant reduction of the final yield of the process. In this study, we have analyzed the potential of several novel small molecule antioxidants, never used before in plants, to improve cell viability during microspore embryogenesis induction. The new molecules have been tested in two crop species, <em>Brassica napus</em> and <em>Hordeum vulgare</em>, in which cell reprograming was induced by heat (32 °C) and cold (4 °C) treatments. Using transcriptomic and physiological approaches, we have analyzed changes in oxidative stress and autophagy, and their involvement in cell death during microspore embryogenesis induction. The results provide new evidence of increased ROS production and upregulated oxidative stress and autophagy-related genes during embryogenesis induction, all of which contribute to higher cell death. We identified novel small molecule antioxidants that mitigated these effects, enhancing cell viability and promoting microspore embryogenesis initiation. The findings in two phylogenetically distant crop species suggest a conserved cellular response and highlight the potential of these compounds to improve <em>in vitro</em> protocols in other species where early-stage cell death poses a significant challenge during embryogenesis induction.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154546"},"PeriodicalIF":4.0,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and functional study of AdOPT1, a gene for synthesizing isoimperatorin from Angelica dahurica var. formosana 台湾白芷异欧前胡素合成基因AdOPT1的克隆及功能研究

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-07 DOI: 10.1016/j.jplph.2025.154543

Changguo Nong, Yijie Jiang, Yanan Liu, Jiaheng Zhang, Dongbei Xu, Kai Hou, Wei Wu

{"title":"Cloning and functional study of AdOPT1, a gene for synthesizing isoimperatorin from Angelica dahurica var. formosana","authors":"Changguo Nong, Yijie Jiang, Yanan Liu, Jiaheng Zhang, Dongbei Xu, Kai Hou, Wei Wu","doi":"10.1016/j.jplph.2025.154543","DOIUrl":"10.1016/j.jplph.2025.154543","url":null,"abstract":"<div><div><em>Angelica dahurica</em> var. <em>formosana</em> (<em>ADF</em>) is a perennial medicinal plant in the Apiaceae family. Its dried roots are rich in furanocoumarins, which exhibit significant pharmacological activities and development potential. The main coumarins in <em>ADF</em> are imperatorin and isoimperatorin. The gene responsible for imperatorin biosynthesis has been reported, but the gene catalyzing isoimperatorin synthesis remains unknown. This study identified a candidate gene, <em>AdOPT1</em> (1230 bp), from the transcriptome data of <em>ADF</em> through homology-based comparison with <em>AkPT1</em> in <em>Angelica keiskei</em>, showing 93 % similarity. <em>In vitro</em> functional assays revealed that AdOPT1 catalyzes the oxygen-prenylation reaction between DMAPP and bergaptol to produce isoimperatorin. The kinetic parameters of AdOPT1 for bergaptol and DMAPP were found to be Km bergaptol = 112.9 ± 17.83 μM, Km DMAPP = 1.97 ± 0.08 μM, respectively. Molecular docking and alanine scanning analysis identified critical residues 45Lys, 124His, and 395Trp for AdOPT1 catalytic activity. Under various abiotic stresses such as flooding, salt, drought, and shading treatment, the expression level of <em>AdOPT1</em> gene in <em>ADF</em> significantly increased, suggesting its involvement in <em>ADF</em> stress resistance. Additionally, transient overexpression of <em>AdOPT1</em> significantly increased isoimperatorin content, with 3.26-fold higher than the control-group and 3.53-fold higher than the wild-type. It further confirmed that AdOPT1 is responsible for catalyzing the biosynthesis of isoimperatorin in <em>ADF</em>. This provides a molecular theoretical basis for making the biosynthesis pathway of furanocoumarin more comprehensive and helps to breed new high-quality <em>ADF</em> varieties in the future.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154543"},"PeriodicalIF":4.0,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High throughput phenotyping using automated imaging system reveals the relationship between seed yield and agronomic traits in Korean rice cultivars 利用自动成像系统进行高通量表型分析，揭示了韩国水稻品种籽粒产量与农艺性状之间的关系

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-06 DOI: 10.1016/j.jplph.2025.154544

Joon Ki Hong , Jeongho Baek , Jae Young Kim , Song Lim Kim , Jae Il Lyu , Sang-Ho Kang , Jiseon Song , Nyunhee Kim , Eunsook An , Hyun-Sook Lee , Kyung-Hwan Kim , Yong Suk Chung , Sheikh Mansoor

{"title":"High throughput phenotyping using automated imaging system reveals the relationship between seed yield and agronomic traits in Korean rice cultivars","authors":"Joon Ki Hong , Jeongho Baek , Jae Young Kim , Song Lim Kim , Jae Il Lyu , Sang-Ho Kang , Jiseon Song , Nyunhee Kim , Eunsook An , Hyun-Sook Lee , Kyung-Hwan Kim , Yong Suk Chung , Sheikh Mansoor","doi":"10.1016/j.jplph.2025.154544","DOIUrl":"10.1016/j.jplph.2025.154544","url":null,"abstract":"<div><div>This study utilized plant phenomics image analysis technology to explore the agronomic characteristics of rice cultivars, aiming to enhance growth stability, yield potential, and digital data for rice breeding. RGB images were captured at three lateral angles during the growth period of the plants using ScanLyzer, LemnaTec. A total of 42 agronomic traits were analyzed across 102 rice cultivars, categorized into three maturing groups. In addition, to evaluate the measurement accuracy, 9 phenotypic traits, the panicle length (Pl), panicle count (Pc), and number of seeds were also measured destructively after harvest. Parameter estimated revealed that the Pl trait exerted the strongest positive effect on seed production across all groups analyzed, with coefficients (β) of 0.459 for the entire population, 0.456 in the early-maturing group, 0.537 in the medium-maturing group, and 0.574 in the medium-late maturing group <em>(p < 0.05).</em> Other traits, such as maximum area (Am), and maximum height (Hm), also positively influenced seed production but to a lesser extent. Notably, duration of maximum value of rice plant width had a significant negative effect in the early-maturing group <em>(β = -0.369, p < 0.05).</em> Correlation analyses revealed strong positive relationships between seed production and various traits across maturity classes, notably with days to maximum height, Pl, Pc, and seed count. Additionally, panicle length and count emerged as pivotal factors influencing seed numbers. These findings underscore the varying impacts of agronomic traits on seed yield depending on cultivars and maturity groups, offering valuable insights for the selection of rice cultivars aimed at optimizing seed production.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154544"},"PeriodicalIF":4.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of rice varieties with low accumulation of heavy metals based on leaf morphology 基于叶片形态的重金属低积累水稻品种筛选

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-04 DOI: 10.1016/j.jplph.2025.154540

Xingyuan Shao , Pengyue Yu , Min Zuo , Zhenglong Tong , Zhi Huang , Zhiyan Xie , Ruimin Chang , Jianwei Peng , Yaocheng Deng , Ying Huang

{"title":"Screening of rice varieties with low accumulation of heavy metals based on leaf morphology","authors":"Xingyuan Shao , Pengyue Yu , Min Zuo , Zhenglong Tong , Zhi Huang , Zhiyan Xie , Ruimin Chang , Jianwei Peng , Yaocheng Deng , Ying Huang","doi":"10.1016/j.jplph.2025.154540","DOIUrl":"10.1016/j.jplph.2025.154540","url":null,"abstract":"<div><div>This study aimed to investigate the influence of the morphological traits of rice leaves (stomata, leaf area, and trichome density) on the accumulation of heavy metals in rice grains. It also screens for varieties with low heavy metal accumulation and high adaptability (less foliar uptake). Field trials on 37 rice varieties and pot trials regulated by abscisic acid (ABA) were conducted to explore the effects of various leaf surface morphologies and stomata on cadmium (Cd) accumulation in brown rice. Analyzing leaf morphology in different rice varieties revealed that five with high Cd content had an 8.92 %–38.49 % increase in stomatal pore area compared to the average. Meanwhile, these five rice varieties with high Cd content exhibited xylem Cd transit rates above average, ranging from 9.22 % to 182.58 %. Transporter factors throughout the rice plant indicated that the processes from stem-leaf to rachis and husk to brown rice are crucial for Cd accumulation in rice. The results of the correlation and structural equation modelling indicated a significant correlation between rice leaf stomata and the transit rate of Cd in rice xylem. ABA spraying experiments showed that stomata regulate rice transpiration and affect Cd transport and accumulation in rice. Results showed that ABA spraying significantly reduced Cd content in brown rice by 2.4 %–24 % and transpiration rate (33.17 %–54.45 %). Ultimately, the variety ZhuLiangYou 35, characterized by its smaller stomatal pore area and reduced heavy metal concentration in brown rice, is recommended as an appropriate choice.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154540"},"PeriodicalIF":4.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial respiration dynamics in grape exocarp: electron partitioning to AOX pathway during pre-veraison development 葡萄外果皮线粒体呼吸动力学：转化前发育过程中AOX通路的电子分配

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-06-04 DOI: 10.1016/j.jplph.2025.154541

Néstor Fernández Del-Saz , Esther Hernández-Montes

{"title":"Mitochondrial respiration dynamics in grape exocarp: electron partitioning to AOX pathway during pre-veraison development","authors":"Néstor Fernández Del-Saz , Esther Hernández-Montes","doi":"10.1016/j.jplph.2025.154541","DOIUrl":"10.1016/j.jplph.2025.154541","url":null,"abstract":"<div><div>Fruit respiration varies throughout development, reflecting dynamic changes in energy demand and metabolic activity. Grapes grow more rapidly during the preveraison stage, as cell division and expansion drive berry enlargement before the onset of ripening. The plant mitochondrial electron transport chain contains two terminal oxidases; alternative oxidase (AOX) and cytochrome <em>c</em> oxidase (COX), the activities of which are central to predicting patterns of growth in plant organs. However, no previous studies have tested their activities in fruits when growth respiration predominates. Studies about the effect of the genetic variability in grape respiration during phase I of development are scarce. We characterized respiratory parameters <em>in vivo</em> (AOX and COX), and modelled ATP production, at five consecutive weeks during preveraison stage in grape exocarps of two varieties of <em>Vitis vinifera</em> (Merlot and Callet) under well-watered conditions. We also evaluated physical and chemical parameters in fruits (weight, diameter, malic acid and total soluble solids). We identified relationships between respiration via COX activity and carbon accumulation that indicated that ATP production is crucial for the growth of grape berries before the lag phase. After this, AOX activity was increased in parallel to malic acid content in fruit, to lower the reduction level of ETC components when ATP is less required for carbon accumulation. Further studies in more fruit layers, and during ripening, are needed to identify the metabolic role of AOX in grapes.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154541"},"PeriodicalIF":4.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen sulfide: A tiny molecule with a big role in stomatal regulation 硫化氢：一种在气孔调节中起重要作用的微小分子

IF 4 3区生物学

Journal of plant physiology Pub Date : 2025-05-31 DOI: 10.1016/j.jplph.2025.154539

Zhuping Jin , Carlos García‐Mata , Yanxi Pei

引用次数: 0