Journal of plant physiology最新文献

{"title":"Overexpression of AtBES1D in tomato enhances BR response and accelerates fruit ripening","authors":"Sen He ,&nbsp;Xiubo Xia ,&nbsp;Junhui Yang ,&nbsp;Jinyang Xin ,&nbsp;Siqi Chen ,&nbsp;Chengguo Jia","doi":"10.1016/j.jplph.2025.154563","DOIUrl":"10.1016/j.jplph.2025.154563","url":null,"abstract":"<div><div>Brassinosteroids (BRs) are essential plant hormones that regulate growth and development, with BRI1-EMS SUPPRESSOR 1 (BES1) and BRASSINAZOLE-RESISTANT 1 (BZR1) serving as central transcription factors in BR signaling. However, the role of BES1 in regulating tomato fruit ripening remains poorly understood. Here, we generated three independent transgenic tomato lines overexpressing <em>Arabidopsis thaliana BES1D</em> (<em>AtBES1D</em>). Overexpression of <em>AtBES1D</em> enhanced BR responses, as demonstrated by enhanced responsiveness to BRs and reduced sensitivity to the BR biosynthesis inhibitor brassinazole (BRZ). <em>AtBES1D-</em>transgenic tomato plants exhibited pleiotropic phenotypic alterations, including stunted growth, curled leaves, suppressed root elongation, delayed flowering, accelerated fruit ripening, and diminished fruit size, weight, and seed number. In addition, <em>AtBES1D</em> transgenic fruits exhibited upregulated expression of ethylene-related genes (<em>ACS4</em>, <em>NR</em>, <em>ERF1</em>, <em>ERF4</em>, <em>E4</em>, and <em>E8</em>) and ripening regulators (<em>RIN</em>, <em>TAGL1</em>, <em>FUL1</em>, <em>FUL2</em>, and <em>PG</em>). Chromatin immunoprecipitation sequencing (ChIP-seq) identified 1757 AtBES1D target genes, predominantly enriched in plant hormone signaling, transcriptional regulation, and metabolic pathways. Collectively, these findings establish AtBES1D as a multifunctional regulator modulating vegetative development, reproductive transition, and fruit ripening in tomato. AtBES1D likely promotes fruit ripening and improves fruit quality by modulating BR signaling, ethylene pathways, transcription factors, and metabolic processes.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154563"},"PeriodicalIF":4.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting biochemical compositions and microbial interactions of English oak and black poplar root mucilage","authors":"Meisam Nazari ,&nbsp;Mathilde Knott ,&nbsp;Yakov Kuzyakov ,&nbsp;Marta Sena-Velez ,&nbsp;Sylvain Bourgerie ,&nbsp;Sabine Carpin ,&nbsp;Frédéric Lamblin","doi":"10.1016/j.jplph.2025.154561","DOIUrl":"10.1016/j.jplph.2025.154561","url":null,"abstract":"<div><div>Root mucilage plays a crucial role in plant-soil interactions, yet its composition and functions for trees remain largely unexplored. We investigated the root mucilage of two tree species with contrasting growth strategies: the slow-growing English oak (<em>Quercus robur</em> L.) and the fast-growing black poplar (<em>Populus nigra</em> L.). Our analyses focused on the polysaccharide composition of mucilage and its microbial interactions. English oak mucilage polysaccharides consisted of 54% galactose, 16% mannose, 11% arabinose, 7% xylose, and 12% glucuronic acid, with no detectable glucose or galacturonic acid. In contrast, black poplar mucilage polysaccharides contained 26% galactose, 14% mannose, 14% glucose, 22% arabinose, 7% xylose, 14% glucuronic acid, and 3% galacturonic acid. Both mucilage types were hexose-rich, resembling the hexose-to-pentose ratio common in microbial sources in soil. Black poplar mucilage had a higher uronic acid-to-neutral monosaccharide ratio and greater K⁺ and Na⁺ concentrations than English oak mucilage. Functionally, black poplar mucilage increased the growth of <em>Pseudomonas fluorescens</em> SBW25, suggesting the provision of readily available carbon sources. Conversely, English oak mucilage suppressed bacterial growth, plausibly due to antimicrobial compounds that may slow microbial decomposition and promote carbon sequestration.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154561"},"PeriodicalIF":4.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genetic dissection of yield-related traits utilizing quantitative trait loci sequencing approach in mungbean","authors":"Fan Yang ,&nbsp;Jiaqi Qiao ,&nbsp;Bin Li ,&nbsp;Xiao Zhang ,&nbsp;Dongye Liu ,&nbsp;Bojing Wang ,&nbsp;Aili Wei ,&nbsp;Dongao Huo","doi":"10.1016/j.jplph.2025.154552","DOIUrl":"10.1016/j.jplph.2025.154552","url":null,"abstract":"<div><div>Mungbean [<em>Vigna radiata</em> (L.) R. Wilczek] has gained significant popularity in the food industry, due to its distinctive functional properties and exceptional nutritional value. Increasing yield is a central objective in mungbean breeding programs; however, systematic studies identifying quantitative trait loci (QTLs) associated with key yield-related traits remain limited. In this study, the recombinant inbred line (RIL) population (AH20 × SX36) was generated, and phenotypic assessments were conducted in three distinct environments. Three methods genome-wide composite interval mapping (GCIM), multiple QTL mapping (MQM) and inclusive composite interval mapping (ICIM) were employed to detect QTLs linked to HSW (hundred-seed weight), SPP (number of seeds per pod), PL (pod length), PW (pod width), and YP (yield per plant). Consequently, 33, 19, 26, 22, and 20 QTLs were identified for HSW, SPP, PL, PW, and YP, respectively. Notably, 10 QTLs were consistently detected across all environments and by all three mapping methods, indicating their robustness and potential for breeding applications. Candidate genes associated with these stable QTLs were also predicted, offering insights into the genetic regulation of yield traits. These findings provide a valuable genetic framework for functional validation and the cultivation of high-yielding mungbean germplasm.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154552"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The MeCNL3-MeARF6 module plays an important role in cassava bacterial blight resistance","authors":"Yingying Li ,&nbsp;Rongjiao Zheng ,&nbsp;Mengwei Liang ,&nbsp;Huiping Zhao ,&nbsp;Debing Liu ,&nbsp;Guoyin Liu","doi":"10.1016/j.jplph.2025.154551","DOIUrl":"10.1016/j.jplph.2025.154551","url":null,"abstract":"<div><div>Cassava bacterial blight (CBB) poses a substantial threat to the progression and sustainability of the cassava industry. While the NLR gene family is known to play a crucial role in plant disease resistance by encoding intracellular immune receptors, the specific molecular mechanisms underlying NLR-mediated resistance in cassava remain poorly understood and require comprehensive characterization. Our research identified MeCNL3, a CC-NBS-LRR resistance gene, demonstrating significant upregulation in response to <em>Xanthomonas axonopodis</em> pv. <em>manihotis</em> (<em>Xam</em>) infection. Functional characterization revealed that <em>MeCNL3</em> overexpression confers enhanced resistance to <em>Xam</em> in cassava. Meanwhile, we demonstrated that MeCNL3 physically interacts with the transcription factor MeARF6, forming a regulatory module that controls <em>Xam</em> resistance. Notably, experimental evidence confirms that MeARF6 regulates <em>MeRbohH</em> transcription, orchestrating reactive oxygen species (ROS)-mediated defense responses against CBB. Furthermore, the type III effector protein XopR hijacks MeCNL3 to suppress the MeCNL3-MeARF6 signaling module, thereby weakening CBB resistance. Taken together, this work delineates the molecular mechanism of MeCNL3-driven CBB immunity and advances the understanding of NLR regulatory networks in cassava defense responses.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"312 ","pages":"Article 154551"},"PeriodicalIF":4.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WEE1 homolog positively regulates salt stress tolerance in chrysanthemum","authors":"Yahui Wang ,&nbsp;Fengze Sun ,&nbsp;Zhilong Bao,&nbsp;Fangfang Ma","doi":"10.1016/j.jplph.2025.154550","DOIUrl":"10.1016/j.jplph.2025.154550","url":null,"abstract":"<div><div>Salinity is a major abiotic stress that limits chrysanthemum yields worldwide. Salinity represses <em>Chrysanthemum lavandulifolium</em> plant growth and consequently reduces chrysanthemum commercial production. Salinity triggers DNA damage in root cells, leading to cell death and subsequent growth repression. <em>WEE1</em> plays an important role in regulating DNA repair, although its function in salt tolerance has not been studied in <em>C. lavandulifolium</em>. In this study, we identify <em>WEE1</em> homologous genes in <em>Chrysanthemum</em> species, and their expressions are induced in roots after salt stress treatment. We further investigate the function of <em>C. lavandulifolium</em> homolog <em>ClWEE1</em> in salt stress responses and find that <em>ClWEE1</em> plays a crucial role in cell cycle regulation and DNA damage repair under salt stress. Overexpressing <em>ClWEE1</em> in <em>C. lavandulifolium</em> or <em>Arabidopsis</em> significantly enhances their salt stress tolerance. Both flow cytometric analysis and comet assay reveal less DNA damage in <em>ClWEE1</em>-overexpression plants than in wild type. RT-qPCR analysis indicates that the stress-responsive genes <em>ClNHX</em>, <em>ClHKT</em>, <em>ClCBL</em>, and <em>ClDREB2A</em> may have higher expression in <em>ClWEE1</em>-overexpression plants than in wild type. Taken together, our study illustrates the positive role of <em>Chrysanthemum WEE1</em> in enhancing salt tolerance, providing insights for breeding salt-tolerant chrysanthemum varieties.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154550"},"PeriodicalIF":4.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physio-biochemical and molecular analyses decipher distinct dehydration stress responses in contrasting genotypes of foxtail millet (Setaria italica L.)","authors":"Laha Supriya,&nbsp;Pooja Shukla,&nbsp;Deepika Dake,&nbsp;Padmaja Gudipalli,&nbsp;Mehanathan Muthamilarasan","doi":"10.1016/j.jplph.2025.154549","DOIUrl":"10.1016/j.jplph.2025.154549","url":null,"abstract":"<div><div>Drought impairs plant growth and productivity by disrupting key physiological and biochemical processes. Foxtail millet (<em>Setaria italica</em>), a drought-resilient C₄ crop, is well-suited for climate-smart agriculture, yet its stress adaptation mechanisms remain underexplored. This study deciphered dehydration responses in tolerant and sensitive genotypes, focusing on redox regulation, sugar metabolism, energy dynamics, and autophagy. For this, four drought-distinguished millet genotypes (2 tolerant and 2 sensitive) were subjected to dehydration stress (20 % PEG-6000) for different time points (0, 2, 6 and 12 h). Tolerant genotypes exhibited improved antioxidant enzyme activity and GSH:GSSG ratios, resulting in efficient detoxification of reactive oxygen species (ROS) and improved membrane stability. Sensitive genotypes, in contrast, accumulated ROS and showed elevated oxidative damage and electrolyte leakage. Tolerant genotypes also maintained higher trans-zeatin levels and suppressed chlorophyll degradation, thereby preserving photosynthesis and delaying senescence. Sugar metabolism was more efficient in tolerant types, with increased activities of sugar metabolism enzymes, enabling proper carbohydrate partitioning and osmotic adjustment. Contrastingly, sensitive genotypes showed sugar overaccumulation due to impaired mobilization. Also, tolerant genotypes retained higher ATP and pyruvate levels, indicating better energy homeostasis. Additionally, enhanced autophagy, marked by elevated ATG8 protein and <em>ATG</em> transcript levels, supported cellular recycling in tolerant genotypes. In contrast, repressed autophagy was observed despite increased abscisic acid in sensitive genotypes, likely due to sugar-mediated signalling and elevated trehalose-6-phosphate levels. These integrated responses highlight the roles of redox control, metabolic coordination, and autophagy in dehydration tolerance and offer multi-target strategies for breeding climate-resilient <em>Setaria</em> cultivars for drought-prone environments.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154549"},"PeriodicalIF":4.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of the journey of field crop phenotyping: From trait stamp collections and fancy robots to phenomics-informed crop performance predictions","authors":"Lukas Roth,&nbsp;Afef Marzougui,&nbsp;Achim Walter","doi":"10.1016/j.jplph.2025.154542","DOIUrl":"10.1016/j.jplph.2025.154542","url":null,"abstract":"<div><div>Crop phenotyping encompasses methodologies for measuring plant growth, architecture, and composition with high precision across scales, from organs to canopies. Field-based phenotyping is pivotal in bridging genomic data with crop performance, offering a promising pathway for predictive modeling in diverse environments. This review traces the evolution of phenotyping from high-throughput sensor data for trait extraction to advanced modeling approaches that integrate multi-temporal data, latent space representations, and learned crop models. This evolution is exemplified mostly by morphology- and growth-related examples from the core expertise of the authors. High-throughput trait extraction, facilitated by advanced imaging and sensor technologies, has enabled rapid and accurate characterization of complex traits essential for crop improvement. Carrier platforms, such as drones, rovers, and gantries, have played a critical role in capturing high-resolution data across large fields, enhancing the spatial and temporal resolution of phenotypic data. Publicly available datasets have further accelerated research by providing standardized, high-quality data for benchmarking and model development beyond the realm of crop growth as for example in crop photosynthesis. These advancements are transforming phenotyping into a predictive science capable of informing breeding and management decisions. As phenotyping methodologies continue to evolve, the integration of machine learning and data-driven approaches offers new opportunities for enhancing prediction accuracy and understanding genotype-environment interactions. While challenges such as data heterogeneity, scalability, and cost remain, we highlight key gaps and propose solutions, underscoring phenotyping's critical role in future agricultural innovation.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154542"},"PeriodicalIF":4.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing light, photoperiod and temperature for accelerated flowering in speed breeding: Mechanisms, applications and crop diversity","authors":"Aneela Bashir ,&nbsp;Ansar Abbas ,&nbsp;Xiaohong Li ,&nbsp;Qingke Shi ,&nbsp;Decao Niu ,&nbsp;Lijing Zhang","doi":"10.1016/j.jplph.2025.154548","DOIUrl":"10.1016/j.jplph.2025.154548","url":null,"abstract":"<div><div>Accelerated flowering, an essential aspect of speed breeding, has become a significant tool to enhance crop improvement programs, especially in changing climates. This review examines how temperature, light quality, and photoperiod regulate flowering time across diverse crops. The mechanisms that drive these factors are being studied at the molecular, physiological, and phenotypic scales, highlighting how changes in light spectrum, photoperiod sensitivity, and temperature regimes can significantly influence flowering patterns. We emphasize the optimization of these factors in controlled environments to achieve accelerated flowering, thereby improving breeding cycles without compromising yield or plant health. The review explores the integration of these strategies into speed breeding platforms for legumes, cereals, and forage species, highlighting the challenges and potential for scaling this technology. This paper also synthesizes current knowledge and identifies understanding gaps to provide insights into strategically manipulating light quality, photoperiod, and temperature to expedite crop development and meet sustainable agriculture's demands.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154548"},"PeriodicalIF":4.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"●Dynamic changes of rice sheath non-structural carbohydrates and source-sink balance under elevated atmospheric CO2 concentration and temperature stresses","authors":"Hao He ,&nbsp;Jiabin Shi ,&nbsp;Mingjie Chen ,&nbsp;Jing Zhao ,&nbsp;Kexi Qu ,&nbsp;Man Li ,&nbsp;Lin Zhao ,&nbsp;Yuanyuan Wang ,&nbsp;Zhenghua Hu ,&nbsp;Qi Li","doi":"10.1016/j.jplph.2025.154547","DOIUrl":"10.1016/j.jplph.2025.154547","url":null,"abstract":"<div><div>Rising CO₂ levels and temperatures significantly affect rice yield and quality by altering key physiological processes. As vital carbon reserves, non-structural carbohydrates (NSC) maintain the source-sink balance, directly influencing grain filling and food security. This study simulated high CO₂ and temperature conditions using open-top chambers with four treatment groups: control (ACT, ambient CO₂ at 415 ppm and temperature), elevated CO₂ (EC, 600 ppm CO₂), elevated temperature (ET, ambient +2°C), and combined CO₂ and temperature elevation (ECT, 600 ppm CO₂ +2°C). NSC concentrations, along with key physiological indexes such as leaf nitrogen and antioxidant enzyme activity, and gene expression, was measured to assess climate impacts on <em>japonica</em> rice variety “Nanjing 9108” physiology and source-sink balance. This study found that EC enhanced NSC concentrations, increasing soluble sugars and starch by 6.33% and 9.86% at heading, raising stem sheath NSC by 9.30%. Conversely, ET reduced sugars and starch by 16.67% and 6.24%, leading to a 7.75% NSC decrease. Under ECT, NSC levels dropped by 1.07%. Nitrogen concentrations in leaves, stem sheaths, and panicles declined under both EC and ET, EC reduced leaf nitrogen by 16.26%, while ET lowered nitrogen in stem sheaths and panicles by 17.29% and 16.53%. EC upregulated <em>OsSUT1</em> and <em>OsSUT2</em> gene expression by 69.55% and 131.85%, boosting carbon transport, whereas ET suppressed those genes, reducing grain carbon supply. Overall, elevated CO₂ improves NSC accumulation and transport, enhancing yield potential, while elevated temperature hinders these processes. Managing NSC and nitrogen dynamics is crucial to ensure stable rice yields under climate change.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154547"},"PeriodicalIF":4.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Plant Alkaloid Biosynthesis under Drought Stress: Regulatory Mechanisms and Biotechnological Strategies","authors":"Ziyi Guo ,&nbsp;Sujing He ,&nbsp;Xinying Zhong ,&nbsp;Ning Yang ,&nbsp;Delin Xu","doi":"10.1016/j.jplph.2025.154545","DOIUrl":"10.1016/j.jplph.2025.154545","url":null,"abstract":"<div><div>Global climate change exacerbates drought stress, severely affecting plant growth, agricultural productivity, and the biosynthesis of secondary metabolites. Alkaloids, nitrogenous compounds with diverse biological activities, hold substantial medicinal value across various plant species. This review investigates the regulatory mechanisms through which drought stress influences alkaloid synthesis, focusing on key pathways such as abscisic acid (ABA) signaling and reactive oxygen species (ROS) responses that modulate gene expression and metabolic processes. Furthermore, we explore advanced biotechnological strategies—including genetic engineering, synthetic biology, and artificial intelligence (AI)—designed to optimizing alkaloid production under drought stress conditions. In agriculture, these strategies support the development of drought-resistant crops with enhanced alkaloid profiles, while in the pharmaceutical industry, sustainable production methods for valuable alkaloids are highlighted. The review also addresses critical challenges, such as balancing plant growth with metabolite production and ensuring field-level applicability of laboratory-developed strategies. By emphasizing interdisciplinary collaboration, this research provides comprehensive insights and practical guidance for enhancing crop resilience and maximizing alkaloid yields, thereby advancing sustainability in the medicinal plant industry.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"311 ","pages":"Article 154545"},"PeriodicalIF":4.0,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}