Journal of plant physiology最新文献

CRISPR/Cas9-mediated knockout of the 22 kDa α-prolamin genes orchestrates the regulation of functional amino acid content in foxtail millet CRISPR/ cas9介导的22 kDa α-prolamin基因敲除调控谷子功能氨基酸含量。

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-02-05 DOI: 10.1016/j.jplph.2026.154723

Rui Zhao , Jiayi Chen , Yongchao Li , Maosheng Jin , Kejin Liu , Yang Liu , Lulu Gao , Guanghui Yang , Xiangyang Yuan , Xiaoqian Chu , Jia-Gang Wang

{"title":"CRISPR/Cas9-mediated knockout of the 22 kDa α-prolamin genes orchestrates the regulation of functional amino acid content in foxtail millet","authors":"Rui Zhao , Jiayi Chen , Yongchao Li , Maosheng Jin , Kejin Liu , Yang Liu , Lulu Gao , Guanghui Yang , Xiangyang Yuan , Xiaoqian Chu , Jia-Gang Wang","doi":"10.1016/j.jplph.2026.154723","DOIUrl":"10.1016/j.jplph.2026.154723","url":null,"abstract":"<div><div>Improving the nutritional quality of cereal crops remains a primary objective in modern breeding programs. The composition and content of prolamins directly affect the overall nutritional value. This study elucidates the role of two 22 kDa α-prolamin genes (<em>Seita.9G301400</em> and <em>Seita.9G406400</em>) in foxtail millet using CRISPR/Cas9-mediated knockout. While simultaneous disruption of both genes in double mutants reduced prolamin content, amino acids, and soluble sugars, single-gene mutants exhibited the opposite effect. These single mutants displayed increased grain size alongside significantly enhanced levels of essential amino acids and sugars. Starch pasting properties were also improved in single mutants but compromised in double mutants. Our findings demonstrate that individual knockout of the two prolamin genes enhances nutritional and sensory quality, providing a potential strategy for developing improved foxtail millet varieties.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"318 ","pages":"Article 154723"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146157515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of jasmonic acid and methyl jasmonate in enhancing photosynthesis and stress response 茉莉酸和茉莉酸甲酯在提高光合作用和逆境响应中的作用。

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-02-06 DOI: 10.1016/j.jplph.2026.154725

Saykat Dey Sonatan, Jibon Kumar Paul, Mahir Azmal, ANM Shah Newaz Been Haque, Ajit Ghosh

引用次数: 0

Biochemical memory in plants: A missing component in climate resilience models 植物的生化记忆：气候适应模型中缺失的部分。

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-02-12 DOI: 10.1016/j.jplph.2026.154728

Gayatri Mishra

{"title":"Biochemical memory in plants: A missing component in climate resilience models","authors":"Gayatri Mishra","doi":"10.1016/j.jplph.2026.154728","DOIUrl":"10.1016/j.jplph.2026.154728","url":null,"abstract":"<div><div>Plants do not rapidly return to their pre-stress state; instead, they retain physiological and biochemical traces of past environmental conditions. Biochemical memory refers to the persistence of stress-induced metabolic and physiological states that influence how plants respond to subsequent environmental challenges. Shifts in osmolytes, redox balance, secondary metabolites, and hormonal sensitivity can persist beyond the stress event, shaping how plants respond to subsequent drought, heat, or nutrient fluctuations. Yet in most climate and vegetation models, stress is treated as a transient reduction in function, after which plants are assumed to recover to a fixed baseline. Because these models lack mechanisms to represent physiological legacies, they implicitly overlook the biochemical memory that influences growth, carbon–water exchange, and resilience. This disconnect limits our ability to predict how vegetation and ecosystems will behave under increasing climatic variability. In this Opinion, I outline how biochemical memory arises, how it influences plant behaviour across scales, and why incorporating this history into modelling frameworks is essential. Recognising that plants operate with a memory of past stress is crucial for producing realistic forecasts in a rapidly changing climate.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"318 ","pages":"Article 154728"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146197838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid genetic transformation of herbaceous peony without tissue culture via Agrobacterium rhizogenes: Optimization using rhizomes, stems, roots, and seedlings 根农杆菌对芍药无组织培养的快速遗传转化：根茎、茎、根和苗的优化

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-01-22 DOI: 10.1016/j.jplph.2026.154709

Xi Chen , Jiageng Zhao , Chenchen Ji , Tinglei Du , Meiting Wang , Jingyi Shu , Jaime A. Teixeira da Silvai , Xiaonan Yu

{"title":"Rapid genetic transformation of herbaceous peony without tissue culture via Agrobacterium rhizogenes: Optimization using rhizomes, stems, roots, and seedlings","authors":"Xi Chen , Jiageng Zhao , Chenchen Ji , Tinglei Du , Meiting Wang , Jingyi Shu , Jaime A. Teixeira da Silvai , Xiaonan Yu","doi":"10.1016/j.jplph.2026.154709","DOIUrl":"10.1016/j.jplph.2026.154709","url":null,"abstract":"<div><div>Herbaceous peony is a famous traditional flower worldwide. However, the lack of a robust transgenic system has severely restricted its genetic improvement efforts. In this study, we established a tissue culture-free <em>Agrobacterium rhizogenes</em>-mediated transformation system using the rhizomes, stems, root segments, and seedlings of herbaceous peony. Soaking rhizomes and root segments in a resuspension solution containing acetosyringone and 2-morpholinoethanesulfonic acid induced more GFP-positive hairy roots than that in other tissues. Our study also revealed that colony smear was the optimal infection method for stems and seedlings, that one-year-old seedlings were most susceptible to infection, and that <em>Agrobacterium</em> strain K599 was more effective than MSU440 and C58C1. Among the 11 cultivars, even though all formed hairy roots, 'Dafugui' of the Lactiflora group showed the highest transgenic efficiency. This study provides a rapid and efficient tissue culture-free strategy for the genetic transformation of herbaceous peony, providing an important basis for its molecular breeding.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"318 ","pages":"Article 154709"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanisms by which polyamines regulate wheat grain filling under drought stress conditions 干旱胁迫下多胺调节小麦籽粒灌浆的机制

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-01-09 DOI: 10.1016/j.jplph.2026.154693

Sara Sadat Afjeh, Pouria Mostafaie, Ali Ahmadi, Ali Reza Abbasi

{"title":"The mechanisms by which polyamines regulate wheat grain filling under drought stress conditions","authors":"Sara Sadat Afjeh, Pouria Mostafaie, Ali Ahmadi, Ali Reza Abbasi","doi":"10.1016/j.jplph.2026.154693","DOIUrl":"10.1016/j.jplph.2026.154693","url":null,"abstract":"<div><div>Drought stress (DS) is a major factor limiting wheat grain filling. Polyamines (PAs) play crucial roles in plant responses to DS; however, the mechanisms underlying their effects on grain filling are not fully understood. This study aimed to clarify the regulatory role of PAs in grain filling through source-sink dynamics in two wheat cultivars representing distinct drought tolerance under non-stress and DS conditions, with or without exogenous spermine and putrescine. Data indicated that DS significantly disrupted the grain-filling process (<em>P</em> < 0.01), accompanied by severe limitations in source-sink capacity. However, PA application improved chlorophyll content (9.45–23.39 %), Fv/Fm values (1.86–5.56 %), assimilate partitioning to non-structural carbohydrates (4.24–7.17 %), and stem reserves (6.42–24.44 %), thereby enhancing source capacity. PAs also reduced abscisic acid (ABA) levels during early grain-filling stages and increased auxin and cytokinin levels, which were associated with enhanced endosperm cell division and number (<em>P</em> < 0.05), thereby improving sink capacity. In later grain-filling stages, PAs caused a controlled increase in ABA levels, serving as physiological signals for reserve mobilization and significantly inducing the expression of <em>1-FEH-w3</em> and <em>SPSI</em> genes (<em>P</em> < 0.01). These changes were accompanied by improved stem reserve remobilization (RM) (6–16.70 %), grain-filling rate, and grain yield (<em>P</em> < 0.05). The cultivars' responses to spermine application were more evident than to putrescine, particularly in the sensitive cultivar. Overall, PAs could significantly enhance grain filling and sustain wheat yield under DS conditions, likely through a multifaceted mechanism involving hormonal regulation, maintaining source-sink capacity, and facilitating RM.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"318 ","pages":"Article 154693"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146080220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Routing resilience: Engineering metabolite transport for combined drought and heavy-metal tolerance in plants 路由弹性：工程代谢物运输对植物干旱和重金属综合耐受性。

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-01-21 DOI: 10.1016/j.jplph.2026.154711

Gayatri Mishra

{"title":"Routing resilience: Engineering metabolite transport for combined drought and heavy-metal tolerance in plants","authors":"Gayatri Mishra","doi":"10.1016/j.jplph.2026.154711","DOIUrl":"10.1016/j.jplph.2026.154711","url":null,"abstract":"<div><div>Plants can experience individual stresses such as drought or heavy-metal exposure, yet in many environments these factors co-occur, imposing conflicting demands on water conservation, ion homeostasis, and metabolic detoxification. While biosynthetic pathways for stress-responsive metabolites are well studied, the regulation and engineering of metabolite transport remain largely overlooked. Transporters such as ABC, MATE, NPF, SWEET, and ALMT determine how osmolytes, antioxidants, and chelators are distributed across tissues and the rhizosphere, shaping physiological outcomes under stress. This Opinion highlights metabolite transport as a missing regulatory layer linking drought physiology and metal detoxification networks. I propose a Cross-stress Metabolite-Transport Engineering (CoMET) framework that treats these transporters as programmable valves to optimize metabolite fluxes under combined stress. CoMET integrates flux diagnostics, synthetic promoter logic, transporter editing, and field-based learning loops. Recognizing and engineering metabolite transport as a dynamic control system could redefine how crops maintain both water relations and detoxification capacity in increasingly contaminated and drought-prone soils.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"318 ","pages":"Article 154711"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting plant isoprene emission: From atmospheric chemistry to plant stress resilience 重访植物异戊二烯排放：从大气化学到植物抗逆性。

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1016/j.jplph.2026.154712

Thomas D. Sharkey , Manuel Bellucci , Francesco Loreto , Mohammad G. Mostofa , Abira Sahu , Bianca M.L. Serda , Sarathi M. Weraduwage , Yuan Xu

{"title":"Revisiting plant isoprene emission: From atmospheric chemistry to plant stress resilience","authors":"Thomas D. Sharkey , Manuel Bellucci , Francesco Loreto , Mohammad G. Mostofa , Abira Sahu , Bianca M.L. Serda , Sarathi M. Weraduwage , Yuan Xu","doi":"10.1016/j.jplph.2026.154712","DOIUrl":"10.1016/j.jplph.2026.154712","url":null,"abstract":"<div><div>The emission of isoprene from plants was first discovered in the 1950s but was relatively unknown in the plant science community until the 1990s. Isoprene is the five-carbon branched molecule that is the root member of the extensive family of isoprenoids. The amount of isoprene from plants exceeds all other hydrocarbon inputs to the atmosphere. Plant-emitted isoprene can affect ozone formation and often increases (but can decrease) growth of aerosols (particles in the atmosphere). The rate of isoprene emission is estimated using empirical or mechanistic modeling has been used to predict global emissions. Beyond its atmospheric role, isoprene can improve plant resilience to biotic and abiotic stress, likely through interactions with transcriptional networks that regulate plant growth and defense. Isoprene has all the properties of the five compounds classically described as plant hormones. These and an additional four molecules are now called small molecule plant growth regulators, and we propose that isoprene also belongs to this group. Plants previously thought to lack the capacity for isoprene emission have now been found that make isoprene in response to leaf damage. This discovery suggests that many plants once classified as non-emitters likely have the capacity to emit isoprene under specific conditions. This review summarizes past and current understanding of the biosynthesis and regulatory mechanisms, atmospheric significance, and physiological roles of isoprene emitted from plants.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"318 ","pages":"Article 154712"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146125083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor OsERF74 positively regulates drought resistance by modulating abscisic acid catabolism in rice 转录因子OsERF74通过调控水稻脱落酸分解代谢正向调节水稻抗旱性

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-01-20 DOI: 10.1016/j.jplph.2026.154710

Ziyi Luo , Chen Tang , Liping Wang , Xiaoyu Sha , Yuhan Zhang , Wenjiang Liu , Jingye Fu , Qiang Wang

{"title":"The transcription factor OsERF74 positively regulates drought resistance by modulating abscisic acid catabolism in rice","authors":"Ziyi Luo , Chen Tang , Liping Wang , Xiaoyu Sha , Yuhan Zhang , Wenjiang Liu , Jingye Fu , Qiang Wang","doi":"10.1016/j.jplph.2026.154710","DOIUrl":"10.1016/j.jplph.2026.154710","url":null,"abstract":"<div><div>Against the backdrop of global climate change, water scarcity and food shortages, drought has emerged as a critical constraint on crop productivity, posing a severe threat to sustainable agricultural production. In this study, we identify the rice transcription factor OsERF74 as a key regulator of drought resistance. Overexpression of <em>OsERF74</em> in Arabidopsis plants enhances drought tolerance, whereas rice knockout lines display increased drought sensitivity. Transcriptomic analysis reveals that OsERF74 modulates multiple pathways under drought stress. Mechanistically, OsERF74 directly binds to the promoters of ABA catabolic genes <em>OsABA8ox1&2</em> to regulate their expression, thereby modulating ABA homeostasis and drought responses. Our findings demonstrate that OsERF74 positively regulates drought resistance by directly controlling ABA degradation, as well as regulating multiple signaling pathways. This study provides a critical scientific foundation for improving crop drought tolerance and ensuring food security.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"318 ","pages":"Article 154710"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing one-step hairy root transformation system in safflower using RUBY reporter 利用RUBY报告程序建立红花毛状根一步转化系统。

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1016/j.jplph.2026.154713

Rong Guo, Xuerui Zhang, Xu Jiao, Chunfeng Zhu, Jian Wei, Yun Zhu

引用次数: 0

MdCNGC15B, a cyclic nucleotide-gated channel (CNGC) protein, positively regulates the tolerance to drought and salt stress in apple 环核苷酸门控通道（CNGC）蛋白MdCNGC15B正调控苹果对干旱和盐胁迫的耐性。

IF 4.1 3区生物学

Journal of plant physiology Pub Date : 2026-03-01 Epub Date: 2026-02-05 DOI: 10.1016/j.jplph.2026.154722

Xin-Long Guo , Hong-Liang Li , Xiang Wu , Tian-tian Wang , Yan-yan Guo , Jian-Ping An , Chun-Xiang You

引用次数: 0